LOCUS       DAZKAY010000001        76150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000001 DAZKAY010000000
VERSION     DAZKAY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.001"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACG8WP_000001"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACG8WP_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9026347.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACG8WP_000002"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACG8WP_000002"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9026348.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACG8WP_000003"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACG8WP_000003"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9026349.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5738..7039
                     /locus_tag="ACG8WP_000004"
     CDS             5738..7039
                     /locus_tag="ACG8WP_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9026350.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7108..7707)
                     /locus_tag="ACG8WP_000005"
     CDS             complement(7108..7707)
                     /locus_tag="ACG8WP_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9026351.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7962..8843
                     /locus_tag="ACG8WP_000006"
     CDS             7962..8843
                     /locus_tag="ACG8WP_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9026352.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8856..10466
                     /locus_tag="ACG8WP_000007"
     CDS             8856..10466
                     /locus_tag="ACG8WP_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026353.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10543..11202)
                     /locus_tag="ACG8WP_000008"
     CDS             complement(10543..11202)
                     /locus_tag="ACG8WP_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9026354.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11485..12150)
                     /locus_tag="ACG8WP_000009"
     CDS             complement(11485..12150)
                     /locus_tag="ACG8WP_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9026355.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="ACG8WP_000010"
     CDS             complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="ACG8WP_000010"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9026356.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="ACG8WP_000011"
     CDS             complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="ACG8WP_000011"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9026357.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="ACG8WP_000012"
     CDS             complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="ACG8WP_000012"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9026358.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14464..14784
                     /locus_tag="ACG8WP_000013"
     CDS             14464..14784
                     /locus_tag="ACG8WP_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9026359.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14896..15753)
                     /locus_tag="ACG8WP_000014"
     CDS             complement(14896..15753)
                     /locus_tag="ACG8WP_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9026360.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(15861..17165)
                     /locus_tag="ACG8WP_000015"
     CDS             complement(15861..17165)
                     /locus_tag="ACG8WP_000015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9026361.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17162..17671)
                     /locus_tag="ACG8WP_000016"
     CDS             complement(17162..17671)
                     /locus_tag="ACG8WP_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026362.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17846..19981)
                     /locus_tag="ACG8WP_000017"
     CDS             complement(17846..19981)
                     /locus_tag="ACG8WP_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9026363.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20064..21719)
                     /locus_tag="ACG8WP_000018"
     CDS             complement(20064..21719)
                     /locus_tag="ACG8WP_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9026364.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21721..22035)
                     /locus_tag="ACG8WP_000019"
     CDS             complement(21721..22035)
                     /locus_tag="ACG8WP_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9026365.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22205..22465
                     /locus_tag="ACG8WP_000020"
     CDS             22205..22465
                     /locus_tag="ACG8WP_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9026366.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22479..22793
                     /locus_tag="ACG8WP_000021"
     CDS             22479..22793
                     /locus_tag="ACG8WP_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9026367.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23523..24077)
                     /locus_tag="ACG8WP_000022"
     CDS             complement(23523..24077)
                     /locus_tag="ACG8WP_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9026368.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24203..26132)
                     /locus_tag="ACG8WP_000023"
                     /pseudo
     CDS             complement(24203..26132)
                     /locus_tag="ACG8WP_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26438..27154)
                     /locus_tag="ACG8WP_000024"
     CDS             complement(26438..27154)
                     /locus_tag="ACG8WP_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9026369.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="ACG8WP_000025"
     CDS             complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="ACG8WP_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9026370.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="ACG8WP_000026"
     CDS             complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="ACG8WP_000026"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9026371.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30624..32084)
                     /locus_tag="ACG8WP_000027"
     CDS             complement(30624..32084)
                     /locus_tag="ACG8WP_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026372.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32074..33369)
                     /locus_tag="ACG8WP_000028"
     CDS             complement(32074..33369)
                     /locus_tag="ACG8WP_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9026373.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33701..34378)
                     /locus_tag="ACG8WP_000029"
     CDS             complement(33701..34378)
                     /locus_tag="ACG8WP_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9026374.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34408..35499)
                     /locus_tag="ACG8WP_000030"
     CDS             complement(34408..35499)
                     /locus_tag="ACG8WP_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9026375.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35600..36034)
                     /locus_tag="ACG8WP_000031"
     CDS             complement(35600..36034)
                     /locus_tag="ACG8WP_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9026376.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36618..38075)
                     /locus_tag="ACG8WP_000032"
     CDS             complement(36618..38075)
                     /locus_tag="ACG8WP_000032"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9026377.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38072..39967)
                     /locus_tag="ACG8WP_000033"
     CDS             complement(38072..39967)
                     /locus_tag="ACG8WP_000033"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9026378.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40025..41800)
                     /locus_tag="ACG8WP_000034"
     CDS             complement(40025..41800)
                     /locus_tag="ACG8WP_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9026379.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41850..43346)
                     /locus_tag="ACG8WP_000035"
     CDS             complement(41850..43346)
                     /locus_tag="ACG8WP_000035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9026380.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43540..45162)
                     /locus_tag="ACG8WP_000036"
     CDS             complement(43540..45162)
                     /locus_tag="ACG8WP_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9026381.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45490..46803)
                     /locus_tag="ACG8WP_000037"
     CDS             complement(45490..46803)
                     /locus_tag="ACG8WP_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9026382.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47048..47272)
                     /locus_tag="ACG8WP_000038"
     CDS             complement(47048..47272)
                     /locus_tag="ACG8WP_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026383.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47292..47714)
                     /locus_tag="ACG8WP_000039"
     CDS             complement(47292..47714)
                     /locus_tag="ACG8WP_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9026384.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(47856..48566)
                     /locus_tag="ACG8WP_000040"
     CDS             complement(47856..48566)
                     /locus_tag="ACG8WP_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9026385.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48584..49081)
                     /locus_tag="ACG8WP_000041"
     CDS             complement(48584..49081)
                     /locus_tag="ACG8WP_000041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9026386.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49118..49570)
                     /locus_tag="ACG8WP_000042"
     CDS             complement(49118..49570)
                     /locus_tag="ACG8WP_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9026387.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49705..50310)
                     /locus_tag="ACG8WP_000043"
     CDS             complement(49705..50310)
                     /locus_tag="ACG8WP_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9026388.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50760..51380)
                     /locus_tag="ACG8WP_000044"
     CDS             complement(50760..51380)
                     /locus_tag="ACG8WP_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9026389.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51382..52251)
                     /locus_tag="ACG8WP_000045"
     CDS             complement(51382..52251)
                     /locus_tag="ACG8WP_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9026390.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52274..52681)
                     /locus_tag="ACG8WP_000046"
     CDS             complement(52274..52681)
                     /locus_tag="ACG8WP_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9026391.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52700..53989)
                     /locus_tag="ACG8WP_000047"
     CDS             complement(52700..53989)
                     /locus_tag="ACG8WP_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9026392.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(54016..54318)
                     /locus_tag="ACG8WP_000048"
     CDS             complement(54016..54318)
                     /locus_tag="ACG8WP_000048"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9026393.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(54343..56436)
                     /locus_tag="ACG8WP_000049"
     CDS             complement(54343..56436)
                     /locus_tag="ACG8WP_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9026394.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(56925..57689)
                     /locus_tag="ACG8WP_000050"
     CDS             complement(56925..57689)
                     /locus_tag="ACG8WP_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9026395.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="ACG8WP_000051"
     CDS             complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="ACG8WP_000051"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9026396.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(58694..59770)
                     /locus_tag="ACG8WP_000052"
     CDS             complement(58694..59770)
                     /locus_tag="ACG8WP_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9026397.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(59794..60387)
                     /locus_tag="ACG8WP_000053"
     CDS             complement(59794..60387)
                     /locus_tag="ACG8WP_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9026398.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="ACG8WP_000054"
     CDS             complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="ACG8WP_000054"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9026399.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(62057..62356)
                     /locus_tag="ACG8WP_000055"
     CDS             complement(62057..62356)
                     /locus_tag="ACG8WP_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9026400.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="ACG8WP_000056"
     CDS             complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="ACG8WP_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9026401.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(64111..65022)
                     /locus_tag="ACG8WP_000057"
     CDS             complement(64111..65022)
                     /locus_tag="ACG8WP_000057"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9026402.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            65142..65999
                     /locus_tag="ACG8WP_000058"
     CDS             65142..65999
                     /locus_tag="ACG8WP_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9026403.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(66186..66548)
                     /locus_tag="ACG8WP_000059"
     CDS             complement(66186..66548)
                     /locus_tag="ACG8WP_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9026404.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(66737..66919)
                     /locus_tag="ACG8WP_000060"
     CDS             complement(66737..66919)
                     /locus_tag="ACG8WP_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026405.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMSLKNDTTKIKK"
     gene            66894..68333
                     /locus_tag="ACG8WP_000061"
     CDS             66894..68333
                     /locus_tag="ACG8WP_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026406.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(68340..70892)
                     /locus_tag="ACG8WP_000062"
     CDS             complement(68340..70892)
                     /locus_tag="ACG8WP_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9026407.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(71006..72337)
                     /locus_tag="ACG8WP_000063"
     CDS             complement(71006..72337)
                     /locus_tag="ACG8WP_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9026408.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(72303..73781)
                     /locus_tag="ACG8WP_000064"
     CDS             complement(72303..73781)
                     /locus_tag="ACG8WP_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9026409.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="ACG8WP_000065"
     CDS             complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="ACG8WP_000065"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9026410.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        21774 a        16164 c        13052 g        25160 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
     5881 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
     5941 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
     6001 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
     6061 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
     6121 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
     6181 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
     6241 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
     6301 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
     6361 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
     6421 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
     6481 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
     6541 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
     6601 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
     6661 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
     6721 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
     6781 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
     6841 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
     6901 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
     6961 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
     7021 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
     7081 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
     7141 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
     7201 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
     7261 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
     7321 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
     7381 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
     7441 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
     7501 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
     7561 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
     7621 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
     7681 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
     7741 atatatatag ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa
     7801 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
     7861 taaaataata aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
     7921 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
     7981 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
     8041 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
     8101 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
     8161 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
     8221 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
     8281 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
     8341 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
     8401 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
     8461 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
     8521 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
     8581 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
     8641 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
     8701 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
     8761 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
     8821 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
     8881 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     8941 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     9001 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     9061 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     9121 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     9181 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     9241 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     9301 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     9361 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     9421 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     9481 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     9541 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     9601 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     9661 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     9721 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     9781 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     9841 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     9901 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     9961 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
    10021 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
    10081 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
    10141 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
    10201 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
    10261 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
    10321 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
    10381 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
    10441 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
    10501 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
    10561 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
    10621 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
    10681 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
    10741 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
    10801 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
    10861 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
    10921 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
    10981 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
    11041 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
    11101 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
    11161 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
    11221 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
    11281 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
    11341 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
    11401 tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt
    11461 ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag
    11521 tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg
    11581 atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc
    11641 ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc
    11701 tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat
    11761 cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg
    11821 gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc
    11881 gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt
    11941 cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt
    12001 ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc
    12061 aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa
    12121 caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat
    12181 tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag
    12241 tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa
    12301 ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg
    12361 tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag
    12421 actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat
    12481 gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct
    12541 cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca
    12601 atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga
    12661 ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg
    12721 attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg
    12781 ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc
    12841 tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc
    12901 atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct
    12961 aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata
    13021 tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc
    13081 aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt
    13141 ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta
    13201 acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca
    13261 ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct
    13321 acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc
    13381 ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg
    13441 attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt
    13501 tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct
    13561 aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa
    13621 actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa
    13681 gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg
    13741 gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc
    13801 ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat
    13861 ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc
    13921 ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat
    13981 accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata
    14041 acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct
    14101 accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac
    14161 gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac
    14221 ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact
    14281 atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct
    14341 aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg
    14401 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
    14461 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
    14521 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
    14581 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
    14641 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
    14701 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
    14761 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
    14821 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
    14881 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
    14941 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
    15001 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
    15061 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
    15121 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
    15181 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
    15241 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
    15301 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
    15361 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
    15421 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
    15481 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
    15541 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
    15601 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
    15661 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
    15721 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
    15781 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
    15841 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
    15901 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
    15961 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
    16021 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
    16081 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
    16141 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
    16201 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
    16261 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
    16321 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
    16381 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
    16441 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
    16501 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
    16561 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
    16621 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
    16681 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
    16741 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
    16801 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
    16861 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
    16921 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
    16981 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
    17041 acataacacc agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga
    17101 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
    17161 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
    17221 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
    17281 gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt
    17341 caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg
    17401 aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt
    17461 tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg
    17521 ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg
    17581 tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg
    17641 atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac
    17701 gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa
    17761 gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt
    17821 gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca
    17881 ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt
    17941 caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga
    18001 tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt
    18061 cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag
    18121 ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt
    18181 gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct
    18241 ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat
    18301 ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct
    18361 gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta
    18421 attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat
    18481 ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac
    18541 gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact
    18601 gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt
    18661 gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac
    18721 cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac
    18781 tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt
    18841 gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt
    18901 ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta
    18961 aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg
    19021 tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt
    19081 cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac
    19141 ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca
    19201 gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca
    19261 tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca
    19321 catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat
    19381 tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat
    19441 caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg
    19501 tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa
    19561 gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat
    19621 ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt
    19681 ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt
    19741 tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat
    19801 ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg
    19861 actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa
    19921 ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca
    19981 tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc
    20041 gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca
    20101 gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag
    20161 attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg
    20221 ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg
    20281 aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct
    20341 agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt
    20401 gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc
    20461 aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata
    20521 tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc
    20581 tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga
    20641 tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca
    20701 cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg
    20761 ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg
    20821 gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata
    20881 gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata
    20941 cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca
    21001 ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt
    21061 ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga
    21121 gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt
    21181 gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat
    21241 ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc
    21301 actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct
    21361 ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca
    21421 atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc
    21481 acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg
    21541 acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt
    21601 gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg
    21661 cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc
    21721 ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac
    21781 atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca
    21841 aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac
    21901 atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa
    21961 cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg
    22021 aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc atggggtata
    22081 ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt
    22141 ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg
    22201 tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat
    22261 tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt
    22321 aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa
    22381 aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc
    22441 gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg
    22501 atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc
    22561 cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg
    22621 ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc
    22681 gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg
    22741 tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag
    22801 aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact
    22861 tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca
    22921 gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa
    22981 gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga
    23041 gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
    23101 agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta
    23161 agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga
    23221 aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
    23281 ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga
    23341 aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat
    23401 cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga
    23461 gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt
    23521 ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa
    23581 agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa
    23641 agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc
    23701 gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct
    23761 tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac
    23821 aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct
    23881 gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca
    23941 acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc
    24001 agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag
    24061 tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag
    24121 cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat
    24181 attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct
    24241 gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt
    24301 cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg
    24361 ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt
    24421 ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt
    24481 tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat
    24541 tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg
    24601 ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat
    24661 gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga
    24721 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    24781 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    24841 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    24901 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    24961 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    25021 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    25081 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    25141 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    25201 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    25261 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    25321 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    25381 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    25441 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    25501 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    25561 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    25621 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    25681 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    25741 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    25801 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    25861 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    25921 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    25981 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    26041 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    26101 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    26161 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    26221 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    26281 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    26341 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    26401 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    26461 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    26521 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    26581 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    26641 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    26701 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    26761 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    26821 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    26881 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    26941 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    27001 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    27061 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    27121 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    27181 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    27241 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    27301 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    27361 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    27421 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    27481 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    27541 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    27601 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    27661 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    27721 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    27781 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    27841 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    27901 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    27961 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    28021 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    28081 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    28141 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    28201 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    28261 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    28321 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    28381 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    28441 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    28501 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    28561 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    28621 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    28681 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    28741 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    28801 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    28861 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    28921 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    28981 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    29041 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    29101 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    29161 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    29221 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    29281 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    29341 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    29401 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    29461 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    29521 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    29581 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    29641 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    29701 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    29761 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    29821 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    29881 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    29941 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    30001 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    30061 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    30121 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    30181 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    30241 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    30301 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    30361 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    30421 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    30481 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    30541 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    30601 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    30661 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    30721 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    30781 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    30841 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    30901 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    30961 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    31021 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    31081 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    31141 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    31201 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    31261 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    31321 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    31381 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    31441 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    31501 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    31561 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    31621 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    31681 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    31741 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    31801 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    31861 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    31921 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    31981 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    32041 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    32101 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    32161 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    32221 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    32281 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    32341 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    32401 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    32461 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    32521 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    32581 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    32641 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
    32701 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    32761 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    32821 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    32881 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    32941 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    33001 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    33061 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    33121 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    33181 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    33241 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    33301 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    33361 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    33421 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    33481 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    33541 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    33601 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    33661 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    33721 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    33781 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    33841 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    33901 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    33961 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    34021 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    34081 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    34141 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    34201 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    34261 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    34321 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    34381 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    34441 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    34501 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    34561 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    34621 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    34681 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    34741 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    34801 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    34861 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    34921 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    34981 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    35041 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    35101 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    35161 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    35221 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    35281 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    35341 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    35401 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    35461 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    35521 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    35581 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    35641 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    35701 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    35761 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    35821 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    35881 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    35941 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    36001 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    36061 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    36121 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    36181 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    36241 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    36301 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    36361 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    36421 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    36481 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    36541 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    36601 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    36661 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    36721 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    36781 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    36841 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    36901 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    36961 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    37021 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    37081 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    37141 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    37201 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    37261 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    37321 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    37381 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    37441 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    37501 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    37561 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    37621 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    37681 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    37741 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    37801 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    37861 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    37921 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    37981 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    38041 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    38101 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    38161 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    38221 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    38281 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    38341 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    38401 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    38461 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    38521 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    38581 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    38641 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    38701 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    38761 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    38821 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    38881 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    38941 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    39001 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    39061 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    39121 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    39181 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    39241 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    39301 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    39361 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    39421 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    39481 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    39541 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    39601 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    39661 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    39721 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    39781 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    39841 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    39901 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    39961 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    40021 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    40081 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    40141 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    40201 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    40261 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    40321 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    40381 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    40441 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    40501 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    40561 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    40621 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    40681 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    40741 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    40801 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    40861 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    40921 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    40981 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    41041 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    41101 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    41161 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    41221 aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa
    41281 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    41341 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    41401 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    41461 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    41521 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    41581 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    41641 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    41701 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    41761 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    41821 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    41881 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    41941 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    42001 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    42061 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    42121 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    42181 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    42241 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    42301 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    42361 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    42421 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    42481 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    42541 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    42601 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    42661 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    42721 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    42781 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    42841 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    42901 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    42961 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    43021 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    43081 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    43141 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    43201 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    43261 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    43321 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    43381 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    43441 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    43501 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    43561 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    43621 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    43681 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    43741 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    43801 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    43861 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    43921 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    43981 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    44041 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    44101 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    44161 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    44221 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    44281 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    44341 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    44401 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    44461 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    44521 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    44581 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    44641 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    44701 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    44761 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    44821 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    44881 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    44941 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    45001 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    45061 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    45121 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    45181 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    45241 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    45301 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    45361 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    45421 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    45481 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    45541 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    45601 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    45661 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    45721 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    45781 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    45841 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    45901 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    45961 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    46021 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    46081 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    46141 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    46201 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    46261 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    46321 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    46381 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    46441 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    46501 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    46561 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    46621 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    46681 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    46741 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    46801 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    46861 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    46921 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    46981 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    47041 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    47101 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    47161 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    47221 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    47281 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    47341 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    47401 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    47461 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    47521 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    47581 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    47641 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    47701 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    47761 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    47821 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    47881 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    47941 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    48001 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
    48061 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    48121 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    48181 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    48241 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    48301 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    48361 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    48421 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    48481 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    48541 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    48601 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    48661 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    48721 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    48781 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    48841 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    48901 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    48961 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    49021 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    49081 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    49141 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    49201 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    49261 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    49321 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    49381 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    49441 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    49501 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    49561 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
    49621 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    49681 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    49741 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    49801 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    49861 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    49921 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    49981 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    50041 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    50101 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    50161 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    50221 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    50281 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    50341 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    50401 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    50461 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    50521 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    50581 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    50641 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    50701 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    50761 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    50821 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    50881 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    50941 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    51001 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
    51061 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    51121 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    51181 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    51241 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    51301 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    51361 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    51421 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    51481 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    51541 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    51601 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    51661 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    51721 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    51781 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    51841 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    51901 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    51961 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    52021 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    52081 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    52141 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    52201 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    52261 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    52321 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    52381 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    52441 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    52501 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    52561 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    52621 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    52681 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    52741 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat
    52801 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    52861 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    52921 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    52981 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    53041 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    53101 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    53161 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    53221 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    53281 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    53341 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    53401 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    53461 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    53521 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    53581 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    53641 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    53701 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    53761 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    53821 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    53881 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    53941 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    54001 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    54061 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    54121 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    54181 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    54241 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    54301 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    54361 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    54421 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    54481 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    54541 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    54601 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    54661 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    54721 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    54781 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    54841 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    54901 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    54961 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    55021 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    55081 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    55141 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    55201 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    55261 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    55321 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    55381 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    55441 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    55501 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    55561 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    55621 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    55681 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    55741 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    55801 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    55861 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    55921 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    55981 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    56041 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    56101 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    56161 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    56221 agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg tacggatctc
    56281 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    56341 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    56401 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    56461 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    56521 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    56581 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    56641 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    56701 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    56761 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    56821 aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag aaaaaggagc
    56881 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    56941 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    57001 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    57061 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    57121 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    57181 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    57241 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    57301 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    57361 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    57421 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    57481 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    57541 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    57601 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    57661 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    57721 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    57781 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    57841 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    57901 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    57961 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    58021 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    58081 gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca agcctgatat
    58141 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    58201 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    58261 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    58321 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    58381 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    58441 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    58501 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    58561 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    58621 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    58681 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    58741 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    58801 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    58861 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    58921 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    58981 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    59041 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    59101 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    59161 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    59221 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    59281 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    59341 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    59401 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    59461 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    59521 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    59581 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    59641 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    59701 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    59761 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    59821 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    59881 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    59941 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    60001 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    60061 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    60121 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    60181 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    60241 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    60301 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    60361 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    60421 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    60481 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    60541 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    60601 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    60661 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    60721 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    60781 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    60841 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    60901 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    60961 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    61021 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    61081 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    61141 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    61201 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    61261 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    61321 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    61381 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    61441 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    61501 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    61561 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    61621 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    61681 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    61741 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    61801 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    61861 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    61921 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    61981 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    62041 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    62101 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    62161 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    62221 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    62281 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    62341 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    62401 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    62461 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    62521 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    62581 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    62641 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    62701 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    62761 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    62821 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    62881 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    62941 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    63001 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    63061 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    63121 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    63181 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    63241 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    63301 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    63361 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    63421 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    63481 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    63541 gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    63601 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    63661 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    63721 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    63781 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    63841 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    63901 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    63961 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    64021 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    64081 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    64141 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    64201 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    64261 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    64321 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    64381 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    64441 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    64501 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    64561 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    64621 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    64681 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    64741 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    64801 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    64861 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    64921 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    64981 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    65041 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    65101 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    65161 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    65221 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    65281 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    65341 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    65401 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    65461 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    65521 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    65581 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    65641 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    65701 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    65761 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    65821 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    65881 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    65941 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa
    66001 aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca
    66061 gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta
    66121 aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta
    66181 gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt
    66241 tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa
    66301 tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata
    66361 ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg
    66421 accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta
    66481 aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc
    66541 gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag
    66601 aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc
    66661 aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt
    66721 attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag ctcatgatga
    66781 aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa
    66841 cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt
    66901 cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac
    66961 aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg
    67021 aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca
    67081 tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta acaaatgacc
    67141 tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta
    67201 caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga
    67261 aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga
    67321 cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat
    67381 tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc
    67441 attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga
    67501 ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag
    67561 attcttctat ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat
    67621 tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct
    67681 ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc
    67741 attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc
    67801 ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc
    67861 atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat
    67921 atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa
    67981 aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac
    68041 aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac
    68101 cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct
    68161 tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga
    68221 tttccaattt ccctctagat tctctagata tccctattgt atacatttct tctattccaa
    68281 cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc
    68341 tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct
    68401 ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt
    68461 gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc
    68521 ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct
    68581 tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag
    68641 acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat
    68701 aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca
    68761 gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac
    68821 tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    68881 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    68941 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    69001 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    69061 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    69121 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    69181 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    69241 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    69301 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    69361 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    69421 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    69481 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    69541 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    69601 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    69661 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    69721 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    69781 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    69841 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    69901 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    69961 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    70021 atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc
    70081 gaaagaagaa tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    70141 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    70201 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    70261 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    70321 cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    70381 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    70441 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    70501 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    70561 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    70621 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    70681 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    70741 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    70801 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    70861 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    70921 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    70981 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    71041 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    71101 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    71161 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    71221 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    71281 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    71341 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    71401 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    71461 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    71521 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    71581 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    71641 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    71701 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    71761 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    71821 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    71881 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    71941 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    72001 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    72061 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    72121 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    72181 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    72241 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    72301 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    72361 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    72421 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    72481 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    72541 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    72601 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    72661 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    72721 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    72781 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    72841 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    72901 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    72961 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    73021 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    73081 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    73141 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    73201 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    73261 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    73321 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    73381 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    73441 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    73501 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    73561 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    73621 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    73681 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    73741 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    73801 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    73861 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    73921 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    73981 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    74041 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    74101 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    74161 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    74221 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    74281 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    74341 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    74401 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    74461 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    74521 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    74581 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    74641 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    74701 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    74761 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    74821 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    74881 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    74941 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    75001 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    75061 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    75121 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    75181 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    75241 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    75301 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    75361 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    75421 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    75481 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    75541 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    75601 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    75661 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    75721 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    75781 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    75841 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    75901 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    75961 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga attttttttt
    76021 tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg
    76081 ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac
    76141 ctaaaaaaag
//
LOCUS       DAZKAY010000002        64905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000002 DAZKAY010000000
VERSION     DAZKAY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.002"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1005
                     /locus_tag="ACG8WP_000066"
     CDS             142..1005
                     /locus_tag="ACG8WP_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9026411.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1134..2756)
                     /locus_tag="ACG8WP_000067"
     CDS             complement(1134..2756)
                     /locus_tag="ACG8WP_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9026412.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3005..3526
                     /locus_tag="ACG8WP_000068"
     CDS             3005..3526
                     /locus_tag="ACG8WP_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9026413.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG8WP_000069"
     CDS             complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG8WP_000069"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9026414.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5607..6425
                     /locus_tag="ACG8WP_000070"
     CDS             5607..6425
                     /locus_tag="ACG8WP_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9026415.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6528..6725)
                     /locus_tag="ACG8WP_000071"
     CDS             complement(6528..6725)
                     /locus_tag="ACG8WP_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026416.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6894..7472
                     /locus_tag="ACG8WP_000072"
     CDS             6894..7472
                     /locus_tag="ACG8WP_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9026417.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7567..7704
                     /locus_tag="ACG8WP_000073"
     CDS             7567..7704
                     /locus_tag="ACG8WP_000073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026418.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8517..9020)
                     /locus_tag="ACG8WP_000074"
     CDS             complement(8517..9020)
                     /locus_tag="ACG8WP_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9026419.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9379..10404
                     /locus_tag="ACG8WP_000075"
     CDS             9379..10404
                     /locus_tag="ACG8WP_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9026420.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10727..11605
                     /locus_tag="ACG8WP_000076"
     CDS             10727..11605
                     /locus_tag="ACG8WP_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9026421.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12081..12197
                     /locus_tag="ACG8WP_000077"
     CDS             12081..12197
                     /locus_tag="ACG8WP_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9026422.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13125..13463
                     /locus_tag="ACG8WP_000078"
     CDS             13125..13463
                     /locus_tag="ACG8WP_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026423.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13557..13787
                     /locus_tag="ACG8WP_000079"
     CDS             13557..13787
                     /locus_tag="ACG8WP_000079"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9026424.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14187..14558
                     /locus_tag="ACG8WP_000080"
     CDS             14187..14558
                     /locus_tag="ACG8WP_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9026425.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14832..15524)
                     /locus_tag="ACG8WP_000081"
     CDS             complement(14832..15524)
                     /locus_tag="ACG8WP_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9026426.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15527..16312)
                     /locus_tag="ACG8WP_000082"
     CDS             complement(15527..16312)
                     /locus_tag="ACG8WP_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9026427.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16549..17949
                     /gene="lacG"
                     /locus_tag="ACG8WP_000083"
     CDS             16549..17949
                     /gene="lacG"
                     /locus_tag="ACG8WP_000083"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9026428.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17974..18291
                     /locus_tag="ACG8WP_000084"
     CDS             17974..18291
                     /locus_tag="ACG8WP_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9026429.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18304..19977
                     /locus_tag="ACG8WP_000085"
     CDS             18304..19977
                     /locus_tag="ACG8WP_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9026430.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19991..21622
                     /locus_tag="ACG8WP_000086"
     CDS             19991..21622
                     /locus_tag="ACG8WP_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9026431.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22113..23678)
                     /gene="guaA"
                     /locus_tag="ACG8WP_000087"
     CDS             complement(22113..23678)
                     /gene="guaA"
                     /locus_tag="ACG8WP_000087"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9026432.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23737..24660)
                     /gene="coaA"
                     /locus_tag="ACG8WP_000088"
     CDS             complement(23737..24660)
                     /gene="coaA"
                     /locus_tag="ACG8WP_000088"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9026433.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24856..25461
                     /locus_tag="ACG8WP_000089"
     CDS             24856..25461
                     /locus_tag="ACG8WP_000089"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9026434.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25769..26785
                     /locus_tag="ACG8WP_000090"
     CDS             25769..26785
                     /locus_tag="ACG8WP_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026435.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26926..28227
                     /locus_tag="ACG8WP_000091"
     CDS             26926..28227
                     /locus_tag="ACG8WP_000091"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9026436.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28273..28935
                     /gene="deoC"
                     /locus_tag="ACG8WP_000092"
     CDS             28273..28935
                     /gene="deoC"
                     /locus_tag="ACG8WP_000092"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9026437.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28956..29354
                     /locus_tag="ACG8WP_000093"
     CDS             28956..29354
                     /locus_tag="ACG8WP_000093"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9026438.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29420..30502
                     /locus_tag="ACG8WP_000094"
     CDS             29420..30502
                     /locus_tag="ACG8WP_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9026439.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30714..32276
                     /locus_tag="ACG8WP_000095"
     CDS             30714..32276
                     /locus_tag="ACG8WP_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026440.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32269..33402
                     /locus_tag="ACG8WP_000096"
     CDS             32269..33402
                     /locus_tag="ACG8WP_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026441.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33402..34358
                     /locus_tag="ACG8WP_000097"
     CDS             33402..34358
                     /locus_tag="ACG8WP_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026442.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34457..35623
                     /gene="deoB"
                     /locus_tag="ACG8WP_000098"
     CDS             34457..35623
                     /gene="deoB"
                     /locus_tag="ACG8WP_000098"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9026443.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35647..36465
                     /locus_tag="ACG8WP_000099"
     CDS             35647..36465
                     /locus_tag="ACG8WP_000099"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9026444.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36482..37189
                     /gene="deoD"
                     /locus_tag="ACG8WP_000100"
     CDS             36482..37189
                     /gene="deoD"
                     /locus_tag="ACG8WP_000100"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9026445.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37487..38173)
                     /gene="gpmA"
                     /locus_tag="ACG8WP_000101"
     CDS             complement(37487..38173)
                     /gene="gpmA"
                     /locus_tag="ACG8WP_000101"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9026446.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38345..38926)
                     /locus_tag="ACG8WP_000102"
     CDS             complement(38345..38926)
                     /locus_tag="ACG8WP_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9026447.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39059..39742)
                     /gene="rpiA"
                     /locus_tag="ACG8WP_000103"
     CDS             complement(39059..39742)
                     /gene="rpiA"
                     /locus_tag="ACG8WP_000103"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9026448.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40061..40474
                     /gene="rpsL"
                     /locus_tag="ACG8WP_000104"
     CDS             40061..40474
                     /gene="rpsL"
                     /locus_tag="ACG8WP_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9026449.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40558..41028
                     /gene="rpsG"
                     /locus_tag="ACG8WP_000105"
     CDS             40558..41028
                     /gene="rpsG"
                     /locus_tag="ACG8WP_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9026450.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41122..43209
                     /gene="fusA"
                     /locus_tag="ACG8WP_000106"
     CDS             41122..43209
                     /gene="fusA"
                     /locus_tag="ACG8WP_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9026451.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43381..44568
                     /gene="tuf"
                     /locus_tag="ACG8WP_000107"
     CDS             43381..44568
                     /gene="tuf"
                     /locus_tag="ACG8WP_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9026452.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44975..45283
                     /gene="rpsJ"
                     /locus_tag="ACG8WP_000108"
     CDS             44975..45283
                     /gene="rpsJ"
                     /locus_tag="ACG8WP_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9026453.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45314..45943
                     /gene="rplC"
                     /locus_tag="ACG8WP_000109"
     CDS             45314..45943
                     /gene="rplC"
                     /locus_tag="ACG8WP_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9026454.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45971..46594
                     /gene="rplD"
                     /locus_tag="ACG8WP_000110"
     CDS             45971..46594
                     /gene="rplD"
                     /locus_tag="ACG8WP_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9026455.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46594..46884
                     /gene="rplW"
                     /locus_tag="ACG8WP_000111"
     CDS             46594..46884
                     /gene="rplW"
                     /locus_tag="ACG8WP_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9026456.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46923..47756
                     /gene="rplB"
                     /locus_tag="ACG8WP_000112"
     CDS             46923..47756
                     /gene="rplB"
                     /locus_tag="ACG8WP_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9026457.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47799..48077
                     /gene="rpsS"
                     /locus_tag="ACG8WP_000113"
     CDS             47799..48077
                     /gene="rpsS"
                     /locus_tag="ACG8WP_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9026458.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48100..48447
                     /gene="rplV"
                     /locus_tag="ACG8WP_000114"
     CDS             48100..48447
                     /gene="rplV"
                     /locus_tag="ACG8WP_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9026459.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48461..49117
                     /gene="rpsC"
                     /locus_tag="ACG8WP_000115"
     CDS             48461..49117
                     /gene="rpsC"
                     /locus_tag="ACG8WP_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9026460.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49120..49554
                     /gene="rplP"
                     /locus_tag="ACG8WP_000116"
     CDS             49120..49554
                     /gene="rplP"
                     /locus_tag="ACG8WP_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9026461.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49544..49732
                     /gene="rpmC"
                     /locus_tag="ACG8WP_000117"
     CDS             49544..49732
                     /gene="rpmC"
                     /locus_tag="ACG8WP_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9026462.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49757..50023
                     /gene="rpsQ"
                     /locus_tag="ACG8WP_000118"
     CDS             49757..50023
                     /gene="rpsQ"
                     /locus_tag="ACG8WP_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9026463.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50081..50449
                     /gene="rplN"
                     /locus_tag="ACG8WP_000119"
     CDS             50081..50449
                     /gene="rplN"
                     /locus_tag="ACG8WP_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9026464.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50486..50794
                     /gene="rplX"
                     /locus_tag="ACG8WP_000120"
     CDS             50486..50794
                     /gene="rplX"
                     /locus_tag="ACG8WP_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9026465.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50822..51361
                     /gene="rplE"
                     /locus_tag="ACG8WP_000121"
     CDS             50822..51361
                     /gene="rplE"
                     /locus_tag="ACG8WP_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9026466.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51380..51565
                     /locus_tag="ACG8WP_000122"
     CDS             51380..51565
                     /locus_tag="ACG8WP_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9026467.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51601..51999
                     /gene="rpsH"
                     /locus_tag="ACG8WP_000123"
     CDS             51601..51999
                     /gene="rpsH"
                     /locus_tag="ACG8WP_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9026468.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52031..52567
                     /gene="rplF"
                     /locus_tag="ACG8WP_000124"
     CDS             52031..52567
                     /gene="rplF"
                     /locus_tag="ACG8WP_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9026469.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52728..53084
                     /gene="rplR"
                     /locus_tag="ACG8WP_000125"
     CDS             52728..53084
                     /gene="rplR"
                     /locus_tag="ACG8WP_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9026470.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53105..53605
                     /gene="rpsE"
                     /locus_tag="ACG8WP_000126"
     CDS             53105..53605
                     /gene="rpsE"
                     /locus_tag="ACG8WP_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9026471.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53620..53799
                     /gene="rpmD"
                     /locus_tag="ACG8WP_000127"
     CDS             53620..53799
                     /gene="rpmD"
                     /locus_tag="ACG8WP_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9026472.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53843..54283
                     /gene="rplO"
                     /locus_tag="ACG8WP_000128"
     CDS             53843..54283
                     /gene="rplO"
                     /locus_tag="ACG8WP_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9026473.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54283..55578
                     /gene="secY"
                     /locus_tag="ACG8WP_000129"
     CDS             54283..55578
                     /gene="secY"
                     /locus_tag="ACG8WP_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9026474.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55640..56287
                     /locus_tag="ACG8WP_000130"
     CDS             55640..56287
                     /locus_tag="ACG8WP_000130"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9026475.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56478..56696
                     /gene="infA"
                     /locus_tag="ACG8WP_000131"
     CDS             56478..56696
                     /gene="infA"
                     /locus_tag="ACG8WP_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9026476.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56729..56845
                     /gene="rpmJ"
                     /locus_tag="ACG8WP_000132"
     CDS             56729..56845
                     /gene="rpmJ"
                     /locus_tag="ACG8WP_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9026477.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56863..57228
                     /gene="rpsM"
                     /locus_tag="ACG8WP_000133"
     CDS             56863..57228
                     /gene="rpsM"
                     /locus_tag="ACG8WP_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9026478.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57256..57645
                     /gene="rpsK"
                     /locus_tag="ACG8WP_000134"
     CDS             57256..57645
                     /gene="rpsK"
                     /locus_tag="ACG8WP_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9026479.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57725..58663
                     /locus_tag="ACG8WP_000135"
     CDS             57725..58663
                     /locus_tag="ACG8WP_000135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9026480.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58691..59071
                     /gene="rplQ"
                     /locus_tag="ACG8WP_000136"
     CDS             58691..59071
                     /gene="rplQ"
                     /locus_tag="ACG8WP_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9026481.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59343..60182
                     /locus_tag="ACG8WP_000137"
     CDS             59343..60182
                     /locus_tag="ACG8WP_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9026482.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60158..61027
                     /locus_tag="ACG8WP_000138"
     CDS             60158..61027
                     /locus_tag="ACG8WP_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9026483.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61017..61814
                     /locus_tag="ACG8WP_000139"
     CDS             61017..61814
                     /locus_tag="ACG8WP_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9026484.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62017..62763
                     /gene="truA"
                     /locus_tag="ACG8WP_000140"
     CDS             62017..62763
                     /gene="truA"
                     /locus_tag="ACG8WP_000140"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9026485.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63427..63621
                     /locus_tag="ACG8WP_000141"
                     /pseudo
     CDS             63427..63621
                     /locus_tag="ACG8WP_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(63809..64585)
                     /locus_tag="ACG8WP_000142"
     CDS             complement(63809..64585)
                     /locus_tag="ACG8WP_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9026486.1"
                     /translation="MNIHHIRNATAILDYGSKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            64708..>64905
                     /locus_tag="ACG8WP_000143"
     CDS             64708..>64905
                     /locus_tag="ACG8WP_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFP9026487.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHA"
BASE COUNT        20596 a        11577 c        13730 g        19002 t
ORIGIN      
        1 ttcttgaagc tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa
       61 accggttaaa gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt
      121 aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca
      181 taccatgtat tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat
      241 caagagttag tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg
      301 aaaaatggtg cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc
      361 cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc
      421 ggctcttaca gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct
      481 gagatctatg tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa
      541 gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt
      601 aattatttga aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc
      661 ggtaggattt ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt
      721 acggtggata aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg
      781 atccagacaa tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt
      841 tatgtggctt acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag
      901 cagataaaga tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca
      961 ggctatggct gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt
     1021 ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag
     1081 catcctatct cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc
     1141 cataactcgt tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta
     1201 tcagctcgat cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg
     1261 ataaattgat ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca
     1321 ttcaatgctg tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg
     1381 ttagctttcg aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa
     1441 acattcactg gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt
     1501 aagaaggtgt tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt
     1561 agatcagtat cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca
     1621 ccccaacgta ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt
     1681 gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa
     1741 gatatatcat ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat
     1801 aagtcgtttc caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa
     1861 ggttggatat cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt
     1921 gattttgatg cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct
     1981 tcttttttgg catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa
     2041 aaatcataat ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta
     2101 catactttgt tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg
     2161 atcaaaaatt cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct
     2221 agcaaaagaa catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt
     2281 ccaccagtta attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc
     2341 agtaaaacag ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct
     2401 aattcagctg ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct
     2461 ttttctttca gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta
     2521 tagtcttcat agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata
     2581 gtaacggaac ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta
     2641 cctgcaccat ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca
     2701 tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt
     2761 tttcctcact tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg
     2821 taaaagattc aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat
     2881 aaaaataaaa gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat
     2941 ttttgacgtt atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt
     3001 gaaaatggat gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt
     3061 acttgtctca ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt
     3121 ctttgcgatt tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa
     3181 agagctaaca ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt
     3241 tggcgtcttt ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc
     3301 agtatggttt attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt
     3361 aagtgaagga tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat
     3421 tttcttattg tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta
     3481 ttttatgtta tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa
     3541 ataaaaaagc cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat
     3601 catggacaaa tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg
     3661 agaagctata tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct
     3721 cttttcatat aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc
     3781 ttggatttct tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg
     3841 gcgcttgtta ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc
     3901 aaaggcaaat cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc
     3961 tccaacttta ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc
     4021 ttcttgttct gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg
     4081 aagctgatat ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc
     4141 acttaaggta aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac
     4201 accattaccc atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt
     4261 caataaacga atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc
     4321 ttcagcaata cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg
     4381 atcttcaaaa gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta
     4441 ccagctatca gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc
     4501 atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc
     4561 gatttggata aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc
     4621 gacaaataag atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg
     4681 tccagcaaaa ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga
     4741 agcgacaaca ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg
     4801 cgttccttga ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc
     4861 tagcaaatct ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa
     4921 aggttgttcg tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc
     4981 tttatgctca taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt
     5041 catgactgta cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg
     5101 gaaagagtta tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac
     5161 atggtccatc tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga
     5221 gccgaagaca gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc
     5281 gacttcttca tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc
     5341 cataattgaa ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc
     5401 aagaactttt accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag
     5461 caatgatgat acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa
     5521 ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat
     5581 agatagattc aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc
     5641 agcaagcact tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg
     5701 tttttttctt taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg
     5761 gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag
     5821 aaaatggaga tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt
     5881 tatatatggc agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag
     5941 gattgcctac ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca
     6001 ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga
     6061 cagccgctat attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag
     6121 aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg
     6181 tttacggaca agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag
     6241 taacgaaagt ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt
     6301 atgtcgtggt ggaagaattg atccctgaag cacagcagac attgtccagc aaacatcatt
     6361 ttgcagtttt tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag
     6421 gataaataaa aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc
     6481 tattttttta gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt
     6541 tttctatgtg tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt
     6601 agaactttga ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg
     6661 aagctagctg caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga
     6721 cgcatacaaa accctcctct tgttcattct ttactataga aaaagtataa cagggattgt
     6781 ttaaaaacac ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat
     6841 tgctttttct ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta
     6901 aaaatcgtct tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt
     6961 tagaacttca tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat
     7021 tttttatcta tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt
     7081 tatttcaagt tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt
     7141 ttgcgctttc gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg
     7201 ctccagaaat gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg
     7261 caaaagaact tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata
     7321 attacaagaa atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt
     7381 tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc
     7441 caagtcgtac catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa
     7501 aaatatagaa aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg
     7561 atgcagatgg atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt
     7621 atttatttgg atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca
     7681 ttattcacag caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag
     7741 aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt
     7801 aatttttaga aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg
     7861 taagagataa aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga
     7921 tatacaacag aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt
     7981 ttggaataaa aaaataacat atctcatttt acacttttgt tattttatac ttaaaaaata
     8041 acgacacaga aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt
     8101 tcctttgaaa tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga
     8161 agggagccta aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag
     8221 ctttttatag aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta
     8281 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
     8341 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
     8401 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
     8461 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
     8521 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
     8581 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
     8641 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
     8701 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
     8761 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
     8821 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
     8881 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
     8941 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
     9001 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
     9061 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
     9121 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
     9181 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
     9241 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     9301 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     9361 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     9421 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     9481 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     9541 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     9601 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     9661 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     9721 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     9781 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     9841 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     9901 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     9961 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
    10021 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
    10081 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
    10141 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
    10201 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
    10261 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
    10321 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
    10381 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
    10441 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
    10501 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
    10561 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
    10621 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
    10681 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
    10741 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
    10801 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
    10861 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
    10921 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
    10981 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
    11041 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
    11101 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
    11161 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
    11221 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
    11281 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
    11341 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
    11401 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
    11461 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
    11521 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
    11581 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
    11641 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    11701 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
    11761 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga
    11821 acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca
    11881 agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag
    11941 tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac
    12001 catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa
    12061 gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga
    12121 aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga
    12181 atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt
    12241 cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc
    12301 catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa
    12361 ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct
    12421 gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta
    12481 ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac
    12541 ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg
    12601 ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt
    12661 cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc
    12721 gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt
    12781 actattgcta gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa
    12841 gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat
    12901 cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta
    12961 aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt
    13021 tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga
    13081 ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt
    13141 caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa
    13201 atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa
    13261 taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata
    13321 cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata
    13381 ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata
    13441 aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga
    13501 aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc
    13561 tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg
    13621 aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag
    13681 tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt
    13741 tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt
    13801 atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta
    13861 ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta
    13921 gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt
    13981 ttcttaatta gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt
    14041 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
    14101 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
    14161 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
    14221 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
    14281 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
    14341 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
    14401 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
    14461 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
    14521 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
    14581 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
    14641 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
    14701 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
    14761 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
    14821 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
    14881 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
    14941 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
    15001 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
    15061 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
    15121 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
    15181 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
    15241 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
    15301 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
    15361 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
    15421 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
    15481 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
    15541 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
    15601 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
    15661 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
    15721 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
    15781 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
    15841 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
    15901 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
    15961 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
    16021 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
    16081 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
    16141 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
    16201 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
    16261 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
    16321 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
    16381 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
    16441 ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
    16501 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
    16561 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
    16621 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
    16681 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
    16741 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
    16801 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
    16861 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
    16921 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
    16981 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
    17041 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
    17101 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
    17161 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
    17221 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
    17281 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
    17341 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
    17401 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
    17461 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
    17521 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
    17581 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
    17641 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
    17701 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
    17761 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
    17821 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
    17881 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
    17941 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
    18001 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
    18061 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
    18121 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
    18181 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
    18241 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
    18301 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
    18361 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
    18421 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
    18481 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
    18541 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
    18601 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
    18661 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
    18721 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
    18781 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
    18841 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
    18901 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
    18961 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
    19021 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
    19081 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
    19141 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
    19201 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
    19261 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
    19321 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
    19381 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
    19441 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
    19501 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
    19561 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
    19621 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
    19681 gtactttgtg ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca
    19741 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
    19801 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
    19861 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
    19921 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
    19981 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
    20041 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
    20101 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
    20161 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
    20221 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
    20281 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
    20341 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
    20401 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
    20461 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
    20521 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
    20581 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
    20641 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
    20701 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
    20761 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
    20821 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
    20881 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
    20941 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
    21001 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
    21061 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
    21121 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
    21181 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
    21241 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
    21301 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
    21361 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
    21421 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
    21481 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
    21541 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
    21601 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
    21661 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
    21721 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
    21781 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
    21841 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
    21901 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
    21961 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
    22021 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
    22081 tagggattcc ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt
    22141 ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca
    22201 gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt
    22261 gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg
    22321 acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca
    22381 gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca
    22441 gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg
    22501 attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg
    22561 tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga
    22621 gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct
    22681 tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca
    22741 tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac
    22801 ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct
    22861 agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata
    22921 gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg
    22981 ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact
    23041 tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca
    23101 aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata
    23161 ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg
    23221 actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt
    23281 tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct
    23341 ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat
    23401 tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc
    23461 ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga
    23521 ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca
    23581 agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata
    23641 atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta
    23701 aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga
    23761 atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa
    23821 gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa
    23881 ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc
    23941 cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc
    24001 atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa
    24061 ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt
    24121 gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc
    24181 agattttaca cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc
    24241 tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt
    24301 cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct
    24361 ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac
    24421 ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat
    24481 ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat
    24541 gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt
    24601 acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat
    24661 attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc
    24721 caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag
    24781 tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg
    24841 agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat
    24901 tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt
    24961 gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg
    25021 gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg
    25081 ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg
    25141 gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa
    25201 tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg
    25261 atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag
    25321 aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag
    25381 aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc
    25441 atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg
    25501 ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag
    25561 aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat
    25621 aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat
    25681 caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt acactgatag
    25741 ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat
    25801 ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag
    25861 tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta
    25921 tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt
    25981 cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg
    26041 ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca
    26101 agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga
    26161 gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg
    26221 attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc
    26281 tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg
    26341 atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct
    26401 gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc
    26461 atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat
    26521 ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc
    26581 agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt
    26641 gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga
    26701 atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag
    26761 cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa
    26821 ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc
    26881 taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat
    26941 ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc
    27001 acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt
    27061 ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct
    27121 ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt
    27181 ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca
    27241 gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa
    27301 gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa
    27361 agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat
    27421 gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg
    27481 agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt
    27541 gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg
    27601 aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa
    27661 gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca
    27721 gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa
    27781 gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg
    27841 ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat
    27901 ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt
    27961 gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt
    28021 aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt
    28081 gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact
    28141 tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg
    28201 ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa
    28261 aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca
    28321 tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc
    28381 tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc
    28441 gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa
    28501 acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct
    28561 ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca
    28621 aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc
    28681 atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt
    28741 ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca
    28801 gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc
    28861 gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact
    28921 ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga
    28981 tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag
    29041 gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat
    29101 catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag
    29161 aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg
    29221 gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag
    29281 gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg
    29341 aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat
    29401 tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact
    29461 agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg
    29521 caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga
    29581 ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact
    29641 ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc
    29701 aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg gttataaact
    29761 aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga
    29821 tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc
    29881 ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg
    29941 cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc
    30001 aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc
    30061 tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat
    30121 cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga
    30181 atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga
    30241 atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg
    30301 aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca
    30361 cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga
    30421 agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc
    30481 agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa
    30541 ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg
    30601 gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt
    30661 gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc
    30721 aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa
    30781 atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg
    30841 gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga
    30901 aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag
    30961 ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca
    31021 tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag
    31081 agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg
    31141 atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag
    31201 atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa
    31261 tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt
    31321 tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg
    31381 gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa
    31441 cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg
    31501 aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg
    31561 ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga
    31621 tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag
    31681 acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag
    31741 accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa
    31801 cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac
    31861 atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa
    31921 aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc
    31981 ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta
    32041 ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg
    32101 aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg
    32161 tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct
    32221 ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc
    32281 agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta
    32341 ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta
    32401 gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg
    32461 atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt
    32521 tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat
    32581 ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg
    32641 gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca
    32701 atcatgatga actatatctt attatacgta agcacatttg ctttacatga agtgatgcct
    32761 gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg
    32821 ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca
    32881 atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc
    32941 cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg
    33001 atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga
    33061 ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga
    33121 atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg
    33181 ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag
    33241 atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt
    33301 ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa
    33361 tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa
    33421 ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta acgtcactag
    33481 gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca
    33541 tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga
    33601 caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg
    33661 cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga
    33721 tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata
    33781 acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg
    33841 gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc
    33901 tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa
    33961 atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac
    34021 tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta
    34081 acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg
    34141 gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct
    34201 tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa
    34261 ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc
    34321 caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt
    34381 gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa
    34441 gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat
    34501 tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat
    34561 tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg
    34621 tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga
    34681 agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa
    34741 aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga
    34801 attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga
    34861 ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt
    34921 ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca
    34981 attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc
    35041 gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa
    35101 tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt
    35161 tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag
    35221 caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag
    35281 ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta
    35341 tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga
    35401 agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga
    35461 tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc
    35521 attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc
    35581 ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga
    35641 aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa
    35701 ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat
    35761 gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca
    35821 gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc
    35881 atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta
    35941 cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc
    36001 aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt
    36061 gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt
    36121 caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg
    36181 gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag
    36241 atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc
    36301 atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca
    36361 gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa
    36421 caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa
    36481 aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg
    36541 agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa
    36601 tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca
    36661 aggaacagga atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta
    36721 tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt
    36781 tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt
    36841 ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat
    36901 agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta
    36961 caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat
    37021 ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat
    37081 gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac
    37141 gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga
    37201 aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt
    37261 gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg
    37321 tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    37381 tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa
    37441 agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt
    37501 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
    37561 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
    37621 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
    37681 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
    37741 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
    37801 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
    37861 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
    37921 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
    37981 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
    38041 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
    38101 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
    38161 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
    38221 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
    38281 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
    38341 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
    38401 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
    38461 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
    38521 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
    38581 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
    38641 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
    38701 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
    38761 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
    38821 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
    38881 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
    38941 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
    39001 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
    39061 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
    39121 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
    39181 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
    39241 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
    39301 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
    39361 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
    39421 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
    39481 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
    39541 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
    39601 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
    39661 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
    39721 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
    39781 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
    39841 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
    39901 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
    39961 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
    40021 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
    40081 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
    40141 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
    40201 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
    40261 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
    40321 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
    40381 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
    40441 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
    40501 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
    40561 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
    40621 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
    40681 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
    40741 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
    40801 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
    40861 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
    40921 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
    40981 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
    41041 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
    41101 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
    41161 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
    41221 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
    41281 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
    41341 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
    41401 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
    41461 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
    41521 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
    41581 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
    41641 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
    41701 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
    41761 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
    41821 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
    41881 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
    41941 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
    42001 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
    42061 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
    42121 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
    42181 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
    42241 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
    42301 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
    42361 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
    42421 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
    42481 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
    42541 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
    42601 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
    42661 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
    42721 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
    42781 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
    42841 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
    42901 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
    42961 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
    43021 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
    43081 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
    43141 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
    43201 gctgaataat ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
    43261 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
    43321 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
    43381 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
    43441 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
    43501 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
    43561 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
    43621 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
    43681 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
    43741 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
    43801 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
    43861 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
    43921 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
    43981 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
    44041 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
    44101 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
    44161 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
    44221 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
    44281 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
    44341 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    44401 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
    44461 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
    44521 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
    44581 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
    44641 gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga
    44701 agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt
    44761 ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt
    44821 tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt
    44881 tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg
    44941 tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg
    45001 tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc
    45061 aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta
    45121 cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac
    45181 acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa
    45241 gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa
    45301 tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt
    45361 cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact
    45421 acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat
    45481 gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc
    45541 taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga
    45601 aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg
    45661 taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg
    45721 ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa
    45781 aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt
    45841 tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa
    45901 aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag
    45961 gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat
    46021 cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat
    46081 cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt
    46141 tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc
    46201 tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta
    46261 cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa
    46321 agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa
    46381 agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga
    46441 aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac
    46501 ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac
    46561 tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc
    46621 ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg
    46681 gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa
    46741 gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca
    46801 ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc
    46861 caattattcg aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa
    46921 acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg
    46981 attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc
    47041 atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac
    47101 gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa
    47161 cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag
    47221 tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc
    47281 cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg
    47341 ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta
    47401 caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg
    47461 aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac
    47521 acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg
    47581 tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc
    47641 ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc
    47701 gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac
    47761 tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag
    47821 gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga
    47881 aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata
    47941 ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag
    48001 gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata
    48061 agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca
    48121 gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac
    48181 cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa
    48241 tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt
    48301 gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg
    48361 aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat
    48421 atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc
    48481 aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga
    48541 gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc
    48601 tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat
    48661 tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag
    48721 aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa
    48781 accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt
    48841 tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg
    48901 gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata
    48961 ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga
    49021 agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct
    49081 tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta
    49141 aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta
    49201 gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc
    49261 gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa
    49321 attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa
    49381 ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca
    49441 ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg
    49501 aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat
    49561 cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt
    49621 taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt
    49681 acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa
    49741 ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt
    49801 ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc
    49861 tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac
    49921 agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg
    49981 tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct
    50041 atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg
    50101 tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg
    50161 atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac
    50221 gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc
    50281 aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat
    50341 tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg
    50401 tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac
    50461 ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat
    50521 cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa
    50581 agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc
    50641 tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga
    50701 ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt
    50761 ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg
    50821 aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt
    50881 taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt
    50941 tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat
    51001 cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg
    51061 tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt
    51121 agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa
    51181 agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga
    51241 agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc
    51301 aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata
    51361 attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc
    51421 aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta
    51481 tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc
    51541 cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca
    51601 atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt
    51661 aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg
    51721 aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc
    51781 cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc
    51841 tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt
    51901 ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa
    51961 aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag
    52021 gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt
    52081 tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt
    52141 ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga
    52201 cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt
    52261 aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca
    52321 attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc
    52381 accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa
    52441 caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta
    52501 taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg
    52561 taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga
    52621 agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta
    52681 tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac
    52741 cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta
    52801 ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta
    52861 ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag
    52921 gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg
    52981 aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc
    53041 aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc
    53101 attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa
    53161 ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt
    53221 cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc
    53281 aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc
    53341 aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc
    53401 tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg
    53461 ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc
    53521 aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa
    53581 atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact
    53641 ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt
    53701 cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc
    53761 aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga
    53821 acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg
    53881 ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg
    53941 cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg
    54001 acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga
    54061 ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc
    54121 tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc
    54181 taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca
    54241 agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa
    54301 gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt
    54361 gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac
    54421 ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac
    54481 ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt
    54541 cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt
    54601 aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc
    54661 ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta
    54721 gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta
    54781 ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt
    54841 atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt
    54901 gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg
    54961 ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat
    55021 acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct
    55081 ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa
    55141 gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac
    55201 tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc
    55261 tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt
    55321 tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg
    55381 cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct
    55441 caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt
    55501 atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat
    55561 gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca
    55621 tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg
    55681 aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat
    55741 gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga
    55801 taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga
    55861 accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa
    55921 agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca
    55981 tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg
    56041 agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg
    56101 tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc
    56161 tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa
    56221 cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga
    56281 aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat
    56341 aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga
    56401 gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt
    56461 acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg
    56521 aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta
    56581 ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg
    56641 ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt
    56701 actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg
    56761 cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc
    56821 aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga
    56881 gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt
    56941 aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt
    57001 gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa
    57061 ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac
    57121 cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca
    57181 cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa
    57241 ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat
    57301 atagaagcag gtattgcaca tatccattct acattcaata acactatcat catgatcact
    57361 gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc
    57421 aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg
    57481 gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca
    57541 gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca
    57601 gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt
    57661 ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat
    57721 aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg
    57781 caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg
    57841 tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt
    57901 actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa
    57961 cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat
    58021 taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct
    58081 taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac
    58141 agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat
    58201 tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga
    58261 gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga
    58321 tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt
    58381 ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga
    58441 agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg
    58501 ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc
    58561 tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc
    58621 aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag
    58681 ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt
    58741 gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc
    58801 gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca
    58861 tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa
    58921 tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta
    58981 cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg
    59041 tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat
    59101 gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct
    59161 cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc
    59221 aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa
    59281 ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa
    59341 tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc
    59401 gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg
    59461 gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag
    59521 aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc
    59581 gacagatggt tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag
    59641 aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc
    59701 gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc
    59761 gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg
    59821 atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga
    59881 tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg
    59941 atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc
    60001 gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag
    60061 atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca
    60121 gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt
    60181 agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat
    60241 gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac
    60301 cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga
    60361 acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg
    60421 catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc
    60481 tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac
    60541 attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg
    60601 agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt
    60661 attggatgaa ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt
    60721 ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga
    60781 tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg
    60841 ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc
    60901 tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc
    60961 tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga
    61021 tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc
    61081 cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt
    61141 gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag
    61201 atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag
    61261 ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata
    61321 tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt
    61381 ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt
    61441 acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt
    61501 cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc
    61561 agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca
    61621 ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg
    61681 ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt
    61741 ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct
    61801 ttttgagagg ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc
    61861 aagaaaggat gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca
    61921 caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat
    61981 tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt
    62041 gcatacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa
    62101 gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt
    62161 tcgggaagga cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca
    62221 gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt
    62281 gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa
    62341 aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat
    62401 gcaagttatt ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta
    62461 ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta
    62521 cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca
    62581 tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa
    62641 attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt
    62701 aatgcagcag gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa
    62761 taagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac
    62821 ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg
    62881 aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata
    62941 gctcgtcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    63001 tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa
    63061 aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac
    63121 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa
    63181 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    63241 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    63301 cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt
    63361 tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact
    63421 acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa
    63481 aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc
    63541 aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat
    63601 ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa
    63661 aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat
    63721 tcttaaagta cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct
    63781 atccctttat taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca
    63841 ctaaaacttt ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca
    63901 gtccccaatg gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt
    63961 tatgcaattc atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat
    64021 tatctccagc atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag
    64081 cttcatacca aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa
    64141 caataccgca aacattacct acataattca agatttctcc tctgccatgt ttgccttttg
    64201 ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa
    64261 aaccatatag tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt
    64321 taggtaggac ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa
    64381 cagcatcaat accgttgata atatcttcga ctggcattgg caaatcaact agaggattca
    64441 tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt
    64501 tttccgctaa cattggatca attaaaaatt ttttacttcc atagtctaag atagctgttg
    64561 cattacgaat gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt
    64621 ttagtataat tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt
    64681 tacttaattt atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag
    64741 gaactatcaa aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact
    64801 ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat
    64861 acagtaaaat taaatatgga aaagctaggg ttttcgattc acgct
//
LOCUS       DAZKAY010000003        61853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000003 DAZKAY010000000
VERSION     DAZKAY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.003"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..608
                     /locus_tag="ACG8WP_000144"
     CDS             90..608
                     /locus_tag="ACG8WP_000144"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9026488.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            736..1419
                     /locus_tag="ACG8WP_000145"
     CDS             736..1419
                     /locus_tag="ACG8WP_000145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9026489.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1728..1801
                     /locus_tag="ACG8WP_000146"
     tRNA            1728..1801
                     /locus_tag="ACG8WP_000146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1762..1764,aa:Arg,seq:tct)
     gene            1960..2319
                     /locus_tag="ACG8WP_000147"
     CDS             1960..2319
                     /locus_tag="ACG8WP_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9026490.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2442..4073
                     /locus_tag="ACG8WP_000148"
     CDS             2442..4073
                     /locus_tag="ACG8WP_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9026491.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4135..4776)
                     /locus_tag="ACG8WP_000149"
     CDS             complement(4135..4776)
                     /locus_tag="ACG8WP_000149"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9026492.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4962..5038
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5058..5366
                     /gene="rplU"
                     /locus_tag="ACG8WP_000150"
     CDS             5058..5366
                     /gene="rplU"
                     /locus_tag="ACG8WP_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9026493.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5393..5737
                     /locus_tag="ACG8WP_000151"
     CDS             5393..5737
                     /locus_tag="ACG8WP_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9026494.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5750..6043
                     /gene="rpmA"
                     /locus_tag="ACG8WP_000152"
     CDS             5750..6043
                     /gene="rpmA"
                     /locus_tag="ACG8WP_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9026495.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6477..9284
                     /locus_tag="ACG8WP_000153"
     CDS             6477..9284
                     /locus_tag="ACG8WP_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9026496.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9475..9966
                     /locus_tag="ACG8WP_000154"
     CDS             9475..9966
                     /locus_tag="ACG8WP_000154"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9026497.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10069..11058
                     /locus_tag="ACG8WP_000155"
     CDS             10069..11058
                     /locus_tag="ACG8WP_000155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9026498.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11091..11894
                     /locus_tag="ACG8WP_000156"
     CDS             11091..11894
                     /locus_tag="ACG8WP_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9026499.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11915..12829
                     /locus_tag="ACG8WP_000157"
     CDS             11915..12829
                     /locus_tag="ACG8WP_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9026500.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12912..13286
                     /locus_tag="ACG8WP_000158"
     CDS             12912..13286
                     /locus_tag="ACG8WP_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9026501.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13425..13670
                     /locus_tag="ACG8WP_000159"
     CDS             13425..13670
                     /locus_tag="ACG8WP_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9026502.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13842..14906
                     /locus_tag="ACG8WP_000160"
     CDS             13842..14906
                     /locus_tag="ACG8WP_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9026503.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15152..15577
                     /locus_tag="ACG8WP_000161"
     CDS             15152..15577
                     /locus_tag="ACG8WP_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9026504.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15570..16019
                     /gene="nusB"
                     /locus_tag="ACG8WP_000162"
     CDS             15570..16019
                     /gene="nusB"
                     /locus_tag="ACG8WP_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9026505.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16116..16970
                     /locus_tag="ACG8WP_000163"
     CDS             16116..16970
                     /locus_tag="ACG8WP_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9026506.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16971..18308
                     /gene="xseA"
                     /locus_tag="ACG8WP_000164"
     CDS             16971..18308
                     /gene="xseA"
                     /locus_tag="ACG8WP_000164"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9026507.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18313..18537
                     /locus_tag="ACG8WP_000165"
     CDS             18313..18537
                     /locus_tag="ACG8WP_000165"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9026508.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18539..19429
                     /locus_tag="ACG8WP_000166"
     CDS             18539..19429
                     /locus_tag="ACG8WP_000166"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9026509.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19438..20256
                     /locus_tag="ACG8WP_000167"
     CDS             19438..20256
                     /locus_tag="ACG8WP_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9026510.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20363..20812
                     /locus_tag="ACG8WP_000168"
     CDS             20363..20812
                     /locus_tag="ACG8WP_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9026511.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20830..22512
                     /gene="recN"
                     /locus_tag="ACG8WP_000169"
     CDS             20830..22512
                     /gene="recN"
                     /locus_tag="ACG8WP_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9026512.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22567..22683)
                     /locus_tag="ACG8WP_000170"
     CDS             complement(22567..22683)
                     /locus_tag="ACG8WP_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9026513.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22756..23712)
                     /locus_tag="ACG8WP_000171"
     CDS             complement(22756..23712)
                     /locus_tag="ACG8WP_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9026514.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23883..24251
                     /locus_tag="ACG8WP_000172"
     CDS             23883..24251
                     /locus_tag="ACG8WP_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9026515.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24421..24852
                     /gene="mraZ"
                     /locus_tag="ACG8WP_000173"
     CDS             24421..24852
                     /gene="mraZ"
                     /locus_tag="ACG8WP_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9026516.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24873..25832
                     /gene="rsmH"
                     /locus_tag="ACG8WP_000174"
     CDS             24873..25832
                     /gene="rsmH"
                     /locus_tag="ACG8WP_000174"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9026517.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25837..26238
                     /gene="ftsL"
                     /locus_tag="ACG8WP_000175"
     CDS             25837..26238
                     /gene="ftsL"
                     /locus_tag="ACG8WP_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9026518.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26239..28431
                     /locus_tag="ACG8WP_000176"
     CDS             26239..28431
                     /locus_tag="ACG8WP_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9026519.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28457..29419
                     /gene="mraY"
                     /locus_tag="ACG8WP_000177"
     CDS             28457..29419
                     /gene="mraY"
                     /locus_tag="ACG8WP_000177"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9026520.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29433..30794
                     /gene="murD"
                     /locus_tag="ACG8WP_000178"
     CDS             29433..30794
                     /gene="murD"
                     /locus_tag="ACG8WP_000178"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9026521.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30820..31908
                     /gene="murG"
                     /locus_tag="ACG8WP_000179"
     CDS             30820..31908
                     /gene="murG"
                     /locus_tag="ACG8WP_000179"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9026522.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31923..33143
                     /locus_tag="ACG8WP_000180"
     CDS             31923..33143
                     /locus_tag="ACG8WP_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9026523.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33299..34624
                     /gene="ftsA"
                     /locus_tag="ACG8WP_000181"
     CDS             33299..34624
                     /gene="ftsA"
                     /locus_tag="ACG8WP_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9026524.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34647..35888
                     /gene="ftsZ"
                     /locus_tag="ACG8WP_000182"
     CDS             34647..35888
                     /gene="ftsZ"
                     /locus_tag="ACG8WP_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9026525.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35905..36582
                     /locus_tag="ACG8WP_000183"
     CDS             35905..36582
                     /locus_tag="ACG8WP_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9026526.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36596..37195
                     /locus_tag="ACG8WP_000184"
     CDS             36596..37195
                     /locus_tag="ACG8WP_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9026527.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37276..37497
                     /locus_tag="ACG8WP_000185"
     CDS             37276..37497
                     /locus_tag="ACG8WP_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9026528.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37546..38328
                     /locus_tag="ACG8WP_000186"
     CDS             37546..38328
                     /locus_tag="ACG8WP_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9026529.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38349..39056
                     /locus_tag="ACG8WP_000187"
     CDS             38349..39056
                     /locus_tag="ACG8WP_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9026530.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39318..42116
                     /gene="ileS"
                     /locus_tag="ACG8WP_000188"
     CDS             39318..42116
                     /gene="ileS"
                     /locus_tag="ACG8WP_000188"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9026531.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42165..43691)
                     /gene="zwf"
                     /locus_tag="ACG8WP_000189"
     CDS             complement(42165..43691)
                     /gene="zwf"
                     /locus_tag="ACG8WP_000189"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9026532.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43706..44353)
                     /locus_tag="ACG8WP_000190"
     CDS             complement(43706..44353)
                     /locus_tag="ACG8WP_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9026533.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44537..44866)
                     /locus_tag="ACG8WP_000191"
     CDS             complement(44537..44866)
                     /locus_tag="ACG8WP_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9026534.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45043..45771
                     /gene="sfsA"
                     /locus_tag="ACG8WP_000192"
     CDS             45043..45771
                     /gene="sfsA"
                     /locus_tag="ACG8WP_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9026535.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45787..46800
                     /locus_tag="ACG8WP_000193"
     CDS             45787..46800
                     /locus_tag="ACG8WP_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9026536.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46800..48077
                     /locus_tag="ACG8WP_000194"
     CDS             46800..48077
                     /locus_tag="ACG8WP_000194"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9026537.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48140..50842
                     /locus_tag="ACG8WP_000195"
     CDS             48140..50842
                     /locus_tag="ACG8WP_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9026538.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50994..51311
                     /locus_tag="ACG8WP_000196"
     CDS             50994..51311
                     /locus_tag="ACG8WP_000196"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9026539.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51341..51661
                     /locus_tag="ACG8WP_000197"
     CDS             51341..51661
                     /locus_tag="ACG8WP_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9026540.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51769..53229
                     /locus_tag="ACG8WP_000198"
     CDS             51769..53229
                     /locus_tag="ACG8WP_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9026541.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53297..53518
                     /locus_tag="ACG8WP_000199"
     CDS             53297..53518
                     /locus_tag="ACG8WP_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026542.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53549..53731
                     /locus_tag="ACG8WP_000200"
     CDS             53549..53731
                     /locus_tag="ACG8WP_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9026543.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53731..54144
                     /locus_tag="ACG8WP_000201"
     CDS             53731..54144
                     /locus_tag="ACG8WP_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9026544.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54267..55448
                     /locus_tag="ACG8WP_000202"
     CDS             54267..55448
                     /locus_tag="ACG8WP_000202"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9026545.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55519..55683)
                     /locus_tag="ACG8WP_000203"
     CDS             complement(55519..55683)
                     /locus_tag="ACG8WP_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026546.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55837..55910
                     /locus_tag="ACG8WP_000204"
     tRNA            55837..55910
                     /locus_tag="ACG8WP_000204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55871..55873,aa:Arg,seq:cct)
     gene            complement(55979..57118)
                     /locus_tag="ACG8WP_000205"
     CDS             complement(55979..57118)
                     /locus_tag="ACG8WP_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9026547.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57417..58052)
                     /locus_tag="ACG8WP_000206"
     CDS             complement(57417..58052)
                     /locus_tag="ACG8WP_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026548.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58165..58800)
                     /locus_tag="ACG8WP_000207"
     CDS             complement(58165..58800)
                     /locus_tag="ACG8WP_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP9026549.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58834..59295)
                     /locus_tag="ACG8WP_000208"
     CDS             complement(58834..59295)
                     /locus_tag="ACG8WP_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFP9026550.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59425..59856)
                     /locus_tag="ACG8WP_000209"
     CDS             complement(59425..59856)
                     /locus_tag="ACG8WP_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9026551.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59874..60194)
                     /locus_tag="ACG8WP_000210"
     CDS             complement(59874..60194)
                     /locus_tag="ACG8WP_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9026552.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60493..61269
                     /locus_tag="ACG8WP_000211"
     CDS             60493..61269
                     /locus_tag="ACG8WP_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9026553.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61284..61487
                     /locus_tag="ACG8WP_000212"
     CDS             61284..61487
                     /locus_tag="ACG8WP_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026554.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61503..61841
                     /locus_tag="ACG8WP_000213"
     CDS             61503..61841
                     /locus_tag="ACG8WP_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9026555.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADNTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        20397 a        10406 c        12980 g        18070 t
ORIGIN      
        1 aagggaaaca tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa
       61 ggattgaata aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa
      121 gttctataac gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa
      181 tttttggttt gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag
      241 gaaatctgat atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta
      301 catattatga aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca
      361 accacgaaga tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa
      421 aaaagagaaa tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt
      481 caaaaacggc atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag
      541 gaatcacagc atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat
      601 ttttgtaaga atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat
      661 gaaacacgag aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg
      721 aagaggagga gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta
      781 ttggatttta tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa
      841 gaagctcatg tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt
      901 ttacttggac agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca
      961 tcaagtgtca tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc
     1021 gatgtcatga tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg
     1081 actttgcttg aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt
     1141 atttttatgg atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg
     1201 ttaagaaaag gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc
     1261 ccagatgaag agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt
     1321 cgtgttgtga tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg
     1381 atcttaatga caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt
     1441 agataatcga ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta
     1501 agtcaattaa aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct
     1561 cctgttttca attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg
     1621 atttagtagt tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga
     1681 cagaagcaat ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc
     1741 agctggatag agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac
     1801 gctaaaaaac cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata
     1861 ttgtttgttt tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg
     1921 agcaaaaaaa taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt
     1981 ttagtgactc tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact
     2041 gattctgaca cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct
     2101 gttcaaacat tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt
     2161 tatggagctt atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt
     2221 ttttttgttg cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt
     2281 gggctagctc tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt
     2341 atctaacagg aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg
     2401 aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt
     2461 cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg
     2521 ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg
     2581 gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt
     2641 tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt
     2701 ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat
     2761 ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg
     2821 taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt
     2881 tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga
     2941 taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt
     3001 ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg
     3061 caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa
     3121 cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag
     3181 tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg
     3241 cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag
     3301 gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc
     3361 gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag
     3421 ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag
     3481 cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg
     3541 agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta
     3601 cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt
     3661 ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg
     3721 ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt
     3781 tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg
     3841 tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg
     3901 aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag
     3961 aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag
     4021 ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg
     4081 aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata
     4141 ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg
     4201 attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca
     4261 ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg
     4321 atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt
     4381 cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa
     4441 tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt
     4501 attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc
     4561 ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct
     4621 aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt
     4681 aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa
     4741 agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat
     4801 tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa
     4861 agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg
     4921 gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca
     4981 gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga
     5041 aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg
     5101 aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt
     5161 ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag
     5221 gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca
     5281 aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag
     5341 tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa
     5401 aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc
     5461 tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag
     5521 cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga
     5581 agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat
     5641 tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc
     5701 agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc
     5761 tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga
     5821 ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc
     5881 aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg
     5941 agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa
     6001 caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     6061 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     6121 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     6181 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     6241 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     6301 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     6361 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     6421 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     6481 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     6541 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     6601 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     6661 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     6721 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     6781 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     6841 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     6901 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     6961 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     7021 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     7081 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     7141 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     7201 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     7261 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     7321 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     7381 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     7441 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     7501 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     7561 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     7621 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     7681 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     7741 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     7801 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     7861 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     7921 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     7981 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     8041 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     8101 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     8161 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     8221 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     8281 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     8341 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     8401 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     8461 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     8521 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     8581 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     8641 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     8701 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     8761 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     8821 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     8881 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     8941 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     9001 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     9061 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     9121 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     9181 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     9241 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     9301 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     9361 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     9421 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     9481 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     9541 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     9601 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     9661 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     9721 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     9781 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     9841 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     9901 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     9961 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
    10021 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
    10081 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
    10141 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
    10201 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
    10261 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
    10321 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
    10381 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
    10441 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
    10501 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
    10561 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
    10621 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
    10681 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
    10741 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
    10801 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
    10861 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
    10921 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
    10981 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
    11041 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
    11101 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
    11161 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
    11221 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
    11281 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
    11341 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
    11401 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
    11461 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
    11521 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
    11581 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
    11641 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
    11701 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
    11761 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
    11821 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
    11881 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
    11941 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
    12001 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
    12061 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
    12121 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
    12181 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
    12241 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
    12301 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
    12361 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
    12421 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
    12481 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
    12541 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
    12601 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
    12661 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
    12721 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
    12781 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
    12841 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
    12901 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
    12961 cgtttacggg tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt
    13021 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
    13081 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
    13141 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
    13201 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
    13261 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
    13321 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
    13381 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
    13441 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
    13501 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
    13561 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
    13621 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
    13681 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
    13741 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
    13801 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
    13861 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
    13921 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
    13981 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
    14041 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
    14101 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
    14161 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
    14221 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
    14281 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
    14341 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
    14401 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
    14461 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
    14521 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
    14581 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
    14641 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
    14701 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
    14761 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
    14821 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    14881 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    14941 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    15001 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    15061 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    15121 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    15181 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    15241 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    15301 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    15361 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    15421 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    15481 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    15541 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    15601 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    15661 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    15721 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    15781 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    15841 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    15901 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    15961 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    16021 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    16081 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    16141 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    16201 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    16261 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    16321 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    16381 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    16441 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    16501 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    16561 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    16621 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    16681 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    16741 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    16801 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    16861 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    16921 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    16981 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    17041 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    17101 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    17161 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    17221 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    17281 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    17341 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    17401 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    17461 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    17521 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    17581 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    17641 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    17701 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    17761 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    17821 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    17881 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    17941 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    18001 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    18061 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    18121 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    18181 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    18241 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    18301 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    18361 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    18421 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    18481 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    18541 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    18601 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    18661 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    18721 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    18781 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    18841 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    18901 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    18961 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    19021 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    19081 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    19141 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    19201 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    19261 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    19321 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    19381 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    19441 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    19501 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    19561 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    19621 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    19681 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    19741 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    19801 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    19861 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    19921 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    19981 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    20041 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    20101 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    20161 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    20221 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    20281 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    20341 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    20401 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    20461 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    20521 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    20581 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    20641 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    20701 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    20761 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    20821 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    20881 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    20941 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    21001 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    21061 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    21121 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    21181 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    21241 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    21301 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    21361 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    21421 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    21481 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    21541 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    21601 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    21661 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    21721 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    21781 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    21841 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    21901 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    21961 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    22021 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    22081 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    22141 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    22201 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    22261 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    22321 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    22381 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    22441 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    22501 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    22561 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    22621 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    22681 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    22741 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    22801 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    22861 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    22921 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    22981 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    23041 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    23101 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    23161 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    23221 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    23281 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    23341 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    23401 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    23461 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    23521 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    23581 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    23641 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    23701 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    23761 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    23821 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    23881 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    23941 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    24001 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    24061 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    24121 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    24181 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    24241 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    24301 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    24361 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    24421 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    24481 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    24541 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    24601 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    24661 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    24721 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    24781 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    24841 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    24901 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    24961 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
    25021 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    25081 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    25141 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    25201 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    25261 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    25321 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    25381 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    25441 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    25501 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    25561 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    25621 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    25681 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    25741 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    25801 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    25861 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    25921 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    25981 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    26041 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    26101 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    26161 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    26221 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
    26281 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    26341 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    26401 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    26461 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    26521 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    26581 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    26641 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    26701 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    26761 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    26821 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    26881 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    26941 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    27001 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    27061 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    27121 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    27181 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    27241 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    27301 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    27361 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    27421 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    27481 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    27541 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    27601 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    27661 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    27721 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    27781 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    27841 gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    27901 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    27961 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    28021 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    28081 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    28141 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    28201 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    28261 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    28321 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    28381 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    28441 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    28501 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    28561 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    28621 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    28681 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    28741 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    28801 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    28861 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    28921 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    28981 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    29041 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    29101 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    29161 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    29221 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    29281 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    29341 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    29401 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    29461 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    29521 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    29581 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    29641 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    29701 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    29761 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    29821 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    29881 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    29941 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    30001 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    30061 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    30121 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    30181 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    30241 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    30301 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    30361 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    30421 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    30481 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    30541 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    30601 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    30661 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    30721 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    30781 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
    30841 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
    30901 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
    30961 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
    31021 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
    31081 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
    31141 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
    31201 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
    31261 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
    31321 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
    31381 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
    31441 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
    31501 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
    31561 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
    31621 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
    31681 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
    31741 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
    31801 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
    31861 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
    31921 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
    31981 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
    32041 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
    32101 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
    32161 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
    32221 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
    32281 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
    32341 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
    32401 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
    32461 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
    32521 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    32581 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    32641 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    32701 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    32761 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    32821 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    32881 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    32941 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    33001 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    33061 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    33121 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    33181 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    33241 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    33301 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    33361 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    33421 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    33481 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    33541 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    33601 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    33661 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    33721 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    33781 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    33841 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    33901 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    33961 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    34021 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    34081 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    34141 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    34201 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    34261 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    34321 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    34381 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    34441 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    34501 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    34561 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    34621 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    34681 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    34741 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    34801 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    34861 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    34921 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    34981 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    35041 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    35101 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    35161 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    35221 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    35281 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    35341 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    35401 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    35461 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    35521 atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca
    35581 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    35641 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    35701 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    35761 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    35821 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    35881 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    35941 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    36001 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    36061 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    36121 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    36181 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    36241 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    36301 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    36361 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    36421 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    36481 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    36541 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    36601 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    36661 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    36721 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    36781 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    36841 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    36901 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    36961 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    37021 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    37081 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    37141 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    37201 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    37261 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    37321 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    37381 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    37441 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    37501 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    37561 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    37621 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    37681 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    37741 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    37801 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    37861 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    37921 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    37981 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    38041 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    38101 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    38161 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    38221 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    38281 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    38341 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    38401 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    38461 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    38521 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    38581 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    38641 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    38701 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    38761 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    38821 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    38881 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    38941 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    39001 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    39061 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    39121 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    39181 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    39241 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    39301 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    39361 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    39421 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    39481 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    39541 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    39601 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    39661 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    39721 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    39781 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    39841 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    39901 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    39961 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    40021 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    40081 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    40141 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    40201 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    40261 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    40321 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    40381 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    40441 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    40501 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    40561 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    40621 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    40681 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    40741 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    40801 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    40861 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    40921 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    40981 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    41041 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    41101 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    41161 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    41221 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    41281 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    41341 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    41401 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    41461 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    41521 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    41581 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    41641 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    41701 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    41761 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    41821 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    41881 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    41941 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    42001 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    42061 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    42121 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    42181 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    42241 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    42301 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    42361 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    42421 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    42481 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    42541 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    42601 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    42661 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    42721 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    42781 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    42841 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    42901 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    42961 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    43021 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    43081 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    43141 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    43201 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    43261 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    43321 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    43381 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    43441 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    43501 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    43561 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    43621 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    43681 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    43741 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    43801 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    43861 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    43921 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    43981 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    44041 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    44101 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    44161 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    44221 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    44281 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    44341 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    44401 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    44461 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    44521 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    44581 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    44641 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    44701 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    44761 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    44821 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    44881 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    44941 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    45001 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    45061 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    45121 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    45181 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    45241 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    45301 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    45361 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    45421 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    45481 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    45541 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    45601 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    45661 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    45721 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    45781 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    45841 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    45901 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    45961 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    46021 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    46081 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    46141 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    46201 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    46261 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    46321 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    46381 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    46441 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    46501 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    46561 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    46621 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    46681 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    46741 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    46801 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    46861 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    46921 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    46981 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    47041 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    47101 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    47161 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    47221 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    47281 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    47341 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    47401 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    47461 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    47521 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    47581 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    47641 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    47701 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    47761 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    47821 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    47881 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    47941 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    48001 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    48061 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    48121 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    48181 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    48241 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    48301 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    48361 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    48421 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    48481 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    48541 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    48601 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    48661 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    48721 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    48781 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    48841 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    48901 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    48961 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    49021 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    49081 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    49141 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    49201 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    49261 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    49321 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    49381 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    49441 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    49501 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    49561 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    49621 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    49681 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    49741 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    49801 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    49861 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    49921 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    49981 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    50041 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    50101 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    50161 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    50221 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    50281 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    50341 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    50401 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    50461 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    50521 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    50581 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    50641 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    50701 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    50761 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    50821 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    50881 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    50941 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    51001 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    51061 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    51121 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    51181 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    51241 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    51301 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    51361 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    51421 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    51481 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    51541 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    51601 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    51661 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    51721 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    51781 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    51841 ttagttgctt tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc
    51901 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    51961 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    52021 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    52081 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    52141 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    52201 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    52261 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    52321 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    52381 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    52441 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    52501 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    52561 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    52621 ttctttggta ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    52681 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    52741 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    52801 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    52861 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    52921 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    52981 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    53041 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    53101 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    53161 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    53221 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    53281 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    53341 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    53401 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    53461 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    53521 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    53581 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    53641 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    53701 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    53761 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    53821 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    53881 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    53941 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    54001 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    54061 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    54121 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    54181 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    54241 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    54301 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    54361 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    54421 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    54481 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    54541 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    54601 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    54661 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    54721 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    54781 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    54841 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    54901 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    54961 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    55021 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    55081 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    55141 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    55201 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    55261 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    55321 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    55381 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    55441 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    55501 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    55561 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    55621 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    55681 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    55741 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    55801 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    55861 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac
    55921 ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact
    55981 agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt
    56041 caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta
    56101 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat
    56161 gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca
    56221 aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat
    56281 tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt
    56341 ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg
    56401 tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt
    56461 cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac
    56521 cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt
    56581 tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat
    56641 cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg
    56701 catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt
    56761 ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata
    56821 ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag
    56881 gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata
    56941 gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat
    57001 cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat
    57061 cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa
    57121 ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt
    57181 ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt
    57241 aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt
    57301 gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt
    57361 ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa
    57421 gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt
    57481 ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat
    57541 aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca
    57601 gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt
    57661 aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga
    57721 agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt
    57781 tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg
    57841 aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag
    57901 aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca
    57961 acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct
    58021 tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt catttccttt
    58081 acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct
    58141 aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca
    58201 ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac
    58261 tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga
    58321 aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat
    58381 aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc
    58441 actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact
    58501 atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag
    58561 gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt
    58621 gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat
    58681 cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat
    58741 taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat
    58801 taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt
    58861 aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat actttttaat
    58921 ttcatctgca cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt
    58981 ctctatatat ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt
    59041 gttttcatct ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt
    59101 aaattgtata taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt
    59161 aaaggggata gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct
    59221 aataattgaa atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa
    59281 aatctctttt tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc
    59341 atatatgagg aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg
    59401 tttttttaat aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt
    59461 aaaatagcat tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata
    59521 ctccataaat ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat
    59581 atttaataaa attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt
    59641 tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc
    59701 atgagataaa tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat
    59761 tctgggtttt ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt
    59821 gtattcgctc aacatttctt tcaatttcaa atacatacaa accatcactc caactattca
    59881 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    59941 tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct
    60001 ctttcttctc tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt
    60061 aattcatctg aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca
    60121 aaattaattt ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt
    60181 tcataggtat tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa
    60241 tacgcagaaa taatttgact aattctgagt aatgagctat actatatctt gtaaacagat
    60301 ttaacaacta aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa
    60361 gctttaaaat caatgttttt atgtcttatt taattatgct cttagtatag agtattactc
    60421 agatactgtc aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    60481 ggaatgagaa atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca
    60541 attttagtaa atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat
    60601 tcaaacacta aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga
    60661 atcacgacct caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt
    60721 ttaatcttaa aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa
    60781 gtgttgccag caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct
    60841 ttgcttaatc cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga
    60901 agattaatcg cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc
    60961 atattatctt ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct
    61021 ccacaacaga tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa
    61081 tggttattgt gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc
    61141 ccgaaagccg atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt
    61201 ctatttatct acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa
    61261 attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag
    61321 aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa
    61381 gatttggaat tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag
    61441 aagcaaacag aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc
    61501 taatgcaata tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag
    61561 tggattatga ggagttaaaa aaagctgata atactggtag atggatgacg ttgccagaag
    61621 tgctagaacg gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa
    61681 gatatagaaa tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg
    61741 aaggaagaga cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt
    61801 tttcggaaat cttaaggagg caagcggatg ggaaaattta atagagcgct agt
//
LOCUS       DAZKAY010000004        60329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000004 DAZKAY010000000
VERSION     DAZKAY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.004"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8WP_000214"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8WP_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9026556.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="ACG8WP_000215"
     CDS             complement(1692..2711)
                     /locus_tag="ACG8WP_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026557.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="ACG8WP_000216"
     CDS             complement(2733..3740)
                     /locus_tag="ACG8WP_000216"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9026558.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8WP_000217"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8WP_000217"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9026559.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="ACG8WP_000218"
     CDS             complement(4391..6685)
                     /locus_tag="ACG8WP_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9026560.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="ACG8WP_000219"
     CDS             6945..9110
                     /locus_tag="ACG8WP_000219"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9026561.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="ACG8WP_000220"
     CDS             9414..10247
                     /locus_tag="ACG8WP_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9026562.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="ACG8WP_000221"
     CDS             complement(10349..12049)
                     /locus_tag="ACG8WP_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026563.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="ACG8WP_000222"
     CDS             12556..13716
                     /locus_tag="ACG8WP_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9026564.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="ACG8WP_000223"
     CDS             13732..14175
                     /locus_tag="ACG8WP_000223"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9026565.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="ACG8WP_000224"
     CDS             complement(14658..15188)
                     /locus_tag="ACG8WP_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9026566.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="ACG8WP_000225"
     CDS             15293..15844
                     /locus_tag="ACG8WP_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9026567.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="ACG8WP_000226"
     CDS             complement(15997..16470)
                     /locus_tag="ACG8WP_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9026568.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="ACG8WP_000227"
     CDS             16608..18080
                     /locus_tag="ACG8WP_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9026569.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="ACG8WP_000228"
     CDS             18077..18787
                     /locus_tag="ACG8WP_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9026570.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="ACG8WP_000229"
     CDS             19139..19423
                     /locus_tag="ACG8WP_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9026571.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="ACG8WP_000230"
     CDS             complement(19740..20123)
                     /locus_tag="ACG8WP_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9026572.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="ACG8WP_000231"
     CDS             complement(20125..20709)
                     /locus_tag="ACG8WP_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9026573.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="ACG8WP_000232"
     CDS             complement(20756..22231)
                     /locus_tag="ACG8WP_000232"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9026574.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="ACG8WP_000233"
     CDS             22412..22723
                     /locus_tag="ACG8WP_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026575.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="ACG8WP_000234"
     CDS             complement(22838..25174)
                     /locus_tag="ACG8WP_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9026576.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="ACG8WP_000235"
     CDS             25316..25984
                     /locus_tag="ACG8WP_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9026577.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="ACG8WP_000236"
     CDS             25987..27030
                     /locus_tag="ACG8WP_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9026578.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="ACG8WP_000237"
     CDS             complement(27267..28148)
                     /locus_tag="ACG8WP_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9026579.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="ACG8WP_000238"
     CDS             28307..29035
                     /locus_tag="ACG8WP_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9026580.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="ACG8WP_000239"
     CDS             29191..29508
                     /locus_tag="ACG8WP_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9026581.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="ACG8WP_000240"
     CDS             29501..30109
                     /locus_tag="ACG8WP_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9026582.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="ACG8WP_000241"
     CDS             30164..30955
                     /locus_tag="ACG8WP_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9026583.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="ACG8WP_000242"
     CDS             31093..31581
                     /locus_tag="ACG8WP_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9026584.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="ACG8WP_000243"
     CDS             31632..32504
                     /locus_tag="ACG8WP_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9026585.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="ACG8WP_000244"
     CDS             32680..33042
                     /locus_tag="ACG8WP_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9026586.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="ACG8WP_000245"
     CDS             33051..33803
                     /locus_tag="ACG8WP_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026587.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="ACG8WP_000246"
     CDS             33817..35619
                     /locus_tag="ACG8WP_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026588.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="ACG8WP_000247"
     CDS             35896..36936
                     /locus_tag="ACG8WP_000247"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9026589.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(36988..39132)
                     /locus_tag="ACG8WP_000248"
     CDS             complement(36988..39132)
                     /locus_tag="ACG8WP_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9026590.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(39489..40862)
                     /locus_tag="ACG8WP_000249"
     CDS             complement(39489..40862)
                     /locus_tag="ACG8WP_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9026591.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(40862..41257)
                     /gene="rbsD"
                     /locus_tag="ACG8WP_000250"
     CDS             complement(40862..41257)
                     /gene="rbsD"
                     /locus_tag="ACG8WP_000250"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9026592.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(41285..42202)
                     /gene="rbsK"
                     /locus_tag="ACG8WP_000251"
     CDS             complement(41285..42202)
                     /gene="rbsK"
                     /locus_tag="ACG8WP_000251"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9026593.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(42310..43323)
                     /locus_tag="ACG8WP_000252"
     CDS             complement(42310..43323)
                     /locus_tag="ACG8WP_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9026594.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(43404..44003)
                     /locus_tag="ACG8WP_000253"
     CDS             complement(43404..44003)
                     /locus_tag="ACG8WP_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9026595.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            44189..44890
                     /locus_tag="ACG8WP_000254"
     CDS             44189..44890
                     /locus_tag="ACG8WP_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026596.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            44900..47611
                     /locus_tag="ACG8WP_000255"
     CDS             44900..47611
                     /locus_tag="ACG8WP_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9026597.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            48062..49690
                     /gene="treC"
                     /locus_tag="ACG8WP_000256"
     CDS             48062..49690
                     /gene="treC"
                     /locus_tag="ACG8WP_000256"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9026598.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(49750..50931)
                     /locus_tag="ACG8WP_000257"
     CDS             complement(49750..50931)
                     /locus_tag="ACG8WP_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9026599.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            51183..52706
                     /locus_tag="ACG8WP_000258"
     CDS             51183..52706
                     /locus_tag="ACG8WP_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9026600.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(52775..53503)
                     /locus_tag="ACG8WP_000259"
     CDS             complement(52775..53503)
                     /locus_tag="ACG8WP_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9026601.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(53516..54796)
                     /locus_tag="ACG8WP_000260"
     CDS             complement(53516..54796)
                     /locus_tag="ACG8WP_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9026602.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(54937..55539)
                     /locus_tag="ACG8WP_000261"
     CDS             complement(54937..55539)
                     /locus_tag="ACG8WP_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9026603.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            55784..55990
                     /locus_tag="ACG8WP_000262"
     CDS             55784..55990
                     /locus_tag="ACG8WP_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026604.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            56302..57021
                     /locus_tag="ACG8WP_000263"
     CDS             56302..57021
                     /locus_tag="ACG8WP_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9026605.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(57102..57785)
                     /locus_tag="ACG8WP_000264"
     CDS             complement(57102..57785)
                     /locus_tag="ACG8WP_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9026606.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            58005..60191
                     /locus_tag="ACG8WP_000265"
     CDS             58005..60191
                     /locus_tag="ACG8WP_000265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9026607.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        19233 a        10759 c        12219 g        18118 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaaag ggagtgtgac
    36961 agtgtcacat ccccttttta atttattcta tttctttaaa ggcagatcaa gccagaatga
    37021 tttgtctaaa cttaacaagg ttagtacagg aacagttgca gctttaactg tacgagtagt
    37081 tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa tcccagattg aagcgacacg
    37141 gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa aattctaatc ctgtgattgg
    37201 cacgccatct gttgtccatt tgattggcac atcgaatcgc ttagacatat tgaggatttc
    37261 agcgtttgct tcttcagttg ttgctgcttc ttttttcggt tcatcatatt ttgtcaggtc
    37321 gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg cgatctgtag cataacgtcc
    37381 ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc gacttccaag ttggtttgta
    37441 gaaatcagga ttgccagtca gtccattttt gatcaagtcg gcatagtctt ccaaactttc
    37501 cttgtaagaa ggatatttac ggaattgtgc gcggatttgg tagctttgtc cggaactatc
    37561 ttgttcaagt gtattgaagg tcacgctgct tcctttatat gatcctttga taccaaaaag
    37621 gttatgattt ggttcgcttg ataataagct gtttccagat ccactttcaa ggatagcttg
    37681 agccatcatg actgatgcgt agattccttc tttttgcccg atttcctgtg catcatctgc
    37741 aatctttttc aagaactgat cagttgtttg gttttgactg aaatggaacg agccatctaa
    37801 tccatctaaa atagatgagc cgcctgtcag cgaacctgaa gcagcaggta aacctgctaa
    37861 gtttactcgg acagcaaaat caggcgtgaa atctgaaata gaagttactc gaactttatc
    37921 tcctggttga ggcgcatgga taaattgtcc gttcccaata tagataccta catggtgagt
    37981 aacaccggaa gaaggtcccc agaagacgag gtcgccaggt tgggcctgac ttactggaat
    38041 ctgcgtacct gcatattgct gttcgcctgt ccagccaccg atataatgac cggttgcttt
    38101 catatatacg taataagtca aaccagaaca gtcaaaagta tttggacctt ttgctcccca
    38161 tacatatggt tttccaagat gcttgaatgc ttctcgaacg attgcttgtt ggacattcgt
    38221 tgtatttgta ggcaatacag catcaattgg tgattttaca acaaaatcag aagaagcaga
    38281 agatggattc tctataccgc tttgatttgc tgtatttgga cgagatgaag cattgttcgt
    38341 tccgccattc gatgaaccat tgtttggatg gtttggttct ctctgagtat ttccattgtt
    38401 attcgatcca gatgggtttg tttgattcga agaattcccg ctgttcgaat tattgttatt
    38461 attgtttgaa ttatttgaac cattattatt gttgttatta cccacatctg gtttgtttgg
    38521 cgtttccgga tctggtgtac tagggacttc ttcactagga tcagttggtt tctctgttga
    38581 agtatccggt ccagctttaa attctgcagt aatagataaa ttgttcgtac ctactgtaaa
    38641 ggtctttgca tcataattta cttctttggc accattaaca gcgactgaag caatttgttc
    38701 accttcagct ggcgtaatag tgtaagttac cttcgtttcc gctaataaac cttctaccgt
    38761 tccactttcc gtaatatcca aaggttgctg ttcaggggaa tccgctgtcg aagcagtcag
    38821 ttggatctta ccaatcaaag aaggagcaat tgtaagggaa tatgtctcgg ctggcttttc
    38881 attttctgat ggatcagccg gatcaatttc agttggctcg gtcgatcctg ttgttgagtt
    38941 agttgttcca gttgggtctt ctgtaaaaga atccgttgtt tcactagtag ctgagctgga
    39001 tgtagtcgta tctgttgtga ctgtttgatc agctatttga ttatctacgg caaatacggt
    39061 attggtcgac aaaacagacg gagcaatcag ccaagtagca gctagcaaac gtatcgtttt
    39121 tttatttttc ataataaact agtccttttc ctttcattga ttttctgtct tattcagcat
    39181 tcattagaca ttttttggaa cagataatcg tgtcgaaaag ctttttcttt tcataaatct
    39241 gtctgtgtat tctaaataaa acaatttgta aataagcaac gagcattcat tcgtttttct
    39301 ttgacgttca ttttattgca tagaccacct ttgtttgggg ataaacaagt ggggataaaa
    39361 atggttatgc atatgtaaat tataccaaag tttggaaagt atcacaaaaa tttataaaaa
    39421 taatgatcct aaaaagaaaa agactgaaat atgtgataaa catctcatac ttcagtcttt
    39481 ttcattaact attcataaga cattaggtct tcatcaaacc cgctaacgag agtagtgatc
    39541 attgctacga ctagactaac agccagtcct gctaagtacc agaaaaaagt accggaaatg
    39601 aatgcaggca atgtggtcag actaccaaat acaaaagcga tcgctttcac ttgggcaaat
    39661 cccattacag ctccaccagc agctgcacca atgcaagcag caacaaatgg ttttttcaat
    39721 gggatcgcaa ttccgtaaac agttggttcg gtcactcccg caagtaaaat cgaagcaaaa
    39781 gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc gcagaaatac acctaaagct
    39841 gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa cgattcggtc gaacccataa
    39901 gcagagatat tactgatcat gacagggttt acagcccatt ggataccaaa tattaccaaa
    39961 atattccaac caccagcgat gattgcgcca ctgatcggtc cgttaatatc catcatccaa
    40021 gtaattgcgg cagctaaacc ttcgccaagg taaacacccg caggcccgaa taccattaac
    40081 gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa atactaattg catattttcg
    40141 cgaatatatt ttttgaggaa tttttcaaca aaagaataaa agaaaatacc taaaatcgcc
    40201 ggcatgacag tagacgtata ctgcatcagt actacaggaa tccccagaaa atcagtagct
    40261 gctttgccac tgtctgaaag taaagaggtc agattcggtt caatcagtgc tgcagcaatg
    40321 acagccgcaa tatatccatt cactttaaac gttttggcag cagaaaaagc aagaaagatt
    40381 ggcagaaaat aaaagacggc attcgacgca gcagacaaaa tatgataagt tccgcttgtt
    40441 tcactaagcc agccaatcgt agtcgctaaa acaaccagac cttttaatat accagaaccg
    40501 gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca aaccaaatag tttttcgatc
    40561 ggatttagct tttctttcgg agctgaatga tcgatttgac gcgtcaattc cttgtaagca
    40621 ggagccaatc cctgatccat gattatttct gcatatctat cgagtaaaat gaaatactta
    40681 atggaaggga tttgctgttt taatttttct gcattggcta ctggctgttt tttcaatttg
    40741 atacgaagcc gagtcgcaca tggaaggaca gaatcaatat tgtcttttcc tcccaactgt
    40801 tcaatcagca tatgaatctg ctcggacagt accatctgtt caggattcat tgtcgtttcc
    40861 attagaagac cactccactc actaatataa tattggcgta aggtgtcatt tcgcctgtac
    40921 gaataaatgc atgacagtta ttcaactctt ttttcatttc ttcatgtggg ataaagctaa
    40981 tcggtgtatc tggtaacaat aatttgattt tttccaatat ttccgggttc attgtcttta
    41041 tttcttccgc caattcaatc gattgtacct caagttcttc taaaacgttt tctaaaacag
    41101 ataaaaaact tggaagaccg tttgagacag ccaaatctat tttttcagta ccaaagggga
    41161 caggcatgcc tgcatccccg atacttaatt tatcaaagtg ccccatttgt gcgattactc
    41221 gtgaaatatc tgaattgatt acttttcctt ttttcatgtt tgtttcttcc tctcattgtt
    41281 aattttagac tgctgattgt tctaaagctt cgcggtaagg gatagaaggt tgcgcaccat
    41341 aagactgcac agcaacagaa gaggctagat ttccataaag gatcgcttct ttcaagttac
    41401 taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa tgtatcacct gcggctgttg
    41461 tgtcaacggc atccacttta aaagcaggga cgataccgtg ttctttttct gtatgataga
    41521 aagcgccggc actgcctagt gtaatgatta ctgttccaat acctaattca tggagttttt
    41581 cggctgctgc gaccaaactg ttatgatctg tcactctgat tcctgtaatg atttctgttt
    41641 ctgtttcatt tggtataatg atatctgtct tttcaagtag ttcagtagga atcgtttcac
    41701 gagctggtgc aggattcagg atcgttgttt ttcctgcgtc ttttgcaata gaaaaagcta
    41761 ggatcgtcgc atctaagcta ctttcaaact gagcaataat aaagtcactg tttgtaatca
    41821 gttgcttgtt ttttaggact tcttgttcat ggaaagcatt atttgcccct gcatggatca
    41881 agatactatt ttctcctgct gcgtctacca taatcattgc ttgtccggtt gtggcgcctt
    41941 ggatttcagc tatcccagat gtatcgatgt tttcttttgt cagtagatct aataactgtt
    42001 gtccttcgct gtcagcccca acgcctccaa taaaagatgt ccgtgcgcct gaacgtttgg
    42061 ctgccactgc ttggttggca cctttacctc cgccagcgtg aaaaatctca tgcgcatgca
    42121 tcgtttcgcc tggcttgggc atgttggtta aacgaagggt agtgtccata ttaatacttc
    42181 caattactgt aaccgtgttc attttacaaa cctccaacta tctcatttat tttgatggga
    42241 aaattatttt ttaaaacgtt ttagtaaaac gttttaatag ttcgataaaa atatagcaca
    42301 ccttgaaagc tatttcaagg gtgcagttga aaatcttttt tctaatcgta caggtaatct
    42361 tttttcttcc cattcttttt ctgggaattg gatccgatct aacaataact tggcagatgt
    42421 ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt ttcggcttga tatactcgca
    42481 catatctata ttatcatagc caataatgct gtagtcttca gggatgcttt ttccagcttc
    42541 ctctaatccg cgatataatc caaaagctaa ttcatcattt aaagcaaaca caccagttgc
    42601 agcagaatca agaagttctg ctgtgatctg gtagccacct tgttttgaga actgtgtagg
    42661 taagaggatc agttggtcgt gagggatttg gtaaacatca agagcagatt taaacccctc
    42721 aatccgagcg tggacatttt ccggtggatt ttcaggatag acaagggcga tcgtttgatg
    42781 tcctagacta agcaagtgca tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac
    42841 ggcatcacta aatccttcag attttttctg atccaaaaca atgaagggcc ggccttgttt
    42901 tttcaaattt tcgttgattg catctgtaga gacagcagaa gtcgcgataa tgaatccatc
    42961 cacacctcga cgtgtgagct cggctagata ttcgatttct ttttgatgat cagaatcagc
    43021 attacataat atcgtgacga aattctgctt atacaagata tcctcaattc ctcgcatcag
    43081 tgtactgaag aatggattcg tgatatcagg aacgagtaca ccaattgttt tcgtttcctt
    43141 ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct aattcttctt tggcagctaa
    43201 aactttctga acagttttcg gactgaattt ctccgtattt ccatttaata tcaaggagac
    43261 agttgtgatt gaaacgccgg aaagtttagc gacttctttg attgtgatct tttttttcac
    43321 catgttcata ctcctatcta aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa
    43381 atgaaaatca cgtgtgccag tacttatttg ctttgttgga tagcttcgat tatttgtttc
    43441 gttgagcgta ctcgtgcagt tttcggaaat atctttttta tgaacaattg atgggattct
    43501 ttgtcacgat cactgcaagc atcttcagca agcacgatat ggtacccgtg ttgaaaggca
    43561 tctaatgcag tagcatatac accgtttgtt gtcgctacac cgcttaaaat gatagtttcg
    43621 attccacgcc ggcgaagttg aagatcaaga gaagtaccaa aaaaagcact tggattgtat
    43681 tttgtgatag tcaacatatt atctgtatct ttcaaagaat ccttcaaaag taaatcagtg
    43741 aattctggag gtacagaagc agtcattcca cccatatcag tttgttgact caaataaggg
    43801 aaagtggaga tgtctacgtt cactaaaact ttcaaggcag cggtacgttt caatgttctt
    43861 actaacaaat cattgtttgt cagaatagat gctgcagtgt gtggagagag tgtaccgtta
    43921 tttacgatct ctttttgcat gtcgatcgcg acaaaagcag tttttgataa atcaaaaatc
    43981 atattttcct ttaaatcatt catgatgttc cttctttcaa ataacaatgt tttttatgta
    44041 agtaaatcat aaatcgttta tttgtcaact aaattaaagg attcctttct ttagtctaga
    44101 gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat tcgatgcata gatatagcga
    44161 gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc gaagtaaaaa atgaatacaa
    44221 acgatacaaa atgggagaaa cgaccattac agcaaatgac gatatttctt tttcaattga
    44281 aaaaggcgaa ttagtgatta tacttggtcc aagtggagca ggaaagtcaa cgattcttaa
    44341 tattttaggt gggatggata cacctgatga aggtcagatc attattgatg acacagatat
    44401 tgcgcaattt tcagataaac agcttactgc ttaccgacga acagatgtag ggttcgtttt
    44461 ccaattctac aatctcgttc ctaatctaac agcaaaagaa aatgtggaat tggcaacgga
    44521 agtttctcct gatgcgcttg atcctgtaga ggtattgagg caagtgggcc tagctcatcg
    44581 attgaataac tttccgtccc aattatcagg aggcgaacag cagagggttt caattgctcg
    44641 tgcactagct aaaaatccca aacttctttt atgtgatgaa ccgacaggcg cgctggattt
    44701 tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct agcagacaac acggcaatac
    44761 cgttttgatc attacacata attctgcttt agcgcccatc gctgatagag tcattcacat
    44821 taatgatgct aaagtaaggt ctgttgaatt aaatgatcat ccttcatcca ttgatgagat
    44881 tgtatggtag ggggatttta tgaaaacaag aacatatctt aaagcaggtt ttcgcgaaat
    44941 tcggcaatct aaaggacgat tcattgcaat catccttatt gtcatgctag gaacactttt
    45001 atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa acgatggatc actatgttgg
    45061 tacagcagga ctttctgatt tgcaaatcgt ttctactggt ggcttgacac aaaaagatat
    45121 tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca ggaaaacaga tctattattc
    45181 caatactggc aaaaacgaag tgattcaaat attttcttat aacaaaaata acaagcagaa
    45241 caagctacaa ttaactgatg gaaaactccc ggaaaaacca aaccagcttg tattggatga
    45301 aaaagcggaa gatgaagggt ataaaatcgg tgatatttat cagattgatt cagatgaatt
    45361 gaaagaaaaa gagtatacca tcacgggctt tgtccgttct ccattgttca tcaacaattt
    45421 ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat tattttgtct atttacctga
    45481 atccgttttt aaaacagata tttattctgt cttatatctt gattttacaa acgtaaagaa
    45541 tttgaatacg tacagcaaag cttacaaaga taaaatggag caaaatcaag aaaaggcaga
    45601 aaagtattta caagatcgac ccgaagaacg cttagaagag ctgaaaaaat cggccaacga
    45661 agaattagaa ccggcacagc aaaaaatagc ggatggcaaa gcacaaacaa cgcaagcaag
    45721 aacacggtta gaagatgcta aaaaacagtt agcgcaacag tcagcaacta tccagcagct
    45781 tcctgaagcg caacgaacaa tcgctgttca aacattatct caacaagaag ctcaacttgc
    45841 tgaacaagaa aggcaattag ttgagaaaga acgtgaactg gcctctgcag aggaagaatt
    45901 gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca tcatacctat acaatgaacg
    45961 ttcagaaaat gtcggttttc aagaatttgg tgatctgtct gatcgtatcg ctgcgattgc
    46021 caatgttttt cctgttttct ttttctttat tgccgcattg atcaccttta cgacaatgac
    46081 acgtatggta gaagaaaacc gcagggaaat cgggacatta aaggcgttag gttatacgaa
    46141 agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta gcctcaggga taggtattgt
    46201 tttaggaacg atattaggaa cgaatctttt gccaaggatc atttttgagc tgtctaacga
    46261 gcggtatgat attgggagtg cgcttatttt ttatgactgg ccgccgattt tacaagcagc
    46321 tgccgcattc ttcattgccg catttggcgc tgcaatgatc gttttattca aagatttaag
    46381 agaacggcct gctgcattgc ttcaaccgaa agcaccgaag ccagggaaac gcatttttct
    46441 tgaatacatc actccgctgt ggtcacgcct tagctttaat caaaaaatca gttaccgaaa
    46501 tctcttccga tataagtcaa gaatgcttat ggcgattatc ggaattgctg gatgtgccgg
    46561 gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt tctgtttcag ccaaacaatt
    46621 tggaccgatc attgactatc aagcaatcgt tacattggat tcatccacgg aagatagtcg
    46681 tcaaaaagca gcggatatgc tgcagaagca agaaaaagta aatgagatcc tagctattca
    46741 tactcaaacc gtagaattga gaaaaaaagg acatgcttcg caaagtctga cagtgatcgt
    46801 accaagtaaa acagctgatt tggatcgttt tatccattta actgatcttg aagagaaccc
    46861 acgctccttg cctaaaacag gaatcgctat tacgcaaaaa gtagcagaac tatttgactt
    46921 atctacagga gatcatattt cgctttatga tgaagatcaa aaagaatgga aagtcgaagt
    46981 aaaagaaatc gtgaaaaact atctaggtag ctttatttat atgtcccctg aatattatgc
    47041 ttcagttact gatcaatcga tgatggataa tgcatttttg ctgcgtacag ataagatgac
    47101 aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc acagacgctg tgacaaatac
    47161 gtctttcatg tccaaacaaa tcgaaacgca agaagaatca atggcgaact tagactcggt
    47221 tgtcttaatc tttgtcgtat tatcaggact gctggctttt atcgtcttgt ataatctaac
    47281 taatatcaat atttccgagc gtgtacgcga attatctaca ataaaagtcc ttggattttt
    47341 tgataaagaa gtcacgatgt acattgttcg agaaaatatc atctttactc ttttaggaat
    47401 catcggagga tttggtgtag gatatatatt gactgacttc atcttgcaac aagcgtctat
    47461 ggagaatgtc atctttccat tagtgatcac atggtgggct tatgtcttat cagcaggatt
    47521 aactatcgta tttacggtta ttgtgatgat tgttacccat ttcaaattaa agcatatcga
    47581 tatgattgat gcgttgaaat cgaatgaata aaaacagagg ttgtgaaatc agcggtttga
    47641 cctgagtatt agagaaacaa acggcaaaat agtttttcat attttgccgt tttttgagct
    47701 aataccgaag gacagctgat tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc
    47761 atcgagcaaa aagaatactc aaataaaatt agctcctcat gttttacatt tgagaagact
    47821 gattgccgta gataaaagct tttgaacacc atttatagaa gaaacaagaa tattttgcta
    47881 ttttaaacaa gaggatagac aaatacatgt ctgatctctt gttttttatt gggcaacaga
    47941 cctcttagaa tgtataaaga taaattttac aaacgctttc ggttcagtta taataataaa
    48001 tgataacttg tatatataag ttgtggtagg taataaatca gttgaatgga ggtaaatatc
    48061 catgacattt aaagacaaag tcatttacca aatctatccc aaatcttaca aagacagtaa
    48121 tggtgatgga attggagatt taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct
    48181 aggcatcgac atgatttggc taaaccctat ttatccaagc cctcaaaaag acaacggtta
    48241 tgatatctct gattacacag ccatcgatcc tatctttggt acgatggagg aattcgaaca
    48301 gctaatagcc gaagcaaaga aataccggat agaaatcatg ctcgatatgg tgctgaatca
    48361 cgtttcgaca gaacatgaat ggttcaaaaa agcactagca ggggaccagc attatcagga
    48421 cttcttcatt ttaagagaag agccaacgga ttgggtatca aaatttggtg gaagtgcttg
    48481 gtcgccattt ggagatacgg ggaaatatta tttgcactta tatgatcgga cacaagctga
    48541 cttgaactgg cggaaccaag cagtaagaga ggaactattc aaagttgttc gtttttggat
    48601 ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat gttatcggta aagacgaaca
    48661 attgaaaaat aataaagaaa atgatggcaa acctgaatat acggacaaac caatcaccca
    48721 tacgtatcta aaagaattga accaagcaac gtttgggacg gattccacta tcatcacagt
    48781 cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga
    48841 agaattatca atggtcttta atttccatca tttgaaagtc gattatgaaa atgggaataa
    48901 atggtctact cctccatttg attttgcaga attaaagcga ctatttcata catggggaga
    48961 aaagatgagt gagggtaacg gctggaatgc tctatttttt aataatcatg atcagccacg
    49021 tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa caaggagcag agatgctagc
    49081 aaccatgatc catttgaacc gtgggacgcc ctatatttat atgggagaag aaatcggcat
    49141 gatcgaccca gactattcat cgatttccga ctatattgac gtagaaagtt tgaatgctta
    49201 taagttactc cttgaacaag gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa
    49261 atctcgcgat aactctcgaa cacccatgca atggtcagac acagaacaag caggatttac
    49321 tacagggaaa ccttggttaa aagttggagg caagctagaa gagattaatg tagaaaaaga
    49381 actatcttct gaagattcta ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac
    49441 atatccaatc gttgcacaag gagattatca tgcttatgaa gcaaatcatc cggaagttta
    49501 tggctttctt cgccaatttg aaggacagca gctgcttgta ttaacaaatt tctatgataa
    49561 agatacaagg atcgccatcc cagaaggatt cacagaagcc aatattctta tcgataatta
    49621 ttccgatact gttcttcatc aaagtctgga gctaaaacca taccaggcaa ttgctttgct
    49681 gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag ctattctcgt aagatcgacg
    49741 ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa gagttttcat actctccttt
    49801 atcgccataa aatttattgt agcgttgttt gaatttacgg aaaataaaag taacgagtaa
    49861 tttgactaac gtatagatag gaataccaaa aataacgccc acaattccca ttagatcacc
    49921 catgacaagt aaaacgatta agatcgtaat cggatggatc tgtaaacgat cacccattac
    49981 tcgaggaatc accaagtcac cgtggagaag ttgtacggca aaccagacaa tgacaaattt
    50041 gacagccata aaagtagagt cttggaatgc aatgatcaaa cctggaaaga aggcgataaa
    50101 cggcccaata tatggaatga tacataacaa cccagcagaa atagctaaaa cactagcaaa
    50161 ttctaatccg atcagtaaga aacaaaccca gtagactgct ccaaggataa atgaagcaat
    50221 gatttgcccc tttagaaatg aacccaattg ttgattagca atctttgtca aatttttaaa
    50281 atctgtacga aatgctggtg ggacaatcgc taaaactgac tgataaaatt tttgaggatc
    50341 tctcaaaagg aaaaaagcga caattggacc agtaaataaa gtaataaaaa tagctgtaac
    50401 agttgaaaaa atgttgctga tccgttgtgc gccactttcc caataatttc caataaatcc
    50461 agctatatca gacgtcacgt cgtcaataga agcgaaaaat tgattaaatg aatcggcaaa
    50521 aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa
    50581 actactgatc aatgtctttg cctgttcttc aagaaaagga ataagccata tgcttccaag
    50641 ccccaataaa aataaaataa tcacataaac gataatgact gacaagttcc tcgaaaggtg
    50701 cttgctcaaa cgtttgatga ctggttccaa aagataatac aagatcagtg caaacaataa
    50761 tgggggcaag atagtagcga taatgattcc gacaggcttg aatataaacg agatctgatg
    50821 gaaaaaataa atgatcagcg caagtaagat aactattcct aaaacatagt aagaggtttt
    50881 tcctcctaaa aagcggatca aacgattcgt tgtatctgtt tcttttttca ctctttttcc
    50941 tcctttatct ataacttcat tatagacaaa agcgggtata aaaggaaaat gatagaggtt
    51001 gattgcaaaa atatcgtcaa tcattcatat aaactttctc atcaattaaa ataagaaaac
    51061 gcttgacagt tcttttaacc atatgataaa ttaaatcctg ttgctaaaac gtaactatta
    51121 cggtttgcga aaagcgaatc ggaacattca aatgaaagaa gatgagaaga ggagtgtcat
    51181 aaatgaaacg attgataggt atattggcca tgctggtttt agcaggtatg ttaactgctt
    51241 gcggggcaag tgggaaagcc gaggacagta aagaaaaatt gagcgtgatg acgacctttt
    51301 atcccatgta tgattttaca aaagcaatcg tcggggatga aggagaagta gagttattga
    51361 ttccagcagg gactgattcc cacgattatg aaccttctgc taaagacatg gcaaagatcc
    51421 aagatacaga tatctttgtt tataatgatg aaaacatgga aacatgggtc cccgcaatcc
    51481 aaaaaacatt acaagaaggc aatgtccata cgataaaagc gacagaaggt atgcttttac
    51541 ttccgggaag tgaagaagga catgatcatg accatgaaca cggggaagaa ggccatacac
    51601 atgagctaga cccacatgta tggctagctc caagtttagc aatcaagcag gtagcaaaca
    51661 tacgtgatca gctgattgaa gcttacccag aaaaacagga agtatggaca aaaaatgcag
    51721 cagcctatac cgagaaactt caagcactcc atcagcttta tcaagaaacc tttaagcaag
    51781 caaaacaaag atcttttgtc acgcaacatg ctgcattcaa ctatctagcg ttagaatatg
    51841 gattgaatca agtatcgatt gctggtcttt cttctagtga agaaccgtct gcggcgagaa
    51901 ttgctgaact gaaacacttt gttaaagaac atggaatcaa ctatatttat tttgaagaaa
    51961 acgccaaaga tagtattgcc agaacgttag caaacgaagc aggtgtatct ttagaagtat
    52021 tgaatccttt ggaaggattg acgaatgaac aaatagaaaa tggagaaaac tatctttcta
    52081 tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag
    52141 atactttgac ccccaaaaaa gaaaagagcg tctacaacgg ttattttgaa gacgaggatg
    52201 tacaagatcg ctctctttca gattgggcag gaacttggca aacagtagat acgtatgtag
    52261 aagatgggac ttttgatcct gtttttgaat ataaagaaaa attgaatcag gataagacag
    52321 cacaagaata taaagaatat tatacgaagg gctatcaaac agatattgct tcaatcaaga
    52381 tcagtgagaa agagatggca tttacgttta agaatggaac aacgaaacgt tcaaattatc
    52441 gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa ccgtggcgta aggtatctat
    52501 tcgaagcaga agataaggaa agtggcgctt ttagatatat tcaattcagt gatcacacga
    52561 ttgcaccggc tgactccgaa cattttcatt tatatgcagg aaatgaaagt caagaagtat
    52621 tgctgaaaga aatggagaat tggccaacat tttatccaga tacaatggat ggacgtgaaa
    52681 ttgcacagga aatgcttttg cactgatagg caaaaaagaa ccgaagggtt tggatcggct
    52741 ggtcatttag tcggtccaaa ctattcggtt ctttttatga aatcaatttc tttaatccac
    52801 ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac ttctctggtt tttttattaa
    52861 tcgaccgcac attttgtgta ttgataacat aggatttatg acaacggata aaattccgat
    52921 gcattttctc gatttctttg atcttctcat agaattgtaa ttgggcatga gtcaaatgga
    52981 cttccagttt atgtggcgct cctagcgaag tagaaaaata ttggatgtct ttgatcggta
    53041 aaaatttgat ctcatcatta aactctattt tgaagatatc tttttggatt tgggacattt
    53101 gaaaatcttc gaaaattttg ctcaggatat gctcgacatt ttcttttaaa tcataaagat
    53161 cttctttgac gatataatca gtcggttcga tgttgctttt taagattttc atcgacattt
    53221 ccgtatggga agtcacatag attatttttc ctaaaggatc taattcacgg atcaatttac
    53281 cgatcgctac accatccatt tcactgtctt ctagttcgat atctaaaaag taaataccaa
    53341 aagaagaagt tctgatcttg gctgcattga gaagttcttc cggatcagag gtagaaatat
    53401 ggacataact gtctaagttt tcaatcatga ttctattttt gatcaccgtt gataaaactt
    53461 ctaattgttt tggatcgtct tcacaaatat aaatgggaat catatcatta ctcctttatt
    53521 ttatttgtat ataaatcgtt tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa
    53581 gataaggatt ggtctttgtc aattcatcca agataaacaa gccaaggcct tcgttttttg
    53641 gatttgtttt tgttgagaac cctttttgtt tcaactggtt taacggtaat gtatttgttg
    53701 ttgtattgat cacacgaatc atcacggaat gattcttctt gagtatggtc aattcgattt
    53761 ttttcttctt tgcagcacta gctccttcaa tcgcattgtc caagataata ccaagcatac
    53821 gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga
    53881 cgatcaaccc tttttcctgt gccatcatga gttttaatga cagtaaacta cggatctcag
    53941 gaactttcat attatccaac atacttagat tggccggaaa aaggttcatc atctggcgtg
    54001 taggtaatat cgtttgatca taataagacc gtaatccttc taagtcgcct tctgaaatat
    54061 agctgtgaag agaaaaaagc agattttggt aatcatgttt gaattctctc aagtcttgat
    54121 agtttttttc tagatctttg atataatcct gttgcatcct gacttgttgc tctaataact
    54181 gatcagctaa tttcttatta gagattttcc aataataaac caagatgatc aggccaacac
    54241 acagatatag aatgaaaaaa ctcgtattca aagctaagac atcaggtgtt tcaccagaaa
    54301 aacgggtata aaaaataatg atatagtatg taaataatat caatgtcccg atcgtaccga
    54361 tcatacggcg gtccacagtg tgaaaccaag tctctaacga tttttttaaa caaaaagcta
    54421 ataaagcaga taaaaaagcg gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt
    54481 caataaccga agatttcaaa acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa
    54541 gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga
    54601 agaaaaaagt aagcatcagc attcctatag caattagagc agtaaaaatc cctaaaatcg
    54661 aatagaccca tccaatcaaa atacaagaaa tcagaatgac tcccgcaaac agatatttgt
    54721 atcggatgtg atggtcaatc aataagatac aaattgtgta aatcaaatat tgcaacgcta
    54781 tggaccataa aaacataaag tacagctccc attgattttt accaaattat agcacaattg
    54841 gcgtattatt tttttaattt ttcagggatt ttgaattcag aactcatacc aaagcagcag
    54901 ccagatacaa aaaagtcaga taagttagtt aattgtttaa tgattgtttt tttcatgaaa
    54961 gattcctcca aaattcatca agtagtttgt aataggaagc aacgtagcta tttctaaaac
    55021 aatcccaagt agcaaatagg tagaaaagta agaagctgag aaaaataacg taatgagaga
    55081 aaaaattaga aacatgctga tcgccttctt taataatggg ggacgtttct cgggttcaat
    55141 gggattgatt gctgtacctc ttgatccata gcgaagcagt aacaaaaagc agctgattaa
    55201 caaaaagacc atcattggga aggagatagt caattgcaga tgtttcatca ccaagggaac
    55261 gccccataaa taaagtagtg tccacagcag acaaacgaat tcagagtcgg aatgcaatcc
    55321 ataagcatat ctgcggatgc agatataaga aagaaaggtg attcctgttt ccgcccatga
    55381 gcctgtcacc agagaaaaga ctagaacgac cgctagtttt ccaagattat tcaataaaac
    55441 ttctaagaca aattttactt ggacatacac catatcttct tccgatttgt ctccgctgaa
    55501 taaggcttct aataatttgt tcacaacttt atctttcatc tcatacgtac ctccaccaca
    55561 aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc
    55621 aaaaccgtca tgatctccta aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg
    55681 acagttttga tacgaaaagt actttttatg acaagtttgt gctttttcga gtgatttatg
    55741 ttaaaaaatt actgacctta taaaaggtga tggagggatt cttatggaaa atgaacaatt
    55801 gtttgaagtc ggcgaaacat atgcatttaa atctgattta tttgaatcac caataaaagg
    55861 aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga
    55921 agacaatgat accgctcata atctgcactg gcagttagtt gtacgcaaat caagtacaat
    55981 agaagaataa aaacagataa aatgttgaga gcataaagag gttgtgagtc tcccgtttag
    56041 ctctgagtta caaggaataa tgttaaaaaa cagcttttca tgttttatga atattgcctt
    56101 gtgaccgagg tgttggcagg agaatactgt ttatttgaaa aaagggctgc gacaagactt
    56161 ttgtcacagc ctttattttc atgaatagga gttacttttg gctactttta gagaagtagc
    56221 ttttaaggag cagataaagt caaggctttt atttctataa atgaagtagt aaaatgaaga
    56281 acagatttta ttacaggaag gatgccgata attgtgatca ttgttgcagg aacgatcggt
    56341 gcagggaaaa gcacactaac tgaaatgctt gcccaagatt tagaaacaaa acctttttat
    56401 gagaacgtgg aagataatga agtattgccg ctattttatt ctaatccaga aaagtatact
    56461 tttttattac agattttctt tttgaacaaa cgctttttgg caatcaaaga tgctttcagc
    56521 catgatgaca acgtactaga ccgctccatc tacgaagata gtatgctgtt tcatttgaat
    56581 gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg
    56641 atgctgaagg agttagaaga aatttcgcca caaaagaaac cagatctact ggtttatatt
    56701 cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa gaggccggga atatgaacag
    56761 ctagaacaag atcctgaact ctattcctac tataaagaac tcaatcgacg atacgaagag
    56821 tggtatgaac agtttgatat atgtcccaaa atagtgatcg atggggacaa atatgatttc
    56881 gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa
    56941 atgacagaag aggcagatgc ccagtcgatc cgctacacta caaataaccc gaaagcaaag
    57001 ggaatcttta caactaaata atataaaaac taagcaaatt gctcactcgc agatatttct
    57061 ctgtgagcag catcttgctt agtttttttg attcattgag atcaaatagt gaaccgattc
    57121 gtatgataaa attgccataa atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg
    57181 gaaacgatca aaatcgaatg ggcgccaaac agatgatgac agaaaacaag gacaatagct
    57241 cctgcgataa aggcaagaac gacacttaga tagtggaaaa atcgatcaaa atctgctttt
    57301 tctttagaaa tacaataatc atataagttc gttgctaccc cttttaaatt tccagttgta
    57361 aataaattag tgtaggcacg cccgttgatt ttatcaaaag ctacccactg gatcgatgca
    57421 gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat ggacgggtgt caaactaaca
    57481 aagaaaaaaa tcactgcttc ataatataga acaaacaagc gccagaaatg tttttctttt
    57541 tttctaaaat aatcaattag atactttgtt agaagaatcc ctaagaaaaa gaaaagggta
    57601 gacaatactt ttttaccaac acttagccat tctccgtcaa atgcttggat gatcgataga
    57661 atgatattgc cagtttgagc agaggcaaaa gcaccatatt ggatataagt ataactgtcc
    57721 atggctcctc cgataaatgt cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc
    57781 tgcatttttt tcatcacctt ttatgaatat ttttgtcttc cttatcttag cataaacgat
    57841 tgaaaaaaaa aggaaaaatt ttcagaaaac taaaatcata tcgcttccca ttttcatttt
    57901 tagaaaggtc tgttagtata aagaaaaaat agacaacgaa actttaaata accaacttga
    57961 aataaacaaa ttcaaagaag aaagatggga aggaaatcaa tcagttgaat gaaaaagaaa
    58021 ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa gtataaaaag cttcaacaag
    58081 tctttcggct acaagaagaa atcattgcta cctatgaagc ggcttatggc gaagcacccg
    58141 gacttattga ggaagaagta caagtaaaag aaagtgaagg aaacagctct ttctttaaaa
    58201 tgatggagaa acgttcctat aatcgcttca atcaagaaca aaaagcagct atcacatatg
    58261 atatgaagaa acatcttcga atcatagcag gagctgggag cggcaaaacg caaacaatct
    58321 gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga cgaagaacgc atactaatga
    58381 tcacttttac aaggaacgct gcaaatgaac taaaaaaacg agtagataat ttcagccaga
    58441 gaaaaaccgc tgtccatatc ggaacattcc atagtatctt ttttaggata tacaatgaaa
    58501 tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca aggagacttt tcaaaggata
    58561 ctgcacaaaa agtgaatgct gttttgcaac agctgatacg taaatataat ctttatatat
    58621 ttgatcaata tggagaaaaa acgattgctt ctcggttaga ctattggcag aatatgaacc
    58681 tttcagtaga agaaatggtt cagttagtta aagaaaaata tgattccatt gataagaatg
    58741 cacgacagcc gatatcagaa cgactgtatg gattgctcac ggaactccaa gaacaaaaaa
    58801 gaagccagca attgctagag ttcaatgatg tactccaaaa tctgaaaagc gctttggaga
    58861 atgaggagat ccagcattat atcggccaaa aatatgatta tctattcatt gatgaatttc
    58921 aagatactaa tccacttcaa tgggaaatcg tgaaacggat gacaaaggaa aacaggatca
    58981 agttgattgt tgttggagat gatgatcaga gtatctatgg attcagagga tcagagccag
    59041 catacatcaa gaactttgaa aaagaatatc ctaccaaaac tcttttctta ttaactaatt
    59101 atcgttctcg ggcagctatt gtccaaggag caaatagatt gatcagctat aataaaaatg
    59161 atcggatacc aaaggcgatg attccagcac aaaaagaagc aggtgttatg gaagcctatt
    59221 tgttttctga tactaaagca gaaagtcagt ggctaatcaa acaaattcag acatttattc
    59281 aaaaaaatgg gaaatataag gaatcaatca tattataccg atcagcttca caaacgcagc
    59341 agcttgtcca atctctgctg aaaacgaata ttccttttgt actggaagca gatagtccat
    59401 atgaaggtat ctttggtatc aagtctttcc aacatttcta tcaaaaaatt gttcattggc
    59461 aaacagcact gaatctgcaa aagaaaaatc aggcctacca gcaattgctc cggcagctga
    59521 tggcagactg ctacctgaaa aaaagtgaat gcgatcaata cttttctcct aaatatcaaa
    59581 atatagccgt tgcagattat attttgtcga taagccccaa tttaaaatcg aaatctgcag
    59641 aaatccgaga gtttgaggaa gcgttgcgta aaacagacca gcatcaagaa atctattcgc
    59701 ttgttcaaag ctatcttcat ctgccacgta tagctaagga aatcgaccag agtgatcaag
    59761 aatggatacg agaagaagta aagcagcatg ctactttcag aagactccaa gccttatcgc
    59821 aagaaaccca agcgacagct aaagaattaa aaacccgctt gattgcttac agacaaggaa
    59881 aacttgatgc actatgtatc cagagcattc acaaaagtaa aggtcttagt taccgaaatg
    59941 tctttttgat tggttgtaac gaaggaagtt tgccttataa cggagcgaca gaaaagaaag
    60001 taatcgactt taatgaaata aaagcagaac cagtaacgac gatagaagaa gaacgaagac
    60061 tattttatgt tgcgatgact agagcaagaa atcgtctgta tctatgtgta cctcaaatga
    60121 aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga aaccgggagt cgtgtgaaaa
    60181 aaggaaagtg atagaagaga gggtgtgaca gaagtggtca gtttcaagaa agaagaagga
    60241 atgtacgaaa attgcttctc aaatttttgt gaaatttcag cttattttcg aagaagctat
    60301 ttctggaaca cagtttattc ggaaaaggg
//
LOCUS       DAZKAY010000005        59823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000005 DAZKAY010000000
VERSION     DAZKAY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.005"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8WP_000266"
     CDS             complement(451..1761)
                     /locus_tag="ACG8WP_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9026608.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8WP_000267"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8WP_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9026609.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG8WP_000268"
     CDS             complement(3431..4999)
                     /locus_tag="ACG8WP_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9026610.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG8WP_000269"
     CDS             complement(5003..5413)
                     /locus_tag="ACG8WP_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9026611.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG8WP_000270"
     CDS             complement(5410..6333)
                     /locus_tag="ACG8WP_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9026612.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG8WP_000271"
     CDS             complement(6330..7208)
                     /locus_tag="ACG8WP_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9026613.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG8WP_000272"
     CDS             complement(7195..8532)
                     /locus_tag="ACG8WP_000272"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9026614.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG8WP_000273"
     CDS             complement(8535..9638)
                     /locus_tag="ACG8WP_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9026615.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG8WP_000274"
     CDS             complement(9712..11220)
                     /locus_tag="ACG8WP_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9026616.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG8WP_000275"
     CDS             complement(11186..11878)
                     /locus_tag="ACG8WP_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9026617.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG8WP_000276"
     CDS             complement(11900..12874)
                     /locus_tag="ACG8WP_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026618.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG8WP_000277"
     CDS             13174..14046
                     /locus_tag="ACG8WP_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026619.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACG8WP_000278"
     CDS             complement(14504..16702)
                     /locus_tag="ACG8WP_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026620.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACG8WP_000279"
     CDS             16934..17926
                     /locus_tag="ACG8WP_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026621.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACG8WP_000280"
     CDS             17986..19407
                     /locus_tag="ACG8WP_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9026622.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACG8WP_000281"
     CDS             19400..20899
                     /locus_tag="ACG8WP_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9026623.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8WP_000282"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8WP_000282"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9026624.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACG8WP_000283"
     CDS             22491..23174
                     /locus_tag="ACG8WP_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9026625.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACG8WP_000284"
     CDS             23176..24012
                     /locus_tag="ACG8WP_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9026626.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACG8WP_000285"
     CDS             24023..25777
                     /locus_tag="ACG8WP_000285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026627.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACG8WP_000286"
     CDS             complement(26053..26394)
                     /locus_tag="ACG8WP_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9026628.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACG8WP_000287"
     CDS             complement(26462..28633)
                     /locus_tag="ACG8WP_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9026629.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACG8WP_000288"
     CDS             28844..29701
                     /locus_tag="ACG8WP_000288"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9026630.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACG8WP_000289"
     CDS             complement(29772..30629)
                     /locus_tag="ACG8WP_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9026631.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACG8WP_000290"
     CDS             complement(30614..33316)
                     /locus_tag="ACG8WP_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9026632.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACG8WP_000291"
     CDS             complement(33329..34618)
                     /locus_tag="ACG8WP_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9026633.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACG8WP_000292"
     CDS             34768..35814
                     /locus_tag="ACG8WP_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9026634.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACG8WP_000293"
     CDS             35816..37969
                     /locus_tag="ACG8WP_000293"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9026635.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="ACG8WP_000294"
     CDS             complement(38442..39893)
                     /locus_tag="ACG8WP_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9026636.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="ACG8WP_000295"
     CDS             complement(39890..41614)
                     /locus_tag="ACG8WP_000295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9026637.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="ACG8WP_000296"
     CDS             complement(41607..42221)
                     /locus_tag="ACG8WP_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9026638.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="ACG8WP_000297"
     CDS             complement(42566..44023)
                     /locus_tag="ACG8WP_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9026639.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="ACG8WP_000298"
     CDS             complement(44064..44984)
                     /locus_tag="ACG8WP_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026640.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="ACG8WP_000299"
     CDS             complement(44996..45943)
                     /locus_tag="ACG8WP_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026641.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="ACG8WP_000300"
     CDS             complement(46422..47141)
                     /locus_tag="ACG8WP_000300"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9026642.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="ACG8WP_000301"
     CDS             complement(47190..48836)
                     /locus_tag="ACG8WP_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9026643.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="ACG8WP_000302"
     CDS             49015..49605
                     /locus_tag="ACG8WP_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9026644.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="ACG8WP_000303"
     CDS             49697..51202
                     /locus_tag="ACG8WP_000303"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9026645.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG8WP_000304"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG8WP_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9026646.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="ACG8WP_000305"
     CDS             complement(52638..53156)
                     /locus_tag="ACG8WP_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026647.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="ACG8WP_000306"
     CDS             complement(53172..53498)
                     /locus_tag="ACG8WP_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9026648.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="ACG8WP_000307"
     CDS             complement(53511..55490)
                     /locus_tag="ACG8WP_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9026649.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="ACG8WP_000308"
     CDS             complement(55511..55825)
                     /locus_tag="ACG8WP_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9026650.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="ACG8WP_000309"
     CDS             55993..56286
                     /locus_tag="ACG8WP_000309"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9026651.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="ACG8WP_000310"
     CDS             complement(56364..57476)
                     /locus_tag="ACG8WP_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9026652.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..58477
                     /locus_tag="ACG8WP_000311"
                     /pseudo
     CDS             57629..58477
                     /locus_tag="ACG8WP_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(58830..>59823)
                     /locus_tag="ACG8WP_000312"
     CDS             complement(58830..>59823)
                     /locus_tag="ACG8WP_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFP9026653.1"
                     /translation="GGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLA
                     IDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKID
                     DQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELD
                     ATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENR
                     KVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17859 a        12242 c        10462 g        19260 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt
    59641 ggcgcttgag tttctaccag ctgatatgct cctggtgcta aaccgtcaat tgccagtttt
    59701 ccatcttctc ctgtagttag accggtttgt agcgtttctc cttctcggtt ctgtaactca
    59761 aaaacagctc cttgcaggat ttccccgctt tggtcatcga tctttgtcaa cacgacaccg
    59821 cct
//
LOCUS       DAZKAY010000006        59772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000006 DAZKAY010000000
VERSION     DAZKAY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.006"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..823)
                     /locus_tag="ACG8WP_000313"
     CDS             complement(<1..823)
                     /locus_tag="ACG8WP_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9026654.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="ACG8WP_000314"
     CDS             complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="ACG8WP_000314"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9026655.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3324..3503)
                     /locus_tag="ACG8WP_000315"
     CDS             complement(3324..3503)
                     /locus_tag="ACG8WP_000315"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9026656.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3550..5373)
                     /locus_tag="ACG8WP_000316"
     CDS             complement(3550..5373)
                     /locus_tag="ACG8WP_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9026657.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5651..7930
                     /gene="helD"
                     /locus_tag="ACG8WP_000317"
     CDS             5651..7930
                     /gene="helD"
                     /locus_tag="ACG8WP_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9026658.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8023..8670)
                     /locus_tag="ACG8WP_000318"
     CDS             complement(8023..8670)
                     /locus_tag="ACG8WP_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026659.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8849..9568)
                     /locus_tag="ACG8WP_000319"
     CDS             complement(8849..9568)
                     /locus_tag="ACG8WP_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026660.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9699..10850)
                     /locus_tag="ACG8WP_000320"
     CDS             complement(9699..10850)
                     /locus_tag="ACG8WP_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9026661.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11067..11807)
                     /locus_tag="ACG8WP_000321"
     CDS             complement(11067..11807)
                     /locus_tag="ACG8WP_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9026662.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11811..12134)
                     /gene="rsfS"
                     /locus_tag="ACG8WP_000322"
     CDS             complement(11811..12134)
                     /gene="rsfS"
                     /locus_tag="ACG8WP_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9026663.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12191..12784)
                     /gene="yqeK"
                     /locus_tag="ACG8WP_000323"
     CDS             complement(12191..12784)
                     /gene="yqeK"
                     /locus_tag="ACG8WP_000323"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9026664.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12768..13418)
                     /locus_tag="ACG8WP_000324"
     CDS             complement(12768..13418)
                     /locus_tag="ACG8WP_000324"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9026665.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13428..13739)
                     /gene="yhbY"
                     /locus_tag="ACG8WP_000325"
     CDS             complement(13428..13739)
                     /gene="yhbY"
                     /locus_tag="ACG8WP_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9026666.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13755..14864)
                     /gene="yqeH"
                     /locus_tag="ACG8WP_000326"
     CDS             complement(13755..14864)
                     /gene="yqeH"
                     /locus_tag="ACG8WP_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9026667.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14861..15388)
                     /locus_tag="ACG8WP_000327"
     CDS             complement(14861..15388)
                     /locus_tag="ACG8WP_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9026668.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15568..16353)
                     /locus_tag="ACG8WP_000328"
     CDS             complement(15568..16353)
                     /locus_tag="ACG8WP_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9026669.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16346..17215)
                     /gene="accD"
                     /locus_tag="ACG8WP_000329"
     CDS             complement(16346..17215)
                     /gene="accD"
                     /locus_tag="ACG8WP_000329"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9026670.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17216..18595)
                     /locus_tag="ACG8WP_000330"
     CDS             complement(17216..18595)
                     /locus_tag="ACG8WP_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9026671.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18603..19022)
                     /gene="fabZ"
                     /locus_tag="ACG8WP_000331"
     CDS             complement(18603..19022)
                     /gene="fabZ"
                     /locus_tag="ACG8WP_000331"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9026672.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19047..19523)
                     /gene="accB"
                     /locus_tag="ACG8WP_000332"
     CDS             complement(19047..19523)
                     /gene="accB"
                     /locus_tag="ACG8WP_000332"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9026673.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19526..20761)
                     /gene="fabF"
                     /locus_tag="ACG8WP_000333"
     CDS             complement(19526..20761)
                     /gene="fabF"
                     /locus_tag="ACG8WP_000333"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9026674.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20780..21517)
                     /gene="fabG"
                     /locus_tag="ACG8WP_000334"
     CDS             complement(20780..21517)
                     /gene="fabG"
                     /locus_tag="ACG8WP_000334"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9026675.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21529..22446)
                     /gene="fabD"
                     /locus_tag="ACG8WP_000335"
     CDS             complement(21529..22446)
                     /gene="fabD"
                     /locus_tag="ACG8WP_000335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9026676.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22553..22777)
                     /locus_tag="ACG8WP_000336"
     CDS             complement(22553..22777)
                     /locus_tag="ACG8WP_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9026677.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22830..23792)
                     /locus_tag="ACG8WP_000337"
     CDS             complement(22830..23792)
                     /locus_tag="ACG8WP_000337"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9026678.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23802..24242)
                     /locus_tag="ACG8WP_000338"
     CDS             complement(23802..24242)
                     /locus_tag="ACG8WP_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9026679.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24548..25513
                     /locus_tag="ACG8WP_000339"
     CDS             24548..25513
                     /locus_tag="ACG8WP_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9026680.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25570..25800)
                     /locus_tag="ACG8WP_000340"
     CDS             complement(25570..25800)
                     /locus_tag="ACG8WP_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9026681.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25886..26773)
                     /locus_tag="ACG8WP_000341"
     CDS             complement(25886..26773)
                     /locus_tag="ACG8WP_000341"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9026682.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26862..28085)
                     /locus_tag="ACG8WP_000342"
     CDS             complement(26862..28085)
                     /locus_tag="ACG8WP_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9026683.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28102..29148)
                     /locus_tag="ACG8WP_000343"
     CDS             complement(28102..29148)
                     /locus_tag="ACG8WP_000343"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9026684.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29312..31039)
                     /gene="ptsP"
                     /locus_tag="ACG8WP_000344"
     CDS             complement(29312..31039)
                     /gene="ptsP"
                     /locus_tag="ACG8WP_000344"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9026685.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31039..31305)
                     /locus_tag="ACG8WP_000345"
     CDS             complement(31039..31305)
                     /locus_tag="ACG8WP_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9026686.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31784..34018
                     /locus_tag="ACG8WP_000346"
     CDS             31784..34018
                     /locus_tag="ACG8WP_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9026687.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34166..34486
                     /locus_tag="ACG8WP_000347"
     CDS             34166..34486
                     /locus_tag="ACG8WP_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9026688.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34691..34915)
                     /locus_tag="ACG8WP_000348"
     CDS             complement(34691..34915)
                     /locus_tag="ACG8WP_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9026689.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35113..36693)
                     /locus_tag="ACG8WP_000349"
     CDS             complement(35113..36693)
                     /locus_tag="ACG8WP_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9026690.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37094..38470)
                     /locus_tag="ACG8WP_000350"
     CDS             complement(37094..38470)
                     /locus_tag="ACG8WP_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9026691.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38598..39686)
                     /locus_tag="ACG8WP_000351"
     CDS             complement(38598..39686)
                     /locus_tag="ACG8WP_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9026692.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39858..40292
                     /locus_tag="ACG8WP_000352"
     CDS             39858..40292
                     /locus_tag="ACG8WP_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9026693.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40370..40903)
                     /locus_tag="ACG8WP_000353"
     CDS             complement(40370..40903)
                     /locus_tag="ACG8WP_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9026694.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40997..42175)
                     /gene="mutY"
                     /locus_tag="ACG8WP_000354"
     CDS             complement(40997..42175)
                     /gene="mutY"
                     /locus_tag="ACG8WP_000354"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9026695.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42168..42965)
                     /gene="recX"
                     /locus_tag="ACG8WP_000355"
     CDS             complement(42168..42965)
                     /gene="recX"
                     /locus_tag="ACG8WP_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9026696.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43072..44454
                     /gene="rlmD"
                     /locus_tag="ACG8WP_000356"
     CDS             43072..44454
                     /gene="rlmD"
                     /locus_tag="ACG8WP_000356"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9026697.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44628..45206)
                     /locus_tag="ACG8WP_000357"
     CDS             complement(44628..45206)
                     /locus_tag="ACG8WP_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9026698.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45528..45998
                     /locus_tag="ACG8WP_000358"
     CDS             45528..45998
                     /locus_tag="ACG8WP_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9026699.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46106..47332)
                     /locus_tag="ACG8WP_000359"
     CDS             complement(46106..47332)
                     /locus_tag="ACG8WP_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9026700.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48086..48286)
                     /locus_tag="ACG8WP_000360"
     CDS             complement(48086..48286)
                     /locus_tag="ACG8WP_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9026701.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48420..49028)
                     /locus_tag="ACG8WP_000361"
     CDS             complement(48420..49028)
                     /locus_tag="ACG8WP_000361"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9026702.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49113..49583)
                     /locus_tag="ACG8WP_000362"
     CDS             complement(49113..49583)
                     /locus_tag="ACG8WP_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9026703.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49631..50545)
                     /locus_tag="ACG8WP_000363"
     CDS             complement(49631..50545)
                     /locus_tag="ACG8WP_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9026704.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50691..51494
                     /locus_tag="ACG8WP_000364"
     CDS             50691..51494
                     /locus_tag="ACG8WP_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9026705.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51672..52619
                     /locus_tag="ACG8WP_000365"
     CDS             51672..52619
                     /locus_tag="ACG8WP_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9026706.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52687..52980)
                     /locus_tag="ACG8WP_000366"
     CDS             complement(52687..52980)
                     /locus_tag="ACG8WP_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9026707.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53008..53673)
                     /locus_tag="ACG8WP_000367"
     CDS             complement(53008..53673)
                     /locus_tag="ACG8WP_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9026708.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53813..54445)
                     /locus_tag="ACG8WP_000368"
     CDS             complement(53813..54445)
                     /locus_tag="ACG8WP_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9026709.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54452..55519)
                     /gene="liaS"
                     /locus_tag="ACG8WP_000369"
     CDS             complement(54452..55519)
                     /gene="liaS"
                     /locus_tag="ACG8WP_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9026710.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55516..56247)
                     /gene="liaF"
                     /locus_tag="ACG8WP_000370"
     CDS             complement(55516..56247)
                     /gene="liaF"
                     /locus_tag="ACG8WP_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9026711.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56416..56895)
                     /gene="greA"
                     /locus_tag="ACG8WP_000371"
     CDS             complement(56416..56895)
                     /gene="greA"
                     /locus_tag="ACG8WP_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9026712.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57031..58206)
                     /gene="mltG"
                     /locus_tag="ACG8WP_000372"
     CDS             complement(57031..58206)
                     /gene="mltG"
                     /locus_tag="ACG8WP_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9026713.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58410..59579
                     /locus_tag="ACG8WP_000373"
     CDS             58410..59579
                     /locus_tag="ACG8WP_000373"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9026714.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        17325 a        12569 c        10095 g        19783 t
ORIGIN      
        1 ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga
       61 aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc
      121 gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg
      181 ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg
      241 ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt
      301 tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct
      361 taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc
      421 catataaaaa aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt
      481 cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt
      541 tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct
      601 gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg
      661 aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca
      721 ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag
      781 aataatcttc catcatactt tcaatacgac taattacgct cataagttcc tccacccttt
      841 taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata
      901 aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta
      961 ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta
     1021 ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct
     1081 tccatagcat caataaagat attcaaatct tctacagctg tttctttact tccataacga
     1141 cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc
     1201 ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct
     1261 acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca
     1321 tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct
     1381 ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg
     1441 ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc
     1501 tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt
     1561 ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa
     1621 tctagttcta attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg
     1681 tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa
     1741 tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca
     1801 tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca
     1861 cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg
     1921 atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga
     1981 gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac
     2041 atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt
     2101 attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg
     2161 atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct
     2221 ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct
     2281 tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat
     2341 acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat
     2401 aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg
     2461 aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct
     2521 actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt
     2581 tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct
     2641 tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct
     2701 ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga
     2761 gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata
     2821 cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct
     2881 aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca
     2941 gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc
     3001 ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct
     3061 tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg
     3121 ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc
     3181 atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc
     3241 tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag
     3301 aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct
     3361 tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga
     3421 acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact
     3481 aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac
     3541 tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt
     3601 ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc
     3661 gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat
     3721 gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt
     3781 tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt
     3841 agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt
     3901 cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt
     3961 cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat
     4021 cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt
     4081 tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt
     4141 tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc
     4201 agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat
     4261 tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt
     4321 gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag
     4381 aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc
     4441 tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat
     4501 cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa
     4561 agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa
     4621 tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt
     4681 gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc
     4741 ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat
     4801 ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt
     4861 ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac
     4921 tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat
     4981 ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt
     5041 gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact
     5101 atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa
     5161 tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt
     5221 cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc
     5281 aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag
     5341 caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt
     5401 agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag
     5461 cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat
     5521 tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa
     5581 aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag
     5641 caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact
     5701 aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa
     5761 tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta
     5821 tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct
     5881 tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc
     5941 ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt
     6001 aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta
     6061 ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga
     6121 tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac
     6181 gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca
     6241 tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac
     6301 aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca
     6361 acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct
     6421 attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga
     6481 aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc
     6541 tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca
     6601 agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac
     6661 aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga
     6721 atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc
     6781 tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca
     6841 gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa
     6901 tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt
     6961 acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac
     7021 gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat
     7081 cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga
     7141 attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac
     7201 ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca
     7261 agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa
     7321 tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg
     7381 tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt
     7441 tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct
     7501 tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag cagataccat
     7561 tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga
     7621 agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat
     7681 tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa
     7741 aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca
     7801 gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt
     7861 tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa
     7921 agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct ttttatttac
     7981 aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta tatataaatc
     8041 caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat
     8101 gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat
     8161 caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg
     8221 atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac
     8281 tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg
     8341 tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa
     8401 ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat
     8461 cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac
     8521 atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga
     8581 acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc
     8641 aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc
     8701 atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa
     8761 taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga
     8821 ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat
     8881 gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt
     8941 ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag
     9001 tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa
     9061 cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt
     9121 attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca
     9181 tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta
     9241 cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc
     9301 ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt
     9361 tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga
     9421 cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga
     9481 tttcaatccc aaattttgca taaatccggc tattattagc atctttagca gttttctttg
     9541 ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc
     9601 ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt
     9661 tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc
     9721 ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact tctaactaac
     9781 agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt
     9841 tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt
     9901 tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg
     9961 atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca
    10021 gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct
    10081 gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc
    10141 caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct
    10201 attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct
    10261 gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg
    10321 tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa
    10381 aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa
    10441 ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg
    10501 acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa
    10561 ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac
    10621 tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg taaagcttca
    10681 ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc
    10741 attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga
    10801 tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc
    10861 cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa
    10921 aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca
    10981 ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt
    11041 cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt
    11101 gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg
    11161 ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca
    11221 tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt
    11281 tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct
    11341 tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca
    11401 aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc
    11461 tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt
    11521 ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca
    11581 tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta
    11641 tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata
    11701 ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt
    11761 tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac
    11821 cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt
    11881 cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt
    11941 ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg
    12001 atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa
    12061 tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa
    12121 tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa
    12181 tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa
    12241 tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt
    12301 cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg
    12361 gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag
    12421 tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta
    12481 caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat
    12541 caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat
    12601 catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag
    12661 ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct
    12721 gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat
    12781 tcatacaacc cttcattttg aatataatca attactttat ctggaacaag gtagcggata
    12841 gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca
    12901 tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca
    12961 aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata
    13021 tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata
    13081 gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa
    13141 ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt
    13201 ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa
    13261 ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg
    13321 cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat
    13381 tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt
    13441 acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca
    13501 cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct
    13561 tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa
    13621 gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc
    13681 ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata
    13741 attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc
    13801 cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg
    13861 tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca
    13921 tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt
    13981 tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga
    14041 ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc
    14101 agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta
    14161 cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc
    14221 ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg
    14281 attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg
    14341 acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct
    14401 aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc tactggacga
    14461 attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat
    14521 ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg
    14581 tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat
    14641 gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca
    14701 tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa
    14761 gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt
    14821 tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc
    14881 agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat
    14941 taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc
    15001 ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat
    15061 catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg
    15121 ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat
    15181 tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca
    15241 actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata
    15301 ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca
    15361 tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc
    15421 acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat
    15481 actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc
    15541 acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc
    15601 cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt
    15661 cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac
    15721 tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc
    15781 ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac
    15841 agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc
    15901 gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc
    15961 gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata
    16021 tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat
    16081 cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt
    16141 ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg
    16201 agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc
    16261 gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc
    16321 taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca
    16381 attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa
    16441 aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg
    16501 tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa
    16561 tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag
    16621 ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct
    16681 gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga
    16741 ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg
    16801 ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct
    16861 gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt
    16921 taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc
    16981 attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa
    17041 aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat
    17101 gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt
    17161 tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg
    17221 cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg
    17281 tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt
    17341 tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt
    17401 caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga
    17461 tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca
    17521 gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa
    17581 tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct
    17641 caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta
    17701 ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat
    17761 ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag
    17821 cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca
    17881 gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac
    17941 caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca
    18001 aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct
    18061 gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg
    18121 ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag
    18181 ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg
    18241 cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat
    18301 tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat
    18361 gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg
    18421 aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg
    18481 cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac
    18541 gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc
    18601 acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg
    18661 attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag
    18721 gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc
    18781 gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct
    18841 tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc
    18901 tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact
    18961 cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc
    19021 attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca
    19081 tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta
    19141 gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact
    19201 tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc
    19261 acgattggcg aaacaacctc gattccatca aatactgccg attctttttc tttcattatt
    19321 tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt
    19381 tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca
    19441 aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat
    19501 tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat
    19561 tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt
    19621 cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg
    19681 ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc
    19741 ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac
    19801 ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt
    19861 gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca
    19921 ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt
    19981 ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta
    20041 aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt
    20101 caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa
    20161 atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag
    20221 aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag
    20281 ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg
    20341 ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat
    20401 gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga
    20461 cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta
    20521 aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa
    20581 aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag
    20641 catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc
    20701 tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca
    20761 tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt
    20821 gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt
    20881 gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa
    20941 cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag
    21001 ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat
    21061 aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta atgatggccc
    21121 cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca
    21181 aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat
    21241 ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt
    21301 tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta
    21361 caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa
    21421 tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac
    21481 tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt atagtattgc
    21541 tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat
    21601 ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc
    21661 gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc
    21721 aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg
    21781 aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg
    21841 ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc
    21901 tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat
    21961 ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca
    22021 gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc
    22081 tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa
    22141 ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc
    22201 atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat
    22261 agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc
    22321 catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc
    22381 atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt
    22441 tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc
    22501 cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag
    22561 tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga
    22621 ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag
    22681 cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt
    22741 cttctgcgat gatttcttga attttttcga ataccataat aatatattcc tccgatattg
    22801 tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt
    22861 aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca
    22921 ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta
    22981 tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata
    23041 attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct
    23101 tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc
    23161 aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca
    23221 gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc
    23281 tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta
    23341 cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt
    23401 gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca
    23461 gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct
    23521 acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta
    23581 gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc
    23641 tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca
    23701 gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg
    23761 gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag
    23821 aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg
    23881 tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt
    23941 ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg
    24001 acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat
    24061 agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg
    24121 tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt
    24181 gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc
    24241 atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct
    24301 taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa
    24361 gattttactc atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa
    24421 tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc
    24481 cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg
    24541 agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat
    24601 gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt
    24661 aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca
    24721 tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg
    24781 tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta
    24841 taattatgta catattacca ataagggatt tgtattaaca gatactccta cggctcgaac
    24901 atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac
    24961 ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga
    25021 cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg
    25081 aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa
    25141 tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg
    25201 gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc
    25261 agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat
    25321 gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc
    25381 tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg
    25441 caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa
    25501 gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta
    25561 tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa
    25621 ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata
    25681 agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc
    25741 tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat
    25801 cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt
    25861 gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc
    25921 accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt
    25981 catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt
    26041 ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt
    26101 cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa
    26161 gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg
    26221 tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt
    26281 tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt
    26341 gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta
    26401 agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg
    26461 caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga
    26521 tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac
    26581 ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga
    26641 tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt
    26701 cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa
    26761 aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat
    26821 acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt
    26881 aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca
    26941 tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa
    27001 atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa
    27061 tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga
    27121 tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc
    27181 gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc
    27241 gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct
    27301 tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg
    27361 ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct
    27421 tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga
    27481 agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat
    27541 ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt
    27601 aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat
    27661 ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg
    27721 tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc
    27781 atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt
    27841 tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa
    27901 gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga
    27961 tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt
    28021 ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt
    28081 ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga
    28141 gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat
    28201 acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt
    28261 aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct
    28321 atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc
    28381 taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc
    28441 tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt
    28501 ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac
    28561 atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat
    28621 gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag
    28681 tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc
    28741 gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt
    28801 tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt
    28861 ttttacgata ggagaaagct ggttcgatct gataaaggaa tttcggaaat cttcctctgt
    28921 agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg
    28981 actatttatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt
    29041 ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc
    29101 agattttgca agcatctttt cactttcaaa atataataat accttcacct tacgtccccc
    29161 ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg
    29221 tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt
    29281 ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa
    29341 cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg
    29401 ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga
    29461 actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc
    29521 cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac
    29581 gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg
    29641 ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg
    29701 caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg
    29761 tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt
    29821 ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat
    29881 gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag
    29941 ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca
    30001 gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt
    30061 ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg
    30121 gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat
    30181 ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt
    30241 cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat
    30301 ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc
    30361 cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg
    30421 tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc
    30481 caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg
    30541 gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag
    30601 taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct
    30661 cttgcatata agcattatct tccattgctt cgaacatacc gatatacata tccgttactt
    30721 cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta
    30781 ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac
    30841 ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg
    30901 cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta
    30961 aatccggttg aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc
    31021 cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc
    31081 tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc
    31141 tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc
    31201 taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc
    31261 gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct
    31321 ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac
    31381 aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc
    31441 attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac
    31501 attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta
    31561 tcattctttc ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt
    31621 atttttagta taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta
    31681 cctcataaat ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta
    31741 aaatatggat gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg
    31801 cggcaagaat gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact
    31861 cgattactgt cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa
    31921 tatggcccaa aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc
    31981 tcgccaaatg caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa
    32041 caacaacttc aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata
    32101 cggaatcaat atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga
    32161 tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt
    32221 gattggagaa ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt
    32281 agatggtgat gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc
    32341 tttagtccaa ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga
    32401 agagatccgt caagcagaaa atgtgatcct attcatagat gaagttcacg aaatcgtcgg
    32461 tgctggttct gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc
    32521 tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa
    32581 agacgctgct ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga
    32641 aacgatcagt attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata
    32701 tactgatgag gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt
    32761 cttgccggat aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat
    32821 ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt
    32881 tttagcttct cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa
    32941 acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa
    33001 agatatggaa aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa
    33061 agaacagaca caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga
    33121 taacgctgta gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca
    33181 gaatcgtcca atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct
    33241 tgcgaaacaa cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat
    33301 gtctgaatat atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt
    33361 cggatacgaa gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt
    33421 cttgttagat gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt
    33481 agatgatggt cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat
    33541 cattatgaca agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc
    33601 acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt
    33661 tctgaaccgt tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac
    33721 aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat
    33781 cgaagttcct acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg
    33841 tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta
    33901 tcttgatcac ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat
    33961 cgttacttct aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat
    34021 ccagttttat agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga
    34081 aaagtcaaaa tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta
    34141 aaaatacata aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg
    34201 tcttgtatta aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg
    34261 aaataccact attgtctaca ctgaagcccc tgaacataat gaaattctgc tgatcaatga
    34321 caaacgtaaa atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat
    34381 caaagacgcc gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga
    34441 aatctcgatt ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa attttgaaca
    34501 taataattaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    34561 tataaacggc gaaagcagaa gcaactcctt cggaaataag ctgaaattca cgaaaattgc
    34621 ttttcaaatt tttgtgaatt ccagcttatt tcttggagtt aaacgcttct gtctcagcct
    34681 ctttttcttt ctagcttttt gatttttctc ttttcttttg tacttttctc ttttcatttt
    34741 cagttctacg cgtgtcttta tttttaactt tttcctcttt atggatgatt gatcgtactt
    34801 tttgtgcaat ttgctcgttt ttgtcccagt cttttacatc taataatcga cataatcgta
    34861 acatctcttc ttttttctgt ttttttcttt cacgtctttg ccattctgtt tccatgtgtc
    34921 acactctcct ttctttaatc atatgtacgc aaaataattt ttatattttt ttgattagac
    34981 atttctatga ttttaatata gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa
    35041 caaaatatga gaggctgttt ttgtttggct attcttatta cttgatgcag ggcagcttgt
    35101 gtcaagactt ctttacatca aactttttaa ttttacattg ggatatttat ccgcaaacca
    35161 gcgttctgca aattggttct cgaataagaa caacggttgg tcgaaacggt cacgagccaa
    35221 gatattgcga cttgaactca tcttctcatc caaatcttct ggatcgatcc agcgagcgat
    35281 tttgtgtccc attggagaca tgattacttc ggcgttatac tcatttaaca tacgatattg
    35341 gaatacttcg aattgtagct gaccaacagc tccaatgata tattcttccg ttagatatgt
    35401 tttataaagc tgaatcgctc cttcttgaac taattgatag attcctttgt ggaaggattt
    35461 ttgtttcatg acattttttg cagtcacttt cataaacagc tctggagtaa atgaagggag
    35521 ttcttcataa gcaactttta gttttccttc gaacaatgta tccccaatct gataattgcc
    35581 tgtatcatat acaccgatga tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc
    35641 tgccataaat tgtgtgacat tgcttaattt cagtttttta tttgtacgtt ccaaccagac
    35701 atccattcct cgttcgaatg ttccagaaca gattcggaca aaagcgattc ggtcacgatg
    35761 ggctggattc atatttgctt ggatcttgaa tacgaaacct gaaaattctt cctcataagg
    35821 acttaccatc tgatcatctt gtgttttgtg tccataagga gctggtgcca attcaacgaa
    35881 agtctctaaa aaagtctgta caccgaagtt agtcaatgca gaaccaaaaa atactggtgt
    35941 ctgatcgcca cgagcaattt tttcttgatt aaatgcatct ccagcctctt tgattagttc
    36001 tacttcttct aagacttgtt catataagga attttcagtt agaggatgat tttcgggaag
    36061 cagtccatct tcatccaatt ctactaatcg ttcatcctga taattttccg gacgataaaa
    36121 ctctacacga ttatggtaga tatcataaag accttctagc cctttaccca taccgattgg
    36181 ccaattcatc ggataagatt cgatatctaa tagctcttct aattcttcta acaattccaa
    36241 tggttcacga ccatcacgat ctagtttgtt gataaaagta aatatcggga tgccacgttt
    36301 ttttacaact tggaataatt tctttgtctg tgcttcgatc ccttttgcac tatcgattac
    36361 catgaccgca ctatccacgg ccatcaaagt acgataagta tcttcagaaa aatcttcatg
    36421 tcctggtgta tccaagatat ttactcgttt cccttgataa tcaaactgca taacagaact
    36481 agtaacagaa atcccacgtt gtttctcgat ttccatccag tctgatttgg caaaattacc
    36541 tgtttttttg ccttttacgg tcccggcttg acggatagcc ccaccaaaca atagtaattg
    36601 ttctgtaatc gttgttttcc cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt
    36661 gtccacttgt tgctttaatt ttggattatt cataacctaa ctccatttct tccattcaat
    36721 tatttatatt ttctctaggc acagattgtc cgatctctta aagaaagaca atttcatata
    36781 gggctgtgac aaaagccgtg tagcagtctc tttttccgag taaacggcgt tctagagaca
    36841 gcctatcagc aataagccca cccaagccaa aaatacgaaa agctgttttt gcttggctgc
    36901 tcttattact taatgcagga tagctttttc acaacctttg tcaactacta caactgaatc
    36961 agtatagcat ggattcagcg aatgaaaaag ccaccaaacc ataagagtca aatatttttt
    37021 tgtcagctga ctaaaagaaa agacgtgttg cttgtttatt caagcaacac gtctttcata
    37081 accaagtttc caattatctt cttggctcgt catcttcgaa ttcttttctg aagtatcttc
    37141 gttcttcttc aggttccgct tctgtttctt ccggatcatg aaaaatgaca cggattttaa
    37201 ggattcgaga accttccatt tcttcagaaa ccagcgtaat attatcgaca tcaaacgata
    37261 acttttcgcc ttcatccgga atcatgccta atgctgtaat caaataacca gccatcgtat
    37321 ctacatcact catatgcaga tcactgtcaa aagcttcatt gaactcatca atcagcatac
    37381 gtccttggat gatatattcg tgttcatcga ttttttcata aagattctca acttcatctg
    37441 attcatcatc gatttctccg acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa
    37501 cgacgccgcc atattcatct aataaaatag ccatctgatt ctgcgtcttt ttcatttcat
    37561 ataacagatc atcaataaag accgtttcag gaacgaataa aggctcctgc atgattttct
    37621 tgatatctaa attatcaaag ccgaatttat gggcagcttt tagtaaattc tttgtatgaa
    37681 ggattccgac tactttatct ttgtcttcgt tataaacagg gattcttgaa tagttttcgg
    37741 ataaaacttc attcacattt tcttccacag tgtcgttgat atcaatcata aatgcatccg
    37801 ttcgtgggac catgacttct cgggcaactt tcgtatcaag agaaaaaacc ccttggagca
    37861 tttctaattc ttcattattc aatacgcctt cgttttcaag catataacgc atttcatctc
    37921 tggtcatttt tgaatcttca tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg
    37981 tggaagcaga aagcagccag acgaagggct tagctactac accaatcaag cgtatcagcc
    38041 ctgaagtgaa ctttgcgact tcttctgatt tatttaaagc aatacgttta gggtacaatt
    38101 ctccaaaaac aatcgataca taggtcaaaa tagccagcac aatgatattt gcaatatttt
    38161 ttgccgctgt gccgccacct aataccggag ctaaccgttc cgataaagta ttagctagtg
    38221 aagcaccaga caaaatgttg accaaagtga ttcctacttg gattgtcgat aggaaattat
    38281 ttggattctg caatattttt aaaagctttt gagatttcac atctccttca tcagcttttt
    38341 gctcgattcg attcttattg atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat
    38401 tgatcaatgt caaaacgatc aataataaaa tctgcgctaa cagcgactga ctctcagggt
    38461 cagcattcat aatcgcattc tctccttata aaattaattt ttagtttacg atagaaaaaa
    38521 acgtggcact atagccacgt taaaatggta tcacaaagca attttctttg caataaaaac
    38581 aagtgtacta aaactaccta cgaggtcgta gcaattttct tcttgttttc attttgcttg
    38641 tcttctttga cgattttgat tatataggaa atgatcgtct tgcagattgc gtaaaaagga
    38701 acacctaaga agataccaag caatccggca atatttccag ctactagaag aacgataatg
    38761 atcgttaatg gatggatttt cagtgatttc ccaattacat taggataaat gatattccca
    38821 tccaactgtt ggacgattag cacaaccaca caacaaagta atgcttttac cggatcattg
    38881 aagactgtta ctagaaatgc aggtgctagt cctagataag gtcctaaata tggaattagg
    38941 tttgtcaatc ccgcaatgac cccaaataag aaagcataat ccacaccaat taccatatat
    39001 cctaaaaatg tgaaggtacc aacgaataaa cattcgattg cttgtccgct aatgtaatta
    39061 gccaatgttt tattcagttg ccccaataac tccacaattt ggtttcttct tttttctggg
    39121 aaaaactgtt ggatgtttgg cactagtttt tctccgtctt tcaacatata gaacaagatg
    39181 aatggtgctg taatgattac tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa
    39241 ctattggata ctccactaag gaattgctga atgattgtcc cgtaagaaat gtctaattgc
    39301 tcaaaatatt tattgatatc tacttccttg aacaaaggca attcggctac ctcttttaac
    39361 cagctttcta cctgtttgat gaaatcaggc atattagatg ccaatgagga aatttgctgt
    39421 actagacttg ggatgacact aagtaaaatc caaataattg ctgcaactaa aaggagcagc
    39481 acaattgcga cagcccatat tcttttgatt tttgctttca tcaacagctt gacaatagga
    39541 ttcaataaat agtaaagaaa gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat
    39601 gtcccgatcg gttcgaaaat aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg
    39661 atcaataatt caagcgacca aaacattagt ttagactgtc taagtttttc aaacaataga
    39721 tttcctcctt tctttcttac tagtatatat tatattcgtt aaaaattctt ttattctttt
    39781 tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat actatacgaa
    39841 aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc aatatttact
    39901 tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg cctttgtctc
    39961 gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca gataaaaaag
    40021 aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga
    40081 tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt gaagaagcag
    40141 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
    40201 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
    40261 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
    40321 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
    40381 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
    40441 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
    40501 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
    40561 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
    40621 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
    40681 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
    40741 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
    40801 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
    40861 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
    40921 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
    40981 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
    41041 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
    41101 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
    41161 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
    41221 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
    41281 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
    41341 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
    41401 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
    41461 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
    41521 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
    41581 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
    41641 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
    41701 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
    41761 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
    41821 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
    41881 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
    41941 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
    42001 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
    42061 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
    42121 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
    42181 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
    42241 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
    42301 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
    42361 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
    42421 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
    42481 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
    42541 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
    42601 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
    42661 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
    42721 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
    42781 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
    42841 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
    42901 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
    42961 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
    43021 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
    43081 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
    43141 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
    43201 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
    43261 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
    43321 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
    43381 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
    43441 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
    43501 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
    43561 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
    43621 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
    43681 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
    43741 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
    43801 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
    43861 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
    43921 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
    43981 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
    44041 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
    44101 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
    44161 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
    44221 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
    44281 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
    44341 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
    44401 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
    44461 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
    44521 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
    44581 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
    44641 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
    44701 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
    44761 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
    44821 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
    44881 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
    44941 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
    45001 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
    45061 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
    45121 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
    45181 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
    45241 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
    45301 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
    45361 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
    45421 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
    45481 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
    45541 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
    45601 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
    45661 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
    45721 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
    45781 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
    45841 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
    45901 ggctgcatca tcgcctggca atgtggctca tgattcctta ttatttatgg gtactattcg
    45961 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
    46021 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
    46081 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
    46141 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
    46201 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
    46261 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
    46321 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
    46381 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
    46441 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
    46501 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
    46561 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
    46621 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
    46681 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
    46741 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
    46801 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
    46861 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
    46921 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
    46981 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
    47041 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
    47101 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
    47161 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
    47221 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
    47281 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
    47341 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
    47401 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
    47461 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
    47521 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
    47581 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
    47641 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
    47701 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
    47761 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
    47821 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
    47881 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
    47941 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
    48001 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
    48061 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
    48121 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
    48181 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
    48241 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
    48301 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
    48361 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
    48421 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
    48481 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
    48541 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
    48601 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
    48661 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
    48721 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
    48781 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
    48841 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
    48901 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
    48961 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
    49021 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
    49081 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
    49141 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
    49201 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
    49261 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
    49321 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
    49381 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
    49441 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
    49501 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
    49561 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
    49621 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
    49681 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
    49741 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
    49801 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
    49861 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
    49921 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
    49981 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
    50041 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
    50101 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    50161 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    50221 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    50281 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    50341 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    50401 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    50461 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    50521 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    50581 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    50641 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    50701 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    50761 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    50821 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    50881 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    50941 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    51001 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    51061 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    51121 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    51181 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    51241 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    51301 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    51361 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    51421 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    51481 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    51541 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    51601 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    51661 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    51721 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    51781 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    51841 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    51901 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    51961 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    52021 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    52081 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    52141 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    52201 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    52261 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    52321 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    52381 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    52441 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    52501 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    52561 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    52621 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    52681 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    52741 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    52801 atcaatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    52861 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    52921 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    52981 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    53041 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    53101 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    53161 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    53221 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    53281 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    53341 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    53401 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    53461 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    53521 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    53581 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    53641 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    53701 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    53761 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    53821 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    53881 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    53941 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    54001 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    54061 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    54121 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    54181 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    54241 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    54301 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    54361 cttcaatgtc ttcttgaata gataaataaa aagagacgcc taagcgcacc atttcatggt
    54421 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    54481 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    54541 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    54601 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    54661 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    54721 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    54781 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    54841 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    54901 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    54961 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    55021 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    55081 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    55141 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    55201 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    55261 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    55321 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    55381 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    55441 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    55501 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    55561 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    55621 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    55681 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    55741 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    55801 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    55861 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    55921 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    55981 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    56041 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    56101 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    56161 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    56221 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    56281 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    56341 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    56401 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    56461 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    56521 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    56581 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    56641 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    56701 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    56761 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    56821 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    56881 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    56941 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    57001 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    57061 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    57121 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    57181 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    57241 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    57301 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    57361 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    57421 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    57481 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    57541 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    57601 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    57661 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    57721 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    57781 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    57841 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    57901 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    57961 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    58021 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    58081 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    58141 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    58201 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    58261 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    58321 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    58381 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    58441 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    58501 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    58561 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    58621 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    58681 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    58741 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    58801 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    58861 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    58921 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    58981 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    59041 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    59101 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    59161 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    59221 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    59281 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    59341 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    59401 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    59461 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    59521 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    59581 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    59641 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    59701 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    59761 gatttctctt ta
//
LOCUS       DAZKAY010000007        58549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000007 DAZKAY010000000
VERSION     DAZKAY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.007"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ACG8WP_000374"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ACG8WP_000374"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9026715.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ACG8WP_000375"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ACG8WP_000375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9026716.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGWD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ACG8WP_000376"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ACG8WP_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9026717.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="ACG8WP_000377"
     CDS             complement(4503..5270)
                     /locus_tag="ACG8WP_000377"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9026718.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ACG8WP_000378"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ACG8WP_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9026719.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="ACG8WP_000379"
     CDS             6383..6781
                     /locus_tag="ACG8WP_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9026720.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7018..7560
                     /locus_tag="ACG8WP_000380"
     CDS             7018..7560
                     /locus_tag="ACG8WP_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9026721.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ACG8WP_000381"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ACG8WP_000381"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9026722.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="ACG8WP_000382"
     CDS             complement(8346..8573)
                     /locus_tag="ACG8WP_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9026723.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ACG8WP_000383"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ACG8WP_000383"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9026724.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="ACG8WP_000384"
     CDS             complement(9108..9821)
                     /locus_tag="ACG8WP_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9026725.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="ACG8WP_000385"
     CDS             complement(9824..10720)
                     /locus_tag="ACG8WP_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9026726.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="ACG8WP_000386"
     CDS             complement(10878..11720)
                     /locus_tag="ACG8WP_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9026727.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="ACG8WP_000387"
     CDS             11852..12505
                     /locus_tag="ACG8WP_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9026728.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="ACG8WP_000388"
     CDS             complement(12568..13095)
                     /locus_tag="ACG8WP_000388"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9026729.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="ACG8WP_000389"
     CDS             complement(13115..14062)
                     /locus_tag="ACG8WP_000389"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9026730.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15957)
                     /locus_tag="ACG8WP_000390"
     CDS             complement(14074..15957)
                     /locus_tag="ACG8WP_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9026731.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(16120..18198)
                     /locus_tag="ACG8WP_000391"
     CDS             complement(16120..18198)
                     /locus_tag="ACG8WP_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9026732.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(18211..18486)
                     /locus_tag="ACG8WP_000392"
     CDS             complement(18211..18486)
                     /locus_tag="ACG8WP_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026733.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(18848..19302)
                     /locus_tag="ACG8WP_000393"
                     /pseudo
     CDS             complement(18848..19302)
                     /locus_tag="ACG8WP_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19456..20664)
                     /locus_tag="ACG8WP_000394"
     CDS             complement(19456..20664)
                     /locus_tag="ACG8WP_000394"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9026734.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(20776..21108)
                     /locus_tag="ACG8WP_000395"
     CDS             complement(20776..21108)
                     /locus_tag="ACG8WP_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9026735.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            21256..22131
                     /locus_tag="ACG8WP_000396"
     CDS             21256..22131
                     /locus_tag="ACG8WP_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9026736.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(22213..22794)
                     /locus_tag="ACG8WP_000397"
     CDS             complement(22213..22794)
                     /locus_tag="ACG8WP_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9026737.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(22800..24059)
                     /locus_tag="ACG8WP_000398"
     CDS             complement(22800..24059)
                     /locus_tag="ACG8WP_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9026738.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(24061..25107)
                     /locus_tag="ACG8WP_000399"
     CDS             complement(24061..25107)
                     /locus_tag="ACG8WP_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026739.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(25342..25617)
                     /locus_tag="ACG8WP_000400"
     CDS             complement(25342..25617)
                     /locus_tag="ACG8WP_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9026740.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(25860..27170)
                     /gene="der"
                     /locus_tag="ACG8WP_000401"
     CDS             complement(25860..27170)
                     /gene="der"
                     /locus_tag="ACG8WP_000401"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9026741.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(27352..28581)
                     /gene="rpsA"
                     /locus_tag="ACG8WP_000402"
     CDS             complement(27352..28581)
                     /gene="rpsA"
                     /locus_tag="ACG8WP_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9026742.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(28716..29396)
                     /gene="cmk"
                     /locus_tag="ACG8WP_000403"
     CDS             complement(28716..29396)
                     /gene="cmk"
                     /locus_tag="ACG8WP_000403"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9026743.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(29445..30080)
                     /locus_tag="ACG8WP_000404"
     CDS             complement(29445..30080)
                     /locus_tag="ACG8WP_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9026744.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(30146..31549)
                     /locus_tag="ACG8WP_000405"
     CDS             complement(30146..31549)
                     /locus_tag="ACG8WP_000405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9026745.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(31536..32567)
                     /locus_tag="ACG8WP_000406"
     CDS             complement(31536..32567)
                     /locus_tag="ACG8WP_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026746.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            32598..32819
                     /locus_tag="ACG8WP_000407"
     CDS             32598..32819
                     /locus_tag="ACG8WP_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9026747.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(32984..33721)
                     /locus_tag="ACG8WP_000408"
     CDS             complement(32984..33721)
                     /locus_tag="ACG8WP_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9026748.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(33874..35214)
                     /locus_tag="ACG8WP_000409"
     CDS             complement(33874..35214)
                     /locus_tag="ACG8WP_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9026749.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(35451..36974)
                     /locus_tag="ACG8WP_000410"
     CDS             complement(35451..36974)
                     /locus_tag="ACG8WP_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9026750.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(37352..37963)
                     /locus_tag="ACG8WP_000411"
     CDS             complement(37352..37963)
                     /locus_tag="ACG8WP_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9026751.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(38086..38211)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(38293..39009)
                     /locus_tag="ACG8WP_000412"
     CDS             complement(38293..39009)
                     /locus_tag="ACG8WP_000412"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9026752.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(39015..39584)
                     /gene="scpB"
                     /locus_tag="ACG8WP_000413"
     CDS             complement(39015..39584)
                     /gene="scpB"
                     /locus_tag="ACG8WP_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9026753.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(39568..40338)
                     /locus_tag="ACG8WP_000414"
     CDS             complement(39568..40338)
                     /locus_tag="ACG8WP_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9026754.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(40335..40706)
                     /locus_tag="ACG8WP_000415"
     CDS             complement(40335..40706)
                     /locus_tag="ACG8WP_000415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9026755.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(40725..41612)
                     /gene="xerD"
                     /locus_tag="ACG8WP_000416"
     CDS             complement(40725..41612)
                     /gene="xerD"
                     /locus_tag="ACG8WP_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9026756.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(41708..42181)
                     /locus_tag="ACG8WP_000417"
     CDS             complement(41708..42181)
                     /locus_tag="ACG8WP_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9026757.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(42286..43149)
                     /locus_tag="ACG8WP_000418"
     CDS             complement(42286..43149)
                     /locus_tag="ACG8WP_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9026758.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(43345..45444)
                     /locus_tag="ACG8WP_000419"
     CDS             complement(43345..45444)
                     /locus_tag="ACG8WP_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9026759.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(45664..46773)
                     /gene="rpoD"
                     /locus_tag="ACG8WP_000420"
     CDS             complement(45664..46773)
                     /gene="rpoD"
                     /locus_tag="ACG8WP_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9026760.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(46797..48638)
                     /gene="dnaG"
                     /locus_tag="ACG8WP_000421"
     CDS             complement(46797..48638)
                     /gene="dnaG"
                     /locus_tag="ACG8WP_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9026761.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(48820..51135)
                     /locus_tag="ACG8WP_000422"
     CDS             complement(48820..51135)
                     /locus_tag="ACG8WP_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9026762.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(51320..52180)
                     /locus_tag="ACG8WP_000423"
     CDS             complement(51320..52180)
                     /locus_tag="ACG8WP_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9026763.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(52239..52871)
                     /locus_tag="ACG8WP_000424"
                     /pseudo
     CDS             complement(52239..52871)
                     /locus_tag="ACG8WP_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            53038..53799
                     /locus_tag="ACG8WP_000425"
     CDS             53038..53799
                     /locus_tag="ACG8WP_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9026764.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            53825..56416
                     /gene="mprF"
                     /locus_tag="ACG8WP_000426"
     CDS             53825..56416
                     /gene="mprF"
                     /locus_tag="ACG8WP_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9026765.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(56604..57791)
                     /gene="tuf"
                     /locus_tag="ACG8WP_000427"
     CDS             complement(56604..57791)
                     /gene="tuf"
                     /locus_tag="ACG8WP_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9026766.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            58159..58515
                     /locus_tag="ACG8WP_000428"
     CDS             58159..58515
                     /locus_tag="ACG8WP_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9026767.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        16155 a        12305 c        10077 g        20012 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatcccacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat
    16081 atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg gactcgtttt
    16141 ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt
    16201 ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt
    16261 ttcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt
    16321 tttcgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat
    16381 ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat
    16441 atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct
    16501 gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatatc ttctttgcca
    16561 aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc
    16621 gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta
    16681 aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg
    16741 ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa
    16801 atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc
    16861 taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac
    16921 ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag
    16981 cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa
    17041 caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat
    17101 cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa
    17161 ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa
    17221 tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg
    17281 atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat
    17341 aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga
    17401 caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc
    17461 cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg
    17521 aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa
    17581 atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact
    17641 agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc
    17701 aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa
    17761 aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc
    17821 atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa
    17881 caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa
    17941 gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct
    18001 aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg
    18061 tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat
    18121 cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac
    18181 taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa
    18241 aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttccc
    18301 ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc
    18361 caactgacga tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc
    18421 atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc
    18481 tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga
    18541 tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca
    18601 acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt
    18661 ttctgacaaa aggatgttta ctttcccatt cctgcctttc ttgttctttt atcacaacaa
    18721 aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga
    18781 agagacagcc tatcagtagt aataagacac atctctttca cagcctcatt tttgcgtaaa
    18841 aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca
    18901 cgatatacaa gttacctgtt tttccctgat ttttgttgat tgatattgat tgtctaatcc
    18961 ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag
    19021 gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag
    19081 tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt
    19141 aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt
    19201 tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc
    19261 atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg
    19321 atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt
    19381 atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat
    19441 acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt
    19501 atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac
    19561 ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc
    19621 gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt
    19681 ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta
    19741 caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg
    19801 aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt
    19861 ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg
    19921 gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca
    19981 gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa
    20041 gagcatcttg atgaattcca catttatacg ttctacagat attttaccta atagctcgtg
    20101 gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa
    20161 acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac
    20221 agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc
    20281 atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa
    20341 cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg
    20401 aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat
    20461 cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc
    20521 gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa
    20581 ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc
    20641 cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga
    20701 taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg
    20761 accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa
    20821 tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat
    20881 catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc
    20941 ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc
    21001 agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata
    21061 ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct ttttccctcc
    21121 aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa
    21181 tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg
    21241 aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata
    21301 ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa
    21361 gggggagtct cagggatcac cttgatctta agagctttat ttggtattga tccagcttat
    21421 acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt
    21481 tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa
    21541 agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt
    21601 ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt
    21661 gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg
    21721 gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg
    21781 tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga
    21841 tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg
    21901 atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat
    21961 tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc
    22021 gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg
    22081 caaggcttta cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga
    22141 caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta
    22201 tactattttt aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa
    22261 aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc
    22321 tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg
    22381 aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa
    22441 tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca
    22501 ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc
    22561 cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact
    22621 gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat
    22681 cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac
    22741 atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct
    22801 cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt
    22861 aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag
    22921 gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt
    22981 acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca
    23041 taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca
    23101 tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct
    23161 aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc
    23221 gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact
    23281 gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac
    23341 gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt
    23401 tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct
    23461 ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat
    23521 tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat
    23581 gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc
    23641 gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca
    23701 ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga
    23761 tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta
    23821 tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg
    23881 atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg
    23941 cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt
    24001 gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt
    24061 tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc
    24121 tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt
    24181 tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc
    24241 cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa
    24301 atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt
    24361 catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt
    24421 accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat
    24481 cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg
    24541 caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt
    24601 tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat
    24661 ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg
    24721 gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt
    24781 ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga
    24841 ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt
    24901 gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt
    24961 gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc
    25021 aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc
    25081 atcttctact tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc
    25141 attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa
    25201 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
    25261 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
    25321 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
    25381 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
    25441 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
    25501 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
    25561 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
    25621 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
    25681 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
    25741 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
    25801 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
    25861 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
    25921 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
    25981 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
    26041 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
    26101 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
    26161 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
    26221 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
    26281 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
    26341 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
    26401 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
    26461 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
    26521 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
    26581 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
    26641 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
    26701 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
    26761 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
    26821 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
    26881 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
    26941 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
    27001 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
    27061 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
    27121 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
    27181 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
    27241 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
    27301 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
    27361 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
    27421 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
    27481 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
    27541 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
    27601 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
    27661 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
    27721 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
    27781 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
    27841 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
    27901 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
    27961 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
    28021 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
    28081 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
    28141 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
    28201 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
    28261 aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt ctttatcttt
    28321 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
    28381 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
    28441 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
    28501 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
    28561 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
    28621 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
    28681 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
    28741 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
    28801 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
    28861 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
    28921 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
    28981 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
    29041 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
    29101 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
    29161 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
    29221 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
    29281 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
    29341 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
    29401 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
    29461 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
    29521 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
    29581 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
    29641 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
    29701 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
    29761 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
    29821 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
    29881 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
    29941 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
    30001 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
    30061 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
    30121 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
    30181 aaatcgttcc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
    30241 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
    30301 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
    30361 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
    30421 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
    30481 ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
    30541 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
    30601 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
    30661 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
    30721 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
    30781 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
    30841 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
    30901 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
    30961 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
    31021 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
    31081 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
    31141 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
    31201 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
    31261 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
    31321 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
    31381 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
    31441 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
    31501 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
    31561 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
    31621 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
    31681 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
    31741 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
    31801 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
    31861 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
    31921 aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt gttttttgcc
    31981 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
    32041 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
    32101 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
    32161 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
    32221 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
    32281 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
    32341 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
    32401 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
    32461 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
    32521 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
    32581 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
    32641 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
    32701 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
    32761 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
    32821 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
    32881 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
    32941 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
    33001 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
    33061 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
    33121 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
    33181 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
    33241 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
    33301 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
    33361 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
    33421 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
    33481 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
    33541 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
    33601 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
    33661 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
    33721 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
    33781 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
    33841 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
    33901 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
    33961 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
    34021 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
    34081 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
    34141 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
    34201 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
    34261 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
    34321 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
    34381 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
    34441 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
    34501 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
    34561 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
    34621 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
    34681 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
    34741 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
    34801 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
    34861 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
    34921 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
    34981 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
    35041 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
    35101 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
    35161 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    35221 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    35281 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    35341 agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg atagccgttt
    35401 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    35461 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    35521 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    35581 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    35641 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    35701 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    35761 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    35821 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    35881 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    35941 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    36001 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    36061 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    36121 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    36181 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    36241 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    36301 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    36361 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    36421 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    36481 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    36541 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    36601 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    36661 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    36721 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    36781 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    36841 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    36901 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    36961 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    37021 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    37081 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    37141 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    37201 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    37261 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    37321 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    37381 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    37441 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    37501 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    37561 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    37621 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    37681 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    37741 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    37801 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    37861 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    37921 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    37981 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    38041 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    38101 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    38161 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    38221 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    38281 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    38341 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    38401 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    38461 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    38521 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    38581 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    38641 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    38701 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    38761 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    38821 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    38881 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    38941 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    39001 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    39061 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    39121 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    39181 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    39241 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    39301 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    39361 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    39421 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    39481 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    39541 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    39601 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    39661 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    39721 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    39781 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    39841 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    39901 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    39961 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    40021 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    40081 gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    40141 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    40201 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    40261 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    40321 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    40381 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    40441 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    40501 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    40561 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    40621 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    40681 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    40741 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    40801 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    40861 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    40921 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    40981 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    41041 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    41101 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    41161 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    41221 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    41281 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    41341 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    41401 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    41461 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    41521 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    41581 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    41641 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    41701 tagacactca gacggactgg tttctttttt ctttttcata gcattcctga cagattccat
    41761 ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca
    41821 cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat
    41881 gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa
    41941 aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag
    42001 caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat
    42061 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
    42121 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
    42181 tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta
    42241 attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt
    42301 gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa
    42361 tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga
    42421 tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg
    42481 tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc
    42541 tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg
    42601 ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa
    42661 tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt
    42721 gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc
    42781 aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg
    42841 caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc
    42901 taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg
    42961 aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc
    43021 ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg
    43081 gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa
    43141 atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc
    43201 ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag
    43261 tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc
    43321 acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt
    43381 acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc
    43441 agattttgtt gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt
    43501 ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt
    43561 gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc
    43621 tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc
    43681 ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc
    43741 ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt
    43801 ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc
    43861 attcaactga gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt
    43921 gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt
    43981 ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat
    44041 caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa
    44101 cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac
    44161 tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata
    44221 tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat
    44281 catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg
    44341 aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg
    44401 atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag
    44461 ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt
    44521 tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc
    44581 aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt
    44641 gcttacagat tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt
    44701 tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc
    44761 cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc
    44821 ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc
    44881 ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag
    44941 cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag
    45001 tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg
    45061 ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt
    45121 tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc
    45181 gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa
    45241 ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga
    45301 tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc
    45361 ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga
    45421 tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta
    45481 ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa
    45541 aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc
    45601 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
    45661 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
    45721 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
    45781 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
    45841 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
    45901 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
    45961 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
    46021 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
    46081 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
    46141 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
    46201 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
    46261 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
    46321 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
    46381 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
    46441 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
    46501 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
    46561 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
    46621 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
    46681 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
    46741 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
    46801 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
    46861 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
    46921 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
    46981 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
    47041 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
    47101 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
    47161 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
    47221 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
    47281 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
    47341 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
    47401 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
    47461 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
    47521 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
    47581 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
    47641 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
    47701 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
    47761 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
    47821 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
    47881 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
    47941 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
    48001 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
    48061 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
    48121 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
    48181 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
    48241 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
    48301 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
    48361 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
    48421 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
    48481 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
    48541 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
    48601 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
    48661 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
    48721 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
    48781 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
    48841 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
    48901 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
    48961 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
    49021 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
    49081 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
    49141 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
    49201 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
    49261 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
    49321 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
    49381 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
    49441 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
    49501 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
    49561 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
    49621 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
    49681 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
    49741 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
    49801 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
    49861 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
    49921 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
    49981 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
    50041 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
    50101 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
    50161 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
    50221 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
    50281 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
    50341 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
    50401 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
    50461 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
    50521 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
    50581 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
    50641 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
    50701 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
    50761 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
    50821 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
    50881 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
    50941 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
    51001 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
    51061 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
    51121 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
    51181 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
    51241 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
    51301 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
    51361 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
    51421 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
    51481 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
    51541 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
    51601 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
    51661 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
    51721 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
    51781 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
    51841 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
    51901 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
    51961 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
    52021 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
    52081 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
    52141 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgta ctccttcttt
    52201 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
    52261 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
    52321 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
    52381 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
    52441 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
    52501 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
    52561 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
    52621 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
    52681 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
    52741 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctca taaaaacaac
    52801 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
    52861 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
    52921 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
    52981 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
    53041 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
    53101 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
    53161 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
    53221 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
    53281 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
    53341 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
    53401 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
    53461 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
    53521 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
    53581 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
    53641 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
    53701 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
    53761 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
    53821 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
    53881 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
    53941 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
    54001 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
    54061 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
    54121 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
    54181 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
    54241 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
    54301 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
    54361 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
    54421 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
    54481 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
    54541 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
    54601 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
    54661 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
    54721 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
    54781 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
    54841 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
    54901 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
    54961 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
    55021 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
    55081 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
    55141 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
    55201 cgttattggg gtatacaatc tgccggattt ccctcatcgg cgtcaccatt ttatttcctt
    55261 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
    55321 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
    55381 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
    55441 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
    55501 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
    55561 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    55621 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    55681 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    55741 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    55801 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    55861 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    55921 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    55981 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    56041 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    56101 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    56161 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    56221 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    56281 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    56341 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    56401 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    56461 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    56521 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    56581 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    56641 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    56701 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    56761 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    56821 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    56881 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    56941 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    57001 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    57061 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    57121 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    57181 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    57241 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    57301 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    57361 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    57421 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    57481 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    57541 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    57601 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    57661 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    57721 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    57781 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    57841 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    57901 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    57961 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    58021 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    58081 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    58141 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    58201 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    58261 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    58321 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    58381 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    58441 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    58501 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagag
//
LOCUS       DAZKAY010000008        49406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000008 DAZKAY010000000
VERSION     DAZKAY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.008"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..2709
                     /gene="adhE"
                     /locus_tag="ACG8WP_000429"
                     /gene_synonym="adhC"
     CDS             112..2709
                     /gene="adhE"
                     /locus_tag="ACG8WP_000429"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9026768.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2938..4044
                     /locus_tag="ACG8WP_000430"
     CDS             2938..4044
                     /locus_tag="ACG8WP_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9026769.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4061..5089
                     /locus_tag="ACG8WP_000431"
     CDS             4061..5089
                     /locus_tag="ACG8WP_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9026770.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5095..5574
                     /locus_tag="ACG8WP_000432"
     CDS             5095..5574
                     /locus_tag="ACG8WP_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9026771.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5590..6885
                     /locus_tag="ACG8WP_000433"
     CDS             5590..6885
                     /locus_tag="ACG8WP_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9026772.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6896..7720
                     /locus_tag="ACG8WP_000434"
     CDS             6896..7720
                     /locus_tag="ACG8WP_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9026773.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7885..8784
                     /locus_tag="ACG8WP_000435"
     CDS             7885..8784
                     /locus_tag="ACG8WP_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026774.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8910..9779)
                     /locus_tag="ACG8WP_000436"
     CDS             complement(8910..9779)
                     /locus_tag="ACG8WP_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9026775.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9932..11392
                     /gene="rhaB"
                     /locus_tag="ACG8WP_000437"
     CDS             9932..11392
                     /gene="rhaB"
                     /locus_tag="ACG8WP_000437"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9026776.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11389..12645
                     /gene="rhaA"
                     /locus_tag="ACG8WP_000438"
     CDS             11389..12645
                     /gene="rhaA"
                     /locus_tag="ACG8WP_000438"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9026777.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12659..13504
                     /gene="rhaD"
                     /locus_tag="ACG8WP_000439"
     CDS             12659..13504
                     /gene="rhaD"
                     /locus_tag="ACG8WP_000439"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9026778.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13506..13817
                     /gene="rhaM"
                     /locus_tag="ACG8WP_000440"
     CDS             13506..13817
                     /gene="rhaM"
                     /locus_tag="ACG8WP_000440"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9026779.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13899..15050
                     /gene="fucO"
                     /locus_tag="ACG8WP_000441"
     CDS             13899..15050
                     /gene="fucO"
                     /locus_tag="ACG8WP_000441"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9026780.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15177..15935
                     /locus_tag="ACG8WP_000442"
     CDS             15177..15935
                     /locus_tag="ACG8WP_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9026781.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16035..16679
                     /locus_tag="ACG8WP_000443"
     CDS             16035..16679
                     /locus_tag="ACG8WP_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9026782.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16686..17693
                     /locus_tag="ACG8WP_000444"
     CDS             16686..17693
                     /locus_tag="ACG8WP_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9026783.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17709..18551
                     /gene="kduI"
                     /locus_tag="ACG8WP_000445"
     CDS             17709..18551
                     /gene="kduI"
                     /locus_tag="ACG8WP_000445"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9026784.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18571..19374
                     /locus_tag="ACG8WP_000446"
     CDS             18571..19374
                     /locus_tag="ACG8WP_000446"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9026785.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19623..20687)
                     /gene="fni"
                     /locus_tag="ACG8WP_000447"
     CDS             complement(19623..20687)
                     /gene="fni"
                     /locus_tag="ACG8WP_000447"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9026786.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20684..21769)
                     /locus_tag="ACG8WP_000448"
     CDS             complement(20684..21769)
                     /locus_tag="ACG8WP_000448"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9026787.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21782..22759)
                     /gene="mvaD"
                     /locus_tag="ACG8WP_000449"
     CDS             complement(21782..22759)
                     /gene="mvaD"
                     /locus_tag="ACG8WP_000449"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9026788.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22752..23696)
                     /gene="mvk"
                     /locus_tag="ACG8WP_000450"
     CDS             complement(22752..23696)
                     /gene="mvk"
                     /locus_tag="ACG8WP_000450"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9026789.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24012..24671
                     /locus_tag="ACG8WP_000451"
     CDS             24012..24671
                     /locus_tag="ACG8WP_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9026790.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24756..25661
                     /locus_tag="ACG8WP_000452"
     CDS             24756..25661
                     /locus_tag="ACG8WP_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9026791.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GRGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25663..26295)
                     /locus_tag="ACG8WP_000453"
     CDS             complement(25663..26295)
                     /locus_tag="ACG8WP_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9026792.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26446..26571
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26615..27139
                     /gene="infC"
                     /locus_tag="ACG8WP_000454"
     CDS             26615..27139
                     /gene="infC"
                     /locus_tag="ACG8WP_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9026793.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27211..27411
                     /gene="rpmI"
                     /locus_tag="ACG8WP_000455"
     CDS             27211..27411
                     /gene="rpmI"
                     /locus_tag="ACG8WP_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9026794.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27464..27823
                     /gene="rplT"
                     /locus_tag="ACG8WP_000456"
     CDS             27464..27823
                     /gene="rplT"
                     /locus_tag="ACG8WP_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9026795.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28075..29412
                     /locus_tag="ACG8WP_000457"
     CDS             28075..29412
                     /locus_tag="ACG8WP_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9026796.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29460..31589
                     /locus_tag="ACG8WP_000458"
     CDS             29460..31589
                     /locus_tag="ACG8WP_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9026797.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31564..32253
                     /locus_tag="ACG8WP_000459"
     CDS             31564..32253
                     /locus_tag="ACG8WP_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026798.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32661..33274)
                     /locus_tag="ACG8WP_000460"
                     /pseudo
     CDS             complement(32661..33274)
                     /locus_tag="ACG8WP_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33410..33604)
                     /locus_tag="ACG8WP_000461"
     CDS             complement(33410..33604)
                     /locus_tag="ACG8WP_000461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026799.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33654..34094)
                     /locus_tag="ACG8WP_000462"
     CDS             complement(33654..34094)
                     /locus_tag="ACG8WP_000462"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9026800.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34098..34943)
                     /locus_tag="ACG8WP_000463"
     CDS             complement(34098..34943)
                     /locus_tag="ACG8WP_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9026801.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35092..36510
                     /gene="pepV"
                     /locus_tag="ACG8WP_000464"
     CDS             35092..36510
                     /gene="pepV"
                     /locus_tag="ACG8WP_000464"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9026802.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36567..37514)
                     /locus_tag="ACG8WP_000465"
     CDS             complement(36567..37514)
                     /locus_tag="ACG8WP_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9026803.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37737..38087)
                     /locus_tag="ACG8WP_000466"
     CDS             complement(37737..38087)
                     /locus_tag="ACG8WP_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9026804.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38287..39366
                     /gene="pepA"
                     /locus_tag="ACG8WP_000467"
     CDS             38287..39366
                     /gene="pepA"
                     /locus_tag="ACG8WP_000467"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9026805.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39510..39830
                     /locus_tag="ACG8WP_000468"
     CDS             39510..39830
                     /locus_tag="ACG8WP_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9026806.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39852..40316
                     /locus_tag="ACG8WP_000469"
     CDS             39852..40316
                     /locus_tag="ACG8WP_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9026807.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40521..41126
                     /gene="ytpR"
                     /locus_tag="ACG8WP_000470"
     CDS             40521..41126
                     /gene="ytpR"
                     /locus_tag="ACG8WP_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9026808.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41165..42454)
                     /locus_tag="ACG8WP_000471"
     CDS             complement(41165..42454)
                     /locus_tag="ACG8WP_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9026809.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42882..43361
                     /gene="rlmH"
                     /locus_tag="ACG8WP_000472"
     CDS             42882..43361
                     /gene="rlmH"
                     /locus_tag="ACG8WP_000472"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9026810.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43446..43955
                     /locus_tag="ACG8WP_000473"
     CDS             43446..43955
                     /locus_tag="ACG8WP_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9026811.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44055..44705
                     /locus_tag="ACG8WP_000474"
     CDS             44055..44705
                     /locus_tag="ACG8WP_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026812.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            45156..46094
                     /locus_tag="ACG8WP_000475"
     CDS             45156..46094
                     /locus_tag="ACG8WP_000475"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9026813.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            46107..47159
                     /locus_tag="ACG8WP_000476"
     CDS             46107..47159
                     /locus_tag="ACG8WP_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9026814.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            47425..48060
                     /locus_tag="ACG8WP_000477"
     CDS             47425..48060
                     /locus_tag="ACG8WP_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9026815.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            48251..49042
                     /gene="msrA"
                     /locus_tag="ACG8WP_000478"
     CDS             48251..49042
                     /gene="msrA"
                     /locus_tag="ACG8WP_000478"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9026816.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        15684 a         8810 c        10569 g        14343 t
ORIGIN      
        1 aaaataactg tgaaaaaaat cacaaaaaag tgtttacata gaaaaataaa tattgtatga
       61 tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat gatggctaaa
      121 gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga tgatttggca
      181 gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa agtagaccat
      241 atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc gaagatggct
      301 gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc
      361 gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc
      421 caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt aactccaaca
      481 acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca
      541 atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc acgagtggta
      601 cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat cgaacatcct
      661 tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt cttagcgaca
      721 ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt aggtgtaggt
      781 ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat
      841 ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca agctgtgatc
      901 gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca agtttatatc
      961 gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg
     1021 gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc aggaatcagt
     1081 gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc agattatccg
     1141 ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa cacggaccat
     1201 gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac agcagttatc
     1261 cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt
     1321 ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga aatgattccg
     1381 tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa cgtctctgct
     1441 gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca atggttcaaa
     1501 ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa aatggaaaac
     1561 gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta ctgcgataca
     1621 gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt
     1681 gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc agacttcaag
     1741 ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg
     1801 atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt cttggatatc
     1861 cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg tatcccgaca
     1921 acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag tgaaacacat
     1981 gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa
     2041 ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt gttaacacat
     2101 gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt gagcctacaa
     2161 gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga tatggaatca
     2221 cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa cgcattccta
     2281 ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc tcatggacgg
     2341 acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc acaaaaacat
     2401 gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga tatcgcaaaa
     2461 ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc aacggctgtt
     2521 tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg agtcacacaa
     2581 gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga ccaatgtacg
     2641 acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat tgatgcttac
     2701 aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa aagaacttgg
     2761 aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt tacttttgtt
     2821 atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt tatagtggac
     2881 ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg
     2941 attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat tgacctgctc
     3001 gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga ctttgatgga
     3061 ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt cggtctatat
     3121 ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa
     3181 tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg
     3241 ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt gccttattta
     3301 gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga cgggttacag
     3361 catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt gatgatgaca
     3421 gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt agaagaagct
     3481 cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg attgtggtat
     3541 cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc tcgtggaaac
     3601 tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat
     3661 gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat
     3721 cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct
     3781 tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca
     3841 caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa
     3901 aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat
     3961 cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca
     4021 gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct
     4081 gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc
     4141 tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc gctttgctta
     4201 tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga
     4261 aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg
     4321 tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc
     4381 gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat tcaatgatga
     4441 agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac
     4501 caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat
     4561 ggtagacggt ccgatccata cgccggaaga tttggaggga aagaagatcc gtgtgatgca
     4621 aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac ctatgggaag
     4681 tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt
     4741 tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg atgagcatac
     4801 acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa ctgatgagca
     4861 ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg aagcttttgc
     4921 aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc aattcaatga
     4981 tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat tcaaaaacga
     5041 tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa
     5101 atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg gatggtcgct
     5161 gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac ggtcacggaa
     5221 gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta tgttgtggga
     5281 caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa aaaaagtaaa
     5341 attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat catggtgtat
     5401 ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt aagtattcct
     5461 atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat ctatagcctg
     5521 atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg
     5581 aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt tgtgttgctt
     5641 gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac tttattgtta
     5701 gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg aagtatcaat
     5761 agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat gaataatgga
     5821 gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt gccgggggct
     5881 ttggctcata cgaatgtgtt agggaatgct ttatttggtt ccatctctag ctcggccatt
     5941 gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga aggatatgac
     6001 cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt cattcctcca
     6061 agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc tgctttgttt
     6121 atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt agcattcatc
     6181 gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca tgctggaaaa
     6241 ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg tggtatcttg
     6301 acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc cctgttgatt
     6361 gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt gtttcaatca
     6421 gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat gtctttcgcc
     6481 atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt aacggataat
     6541 aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat gtttatggat
     6601 gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac aagtgtaggg
     6661 gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg ctccatcacg
     6721 ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca
     6781 atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt tttgattact
     6841 tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa tagttatgtt
     6901 gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag atcttgcaga
     6961 aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt cgtttcctga
     7021 aacaccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca aacgagtttt
     7081 agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga ttcatccaga
     7141 tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct atgcttcttc
     7201 ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa tccaatatac
     7261 ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt
     7321 aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga atcatccggt
     7381 gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact atctaggaat
     7441 cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag tccaattggt
     7501 agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga aagattttcg
     7561 tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa
     7621 aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa
     7681 agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag acgttggtta
     7741 actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc ttttttataa
     7801 gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa attgtgcata
     7861 agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga acaagtcaat
     7921 acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa
     7981 acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg agaagcgact
     8041 ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct catccattcc
     8101 aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt
     8161 gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc ggcttgtttt
     8221 caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa
     8281 aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga cataaaatat
     8341 gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa agaagagagt
     8401 gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt acaatatatc
     8461 tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc
     8521 aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca tcagtatgta
     8581 atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag ctctagtgct
     8641 gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt caagagagag
     8701 ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt ggttcattat
     8761 gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag tagctgctgt
     8821 tgtttacata gaagcagaaa ctgcggttat tttattttta tctatatatt tcaaaatatt
     8881 ttgaaaaaat gacgtacata ttatatgaat cagctgaatt tacgaaagtc tctcggcgat
     8941 agtcctgttt ttgctttgaa caggcggcta aagtaagcaa aattctcaat ccctatttct
     9001 tcggaaatca ttcgaaccgt aaattctgta taccgaagaa ggtctttagc gtaagaaagt
     9061 tttttgctga tcaaataatc gattggtgga gaaccgataa atttggaaaa atctttgctc
     9121 agctgatatt tattcaataa gaattgttgt tctaattgat caagtgtaat cgtttctttg
     9181 aaatgttcgt ctaagtattg cttcagtttg taaacgtagg cgggaatatc ctcttcttcg
     9241 aaatctagct gatacttttg caaaatcagt tcattcagca actcgtggat agctacagag
     9301 gactggaaat cggtacgggc attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa
     9361 tggtggatct tgttttctga tggatcccct gttgaatgga aggtgttatg gccgtctttt
     9421 gtgaactcct gataaaatga ttggacgttc tgaccattca aatggatcca atccatttcc
     9481 catggttctt cgctgatcgt ttgataacgc tgatattctt ggcagtcaat aaaaaataag
     9541 tctccagctt ttagctgttg ctgattatca ttatatgtaa gcgaaccttt gccagacaaa
     9601 gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg gattggtgct
     9661 ttgaagtaac cgatttcttg gatataaaaa aataaattcc tgcttctttg tgtaggcgtc
     9721 aagatcactc tttttgagtc ttccgaccat gagtggttgg ctttttccca gtaatccatg
     9781 taaacactcc caacttgtca agataatgca attatatacc aaaatagtgt gaagcaaaat
     9841 ataagattct gtattatgat agcattgtta ataaatgata acgctttatt aataaagtgc
     9901 taatcaacaa taaagtaaaa gaggtggaga aatgaatcca acatttgttg ccatcgatat
     9961 cggtgcatca agcggacgac taatgaaaag tacgagggat gcaaaaggtt ctttgatctt
    10021 ggaagaagta caccggttca aaaacggctt tcggaacaaa gacggtcacg atcgctggaa
    10081 gatcgattat cttatcaaag agattttgat tggatttgaa aaattaaagc agacagggat
    10141 tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat tactgtctga ttgatgaaca
    10201 aggggatcgg ataaatgatc cgattgctta tcgggacaag cgtaccaatg gagcagtaga
    10261 acagttttct gaaatctatt ctctagataa gttgtatgag cagacaggga ttcagatcca
    10321 gcctttcaat accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca
    10381 taaattgtta ctgatacctg attatttagg gtatgtcttt actggtaaca tggtgacgga
    10441 gaaaactaat gcatctacta tgcagctgct gaatgcaggg acaaaagaat gggaaataga
    10501 acttcttgaa aaaatcggtg tctcgaagga attatttcca ccattgacag agccgggaac
    10561 gatactagga gatctgcaaa aagaacaatt ttcaaaatat gatttgccag atgtgacctt
    10621 tatcacagta gcaagtcatg atacggcgtc agctgtatta ggaacgccag gtcaaggaga
    10681 acgttggggt tatatcagca gtgggacatg gtcgctttta gggattgaaa caaagatcgc
    10741 caatgtttct cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg cccataacac
    10801 ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc caggaagttg cccgaatgca
    10861 agagtacgag tacagttatg cggaattggc agagcttgct gcaaaagaaa aaccgctgca
    10921 gcaagtgatg gaaatcaatg accctcgatt tttgaatccc ccaaatatga tagaagagat
    10981 tcagcaatac tgttttgaaa cagagcagcc agtccctgtc tcatcgggag ctatcgctcg
    11041 ttgtgtgtat gataatcttg ctctttgtta cgcacaagag ctgatcaaag tagaacactt
    11101 aactggaacg aaattggaaa agctgcacat cgtagggggc ggatcgaata atcgtctact
    11161 gaatcaattg acagcagatt atgccggaat cattgtagaa gcaggaccta gtgaagccac
    11221 agcaatcggg aatgttgcca tgcaaatgct cagtacaggt cagttttcga atatagaaca
    11281 agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact ttttacccag atgaaaataa
    11341 gcaaaacacg atggaatggt acgtgaataa aatcaaagga gtaatcgtat gaacacgatt
    11401 gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct
    11461 gtactagaaa agatacagga catcaaaatt tctatgcatt gctggcaagg agatgatgtc
    11521 aaaggtttct tgacaccgga tggcgaattg accggtggaa tcatggcgac agggaatttt
    11581 ccaggagccg cacgaacacc tgaagagctt agacaagatc tggagaaggc ttattcgttg
    11641 ataccgggca aacacaaatt gaaccttcat gctatctatt tggatacaga agaatctgtc
    11701 gatctaaatg aaattgagcc gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa
    11761 gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc caatgatgaa agacggcttt
    11821 acattggctc atccagataa agaagttcgt gaatactgga tcgaacacgg gaaaagaagc
    11881 cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa caagtatcaa taacttttgg
    11941 gtacctgatg ggatgaaaga taatccgatt gatcgtttta ccccacggga acgtttaatg
    12001 gaatcactcg atgaaatttt tacagaagag ttagatgaac ggtatgtaca agatgcagta
    12061 gaaagcaaac tatttggttt aggcgctgaa gcttatacag tcggttccca tgaattttat
    12121 atgggttacg ggttgactcg tgacaaactt gtttgtctag atgctggaca ttttcatcca
    12181 acagaagtga tttccaataa attatcttca ttagctttat tcagcaaagg aatcatgttg
    12241 catgtttccc gtccagtgcg ttgggattca gaccatgtgg ttttgatgga tgatgaattg
    12301 caggaaatcg ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg
    12361 gatttctttg atgcaacgat caaccgtatc gctgcttggg tcatcggtac gagaaataca
    12421 caaaaagcac tgctgaaagc tatgctggaa ccagttgagc gattgaaaga aatggagctg
    12481 gcttttgact ttacttcaag aatggcttat acggaagaat tgaaagattt cccgtatgcc
    12541 gatgtatgga attatttctg ttacaaaaat caagttccag ttggtttgga ttggctggaa
    12601 gaagtccagg aatatcaaaa agacgtgttg gaattaagaa aataggagtg tggctatatt
    12661 gaaaaaaata gatattttac aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa
    12721 tctttatcgc ttgggatggg atgaaagaaa tggcgggaat atttcttatt tactaaaaga
    12781 agaagagatc acaccgttct tggatcctga aaaagtgatc cgccgcatct cgatgatttt
    12841 tgatgccagc cgtttagcag gatgctattt tattgtaact ggttcaggaa gatattttaa
    12901 aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt cgagttgctg aagatggcaa
    12961 aacactggaa cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg agttgccaag
    13021 tcatttcatg agtcatatcg cccgtttaga agtagaccca gataatcgga tcgttatgca
    13081 ttgccatgct agtcatctat tagcgatgag ttttacgcat gaattagacg aacgttcctt
    13141 tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt gtttttccag aaggaatttc
    13201 catcattccg tggatggttc cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat
    13261 gaaagaaact cgattggttt tatggccaca acatggaatc tacgggtcag gaaaagacat
    13321 ggatgaagtc tttggtttga tcgagacagc agaaaaggca gcagaagtct atacgtacgt
    13381 caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa aacctgtggc atttagcaga
    13441 tgcgtttggt gtcactccaa aagcaggtta tttggaggaa gttcatacga aagcaggtgt
    13501 ctgacatggt gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa gaagaataca
    13561 aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa cacggggtca
    13621 tcgactattc gatctatctt gatccgagta caagtttgtt atttggttac ttagtgatcg
    13681 aagatgaaaa gttatgggaa aaaatggcaa atacatctat caatcaaaaa tggtggaagt
    13741 acatggaaga tatcatggag acaaatccag atgcctctcc agtcagttgg gaattgatgc
    13801 cagtctttga actgtagcaa caagcaactg ggcaaccggt tgcttggggt tctttcgttt
    13861 ttattgtgaa aaaaaataca aattaggagg aatagaaaat gagtaatcga atgattttaa
    13921 atgaaacatc gtattttgga aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc
    13981 gaggattcgg caaagcagct gtcattactg acaaaggatt gatcgagcac ggtatagcaa
    14041 caaaagtaac agaacttcta gataaagcag caatcgctta tgctttatac gacgggatcg
    14101 tacctaatcc aacgatccaa aatgtaaaag atggagtggc ttttgttaaa gaggcagagg
    14161 cagactgttt gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa gccattggga
    14221 ttattttgac gaatccagag ttttcagacg tcattagttt agaaggtgtt gctgatacga
    14281 aaaatccatg tttacctata ttagctatac caacgacttc tggaacagca gcagaagtaa
    14341 cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc
    14401 atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat
    14461 tggctgcatc aacaggtatg gatgctatga cgcatgctat tgaagggttt atcacaaaag
    14521 gcgcttggga aatgactgat atgctgcatt taaaagcaat cgaaatcatc ggccattctt
    14581 tggaagtttc tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta ggacagtata
    14641 ttgcgggaat gggcttctca aatgtcggtt taggactggt acacgggatg gctcacccgt
    14701 taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc agcacttttg ccaactgtca
    14761 tgaaatataa caaagaatat actggagaaa agtaccgtga gatcgctctt gttctaggga
    14821 ttaaaggagc agctgaaatg tctttagaag atgtaagaga agcggcttgt ggtgagatcg
    14881 atcggttgag caaagcggtt ggaatcccag aaaccatttc tgaattaggt gtaaaagaag
    14941 cagatattcc agcaattgca gaagatgctt tgcgagatgt atgcacacca ggaaatccaa
    15001 gagaaacgac agtagaagaa atcattgctt tatatcaaag tttgatgtaa aaacgaagcg
    15061 gatcttcaac attaaattgc ctgtaaagag aaatggtagt ttatatctgg atatctcttt
    15121 taaattgatg gtatttccgc tataattttt cttataaaga aataggagaa tattatatgg
    15181 aaaataaaaa gccttatgga actgtattga tcaaagcctc taaaatacta gactatttag
    15241 cggaaaacca agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg acctcttcta
    15301 cagtcttgaa aatactagat actttgttga tgatcggcta cgtaaataaa aattcagaga
    15361 aaaactatcg tttaggtgca aagcttattc gctacgcgaa taaaaacatt gaacaaatcg
    15421 atttggtaga gttgacttta ccgtttttag aacagctgca gcaaaagatc gatgagacta
    15481 tccacttagg ggtattggac aacaatgaga tcttgtacgt gaacaaacta gatccaaaac
    15541 atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg accattatac aattctgcga
    15601 tgggcaaagc cgtattagca gaattcagtg aagagcagta cactgattat cttgaaaagc
    15661 atccgttgat cccttacaca gaatatacga tcaccaatgc attacggttg aaaaaggaaa
    15721 tccaaacggt aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa cgtgatattt
    15781 tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt agtgtaagta
    15841 tgccgaaata tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc ctcaagcaaa
    15901 caaaaagcga aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg caatttttct
    15961 tttctgccgt ataatatggg tatagatttc caagttataa aataaaattt ctaaatagga
    16021 aataggtgga gaagatgaaa aaaattgata ttttatctcg attgaaaaac gccggagtga
    16081 ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa tgcttgccac gcaatcatca
    16141 aaggtggtct aactggtatt gagttgactt ttacagtccc tcaagcagat caagtaatca
    16201 aagaactttt gtcattctac aaagaccaac cagaaatagt gattggcgca ggcacagtat
    16261 tagacgctgt cacagctcgc ttggctatct tagctggcgc agagtatatt gttagccctt
    16321 cctttgatca agaaacggca gagatgtgca atctgtatca aattccttac ttaccaggat
    16381 gtatgactat taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc gtcaaactat
    16441 ttcctggaag cgcttatgga ccaagtatca tttctgcctt caaggcgccg atgcctcaag
    16501 tcaatatcat gccaactggc ggtgtgagtc tggacaatat gaaagagtgg ttcgatgctg
    16561 gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc cgcagctaca ggagattttg
    16621 acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc aagaggtagc
    16681 tgcttatggg aaaagtagtg acactcggag aaatcatgtt gcgattgtcc acagagcagg
    16741 gaacccgtat cgattcaagt gaacgattcg ctgcccatta tggcggaggg gaagcaaacg
    16801 tagcaatttc tttagctaat tacggacatg aagcgatttt tgctacaaaa gtacctgata
    16861 acgcattggg aaaagcagtc aaaaagcact tgcaatgtta tggagtagat acgaaacagg
    16921 tattatctgg tggatcaaga ctaggtactt attatatgga aactggtagt ggcgaacgcg
    16981 ccgcctcagt gatctatgat cgtgccggat caagttttgc tgaaatggat catttggaat
    17041 ggtcgctaga ggagttattc caaggaatag atatatttca tgtttcagga atcaccccag
    17101 ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat agaagctgca aaaaaagctg
    17161 gctgcctgat cagcttcgat atcaactatc gtggaaaatt atggacacag caagaagcca
    17221 gcgaagtaat ccatcagctt ttacctttag tagatatctg ctcagctgga aaaatggatg
    17281 ctctttattt gttaggtatc gagaaagcgc cagaagcaga agaacaacct ctaatttatt
    17341 attatcaaaa aatgcaggaa aagttcccga atattcaggt gttttattcg accaagcgta
    17401 ccgttcattc agcaagtgaa aatgaattga aggggacatt gtggatgaac catgaatatg
    17461 tggaatccca agtccataga atcaccccaa tcgtggatcg agtaggtggc ggagatgctt
    17521 ttgcaggtgg cgtgctccat gggattttat ccaatatgtc tccgcaagaa atcattgatt
    17581 ttgctacggc agcttctgcc ttgaagcata cgatccatgg ggactgcaac caatttagtc
    17641 aagaagaagt cgcacaattt ttagcatgcg gctctggaaa aatcattcga tagaaggagt
    17701 agaagaaaat gcaagaaatg gaaacacgtt atacacacag tccagaagat atccgtcatt
    17761 attcaacgga acaattgaga aaagaatttt tagtagaaaa ggtatttgaa ccagggaaaa
    17821 tcagtttgac ttatacgcac aacgatcgaa tgatctttgg tggagtgaca cctactgaaa
    17881 aagaattaga aatcacatta gacaaagaac taggtgttga ttacttctta gagcgtcgtg
    17941 aactaggtgt gatcaatatc ggtggaccag gattcatcga gatcgatggg caaaaagaag
    18001 aaatgaaaaa acaagacgga tactatatcg gaaaagaaac acgacatgtt gtattttctt
    18061 ctcaagatgc tgaaaaccca gctaaattct atattagttc tgttccagct catcataaat
    18121 atccaaatgt aaaaatcagt atcgaccaag taaaaccaat ggagactggt gaaggcctga
    18181 cactgaatga acgtaaaatc taccaataca tccatccaaa tgtatgcgaa agctgtcaat
    18241 tacaaatggg ttatacgatt ttagaaccag gaagcgcttg gaatacgatg ccttgtcata
    18301 cacatgaacg tcgtatggaa gcttatgtct actttgatta tgccaatgaa gatacacgtg
    18361 tgttccacat gatgggcaaa ccagatgaaa caaaacattt agttgtagac aatgaacaag
    18421 ccattatttc gccaagctgg tcgatccatt caggtgtagg tacaagcaat tactcattca
    18481 tctgggcaat gtgcggagaa aatatcacct atacagacat ggatatggtg caaatggatc
    18541 aattaaaata agcataaggg agagaaaatg atggaattta gtatggatat gtttcgttta
    18601 gatggaaaag tagcattagt caccggtgct gtctatggta ttggatttga gattgcacgc
    18661 tctttagcta cagcaggagc aaaaatcgta tttaataact tgaatcaaga atcagttgat
    18721 gaaggtatag cgcattataa agaagcaggc atcgatgcaa aaggttatgt ctgcgatgtg
    18781 accgatgaag aagcggttca agcgatggtt caacagatca aagaagatgt aggttcagtt
    18841 gatattctag tcaacaatgc agggatcatc aaacggacac cgatgatcga aatggatgcg
    18901 gcagacttcc gccaagtaat cgacgtggat ttaaatgccc cattcatcgt ttcaaaagca
    18961 gttattccgg atatgatcga aaaaggcggc ggtaaaatca tcaatatctg ctccatgatg
    19021 agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg attaaaaatg
    19081 ctgacgaaaa acatcgcttc tgaatacggt caatacaata tccaatgtaa tggaattgga
    19141 ccaggttata ttgctacacc gcaaactgct ccattacgcg aaacacaaga aaacggtgaa
    19201 cgtcatccat tcgatcaatt tatcgttgga cgtacgccag cagcacgctg gggcgagcca
    19261 atcgatttgg ctggaccatc tgtcttcttg gcttctagcg catctgattt cgttaacggc
    19321 catgtcttgt atgtggacgg aggcatactt gcctatatcg gaaaacaacc ttaaaagagc
    19381 tttagtacac tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa taccgaagga
    19441 cagctgatta aacatcattt attcgaaaaa agaggctggg acagaagcat ttaactccaa
    19501 gaaataagaa ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc
    19561 cgaaggagtt gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt
    19621 ctttactttt gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct
    19681 cttgcttcgc accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc
    19741 gcggtattcg ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc
    19801 atcagtataa tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa
    19861 gtacccacgg catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata
    19921 cctccggagg ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag
    19981 actgtcgatt gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt
    20041 tcgatctggg tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa
    20101 gcagctagat cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg
    20161 gggacgtcaa tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg
    20221 tctccctcag gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg
    20281 tgaaacaatc ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca
    20341 atgattttcc cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg
    20401 actttcaagg cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg
    20461 attgcaagat cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata
    20521 taaaatgggc tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact
    20581 gctatttgcg gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta
    20641 tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc
    20701 tttgatcgct atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca
    20761 ttaaaggaag aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg
    20821 caccagaaga ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag
    20881 caggcgtttc gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt
    20941 ttcgaatcat ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt
    21001 tgacacattc tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg
    21061 aacggtgaac ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca
    21121 ataaacgtaa atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg
    21181 caagtaactc actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg
    21241 ttgagaaagc gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat
    21301 cttgaactgc taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca
    21361 acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca
    21421 atccatattt ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg
    21481 ataaaagaat gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat
    21541 aatgaaaagg attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta
    21601 caggcatccc actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag
    21661 tgactgtcac gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg
    21721 catattctcc ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc
    21781 tttattcaat aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa
    21841 ataatttggt aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga
    21901 cattagggcc agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca
    21961 ccgcatccat cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta
    22021 aagtcgttcc atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat
    22081 cttttgtttt gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc
    22141 cttgataaaa actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt
    22201 ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag
    22261 ggactggctt agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac
    22321 cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat
    22381 catctaatcc aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg
    22441 cagaggaggc taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta
    22501 gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt
    22561 tttcgtcttg caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga
    22621 tgacttctgt ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa
    22681 ggatgagttc ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc
    22741 gtgctttgcc tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca
    22801 cccgcatctt caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca
    22861 atcatacagc caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt
    22921 gctgtttgga tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat
    22981 tgtgattcat ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt
    23041 gcttgcttcg tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt
    23101 tcaaagaggt gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca
    23161 gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta
    23221 aaataaatcg gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccgtgg
    23281 gcgatttttt ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct
    23341 aagtaatcat aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca
    23401 cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt
    23461 ttttttaagt gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa
    23521 tttcctgaat agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat
    23581 gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg
    23641 tgctcgccca tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc
    23701 taactccttc tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg
    23761 cgctgcttca cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt
    23821 actaaaacca tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt
    23881 cttaagaatt ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact
    23941 agatattggg ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga
    24001 gctggaggta aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt
    24061 taacagaaat gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata
    24121 atgaagtctt gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt
    24181 attttttgaa taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat
    24241 tggaccgttc gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag
    24301 caacagaaac agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc
    24361 cctatgctgc ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata
    24421 cgatgttgga gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa
    24481 gtctatacga ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca
    24541 aagaaagtcc aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag
    24601 caaaagaaga agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag
    24661 tagccatttg atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt
    24721 ctttatactg gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc
    24781 aagaccgtaa tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa
    24841 aagaagaata tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg
    24901 cgcgcgtaga gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc
    24961 agcaaaaagt cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg
    25021 gtttgtttac atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt
    25081 tggaacgaat gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt
    25141 ctgattcctt ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga
    25201 aattaagaga aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt
    25261 tggtttactt agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat
    25321 tggctattta tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg
    25381 aagcacgaca agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg
    25441 gcacttataa taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct
    25501 ggtcgctgat cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt
    25561 taccctttac ccattccgtt ttcggacggg gaatcgcctt gatcatcagt ttggtcttat
    25621 ctatttggat gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa
    25681 accaagggct tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga
    25741 atcttgagat aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga
    25801 agttcgaaac cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt
    25861 gacctcataa aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt
    25921 tgtactacta aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata
    25981 actttcggca aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact
    26041 gccaccaatc cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc
    26101 aaaatgacgt aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac
    26161 catatttttg gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat
    26221 ctcctcatct tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg
    26281 ttcgattttt ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat
    26341 cactgctaga aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa
    26401 aacattgaca aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag
    26461 cacccgcttc tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat
    26521 taggtgaagt acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt
    26581 aagcttttct ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt
    26641 taacgacggc attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg
    26701 agttaagaca aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt
    26761 agttgcccct ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt
    26821 cgaacaacaa aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga
    26881 agttcgctta agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg
    26941 taagttcttg gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat
    27001 tacccataaa gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat
    27061 tgcaacagtg gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc
    27121 gaaaaacgac agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat
    27181 aacgaaagat tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca
    27241 gcaaaacgtt tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt
    27301 caccgtttcc acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt
    27361 tctagtggcg acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa
    27421 ataacagaat ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg
    27481 tggaacagta actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg
    27541 ttcaaaacac actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc
    27601 atatcgcgat cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa
    27661 cgcagcagct cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc
    27721 tgaaatcgac atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt
    27781 tactgcattg gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag
    27841 aggaaagaag ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc
    27901 aaatgaaaca ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga
    27961 aaactgaaca tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt
    28021 ttgactgata tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt
    28081 gatattatta ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta
    28141 aaaggctaca acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg
    28201 ctagtcggaa acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg
    28261 ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc
    28321 ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt
    28381 cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt
    28441 gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt
    28501 attgtgttgc caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt
    28561 atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg
    28621 atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt
    28681 ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg
    28741 aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca
    28801 atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca
    28861 atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc
    28921 ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat
    28981 gttgcaccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat
    29041 gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg
    29101 gcaatcgtat ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc
    29161 ggttcaggct cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc
    29221 tttttgttcc cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa
    29281 tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa
    29341 tccatcccaa ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac
    29401 gggaacgcct agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa
    29461 tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa
    29521 tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg
    29581 tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg gaaaataata
    29641 tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga
    29701 ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt
    29761 ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag
    29821 agattgtcaa tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga
    29881 tttcttcttt agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag
    29941 ataatgggga accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa
    30001 cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa
    30061 tcaatgcatc tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac
    30121 gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa
    30181 tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca
    30241 tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa
    30301 caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg
    30361 gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga
    30421 tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg
    30481 gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt
    30541 tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag
    30601 aagtgactat tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt
    30661 tttcttatgg aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc
    30721 atacgacggt tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac
    30781 cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag
    30841 gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat
    30901 attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc
    30961 gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa
    31021 atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg
    31081 tagaaatcga tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga
    31141 aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg
    31201 atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat
    31261 gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat
    31321 ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga
    31381 cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac
    31441 cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac
    31501 aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg
    31561 tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag
    31621 atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt
    31681 cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca
    31741 tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct
    31801 gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat
    31861 gcagaagatg aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga
    31921 ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac
    31981 cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg
    32041 ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt
    32101 ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt
    32161 tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa
    32221 agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca
    32281 gtcctttttt tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata
    32341 ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc
    32401 aggctcacga cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag
    32461 ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc
    32521 agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca
    32581 aaaaaaacgg caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa
    32641 aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg
    32701 gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa
    32761 agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca
    32821 tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc
    32881 aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa
    32941 caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact
    33001 gcctgatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca gatcggatat
    33061 cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct tgattgcgca
    33121 gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct tctaacactt
    33181 tatcggagat tttacttcca ggtgctaact cggaataaat gatttgttta cgtatagatt
    33241 cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta tgcagatata
    33301 atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag tctgcaaaaa
    33361 aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat caatctggtc
    33421 gtacctcttg tatcggtttg acacgaccgc cgattctttt agcaaaagct tcggattctt
    33481 ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga cagccagttt
    33541 tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc ttttttcttt
    33601 tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct ttattaagaa
    33661 tttacatcat ccaaagaagc gtacgcttgg aataactgat ctaactccgg attgactttg
    33721 ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact actgccagta
    33781 gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa ccgtgtcccc
    33841 gtatactttt ggatagttgg atggatgctc actttttctc cgtaatggat catttcttta
    33901 tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat ttttttatag
    33961 ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg gatataattg
    34021 ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg cacataacct
    34081 gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa ataattcatc
    34141 catttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc ttttgccagc
    34201 tggtctccaa tgaaactatg aagataaata gctgctgcaa tcgtttggtc attttgagga
    34261 aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc gcccgttgcc
    34321 atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat ttttgtctca
    34381 tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt ttctttatcc
    34441 cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg aggtgtaagt
    34501 acagtttgtt cgggataggg tagcaaaaga tgattagcag caagtaaact taaagcagag
    34561 ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt gaaacgttcc
    34621 aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc ttgctttatt
    34681 acggaaagaa actggtcagt atccgtccaa actacaatca ttgcttctgg cagatgactg
    34741 tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc tccgctgtgt
    34801 acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc gccaatcaaa
    34861 acgatacgtc caaatgttcc tttatgtgaa atttttggac gaggtgtgat gactcttttc
    34921 actagttgtt cgtttatcag catctaaaaa cctcctttta ctcattatag cgtatatttt
    34981 ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat ttttttctgt
    35041 aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt tatgacaatt
    35101 gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt gaaaaatctt
    35161 ttacgtgtca acagcgagcg cgatgactca aaagtaacac cagatgcacc atttggacct
    35221 ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg atttaaagta
    35281 aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga aacgctaggg
    35341 atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga tccttatgaa
    35401 ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa aggtccaagt
    35461 atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact atcaaaaaaa
    35521 gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc ttattatttc
    35581 gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc aatcatcaat
    35641 ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc aggagattat
    35701 gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac agcaactgca
    35761 gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt ttaccaattc
    35821 atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt aaaaatcgaa
    35881 ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc aggttctttc
    35941 ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt tatccatgta
    36001 gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc ttatgaagat
    36061 gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga aaatggtaca
    36121 gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac agtagacgga
    36181 ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac tcgaggtgct
    36241 cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac attattgcaa
    36301 gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg cggaacttac
    36361 ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata tactgataca
    36421 atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac agcaatctat
    36481 gcagatgcca tctatcgttt ggcgaaataa ttaattaaaa agaggatcaa gaattgcaaa
    36541 ttatttttgc agtcttgatc ctttttttac tttctactgt ggtattcatg ataaacatga
    36601 tatagattca attcatattc tttcttagcg ttcgtgacga ctgtatccaa aatcaaccct
    36661 gttactaatg aaagaagtgc taaagtcatc aatccagtac ttaaaatcgc agaagggatg
    36721 cgtgtaataa agcttgtcag catatattcg cgaatcacag gaatccccgt gaccaatcca
    36781 aataaaaaga agaagatggt ccaaatccca aagaaaagca aaggcttata atctttaaac
    36841 attttaataa tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg
    36901 ctgccttcag gacgatcacg ataatcgatt gggatttcaa cgaacttgaa ttttttatct
    36961 aaagcatgga tcgtcaattc cgtttcgatt tgaaaaccag aactcataat aggaaaactt
    37021 ttgacaaaga tacgattgaa tcctcgatag ccggtcatga catcaagaat ttttgtttta
    37081 tacattctag taattgtatt tctaacaagg ttatttccaa aatcgtggaa aggtctctta
    37141 ttctcatcaa agtaggttcc atttgatagg cgatcaccaa tgaccatatc tgcttcacca
    37201 gaacgtaatt tctcgagcaa accatgtaca gcttctgcag gataagtatc atcgccatct
    37261 accattaaat aatagtcggc atcgatatca aagaacattt ggcggatcac atttcctttt
    37321 ccttgacgag gttctttttt tacgattgct ccaccttgaa cggccaattc gtatgttcga
    37381 tcggtcgaat tattatcata gacataaata tctgcttctg gtaattctcg tttaaagtct
    37441 gcaatgactg ttgaaattgt tgcttcctca ttgtaacaag gaatcagaac agcaattttt
    37501 tcttgttgtt ccatttttca ctctccttaa ctatagaaaa caaaagctaa attaaaaata
    37561 cctcataatc aaaggaaatg ctagtttttc ccgctaaata tcatttattt aaaaagaaaa
    37621 tactggaata tatgtttgga aagctctatc taaaaaacca cccactacaa aagaaggaca
    37681 tgatacctct cttttgaata gtgggtgcgt attcgttctc tcactttttt gacgtattaa
    37741 tctgttgcac ctttttttct tgaaatttct aatacggttc ctgtatgagc atctgctaaa
    37801 aattcataat aaacagtttc gccatcttct attcgagaaa gacctccccg ataagcactc
    37861 gaccgaacgg cgaatttcct aataggttcc ttttcaaaag agatccaaga accttcgatt
    37921 gcaccttctt ttagaaaaga tgacttgata ttttcaagta cttggtctgc tgacaatact
    37981 tttttgcgat gataccaaat cgttgaagct accccgctca ataatcctag accgattcca
    38041 actgctaacc cgcctttaaa atagcttagt tcgctttctt catacatgtg gacatacctc
    38101 cttcttctca cttgttactc cttgtgctta ttttaccaca ctctcatatt ttctttgaaa
    38161 taaataaccg ctaccagaaa aaataagcta taataaatac gatcggtctg cagaaaaaat
    38221 catattttat ccgcagatca atataagtct gagaggagtt tagtctgaaa tttagacgaa
    38281 tagcatatgg aagaaaaaac atttcaacga atcaaagagt taacagaatt acaaggaaca
    38341 agcggttttg aacaagatgt tcgcgcttat atggaaagtc atatggaacc gctggtagat
    38401 gagttacagt tagatggttt gggcggcatt tttggtctgc gccatcatga agaagcggat
    38461 gcaccccgtg taatggtcgc agcacatatg gatgaagtcg gattcatgct gacacaaatc
    38521 caagaaaacg ggctttttaa agtcgtacct ctaggcggct ggaatcctta cgttgtttct
    38581 gctcagcgtt ttacacttaa aacctctact ggtaagaatt atccatgtat ttcttcttcc
    38641 gttcccccac atttattgcg aggaacaagc ggacaaaaat cagtagaagt aaccgatatt
    38701 ttgttcgatg ctgggtttga atcaagagaa gaggcaatga gttacggtgt ccttccagga
    38761 gacacaatcg ttccttacgc tgaaacgatc aaaacagcca acggaaagaa tattatcagt
    38821 aaatcatggg acaaccgcta tggctgtaca atggtattag aagctttaga agcgttgcaa
    38881 aatgaaacat taggtcatac attgattgca ggcgcgaatg tccaggaaga agtaggcttg
    38941 cgtggttcta aagcttctgt caataaattc aagccagatc tgttctttgc agtagactgc
    39001 tctgcagcag atgatacagt gacgaaaaat ggcacattcg gacatttagg agaaggtaca
    39061 ttgatgcgta tccaagatcc aggtttgatc atgcttcctc gtttgcgcga gtacttgctg
    39121 gatatcgctg aaacaaacaa tatcccttat caatactttg tttcaaaagg cggtacagat
    39181 gcaggtgctg ctcatacaca aaacgaagga atcccaagta cagtgattgg cgtagtggga
    39241 cgttatatcc atacgcatca aacgatgttc agtattcgcg attttgaagc tgcacgagaa
    39301 atgttgattc aaacactgaa aggtttggat aaatcaacag tgaatacgat cgtatacgga
    39361 aaataatgcg atagtgaaac tataaaatct ggcaaaagat tgctttttgc cagatttttt
    39421 ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt taattgaaaa
    39481 atacataaac agaaagtaga ggacaaagaa tgattatccc aaatagttta gaagaattag
    39541 caggctacgt agaaactgga aagagtgtat ttttctttac tgccggctgg tgcggagact
    39601 gccgttttat caaaccgcaa atgcctgaaa ttgaaaatga cttttctgat tggcaattta
    39661 ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt tttggtatcc
    39721 caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat aaagatcgta
    39781 aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaac aaaaggctga catctgttag
    39841 gaggggtcaa gatgaatcaa gaatataaga atattttagt aggtatcgac ggtagtgaac
    39901 aagcacttgc tgctttcaaa aaagcggtag aagtggctcg ccgtaataat gggaaagtat
    39961 atgtagcaaa cgtaatcgat cagcaatttt ataactttat gggctatgca cctattgatc
    40021 aaaatctagt cgatcagcaa actgaaacag ccaaggaaat gatcgaagaa tgtaaagaat
    40081 atgggaaatc tgttgattac acaaatatag aaggaattat tgcatatggt tctgttcgtg
    40141 aatccatggc agtcaaattg ccaaaaaaat atgagatcga tctaatcatg gttgggcagt
    40201 caggtctaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac gtgatacgtc
    40261 attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt aattaagcaa
    40321 gtggaacttg tacgaccttt ttaatcaatt aactaaaaca gtaaagggac tgtgacaaaa
    40381 gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaggctt cttcggaaat
    40441 aaagcatgaa aaaaatttca tgcagtgaat gcaatttttt gttaagataa atctcagcaa
    40501 aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc ggagacacct
    40561 tgatggttat tatagccgac gataaaggaa cagaaaacac tgttgaacgc aaatggaatg
    40621 ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc catatctccg
    40681 atcatctaac aatcgaaggg aacggccaag taagcttaac agatgaggaa ttagcgaaat
    40741 tgaatcgatt gatcaaagaa gcaggattcg aagaacagct tgaagctgat catgaaccga
    40801 agatcgttgt cgggtacgta aaaacatgcg tcccacatcc tgattctgac catttatcga
    40861 ttactgagac agaagtagac aatggtacag tattgcaaat cgtttgcggt gcacccaata
    40921 tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc tatgatgcca gatggaatga
    40981 tgatctggcc aggaactttg cgaggggtag atagctatgg aatgatctgt tccgctcgtg
    41041 aattgcattt gccaaatgct ccagcaaaaa aaggaatctt agtattgcct gaggatgccg
    41101 tagtcggaga agcatttcca aaataaagag gttgtgacaa gtctttgtca caacctcttt
    41161 tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc gtcaattcaa
    41221 cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa tctcccactt
    41281 ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg tcatcgatct
    41341 tagtaatgac gtcgtattgt ttcaatccag ctttttcagc cggtgtagct gtttggacat
    41401 ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt tcttgttgct
    41461 gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt gtcacttttc
    41521 cgtctttttc cagttgattg atgatattca ctacatcgtt acttggaata gcaaatccca
    41581 ttccttctac gcttacatta gaagtagatt cagaagtact tgcgatctta cttgagttga
    41641 taccgataac ttgtccttcg atattaacta aaggaccacc agaatttcca gggttgattg
    41701 ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc gtacttgtca
    41761 cttgtcggtt caaggaagaa atgattcctg aagtgacaga atttgcatat tcagatccta
    41821 gtggggaacc aatcgcaatc gcaggctcac caaccttcag agcacttgag tcaccaaaag
    41881 aggcaactgt ttcgactttg tctgaattga tcttcaacac cgctaaatct gaataagcat
    41941 ctgttccaac taattcagct tttaccttcg taccgtcttt catcaagact tcaagacctt
    42001 gctggccatc tactacgtga ttattcgtga ctacataagc agtattcccg tcttttttgt
    42061 agatcacgcc gcttccttca ctataagctt ccaaattact atcatctgag ctgctacttt
    42121 cttcttgctg cccgaatagt ccgccaaaac cgcttgattg gttttgactt tgtaagttga
    42181 taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca gaatctacat
    42241 tgactttgac attttccaca actgtttcac cggcagaatt ctgattgccg cttgtagcag
    42301 tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc agtagtcctc
    42361 caacgatccc tccaacaagc cccagaccta acttacgcca gaggctatta tttttacttt
    42421 ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat tacattcatt
    42481 tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac tgtgaacttt
    42541 ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat tttctttttc
    42601 cacagatact gtgtaaaact acgcggatag gattggaaaa ataagggata aaacatatcc
    42661 acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta atgaatgtga
    42721 tgaaataagg taaaataaca ctgttttctg tggataaacc tgtggatgtt gtgcataact
    42781 gttggggaaa tctttttaac aaaaatagcc aaaataaaca tatagacgaa aaaattgatt
    42841 ataataaaca aagtgagcat gaagaccaaa ggagagaaaa catgaatatc aaaattatct
    42901 cagtaggaaa attaaaagaa aaatacctga tccaaggtat caatgagtat gtcaaacggc
    42961 tgaatgccta tgcaaaaatc gaattgatcg aagttcctga cgaaaaagcc ccggagaatt
    43021 taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg ggaacggatc ttagcgaaaa
    43081 tcaaagaaca agaatacgtc tatgccttag caattgaagg gaaaaatcct acaagtgaat
    43141 cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt gtttttgtta
    43201 tcggcggctc tttaggactc agcgaagccg tcatgaaaag aagcaatgct cagatctctt
    43261 ttggaaaaat gacgtatcca catcaattga tgcgtctgat ccttgtcgag caaatctacc
    43321 gtgccttccg gatcaatgcg ggggcgcctt atcataagtg atgcggtttt tatcattgat
    43381 cctaaaaatc ttagcaagac aaaaagtacg taagaaagta aagactaaat acttcggagg
    43441 aacaaatgaa taacaatgat aggttattgc gattgcgtta tgcgattgat ttgaaagata
    43501 cggatttgat taaagctttt gagctaggcg gtgtaccact gactaaagaa gaagctcagg
    43561 cggtactgac caaagtacag gataagaaca aagataatgc agaaaataat gtgtatgaaa
    43621 aaaccatcaa caaccaagtt tttgacgcat tcctaaatgg cctgattctc ttggcgcgtg
    43681 gtccgcaaaa agataagccg atagtagaca cgccgcaaaa aagcaaggaa atcaaatata
    43741 tcaacaatgt gttgctaaaa aaattaaaaa tcgccttgtc tatgaccact gatgatattt
    43801 tagacgtttt tgctgaggca gaaatctatc catcaaaagg cgaaatcggg gcatttttga
    43861 gaaaagaagg ccaacgaaat tttaagcctt gcggagataa atatatgcgt aattttctca
    43921 aggggttagg aatttataat cggagaaaag tataaagaaa tttcatgaga aatcaaaaca
    43981 atggttgttt tgatgagagc tagtttttgt atgagattaa aatattatct taatcgagga
    44041 gctggattgg ttttatgaat gatgaatata agaatgatga agataagatg ctttttgaag
    44101 agatagagaa tcgatgtagg ctcaattttg agcttcgggg aaagatgtct ttaatacaac
    44161 aaaaaaagta tttagcgaat aaatcagagt ttactctggg acatgttgaa aaattaatct
    44221 cggattggat tagtagtaga agcgaattta caaaaataaa acagccgatt aaatttgaca
    44281 tgaaaaaact tttgttaaat aagagtgaga taggtaatag agatcaatat attagagcta
    44341 aagggcaaga aataattgat tcattaggtg aaatgaggtc atacaattat ttgtatgtaa
    44401 cacatagagc tgatggaatg gtcattactg tggggaaatc ttcttctaat gatatttttt
    44461 tagatggaga tcttttttat caactcaata caaatcactt gtctggcact gaaaatataa
    44521 ttttaaggac ggaatatggc aatgaaatat ttgcaaaata tgatgagatt ttaaaaaatt
    44581 atttggactg ggcttggata attccggttg agtcaggaga tgctaagaaa ctggagagat
    44641 tattgggcga tgaactaatt aataaaaaag taccaatttt aaattattat tcccatagac
    44701 aataaaaggc tatttttcat gataatacta gttataactg taaaaatgga agaaaatctg
    44761 ggaaagaagt ttttaaataa gtgtattgtt cgattttaat aaataataag caataccatc
    44821 cgtctgtgtt gcgcaacatc gtactacaat gaatattttt tcacaaaaag aaaatcatcc
    44881 atcgaatgaa tggaaggttt tagtatacac gaggttaatg ccccctcttt tataatcaga
    44941 ggagtgaggt tttttctatg aatgtttcta ttttcttatt gctagtctag tttctcttac
    45001 gatgaaattg ggattctatt ccagttttta ttgatttgct gataaaataa cttgaatggt
    45061 ggatataaac atgataaata agagaagaat aacaaaacaa ggttgtgaaa tgattctcaa
    45121 atgaatataa tagaaagaaa aaggaggaag aatcaatgaa aatagcaatt gcaggcgctg
    45181 gcgcaatggg aagtcgcttt gggttaatgc ttcaccaaag tgggaacgaa gtattattaa
    45241 tcgatggctg ggcagaacat gtccaacaaa tcaaagagca tggattacaa gcaaatttta
    45301 atggtaaaga ggtagaagca aaactaccaa tcgttcttca atccgaagta gaaaaagaag
    45361 atcaagttga tctgattatt ctatttacca aagcgatgca gctggaaaaa atgctgcagg
    45421 atatccaatc attaatcaaa aaagatacag aggtcttatg tctattaaat ggtatcgggc
    45481 atgaagatat tattgagaaa tttgtaccaa tggaaaatat ctatattgga aataccatgt
    45541 ggacggcagg tcttgaaggt cctggtcagg tcaaattatt tggaagtggt tcagtagaat
    45601 tacaaaattt aggtgatgga aaagaagcag ctgcaaaaaa attagcagac aaattgtctg
    45661 aatcaggatt aaatgctcat ttttctgaca atattcacta ttctatttat cgcaaagcat
    45721 gtgtgaacgg aacaatgaac ggactatgca caatcttaga cgttaatatg gctgagctag
    45781 gaaaaacatc gactgctcat aaaatggtgg cgacgattgt caatgagttt gccaaagtag
    45841 cagcagtaga gaagattgaa ctagatgtcc cagaagtcat tgcacattgt gaatcttgtt
    45901 ttgacccgga aacaattggg ttacattatc cttcaatgta tcaagacttg attaagaacc
    45961 atcgattaac agaaatcgat tatatcaatg gcgcaatttc tagaaaaggg aagaaatatg
    46021 gtgttgcgac gccttattgt gatttcttga ctgagcttgt ccatgcaaaa gaagatagtt
    46081 tgaacgtaaa ataaaggaga gattgaatgg aaacttcaca aaaaatatca ccgaaaattt
    46141 ttctgaataa agtcctcgct ggtacagcaa caggaatcat tattggattg attcctaatg
    46201 cagtattagg tgccatttta aaatatttta gtgcgtatcc ctttgcggta acgatcgcac
    46261 agttagcagt tatttttcaa ctagcaacac ctttgatcat tggtggtttg attgccttac
    46321 aatttggatt tgatccgatg agaatgatgg ttgtagccgg agcttctttt gttggttctg
    46381 gtgtagtcaa attcacacct gatttgggtg aaacgggtgt ttacgttggt gctggtacag
    46441 gcgatattat taatacgatg ctgacagcat cgattgctgt cgggctgatt ttactgattg
    46501 gaaatagttt tgggtcagtg tcgattgtgt taacaccgat tgttgtaggt gtaggtgcag
    46561 gtttacttgg tttttattta ttcccttttg taacagcaat tacttctgca attggatcat
    46621 tgattaataa cttcactacc ttacaaccgc tgttaatgtc tatcttgatt ggttgttcct
    46681 ttgccttttt gattatctct cctatttcaa cagtagcaat cggaatggca atccaactag
    46741 atggtgtttc tgctggtgcg gctgcgatgg gcgtagctgc aaccactttt gttttagtag
    46801 tgaactcttg gaaggttaat aagtcaggtg tgacaattgc gattgcttta ggcgctatga
    46861 agatgatgat gcctaatcta tttagaaaac ctgtgattct tattccttgt ttagttagtg
    46921 cagtgattac agctattcct gtcgctctat tttctatttc aggtacgcct gcttctgctg
    46981 gtttcggcgt ggtcggactt gttggtccgt tggcttcttt agatgcaggg ctaaatggct
    47041 ttcttgttgt tctttgttgg ttagtcatcc ctgtttttgc ggcactaatt actcaatttt
    47101 tatgcgagaa agtatttaaa ctgtatgatc gagaagaagt cttcaaattt ttaggctagt
    47161 agattttact aagaaatttt caatcaatgg atgcgtaagt gagatgtcaa caacatctga
    47221 cttacgtttt tttgcctttt tgagtaggag attgatgctg acaactgaac tcttcagtgc
    47281 cattcgattt tttaaaatgt agaagaaact cacctttatg ttattgattg ttcaaaaggc
    47341 aactagaata aaaatatgtg ttaaaatgag aaaaaatttg attgttggat gtatcgaata
    47401 atcactaaaa agtaggagaa gaaaatgatt tttttgcaaa aatatggttt ttatttttta
    47461 ttattaggtg tgattagtga tttatcgaca ccgtatatcc ttggcctttt ttatcccaaa
    47521 ctgaatcaaa tgactaccgt aattagtgtg tttggtgacg tagatagtcc agtcagacgt
    47581 gctttcttgg tctggtcagt tgtatctgga cttttttttg ttctttcgtt gcctgcgttg
    47641 tatcacctat ttattggaac atcaaaaaca cttgcaatac ttgctgttgc aacagttggt
    47701 ttatatggga taggtgattg tatttttact gggttattca gtatcaatac gaatgaaagc
    47761 tcttggaatc tatcgacttg gatccataat ataggttctg ggctaggcta tgcaggcttt
    47821 ctacttttcc cattgttact tgttctgctt tatcgtcaaa gtggtcaagg tacgcttagc
    47881 catttttatc ttgttttaac agttattagt ttactaattg caggtcttta cggattagca
    47941 cggattccgt cgattagtca gtttgccttc tttaagcaat tgggcttctt tcaacgctta
    48001 agttttttct ttaactattt gccgatgatt tgtttaagtt tttttcaact acgaaagtag
    48061 aaaaagcagt gtgaagctga ttaagtaaaa gaatttctct atgggtaacc attcgtgtgg
    48121 atgtgttcga ctaagaatag agatgaggag agcgaaaagc aagttagtct taacttgtta
    48181 tgcgttgtgc taaatttcag gtagtcattt tttgaatgaa taagaagaaa acttgaaaga
    48241 gggtgacttc atgttagatg tggagcagat ggaaatagac ctttataata ttattttaaa
    48301 tcgaaataca agagaatggg aaagaacaca atttgaagag gcaaaagaag acctagcaaa
    48361 aatgtcgata aagcaggtga tagaaaaact agaggtcaac ttgcgtccgc tagcactacg
    48421 agataatttg acaccagatg cgatggattt ttatttaaga atcacagggg agtcagcgag
    48481 tgcaaaggct tatgattatg ccaaacataa gataacttca cccttatacc aagaacgggc
    48541 gatttttgcc ggtggctgtt tctggtgtat ggttgagcct tttgaaacaa aaccaggtgt
    48601 aatctcagtg ttatctggtt atacaggtgg agagaccaaa aaaccaaatt acgatcaagt
    48661 cagtggtgga tatactgggc atgttgaagc tgttgagatc atatttgata cacgtcaaat
    48721 aacctatgaa gagctgttgg agatttattg gcaaatttca gaccctacag atgcctttgg
    48781 tcaatttcaa gatcggggga aacaatatcg ttcaattcta tttgttgtta atgaaaagca
    48841 aaggcaattg gctgaaaaat caagagaaca actgattcaa tctaaccgat ttagccgtcc
    48901 tatcgttaca gagattcgcc aagcaaagac tttttggcca gctgaaaatc atcatcaaca
    48961 attttatcag aagcaaccta aaagatataa aaagatcaag cattcgcatc agcttcattt
    49021 attttttcag cgacatagat agaggaaggt tgtggcgcaa agcgttcagc tccgacaaaa
    49081 taatgagcaa ctgacaaaaa tagcttttca tatttttgga cagttatgaa ttatttcgag
    49141 gagttgcttt gagaacaccg aggaaaaggt tgtggcgcaa agcattcagc ttcaagtcgc
    49201 aaggaaacag gaggtatgac aagtcttttg tcacaactcc tgttttttta ctagccttta
    49261 tttccatgtg aaagagaatt cgaattgtag aatcttgatt tttctatgga attttgtaga
    49321 gaatcatctt gataattaat agacacctaa tcgaattcca cattaatgat gaactaaatt
    49381 ccgtataaaa gcgcaaacaa taacta
//
LOCUS       DAZKAY010000009        46957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000009 DAZKAY010000000
VERSION     DAZKAY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.009"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1046
                     /locus_tag="ACG8WP_000479"
     CDS             <1..1046
                     /locus_tag="ACG8WP_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026817.1"
                     /translation="HSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNRTAVDKWEAD
                     SIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEGKINVSFFGI
                     DKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTFKLTGFTDHA
                     KFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQIKISKFINE
                     LPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGITGGFIARSV
                     VAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVVAVLGALFSV
                     RTIVKIDPLKAIG"
     gene            1061..1729
                     /locus_tag="ACG8WP_000480"
     CDS             1061..1729
                     /locus_tag="ACG8WP_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026818.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1749..2327
                     /locus_tag="ACG8WP_000481"
     CDS             1749..2327
                     /locus_tag="ACG8WP_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9026819.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2728..3639
                     /locus_tag="ACG8WP_000482"
     CDS             2728..3639
                     /locus_tag="ACG8WP_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9026820.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3727..4644)
                     /gene="rnhC"
                     /locus_tag="ACG8WP_000483"
     CDS             complement(3727..4644)
                     /gene="rnhC"
                     /locus_tag="ACG8WP_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9026821.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            4846..5283
                     /gene="zapA"
                     /locus_tag="ACG8WP_000484"
     CDS             4846..5283
                     /gene="zapA"
                     /locus_tag="ACG8WP_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9026822.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5342..5890
                     /locus_tag="ACG8WP_000485"
     CDS             5342..5890
                     /locus_tag="ACG8WP_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9026823.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6005..8365
                     /locus_tag="ACG8WP_000486"
     CDS             6005..8365
                     /locus_tag="ACG8WP_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9026824.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8452..8766
                     /gene="trxA"
                     /locus_tag="ACG8WP_000487"
     CDS             8452..8766
                     /gene="trxA"
                     /locus_tag="ACG8WP_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9026825.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            8948..10726
                     /gene="uvrC"
                     /locus_tag="ACG8WP_000488"
     CDS             8948..10726
                     /gene="uvrC"
                     /locus_tag="ACG8WP_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9026826.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            10871..11770
                     /locus_tag="ACG8WP_000489"
     CDS             10871..11770
                     /locus_tag="ACG8WP_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026827.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11763..13025
                     /locus_tag="ACG8WP_000490"
     CDS             11763..13025
                     /locus_tag="ACG8WP_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026828.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13186..15084
                     /locus_tag="ACG8WP_000491"
     CDS             13186..15084
                     /locus_tag="ACG8WP_000491"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9026829.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15414..16133)
                     /locus_tag="ACG8WP_000492"
     CDS             complement(15414..16133)
                     /locus_tag="ACG8WP_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9026830.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16323..16370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16470..16802
                     /locus_tag="ACG8WP_000493"
     CDS             16470..16802
                     /locus_tag="ACG8WP_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026831.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16789..18792
                     /locus_tag="ACG8WP_000494"
     CDS             16789..18792
                     /locus_tag="ACG8WP_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9026832.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18811..19281
                     /locus_tag="ACG8WP_000495"
     CDS             18811..19281
                     /locus_tag="ACG8WP_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9026833.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19302..19886
                     /locus_tag="ACG8WP_000496"
     CDS             19302..19886
                     /locus_tag="ACG8WP_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFP9026834.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            19901..20887
                     /locus_tag="ACG8WP_000497"
     CDS             19901..20887
                     /locus_tag="ACG8WP_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9026835.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            20877..21188
                     /locus_tag="ACG8WP_000498"
     CDS             20877..21188
                     /locus_tag="ACG8WP_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9026836.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21210..22991
                     /locus_tag="ACG8WP_000499"
     CDS             21210..22991
                     /locus_tag="ACG8WP_000499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9026837.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            22984..24360
                     /locus_tag="ACG8WP_000500"
     CDS             22984..24360
                     /locus_tag="ACG8WP_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9026838.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24367..25002
                     /locus_tag="ACG8WP_000501"
     CDS             24367..25002
                     /locus_tag="ACG8WP_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9026839.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25032..26387
                     /locus_tag="ACG8WP_000502"
     CDS             25032..26387
                     /locus_tag="ACG8WP_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9026840.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26579..27973)
                     /locus_tag="ACG8WP_000503"
     CDS             complement(26579..27973)
                     /locus_tag="ACG8WP_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9026841.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28065..29036
                     /locus_tag="ACG8WP_000504"
     CDS             28065..29036
                     /locus_tag="ACG8WP_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9026842.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29033..29815
                     /locus_tag="ACG8WP_000505"
     CDS             29033..29815
                     /locus_tag="ACG8WP_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP9026843.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(29984..30355)
                     /locus_tag="ACG8WP_000506"
     CDS             complement(29984..30355)
                     /locus_tag="ACG8WP_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9026844.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30489..30932
                     /locus_tag="ACG8WP_000507"
     CDS             30489..30932
                     /locus_tag="ACG8WP_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9026845.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(30975..31784)
                     /gene="yidA"
                     /locus_tag="ACG8WP_000508"
     CDS             complement(30975..31784)
                     /gene="yidA"
                     /locus_tag="ACG8WP_000508"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9026846.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31803..33170)
                     /locus_tag="ACG8WP_000509"
     CDS             complement(31803..33170)
                     /locus_tag="ACG8WP_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9026847.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33154..33984)
                     /locus_tag="ACG8WP_000510"
     CDS             complement(33154..33984)
                     /locus_tag="ACG8WP_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9026848.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34147..34584
                     /locus_tag="ACG8WP_000511"
     CDS             34147..34584
                     /locus_tag="ACG8WP_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9026849.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34660..35280
                     /gene="rpoE"
                     /locus_tag="ACG8WP_000512"
     CDS             34660..35280
                     /gene="rpoE"
                     /locus_tag="ACG8WP_000512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9026850.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35469..35975
                     /locus_tag="ACG8WP_000513"
     CDS             35469..35975
                     /locus_tag="ACG8WP_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9026851.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            35992..36891
                     /locus_tag="ACG8WP_000514"
     CDS             35992..36891
                     /locus_tag="ACG8WP_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9026852.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            36978..37862
                     /locus_tag="ACG8WP_000515"
     CDS             36978..37862
                     /locus_tag="ACG8WP_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9026853.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38054..38125)
                     /locus_tag="ACG8WP_000516"
     tRNA            complement(38054..38125)
                     /locus_tag="ACG8WP_000516"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38090..38092),aa:Arg,seq:ccg)
     gene            38185..39519
                     /gene="rpoN"
                     /locus_tag="ACG8WP_000517"
     CDS             38185..39519
                     /gene="rpoN"
                     /locus_tag="ACG8WP_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9026854.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39668..40759
                     /locus_tag="ACG8WP_000518"
     CDS             39668..40759
                     /locus_tag="ACG8WP_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9026855.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            40800..41801
                     /gene="gap"
                     /locus_tag="ACG8WP_000519"
     CDS             40800..41801
                     /gene="gap"
                     /locus_tag="ACG8WP_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9026856.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            41911..43101
                     /gene="pgk"
                     /locus_tag="ACG8WP_000520"
     CDS             41911..43101
                     /gene="pgk"
                     /locus_tag="ACG8WP_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9026857.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43205..43960
                     /gene="tpiA"
                     /locus_tag="ACG8WP_000521"
     CDS             43205..43960
                     /gene="tpiA"
                     /locus_tag="ACG8WP_000521"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9026858.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44076..45374
                     /gene="eno"
                     /locus_tag="ACG8WP_000522"
     CDS             44076..45374
                     /gene="eno"
                     /locus_tag="ACG8WP_000522"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9026859.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            45773..46912
                     /locus_tag="ACG8WP_000523"
     CDS             45773..46912
                     /locus_tag="ACG8WP_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9026860.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        15593 a         8141 c        10022 g        13201 t
ORIGIN      
        1 tgcattcaaa gttacgttat gcgttagttg caggggtgat gtttttgatc gcctacctgg
       61 tatttttttt gactgggtta gcttatggct tagctcaaga taatcgaaca gcagtcgaca
      121 agtgggaggc agattcgatt gtgctttcga aagatgcaaa ttcaaatcta gggatgtcca
      181 tgattactaa gaagatagca gaagaagtcg aaggtgggaa agtggcgtat cttgcccaaa
      241 cacctggagt cgtgactagt aaagacagta cagaagaagg aaaaatcaat gttagcttct
      301 tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt tgaaggaaaa gcttttgata
      361 acgatgatga ggcagtaggc gatattagtt tgaaagaaga atatggtcta gccataggag
      421 atacagttaa gttatctggt agtgacaaga cgtttaaact aactggcttt acggatcatg
      481 ccaaattcaa tgtctcacca gttctctata caaccatcaa tgcttaccaa gaaatccgct
      541 tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc aatagttgtc agaggtaaaa
      601 tcagtaattt acctgaagat ttagaacaga tcaaaatttc taaattcatc aatgaattac
      661 ctggatataa tgcgcaagta ttgacttttg gatttatgat cggatttttg atcgttattg
      721 cggctattgt gatcgggatt ttcatttatg tactgacgat gcaaaagatt aatatctttg
      781 gtgtaatgaa agcacaaggg atcacaggag gatttattgc gcgttctgta gtggcacaaa
      841 cattcatttt atcatttgtt ggtattcttc ttggtctatt aggaacagtg ggaacatccc
      901 ttgttctacc tgatgcggtt ccattccaaa gcaattggct gttttttggc gtcattagct
      961 tgttgatgtt agtagtagct gtgttaggcg cactattttc tgttcggaca atcgtaaaaa
     1021 tcgatccgtt gaaggcaatt ggataagaag gaggaagaac atgaaagcaa tagaatttaa
     1081 agaagtagaa aaaagtttcc aagatggcga ccaagtagtt aaagcattga aagaaacaac
     1141 catgagtatc gataaagggg aatttgttgc aatcatcggt ccttctggtt caggtaaaag
     1201 tacttttttg actttggcag gcggattgca gacgcctagc agtggctctg tatttatcaa
     1261 tgatcaagaa ttcagtaatc aaaaagaaag cgaacgcgtt aaactacgtt ttaaagaaat
     1321 tggctttatt ttgcaagctt ccaatctggt tccttttctg acagtaaaaa agcagttatt
     1381 attagtagat aagattaaac atgaacatcg tcaaaaagaa gcaaacgaat tatttgagca
     1441 gcttggcgtg gataagttga tcaacaagta tccagaagaa ctatctggtg gagaacggca
     1501 aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc attattctag cagatgaacc
     1561 aactgctagt ttggattcag aaaaggcacg agaagtagtc gatatcttgg caaaagaatc
     1621 aaaagacaaa aataaagcaa cgatcatggt tactcacgat acacggctga ttgacaaatg
     1681 cgatagaata tttatgatgg aagatggtgt attgaaagaa aaagaataac caaaaagcag
     1741 gtggaaaaat ggtccatcga tacaacaaag atacacagca aaaaattttg aaagaagcga
     1801 ctgacttgtt tatgtccaag ggatatctcg gcacttcaac aagagaaatc gcacaaaaag
     1861 ttgggatcac ccagcccaat ctctatcatt atttcggtga taaagaaaaa ctatatacgg
     1921 cagttttaga aaaccactta aaagatgttg gaaaagcttt gagagaaatc gttcaaacaa
     1981 gcgaaacggg atttcaagta accttgacaa aaatggcgca gtttttgatt gagacacatc
     2041 tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa tctttcaaag gaaacaagag
     2101 atcatctttt ctttctatgg aagaaaaatt atcgtgaacc ttttgaagaa atcttttcag
     2161 caaaccaatc tgtgctaaga aatgggattt caaaagaaat cgcagcaagg cattttttct
     2221 tagtgttagc accttacatc actcggtcga aaagacctgt ggaagaaccg ctaaatgtgg
     2281 cgcaattgat cgatttatat ttgaatggtg tattaggaga agcgtaaagc tgttatatag
     2341 ggagaaaata gttaggtgaa gaagcaagat atgacgaaga tcgttatatc ttgcttcttt
     2401 atataggtag tttttcataa agacaaattt gttattctct catgattatc agggtatatt
     2461 tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg
     2521 tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga
     2581 gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg
     2641 ttatatcttt tacagcccct tttttgctta cttaagctga ttttaagttt tggcccttag
     2701 tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca
     2761 atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga
     2821 caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca
     2881 gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg
     2941 ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca
     3001 aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat
     3061 ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt
     3121 gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca
     3181 gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct
     3241 ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc
     3301 gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa
     3361 tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta
     3421 gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact
     3481 gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat
     3541 atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct
     3601 gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa
     3661 aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa
     3721 caccgtttat ttttttagta gcttcaaagc tttttctgta tttgcaaagt gaagtttggc
     3781 atatttttca agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga
     3841 tttcgttcca gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc
     3901 ttccaaaaag ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc
     3961 tcctttagta acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt
     4021 tttaaaattt gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc
     4081 gatttttttt agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc
     4141 attgtatttt ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg
     4201 gatggttttt tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga
     4261 atctctaaca ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca
     4321 tactgttagc ggaccgaaat agctgccatt tccgacctca tcactaccta aaacggataa
     4381 ttgactgata ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg
     4441 tgcctccccc catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc
     4501 ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat
     4561 ctgtttattt aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt
     4621 tgctttgata atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg
     4681 caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat
     4741 catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat
     4801 ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa
     4861 acacgctaca aagcggtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag
     4921 catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta
     4981 tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat
     5041 caattgaaga aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa
     5101 aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac
     5161 gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg
     5221 gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat
     5281 taagatcctt ttatcaaaat aagaaaaatt taaaaaaagt tagaagagaa aggaacgtag
     5341 catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg gagcacgtcg
     5401 cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta ttgctgccca
     5461 gcatttttac aaaccattag caagtcattt ggaattatac attccatacc cagctgtcac
     5521 gccaacatca aagttagcat tttttgatca ggtatttgcc tttcacctag atgaagcttt
     5581 ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta cccgatttgt
     5641 gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag actggattgc
     5701 aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt tgcagttact
     5761 tgcctttgtt ccattagatg tggtccaaaa tcaattcggc gagcatagtt tcgctcgctt
     5821 tatcgttgag agaacgccat ttcttaccaa caagatgtat gagctttggg taacgaatat
     5881 tttgaattaa ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagattattt
     5941 aagaaggctg cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga
     6001 taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag
     6061 acaatttgta gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagcga
     6121 ggaacaaacg atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct
     6181 gcgaggcggg atccctatac ctaaacttga aaatatccgt ccccatatga aacggattga
     6241 gatcggagca gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac
     6301 aagcgaattg aatcgtttca ttgatgatct ttatgacagt gaaatcgaat ttgcacgttt
     6361 gtatatgtgg gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc
     6421 aatcgatgaa gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca
     6481 gaacattcgg cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa
     6541 aaatgcaaag tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc
     6601 agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg
     6661 acagacttta tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata
     6721 ccaaatcgct gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt
     6781 tccccatcgt caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa
     6841 tgcaaaagca cgatttggta aagaagtaaa agctatcgtg ccgggaatca gtgaagacaa
     6901 tcatgtcgtc ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa
     6961 tgacatcaca atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg
     7021 gaaaacgatc acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc
     7081 gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg
     7141 agacgaacag tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt
     7201 agatatttta tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg
     7261 aacagatccg caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa
     7321 gtctgcttat gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatataatcg
     7381 ggcaaatacg attaatgcca gcatggagtt tgatgtggat acattaagtc caacgtatcg
     7441 tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct
     7501 tgatacagaa gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa
     7561 cgaaatgatc acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat
     7621 gcgtcatttt gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta
     7681 tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa atgaagtcgt
     7741 gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca
     7801 gattgggcaa ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa
     7861 tctacatcaa gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa
     7921 aacgttgaaa ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt
     7981 acgtaaaaac gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga
     8041 agacgagctg acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt
     8101 acgatctgaa tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga
     8161 agaagcgcta agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca
     8221 agtgactatt gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct
     8281 taaaaaccat cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg
     8341 tgcgacaatt gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca
     8401 agtatagaaa tgctatacta gtcatagatt ttaaaaatgg aggtctttga tatgtcacaa
     8461 gtaattacag ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt
     8521 tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa
     8581 gaatatgatg aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact
     8641 cctcaacaat tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta
     8701 gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat
     8761 ttataaaaac ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg
     8821 tttttttgcg gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt
     8881 caggctggat tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga
     8941 gtaccagatg aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg
     9001 ttacttgatg aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa
     9061 aaatcgtgtc cgctcatatt ttactggtag ccataatacg aaaacggaac ggcttgttag
     9121 tgaaatcgaa gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga
     9181 gatcaatctg ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac
     9241 ctatcctttc cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaggt
     9301 actaaaagac aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac
     9361 aaagaagatc ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga
     9421 gccttgtctt tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc
     9481 gaaagtctat acgaaaatcg ttgatgaagt gaaaaacttt ttaaacggag gacacgaaaa
     9541 aatcgagtca gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa
     9601 agctgcagaa taccgagacc agatccgtgc cattgagaca atcatgactc gccaaaaaat
     9661 gacaaatact gatttactgg atcgagatgt atttggttat gcgattgata aaggatggat
     9721 gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt
     9781 tcctttctat aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga
     9841 aaatgagcat ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt
     9901 agaagctttg ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt
     9961 tgaattggcg agtaagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag
    10021 aaaacaggaa cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac
    10081 cccaatacgt atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc
    10141 gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa
    10201 aacagtcaac ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc
    10261 gcgagtgatt cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg
    10321 acaagtagat gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc
    10381 tggcatggca aaaaacgata aacataaaac aaatgagttg ctattcggta cagatttggc
    10441 tgtagtaccg cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga
    10501 agtccatcga ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc
    10561 ccgattagat gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt
    10621 caaatcgttg aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc
    10681 gaaaaatgtc gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt
    10741 acaaagaaag taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt
    10801 tggtaaataa taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag
    10861 gagtgaagaa atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt
    10921 cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg
    10981 ggctgggaag acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga
    11041 agtgttatgg aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc
    11101 ggaggaacgg ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga
    11161 acttagaggg aaatcaagaa aagagattga accaaaaatc gacttttgga tggaaaaatt
    11221 tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa
    11281 agtccagctg atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt
    11341 cagcgggtta gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa
    11401 acatggttca tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga
    11461 tcatttgatc atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg
    11521 tgaatcattt ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga
    11581 agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt
    11641 agaagatcct gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc
    11701 tatgtttaac cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat
    11761 cgatgcgtaa gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt
    11821 ctttcttgat catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa
    11881 actttgctca acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa
    11941 tcagtcagca aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg
    12001 aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa
    12061 ctgagaatga taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac
    12121 aactactgat acaacaacaa ctgactggct tgcaatcgat gcttcgagcg agtcaattag
    12181 gcatttcacc agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag
    12241 tcaattttga ttcaaaagga aaaatgacag tcggagaaga taactcgaca gtccaatacg
    12301 tcgtaagtta tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca
    12361 ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta
    12421 cgactgcaca aaagcatttt tacggaaaat taacaggtgt tttacttgtt gcattaacgc
    12481 aaatggcatt atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa
    12541 tggtcaaaaa tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt
    12601 attctcttct attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg
    12661 gctctttggt caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat
    12721 ctctaggcgg atacatgctt ggattgatac taggagcttc agatccaaac aatatcgtta
    12781 ttcggatcac ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa
    12841 atgagacagt cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat
    12901 tcttattgat gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtt tatagcgaag
    12961 gcggattatg gagttcattg aaacaatcga tttcaattat gaggaatgaa cgaaaaaaag
    13021 cataaaacaa tcctaaaata cagaaggaag ctggacatct attgttccca gcttcttttt
    13081 gtatagacct attttattta ttttactata ttggatatat attcattgaa aatagtgtat
    13141 aacaacttta taatattgag tataccaatt tttggaggcg ttttaatgga ccgttattta
    13201 gatttacttt cagaaaaatt cccctcaacg gaggatgtca tcacggaaat catcaatctc
    13261 gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagtgatat acatggcgaa
    13321 tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa
    13381 gacctttttt ctgaacaaat gacggaaacg gatatcagcc aattcactat ttttacggct
    13441 tatccagaat atgcattaac gactgattgg tacaaagaac aagataaatc gcagctgatc
    13501 catcatttga tcgatctttt acggtttacg acagtcaaat atacccggtc aaaagttcga
    13561 aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgaccgt
    13621 atcaatggaa aaaaagacta tgtaaaaaaa atcattgaac aacttgtttt gatgaaggaa
    13681 gaagaaaaat ttttaacgaa acttgcacag acgatccaaa aatctgtcat cgatcatttg
    13741 catatcgttg gagatatttt tgaccgagga actcaagcag ccaaagtgat ggatcgaatc
    13801 atggattttt cttctgtaga tatccaatgg ggaaatcatg atattttgtg gattggtgcg
    13861 tattgcggct ctgaggcatg tttactgaca cttttacgga ttgcagcacg ctataattat
    13921 ttatttgaat tagagaaaga atatgggttg aatttacgtc cattgttttc ttttgctcat
    13981 caaacttatc agaagaatcc agtatttgcg cctaaaaaca gtaaaaatct atccgaaaga
    14041 gagcaagaag aattagaaaa aatccatcag gcactttcta tccttcaatt caaatcagaa
    14101 catcagttat tggatcgtcg acctgaattt aaaatggacg atcgtaaatt gctggaaaaa
    14161 atcgactatg aagcatcaac gattgattta gaaggtcagc tctatgagtt gaaaggaact
    14221 tgttttcaaa cgatccagcc agatgatcca aaaaaattct tggaagagga acaaaaagtg
    14281 attgatagtt tgatgtattc attccagcac tctttgagaa tgcaaaaaca tatgacgttt
    14341 ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtgctgtt tcatggctgt
    14401 atacctgtag acgagaaggg acagctctta gcatttgaat tagaccaatc ttatcgcgga
    14461 aaagaactgc tgggcttttt tgaaaaacag ataactgaaa gcgcaaaaga tctgactgca
    14521 aaagaagatt tagcaaccga tttgatttgg tatgcgtgga gtgggccatt ttcgccttta
    14581 ttcggcaaga gtaaaatggc gacttttgaa cgttattttc tcacagagcc tcatacgcac
    14641 gtcgagaaaa gcaatcctta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt
    14701 ttagcagaat tcgacacaaa agaagaaggg tcagcaatca ttaatgggca cacgccagta
    14761 aaagtaaaaa aaggggagtc accgatcaaa gcaaatggaa aagtatttgt cattgacgga
    14821 ggtttatcca aagcttatca aaaaacgaca ggaattgccg gttattcact attatgcaat
    14881 tcttatggtt tccaaatcgt gacacattat ccgtttttgc ctattgaaca acaatttgca
    14941 aaaaaatccg accaaacaaa tgtgaaaaaa gtaatcgaac agcaactacc acgcaaactc
    15001 aatcgtgata caacaaaagg tatggaactg cagcagcaga ttacccagct caaaaggctg
    15061 ttggactatc gaaaggacga ttgagaagaa ttccagtgaa cattgttcca tagactggag
    15121 actgagacaa aagtcgtgtc gcagtctctt tttccggata aacggtgttc ttctgccaac
    15181 tcctcggtca caagtcacac tattcaacaa acatgaaaag ctgttttttg aaattgctcc
    15241 ttgtgactca gagttgaacg gcaggctcac gacctcttgg tataaacggt gttcaaaagc
    15301 cagtttctcg gtattaagcc aaatttatgc aaaaatgtga aaagccattt tcacaaaatt
    15361 tttcttaata ctcgaaacta aacagctttt tcacaacccc ctcagaaagt ctattaaaat
    15421 tcattcacaa agaaatcgaa taagcgctgt tcagatggat gtacacggtg ggtaagttcc
    15481 ggatgccatt gaacaccgag gattttatgc atatcatctt tgctttgaac cgcttcaatg
    15541 agtccatcgt tagaaaaagc gatggctttt aataaaggtg aaagatcttt gactgcctga
    15601 tgatggtaag aattcacaaa atatggatcg gggagaaggt tgcttaacaa actgtctgaa
    15661 acaatcttga cttcgtgagt cgtaaattgc ggggcagtgg gctgttgttc atgttttact
    15721 gaccactttg gatataatga aagatcttga taaagtgtac cttccaaaac gacattcaat
    15781 agttggagtc cccggcagac tgcaaaaatt ggttttcttt gtttcaatgc ttccaagatc
    15841 agtgcctgtt caaattggtc tcgttgaata tttgtttccg ttagctttgg atgaggatct
    15901 tccatgtaaa gttgtggact gacatcttga ccacctgcaa gaataagttt gtcgatttgt
    15961 ccaatcagtt gaggagctga atctggagac atgactggca gaataagagg caatccttga
    16021 gcatctaaaa ctgcatccac gaatccttgc ggtgtatagc tcacttgatt tccttggaaa
    16081 gtatctgttg ctcgaatcag ttgatttccg gcaatgccga tgattggttt catttgtatt
    16141 ccccctaaag tagacttatt gtaacaagga tttaaaaaaa attaaactat gtaagacttg
    16201 cccctatact tatctgttct tgctgtgtta ctatctgtaa agagaaattt cgtgtgaaat
    16261 tcatgacgat agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa
    16321 aaggttgggc gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg
    16381 aaggggcctg cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag
    16441 catgtacctc ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa
    16501 tccgaaaagc agaagaagaa aatgaaaagc gtgctcaaac actgcggcag cagctgaaga
    16561 tctatgcaac agaaaaagag caggaattgg aaacgatcca aacaaactat cgacaacaac
    16621 taaaacagtt ggtggaagaa aaagaacaat ttgaaataca agaattagaa aaagaaaaaa
    16681 tgaaattgaa agcagaagca gacaaggtgc aagcaacttt gaaagatcaa tatcaaaagc
    16741 atcagagtca agcgatcggt gcgatcattg aaagggtgaa agaaacttat ggccgtcact
    16801 aagatggaaa aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca
    16861 gtacaaggaa tccaaaacgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag
    16921 tgggtggaga agtattttcc agataccacg attattgata aagaagcaaa attaagcgct
    16981 ttaggtaatc ggttgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat
    17041 agccgtcaaa agaaactcca tttgaaacgt caagaacttt ccttacaaga tttggaagcc
    17101 aattattccg aagaaaattt cataaataaa ttgaaagaag tccttgattt aaaagaaaag
    17161 tgggaacaac taactgaaga acgtgatcgt ttagaagaag aggaagaatg gcttcttcat
    17221 tggcaacagc tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg
    17281 gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag aactgagtaa gcttgatgac
    17341 gtttatatgg aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta
    17401 aaaaaatcag ttgctgttat agaagaaatt gctgggaaat acgggttcaa taaagaaacg
    17461 tatccttatg aacaaaatcc taaagaaaaa ctgcaagaga ccaagcagca attatccatt
    17521 gttcacgagc agcaaaagaa gataacgaca gcgttaggtc agtgcagtgg ctatatccaa
    17581 gaatttgaat gggtagaaga agtcacactt gccgttgcag aacgagaaaa aataaaagaa
    17641 cggtttatcc atgcgactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa
    17701 cagaatttgc tgcatgtgtt aaacgaaacc gtatcagaag aagatatata cgtcagtttc
    17761 gaaacaccaa actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg
    17821 cttgtcgaac cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta
    17881 gatccaacac cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat
    17941 gttggttatg gtttgctgat gctcattggg acaatactag ctcaaaaact acttgttcta
    18001 ccacgcggga tgaaacgttt cgtaaaattc tttgagattc tctcgatacc atcgatcatc
    18061 tggggattga tctacagttc atttttcgga cagtcattac caaaagaact gtttggcatc
    18121 cgcttgcctt tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc
    18181 gtgattttcg gtctgataca gattttggtt ggattatttg tggcagcaaa ggaaaatatc
    18241 aggagaaaag attacttgag tgctgtcagt gatggatttg cctggcaagg gatcctaatt
    18301 gggatcgtta ttgcgttggt tggtgccatg ttgataaaaa atacagcatt tgtttacctt
    18361 ggcgctgggc tatcgatcat tgctgcctta tgtatcttga tcgttccgat tatccaaacg
    18421 ccgtctaaag tcaaaggtgc tgccaaaggt gcgtataatc tttacggact gaccggctat
    18481 atcggtgatt tagtcagtta tacacgtttg atggcattgg gtatttcagg cggaagtatt
    18541 ggagcggcat tcaatatgct agttgctttc atgccgccag ctgctcgatt cagtgtaggg
    18601 atccttttga tcatcgcttt acacgcactg aatatgtttt tgacactgct aagtgcctat
    18661 gtccatggcg cacgattgca gtatgtcgaa ttttttggga agttttatac aggaggcgga
    18721 cgtgcctttg atccgctgaa aacggcggaa aaatacgtca acatcaatca caagaaaaaa
    18781 aacaataaat aattggagga tttttatata atgatggatt acttaattag tcaaaatggt
    18841 ggaatggtat ttgcggtatt agcgatggca acagcgacga ttttttcagg gatcggttca
    18901 gctaaaggtg ttggaatgac aggtgaggca gcagcagcat tgacaacaag tcaacccgaa
    18961 aaattcgggc aggctctgat tctgcaattg cttccaggta cgcaaggttt gtatggattc
    19021 gttattgcct ttttgatttt tatcaactta ggaaatgaca tgtcagttgt acaaggactg
    19081 aactttctag gtgcttctct gccgattgct tttacaggct tgttttcagg tattgcccaa
    19141 ggaaaagtcg cagcggcagg tattcaaatt ttagcgaaaa aacctgaaca tgctacaaaa
    19201 ggaatcattt ttgctgcgat ggttgaaaca tacgcgattt taggtttcgt tatttccttc
    19261 ctattagtgt tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa
    19321 tcatctctca gatgaacgaa gcagcagagc aggaaagagc ggcgttagaa caagaagagc
    19381 gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca agttgaaaca gaacatcaaa
    19441 aacaaaaaga aaaacaaatc gaattactcg aaaaaaaata ccgtcagcta aggaatcgcc
    19501 agcaagtaga agtacgacaa gaaaatttga atgcgaagca ggaattttta agacgattgt
    19561 ttgcagatgc ggtaactgaa atggaaaatt gggatgaatc tgaacaaatc cagtttatca
    19621 aaaatgcatt gtattcatta cctttgacag gaaaagtagc attcattgct ggagaaaaat
    19681 cagcagccta tctttctcag acgttgttgg atgaatggaa caatgaactt ccctttatga
    19741 tggttttaag tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag
    19801 ttcagtataa ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg
    19861 agattgccaa tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa
    19921 tcccttgatt cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat
    19981 tcaaacaaat tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata
    20041 tatttatgac ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct
    20101 atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg
    20161 ttatactttc cataatctga aggttttgac aaaagctgag atgacaaatc aaaatctgga
    20221 ccatttgtat atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac
    20281 gcaagtttca aatgaacttc ctgaaagcat tatggacagt atacgagaag tacgtgatta
    20341 ttttgaagag tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac
    20401 agagcagcga cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc
    20461 ttttattgac ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata
    20521 tgggttcatg acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa
    20581 ctttgtagat aaagatatgg aagattacac gaattttcta ttagagacag attatgctga
    20641 ttttctgcgc cctgtcatcc gtgagggaaa tattgatctc gtaaagcttg aacagttgaa
    20701 agatgattat ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc
    20761 actactagcc tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat
    20821 cggcaaaaga agccatttcg aatcagaaac aatcagaaag cgggtgagac aagtctatga
    20881 cgcataagat cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct
    20941 ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa
    21001 agaatgagta tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga
    21061 tcgaacgtta caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat
    21121 cattagggat agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata
    21181 tcttatagta tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg
    21241 gacctcttgt aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg
    21301 gagatctagg agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag
    21361 tatatgaaga gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt
    21421 tatctgttga attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat
    21481 tagatacctt tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg
    21541 cattggacca tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga
    21601 gtgctggaga tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg
    21661 tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg
    21721 aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat
    21781 ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca
    21841 ctggacagcg agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac
    21901 ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg
    21961 tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa
    22021 atgaattccc tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat
    22081 tgattgctaa tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa
    22141 tcacaattgc ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca
    22201 cttcacgatg ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg
    22261 atgaaggtta tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa
    22321 gagtcattgc attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat
    22381 ccccgtctgg aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag
    22441 tattctgggg tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga
    22501 tacaaagcta ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag
    22561 ctgactgggc atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga
    22621 atgaaatcgt gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag
    22681 aagtggcaaa atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata
    22741 cattcacttc aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag
    22801 aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat
    22861 cgattcgtga aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag
    22921 atcaaattat tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga
    22981 gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa
    23041 aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt
    23101 cgtcgtggac aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga
    23161 acaagtggta tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta
    23221 ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac
    23281 ggaccagaga tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta
    23341 gctcgggatt atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat
    23401 acattagttc gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa
    23461 ttagctgctc agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta
    23521 gtctttgctg ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt
    23581 caaacgggcg caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc
    23641 gaacgtatcg ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag
    23701 ggaatgcatg ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag
    23761 atttctgcag cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg
    23821 aacttagcta ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact
    23881 caaattccga ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact
    23941 ggttatatta cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag
    24001 ccaccaattg atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga
    24061 aagacaagag aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga
    24121 aaacaggcaa aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag
    24181 atctatgcga agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg
    24241 aaccgttcga ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga
    24301 accgaattaa aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga
    24361 gttctgatgg cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa
    24421 cagttgacaa ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg
    24481 cgtcaattca tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct
    24541 atgacagaag caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc
    24601 gaagaattat ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc
    24661 atgagtgtaa aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct
    24721 ttggaatacg ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact
    24781 accctgcttc caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg
    24841 agtgaaatcg aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa
    24901 ttagaagaaa cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta
    24961 acaaggttga tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa
    25021 agaggaaaac gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc
    25081 ttctcgcatt aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat
    25141 tttttagtaa tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg
    25201 ctgtctgtgt aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc
    25261 aatttttgat catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc
    25321 tatgctttgc attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag
    25381 cgctgaatct agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg
    25441 cctttgtgat ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg
    25501 gctggttgaa aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag
    25561 ggtttgattt gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg
    25621 tggtatctgc tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg
    25681 aatatcatca gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta
    25741 cccttttatt aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag
    25801 tggaagggaa tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa
    25861 cggctggata ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga
    25921 tgtttttgat gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta
    25981 cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag
    26041 catttggccg taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta
    26101 cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa
    26161 cttctggcat cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta
    26221 cgatgggatt gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt
    26281 acattggacg agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa
    26341 cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga
    26401 cagaaaccta gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata
    26461 ctttaagtgc aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta
    26521 gtgcctcttt tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt
    26581 ataattcata tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa
    26641 ggttattaga ggatagggaa attcgaacga atgcatctgg ttgcgtacgt gtagtaaagc
    26701 gtctggaatg ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt
    26761 caatctcagg atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta
    26821 ctttgaaaag agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta
    26881 agatcctatt tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag
    26941 aagttttcac atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa
    27001 ctacaagata agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga
    27061 ttgtttggtt aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg
    27121 ttaaaaatga atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg
    27181 ctaattgctc tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca
    27241 taagaaagac accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca
    27301 ttccttgctc gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa
    27361 tgaaattagc gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag
    27421 aaagtgtgat agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg
    27481 gaatgactcc ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt
    27541 tgggatcttg gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa
    27601 acgggacaac gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat
    27661 gttcggttac ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg
    27721 tgccacttcc aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc
    27781 caacggtagt aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag
    27841 tgtttttctg taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat
    27901 aataaggacg cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag
    27961 aattaatagg cattttctcc actccttaaa attgtatacc atacaatttt accattgatt
    28021 gtattgattt tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt
    28081 ttgaggaagt agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt
    28141 tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg
    28201 acggaaaagg caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt
    28261 ttggcacaaa agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt
    28321 taggagagat tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa
    28381 atgcagtcac ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa
    28441 atccgcatgc agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga
    28501 ctgcgatttc taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa
    28561 gacgaaatac ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg
    28621 caatatcagt agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta
    28681 aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca
    28741 catttactcc tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg
    28801 tagatgatta ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag
    28861 gagatttaga ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa
    28921 cattttataa aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag
    28981 atctttgttg tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt
    29041 agattattac gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt
    29101 ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa
    29161 tttatctgaa acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac
    29221 gccagatcga gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt
    29281 taactattac aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt
    29341 tgtgacaaga attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt
    29401 gccgatattg tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc
    29461 acgtgattta tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt
    29521 tacagctatc aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca
    29581 aaaagacttt gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg
    29641 tggatcagca cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt
    29701 gtcagcttat caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg
    29761 tgtgataata ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct
    29821 ttcaaatgaa ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt
    29881 aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg
    29941 tgtttgtttt ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt
    30001 cgattttata accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc
    30061 cttttaagtc agtaatctca agaccagcag ctttatgttg ctcgtgtaat ttttctagat
    30121 catcagctgc tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat
    30181 agttgtaagt caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa
    30241 atttgttttc tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa
    30301 attcgattga tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt
    30361 catctctcct tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg
    30421 tgcttgttca tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga
    30481 aactattcat ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc
    30541 gttatgcagc atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa
    30601 atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga
    30661 ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag
    30721 aagctgatga gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt
    30781 tctttgttgc tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt
    30841 ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag
    30901 tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc
    30961 agttttttta tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg
    31021 tttgatgaag tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct
    31081 actcctagac cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg
    31141 atttcatctt gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt
    31201 gcttctttat ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca
    31261 cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc
    31321 atcattttga cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc
    31381 atcttgacta aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag
    31441 atcgctttgt catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc
    31501 tctaggaaat cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt
    31561 tgtacgagtg ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg
    31621 tttaactgtt cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct
    31681 tttgcaacag cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt
    31741 aataaagtac catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata
    31801 ttttaattta tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt
    31861 tcgtcaaata aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa
    31921 aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat
    31981 aattctatca aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt
    32041 tctgggcgat aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca
    32101 ggattatatc cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg
    32161 ttgccagaaa aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca
    32221 tttaaaatag gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca
    32281 tcaagtttga ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga
    32341 gaatggagtt caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga
    32401 tgctccaaaa ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt
    32461 tgatatcggc taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc
    32521 ccgccttcat aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct
    32581 gttccagtga aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta
    32641 gaaatcattt ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt
    32701 tcaggaaaac cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg
    32761 atgatctcta cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag
    32821 taaggaccat gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga
    32881 tcatcccagc ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag
    32941 attcgtcttg aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg
    33001 tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc
    33061 gaattgatca aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca
    33121 cgaaaaactt tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc
    33181 ctttatgtta ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat
    33241 ttcttttttt agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa
    33301 agcttttgca aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc
    33361 aagtgttgcc attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt
    33421 taaagcagat tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac
    33481 acttaagtac atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa
    33541 ttttttgcca tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa
    33601 tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc
    33661 gtcatctatg gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat
    33721 tccttcatct gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc
    33781 atctttcagt gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa
    33841 gatgactgtg cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata
    33901 ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg
    33961 atctagtcga atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac
    34021 aatattatac tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac
    34081 aagttggttg acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa
    34141 tgaataatgg aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa
    34201 cagcaagggg aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc
    34261 gatacgctgt acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta
    34321 acgatcaaat tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg
    34381 cgtttgaaat ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg
    34441 atccagattg ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagca
    34501 gggaaggttc gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat
    34561 ttgaccttgg attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt
    34621 tgtatgataa ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa
    34681 ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt
    34741 ggcgatgtca tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca
    34801 gacagtgaaa ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc
    34861 tttatctcat taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc
    34921 gatgaagaag taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa
    34981 cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat
    35041 gacccagaag atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat
    35101 gatgaagatg aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac
    35161 ccgacagatg atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac
    35221 gaagacgatg atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa
    35281 acattatttt cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg
    35341 tgacacgatt tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa
    35401 aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg
    35461 agtggagaat gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc
    35521 tgctgaatgg actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg
    35581 tacatgataa agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact
    35641 attttgaaaa agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc
    35701 gcacggcatt agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc
    35761 aagatatccc ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag
    35821 ataaaataga cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt
    35881 tgaaagaaat tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc
    35941 gctcattgaa tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc
    36001 caaaaagaaa atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg
    36061 cttaaagagg agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag
    36121 cggagaaaga gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca
    36181 agtagccttc tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga
    36241 tcagtcattg aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt
    36301 caggtaccga agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat
    36361 caaaaatact ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat
    36421 tttgaaacgg atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg
    36481 tttctcgtga ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga
    36541 tacgacacaa tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat
    36601 gacacctatc tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag
    36661 gaagatccat ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa
    36721 ccaacacttg ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg
    36781 actttttctc aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct
    36841 aagtccgtga caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt
    36901 agttgcgact agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa
    36961 gagaaagaag agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg
    37021 tgcttttgtt tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa
    37081 cacactcttc ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa
    37141 gccaatcaac tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac
    37201 aaagtcagtc atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca
    37261 aaagcgtcgc aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc
    37321 gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc
    37381 agcatcctga aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat
    37441 acgatcatca gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag
    37501 cagacactga gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg
    37561 gttctgtcac gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg
    37621 attcagaaga ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag
    37681 tatacgatgg ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc
    37741 ctcaagtgat cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat
    37801 tagggcatcc aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt
    37861 aagtgagaca agtatcttta ataaataaca cattttaata agtagttgaa cacataacta
    37921 ccttacttgc aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt
    37981 ttctttggcg gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa
    38041 aatagcagta gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac
    38101 gtgatatcca ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa
    38161 attacaaggg gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag
    38221 cagacacaga aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat
    38281 aacagtgagg agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa
    38341 gttgttgagc cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga
    38401 gaagagacaa attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg
    38461 atcgaacaag tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt
    38521 gtagaataca tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag
    38581 attggagcta cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca
    38641 gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat
    38701 tatgcgccgg aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga
    38761 aaatggaaag aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc
    38821 gactatttac aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg
    38881 tacattattc ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca
    38941 aaccgaaaaa atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa
    39001 caagcagata aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg
    39061 aagaaaacga tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc
    39121 cggcaaaaag ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa
    39181 gttgcaactg agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat
    39241 ttggaaacag acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg
    39301 aatagtacag aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa
    39361 ttagtcgatc aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg
    39421 aaaaaagatg agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga
    39481 atcccgagtt catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa
    39541 gtatttcact ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca
    39601 gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt
    39661 atcgtatatg ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc
    39721 tatgctggat gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca
    39781 agaacgctat aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt
    39841 atcggaaagt atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa
    39901 tctgaatttg atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt
    39961 ttatcaaagc cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca
    40021 gttggcagaa tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca
    40081 gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc
    40141 agaaggcgag aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca
    40201 attgtctaat ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat
    40261 tggtgaagcg ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa
    40321 cggtaaccac cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt
    40381 actcaatgag ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat
    40441 tcatagtatt gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt
    40501 agtcatgctg aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa
    40561 aggtgaagtc gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt
    40621 cccgatcatc atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat
    40681 gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt
    40741 aaaaggggta accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa
    40801 tgacagttaa agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta
    40861 tccaagatgt agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt
    40921 tggcacactt gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc
    40981 atgaaggttc attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag
    41041 aagaattacc atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca
    41101 cttctaaatc agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg
    41161 ctcctggcgg aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg
    41221 gtaaagaaac agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta
    41281 aaacattgaa cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca
    41341 caggtgacca aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg
    41401 ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa
    41461 ttcctgaatt gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt
    41521 cattaacaga attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag
    41581 caatgaaaga agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag
    41641 atatcgttgg tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag
    41701 ttggcgacaa acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg
    41761 cacaattagt acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt
    41821 gtaaattata gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt
    41881 tgataataag tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat
    41941 ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg
    42001 atcactgacg acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa
    42061 ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa
    42121 ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca
    42181 ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc
    42241 gacgttgtcg tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa
    42301 aatgatacag aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct
    42361 ttcggtacag ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca
    42421 acagttgctg gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa
    42481 ccaaaacgac caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta
    42541 atcgaaaact tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca
    42601 ttctacgaag caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa
    42661 ctagcaaaag aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc
    42721 tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat
    42781 ggtttgatgg ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa
    42841 ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct
    42901 aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc
    42961 ggtggcggag actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac
    43021 atctcaacag gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta
    43081 gcagcaatca acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgaacaa
    43141 aaggtgaagg gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc
    43201 ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc
    43261 aaaagaattt gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt
    43321 catcggatct cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact
    43381 aaaaatctca gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc
    43441 accagcagct cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg
    43501 tgaatatttc catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa
    43561 tggtatgaca ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac
    43621 tgctgaatgg atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaggt
    43681 gtcaaacatg gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga
    43741 cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg
    43801 aaaagaagta tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat
    43861 tgctgaatac atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca
    43921 agcagattca ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa
    43981 atgcaagaaa tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc
    44041 cttaggaaaa aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg
    44101 ctcgcgagat cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa
    44161 gcggagcttt tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag
    44221 ttgaattacg tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg
    44281 acaacgtaaa caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg
    44341 ctatcgacaa agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga
    44401 acgctatttt aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac
    44461 ctttatacca ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca
    44521 tcatcaacgg cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc
    44581 ctgttggcgc tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat
    44641 tagcttcaat cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg
    44701 ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag
    44761 ctggctatgt acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct
    44821 acgacaaaga aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg
    44881 acgaaatgat caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag
    44941 acggattaga cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca
    45001 aagttcaatt agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta
    45061 tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag
    45121 aaactttcga agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc
    45181 gttctggtga aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc
    45241 aaatcaaaac tggttctcta tcacgtactg accgtattgc taaatacaac caattactac
    45301 gtatcgaaga ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact
    45361 taaaaaacaa ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga
    45421 gtatgatcac ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga
    45481 gtttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
    45541 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
    45601 agtagaatag gaatctatga gcaagtcctc tcgtataaat aaaataggag ggaagtagca
    45661 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
    45721 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
    45781 agaagaaaaa atcgcgattt tacaagagat catccgaatt aaatcagtga atggaaacga
    45841 aggtgaagta gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat
    45901 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
    45961 aaaagtgtta ggcctatctg ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg
    46021 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
    46081 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
    46141 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
    46201 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
    46261 agagccaacc aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
    46321 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
    46381 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
    46441 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
    46501 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
    46561 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
    46621 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
    46681 tcctttgatt cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc
    46741 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
    46801 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
    46861 tttggatatg atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
    46921 taataaatga aaaaacagag ccagattagc attattt
//
LOCUS       DAZKAY010000010        44884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000010 DAZKAY010000000
VERSION     DAZKAY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.010"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8WP_000524"
     CDS             212..604
                     /locus_tag="ACG8WP_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9026861.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8WP_000525"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8WP_000525"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9026862.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8WP_000526"
     CDS             2698..2943
                     /locus_tag="ACG8WP_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026863.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8WP_000527"
     CDS             3165..4775
                     /locus_tag="ACG8WP_000527"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9026864.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8WP_000528"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8WP_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026865.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8WP_000529"
     CDS             5067..5936
                     /locus_tag="ACG8WP_000529"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9026866.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8WP_000530"
     CDS             6101..7372
                     /locus_tag="ACG8WP_000530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9026867.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8WP_000531"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8WP_000531"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9026868.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8WP_000532"
     CDS             8763..9026
                     /locus_tag="ACG8WP_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9026869.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8WP_000533"
     CDS             9471..9920
                     /locus_tag="ACG8WP_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9026870.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8WP_000534"
     CDS             9977..11395
                     /locus_tag="ACG8WP_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9026871.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8WP_000535"
     CDS             11395..12255
                     /locus_tag="ACG8WP_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9026872.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8WP_000536"
     CDS             12429..13202
                     /locus_tag="ACG8WP_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9026873.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8WP_000537"
     CDS             13349..13714
                     /locus_tag="ACG8WP_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9026874.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8WP_000538"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8WP_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9026875.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8WP_000539"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8WP_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026876.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8WP_000540"
     CDS             15560..16216
                     /locus_tag="ACG8WP_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9026877.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8WP_000541"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8WP_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9026878.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8WP_000542"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8WP_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9026879.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8WP_000543"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8WP_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9026880.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8WP_000544"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8WP_000544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9026881.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8WP_000545"
     CDS             21888..22202
                     /locus_tag="ACG8WP_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9026882.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8WP_000546"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8WP_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9026883.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8WP_000547"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8WP_000547"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9026884.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8WP_000548"
     CDS             23546..23875
                     /locus_tag="ACG8WP_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9026885.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8WP_000549"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8WP_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9026886.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8WP_000550"
     CDS             24863..25687
                     /locus_tag="ACG8WP_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9026887.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8WP_000551"
     CDS             25680..26027
                     /locus_tag="ACG8WP_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9026888.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8WP_000552"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8WP_000552"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9026889.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8WP_000553"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8WP_000553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9026890.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8WP_000554"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8WP_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9026891.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8WP_000555"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8WP_000555"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9026892.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8WP_000556"
     CDS             31684..32451
                     /locus_tag="ACG8WP_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026893.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFIVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8WP_000557"
     CDS             32441..34483
                     /locus_tag="ACG8WP_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026894.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8WP_000558"
     CDS             34764..34913
                     /locus_tag="ACG8WP_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9026895.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8WP_000559"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8WP_000559"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9026896.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8WP_000560"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8WP_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9026897.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8WP_000561"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8WP_000561"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9026898.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8WP_000562"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8WP_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9026899.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8WP_000563"
     CDS             39330..40412
                     /locus_tag="ACG8WP_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9026900.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8WP_000564"
     CDS             40412..41215
                     /locus_tag="ACG8WP_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9026901.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8WP_000565"
     CDS             41285..42025
                     /locus_tag="ACG8WP_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9026902.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8WP_000566"
     CDS             42036..43040
                     /locus_tag="ACG8WP_000566"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9026903.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44884)
                     /locus_tag="ACG8WP_000567"
     CDS             complement(43085..>44884)
                     /locus_tag="ACG8WP_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026904.1"
                     /translation="LRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVF
                     YTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEK
                     ILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPL
                     ISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAV
                     SEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIG
                     VLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAII
                     HCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKND
                     LLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEML
                     CSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRE
                     THAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMK
                     DVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14427 a         7831 c         9345 g        13281 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgagggtga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc atagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaa
//
LOCUS       DAZKAY010000011        41189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000011 DAZKAY010000000
VERSION     DAZKAY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.011"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1046)
                     /locus_tag="ACG8WP_000568"
     CDS             complement(66..1046)
                     /locus_tag="ACG8WP_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9026905.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1059..1937)
                     /locus_tag="ACG8WP_000569"
     CDS             complement(1059..1937)
                     /locus_tag="ACG8WP_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFP9026906.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(1953..2753)
                     /locus_tag="ACG8WP_000570"
     CDS             complement(1953..2753)
                     /locus_tag="ACG8WP_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9026907.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3028..3177)
                     /locus_tag="ACG8WP_000571"
     CDS             complement(3028..3177)
                     /locus_tag="ACG8WP_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026908.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4145..4471
                     /locus_tag="ACG8WP_000572"
     CDS             4145..4471
                     /locus_tag="ACG8WP_000572"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9026909.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            4479..5387
                     /locus_tag="ACG8WP_000573"
     CDS             4479..5387
                     /locus_tag="ACG8WP_000573"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9026910.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6013..6267
                     /locus_tag="ACG8WP_000574"
     CDS             6013..6267
                     /locus_tag="ACG8WP_000574"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFP9026911.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            6809..6967
                     /locus_tag="ACG8WP_000575"
     CDS             6809..6967
                     /locus_tag="ACG8WP_000575"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9026912.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7039..8264
                     /locus_tag="ACG8WP_000576"
                     /pseudo
     CDS             7039..8264
                     /locus_tag="ACG8WP_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(8353..8745)
                     /gene="rpsI"
                     /locus_tag="ACG8WP_000577"
     CDS             complement(8353..8745)
                     /gene="rpsI"
                     /locus_tag="ACG8WP_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9026913.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8761..9189)
                     /gene="rplM"
                     /locus_tag="ACG8WP_000578"
     CDS             complement(8761..9189)
                     /gene="rplM"
                     /locus_tag="ACG8WP_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9026914.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9723..10190)
                     /locus_tag="ACG8WP_000579"
     CDS             complement(9723..10190)
                     /locus_tag="ACG8WP_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9026915.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10310..11848)
                     /locus_tag="ACG8WP_000580"
     CDS             complement(10310..11848)
                     /locus_tag="ACG8WP_000580"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9026916.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11928..12614
                     /locus_tag="ACG8WP_000581"
     CDS             11928..12614
                     /locus_tag="ACG8WP_000581"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9026917.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12815..13342)
                     /gene="msrA"
                     /locus_tag="ACG8WP_000582"
     CDS             complement(12815..13342)
                     /gene="msrA"
                     /locus_tag="ACG8WP_000582"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9026918.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13426..14103)
                     /gene="pgmB"
                     /locus_tag="ACG8WP_000583"
     CDS             complement(13426..14103)
                     /gene="pgmB"
                     /locus_tag="ACG8WP_000583"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9026919.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14100..15228)
                     /locus_tag="ACG8WP_000584"
                     /pseudo
     CDS             complement(14100..15228)
                     /locus_tag="ACG8WP_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(15228..17411)
                     /locus_tag="ACG8WP_000585"
     CDS             complement(15228..17411)
                     /locus_tag="ACG8WP_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9026920.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17408..18433)
                     /locus_tag="ACG8WP_000586"
     CDS             complement(17408..18433)
                     /locus_tag="ACG8WP_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9026921.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18478..19305)
                     /locus_tag="ACG8WP_000587"
     CDS             complement(18478..19305)
                     /locus_tag="ACG8WP_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9026922.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19302..20369)
                     /locus_tag="ACG8WP_000588"
     CDS             complement(19302..20369)
                     /locus_tag="ACG8WP_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9026923.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20395..21231)
                     /locus_tag="ACG8WP_000589"
     CDS             complement(20395..21231)
                     /locus_tag="ACG8WP_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026924.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21228..22097)
                     /locus_tag="ACG8WP_000590"
     CDS             complement(21228..22097)
                     /locus_tag="ACG8WP_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026925.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22167..23450)
                     /locus_tag="ACG8WP_000591"
     CDS             complement(22167..23450)
                     /locus_tag="ACG8WP_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9026926.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23464..25149)
                     /locus_tag="ACG8WP_000592"
     CDS             complement(23464..25149)
                     /locus_tag="ACG8WP_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9026927.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25424..26449
                     /locus_tag="ACG8WP_000593"
     CDS             25424..26449
                     /locus_tag="ACG8WP_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026928.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(27044..30697)
                     /gene="rpoC"
                     /locus_tag="ACG8WP_000594"
     CDS             complement(27044..30697)
                     /gene="rpoC"
                     /locus_tag="ACG8WP_000594"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9026929.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30776..34393)
                     /gene="rpoB"
                     /locus_tag="ACG8WP_000595"
     CDS             complement(30776..34393)
                     /gene="rpoB"
                     /locus_tag="ACG8WP_000595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9026930.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            35121..36089
                     /locus_tag="ACG8WP_000596"
     CDS             35121..36089
                     /locus_tag="ACG8WP_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9026931.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            36107..37012
                     /locus_tag="ACG8WP_000597"
     CDS             36107..37012
                     /locus_tag="ACG8WP_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9026932.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            37009..37815
                     /locus_tag="ACG8WP_000598"
     CDS             37009..37815
                     /locus_tag="ACG8WP_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026933.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37972..38940
                     /locus_tag="ACG8WP_000599"
     CDS             37972..38940
                     /locus_tag="ACG8WP_000599"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9026934.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39257..39781)
                     /locus_tag="ACG8WP_000600"
     CDS             complement(39257..39781)
                     /locus_tag="ACG8WP_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9026935.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            40068..>41189
                     /gene="scm"
                     /locus_tag="ACG8WP_000601"
     CDS             40068..>41189
                     /gene="scm"
                     /locus_tag="ACG8WP_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9026936.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTS"
BASE COUNT        11886 a         8675 c         7588 g        13040 t
ORIGIN      
        1 ttacaaactt ctttgttttc tttttttaaa cgcaataata aaggttttaa tttcagcctt
       61 tttaattaga aaatcaattt tcccaaatta ttttctttag ctttcttaac gatttctgtc
      121 gcaactacca catctaatac agccgaacca actgttttga agatcgtgat ttgttctcca
      181 cttgtccgac cgatcaattc ttcattgatc agttggccta gttccccatg atagctgtct
      241 ctttgaatat acccatcttg cagcggtgaa atgaagtccc cagcttcttt tagaacaccg
      301 tccatggtat caaaaataac aacatctgcg gctttgatta tttctcgtgg aatttcacac
      361 atttccggtg tgtaagcacc gactccattg atgtgcgcac cttttttgac ccactcagca
      421 gagaatgtcg cacgttttga agtggtcaca cttgtaatga tgtctgctcc ttcaacgcat
      481 tcttgactgg tttttgttgg gcgcatcgtt acagaaaatt gttgcatcat tttttcagca
      541 aattggctgg cacgctcaaa atcaatatca aaaatccgaa cttcttctaa tttacgaacg
      601 gtcaacattg cttctaattg tgactgtgct tgtccgccgg ttccaatcag cgcgccaatt
      661 ttagcatctt ctcttgcaag aagttctgtt gctgctcctt gaacagcgcc tgttcttaat
      721 tgtgtcaaat aagtaccatc caaacaagcg gataccatac ccgtttctgg atccaatacg
      781 atcatcgttg ctggtacact tggtaagttt ttcttgatgt tttcaggata cacggaaacg
      841 attttgacac ctagcgcgcc ttcgccacct tctacataag caggcatata taagctttgt
      901 ccgttgcttt ttggcacatc gatattggtg cgaagaggca cacttgcctt tcctgctgaa
      961 tacaatgaca gtgctttttt gtccgcatcg atggcttctc tcattgaaaa acttttgata
     1021 atatcttctt ttttaagaaa taacatttga atcgctcctt aattagctgc tgtaactttt
     1081 tttaattgac tgcccaacat cgtatatgca ctgtatagta agaccaaaat cacgatccat
     1141 ttcaggatat aggtatctaa agatttgact aggaatacag cgaccaatac ccccaataca
     1201 ccgaaggttg aagtaaaaag tgtgattttt ctgctgtatt cattaaattt aatgaattga
     1261 acactgccga tcggtactga gaaggtacaa gcccccatca taataggaaa ggctgcaact
     1321 ggattcaagc ctagcaagta gacagttacc atcgttaacg cataagaacc aatgccgata
     1381 ttgttcaatg caccatagac aaagagtaaa aatcctgcca agattaattt tccgccatac
     1441 aattcactgg ctgttccgtt tgatggaatc cagttcattt gtcctgcaaa aatcaacaat
     1501 gcagcaatga ccaatcctat gccgacaaac agtttgatcg ttttctctgg taacttcacg
     1561 acaaatcttg gtcctaaata tgcaccgata atttggcaga taatacaaac agctagtgtc
     1621 ttgatcccta cgttgatggc agtgatgtaa gacaatgcca tgacggctac cggtactaca
     1681 cattgggtat tcaacgttcc gggtagtttt ttcatgctga cccatttcaa ctttggataa
     1741 aggacggttc cgatagcaaa gtcggaaacg ccaaatgttg aaagaaagaa gatgataaat
     1801 gacgtgaagg gcaacagacg catatcccct ggttcttctt tgaaattttg tttgtttttg
     1861 aaaaggtctc ttagaaagta aataataaaa tatagattga ttgcgacaat tagtgtcagc
     1921 aaaatattcc gaatcataaa aaattcctcc tactagtaaa tctttctgat tccctcaaag
     1981 ccctcaccat attttgcttt aagttcttca ggagattctg caatcacttt tagtgtatat
     2041 gcatcatgtt tgacatattc ttttgctaga ttgaccatgg cgtggttttt tgtccaagtg
     2101 acatcggttg attgcagttc ttcgttaatg gtaccaaaca cgacttcttg ctcatcggcg
     2161 acgatgttga tccatcttgc tccaaagtca gcaagtttgt ctgtttcaaa accatgaata
     2221 taatactttt ctaagggaag atcatattca tgggtagaac tgaataaaat cagtacaaac
     2281 ttatcaactc ttttttcagc ttgctgcaat gcctttagta gtgaatccgt gaaattctca
     2341 tgccaaactt gaatgagcaa actttctttt gcgtttttca ccaaatgctc tactttatcc
     2401 agaacatact cgataccatc aactttccac agcatttctt cttcatcttt ttctttgatc
     2461 atccctagat tcttggtaag aaaagaaaga tcatttttaa cacttttttc taatttctct
     2521 aaaaactcat ttgccgagat ggcatgataa agtttaggtt ctgatttatt aacgacaatc
     2581 aaattctttt ttaatagttt ttccaaaata ttatatactt tcgagcgtgg cacacctgag
     2641 attttactta cttcatatcc tgtaagatta cctttttcta atagtgtggt atacactctt
     2701 gtctccatct ctgaaaaatc atattttttc atgatttgaa taatcgtttc cattgattaa
     2761 tcctcacttt atttttagta gctactacgg taacaactat aaattctttt tgattatctg
     2821 tcaagagtta gttcaataac actcatataa taattagctt aaaaacaatc tttttattaa
     2881 aaagaaacat tataatatga atgtttgtga aaaaataata caacttagtc attctttaca
     2941 acgatttata gaaaaacaac ctctttaaat ataaaaggta cgggttactg attacttgta
     3001 aatctttacg ttccagtttt attctttcta aaaaatatac tcacttcttt tcttagactt
     3061 ctttttttga ttctcctctt tatggtcttg tccttttttg ccttcaaaat atttaaataa
     3121 acccctttca aaagcatttc taggttttgg acaataagga atgacttttt tcttcatata
     3181 aatacctcat ttaactattt gatatcatca cattatcaaa aaaaataggt tttttattaa
     3241 aactatcttg acctttcggg agatttcact gtgatttatt tttattccca tacttttttc
     3301 gttggtcact ttcgtatacc taatctaagc gatcatcggc gacggtgctt ttacaacgaa
     3361 tggcaggaca aaacaagggg cggaaaaact tttttgccta tttcataaaa aactttttcc
     3421 tacagcaatc ccttgttttg tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg
     3481 cttagctata cgaaaatgac ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga
     3541 aattgaaatc tcccaaaatg tcgtccgcct cggagacaac ttgctgctga aaatggttgg
     3601 ttaattcatt taagtattaa ataagataaa tcatcaaatt aatcggaaaa atagcatgat
     3661 tggccttatc aaaaataaac tgtaaaaact tatatttcta tgagtggtgg aacaatgcta
     3721 ataccaaatt aatttatcga ttacccacct gcacaaacca aagatagcat tattaggaaa
     3781 cgaatcaatc agcgaatcca gcattataat gaggatacta aaaataaagt tttcataatt
     3841 ataaatccgc cttcctacga aaggcaccaa tgaggtgatg aaaaccttag agcgctttct
     3901 ttcttttttt attccgtgtt accctttcct tgcaggtccc tggtacacta cagaacccga
     3961 ggaggtgagt ggcggggctg tatagcggca accccgcata attatatggt gtcggtcatc
     4021 cgttaaggca tcaatgaatg gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt
     4081 gactcgattt cgccggatga ccagtcccta ccaaacctgt actatttttt tacagaaggt
     4141 tgctatgttc aaaatctttc gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat
     4201 ctatgcgtgt atcggaatca cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt
     4261 ctcttccttt acaaaaggtc tcaatgaatt agctgaatgg cttgctaaat acaactgtaa
     4321 ggatgtgtgt ttggaatctt ccggtaaatt ttggattcct gtttttaata ttcttgaaaa
     4381 aacttgtttt gtcacactgg ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac
     4441 agatcgcaaa gatgcaaaaa tggatttgtg atctctttat gtgcgacatg atcaaaccca
     4501 gttttattcc tggtcttgaa atccgccagc ttcgtgatct aatgcgttat cggacgaaac
     4561 tgaccaacat gaaaacaagt gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc
     4621 tgaaactaga tgatgttttt agtgatgtgt tcggaaaatc atccacttct atcatccaat
     4681 acttgttaga acatcccggt gaatcatttg atgttcgacc atttatccat gcccgttgta
     4741 aaactcccgt tgaagaaatc caagcggcag ttgagggggc gatttctgta cctcaagcga
     4801 ttaagcttcg ccaatgcctc aaccatatta acgagattga gaagcatcga gaagagatca
     4861 atctagagat tcaagctctt gcagaaccct tctcccctgt tttggaacta cttcgtacca
     4921 taccgggctt agacactcag ccaataacag cgattgcgat tctttcagaa ataggaccgg
     4981 atatgtccgt gtttccaact tcgaaacact ttatttcttg ggctggatgc tgtccaagaa
     5041 acgatcgcag tgcccgacga acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga
     5101 aaccgctact tgtacagatt gccaatgctc ttctcagatc caaagaacat ccagaattta
     5161 aaaatcgcta tcgtagaatc aaagcgaatc gtggacacaa gaaagctatc attgctattt
     5221 gtaagatgct cctgaccgct atctggaatg tcttatcaaa ggtggaaccc tattctgcta
     5281 aaggatatct ggagccaaaa cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc
     5341 tggacctttt aagaaaacgc ggatatacca ttatagatag tgcttaaacc aaagttacgt
     5401 tgattcttcc taaatagact cgttgtcttc catttcaaaa attttgaaaa cggtaggctt
     5461 atttagtgta ccgttcttca ggtatcttca aatagattta ttttcaaact tatttccttt
     5521 cttcttttac tctcctatag cttagcattt aagtgggcac tcgtgtcacc ttgctagata
     5581 gctatgaatg ccgtaataag cctcctagta tataggcaga ctgccaatta taactgattc
     5641 aatccacatt atgataaaat ccaccttttc gtttcatatg tgttgaaata tcacatatgt
     5701 caaaagtgca cgagtctttt cattcagtgc tttgtaaata ctcaaaatta ttccttttca
     5761 ctttaaaaaa aagataatta gtacaaaatc agaaatgagg aaagtattag gatggactag
     5821 aaaggagttg ttgaatttgt tgcaacagat tggtcattta tcaattaaaa attaggtgaa
     5881 gttttattca tcactgcagc tgctgtttcc cagagaataa aacgtctaga agaaactggt
     5941 cagttactgt attatcaagc agcaattgac tacgaacaag cttgattact aaccaaagcg
     6001 tttatccacc tgttgattaa atcaaatcaa aaatcaggtt tttatacgtt cataaaagca
     6061 atctccagtg tactcgaatg tgactgtatc ataggtcctt attcgatgtt aatcaaaatg
     6121 gggtttcctt ctaccgaatc attggacagt tttatcaatg atttatcaaa gtttggacga
     6181 accaacaccc aaatcgtttt ctcgactctt gtttccttaa tggtttatca gttctggaat
     6241 gtttatcatg cgcttttatt aaaatagaaa agagcttttt tcatgcgaca aagcagtgat
     6301 catttattgg cacatgcaat ctttgtggat ccattttttg aaaatacgat atttagtttt
     6361 gacccaaaaa tctttaaata cgtatttgat aatttatctt aacaaccaga taattttatt
     6421 cagcaccgta ttatctatat tgaattctcg gaatcaagat aaattttttt ccgtttttgt
     6481 ttctttttta atcttcttct tgttattaat agtgattagg atgatattcc tatattgatc
     6541 tttgataatt agatataatt tttgaataac gttccctgct attcgagcta gaaatacaag
     6601 tacgccatga cctagttagc gacaactact tagagatttc ttttgttctg ggacaacaaa
     6661 atgccatgac aatagcagta aataatgata cttccgtaag gctcgccgta aactggggag
     6721 aggtgaaatt cctatgtgct tacccaaagc ggttcaaaaa ttttaaacta ttattttagg
     6781 agatgatttt ataaaatcga aagcctactt gaataaaaaa caagcctgtg aatttctagg
     6841 aattgataat aatactttag ataagtggat tcgtgtatat aattttccag taataaaaat
     6901 tgatggcgta gttcgctttg acgtagagga tatcaaagat ttcatgaata cattcaaaaa
     6961 aaattgaaat tttcttctcg tcacatatac tataaatagc gaaatgtact gccttacttt
     7021 ttaaaggagt gaggaagttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg
     7081 aggtatccaa aaaaattcag agaaatcact ggaattgcat cagagaagta tcaaaaagta
     7141 tttccaacta ggcaattagc tattaaggcc gaaagggaaa tgaaaaagaa gattgaaaca
     7201 gttttaagag aggagaatgc aaactcacta gaattgaaag gaaaaataaa atttaaagat
     7261 ttttatgaat caaaatggct tcctagatat gaactaggtc aaactactag gtcaaacagg
     7321 gctccaagta atgtaacaat tagtaatact aaggatatat tccgattgca catccttcca
     7381 atgtttggtg aatatgcaat gaattatctg aattcaaata ctgagataat atcagatgaa
     7441 ttaacaaaga aatcgaaaga atacgcaaat ataaaaatta tcaaaggcta tgttcgctct
     7501 atgtttgata ttgccgaaat actatgttat atcgaattca accgaactac caaaattatt
     7561 caatgtatta ccgcgcctaa gaaaatggct ttagaagaaa aaaggataag ggaagggaat
     7621 caagcattat catcagaaga gctgactgat tggatagaag cagtaaacaa tgacttaaat
     7681 aatcacttgc ttacacttca cgattacaca cttttcatgc ttaccttata tctaggtgat
     7741 agaaaaagtg agacttatgc tctgcaatgg aaatatattg attttaataa aaatacggtt
     7801 cgtctcaaat atgctttaga taaatatcaa agaaagacac atacaaaagg ttggaaagac
     7861 accgttattc aggcaccaga agttgtgatg acattattaa gagaatggaa atcagctcag
     7921 gctgaacaat tgttgaaact aaaaattata caaacacctg accagtacct ctttacctat
     7981 tccaaacctt ctggagaagt aaattgtcct gttcacgctg actacttaaa ctatcgaatc
     8041 aatgcaataa aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata
     8101 cctatgcaac tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa
     8161 cgcactcgga tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat
     8221 ctgtttttga agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg
     8281 tgttcttatt ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat
     8341 ataaaaagaa tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg
     8401 ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa
     8461 acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga
     8521 ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc
     8581 agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc
     8641 aacatctttt ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac
     8701 tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta
     8761 ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc
     8821 tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat
     8881 accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt
     8941 gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt
     9001 tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa
     9061 atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag
     9121 acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg
     9181 tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca
     9241 tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg
     9301 ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact
     9361 tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc
     9421 tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc
     9481 ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt
     9541 gtgactcgga gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga
     9601 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc
     9661 taatactcag gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt
     9721 ttttacgcgt cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt
     9781 tctgtggctg ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca
     9841 gaaacttcga ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt
     9901 tccaaacgtt ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta
     9961 tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat
    10021 acttcatcta actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta
    10081 aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt
    10141 agatctgcaa ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc
    10201 tcctaacatt ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat
    10261 catttacctg agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag
    10321 aatcagctaa ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt
    10381 gtgtcgaaac aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc
    10441 caacttgctc tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga
    10501 tgcttctcca gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa
    10561 caagcgggta acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc
    10621 cgcctgtagc acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat
    10681 aaaggttgag acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc
    10741 gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa
    10801 gtaaataggg atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa
    10861 gcagtgcatc atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa
    10921 atgtatgccc tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc
    10981 aatgtttctg atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag
    11041 tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa
    11101 aagcacctaa ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat
    11161 catttggcaa acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg
    11221 aaggaaagac ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg
    11281 cccattggaa ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga
    11341 tcagttcgcc aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt
    11401 ttttgaataa ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg
    11461 gatgaatcgg cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt
    11521 tttctgcttc gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt
    11581 tctcttcatc caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga
    11641 ttgtgtctga aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga
    11701 tcaattcctg atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat
    11761 aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc
    11821 cgatatctaa tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa
    11881 atttagttta agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt
    11941 ttatcattgg ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag
    12001 gtcactctat tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt
    12061 atgaattgat tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt
    12121 gtcggttgcc cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt
    12181 tctttacttt ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc
    12241 tattttcgtt gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt
    12301 gggcaatttt atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt
    12361 taacgtttat cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg
    12421 atactctgtt gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct
    12481 tgtttcttgc tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa
    12541 aaaagttgga aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc
    12601 atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg
    12661 ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa
    12721 atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga
    12781 tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac
    12841 gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct
    12901 tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg
    12961 tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa
    13021 tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag
    13081 cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat
    13141 cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc
    13201 ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca
    13261 cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg
    13321 cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc
    13381 aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac
    13441 ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac
    13501 atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc
    13561 caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac
    13621 attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg
    13681 cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg
    13741 tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag
    13801 aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata
    13861 tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat
    13921 ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc
    13981 gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta
    14041 atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt
    14101 catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg
    14161 cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg
    14221 atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt
    14281 ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct
    14341 tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc
    14401 tgtacccgta tcgaactcca gtaaatccaa tacgtagtcg atgccagaga ctattttccc
    14461 tgaagcaaat gctaacagtc ctgcacccat tccaccacca gcactactgc ccgatgcctc
    14521 gattacttga ggggcaaatc tcatataaaa atcatgcatg gcttggtcag ccactgctaa
    14581 ttcattttct ttcaagcctt tttgtggtcc gaaaacaaaa gaagcgcctt cttttccgca
    14641 taaaagatta gtaacatcag taatgacagt gattttggct ttaatcagct caataggcac
    14701 gttcgtttta tcaactgtag caattttacc tagattttct cccagtgctt ctactttttg
    14761 attatcttga tcgtaaaaag cataacctag accttctgcc atgccgatgc cgccatcgtt
    14821 ggtactactt ccgccaacgc cgatgatgat gtgtcgaaca ccttgttgca acaattgtcg
    14881 tatcagttct cctactcctc gcgtggataa cctaagtggc tgttgttggt ttagtgggac
    14941 atctttcaat ccgacgattt ctgccatttc aaaaatcgca gtttgaccgt ctgaagcgta
    15001 ctggatttct ttgtttttgc caaatgcatt agtaacgact gctgtttgcc gcttcaacct
    15061 taaactgtct gctaatgatg aaaccgttcc ttctcctcca tcgcctatag gtagcagatc
    15121 atattcagcc tgaacaaaaa cttcttgaaa acctgattga atggcttgtg ctgcctctaa
    15181 ggctgtcaga ctttctttga aagaatctgg cgcaatcaga atacgcatta ttttcccctc
    15241 cgttccaaga tcagttcttt ttccagctgg tattggtctc cattgacaaa aataggtaat
    15301 gtttctttcg tctctttttt tattactatt tgtttttttg tcaaaacaaa tagcaacctg
    15361 tgatttttcc aaataaaagg aaaagaaatt ttttcccatg ctgaaggaag tcttggagaa
    15421 atagaaagag ttccattcgt tactgtaata cctgaaaaac caaatataac tgctagccat
    15481 agtgctccta atgaagcagc atgaattccg tcatctgatg aatgcggatc tggtcctaaa
    15541 tcaatcaggc acgcttcttg gaataaatga tacgcccatt ccatttcttg gatacggcaa
    15601 gcctctatgg cgtgaatcgc tttactcaac gatgagtcgt ggattgtttt gttttcatag
    15661 taatgcaaat tcttagaaac gattggttga gaaaaatact caggtaataa gtataacagc
    15721 atgacaagat ctgcttgctt gctgacttgg agttcattta cttcgctacg ggcataatca
    15781 agtaaaatag actggctgcc aggacgttct ttgtaagtgg tcaaatcaat aactggctta
    15841 ctgagatacg tgtcatcttg cggaatcagc tgttctttgt taggcactgg caaatataac
    15901 ttatctaaaa aattttgaca ttctttcaaa aaaagcggat cgccagcttg atatttaaca
    15961 gctaaggcca cattataatg cgccagatag ttcgtatatg cattattgtc gatatattct
    16021 gtgtattcat ctggaccaat gactctgtgt atctctaaat gatcatcgac ttttacagct
    16081 ctgctcagcc aaaaagctgc tgtttcatga atgagcgttt ttccctttgt ttctaaaaac
    16141 gattgatcct ctgttgcttg gacatactct tgaacggcat aagcaaggtc tgcactgata
    16201 tgatgttctg cggcagctga agcaacttct tgtcttgttc cggttttgat attcaatgca
    16261 gcaaatttgg gtgtctcttc ctgaccagtg tgtgcgcttt cccatgggaa aagtgcacct
    16321 tgatagtgat aatctcttgc tttttgccgt gcgccttcta atcgatcata acggtacagc
    16381 agcaaattct ttgctacttc aggaaactga tataaaaaga aaggcaggat aaaaatttcc
    16441 gtatcccaga aaacatgccc tttataacct tcgccagtta gtcccttcgc ccctacacta
    16501 acacttggat cattgcgggg agtcataatc tgtaaatggt aagcagcgaa gtccatcgct
    16561 aactgatcga aagaagaatc gccttggatc gttatccgat gctgatgcca aaaatctgtc
    16621 caataagaca tcgactgctc taaaatttct gtataactaa cgcttttctg ggcttgggca
    16681 atacttttct taaacgggtc tgtttgctga agagaggtag agagcacgat gatcttctcc
    16741 agcgtaaagg acgtattttt tctgatcttt tctgaaaagg aagcggtgat ccggcgattt
    16801 tttgcttgga atacacctgc tttattgaat ttagctgcaa tcccaatatc aattttactt
    16861 tctgttgtct ggtaggatgc actgatcaaa tcgtcaccaa atactcgcag ttctttttca
    16921 accaactgct gtgtaccaaa attggtttgc tgtccatcga tacccgtttt cacatctact
    16981 tggcagtctt cggtcaacgg cttgatgacc actttcaggg ctgcctgatg gagattattt
    17041 tgatcagcaa cacgacaagt aatgacttcc actttttttc cttgagcagt ttcaatgagc
    17101 gcatagcgtt taagctctcc catttctaaa tctagtgccc gctcgtatgc aagaactttc
    17161 atcgtctcaa gagaaaaatg ttcaccgtcg atttttattt cccatctgat gacatctggg
    17221 agattcacta actcggctga ttctgctcct aaaggtcggt tatatacacc agctgcaaac
    17281 attcccctta cttgattccg atagtcttcc tcctggcttc ctctgatacc aaaaaatcca
    17341 tttccaatgg tcatctgact cgcatactta ttgatatact ctaaatcaaa ctgtttttct
    17401 tctactttca taaggtaata acttctcctg tttctgccga acgatataaa ttttccacca
    17461 gattctggat gacatatcct tgccagccat ctgcaatcat ctgagtctgg tcaccgagcg
    17521 cccgattcac aaaatgagcc atacttagtt catgtgggtc attaactggc gtggtctttt
    17581 gtaaggaaac cagctttcca tctacatctt cgaatagttc gcctggatac agattagcac
    17641 ctgcctgatc gccacaaaaa gccacattca gtttgtcttc tctgatattc aaagcaaacg
    17701 aagtattcag ttggatgatc ttgccgtttt ctaattccag tgtgccaaaa attgcatctt
    17761 ctactgtgaa tttgcttgga tcccatttcc cgaatttccc tgaatttttt ttcgtaccaa
    17821 ttttttggaa gctgtacgcc cgaacgcttt ttactttagg aaatccagta atatacatcg
    17881 ctgtatcaag catgtgaatg ccataatcga tcaaaggccc gcctccttga agttctttgt
    17941 cgataaacgt tccccagcct ggaacaccag accgacgaag cgcattcgct tctacgtaat
    18001 aaatatcccc gagaacatca agatgattct tgatcattgt tgcttcgtca gaaaaacgat
    18061 gctggaatcc ataagccaaa atctgcccgt ttttatcggc tgtctctgcc atctttcttg
    18121 cttgatcagc tgtcatagct ggtggtttct cgcacatcac tgaagcatta gcttcgaggg
    18181 cagccaatac atgttcgaag tgaaagcgat tggctgtaca gacactcacg atatcgggag
    18241 aaatctcatt cagcatttgt gtaactgatg tatacgcatg agaaaatcct gtacgtcggc
    18301 aaaagtcttc tgctcgctgg ctgactggat caaccacagc ggccaattct aagacatctg
    18361 atcttttttg ataataagca gcatgtactt tttctgctac ttgtccagcg cctatgattg
    18421 ctactttctt catttatttc tctctccttt gcttatttac ttattgttat tctttctcta
    18481 ttctttctct aaatcattta aatgacgcgc tgtttccaga cagtttttca agtattgcaa
    18541 tgattcctga taaattttca cttcattttc tcctcgcaaa cgacattcat ataccatata
    18601 accttcatac tcattttgaa gtagcgtttg aaaatggcga gtaaaatcaa ttgcgccaga
    18661 tcctggttgg tagcgatgat tatctgctaa atggatatga cccacatact ctttttgcgt
    18721 agtcaatgct tttgaaatat catcttcttc aatattcata tgataaaaat cagcgatgat
    18781 ctgcacatgt ttcaattgat tagagtcaat gtaatgacgc gcatccgcta acaaattgat
    18841 catatggtct tggtatcggt tcaaaggctc caaataaata accgtacctg tttcttcggc
    18901 tactttatct aaataacgaa gtgattcgct gactgcttta tagtctcctt ctttgcttcg
    18961 gggtgaaacc atcggcggta atcgataagt gaacattccc caagctgctg ggataacgat
    19021 tcctttgcct ccaacctcgc ttagtgcacg caaaatctca gcaatttgac gtacaccatt
    19081 aagacgccgt tcttcgataa agtcaccgat ccacccatcg tagccgttac aagctgtcac
    19141 tactgggatc cctgtttctt tgattgcctg tttgactgtg tcgatatttt ctattaacaa
    19201 cttaccgtcg atttcgtatt cttcaaatcc acagtctttg ataaatctaa atttttctaa
    19261 aatgtctctt gggaaaaatg cttgatcttg cgtaccgaat ttcattgttc tcctcctcgg
    19321 acttgaacgc ccattttgat actttcttct gggtgctggt ctacataacg catgtaagct
    19381 tctgcgcttt ctgcaaatgg cacaactgga tcaatgattt cttcacaatc caagtaaccg
    19441 ttcatcaata attcccaaca tgtgtcctcg attcgtttac ggttccatct tgggtaatct
    19501 gggtttggtt cactagatgc tcgagcaaag acgatttttg cattattgaa atgtgcttcg
    19561 cgaccaagat taaacccagc tggaaaaggt ttagcaaagg caacataaga aatgatcccg
    19621 ccatagccga tccctcttaa agaagcttgt aatgcggcag cactgccact cgtttcaata
    19681 actgaatcta ctccacgttt atctgtcaat tcctttagac gaaaaccgac atctgctttc
    19741 ataggatcta gcgtgtaatc ggcaccgtat ttttctgcta attctctacg ctgcgctatg
    19801 ggatcgatcc caacgatggt agtagcacct gcttttttag ctaattgaat cgcaatttgc
    19861 ccgatagcgc ctaaaccgaa gattgctaca gcatcacctg ctcgaacatg tgcttcccgg
    19921 ataccaccta aagcgaattg agcaggatcg taacatactg catttttcca ttgatccaac
    19981 cgatcaagtt tacgtagttt ataattatca acaccgtttg taatcagcgt ttcacgaatg
    20041 ggtccataag tgcagactaa ctctcctttt tgatactctg tcaccaaact gcccgtttct
    20101 tcgatcgtac cgacaatcat atttcctaat tgaaattcgc caaataccac accgcgttgg
    20161 cttccttctt ctcttggcaa gaataggcgc cattcttctg aaaattcttc atcgataaat
    20221 ggacttaatc ctctaaagtc gacaatttct gttcctggtt ttggagtagc gaaaattact
    20281 cggatacgta cttcgttctt ttcgatctca cggtcttcgt aatctttgaa tccggctatt
    20341 cttggttcta atgcaactaa ttttttcatc ttttctttcc tctttctttt ctttttatcc
    20401 ttttacgccg ccttcgacta aatcaccttt aataaatcgt tcagataacg catacataat
    20461 gatgactggt aaagcggtca ccaatgaagc ggccatcatt cgtccccaaa tgtagtctgg
    20521 tgtactgaat aatgtgttca aaccgattgg taaagtaaat ttttcggagg tcgacaggaa
    20581 aatcgaagca aataaataat cattccacga aaccataaag caataaacga agacagaaat
    20641 gattccagaa atagccaatg gtataatgat ccgaataatg atctggatcc ggctaagacc
    20701 atcgatcatt gctgcttctt caatatcagt cggaatcgtg tcaaaataac tttttagcat
    20761 gaatatcgca gtaggaagtg tttgaacgac catcgtgata atcagtgctg tattggtatc
    20821 atacaaacct agaccggaaa tgattttaaa taatggaaca acgatcaaaa taccggaaaa
    20881 catataaacg gtgtaaaagc tagcattgat tttcgagcga gctttgaagc gtagttttga
    20941 caaagcgtat gccccgctga tcccaatcac tactgcagtt acggaggcta tcaaggagat
    21001 cactaaacta tttttgaaat acatgatgaa tggaaaaatc tcagcattga aaatatcctg
    21061 ataatgttca aaagtccatt ctttcggaaa aatagtttgt gtcgttgcaa ttgcttcttg
    21121 tgaatccttg aatgatgtca tcagcatgat caaaaatgga aacaaattga cagctaatag
    21181 caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt ttatttttca tttcaagtcc
    21241 accttctttc gtgagagcaa gacgaccgct gcaataatga tgaacaatag aatcgatata
    21301 gctgctgctt ttccaagatc attgaacgca aaggctgtct gatacaaata aacgcctaag
    21361 atattgactt tattcgtcag tagatagaca tctgtaaaca tatagaacat ccagatagat
    21421 cgtaaaacaa tcacagttgc tagtactggt ttgattgccg gcagtgtcac gatcttgaac
    21481 ttctgccaag catttgcacc gtccatttca gctgcttcat aaagcgtttt gtcgactgtt
    21541 tgcaaaatag ccaaaaatga aataaacgca tacggatagt atctccagat agcaaacaaa
    21601 acgaccataa agaagctagc aactggctgg tcgaaccaca gcggtgcttc gttgaaaagc
    21661 tgcaacctat ctactaacag atcattgacg attccataac tattattgaa catatatttc
    21721 caagcaaaaa tcaaagaaat agatggcgta acataactta ataaaatcag tgaacgcacc
    21781 gttttcctaa atctgaactt gcggttaagc aaaatagcta cggctaatcc taaagctgta
    21841 ctgcctgcta caactaagaa tgtgtaagca actgttaaac ctaaagaacg ataaaattca
    21901 ctatcactta aaatttcaag ataattgctt aatccgataa aaacggcgtc tgagttagga
    21961 ttcagcggca tatcgaaaaa actgatctga atattggaaa gcattggaat cagcactaaa
    22021 acagctaata aaagcatgct tggtgctaaa agtcccatgg ctaattttaa gtcagaatgt
    22081 ttccttggac gattcattga aacctccccc tgataatctg ccaatagcta tctacttttt
    22141 tcgaacacca ttggcagaac ttcttgttat ttcgtatttt tttgtgcgtc agctaaagct
    22201 tgatcgacat cttcgtttcc gacagttact tggttcactg cttttccgat cacacctgag
    22261 cttgtgatat ctcccatttt cgtgaaattc ttttccccga ctagtccaaa gacttgtact
    22321 tcatcaaaag cactagcaat ctcagatggc agctcgccaa aagacttgat gacttcattt
    22381 tctttgtaac catccagttc gactacttgt ttattcactg gttgagcacc accaggagac
    22441 atcaagaccc acttttccat gtttttcggt tgagataaga aagctgtgaa tgcttcagca
    22501 gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca atccagatac tgtgccatag
    22561 acagcttgat ctttttgtgt tgggatagca aaaccgatat tttctgagtt ccccgcttca
    22621 taaacagaag gcaaaatgta agttgaatag attgccattg gtgctgttcc attcatgaaa
    22681 gcatctttga tttctgtcac atcattagag cctggcatcg tgtattggga taagttttga
    22741 taaaacgaca atgcttctct catcttttcc gtatcgatcg ttacctcgcc ttcatcatcc
    22801 aaaacatttg ctccatttga caaagcaaat tgagagaacg cttgctcact cattgttcct
    22861 tcagcagtag gcatagcaat tccatattgt ttattctctt tatctgtgaa atgttgagca
    22921 atttttaaaa tatcatccca attttctggt tctgaaaaac cagcttctga taatttttct
    22981 ttgtcatacc aaataccttg aacccagccg ctaattggtg cagcaatata gctttttcca
    23041 tcttctgaac gaacaaggtt tttggctccg tcataataat tatcttctcc agcttcctcg
    23101 ataacgtttt ggactgccgt ctgatcaatc agttcatctt tatccatcac tttagcaaaa
    23161 tcctgactga cttccattac ttctggtaat ttaccggaac gagctaacgt aacgattttt
    23221 gtattgaacg ctgattcttc taccggcact tgcttgattt tgatgtctgg attttctttt
    23281 tcaaaatcag caaccagttc attgatcaca cttaatcgtt cctgttcgac agaagagtgc
    23341 atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt gactttcacc acaagcagta
    23401 agaagaaatc cagtagctag cagacaggta gtaacaataa tttttttcat ttttttccac
    23461 ctcttatttt attattttca gccacatata ttcatacggc tggaatatca actttcgttc
    23521 ttgactgcaa tggataattt gtccagaaaa caggtctagg taatctcctt ttggtaaaag
    23581 ataacctgct gattgattgg aaaaattatg gataactaag actttctctt ccttcgcttc
    23641 gcgaataaaa ctgataaaag aatctgctga gtccagtaag taaatcaaac tattcggatg
    23701 gaaggctttt tctttttttc gaatagatag catctgggtc aattcaccat atactgctgt
    23761 tcttaatgga tctgtctgca actctttctc aatatctgct aacaaaaatt tttgccgatt
    23821 gatttttcga ttctctcctg ttttttctac gcctttgtaa tcatttcgac ttcccagcac
    23881 actttgaata taaattgccg gcacgcctgg taacgttacc aaaaatgaat gagcagctaa
    23941 aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa cttaacgcat ctagatacgt
    24001 agcgtttatc tcatatggac tggtcgttcc atctgggttt ttcttgtagt ttactcgggc
    24061 accctctttc ttcagctgtt ccactaattc aaggatcgtt ttttccggca gaatacctcg
    24121 taccggattc aatccgatac cgtcatggga ggctaaaaaa ttaaaaaaag ttgttttttt
    24181 cgatgcgaaa tcgatggttt ttaaccattc tttcaactta cttgtattac ccgaacgaat
    24241 cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc atctgtgctt cattctcgcc
    24301 attcccaaaa taactgatat tatctttgtg tggcacattt gtctcagtga tcaataccgt
    24361 tcctggagct gcttcatcag cgattgcacg gaataattgg atgattttat gcgtttctgg
    24421 caggtgtata caagaagttc ctagttcttt ccataaaaat cccacagcgt ccaagcgaat
    24481 aaatgaagcg cctttttcta agtaaaacaa aagaatatcg atcattttca ataaaacttc
    24541 tggattttta aaattcaagt ctacttgatc ttcgctaaaa gtcgtccata cgtgtttttc
    24601 tttcccgttt ttgtcaatga acgttgtaag taacgggctg gtccttggtc tagtcacgct
    24661 gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct ttgtacgtat cattcaagtt
    24721 taaatattcc tgaaaccaag tactttcggc agacatatga ttgcagacaa agtcgaacat
    24781 taaccgtgtt tcgcttctca tttcttctat atcttcccat gtgcccaaac ggtcgttgat
    24841 tgcctgataa tttacaacgg agaaaccatc atcagatgta tatgggaaaa atggcaagaa
    24901 atgtacaact ctaaatgtat ctgccaaata cttttgataa aatttcttaa atgtacgcaa
    24961 agttggttgc tgctcttcaa aaaattgatc cccataagta atcaagaaaa tatcttcttc
    25021 gtcccaatgc agtttgcctg cctggccttt cttctgctga taaacactga ctttttgttc
    25081 aatcatagag gcggttttct cagacaaatc tgagtcgcga taaatcgctt gtaaaagttt
    25141 tgtatacatc ggattcccct ttcttttttg ggagcgttcc caacgatttg atcatattat
    25201 ggaaaacgct tactgtcaat agcatttttt taaataattt tgatatacac aaaaatacaa
    25261 ataagaaagc ttatgtatca ataattaata gcttcttaaa aaaatatggt ctctcatgat
    25321 catgcaagct attcctaaat tttcaaaatc atagacaaaa aagaatgtct attgtactat
    25381 ggaattattc taaaacgctc ccaaaaagga ggtgcatata aaattgaaac ctactattta
    25441 tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc tccagagtat taaataacca
    25501 gccaaatatc tctgataaag caaaagcaag tgtacttcaa gcaatcaaag aattgaatta
    25561 tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt tttgacgcga ttctagcatt
    25621 atctcatcgt acttctcata cgacctatgg caatcctttt ttttcagatg ttattcaatc
    25681 catttctgcc gtttctgaac aaaataactt cgacttgatt ctacaaacgg caaaaacacc
    25741 agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa aaaatgatcc gaggaattat
    25801 tattttaagc tctacgatgg atgaaacact tctttttgaa ttggatcaat acaatattcc
    25861 tattgtggta atcggaaaag tcggaaacga ctacacgaac atctactcag tggacacgga
    25921 taatttccag gatagttacg atttggtgga aacactgatc aagaatggac accgtcatat
    25981 cggctgtctc catgctccga cagaagttta tgtatccaat gatcgtctca atggttaccg
    26041 tcaatgctta tttgatcacc acctagatat ccgtaatgaa tggattatag acggcggctt
    26101 tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg aagctacgag aaagaccaac
    26161 tgcgattttt tctaccgatg cattaaaatc attgagtatt tacaaagtgg cagaacaatc
    26221 tggaaagcag atacctgatg atctttcgat cgcaggattc agtgatccct tattgtcctc
    26281 cctattttcg ccacagttga ctcggataga cattccgaca aatcagttag gaatcaaagc
    26341 cacagaactt ttattccaac taatccacca ccaataccct gaagaaaaaa gaatcattct
    26401 ttcaaccgag attactctca cagattcgat ccaacaacaa gaaatgtaga acagaattat
    26461 aaaagaacag aaacactgag gataagtcag ttactctgtt cttttttcaa tcactcatcc
    26521 ctcgttaccc cctcccggta ttttagcgaa tttttcttat tttccatctg ttttagatag
    26581 ctgtggataa ctataatgtt tgtgttaaac tattttttcc acagggaaga aaaaactatt
    26641 tttaaccacg ttgtgtataa gtttttatag ttcgtttttt taaacacatt tacccttatt
    26701 tcaatccatg ttatttgggg atacttatct ctaaaacata aatagaataa cagacatgtc
    26761 tactggtttt gttttattcg aggaattatt caataatagc tagcaaaata ttaaaaataa
    26821 ccgctctatc ttcccatcaa tttattttgc attcaaaaaa tgatctatct ttacttttgt
    26881 tgattgactt ctcttggaag atgatcgttc cgatacctta attggatacg aaccgatgtt
    26941 gttaagatcc tttcccactt ttcttctagt tagacaaatt attttagaca aaccaaaaaa
    27001 gaggttgtga caataatttg tcacaacctc ttttttaaaa tatctatttt gtttgattgt
    27061 tcattgcgtc ttctgcagcc atttgcgctt caatatctga aatactgtag acattttcgc
    27121 ttgctacacc gacttctttt ggttccatgt cacgataacg ttgcatacca gtaccagctg
    27181 ggatgatctt acctataata acattttctt tcaaaccaag aagaggatct ttcttaccgc
    27241 ggatcgcagc atccgtcaag acacgagttg tttcttggaa tgatgcagca gataagaaac
    27301 tatttgtttc taatgatgct tttgtgatac ctagtaagac aggtcgagaa gttgcaggaa
    27361 cgcctcctga gactagcgta ttgtagtttt gatctttaaa gtctgcgatg tctaataatg
    27421 tacctggtaa gatatctgta tcacctggat ccattacacg aactttacgc agcatttgac
    27481 gaaccattac ttcgatatgc ttgtcgccga tttctacccc ttgcatacgg taaacacgtt
    27541 gtacttcacg aagcaagtag ttttcaacag ataatacatc gcgaacttgc aacaattgtt
    27601 ttggatcgat tgatccttct gtcaatggtg caccacggtg gatcatatcg ccttctgcta
    27661 ctttcatacg tgctgtataa ggaacagtat acgtacgtgt atctgttttt cctttgattg
    27721 ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc gatgacatcc cctgttactt
    27781 ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa gatttcttgg acacgaggaa
    27841 gaccttgagt gatatcgtct ccggcaaccc cacctgtatg gaatgtacgc atcgtcaact
    27901 gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc aactgcttct ccgacttcaa
    27961 cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt gcagacacca tgttttgtat
    28021 tacatgtgaa gacagaacgg attgtcactg tttcgacacc tgcatcaacg atttgttttg
    28081 cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa ttcatttgtt tcaggatgac
    28141 gtacttcttt acgggtgtaa cgtcccacta aacgatcttc taatgattcg atgatttcat
    28201 ttccctcgcg gatggcttgg atttcaagac ctcggtcagt accgcaatct tcttcacgga
    28261 tgattacatc ttgggccacg tcaaccagac gacgagtcaa gtaacctgaa tcggcagtct
    28321 tcaaggctgt atcggtcatc cctttacgag ccccgtgagt tgagataaac atttccaaga
    28381 cggatagtcc ttcacggaag ttcgaaatga ttggcaattc catgattcga ccattaggag
    28441 cggccatcaa accacgcatt ccggcaagct gtgtaaagtt ggaaatgtta ccacgagctc
    28501 cagaatcaga catcatgaag attggatttt tcgcttccaa accttccatc agtttttgtt
    28561 ggatgctgtc ttttgcagca ttccaaacag caatgacacg ttcataacgt tcgtcatctg
    28621 tgatcagacc acgacggaat tgttttgtaa tagtttcaac ttgtttatgg gcattttcaa
    28681 tgatttcttg tttttcattc agtacactga tgtcagcgat ccctactgtg ataccggcat
    28741 gtgtcgaatg tttgtaccct aagtctttca tacggtcaag catcttagaa gtttctttta
    28801 ctttgaatcg tttgaagact tctgcgatga tgtttcctaa gtttttcttc ttgaacggta
    28861 atactaattc ttgctctttg atatgtgcag gaatatcagt accagcttca acaaagtatt
    28921 tgtccggtgt ttggacggtc aagttgaagt ctgttggttc attcagataa gggaattcag
    28981 gaggcatgat ctcgttgaag atgatcttac caactgttgt gatcatcgta cgttcttttt
    29041 gccattcagt aaatggtttt tcacctaatg agttagggtt cacaccgata cgtgaatgta
    29101 aatgaacata tccgttgcgc catgcgataa cggcttcgtt ttgatctctg aagaccattc
    29161 cttcgccttc gcgtccttct tcttccattg tcaagtagta gttacctaga accatatctt
    29221 gagaaggagt aacaactggt ttaccatctt tagggttcaa gatgttttga gcagctaaca
    29281 tcaacatacg tgcttctgct tgtgcttctt cattcaatgg aacgtggaca gccatttggt
    29341 ctccatcgaa atcggcatta taagcttcac atacaagtgg atgcaaacgg attgcgcgtc
    29401 cttgtactaa aactggttcg aatgcttgga taccaagtcg gtgaagcgta ggtgcccggt
    29461 tcagcaatac tgggtgttcc ttgattacgt cttctaaaac atcccatact tcgtcttctt
    29521 gacgttcgat tttacgtttc gcatttttga tgtttgaagc tagttcgcgt tgaacaagct
    29581 cacgcatcac gaatggtttg aataattcaa tggccatttc ttttggaagt ccacattggt
    29641 acatcttcaa gaaaggtcca acaacgataa cggaacgacc tgagtagtcg acacgtttac
    29701 ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa catatgagaa agagatttca
    29761 atgggcggtt acctggtcca gtaactggac gtccgcgacg accgttatcg atcaatgcat
    29821 ctactgcttc ttgcagcata cgtttttcat tttgaacgat gattccaggt gcattcaagt
    29881 ctaacaaacg tttcaaacgg ttattacggt tgattacacg acggtaaaga tcattcaagt
    29941 cacttgttgc aaaacgtcca ccttctaatt gaaccattgg gcgtaaatct ggtggaataa
    30001 cagggataac gtccataacc atccagcttg gttgatttcc tgaagcgcgg aatgcttcta
    30061 aaatatccaa acggcggatt gcacgtgtac gtttttgtcc ttgtgcagat ttcaattctt
    30121 ctttcaattc ggctgcttct gcatctagat caacattatc aagtaattgt ttaatcgctt
    30181 ctgcacccat agcagcttgg aattcttgtc catattgttc acgtttttcg cgatattcgc
    30241 gttctgtcaa caattgtttt ttctctaaat tcgtatttcc tggttcgatg actacatatg
    30301 aagcaaaata aatgatttct tccaaagcgc gtgggctcat gtctaataca agacccatac
    30361 gtgaaggaat ccctttaaag taccagatat gtgaaacagg agctgctaat tcaatatgtc
    30421 ccatgcgttc acggcgaact ttcgaacgag taacttccac tccgcatcga tcacaaacga
    30481 ttcctttata acggatacgt ttgtattttc cgcaggcaca ttcccagtct tttgtaggac
    30541 caaaaatccg ctcacagaac aacccttcgc gttcaggttt taaggtacga tagttaatcg
    30601 tctcaggttt ttttacttca ccgtaagacc agcttctgat tttttcggga gaagccaaac
    30661 ctatttgcat actttcgaat ttatttacat cgatcaaaag gggttccctc catttcattt
    30721 aatggcaaac cgcaagacgg ctgatcttcg accgccttgc agctcataga ccggattagt
    30781 ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc gtctttttca tcttccactt
    30841 gttcagcagc tttcttttct aattctttgg cagtttgctg ttcggcaaat tttgtcaaag
    30901 catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc gcgaagttcg atctctgttt
    30961 cttcgatatc caagacacgc atgtccagac ctagtgattg caattctttc accaatacgc
    31021 ggaatgattc cggtacacct ggttttggaa ttggttcacc cttgacgatt gcttcgtatg
    31081 ttttcacacg tccgactacg tcatctgatt tgtatgtcaa gatttcttgt aaagtataag
    31141 ccgcaccgta agcttccagt gcccatactt ccatttcacc aaaacgttgt ccaccaaatt
    31201 gagctttacc tcccaatggt tgttgtgtaa ccaatgagta aggtccgatt gaacgtgcat
    31261 gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta catcacaccg acagacacac
    31321 gtccatcaaa tggttcacct gtacgtccgt cgtacagaac agttttcgca tcgctggcca
    31381 taccggcttc acgaactgtt tcccatacgt cttcatcact tgcaccatca aatactggcg
    31441 ttgctacgtg gatacctaac tgacgtgcag ccatacccaa gtgtaattcc aatacttgcc
    31501 cgatgttcat acgtgaaggc acccctaaag gattcagcat gatatcgatt ggtgttccat
    31561 caggtaggaa tggcatatct tcttccggca taatacggga tacaacccct ttatttccgt
    31621 gacgaccggc cattttatcc ccttcatgga ttttacgttt ttgaacgata taaacacgaa
    31681 ctaacatgtt tacgcctggt gacaattcat ctccagcttc acgagtaaag attttcacat
    31741 cgtgaacgat accgccgccg ccatgtggta cacgtaatga tgtatcgcgg acttcacgag
    31801 ctttttcgcc aaagatcgca tgcaatagac gttcttctgc agataattct gttacccctt
    31861 ttggcgtgac tttccctaca agcaaatcgc catcttggac ttctgcacca atacggatga
    31921 tacccatttc gtctaaatct tttaatgcgt cttccccgac attagggatt tcgcgtgtga
    31981 tttcttcagg tcctaatttt gtatcgcggg cttctgattc atattcttca atatggatag
    32041 aagtgtatac gtcatctttt accaaacgac ggctcatgat gatcgcatct tcatagttgt
    32101 acccttccca agtcatgaaa ccaactaaaa cgttttggcc tagagccatt tcgccttgtt
    32161 ccatagatgg tccgtctgcc agagtatccc ctttttcgac tttctcaccc aaatgaacga
    32221 ttggacgttg gttgtagctt gttccggagt ttgaacgacg gaattttgtt acatcgtatt
    32281 tatctaatgc accgttgtcg cggcgtacgc ggatttcaga agcatcaacg tattcaacga
    32341 caccatcatg tttacataac aaagcagctc ctgagtcatg cgctgatttg tattccatac
    32401 cagtaccaac ccatggtgct tgaggattga tcaacggcac tgcttgacgc tgcatgttgg
    32461 cacccatcaa ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa
    32521 cggcaactac ttgtttaggt gatacgtcca tgtagtctac tttatcaata gatacttcta
    32581 agttttcact ttgagcacga gccataacaa cgtcttgagc aaatgttcca tcttcattca
    32641 atgggctgtt tgcttgggct acgatgtaat ggtcttcgat atcagctgtc aagtagtcga
    32701 tttggtcagt aacacgacct gtttcacggt ctacacgacg ataaggtgtt tcgataaatc
    32761 cgaatttatt tactttcgca taactggaca aactattgat caacccgatg tttgggcctt
    32821 caggagtttc gatcggacac atacggccat agtgggagta gtgaacgtca cgaacttcat
    32881 aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc gaaaagacga cgtttgtgtg
    32941 tcaattcgcc aagagggttt gtttgatcca tgaactgtga caactgagaa gaaccaaaga
    33001 attctttgat acttgccaca actggacgga tattgatcaa ttgttgaggt gtcaatgttt
    33061 cagtatcttg gatcgacatt ctttcacgaa ccacacgttc catacgtgct agcccgatac
    33121 ggaattggtt ttgtaaaagt tcaccgactg aacggatacg acgatttcct aagtggtcga
    33181 tatcatcgac attgccgata ccttccatca agttcaagaa atagctcatt gaagcaatga
    33241 tgtcggctgg acgaaccgtt tttacagctg cttctggata gccgtttccg atcacgttta
    33301 cttcacgttc agggtctctt ggtgaaaata ctttgatgac ttggacagtc attggatctg
    33361 tgaccacacc gtcttctgat ggatagtaag tcactgaatt taggccatta tcaatgaaag
    33421 gagctagtgt ttccatgact tggtgagtca gaactgttcc tttttcaacg atgatctcgc
    33481 ctgtttctgg atcaactaat gtttcagcta atgtcagatt caatagacgt gttttcaaat
    33541 ctaatttttt gtttactttg taacgaccaa cgtttgctaa gtcgtaacgt tttggatcga
    33601 agaaacgagc attcagcaag ttacgtgagc tgtctgctgt ttttggttcg cctggacgca
    33661 aacgttcgta aacgtctttc aaaccttctt ctgttcttga gtcgcttgcg tttttatgca
    33721 agtctttttc gactgtattg cgtaatgttt cgctgtcgcc gaagatttca aagatcgtat
    33781 catcggaacc aaatcctaac gcacgaacta atacggttaa aggaatctta cgtgtacggt
    33841 cgatacgtac ataagagatg tcttttgcat cagtttccat ttctagccaa gctccacggt
    33901 tagggataac agtagaaccg aatccttctt ttccgttttt gtcgactttt ccatggaaat
    33961 agacacctgg tgaacggact aactgagaaa cgattactcg ctctgccccg ttgatgatga
    34021 atgttccttg ttctgtcatt aatgggaaat caccgaagaa tacttcttgt gctttgattt
    34081 cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa tggtgcagag tagtttgcat
    34141 catgcgcacg agcttctgct actgtatact ttggttcttt caattcataa tctacaaatt
    34201 ccaaagatag attgccattg aagtcatcga ttggcaaaat gtcttcaaac atttctctaa
    34261 gtccctcatc taagaaccat tggtaagagt ctgtttgaat ttcgatcaaa tttggtaatt
    34321 ccaacacttc actgattctt gcgaaacttc tacgttcgcg atgttttccg tattttacta
    34381 cgtgtccagc caagctcttc acccctctaa aatgtcattc aaaaagacta cccgatcaat
    34441 tgttacaata atatttcaaa tattattttt aggtaacaac agacattttt tgcggctttt
    34501 aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc ttacttcgtg agcttctact
    34561 tttgtttcct tatattaaag ccgaaacgga caatatttcg tttctgcctt ttcttcatca
    34621 gatagttttt gcaacttcat atactacact tattttctat aaaagtcaac acttcctggc
    34681 gtttcttctg ctttttcgca acattttctt aatgttttca attttatgtt ttcaattatc
    34741 tgacaattct taattgacag acctttttat ctcggttata ctttagaaaa ttcattttgt
    34801 ggaggaactt ctatgcgaac caacatcaac caaatcgcac caacacaact gaataacttt
    34861 tactttagct actttagata ggtttgtatt catcatgatg gatacaaacg ttaaacgaca
    34921 gtttgtatct atgatggcta aagaactttg tattgcagca aaaagccacg tagacaaaat
    34981 gctaagtggc tgagcagtga aaatacaaag taaaagtatc aagcattcta ctttttcaat
    35041 ttattttccg acttttcaat ccatttggac attttgtctg aatggatttt ttgtttccca
    35101 aaattaagga ggaaaaagaa atgaaaaaaa atataatcat cggattaggc gctgccctgc
    35161 tactagcttt aggaggttgc gcacaaaaag acaattccgc agaggcatca gatggacaaa
    35221 aaacaattgg cgtattgcaa cttgtacaac ataactcgtt agatgctgcc tataaaggat
    35281 tcaaagaagg attagcagaa gaagggtata aagaaggcga taatctaaca atcgattatc
    35341 aaaatgccca aaacaatcaa gacaacctga aaagtatgag tgagaaactc gtcaaagaag
    35401 atcctgattt gctacttgga atcgccactc cagcagcaca atctttatta aatgaaacaa
    35461 ccactatccc gatgactgtc acagcggtca cagacttgga acaagcccat ttagtcacat
    35521 ccaatgaaaa acctggcggc aatgtgactg gcacgacaga tatggtgcca atcgaaaaac
    35581 aaatcggctt actgttaaat atcgtgccag acgccaaaac aatcggcatc atgtataaca
    35641 acggtgaagc aaactctaag atccaagctg atttggcaga aaaagaattg aaaaaagcgg
    35701 gcgtcaaagt caaagtactg acagcaaatt ccacaaatga tgtccaacaa gtgacgaaaa
    35761 gtctagctaa agatgtagat ggaatctata ttcctactga taacacgttc gcaagtgcgg
    35821 caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc tgtcgttgct ggcgcagttg
    35881 atcaagtcaa agacggcggt ctagcaacag taggaatcga ctacgaaagc ttgggcaaac
    35941 aaacagggat catggcagca aaaatcttag acggcaagaa tccaagtgaa cttcctgtcg
    36001 aaacagcgaa tgatttggaa ctgtatgtca ataaagacat ggccaaagca ttaggcatca
    36061 atccagacag catcaaagaa gctgaataaa acacaggagg attctcatga caaatttgct
    36121 ctctcttttc ttatcatctg catcgcaagg ggtcttatgg gccctacttg cgattggtgt
    36181 atacgtcact ttccgtatct tggacattgc cgacttgact gctgaaggaa gctttccttt
    36241 aggtgcaggg atcgcggcag taagtattac aaatggctat catccattga cagcttgtct
    36301 tctaggcttt ttagggagtg cggcagcagg gcttgtttcc ggactgcttc acacaaaatt
    36361 gaaaattccc gcccttcttg cagggatcat caccatgact ggtttatatt ctgtcacatc
    36421 ccgtgtgatg ggtgcgccga acatctcttt attgggtcag ccaacgatct ttcattgggt
    36481 cgagtcattc ggcataacaa aagacaacgc tgtactgatc gtcggtctgc tttttgcttt
    36541 gatcgttgta tgtttacttg ttttgttctt acgtacagaa acaggacttg ctattcgtgc
    36601 aactggcgat aatctggcaa tgagcgaagc aaacgggatc aacacagata atatgaagat
    36661 catcggttat atgatttcta atggatgcat tgctctttct ggctccttgc ttgcgcaaaa
    36721 caacggcttt gctgatttaa attctggtat tggaacgatc gtaattggac ttgcttctat
    36781 tatcatcgca gaagttttgt tacgtaacca gccactattt ctccgtctct tgactgtcgt
    36841 tttaggtgcg gtcatttatc gttttatttt agcgttagtc ttcgaactga atgttgaacc
    36901 aagtgattcg aaacttgctt ctgctttcgt gctagtgatc tgtctttctt tcccgcagat
    36961 ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt agtgtcaaat gaatgacgtc
    37021 ttaacattgg aaaatctcca tcataccttt gaaaaaggaa cgatcaacga aaatcatgtc
    37081 ctgaaaggca ttaatctttc cttagatccc ggcgacttcg tgacgatcat cggtggaaat
    37141 ggggctggta aatctacttt gctgaacagc atcgccggta cattttctgt cgatcagggt
    37201 caaattttat tgaatggaaa aaatatcacg aaaaaatctg ttgtcgaacg ttcgaaatcc
    37261 atcagtcgcg tattccaaga tcctaaacta gggacagccg ttcgtttaac ggtagaagaa
    37321 aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct tcttccgtgg tgtcaaacca
    37381 caagaccgaa catttttcaa agaccagctg gttcggctaa atttggggtt ggaaaatcgt
    37441 ttatcctcag aaatcggttt gctgtctgga ggacaacgtc aagctattac gttattgatg
    37501 gccacattac agcgtccaga actgatctta ttagatgaac acactgcagc gcttgaccct
    37561 gctacttcca aaacggtgat ggaattgaca gaaaagctga tcactgagca gaatctcact
    37621 gcttttatgg tcactcatga catggaagac gcgatccgct atgggagtcg actgatcatg
    37681 ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg aaaaacagca gctcacagtg
    37741 ccccaattat tagcgttgtt caaacaaaac agcggcacgg cattaaaaga cgatcaagta
    37801 cttttatctt cataatcaga aagaggatgg gacaaaaaat gaacgcccac cctcttcttt
    37861 tttctctttc cttctttcgt ttttgcttta tgtatcaacc gttgtttctt cgaaaactat
    37921 tttgttctct ccttcttcct gatagaatgg atgggaaagg agcttgatgc catgacaacg
    37981 aaagacaagg tacttcaatt attgaaaaag acaccagatt ttctttccgg agaaaagttg
    38041 gcacagcaac tggaagtttc ccggacaagt atttggaaag ccatcaaaga attggaaaag
    38101 gcaggctatc gttttgagca cgggcctaca gggtatcgtt acctgccctc tgacgtacta
    38161 gatgcaaaag aaatccagca agagctacgg tcgtttattc ctgatttgaa agtacaaacg
    38221 atgatgactt ctgaatctac gatgaaagat gccaaactgg cagcagctca aggagattct
    38281 gtttcgaagt tatttgttgc tgatacacaa gaacaagctc gtggacgttt tggacggcct
    38341 tttttcgctg aacctggacg aggaatctac atgagtctct tgctccatcc agacaaacgg
    38401 tttgatgaat tgcctcaata tacgatcatc gcagccagtg cctgtgtgaa agccattgaa
    38461 tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg atatctacat ggatggaaaa
    38521 aaagtctgtg ggattttatc tgaagcagtc agtgatatgg aaagcggcag gatcagtcat
    38581 atcatcattg ggatcgggct taatttttct gttcctcaaa atcattttcc agctgatctc
    38641 caagaaaaag ccatctctgt ctttcccgac ggacaagcta gtgtcacaag gaatcagcta
    38701 atcagcaaaa tctggcaaga attgttcact tatctttctc acccagaaaa agcgttggac
    38761 ttctataaag aaaaatcatt cgtattggga aaaaccgtgt cgttcaatca aaaaggaacg
    38821 acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg aactgatcgt ccaaacaaat
    38881 gaacagaaga tgagtttgtc ttctggcgaa atcagtctca catctatccg tacgcaataa
    38941 ttatcagatt tcacaaaagg acacctgatc tcatacagtg tccttttttc tgaattctct
    39001 tcaaaagact ctccttattc tatcagctga cagtttctct atcctctaga aaaaggtatt
    39061 ctaaaaatat ctataaagca tggtacactt tctttaaatg aatcaatgca aaaaacaaga
    39121 gagtcatcag tttacacgat caaaagagat agcaggccgc ttcctttttc aatcttatag
    39181 atagaccatg ggtaacgata aaaacaaaaa agaacttgcg acttattttg ccgtaagttc
    39241 ttttttagct agtgccttaa tctcttaatc ccaacaaacg tctcgcatag atcacctgac
    39301 cagagtgcat agcagcatca tcaataatag aaacgagacg cattcctcgt gtgactggtg
    39361 gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg ttccagcctt cttaagtaag
    39421 aaatagcaaa atcagcagct cctcgcaagt atcctagcag atcttctatt tgattagctt
    39481 ggatctgctt tgcttgttcc agactatgct tccaatcttg ttcgttatct gctatcgtca
    39541 tcggaaaccg gctttcccag tcttgactat gccaaattgg agaagttcct gctaaatcag
    39601 caatctggaa atctaattct cgagcggtat gccaagccag ccaagtcatt gatttgatct
    39661 gcggggctct atcttccact ggaaaacgat tggcctcctc aatagagacg ccttcaaaca
    39721 aacgttcaaa ccgatcgtat gcccgatcca cactctcgca tagcatatct aaataatcca
    39781 tttattttcc tcctttttat ttatcctact tactttttcc taattctagg atacctactt
    39841 atatgttact acacaccctt attttttaca ctttaacagt caccatcatc aatagtttgg
    39901 agattgaaag aaaaagatag tgtcgaacta tcaattgaac aagaagcaac aatatgaacg
    39961 tttcattaat attctttaaa taaacaatga tttttcaaac gaacagttta agactaggag
    40021 aaaaagagta taataggaaa atgtttgaaa ggaaaggaag gggaaaaatg aaaaaaaggg
    40081 caagtttact agtcctagtt gctttgttgt tcaatatctt ttcgtatggt gtggccgttt
    40141 cagcggaaac atacattcct aaagaccctg ggaatattta taatgatcta actggtaact
    40201 atggcttgtt aggaattgct agtcaattcc acgtatttgc taaaaataag acaactgtga
    40261 atgcacatac agacggaaat atagcaacac atgaacttga tgcaaaaaat aattttggta
    40321 cagagcttca tgaaggttta ttgaaccaag aaataaatta tatacaaaaa actgattctt
    40381 tcgctaattc ttcaggcata cctacaggta gcacaagaat gaataaattt gtcgttggca
    40441 gtaacgtgga tgtagcactt caagatggtc atcctacagt caatggatcc aggcttgatc
    40501 acttaactcc agatgatttt tataaagatc gtgctgggca tgaatatatt gatttcgagc
    40561 aggaatttgc taaattgaat gtagcagcaa atgatcttgc aacgatcact ccagctaaaa
    40621 catatactgc tgcagatttt ccagacatga acaatcggac aatcgattta actgatttat
    40681 cagataccgg gtttttattg gtaaatatcg atgcagaagt gttaacgatg aatacgccac
    40741 ttcaaatcat taatccaaat gatcaagtag ttgtatttaa tgtcatcaac agttcttctg
    40801 cattgaacgt acagtcacca atcaaataca atgatcgttc aaatcatgaa acagaagatt
    40861 tttcggatgc gaatatctct tggaattttg gaaatgagat gactgatttg acaatcagtg
    40921 ctccttttca aggaacaatc cttgcgccta atgctactat ccgcattact caaaaccaag
    40981 acggaacgat tataggaaca aatgtgattt tagatgcagc taccaatcgt tgggatccta
    41041 acgaaatatt tatcagcgac aatggtacag atacgacaga cacaagcgac agtacagata
    41101 catctaccac aagtgatagc tcagattcat caacttcaag cgacagtaca gatactacct
    41161 ctaccacaag tgatagcacg gatacatca
//
LOCUS       DAZKAY010000012        40825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000012 DAZKAY010000000
VERSION     DAZKAY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.012"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(326..1603)
                     /locus_tag="ACG8WP_000602"
     CDS             complement(326..1603)
                     /locus_tag="ACG8WP_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026937.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1682..2326)
                     /gene="pcp"
                     /locus_tag="ACG8WP_000603"
     CDS             complement(1682..2326)
                     /gene="pcp"
                     /locus_tag="ACG8WP_000603"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9026938.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2525..3469)
                     /locus_tag="ACG8WP_000604"
     CDS             complement(2525..3469)
                     /locus_tag="ACG8WP_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9026939.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3471..4157)
                     /locus_tag="ACG8WP_000605"
     CDS             complement(3471..4157)
                     /locus_tag="ACG8WP_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9026940.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4631..4951
                     /locus_tag="ACG8WP_000606"
     CDS             4631..4951
                     /locus_tag="ACG8WP_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9026941.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5105..6010
                     /locus_tag="ACG8WP_000607"
     CDS             5105..6010
                     /locus_tag="ACG8WP_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9026942.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6007..6780
                     /locus_tag="ACG8WP_000608"
     CDS             6007..6780
                     /locus_tag="ACG8WP_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9026943.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6961..8385)
                     /locus_tag="ACG8WP_000609"
     CDS             complement(6961..8385)
                     /locus_tag="ACG8WP_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9026944.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8596..9042
                     /gene="nrdI"
                     /locus_tag="ACG8WP_000610"
     CDS             8596..9042
                     /gene="nrdI"
                     /locus_tag="ACG8WP_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9026945.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9107..11089)
                     /locus_tag="ACG8WP_000611"
     CDS             complement(9107..11089)
                     /locus_tag="ACG8WP_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9026946.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11103..11999)
                     /gene="murQ"
                     /locus_tag="ACG8WP_000612"
     CDS             complement(11103..11999)
                     /gene="murQ"
                     /locus_tag="ACG8WP_000612"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9026947.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12002..13063)
                     /locus_tag="ACG8WP_000613"
     CDS             complement(12002..13063)
                     /locus_tag="ACG8WP_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9026948.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13274..13801)
                     /locus_tag="ACG8WP_000614"
     CDS             complement(13274..13801)
                     /locus_tag="ACG8WP_000614"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9026949.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(13868..15370)
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_000615"
     CDS             complement(13868..15370)
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9026950.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15628..16749)
                     /locus_tag="ACG8WP_000616"
     CDS             complement(15628..16749)
                     /locus_tag="ACG8WP_000616"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9026951.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16875..17816
                     /locus_tag="ACG8WP_000617"
     CDS             16875..17816
                     /locus_tag="ACG8WP_000617"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9026952.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17966..18862)
                     /locus_tag="ACG8WP_000618"
     CDS             complement(17966..18862)
                     /locus_tag="ACG8WP_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9026953.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19131..20471
                     /locus_tag="ACG8WP_000619"
     CDS             19131..20471
                     /locus_tag="ACG8WP_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9026954.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20689..21132
                     /locus_tag="ACG8WP_000620"
     CDS             20689..21132
                     /locus_tag="ACG8WP_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9026955.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21636..23054)
                     /gene="nhaC"
                     /locus_tag="ACG8WP_000621"
     CDS             complement(21636..23054)
                     /gene="nhaC"
                     /locus_tag="ACG8WP_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9026956.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23457..24680)
                     /locus_tag="ACG8WP_000622"
     CDS             complement(23457..24680)
                     /locus_tag="ACG8WP_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9026957.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24711..25550)
                     /locus_tag="ACG8WP_000623"
     CDS             complement(24711..25550)
                     /locus_tag="ACG8WP_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9026958.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25567..26247)
                     /locus_tag="ACG8WP_000624"
     CDS             complement(25567..26247)
                     /locus_tag="ACG8WP_000624"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9026959.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26249..27322)
                     /locus_tag="ACG8WP_000625"
     CDS             complement(26249..27322)
                     /locus_tag="ACG8WP_000625"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9026960.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(27378..27616)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(27659..27892)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27969..28640)
                     /locus_tag="ACG8WP_000626"
     CDS             complement(27969..28640)
                     /locus_tag="ACG8WP_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9026961.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28727..31375)
                     /gene="mgtA"
                     /locus_tag="ACG8WP_000627"
     CDS             complement(28727..31375)
                     /gene="mgtA"
                     /locus_tag="ACG8WP_000627"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9026962.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(31717..31891)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            32064..32501
                     /gene="ndk"
                     /locus_tag="ACG8WP_000628"
     CDS             32064..32501
                     /gene="ndk"
                     /locus_tag="ACG8WP_000628"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9026963.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32523..33389)
                     /locus_tag="ACG8WP_000629"
     CDS             complement(32523..33389)
                     /locus_tag="ACG8WP_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9026964.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33549..35642)
                     /locus_tag="ACG8WP_000630"
     CDS             complement(33549..35642)
                     /locus_tag="ACG8WP_000630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9026965.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(36391..37014)
                     /locus_tag="ACG8WP_000631"
     CDS             complement(36391..37014)
                     /locus_tag="ACG8WP_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026966.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37173..38570)
                     /locus_tag="ACG8WP_000632"
     CDS             complement(37173..38570)
                     /locus_tag="ACG8WP_000632"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9026967.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(38602..39573)
                     /locus_tag="ACG8WP_000633"
     CDS             complement(38602..39573)
                     /locus_tag="ACG8WP_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9026968.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            39947..40372
                     /locus_tag="ACG8WP_000634"
     CDS             39947..40372
                     /locus_tag="ACG8WP_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026969.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        11805 a         8544 c         7136 g        13340 t
ORIGIN      
        1 gtgtcgtctt tgtataggac actgtagctg tgtcccattg aaacatctaa tgcaagaatg
       61 taaggcattt gttttcctcc tttttaagtt ttgaaagaac tttactgctt catttcgatt
      121 ccaatttcct atacacgggc tcatggcccc aacatacgta aacctgattc taaaatgaca
      181 gtaaagaagt tcggtttttg atagggactc gtagtcctca aggctttgtt cgaactttct
      241 ttcactcttc actataatct aaaaatagaa aatagaggag aacatcattc gtcaatgatg
      301 ttcccctcta atcttagtat gttttttata gtaattcctt cagatacttt cttgcctcat
      361 cattgaaacg cagtgattct tctaacttct gataatattc caacgcttgt ttctgagctt
      421 cagtaaaacg ataatgtttt cctttccatt tgtcgattgt ctcttgcaca tttccagatt
      481 ggatatcttt cactactagt tgccattttt cttttgagac tgtacggtct ttcttatcag
      541 cagattgtat agcttctcgt tctttacgca atacttttaa aaaaccattt ctgactatcc
      601 gaggaatgct gtcgaagaac ccttctgtaa aaccatagcg acaatattgc ttccaaataa
      661 atagatagat ttttggttgg atgaatcgtt ggtttttcgc aaaatattcc atctgagcag
      721 tgacttctgg tgttcgttga cgttttgtct ttctcaacac ggaaataaat gtttcaaaat
      781 aagcattagg atgtactggc acttcttcat atacgatgga ttgccaatct ttacgtaaaa
      841 atgcgcgata gttgatcaag aattcttcta catcctttac taattcttct gcaaagtcgt
      901 caaatagttc tttttttatc tgtcgctttt ttgactgccc attgatgtgt tcacttattt
      961 tcttgatttc attttccaat gcttgataat ccgctatgta aatcggcatt tctacttggc
     1021 gaaattctgc gagccgctga gctaatttta tatccggtaa atatggctgt ttcatccgac
     1081 gattttggta caagacaaaa cagtattttt gccatagtcc ttcaggaaaa tcgatattct
     1141 tctgcttaag ccataataat tcgtctaagg ccagatcaac ttttgtcatt ccctgatgga
     1201 tcgaagctgc cactgtcgga gagacatgca ggtgatcggc aaagtggttg atgcctagtt
     1261 ttaggatctt tccggttttt ggcgatttaa cgatgtattg cacttttaat tctctaccac
     1321 actcacaata caatttttgc tggcttcgtt ttgttcgaaa aaacggattg atcttgaagt
     1381 cagaaaacac ccaatctgtt gcagctaaat aatgttcttc cataaaacga gagctgatta
     1441 gatatcggcg gtgcttatcg actagttccc gctgtttttt cgttaatttt gcatagatct
     1501 cccaacgttg actgcttgtg tattgtttag aaattttgat gggatagctt ttgctgattt
     1561 cttttggaaa ccattcttca aagacggatt gccattcttc cacggctgtt tctttccttt
     1621 ctaattacaa aaagctggct actaacataa cgttagcaac cagccagtgt ttcttgctaa
     1681 attagtgagt tgttccggca tttccggaaa tatcttcttt attttctact gctgcttcaa
     1741 ctgcataagt caaagaagaa gcaatgtctt ctaaagacat agaaggcgta cccactggac
     1801 gatcgatcac ttgatcgggt gagaatggca cgtggatgaa cccacctcga attgccggga
     1861 attgtgtttc gatcaaatga agcaaacgat acatgatatc attgcagaca aatgttcctg
     1921 ctgtataaga gatgtacgct ggtagaccat gatcttgtac attttttgtc attgctttga
     1981 ttggcaatga ggtaaagtaa gcggctggac cgttttcttc taaagccgta tcgaatggct
     2041 gatttccttc attatcaggg attcttgctt ctgctagatt gattgcgaca cgttcgaacg
     2101 agactgctga ccgcccacct gcttgtccga cacagatcac gacatctggt tggtgctgtt
     2161 ggatggcttc tttgactact tgactgcttt tttcgaatac ggtcggtact tccactttga
     2221 tgatttcagc accgctgatt tcatctggca ttttttttac tgcttcatag gctggattca
     2281 ctttgtcccc accaaatgga tcaaaacctg ttactagtac tttcatctgt tgttcctccg
     2341 tccgattgat ttaacatatg gttatctttt atcataccat tttccttgaa cagcgtaaat
     2401 aaaggaaaca atagtttaaa taacataaca gatcaaactt aatatattct tataaaaaaa
     2461 tagtgttcca ctacaatgtt agaagaggtt gcgacaatta ttctgtcaca acccctctct
     2521 tctattattt aaatagaatc atataagcaa tttggaaaac tagcataaat aacgcaataa
     2581 acaactggtt tttgatcact ccgtatttgt ctttcatctc cagcatcgct acaggcacga
     2641 tattgaagtt agcagccatc ggtgtcagca gggtcccaca aaaaccgcaa gtcaatgcaa
     2701 ccattcctat taacgctgga ttcgctccat gactaaatac gaatggtgca ccgatcccaa
     2761 ccgtcatcac ggtgatggcc gcaaatgcat tccccatgat taccgtaaat atcaccatgc
     2821 caagagcgta aatgatgatg ccgatgttca catttccttc tggaacgatc gtacctactg
     2881 cattggaaat cactgtccca actccagcgc ttgtaaatac cgcacctaga gaagctaaaa
     2941 gcattggcaa catgcttaac ggccctactg tgcccagcat atctgctgca tcatctaaaa
     3001 atgtacttgg tcgattatct cgagaaaaga acatcaagat tagcatggct gcaaaaacac
     3061 cgacgcccac gccgactaaa gcacctaaat tggtaaatag agcaaacaag atcgcaaaga
     3121 caccgatact caaagctgga ataaaaattt tcattcctag tttgtctgac attttttcca
     3181 tatattcttt tgatggaagc cggctttctc ctttgctgac tttcttagca attgctggta
     3241 tggtcatcgc aaagatcaag ccaccgttca cgattgccgg aagccatctt cctcctgcaa
     3301 taactacccc taagaccgtc cagaaaaagg ctgacccata acgatgagga ttggttttat
     3361 ctgttaaatt tcttactcca gtgtaaatca atacgagtcc catcacgata taaaggatct
     3421 ccagtaattt atcgcctaat aaaatatcag gatttgtaaa aaagtccatc ttattgcccc
     3481 tttcctggat aatatttctt cgctaatttc ttttctttaa gatagaagta gacacttgcc
     3541 acaactaaag ccgtgatagc cactggaact tccactaaag caagctgtcc caatgttacg
     3601 tcatatccta gatctttcag tgttgattgg actagcaacg cccctgctcc tccgatgaat
     3661 agtacttggc caaagaacca gcagacattt tccatagctg aagccatacc ttttagttct
     3721 tcttcatgct taggatggac ttctaaccct tttgattcga tggtccccaa tgccatcggt
     3781 aaaatgattg gacgaacaaa tccagcaact ccaccaaaac tcacgttaaa agccgcaaag
     3841 atcattcgaa agatcccata aatatctaca atcataccag cagatacttt cttgaatcgc
     3901 ttgatcaagg aagccgctga ttctttcaga ccatttcttt caagcgttcc tgttgccaac
     3961 ataatgatga taaaaatcgc catcccgcga ttgtttacaa aacttgtacc taaaagttct
     4021 aacattcctt gaatgccaag accgccagtc aaagctgtaa caactaaagc aaccattacg
     4081 atcaatatcg agtctaattt aaatgcaaac ccgagaataa caatcaagac gcctaaaagg
     4141 ctaagataat tttccatata ttttcctcca taaaatgaca atattttttt aattataaag
     4201 caaaacacat aaaaaatata gttgttttct cacgaaatag acactatttt taaatatttt
     4261 tcgttatttt tttatcacaa aaattatctt acttattgta aggtaaagct ttttttaatt
     4321 tattgcccga taatatatag aaataaggtc tagttactgt tttttttaaa tgattaataa
     4381 ataacaactt ctttctatga aattgcaaag cattcatctt cttttccgct aacgatatat
     4441 ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt gccgatatct ggataggtgt
     4501 gctatactac agaaaaagat atacaatctg ccaccgggta ggataaagag gatgatcatt
     4561 ccattaggtc tttgaaggga tgattttcct ctttttgtgt atttttttat agttaaggag
     4621 gaatgtaagt atggcatgga tggaattaat catagcagga attttggaag tattctggtc
     4681 aacgatgatg aaatggagtg atggtttttc taaaatcaat tattcttttt acacagtgat
     4741 tggcatgatt gctagttttt actttttgtc aaaggcaatc aaaagtttgc cgatgagtct
     4801 ctcttatcct atctggacag ggattggcgc agttggttcg gtcataattg gtgtggtctt
     4861 gtttcatgat aagctgaatc tttccacttg gttctttgtg gggatgctac tcatcagtat
     4921 cattggcatc aagattacta gcggccacta gacacttgga ataagtactg atcagaaaaa
     4981 ataagccttt ttatttaata ttcaattttt ttatgaaaaa ataagaaata agtccaatga
     5041 cctagcgact tctccactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat
     5101 atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct
     5161 cagcgatgtc agttttagct tatcccctgg caaaatcgtt ggattagtag gtcctaatgg
     5221 cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact cagatggaac
     5281 aatctgtatt ggtgaaaatg agatttctcc ctctagtcat aaagggttgg aacaagtagg
     5341 agcattgatc gaatacccgg gtatctatcc attcttaaca ggctatgatc atctgaagtt
     5401 attctctgaa accaacgatg tatctgctat cgacactatc gtgaatcagc tcaagatgga
     5461 aaagtacatt cacaaaaaag cgaaatccta ctcgttggga atgaaacaaa aattgggaat
     5521 tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt
     5581 ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac
     5641 ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat
     5701 cctgatcaat caaggaaaga tcatcaaaca atgtacaatg gctgaattga accaaacagc
     5761 gcaagatttc atcgttatct ctacaacaaa tgatctagct gctcgaacga tcttgacaga
     5821 agctggatat actttggaag atcctgataa aatcaagctt ttaaaagaaa acgaagaatt
     5881 attaggaacc gtgatccagc tattgtctgc acaaaatatc catgtgacag acgtccagca
     5941 tctgacgaat gatttggaat cttctctatt ggaaattctg gattcagtaa agggggaaga
     6001 agaaaaatga cattgcttgt aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact
     6061 gctgttttat ctgttctatt agttgttttg ctcataggaa cagcacttct agccaaaaaa
     6121 tacacgacga tcattgatcc agttgaaatg actgctcagc tttttagtgc cacatcttgg
     6181 attgtcttta tcatgattgc agctgcaagc acgatcattt cgatggaagc acaatatggt
     6241 acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt aagcaaatgg
     6301 atcacattag ttatttattc tgtttattta taccttttag ctattattgt cactgtgttg
     6361 atgaaactga ttcttttccc ttcgatttcc ttcacggaga aggtttctac tggtcaaaca
     6421 ctgatccaaa gcttgttcac ttatactttg ggtagttata tcggtttatg gctgatttta
     6481 agtttagtgt tgatgattgc ttgctttatc aatagttccg gcgcatccat ctcagcagga
     6541 atcgtctttt attttgcttc gtcaatcata tctggtatcc ttattgcact catccaaaaa
     6601 tgggagtgga tcaaatggaa tccgatcagt atgttgaatc tgcaaaacca aattggaaac
     6661 gaagagatca tgaagcaatt gactcatctt tctactaatc aaatgctatt cggtaattta
     6721 gcttacatcg ttttgttctt agcattagga tatctcgtat ttaagcgaaa aaatgtatga
     6781 tgactcatca aaaaagaggc tgtgaaagag acgttcagcc ttattgccga ttggctgtct
     6841 cttaaacacc gtttattctg aaaaaggagc tgtgacaaca tttgtgtcac agctcctttc
     6901 ctagtatgtt tttgaataaa cgacaggctc acaacctctc tttatacttc ttttatactt
     6961 ctatacgtga agcatcccag tggcaaataa aaccattgca atcactccga ccatcgtcac
     7021 acctataagt aaaatcttat ctaagtggat atgttgtaca ggctcgtttt gctccttacg
     7081 tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca agaacaagca ttacttcatt
     7141 aaaggctacg actagcatag cccatacttg aaaggcactt gctaaaagcc ctactaaaaa
     7201 ttggacccat tctttccgct gataagaaag tttcatttgg tacaatccta agaaaagcca
     7261 cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag ttataagcac tgtcagtgaa
     7321 taataatgaa agtaaaaaga cattcgtaca taacgtcgtc aatacgatag cataagttgg
     7381 tgccccattt ttgttgagtt ttccccatgc tttaggcaaa agtccgtctt cagccatgtt
     7441 tcccattgtt tcaatcggta agatcgtcca tgagagccaa acaccaacta aagaaatgat
     7501 caaaccgccg ttgatcacga ctgctcccca tttaccaacc atcatttcaa acacatagcc
     7561 catagcaggc tgcgccttga tcgtttgcag ctgattcatc gtcattgtcc catatggaag
     7621 catggaaacc aagacgtaaa taataatcaa gccgaatatc cctagaatcg ttgctttccc
     7681 aacatctgat tgtttttttg ctcgtgaacc tacgacagag gctccttcaa taccaacaaa
     7741 tacccaaagc atcaccatga tgcaactcaa tacttgactg gaaaacggga tcggctgtcc
     7801 attcgcattc aatataatgt cgctagaaaa gttatcaatg aattgttgga tcgaaaacgt
     7861 gctgatagct actacaataa ataaaaataa tggcactaat ttacagaatg tgacgatagc
     7921 attgacgaaa ctagcatttt cgactccccg attcactagc catgcatatc cccataaaaa
     7981 gactgtactc atcacgatcg atgccagatt ttgcccacct tcaaatactg ggaagaaata
     8041 tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg cctaaccagc atgtgagcca
     8101 gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa ccttcttctg cgtaactaaa
     8161 gatcccgcct tttaaatctg gacgtttttt attgatatta ttgatcgaca aggagagcat
     8221 caacattgct gttcctacga gtacccaact gaaaatagct gggccgccag ccgatccgga
     8281 gatgtccgtc ataataccaa atacgccacc tccaatcgct gaaccaatta ctaaggcagc
     8341 aagtggaatt aaaccaattc cttttttctg ttgctctgta tccatgtaag catcctccaa
     8401 aataaaaccg cttttatcaa aaagtataac aaaccttttt tatttttact taattaataa
     8461 aaactttaat agaaatataa ttctaccggc ttttttctca ttatacaagc aaagcgccta
     8521 gtccctttcc aaattacacc accaaaaaac ttaaaaaaga tgctatacta aaaggaaaga
     8581 attggaggat gatccatgaa cattttatat atatcgattt caggaaatac gcgtgccttt
     8641 gccaaacatc tagcagaata tgctgaaaaa atgcatgcag aagatcctgt caatcctgaa
     8701 gttactttaa aagaaatcca tgaaaatagt gattttacga aagaaacaga accatttttt
     8761 actttcgttc ctacttattt agacggcgga aatggtttag ataacggtga tacagaaatc
     8821 ttgaccgaaa cgatgcgtga atatctggaa catgaagaca atcataaact ttgtcttggt
     8881 gtagtcggta gcggaaataa aaacttcaat aatcagtatt gtctaacagc caaacaatat
     8941 gcacaaacat ttggctttcc tttcttggca gattatgaat tacggggaac accatctgat
     9001 gtgacacgaa tttatcagat tttagcagaa aacaataaat aataaaaaag aagaagctga
     9061 aaaatagatt tcagtttctt ctttcgtttc tattgtttct atcatattaa gctttgacat
     9121 ccatcacagg tgcttttgct tttgctttac ctgttttttc caagacaaag ttttttacac
     9181 gatccatatt ggttaccaag acgatcatgg atgtttcttt gcctgctgct ttgatcgctt
     9241 ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg ctgcccttct gttactttca
     9301 catcaaatgg cgtaccgcct aagtcaactg tgttgattcc catatgaagc aagatttcta
     9361 aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg gaagacagag ctgactaccc
     9421 cagtaactgg agaatagata tgtccgtttt ctggttctac aaagtagcct tcacccatca
     9481 tcttttgtgc aaaaactgga tcacttgctt tatctaaaga aacaactgtt ccgtcagcaa
     9541 ctgatgagag gatagtcaca ctttcagctg aagtttgagg tactactttt tcaggcactt
     9601 catttactgt ggctgtttca gtcgctaatt cttctttttc taaacggtct ttaggaatac
     9661 cgaagaagaa tgtagcaacg aagccaccag cataagctgc caataatcca aggatgtagc
     9721 ctaaccattt accgtcagag atcaatggga tcaaagcggc tcctgaaggt ccaatagcga
     9781 tagccccgac atttccgatc attccgatca ctgccccgcc gatacctcca ccgatacaag
     9841 cagtaataaa tgggcgtcct agaggtaatg ttactccgta aatcaatggt tcgccgattc
     9901 ctaggatccc gactggtaaa gctcctttga tcatttctgt taattcttta tcttttttgc
     9961 agcgaatcca taatgccaat gctgcaccca cttgtcctgc accagccatc gcaagaattg
    10021 gaagcaatag cgtacgacca gtttcatcaa tcatttgcac atggattgga gtcaaaattt
    10081 gatgcagtcc gaacataacc atcggcaaga ataatgtgcc tagaacgaag ccggcaaatg
    10141 ctccaccaac gccaagtacc cagttgatcc caccgaccat gccatcagaa atgaaacctg
    10201 ctaaaggcat gatgaagaaa atttcaatca gaccgatggc aatcaatgaa agagttggtg
    10261 taacgatgat atctaccgca tcaggtacta ctttatgcag ttttttctca acgattgaca
    10321 atagccaaac agcgaagatg acacctagga tcccaccttg tccagctgca agagcttcac
    10381 cagtaaataa gtttttcagt ggtgcttccg gattcatccc tgtcagcatc acaacggccc
    10441 cgataactcc accaagagtt ggtgttgcac caaaaacttg tgcagcattg atacctgtat
    10501 agataactag gtagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt
    10561 gccatgttgc tgcatcaagt gttccggctg tgactaagtt tgctaaaact gcggcaatcc
    10621 ctgccacgat cccagtaccg acaaatgcag gaatcattgg cacaaagata ttagaaatgt
    10681 cttttaatac cgatttgatt ttagatggtt tttgtttcga tttttgtgcc gctttcattt
    10741 cttgtgcccg ttcgtttaca cggtcttttc cagatgctga tgaatttgct gtagctgtac
    10801 ttacagaaga aagtttttct cctaatttca cgcctgccaa atcaaccatt tgctgtgcta
    10861 ctttgttcac tttcccaggt ccgatgatga cttgaagctg ttcatcttct actacaccaa
    10921 gcactcctgg aatcgctttt aattcagaga catttacaag atcatcattg atgatcttca
    10981 tacgcacacg agtcatgcag tggacaatgc tttctacatt gcccatgccg cctgcccctt
    11041 tataaatttg ttttgccagt tcttccaaag tcagttcgtt ttttgccatg gtcttttccc
    11101 cctcataagg tcccttttac aaatccgttt gcttcagcta attttgcttg agcttcctct
    11161 ttatcactat ttgtcaaaat catgacgata gccagcttaa cattttgatc tgcttcttca
    11221 aagtactttt cagctgtttc gtaatcagct tctgtcgctt gcatgatgat tcgttttgag
    11281 cgttcgatta gtttttcatt tgttggtttg acatcgacca tcaagttatt gaatacttta
    11341 cctataccaa tcatactgat agtagaaatc atattcaaga taagtttttg tgctgttcct
    11401 gacttcaatc gagtagagcc tgtcaaaaat tctggacctg catcgacttc gattgggaaa
    11461 tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa tcgttcctgt gacagcaccg
    11521 atcgattttg cgtaatctaa tccaccgatc acatatggtg tacgaccgct agctgcgata
    11581 ccaataacta tatcattttt tgtcaaattc agcttgatta gatcttcttg ccctagctca
    11641 taagaatctt cggccccttc gacagctaca gtcattgctt cttctccacc agcaattaga
    11701 cctatgacca tttccggttc tacaccgaat gtcggtacgc attcagcagc atctaacaca
    11761 cctagtcttc cacttgttcc ggctcccata taaatcagac gtccaccttt tttaaaggac
    11821 tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt ctttttcaac cgcagcggcc
    11881 actttctgat cttctttgtt cattagttga acggcttgag aaatactcat ctcatctaat
    11941 ccaaaagttt cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt
    12001 tttatcttcc ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca
    12061 atggtagctc ttctttgata actttagcta ctacattcac tttttcatcc gctggcaaat
    12121 catatttggt taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa
    12181 tagacccttt catacgcgta gctgtatttc ttgctgggat aggcggaatc tctcgaaaac
    12241 gtgcatctac gcttcgtatg acatctcttg catcgtcttg ccgattcgta tgcttcccta
    12301 atatatattc gtaaaattct tcatctacag gttccgtatg gagcaagact gtctgttctt
    12361 tttggaaaga agcaaattgc tcctgaacgt tttctgacag acctgcatct ccgatgtaaa
    12421 caatatccgt gttacttgct aatagatcta gagcgcctgc aagaggatgg atccctcgat
    12481 gttcttccaa agttggcaat ccttgatata gtgggcctct caactctgta tctcctggta
    12541 caaaaccttg gacaaggaga ccttgaattt tgagccattg attttttttc tgataccatt
    12601 ctttgtccag ccctgtttcg ggacgtggat aataattatg ccaagcttca atctgtgaga
    12661 aatcagcttc tgcttctttc aattcatcga tatctttagg cgtgatcgtg ctagcattca
    12721 aactgatttt caattgatgt gaccagtcgg caatctgctg attggaaata tgataatcca
    12781 ttcttaaacc tgttacacca attgctttca aaggcgttag atcttctaat gaaaaacccg
    12841 ctcgttccaa tgcctctcct gaaatatcga ccatcaattc tagtttattc tttttcgctt
    12901 cgcttcctag ttcgatcaat cgcttttgat atgccgaaac atcatcttct ggtatatgca
    12961 tggaagtgaa aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct
    13021 cttctgacaa agaatcgttc ataaatattg aaaagccaaa catattatcc ctctttttct
    13081 gtatccgtct tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt
    13141 ttattaaaat aattaaattt catttctaaa ggttgagttt atattatcaa aagaattgtc
    13201 ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaatatttt atttaaataa
    13261 cacaaaaaca taattactca taagaaacca attctgtatt atctaccatg aatgtaaaga
    13321 acccctcatt aggcatcgga tgaaaataag cattgatcac tcttatcaag gcattatggc
    13381 aaactaacag atagacattt tcttcgtctt cgatgcattc tttcaataaa ggaacgatcc
    13441 gtgcatacac atctaagact gactccccat ttgggaatcg tacagcgaac gctaaacgtg
    13501 ctttttgaaa gttctcatct ttgcttggca gtccatcata atccccaaaa tccatttcta
    13561 ccaatcgttc atcaacggtc agcggcaagc tgagacgatc agcaactgct tgtgctgtat
    13621 ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta
    13681 ccttttctgc tagtttctgt gcctgcatct gtcctttttc agtcagcggc acatctgccc
    13741 gtccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca
    13801 tgtgattctc tccttcgttg gcaaagttcc ccttaaccat accttgttgt tcttgagatt
    13861 acaagggtta gattctctca agagaaataa tctcagtaga tactttatcc aggaaggttt
    13921 gatcatgttc cactagtaac atggcaggtt tcacgttcaa gatcagttgt tctaattgtt
    13981 cttgattgaa gacatccaaa taattcaatg gttcatccca tgtatatagt tcagctggct
    14041 gtgataaaga tttagccaat tccacttttt tccgttggcc catactcatc tgctcgatct
    14101 tgttatgaaa aacatctctt tccatcccaa gctttcggag gttgttcaaa aatgcttggt
    14161 agtctacttg gtttttctct gcaaattccg ccaacgttcc tcgattgtct tcataatttt
    14221 ggcttgcata actaatgctc agatgttttg gctggtattt ttctcctata accttgccgt
    14281 tgaatgcccc cagaagataa tggataatgg atgactttcc tgcaccgtta ggacctgaaa
    14341 tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg ctcgaataac agattttcat
    14401 accctaattg aagatcttct acgcttaaaa gtcgcttatg gtgagacgct tggctattca
    14461 tcgtcaaaga atcgatatat tcgatatctt ttaatagttt ttctttctcg ctgatctggg
    14521 tctccatccg attcacgatt gctttggatc gtttcatcgt ccgtgcagca tcagcaccca
    14581 ctgctccttt attcactcgt ctagattcag tatcgatgaa tccgacttgt ttctttgttt
    14641 tatctccttc tcgggaacga gaccattcgg ctttttcagc tgctgttttc tttagcctac
    14701 tgacttcttt tttcaatttt tcattttgag ccatttcaaa ttcatcacga agttttttct
    14761 gttcttcata gatagagaaa ttcccttgat aaagttccag ttgacttttt tcgattgcta
    14821 aaacatggtc cactacttca tcgataaatc cccggtcatg gctgaccacg atgaagcctt
    14881 gttttttctt tttcaaataa gccgctactt gttttctacc agagatatcc aaatgattcg
    14941 ttggttcatc gattaacggg aaatgagtgt catccacaaa taaaagtgcc agtaggactt
    15001 ttgtcttctc tccccccgat agtgtgctga attctctcca taagatttca ggatctgttt
    15061 gcatcaattg gagctctctt tcgatttccc atatctcaaa atccgtaata tcatttaaca
    15121 cgtaataggt taaacgttct ttatcttttg tctgttgcgg gaaataggca aattcttgct
    15181 gatggattac ttgcccttga taaggtagtt tgttttgtag aatattcagt aaagttgtct
    15241 ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaaa tttagatttg
    15301 cttgttcaaa taataatgtg ccttggctgt cgtagccgaa tgtcagattt tttatttcga
    15361 ttttagacat tttccatcac ctcgtaaaaa aagaccagtc ctattttcag aactggctaa
    15421 agaggatgca agggttggat acacacaaaa agacccatac atcgaaaaaa ccaagccaat
    15481 cccgaaaatc tgcacactgc ttccttgtct ccacaaggta cagtctgaat gcagctttca
    15541 tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg
    15601 atagcataaa gaaaatgatt ttgtcaacta cttcgggagg atggtgaaaa atctgctaag
    15661 tgtctgtcct aagatttttg ttttttctaa tgtttgcctg tgttccattg cttcttctaa
    15721 acctattgga cccaaatgaa tacttaagac aattgggaga aaatcagaaa gtccttctag
    15781 ctcttggtct ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac
    15841 tgctacaccg tatggaactt ttccgtggat cgtttgctga tcgatccgcc cttctcctgt
    15901 aatgatgaca tctgcttgtt cgatcttttt ttccaatcca gtgatttggc tgactaaatc
    15961 gaaaccagaa accatcttcg ctcccaacaa aaaaagggct ccaccaatac cgcctgctgc
    16021 tccacttcct ggaatatcat ctaaatccac ctcccagaaa tcttttattt gctgagcaat
    16081 ctttcctgct tgtttatcta agaaagctag cgtcgtatct attcctcctt tttgtcttcc
    16141 aaattgccaa gcagctcctt gttgacctgt atatggattg gtaacatctg cagcgataat
    16201 caattttact ttagaaacaa gaacgtttct tcgatcaaaa tgaagttttt tagtagataa
    16261 taaaggattt ccttctgtgt acccttctaa agaagcaccc aaggattgga gtaatcctag
    16321 accaccatcg ctacacccgc ttcctcctaa agtgacaata atttgttcta cttcttgaag
    16381 cagtgcatct ttaataagtt ctcctaaggc ataactggaa gcaagtcgga tagtttgatc
    16441 cgttggttta tcaagtagat ctaaccccaa tgcttgtgca gattcgatga ctgcggtgtt
    16501 ttttccttgc caagttgtca ataaatagct gatttttcgc tgacggaata agagatccat
    16561 cccatcaatg gtacgccatg ttcccctgag cgcgtaatag attgcttctg cagtattttc
    16621 tccgccatct gcaatcggca cattgatttt tcttccttta cgatcttccc agccatttag
    16681 aactgcctga tttagttcaa atgaagaagc acttccttta aatgaatcaa ttgccgccaa
    16741 aatatccatc tgtatccctc actttcttct taagtaagac actttttttc atttttttca
    16801 aacaaaacaa tgctattttc ttccattttt ctatatacta aaagggaact cattgaataa
    16861 aggagagaaa aaacatgaaa atcgcaatag ctggggccgg agcaatgggc agccgaatcg
    16921 gattgatgct acatcaaagc ggaaatgagg tcctcttgat cgatcgttgg cctgctcaca
    16981 ttgaagccat tcgtacaaat gggttgattg ctgactttaa cggtaaagaa gtggtcgcaa
    17041 aactgccaat ctattcacca gaagaaatca tcgaaagcaa cgaacacgtg gacttgatcg
    17101 tagcattaac aaaagccaat caattagatg atatgttttg ttcgatccaa tccattatta
    17161 ctgacaatac ttatgttttg tgtttactaa atggattagg tcatgaagat gtactggaaa
    17221 aatatgtgcc aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg
    17281 gtcccggaaa agtcacattg ttaggtgatg gagaaatcga actagagaac ttggaaccag
    17341 aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaat ttaaatccga
    17401 tctatagcca taacgttcgt tattccattt ggcgaaaagc ttgtgtaaat ggaacattga
    17461 atggtttatg cacgattttg gattgtaata ttgctgaact aggcgcgcaa aaagcggcag
    17521 agagcatggt tcgaacaatc gtatctgaat ttgcttctat cgcagcaaaa gaaggaatca
    17581 tactagatca agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg
    17641 ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg
    17701 actacatcaa tggtgccatt tggcgaaaag gacaaaaata tgatattgct acaccatatt
    17761 gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg
    17821 ctctctgatg agaatcagct gaatcagcag ctggttctct ttttttaagc ccaataaaag
    17881 atcaaaaaga aaacacaact ctcatgatgc cataagaatt gtgcttgttt gtttcagtac
    17941 ataatctctt caaaatgtag gttttttatt tgttttcaac agaggagagg tcatattcgt
    18001 attgctcata aatagtggaa tcgttagtga taaatcgttt tgcccaattt tctgcatcat
    18061 aaaattccaa tagctctgat aggattggct taaatggtgt atgcagaaat tccggtaatt
    18121 catctaaatc acgttctcga agcaaaaaaa tattttcagg acgataaaaa tgataatgag
    18181 caatcgcttg attatccgtc actagtttct tttgaaaata catggattca aatgtccgca
    18241 aagtcactcc ttgctgaccc tcccgcaaca gttccagaat cccgcgcgat tcccctgttt
    18301 ttttcaggta ctcttcataa gtaagatatc caggttgttc attccctcga tgtggaagaa
    18361 tgaaatagtt ggtcgtaaca tctcttttta taaattcctg cttatagctt tctgctcgtt
    18421 cttttctccc attattcgtt gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa
    18481 aattcatatg tttcgaataa aatgaactat tatgtttcag cccaagttct tttgcttcca
    18541 aaaaatcaaa atgataaatc tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg
    18601 cctcgtgttc tgctttgaag tgattccaaa aaaataacac aatttttgca ttggtaaatg
    18661 ggcgcacgcg tcgagccatc tccatatcaa atccataatc taaaaaaacg atcagctgat
    18721 aatttgccaa ctgttgtcta gctgatttaa tgaattccga ctgtagtaaa tcttttccgg
    18781 accaaacatc taaagagcgg atggaaggca aaaaataaag tgatttattt tttgcaacta
    18841 gcagtacgtc atttttgttc atcttttccg cctcactttc tctgtcactt tttcctatga
    18901 tagcctgttt aaatagtaaa aagccagtaa aacgtttcct atatttgtat gaaaatttaa
    18961 catttgtatg aaaatatgat gaaataaatc attcttctat taaaattcgc ctaaaagttt
    19021 tctgaatagt tgtaattgtc tgaaaatttg ttatcattag tcattgttaa atattgctat
    19081 ttattcttaa tataaatttt gattgtgaat ttaaaagaaa gaggtcattt atggaagata
    19141 aaaaattgaa aaaacaactt agctccagac atatcaccat gttagcttta ggtggagcaa
    19201 tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg gatcgccggc ccttctgtat
    19261 tgattgcttt tttgattggt gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa
    19321 tcgttttaag cggtggcgac acgcatcacg gattatctgg tttgatacgc ccttatctag
    19381 gtgctcattc tgctgatttt actgattggg tctactggtc tatgtggatt atcaatatca
    19441 tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg gttcccgcac attccagcct
    19501 gggtatttgt ttttatgttg gctgttttaa ctacgatcat caatctttat tcggtacggt
    19561 tattcgcaga aacagaatat tggctggctt ttgctaaaat cagtgtcatt atcttactga
    19621 tcatttttgc tacttatctt gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc
    19681 ttcagcaatt gactgaccat ggtgggttta ctcctcatgg tatgaaggga atcatcagtt
    19741 ctttgcttgt agttatctat tcttacggcg gttctgaact gattgctatc acggtaagcg
    19801 aagcagatga tcctaaacgt gcgattccta aagcaatcaa aggtgtcatg ggaagaatca
    19861 tttcctttta catcattcct ttgtttttgc ttttgattct ttttccttgg aatactttag
    19921 caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag
    19981 cggatatcgt caattttgtg attgtccttg ctttgttctc atctatcaat tcgggtgtgt
    20041 atgcatcttc aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac
    20101 tagctgtttt aaacaagcat caagtaccgc aacgtgctgt tttattttgt accagtgttc
    20161 tttatcttgg tgtagctctt tcttattttg tcggtgacaa attatttggc tatctggcag
    20221 gttccctttc gtatacagta ttattgatct ggatcattat cagtgcggca tcctttgtac
    20281 tggcactaag aaaaggaaca ttatgggata aaggaatcag cttttttgca ttagctgtct
    20341 taggattgat tttccttggt atcctgttca cgaactccat tggggtaact gtattgactg
    20401 gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga
    20461 cgaatgaatg aaatcgatag aaaattttat tttatttatg tggaatagat ggagctattg
    20521 aagaaaggag ttgtgacaaa agtcttgttg caactccttt ttccgaataa acagtctcat
    20581 gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg
    20641 ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt tgttttaaat gaaaaagtat
    20701 tggaagtttt tgttgatgat cggtgtttca acggtagtta tgtttggtat gatgtacttg
    20761 aatgtctatg cgctcgatca tatcttcttc agtcgtacac gagtattcat ggcgttgatg
    20821 atgggtgcag tcatggcagt catcatgctg ttgttcatgt ggaaaatgta tgaaaacaaa
    20881 aagataaatg ttatgatctt agccgctagt cttcttgttt ttgccggttc cttgtttatg
    20941 gtacgaagcc aaactactgt caacgacaca gcttggatga aggccatgat tcctcatcat
    21001 tctatcgcta tcctggcaag taaacgagct gatttaaaag atcctgaagt aaaaaaatta
    21061 gcggaagaaa tcattcaggc acaagaagaa gagatcgaca aaatgaaaaa aatgatcaaa
    21121 gaaatggaat gaccattatc cgtccaaata aaaaaagagg atgttccaga agttatcagc
    21181 ttcaagaaat aagaagaagc tacttctgga acgccgtgaa taaggggttg tgaaaaagct
    21241 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcctatt tttcgtttgg
    21301 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtaaaatc
    21361 agcgttctga cctgagtatt agagagacaa atggaaaaat agttcctcat attttgccat
    21421 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcgagag gttgtgaaaa
    21481 agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat ggctttccac atttttgtat
    21541 aaatttggct taataccgag aaactggctt ttgaataccg tttattcgta aaagggattg
    21601 tgacaaaagt cttgtcacaa tcccttttta tgtttttatt gttctaattg cgctgtcttg
    21661 cttactgctt cttgcggttt cacaaatttt tttgagattc cccaacctgt gaaggcactg
    21721 accatcgaaa acacaggaga aagcaaactg aaaaatacga atggcaagta agtgagtgta
    21781 ggaataccaa atgtacttgc tacgaaactt ccggcaattc cccaaggcac taagtagttg
    21841 attactgttc caccatcttc tagcgttcta cctaatacag ttggatcaaa tcctgctttt
    21901 tcataagttt ctttaaatgc attaccaggc agaataacag ataggaattg ttcaccaata
    21961 aaaatattta ctccgatact tgtcatcaat gttgtaaaaa tcaaatttgg tgtgttcgtc
    22021 atctttcttg atactcgttc catcacggtt gagatcaagc caaattcgat caacaatcca
    22081 cctaatgaca aagttagaat aatcaatgac acagtaggca tcatactttc gattcctccg
    22141 cggctaagca gtgaatcgat ttgttggttt ccagtatttg aaacaaaccc tgattcgatg
    22201 accccagcca acttcgttaa ggaagtttgt gggttttgaa taaaaatcat tcctactgct
    22261 acagcgatat tcaacaaaat cgttaaaatt gccggcacct ttttccatgc acacagtaac
    22321 attaaaaata aaggaatcag tgaccagatt gaaatagtaa agtttgcttc tagaacatca
    22381 gtcacttcac gaatagatga aagacttgcg gctttacttg tgtgtcctaa aatcatgaat
    22441 aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca tcatatgctt gatgtgtttg
    22501 aacaaatcgg catcaacaat agctgctgct agatttgttg attcggataa tggagacatc
    22561 ttatctccaa aaacggcacc tgaaacaata gctccaacaa ccagcgctgg atttaagcct
    22621 agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg acataacagt aaaggcactc
    22681 cctacagcgc tacctacgat tgcacacaca acaaatacag aagggacaaa ccactggaca
    22741 ctgatcaatt tgaacccaat gaccattaga gttggaatga tccctgcttg tatccataca
    22801 gcaatcaatg aaccgactaa aataaaaata aagattggga tgattcctgg tttgatgcca
    22861 ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt ttatcactgc atacaacatg
    22921 accattccga tcgctgctaa gatcgtgata ttaggtgaga cctttaatcc gacaacgcca
    22981 atcgcaatgc ctaaaacaat aaccattaat acaagtgttg cttctaaaaa acttggtgtt
    23041 cctttttttc ccatgataat ctctcctttt ttagttgttc ttatgttcta aaaaagttaa
    23101 gatatcggat tactccgccg caattcatcc gcgattcctc ctttataaaa ataaaaagac
    23161 ctgttttaaa ctaaggacga atgactccgc ggtaccacct taattaaaaa cagatctgtt
    23221 tttcactttc aaacttttat tcaaatgtat ggactttcca tgtaattcgt caattaggct
    23281 tgatcggttc acagcgacca ccgactttct gaacaaaaca cctaaccgct actgcgtgaa
    23341 aagttgactt gtacgaagtt taccttccat aagatcattt gtcaatgaaa aagaaaatat
    23401 tttttcattt ttctgaaaat tatttttata actttcacac acaagattat tttttactag
    23461 aaagtagcgc cctcttgttt ttttcttgct actatttctt gatgttcttg cttgattctt
    23521 tctaatagct ttggctcttt caacagatca agagctgtat tggcgagtag ttctgctgca
    23581 atcgcaatag aagcaaggcc tttgtcactt ttagcagcag ctttgaattc ttctgtgtgt
    23641 ccggcaatat atgtctcaga gattgctacc gtaggctgga ttgttggtat gacttggctg
    23701 acatttccta catcagaaga accgagactt gttctttgtt ccgtttctat ttcttcatct
    23761 ggaacacctg cttcttttgc atgctggaag aataagtcat caaaagaagg agtaacgatg
    23821 acatcatcaa ctgcattttg gaacaatcca aactcaaaag tcgtgcccgt acttatcgca
    23881 gcacctcgta cgatattttc cactttttca tatactttat ctaatgtttg ccgatttcct
    23941 gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga cgttggcagc tactcctccg
    24001 tctgtaatga tcccatggat attgacatct tttggcaatt gcaagcgtag agcgttgatt
    24061 ccattaaaca cttggatcaa tgcttccaaa gcatttactc ctttttcagg tgctgctgct
    24121 gcatgggaag cgacaccgta aaatttgatg tcgactggat cattcgctaa agattctgtc
    24181 gttttcccat aacgaaaagc tggatggacg cataacgcta catcgacatc ctcaaaaaaa
    24241 ccttctcgaa caaagctgcc tttagctgat ccgttctcgc ccccttcttc ccctggggtg
    24301 ccatatacac ggatttctcc accagtttca tcaatgaatt gcttcactat gctggcggct
    24361 aatacggagt aagtaccaaa caaattatgc ccgcatgcat gaccaatacc tggtaaagca
    24421 tcgaattctg ccaaaaaggc aattgtggga ccgggtttat tggaaatata acgtgcatca
    24481 aaggctgttc ggtgtccagc tacttctttt tctacccaaa acctttcttt ttctaattgt
    24541 ttaatcaacg ctgctgagga ataaaactca tagttactta cttcaggatg ttcgtggata
    24601 tctagagcaa gtgcttgata ttctggtaaa cgatattgga ttagatcctt caattggttt
    24661 gttgagattg ttgttgtcat aaaaaattcc tcctatgcta gtcatttttc ttattttctc
    24721 ccgaatgttt cccatgcagg cacgcttgat ccttttgatg ttttctcgat tactttgact
    24781 gtgtcttctg tttgataagc gtctactatt ttttgatacg ttttattgtt ttcatcttct
    24841 tttcttgcaa cgatgatatt cacataagga cgtgcatggt catccactgg ttctaagaag
    24901 atggcatcag aagcaggatt aaatccagca tctacagcta caccgctatt gatcactgaa
    24961 atgtctacat ctgacaaagc acgtgctgtt tgggaagcat ctaattcaga aatgtctaga
    25021 ttttttctat tctcggtaat atcagaaaca gtgggcgctt gtccttttgc cttatccact
    25081 ttgatcaatc cagcagtttg caataacaga agagctctgc cgccatttgt aacatcatta
    25141 gggattgcta cttttgctcc gtctttgata tcagctacat ccttcaaagt gttagaataa
    25201 attcccagcg gtgcattgac agtatttccg atcgatacta aatcagttcc gtgttcttta
    25261 ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca aatcaatcga tccatctgct
    25321 aaagccacat ttggttgatt gtaatctgta aatttcacgt attccaaatt aatgttctcc
    25381 tttttcaaac ggtctttcac gttgtcccat acatctgtat cttctccaat aatccctagt
    25441 tttacgatag tttctttttt gtcagttgta tctgcttttc cttggctaca ccctgccaac
    25501 caaataattg ctacaaatgt aatgactaca aaacgaataa tttttttcat tttttctcct
    25561 ccactttcaa tgtgtaattt ttttaatcag ttgattactg ataaattgac ttaaaaaaac
    25621 taagattaga atcaataatg tagccacaat cgttacatct gtttggaatc gattataccc
    25681 tctcgatata gcaagattcc ctaatccgcc tgcacctact gctccagcca tggtagtcag
    25741 tccgatcacg ttgatgatag tcaacgccga gacccgaatg ataccaggca aaccttctaa
    25801 caaatacaca cgtaaaatga taccgattgg actggttccc atcgcttctg ccgcttcgat
    25861 caccccagga tcaacttcta gcagtgcgtt ctcgatttga cgagcaaaaa acggaacaac
    25921 tccaaaaatc aatggcacta aagctgcttt cgcaccaatc gtcgttccaa ctaagaaacg
    25981 agtagtcaat gctaaaagag aaagcaagat aatgaaaggg atggaacgaa tgatattgat
    26041 cagcttatca agtatttcaa atagcagacg gttagctagt aatccattag ggcgtgtcac
    26101 tactaacgtc acacctaaga tgattccaat catcccgcca atgaatgctg tccaaaaaga
    26161 catatatagt gtttgcttcg tcgcttcgac aaattcatcc ggaatcaaat aaacattggg
    26221 caaatatgtt ttaaagaaat cagtcatttt atcttcctcc ttcgatcact ttgatacgac
    26281 tatctaaatc gtttttttgt aaaatattta tttttacacc agatttgatc aaataatcca
    26341 atgctttttt ccgctgtttg gtttctccag ctaaagtaac gatcaaactc cctaacggcg
    26401 tttcttgtaa taactctaca ttgccatata aaatattcgc tgttacttga tatttgctgt
    26461 acagatgagc gattagaggt tcgttcgtct gatttccaat ataacttaat tccaccagcc
    26521 atttattggc gatctgatcg gcaaaagcag aatgggcaat gatcgtttct aatgcttggt
    26581 caagatgagt agctgttcga ataaaatctt tggtcaattc ttcttcagga ttgctgaaga
    26641 tctggatgct gctacctttt tcaacgattc ttccatcttc cataaccgct accttgttgc
    26701 agatttcttt gacaacttgc atctcatgag tgatcaggac gattgttaaa cctaactggc
    26761 gattcaattt ttttaacaat gcaaggatct gaagggtcgt tttcggatca agtgcactgg
    26821 ttgcttcatc acataataat accttaggtt cattcgccaa agctcgtgcg attgctactc
    26881 gttgtttttg tccccccgac agttgtttag gataagcaga tgctttttct tccaaaccga
    26941 caagttccag tagttcattc actttttgac gacgttcctg tttcgatttt ccagaatact
    27001 ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc cattagattg aaatgctgaa
    27061 agatcatacc gatggccttt ctttcttttc gcaattcttt gggagagagc tgactcagat
    27121 tcgtctgatt gacaatgact tctcctgctg ttggtttctg cagcaaattc atcacacgaa
    27181 ctaacgtact tttccctgca cctgagtaac cgacgattcc ataaacatcg cctttttcaa
    27241 tcgttaaatc tacagaatct actgcctgta tctctttttc tttttgtttg aaacatacag
    27301 aaacatctcg caattcaatc attaaaattc ctccttatct aaaaaaagcg tcctcaagaa
    27361 aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt taccacctca tgttcaagct
    27421 gtgttcacac acaactcctc ttcaagtacg atcatactct agcaagatat cggtcgcagc
    27481 cgtcatcgcc ttacatctat gctcgggatg aattgctcga tggccatttt ccaatagtcg
    27541 ttccttgctt cttctcagct accgaagctc tctgtcaaag gttcttctat cgtacttacc
    27601 atttcaatgc aaataatatt caaaaaaaaa gtccttttac taacataaac tatgtcagta
    27661 aaaggacgac gtatcgcggt accacctttt ttcggaagtt tccttccctc aagcccaaca
    27721 tctcaaaaag agagattggc gttcgtttta acggtcgaac agttccgaat ttgcctactt
    27781 atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct tctcaccagc
    27841 cagagcctct ctgcaaagtt tccaaactta ctttcctttt caacgaaatc gatattttgt
    27901 tgttgggatt aatctacatg aggaaaactg aaaagtcaag tacgaaattc aaattatttt
    27961 ttctatattt atttttctgt ttttttattt aacatttcaa taacagttcg aagatctttt
    28021 ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg tataatttcg gacaaaactg
    28081 gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc tcctactaaa
    28141 gaatcttcca ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa agctgtcaga
    28201 taaggaatcg gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag
    28261 taatcagcta atttaaagtg atccaagata tctgttgctc ttttcgtaac agtggtcgta
    28321 gcaatgccta gtttcatatg acgttctttt aagtctgcca atatttcttt tgcatatggt
    28381 tttaagtgga tggctccgct tgtcagctgt tgataaaaat attcttcacg cttcgctcgg
    28441 acttcctgta ctttttcagg gctacctgct gctttcacta aaatatcaaa actttgtttc
    28501 cagttttttc cgccccaagc gtcaacgatt tcttttggga tatggatatc aaattgctga
    28561 aatccccaaa gccagccatc acgatacgtt ttttctgtgt caactaacaa accatctaaa
    28621 tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca catagtttag
    28681 cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct tctcgtttat aagagttctt
    28741 ggaatcgacg gatatagatt ttcttcacag ctgtcacaag cgccagataa gcgatgactg
    28801 tagcaaataa ccataaccag taattgccag gaagtggcat caagcctaat tcagcaccaa
    28861 aacttgtaaa aggaaggaca gaaccgacaa taattcctaa tgtagtgata acaaacatgg
    28921 caaatgttgc gttgctttgg ataaatggta ctttcggtgt acgcagtgca tggagcacta
    28981 aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg gaataatgcg atgaaaatca
    29041 ctcgctgtga actatccaat gtatggaaac ttccgcccac tacagcagga caaataacaa
    29101 aatacatcaa caagtaagta gtaatatcaa atatcgaact tgttggacca aagtaaacca
    29161 tgaattcccc aatactagat gcattccatt tttttggctc gtccaaatat tctttatcca
    29221 tccgatccca tggcaaggaa atgcaggaaa catcatagat cagattcaaa aacaaaagtt
    29281 gtaaaggcag cattggtaaa aatggcagga acgtacttgc aatcagcacg gaaaacatat
    29341 tgccaaaatt agaacttgct gtagccttga tatatttcat gatattccca aaagtttctc
    29401 gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg
    29461 cagattcttt cgcaatatcc acagctgtat ctacagaaat accgatatca gccactttca
    29521 ttgcaggagc gtcattgata ccatctccta aaaatccgac agtatgacca tttttacgta
    29581 aagctgtcgt caaacgtgct ttttgctgag gattcaattt tacgaatact tcatggtctt
    29641 cagctacttg tgctaattca tgatcactca tttccgagat tttctcaccg gtaaccaatt
    29701 cattgatctc tagacctact tgacggcaca ctgaacgtgt aactaattcg ttgtccccag
    29761 tcagaacttt tacagctaca ccatgttctt tcagagcttt caacgcttgt ttcgttgttt
    29821 cttttggtgg atcaaggaaa gcaagataac caatcaaaac catgtctgac tcatctttga
    29881 ctgaaaattc tccaacgacg cttggattgg tcttttgtgc gacaccaatc actcgtaagc
    29941 catcttcatt aagtgactgt acttttgcaa gtacggcttc ttttcgttct tttgttaatg
    30001 gtgtaacagt accgccaaga tcaataaatt tagaaatgtt cagcatttct tcaatcgcac
    30061 cttttgtgat catttgtgtt ttaccagctg gatcagcaac cacaacactc atacgacgac
    30121 gttcaaaatc aaaaggaatt tcatccactt tttgataatt gatcttatct gtatctaaag
    30181 tttctgttgc ttcatcaatg atagccacat ctaacaaatt tttcagccct gtttgatagt
    30241 agctatttag atacgcatgg cgcaaaacac gatcatcttc atttccttca caatccatat
    30301 gatattcaag aatgatttta tcttgtgtca atgtccccgt tttatccgta cataacacat
    30361 cgattgcacc aaagttctgg atggcattca aatttttgat cactgttcct tttttggcca
    30421 tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt tcaggtgtta
    30481 gaccaactgc tacagatagt ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc
    30541 cattgatgac gataactgct ggagccatca atgacataaa tttgatcagt agcatggatg
    30601 ttttatggat gcctaattca aagcttgatt gaattggttt ttcagatagc gtttctgcta
    30661 catgaccaaa tagtgtttca tttccaacag aaacaatgat tccttccgca cttccactga
    30721 tgacattact tcccataaag acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt
    30781 cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct gcttgggaaa
    30841 tgaataaatc ctttgcggac aataaacgca tatccgctgg tatcatatcc cctgctgaaa
    30901 gccgaacaag atctccacag acgatttctt ccataggaat ctctgtgtac tttccattac
    30961 gttttacagc tgccgttact ttcacaagtg atttcagttt ttcagccgct tgattggaac
    31021 gtacggactg aatcaacgtc attgtcccgc tgacgataac catagtgaag ataatggcta
    31081 caccgaaaag atctttatct ccaggagctg ctaatacata ttccgtgatg aatgaaataa
    31141 ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta tgctttcaat atttctacaa
    31201 tgaatggtgt ttttttgcca tatgaaatcg cattttcacc gtaattttct cttgaacgtt
    31261 cgatttgttc ttctgtcaat ccatttgctg atgcatggaa tagttcaaat aattcttgtt
    31321 tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt tccatctcaa
    31381 tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta gctattcatc
    31441 ggatcgatag agaaaaaact gaaaggggct gagaacagat agataatagc cctggccttt
    31501 cctctagatc cttgtttttc gaatgactgt catccatttg ttctcatctc ctttactaag
    31561 taattttaat tcatgacttc caatcatttt tcgaatagtt atttttccaa ttagaccacc
    31621 tcctttttat ttttcaccga tggggatttg cggcaacaaa aaaacctctc gccaaatact
    31681 cagcgaaagg tttgaaaaag catattaaaa aaagcttctc agatctcatc gttgagtttt
    31741 gacactgtac aacgtaaaaa gcagatacct gcttttagct accttatgaa aagccttatc
    31801 tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt agcctatgtt
    31861 tgtataggag cctcacctaa caaatcttat tcggttttcc ttcgttaata taacaactaa
    31921 aaaagaggat gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa tacacacgtt
    31981 atattcctgt ctcacgcggt tttatttgtc tcattttcca cctcccgcta taatagaaat
    32041 atagtaggaa acgaggtgct gttatggaaa gaacacttgt tattatcaag ccagacggcg
    32101 tgagacgcca tttagtcgga agcatcatcc aacgttttga agcaaaggga ctggccattg
    32161 ctgaaatgaa gtttgaaact atgactcctg aattagcaaa agaacattat cagcacttga
    32221 ctgagcgcag tttttttgat gaactgatcg attacatgac ttccggtcct gtcgtttatc
    32281 ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag
    32341 ctgccgatgc tgttccaggt acgatccgag gagactacgc gctgccgggg actgaaaaca
    32401 tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc
    32461 cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc taaactgctg
    32521 gtctagttgc tgggttcagc aatggccagc agttttttct ctaaggacct ccgttgattc
    32581 tttcctaaca tatattcgag cactgtgaag aattcttggt agtctttcgt cttttcttcc
    32641 cctaaagcag cagaaatcaa tctttgataa taatctgcca tcaatgtttc atagatcact
    32701 gtacttcttt ctttatgtgc taagctccgt ttcatatctt cattcacact acgtgccttt
    32761 ttcaaccgac cattttctat ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac
    32821 tgctcatgat tgttcaactg tgctaatcgt cgcattcttt ttttgatttt caaataattg
    32881 ttctcaatga accgatcact gaatatccaa ggaaaatcag ctagaataga tagttcaaat
    32941 tgaccatagt ctggtatctg catcaagtat tcttcaatga ttcggcattc ataacgtaaa
    33001 aattcaaatt ctgtctcaga tacttctgtt ttttcgacca tgtgaagatt caaaaccaaa
    33061 taatacagat cttctgtttt cttaaaatgc ctacggttat tgctgatctc tttataagga
    33121 tcaaagctgt cttcttgtac ccgatctagc attcgtcttt ttttctttaa ctttattggt
    33181 tcatccagct ggtttcgcat ccattcatat tctgcaagtg tcatgttcat tcgctctaaa
    33241 aatgatctca atgttacaaa tgacattttt ttcactgaat ttgtctcaat cccgactaga
    33301 gaagtcttaa aacagattcc ttccgctaat tgggactgtg tcaacttctt gttgactcga
    33361 agtttcttga tcaattcacc atcatacata atagttctcc tttttaattt aaatttttcc
    33421 acattcaata aatagatacg ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt
    33481 ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact gaaggttggt
    33541 ttgattgttt attgattatt tttttgctgt tcaacgtctg ttcttgttac tggatataag
    33601 ttggttgttg ttttattacc attttttgaa aagacacttg tcgaatgttc gcctttttgc
    33661 ttttcgattg cttcattacg ttccgcttca tttttatagt catatttttt cggatcaatc
    33721 gtctcaaatc cgtttggcac ataaaaccgc aagagatttt cctgattcag cttatctgaa
    33781 agacttaacg cagtcttgac tttttcctga tctttggaga tttcctcatc gattcctgct
    33841 gctttcgtat cgatgatttc tcctgtctga ttattgtaag cttttcctcc taggacggtg
    33901 tatttgggtg tcacaaagtt cccatttcga aaagcaacta cttgatcatg ctgagaagat
    33961 aataagtctg taccaaaatg aagataatct ttatcatcga ttcccaatag atgtagtaat
    34021 gtcggcaaaa catcgatttc tccaccgtat tcatgattga ctgtcccttt agtataacca
    34081 ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc atcccatgta
    34141 tctggatctt taccaagagc gcttgctaaa tctttattat ctgtattcga gatcccaaaa
    34201 tgatccccgt aaatcacaaa gattgaattt tggtaaatac cagatttttt caaatacgta
    34261 aaaaattgtt ccaatgattg atccagataa tgtgccgttc gaacataatt atctactgtg
    34321 ctattgccag tattcaaaga tggaaagtcg aaattcttgt catccgtata ataaggcgtg
    34381 tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt gtagatgctc aagatattgg
    34441 gcagattctc tgaataaatc tttatctaat atcccataac ctaacgtttc gtcggattca
    34501 tcaaaataag atcgatcaaa gaaattgtca taacctaagt ttttatatac atgatctcta
    34561 ttccaaaaac tagctacatt cccatggaaa acagcgcttg tatagccttg cttttgtcca
    34621 aggatcgctg gtgcggcttg aaagacattg tctgacccaa gttctgtgaa taatgagcct
    34681 tgaggtaaac caaatgtgcc agtttccaac atattttcag catcacttgt ctttccctgt
    34741 cctacttcat gaaagaaatt gtcaaagctg atcgttgctt gattctggaa aatactattt
    34801 aaaaacggtg tgacttcttg tccgtctacc ttcatattaa tcaaaaactg ctggaaactt
    34861 tctaaatgga gaacgatcac atttttccct ttagcgatcc caaatgtttc gggatttggc
    34921 tctgcataat gtttctttgt ataatctaaa acttcatcaa tcccattact gcttgcatgc
    34981 gcgcgaacac tgctagtcat tcctgttttg attccgtcat aaatagtata cgcatcgatt
    35041 cctagatatt tgacaatata tgaacggtca aatgttcgct gcagcaattg tggacggttg
    35101 gcttcgctca gcgctagatt tcctgaaaaa ataagccctg aaagacaggt aacagcgata
    35161 gccatcggtt tgccgacagg gttgcttttg ataggtattt tcttgacctt caaccaaacc
    35221 agtagcccaa tgaacacagc gatatccagc cagtagatga catcatgggg cttcatcaaa
    35281 gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa agcctaatac agatttgact
    35341 gtaatgaaat ctgtgaattc ccgataaaaa ataatattca agtacaacaa aagcgtattt
    35401 aaaatatcaa tgatcaaaag aatagctaac gctggctttg gctttttaat atacaaagaa
    35461 aggccaaaaa acaataaggt agtcgcaatt gggttgatcc acaagataaa gtactggatc
    35521 gtaccttcca cacctaatga aaaatctgca tagtaggcaa tgatcgtctt cagccagaaa
    35581 aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt aagttgtgtc
    35641 acgtatatca cttctctcta ttcacatttt ttcttatttt gctcctgaaa aatgaaaatt
    35701 tcctaaaaaa gttgttatct taagattttt ttattctttc taaagattat tccgtccact
    35761 tttcacagca taaaaccgct gcgatgtaag cgattaaact aatgaagaga taaaagtctt
    35821 gtggtgaccg cttcactcca cggtattcaa aagccagttt ctcggtattc gtacaaaaaa
    35881 agaggctgtg acaaaattct tgtcgcagct ccttatttct tgatgcagga cagctttttc
    35941 acaacttctt ccttaaacgg tgttctcctg ccaactcctc ggccacaagt cacaatattt
    36001 ataaaacatg agaagctgtt ttttgaaatt gttccttgtg gctcagagtt aaacggcagg
    36061 ctcaagacct cttgatacac ggtgttcaat cagctgaccc tcggtattag attatgtcag
    36121 gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
    36181 gcccaaccaa acgaaaaata taaggagcta ttttttgaaa ttgctccttg tggctcagag
    36241 ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac cttcggtatt
    36301 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
    36361 tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg
    36421 tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc
    36481 actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt
    36541 tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt
    36601 gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc
    36661 gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag
    36721 agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg
    36781 caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca
    36841 ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc
    36901 tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag
    36961 cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc
    37021 ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa
    37081 aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag
    37141 aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat
    37201 acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg tgctaagtct
    37261 actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt atccatacat
    37321 gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg gacatatttc
    37381 agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata
    37441 aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca
    37501 taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg
    37561 cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt
    37621 ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa
    37681 ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc tgcatggatc
    37741 acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt
    37801 acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca
    37861 tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata
    37921 acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc caacgcttca
    37981 tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat
    38041 aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc
    38101 gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg
    38161 ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca
    38221 aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct
    38281 tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat agatgttcga
    38341 aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact
    38401 tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt tttgtaccaa
    38461 taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca
    38521 cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct
    38581 ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca tcacaatcac
    38641 tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg ctgctcgttc
    38701 cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca gatcgttttt
    38761 atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac cttcttgatt
    38821 gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag cagttgcccc
    38881 tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa
    38941 gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg ctaaacgttc
    39001 tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc
    39061 accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat
    39121 acctgctggt ccttctggac aattcaatgc gataactgga atactcattt ttgtgagcat
    39181 ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt
    39241 catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa
    39301 taagctatac ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa
    39361 acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc
    39421 tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat
    39481 gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc
    39541 agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc agttacattt
    39601 gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt gttgcttgtc
    39661 atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac
    39721 ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca
    39781 cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat tttattctaa
    39841 aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac
    39901 attccaatgg tataatttct ttatcctatc aaatggggga agtattttga agaaattaat
    39961 cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt tgtttggcgg
    40021 ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga
    40081 taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca
    40141 gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac aaggaactga
    40201 ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca ctaccaaaga
    40261 tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa
    40321 aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct
    40381 gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg
    40441 taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa
    40501 atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac
    40561 tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta
    40621 gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact
    40681 cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac
    40741 aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata
    40801 gaaaggctta ttctacatca cgtag
//
LOCUS       DAZKAY010000013        39703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000013 DAZKAY010000000
VERSION     DAZKAY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.013"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..629)
                     /locus_tag="ACG8WP_000635"
     CDS             complement(84..629)
                     /locus_tag="ACG8WP_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9026970.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(641..1333)
                     /locus_tag="ACG8WP_000636"
     CDS             complement(641..1333)
                     /locus_tag="ACG8WP_000636"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9026971.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1352..1636)
                     /gene="macP"
                     /locus_tag="ACG8WP_000637"
     CDS             complement(1352..1636)
                     /gene="macP"
                     /locus_tag="ACG8WP_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9026972.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1649..2212)
                     /locus_tag="ACG8WP_000638"
     CDS             complement(1649..2212)
                     /locus_tag="ACG8WP_000638"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9026973.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2440..3102
                     /locus_tag="ACG8WP_000639"
     CDS             2440..3102
                     /locus_tag="ACG8WP_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9026974.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3226..4422
                     /locus_tag="ACG8WP_000640"
     CDS             3226..4422
                     /locus_tag="ACG8WP_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9026975.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4523..5899)
                     /locus_tag="ACG8WP_000641"
     CDS             complement(4523..5899)
                     /locus_tag="ACG8WP_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9026976.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5936..6802)
                     /locus_tag="ACG8WP_000642"
     CDS             complement(5936..6802)
                     /locus_tag="ACG8WP_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9026977.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6891..7916)
                     /locus_tag="ACG8WP_000643"
     CDS             complement(6891..7916)
                     /locus_tag="ACG8WP_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9026978.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7934..8608)
                     /locus_tag="ACG8WP_000644"
     CDS             complement(7934..8608)
                     /locus_tag="ACG8WP_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9026979.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8792..9685)
                     /locus_tag="ACG8WP_000645"
     CDS             complement(8792..9685)
                     /locus_tag="ACG8WP_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9026980.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9759..11456)
                     /locus_tag="ACG8WP_000646"
     CDS             complement(9759..11456)
                     /locus_tag="ACG8WP_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9026981.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11449..12761)
                     /locus_tag="ACG8WP_000647"
                     /pseudo
     CDS             complement(11449..12761)
                     /locus_tag="ACG8WP_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(12761..16702)
                     /locus_tag="ACG8WP_000648"
     CDS             complement(12761..16702)
                     /locus_tag="ACG8WP_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9026982.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16811..17269)
                     /locus_tag="ACG8WP_000649"
     CDS             complement(16811..17269)
                     /locus_tag="ACG8WP_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9026983.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17567..18085)
                     /locus_tag="ACG8WP_000650"
     CDS             complement(17567..18085)
                     /locus_tag="ACG8WP_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9026984.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18087..18704)
                     /locus_tag="ACG8WP_000651"
     CDS             complement(18087..18704)
                     /locus_tag="ACG8WP_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9026985.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18701..19555)
                     /locus_tag="ACG8WP_000652"
     CDS             complement(18701..19555)
                     /locus_tag="ACG8WP_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026986.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19552..20463)
                     /locus_tag="ACG8WP_000653"
     CDS             complement(19552..20463)
                     /locus_tag="ACG8WP_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9026987.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20504..21838)
                     /locus_tag="ACG8WP_000654"
     CDS             complement(20504..21838)
                     /locus_tag="ACG8WP_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9026988.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22046..22876
                     /locus_tag="ACG8WP_000655"
     CDS             22046..22876
                     /locus_tag="ACG8WP_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9026989.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23120..23369)
                     /locus_tag="ACG8WP_000656"
                     /pseudo
     CDS             complement(23120..23369)
                     /locus_tag="ACG8WP_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23560..23754)
                     /locus_tag="ACG8WP_000657"
     CDS             complement(23560..23754)
                     /locus_tag="ACG8WP_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026990.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24259..24444)
                     /locus_tag="ACG8WP_000658"
     CDS             complement(24259..24444)
                     /locus_tag="ACG8WP_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026991.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25294..25635)
                     /locus_tag="ACG8WP_000659"
     CDS             complement(25294..25635)
                     /locus_tag="ACG8WP_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9026992.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25635..25865)
                     /locus_tag="ACG8WP_000660"
     CDS             complement(25635..25865)
                     /locus_tag="ACG8WP_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9026993.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26152..26490
                     /locus_tag="ACG8WP_000661"
     CDS             26152..26490
                     /locus_tag="ACG8WP_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026994.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26695..27257)
                     /locus_tag="ACG8WP_000662"
                     /pseudo
     CDS             complement(26695..27257)
                     /locus_tag="ACG8WP_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27989..28570)
                     /locus_tag="ACG8WP_000663"
     CDS             complement(27989..28570)
                     /locus_tag="ACG8WP_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9026995.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28753..29379)
                     /locus_tag="ACG8WP_000664"
     CDS             complement(28753..29379)
                     /locus_tag="ACG8WP_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9026996.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29401..30732)
                     /locus_tag="ACG8WP_000665"
     CDS             complement(29401..30732)
                     /locus_tag="ACG8WP_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9026997.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30887..31288)
                     /locus_tag="ACG8WP_000666"
     CDS             complement(30887..31288)
                     /locus_tag="ACG8WP_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9026998.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31374..31580)
                     /locus_tag="ACG8WP_000667"
     CDS             complement(31374..31580)
                     /locus_tag="ACG8WP_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9026999.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31675..33177)
                     /locus_tag="ACG8WP_000668"
     CDS             complement(31675..33177)
                     /locus_tag="ACG8WP_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9027000.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33195..33650)
                     /gene="argR"
                     /locus_tag="ACG8WP_000669"
     CDS             complement(33195..33650)
                     /gene="argR"
                     /locus_tag="ACG8WP_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9027001.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33748..34365)
                     /locus_tag="ACG8WP_000670"
     CDS             complement(33748..34365)
                     /locus_tag="ACG8WP_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9027002.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34586..35959
                     /locus_tag="ACG8WP_000671"
     CDS             34586..35959
                     /locus_tag="ACG8WP_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9027003.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36034..37146
                     /locus_tag="ACG8WP_000672"
     CDS             36034..37146
                     /locus_tag="ACG8WP_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9027004.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37218..38855)
                     /locus_tag="ACG8WP_000673"
     CDS             complement(37218..38855)
                     /locus_tag="ACG8WP_000673"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9027005.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39023..39631)
                     /locus_tag="ACG8WP_000674"
     CDS             complement(39023..39631)
                     /locus_tag="ACG8WP_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9027006.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11396 a         8444 c         6894 g        12969 t
ORIGIN      
        1 aaagagcata aaacaaagtc gtttgtttta tgctcttctt ttattatttt gtgcttttta
       61 ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt
      121 ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggaatcttt gaaaatatgg
      181 agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat
      241 acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt
      301 ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag
      361 tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta
      421 tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg
      481 gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt
      541 tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat
      601 gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa
      661 attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt
      721 gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga
      781 actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga
      841 aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga
      901 tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca
      961 aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa
     1021 atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt
     1081 gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt
     1141 gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga
     1201 cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg
     1261 ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa
     1321 taccgatttt catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca
     1381 cacataacat taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg
     1441 tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac
     1501 gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt
     1561 tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa
     1621 gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt
     1681 gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct
     1741 cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct
     1801 cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg
     1861 aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta
     1921 attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg
     1981 gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat
     2041 cttgttcgtc aaaagcaatg atcccgacac cgccaggatg aaataccagt tctctcttcg
     2101 aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat
     2161 agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc
     2221 ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc
     2281 ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag
     2341 tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag
     2401 aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg
     2461 tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt
     2521 ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg
     2581 aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa
     2641 aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa
     2701 aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat
     2761 ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca
     2821 aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg
     2881 acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa
     2941 ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa
     3001 aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc
     3061 ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag
     3121 aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac
     3181 attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg
     3241 actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg
     3301 ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa
     3361 gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct
     3421 aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg
     3481 gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa
     3541 gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat
     3601 cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa
     3661 caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt
     3721 tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag
     3781 ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa
     3841 ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa
     3901 actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg
     3961 gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa
     4021 atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct
     4081 acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa
     4141 gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat
     4201 tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg
     4261 gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg
     4321 cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg
     4381 gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta
     4441 ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt
     4501 cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc
     4561 caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt
     4621 gcagacgaag catcaataca atacaagtca aaatggagac gattggcaat agcggaacga
     4681 atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc
     4741 ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat
     4801 tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt
     4861 tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc
     4921 tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg
     4981 ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg
     5041 caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca
     5101 gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca
     5161 catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga
     5221 aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg
     5281 gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac
     5341 caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca
     5401 cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt
     5461 ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac
     5521 cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga
     5581 tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat
     5641 atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa
     5701 tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg
     5761 ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga
     5821 tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt
     5881 tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa
     5941 ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa
     6001 cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag
     6061 agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg
     6121 ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca
     6181 caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga
     6241 tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat
     6301 atacaccata accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca
     6361 ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc
     6421 ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat
     6481 ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta
     6541 gccctacaga aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc
     6601 aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa
     6661 cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat
     6721 tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc aacataggga
     6781 ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat
     6841 cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt
     6901 tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt
     6961 gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct
     7021 aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag
     7081 atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact
     7141 ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg
     7201 gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta
     7261 tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg
     7321 cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag
     7381 aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat
     7441 tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc
     7501 aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt
     7561 tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga
     7621 ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc
     7681 aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa
     7741 ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg
     7801 ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa
     7861 aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta
     7921 accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt
     7981 ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt
     8041 catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct
     8101 ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga
     8161 tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca
     8221 tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg
     8281 caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag
     8341 ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata
     8401 cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta atatctagta
     8461 atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc
     8521 caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt
     8581 cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta
     8641 caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa
     8701 acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa
     8761 atttattatg ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt
     8821 gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta
     8881 gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga
     8941 cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt
     9001 aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg
     9061 tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca
     9121 cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct
     9181 cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag
     9241 aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata
     9301 aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat
     9361 attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc
     9421 ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta
     9481 attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc
     9541 ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag
     9601 ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag
     9661 gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa
     9721 cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta
     9781 gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc
     9841 aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct
     9901 ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg
     9961 ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc
    10021 tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct
    10081 gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca
    10141 taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc
    10201 cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac
    10261 tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca
    10321 tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct
    10381 gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta
    10441 cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg
    10501 ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc
    10561 acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa
    10621 tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct
    10681 gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt
    10741 tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt
    10801 ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct
    10861 ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata
    10921 acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga
    10981 tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct
    11041 tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa
    11101 ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct
    11161 cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac
    11221 aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta
    11281 ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca
    11341 cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt
    11401 cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt
    11461 ttctccccgt tcttttttta ttaataaaac agcacctaac aataaaagaa ttcctagtaa
    11521 agaaatcaac gttcttgctt cattcgtttg cgggaatacg gaccattttc ctgattgacc
    11581 tggattgctt ggaccactcg gctctttcgg tttgtccgtt gtttctttta cggtctcgtt
    11641 ataaacacgt ggtgttgcgg attcataggc agagactgga actttcccgg attctaattc
    11701 agccatctcc tgtccgttaa ctactacata ttgtggttgg ccttttgcat ccacccaaaa
    11761 ttcagggatg atgacctcga ttctcttact tgcttcatcc atctcgtaac cttccacacc
    11821 ttttaattct tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag
    11881 gccctcttta tccgaaatga atttgcttac atttttgtca ttcaaaggat cattgtctgc
    11941 tggtttgatc cattgatttt tcaagtcatt agcaggagcc atatctaaat aatatttccc
    12001 ccttctatca tctgatatag agcaaaaaca gcaccttcta atggttttcc tttttcagtt
    12061 gttcctcttt gacgtccata cttgaagaag tatggatctc ttagataacc gacattttta
    12121 ggatagatat gaatctcttg cagttcctcg gttgattctg tcgggtgata aattggcaaa
    12181 atagcagcaa ttgggatagc tgtctgatcg atatcaacat ccaatccaac ttctggatca
    12241 atcgattctt cgaaaatcaa ataaactgca tcccgatctt ggtttttctt aggtgctttg
    12301 atacgtgcaa tcccgtctcc taatccgcca accgtatctt tcccagttgt taccgaagtc
    12361 cctatttgga caagcctttc ttctgtgatt agctgacgga tattgtttct gttcgtttca
    12421 gcgatttgct ggacgaattc tttaggagtc atgttttcag cagctttttt ttcatgataa
    12481 taatcggtca tgtcgtaaat ggcaaatgtc gctccattta gtggcaccgt ctgtgaaatg
    12541 atatctgctg tttcccctgc ttcctcagag atagccaagc catcattttc atggtattca
    12601 aattgatcgt tataatcgat atctcgaatc atccgtttat gcaacacgaa ttccacgaca
    12661 tcttcccctg attcttctgc cgacatcgca tgtggaagaa ggactgctgc aagcggtagt
    12721 aaaacaagta attgaagcat caaacacaac agctttttca ttaccttgct ccctcctttt
    12781 tagattgtct gacaaacaaa ccataggctg ctcctgcaag cgccatgaag aatgcagcaa
    12841 gcaggatata gaataatcgt ccggatcctc ctgtagcagg caacggattt tcaggatcgt
    12901 tcccgacact aagctgtaat ctgattcgat ttgtctccaa aagttcatat tctaactctt
    12961 gctgtagatc aggcgtctct gcttcttcgt ttggataata cgaaatctca acttttagtg
    13021 tacccgcttc ttttcctgga actacccgat cattttctat gtcaggttct tctcgataac
    13081 taatttctaa aataaagtaa cctggaagtt ttctatagct gtcaggtgac ttcacttcct
    13141 tgatccgata gaccccttcc aacaagtctg aagcattgaa tagggaagaa tcgtttggat
    13201 ctggtgccaa gcctccctcg atgatttcat actctcctgc actattttgt ttctcaattg
    13261 aaaattctgc gccttcaaga cgtcggctat tcatactgtc aattttcaaa agttccaaat
    13321 cgattggcgc tagttcatct tctaaggtta cttgtagtac accatcgatg atttcatagc
    13381 cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc ttcaatgata aacttacctt
    13441 cttcattcag tgcaaactgg aacacttgat cagatagccg atacccattt ggtgcgacag
    13501 attccgagac ctcatattca ccaatagaca gatgcagagg ttggtcgtaa tttaaatcga
    13561 catacttttc tactcctacg gcacgagaat agaagtatcc gatcccaggc aaacgtttcg
    13621 attcttctgg ttctaacgga ttaccagtgt atgctcgata cagcgggaag gaagaatccc
    13681 ccactttcct gatatcgaaa cctgcaccat ctaatgtatg gccttcatgg tctaattttc
    13741 taactcggat ctcccgataa tcaaacgtat tgtaagcagt gaatgctacc cataaacgat
    13801 cctcttcatc tgttgtatac gtgagatatt gccaattttc ttcacctgtt tgcttgagtt
    13861 ggatagtccc tatagcatta tcgtaaagtc gcaagatcca tgtgtcattt tcatcaggtt
    13921 ctttgaaacc ttctgggttc tggctctcaa tgattttgta ttctgcatag ttggtagttt
    13981 cttcactatc cttataaaca aatgaatctg gctctccgat tggatgaata actttctcac
    14041 catccacgat tacatatttc ccgtcttcat ctactgaata gaacgtgagt ctccctttct
    14101 cttcctctac ttcgttcaca ccagaaagcc ctttcgctac tggatatccg atttccagtc
    14161 cgttaccatc tgtttcataa agttcaaact ctgcgccatt gattggcata ttactgcctt
    14221 caattagttt attgatattc atgtggattg gcttgaattc atttcgcatt tccagtttga
    14281 catacaactc atcatactct gtgccttcta tgttaggctc ttgtacaaca gaaagcttgt
    14341 gatattctcc ttcataatag gaattcggat ctttaccttc cggtccgccg actctcgaac
    14401 cagatgtata ttcatctaca agctcgtagt gacctaacat ctcatcttcg ttggctgcat
    14461 attttttgat ttggaattga tacgtctcat ctactaactg ataacgtggt ggtccagcga
    14521 tctctctgat ccaaaatatg tcgctgactg ctccagtttc atctcctaaa atcggcatct
    14581 tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact tgtttcgatt ggttcggctt
    14641 ccccactctc tggattttgg tcatataatg caaattcgac tccagccaac ggctcgccat
    14701 tttgaccctt cttgctgact tggaaattac ggcctttatg ttcgtaacga atggaagaag
    14761 atgggaaata catccgtcca gtagtgatat cattctcata atcctccagg ttctgaatat
    14821 aggccatatt gttcactgtg caaaaatttc cgtcttgagc tcccgcatca agttttactt
    14881 ggtacttgat cctgaccatt tccccttcac ctaaagaaat cggagaaaat ttataagctt
    14941 ggttttcccg tccgacatac gttgtcggag tcgccactac ttctggtggg gcttgtccat
    15001 gacgttcttc atcccatatt tctggctctt ggtacttcat gccgttttct cctggatcta
    15061 gatggtaaaa ctgaatatct gccgatctgc cagctccaaa tggtgagaca ttaaaaccag
    15121 tttcatcata aagtgtggct tgctggatcg ttctcttgaa ggtagaagag atatcctcca
    15181 aggctgtaac aatgtcattc ttgttgttcg cattgtaata tcgcggtgtt acgccatctt
    15241 cttctaaatc agcaatattt ttcaatacat ttcgtccaat agcatctagt cgttgtctcc
    15301 cgctaacgtt tccatcgatt cctaaaccaa tggagaagat acttggcggg ttctcttgat
    15361 acttgttttt cagccaatga tgataagcaa ctgtgttgat cgcagatgac ttttcttgcg
    15421 aagtaggact gccattcgta ttgttattag aagaaccatc accaacccag tgagtcgctt
    15481 tattatctcc ttcttttacc tctccataac gatagtgtac atttacttca tcttctggac
    15541 ggttaaagtc agatgcatag gtaactggat gagtaaaccc tgcattagaa gtattcccag
    15601 aaaactcctc aaaatttctg aacaaaacgt cattatcttc agcaatctta tcttccatca
    15661 cagtccagtt gctccaagct ccattagtgc tcgtacgata acttgactga atcggtgcat
    15721 aactgaaggt cggtgtccca tctcccacaa cgattaggat tttttctgga tttctatttt
    15781 ctccgccatt gtctttatac aatgtttcat agccattttt aagccctagt gtaagcggag
    15841 ttcctccgat tggtgtacga tttaatgctg tattctcact tcccgtcaaa cgattgatat
    15901 catttgtcaa tggaaagatg tccgaactga tttgctcatg gttattgata gctgtactat
    15961 tatggtaaaa attcaccata ccgattcgaa tatccagact cgggtcagag agcagatctt
    16021 tactcatttc gtttactgcc tcaatcaacg cagcattttt agtttgacta ttggtcccag
    16081 cggttaattc actcatactc gctgacttat ctagaacaaa aacgatatcg attggactga
    16141 tttccttttt ctcgcccccg atgacatcta gatagacatt gtaaagggtt gttgcatctt
    16201 caggatgtag atcagatgct gtgctgcttg gcagaacagg ttctacccat tttttgatgt
    16261 acgccccgtt ataatccaag taactgtttt caaaattccg ttcatttccc caaagctgaa
    16321 cggtttgagc tggaacgttt ctatcttctt cgcctgcagt tgatccatta ttagtagcat
    16381 agtcgtaatt tcgccagttc gctcccccgt ttgtataatc actatcatcg atcaagttct
    16441 ctccgtcata tggcgagcct ttgtctcctt gatagcgttc attgctttgt gtccaatttc
    16501 ctaacacttt tgtatagtca ttcgttgtaa acttcgggta gcgatgctgt tcgttttcaa
    16561 cttctccgct aggatagata tattcatagg ggtcttgcgt agctgttggc ccaagacgct
    16621 ccaaaatacc gttgaataac tggcgttgaa tgcgctggag tgcttgaccg ctgggtacga
    16681 ttggttcgtc cagcttttcc atctcatttt cttcgctacg ttgaatttca gcggaatctt
    16741 ctgtatcagt ttcattttct tgtaattctt ggttatctat tctttcatca accaccactt
    16801 gaagaggtag ttacccttcg ttcacatcgg ggatcttttc tgtttcaaaa atcaatgtgc
    16861 cggtttctgc ttcttttgaa taaacttttc cgacatacca ttcttgacca tcgatcgtct
    16921 cgacttttag gtcttctcca tcttcatcat aggaggatag acctgttcca cgaacgtttt
    16981 ttacggtacc aatcccggcg gctgccgcat cgattgctaa acgtattcta ccttcattgg
    17041 cactttcctt tttttcaaaa gccagatgcc attcaagggt ttcttctttt tcaatatact
    17101 tcccttcaat ttttgcatag tcctcatcta agaatacctc atcctccttt gtttctgttg
    17161 actcagcagc tacatccagg gtcgtcatat gatagactga ctggaaatat ggtccaaata
    17221 gcaaactgac agttactaac tgcaaaagct tcaattttaa ccctttcaca tccttatact
    17281 cctttcaaaa atatttgtct tctttcacaa caatcaacaa aacaatgccc atctaattat
    17341 aatttaattt aattttaatt ttaaatcaaa tgatatcctt tttagttaaa aaacaccaat
    17401 tatacaacat ttattattaa aattaaatta taaaatttta taacgttctc atttaacaaa
    17461 acttaagtta ttaagacaaa tgaataaaca actacgcttt cctcgaaaca aaaaaataaa
    17521 gcacaaatgc gcatttacgc actcatgctt tattcgtaca attaattcag ataacggcag
    17581 cgcataggat gccaataaca cttcctaaaa tagctcctcc taaaatgtca gtaggataat
    17641 gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc aaataaaaag gctaatattc
    17701 ctgcaactgg acttaattcg aacaacgcag ctactgataa aaaggctagc gctgtatgtc
    17761 ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag gacaagccga cgattttttt
    17821 cataaggtct tttccgtctt gtcgtttttt tgataaaaag caaaccaaca aaaaaataga
    17881 tcaaactgcc gataagaatg gctagaccat attctctagt atcaggtatc aagactaaaa
    17941 cagcagcaag gagcaaccaa ataaaaccac cattagagat attactgata aatttcaaaa
    18001 aattgatttt tcgactatcc gttttccgtt gcttggcgaa cttgttgata atactatcat
    18061 cgattttagc aatcaaacgc aacatgttat tcctcctttt taagaacctt ttcttgcgta
    18121 cgatggataa aaagcaggac ttgaaaataa aacaagaaac tgaaacctaa taaagcaaaa
    18181 tagattggtc ctgcaaacca gaatgcgaca agaaggatca acgtgcctgc caaatagaaa
    18241 agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag taatccaaga agcttctttt
    18301 tgttccagtt gaagcaagat tttgtaacta aagagaagcg cgcttaatag aagcaaaaac
    18361 aacgtagtat acatcaatgt atagccaatt cccccacccc aatagcggaa aatctgataa
    18421 tcgatcaata gaagaagaaa ataaacactt gctaaactat cttgctttcc ataaagagag
    18481 aagttctcac gaaacttttt ccagaatagt gaaccgagct ttttctcttc attttttttg
    18541 taatgataaa tcatatacag aactgttcga cttgcagttc ctaatgtgaa aacaggcaag
    18601 acgcacagga accatagtag ctgtaaatgg ataagacgtg gaatccattc aatgatttct
    18661 aagaaataat cattggcttt tttaccaatc gatcgattca tcatcctttt gaacctcctg
    18721 tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga
    18781 tcaagacaag tgttgctcct gccatcagat aattccattg tgtcgttgct tggttctgaa
    18841 aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc ttggatatat aacagaggac
    18901 ctaagaaatc attccaagct gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt
    18961 tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg attcgctccg tcgatttcag
    19021 cagcttcgat caattcatta ttgatcgata agtaaaattg acgcatcaga aatatattga
    19081 acgcattgcc gaagaacgat ggaacgatca ggggcaaata agtacctacc cagccgatct
    19141 tggagaataa tacatattga gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa
    19201 gaaccaaagc aaacatcagc tttttcccag gaaaatcaag cttcgcaaat ccataagcaa
    19261 taaaagaatt tgaaaggaca ttaccgatca ctactagcac agtgatgaat aatgtatttc
    19321 ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag atagttttcc catacgattt
    19381 tttctgggaa aaaggtcgga ggataacgaa cgatttcttc cattggtttc aatgaagtaa
    19441 acaccatcca aagcaatgga atgatcaaaa tgattcctat tactgtcatg aggacataac
    19501 ttagtatttt ttttgtttgc cgattcacac tggtcttttt gaaataatcc atcatttgcc
    19561 gtcacctcca gaataataga cccacttagg tgcaagcttc aaattgacaa acgtcagaac
    19621 caacacgatc agaaacagga tcattgacat agccatcgca taacccatat caaaagctac
    19681 gaacgccttg ttccacatat gcagattata aaaaagcaaa gaatttgcag gtccgcctgt
    19741 tccatttttc gtcatgacat aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt
    19801 gatcacatcg taaaaaataa taggagtgat taatggcaaa gtaatacttt tgaactttcc
    19861 ccaaacgcct gctccatcga tttctgctga ttcatataaa gatctaggaa cattttgtaa
    19921 tgtagctaag taaagtagca ttgctcctcc agaactccat attttcatca aaatcaacgc
    19981 aggtttcgtc cattgtggat caaacagcca agctggtcca gggatatgga accagcccag
    20041 aaatgtgttg atcaatcctg cagatggaga aagcagctgc atccataaga tgtagactgc
    20101 tactcctgat aagactgccg gtaaatagaa agctgtacgg aatacactga ttcctttgat
    20161 tttttgattg agtactgtgg ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa
    20221 gatgacgaaa taaacggtgt tgtataacga tttccaaaat aaatcatcgt gaacgaacat
    20281 tcttttgaaa tttgctagac caatgaaatt catcttggat gtcaaattat aatcggtgaa
    20341 actaccaata actgaaaaaa gcataggccc tagcataaat accaagaacc cgataataaa
    20401 aggcgagata aataaatagg cccatcttgc ttcttttttc tttcggatag ataaacgctt
    20461 caaattttct tgcctcctct gtacctagac agtcaaaaat ggattattga ttttgcttga
    20521 ctaagttttc tactgctttt tgtgcatctg ccaatccttc ttttggtgat tttgttccta
    20581 aaagaatctg gtcaatctgt tcattcacta atgtcgtgta atccggagca tctaaaggaa
    20641 ctggtgtgat caccgtattt ttaaagtttt cgtccgccat ttgatagatc atttttcctt
    20701 tatccgttag gttgtcatta ttcactaaat tctcattggc tgtcttgctc gccatgatat
    20761 caaaactttt ttcgccgaat ttttcttgta cttctggaga agaaagatat ttgatgaatt
    20821 cataagaggc ctcaggatct tttgctccgt atggtacttc caagacgaat ccgccacccc
    20881 acgaccaatg gcctgaacct tcttcttttt ctgggagctg catcacgcca aaatcaaaat
    20941 catccggtgc attttccatc aaactagagt aataattgat attttgggca cgcatggcta
    21001 ctaatccgga aataaatgga tcagatacac cagacccaaa ttcggcttct aatttattga
    21061 tcgtattttt cccataataa tcttgccagt cttggatcca ctccaaggct tccactttat
    21121 ttttagtatc aatcttgact ttttcatctt tgtcaaacca actagtcccg ttatctgcgt
    21181 ttagcgccca tacatcagcc cctaaattcc ataatggata aaaaccgatg cgtgtaaatt
    21241 cttttccttc ttttttatct agtttacgtg ctgtttcttc taattcatcc caagttgtcg
    21301 gtagatcttg ttcactgatt ccagcttctt taaataactt tttattataa aaaatgactt
    21361 gtgtatctgt attgaatggg atcgcataag gatcaccttc atattctact gtatcccaaa
    21421 gctgcggata aaaatcctta gaagtgtctt tttctacata tttggagaga ttagttgctt
    21481 gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg tacaataaca tcgggtgcat
    21541 ttccagctgt tattgcagat aaagatttag tccagatgtc accccatggc tgatacttat
    21601 aatttacatg gatctcgttt tgtgattgat tgaaatcctc aatgatttct tcgatagctt
    21661 cttttcgtgc accggatccc caaaaggacc agaaatcaat attttttctt ccatcacttg
    21721 ttttttcaga agaatttgct gcacttgaac tggcatcgct tgcattactt gatccgcatc
    21781 ctcctaataa aaacaatgct gaacttgcca gcaaccctgt aaacaataat tttttcatat
    21841 tttacctcca aattttaatg taaccgatgc cggttttaat ataacgatat cattttgttc
    21901 tgaaaataac aatttataag gtatgattct aaaaaaatta ttaatgatac caatacttta
    21961 tataattcgc gagcgctttc tttgaatttt ttaatctttc gtagcagaat gagaatgtat
    22021 taaaaataga ttaggaggaa ttttgatgtt tagacaccag aaagaattgc agtttgaagc
    22081 aaaaccagat cgtccgaatc cattaatcgc gaaatatctg caggagctga tcggcggcca
    22141 atatggcgag atgagtgtag ctatgcagta cctgttccaa ggctggagct gccgaggaga
    22201 agaaaaatat aaagatatgc tgatggacat tgcaacagaa gaaatcggcc acgtggaaat
    22261 gttggcgaca atggttgctc gtttattaga gggtgcgcct gttgaagatc aagaagcagc
    22321 gatgcaaaaa gatccaacaa tcggtgccat tatctctgga atgaatccac agcacgcgat
    22381 cgtagctggc ttaggacctc gtcctgctga cagcgtgggc aatccatgga gcggtgccta
    22441 tatcattgcg agtggcaact tgttagcaga ctttagagct aatttaaatg ccgaatcaca
    22501 aggaagactt caagtagcac gtatttacga aatgatcgat gatcgtggcg taaaagatat
    22561 gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa
    22621 agaattagaa gaaaaagaag gttcaatctt agtcccaagt actttcccgc gtgacaaaga
    22681 acgaaaagac attgcttatg acttctataa cttctcagag ggagaccaaa gtagtgaagg
    22741 ttcttgggct agtggcaaag cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc
    22801 taagatcgaa ggcgctgcac caaaattaga accagtaaca ccaaaaatgt atgggacacc
    22861 tccattcaag aaataaatat ctacccgtag aaacacccgt tttttgttcc acacgctttg
    22921 tgagcaaaac tgactaaagt cattaatcat aaccaccctt caaaagggtg gttttctcta
    22981 gagctataag ctcaaatcac cggccagcgc ctaaagacgc tggctttcat attttatgtt
    23041 caagccatat tgcctttgcc agttttgccc acctcttaaa aggtttatgt actactcctt
    23101 agcccttaaa aggatcgtct tactctcgta cactcagctt atctaacgca ctatcatgtt
    23161 tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt ttgtcctact tttgtcacat
    23221 agtatccttc cacccaaaag tgtcgatacc caaatttata ttttagattt gcacaatctc
    23281 ttgatgatct cctgaatatt ggctcgatat tgattataaa ttatttttct tctatacttt
    23341 ggtgtgaata caatgtggta tttacacatc tactttgtac gggctaaact atttcccttt
    23401 attgctccac ccgcatagca agtggttttg tatttcaatc gctcggcgcg ctcaattagg
    23461 tctaaagaca ataaatctaa aaacccgctg cttcactgac agaaacaacg ggttttcaga
    23521 aaaaaatgaa atgaaaaaga tctcttacgg caatctattt tatttttttg attttttata
    23581 aatggaaaat gcttcaagaa gtccaagaaa taaagccgta taaaaacaag aacctatgaa
    23641 atctttattt attagatatt caacagaaat cccgataaca ctagccaaca atatatttat
    23701 cagaagtgta aaataaggat gctttttata gaatagaact agtgttgttt tcatagactc
    23761 tcctttcttc atatttccaa ttaatcacaa attcacggca ttactagaaa aattaaaggg
    23821 gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa
    23881 taactatacc aaatctaagg tacctctaaa tctaaattcc atttgtggcg gtgacctcca
    23941 atttttaact cttctatctc ccatccagta tacataacct tgatacttcg accacttata
    24001 tatcgaacac acaaatacct atccattgca ggagtactac ctaacttagt tgctacttgc
    24061 atctaaataa cagctgaaaa gataggactt actcttagta aagtatctga actattaatt
    24121 tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc actttctgtc
    24181 tcatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga cgctggaata
    24241 gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa aaaatacaat
    24301 gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca gcaactcatt
    24361 actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata agatggttag
    24421 gaatccttta gaatttttac tcattcatag acacttcctt aaataacttt tattatagaa
    24481 ggtattgtaa ataataatat tgacaaaaag tgactcaaaa taaaaaaaac cttacattat
    24541 actggaatga actgtattga tagtgtacct aaaagctcta ctgacataag atcgtgtaat
    24601 cggtaaagta attttattaa ttatacggga tgtaatcaat tagagaataa actaatcata
    24661 aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc actatctgac
    24721 ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact aaataaaaac
    24781 tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag tgatggcaaa
    24841 tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat tcagttaaac
    24901 tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg agcatcgata
    24961 tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac taaaactgtc
    25021 agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat acgttaagat
    25081 atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc
    25141 aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt aatgccatcg
    25201 accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt tatgtttagc
    25261 atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta taatctgatc
    25321 aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa
    25381 atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa
    25441 tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga ccgaaaacat
    25501 tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc
    25561 ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat
    25621 tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct
    25681 ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac
    25741 gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt
    25801 ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca
    25861 agcattatca tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc
    25921 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
    25981 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
    26041 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
    26101 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
    26161 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
    26221 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
    26281 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
    26341 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
    26401 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
    26461 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
    26521 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
    26581 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
    26641 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
    26701 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
    26761 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
    26821 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
    26881 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
    26941 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
    27001 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
    27061 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
    27121 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
    27181 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
    27241 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
    27301 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
    27361 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
    27421 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
    27481 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    27541 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
    27601 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    27661 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
    27721 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
    27781 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    27841 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
    27901 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    27961 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
    28021 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
    28081 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
    28141 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
    28201 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
    28261 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
    28321 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
    28381 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
    28441 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
    28501 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
    28561 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
    28621 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
    28681 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
    28741 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
    28801 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
    28861 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
    28921 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
    28981 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
    29041 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
    29101 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
    29161 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
    29221 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
    29281 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
    29341 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
    29401 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
    29461 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
    29521 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
    29581 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
    29641 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
    29701 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
    29761 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
    29821 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
    29881 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
    29941 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
    30001 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
    30061 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
    30121 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
    30181 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
    30241 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
    30301 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
    30361 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
    30421 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
    30481 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
    30541 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
    30601 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
    30661 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
    30721 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
    30781 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
    30841 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
    30901 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
    30961 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
    31021 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
    31081 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
    31141 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
    31201 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
    31261 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
    31321 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
    31381 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
    31441 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
    31501 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
    31561 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
    31621 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
    31681 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
    31741 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
    31801 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
    31861 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
    31921 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
    31981 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
    32041 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
    32101 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
    32161 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
    32221 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
    32281 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
    32341 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
    32401 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
    32461 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
    32521 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
    32581 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
    32641 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
    32701 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
    32761 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
    32821 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
    32881 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
    32941 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
    33001 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
    33061 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
    33121 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
    33181 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
    33241 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
    33301 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
    33361 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
    33421 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
    33481 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
    33541 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
    33601 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
    33661 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
    33721 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
    33781 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
    33841 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
    33901 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
    33961 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
    34021 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
    34081 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
    34141 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
    34201 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
    34261 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
    34321 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
    34381 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
    34441 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
    34501 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
    34561 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
    34621 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
    34681 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
    34741 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
    34801 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
    34861 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
    34921 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
    34981 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
    35041 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
    35101 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
    35161 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
    35221 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
    35281 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
    35341 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
    35401 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
    35461 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
    35521 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
    35581 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
    35641 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
    35701 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
    35761 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
    35821 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
    35881 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    35941 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    36001 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    36061 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    36121 tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    36181 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    36241 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    36301 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    36361 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    36421 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    36481 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    36541 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    36601 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    36661 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    36721 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    36781 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    36841 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    36901 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    36961 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    37021 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    37081 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    37141 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    37201 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    37261 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    37321 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    37381 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    37441 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    37501 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    37561 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    37621 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    37681 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    37741 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    37801 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    37861 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    37921 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    37981 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    38041 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    38101 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    38161 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    38221 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    38281 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    38341 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    38401 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    38461 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    38521 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    38581 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    38641 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    38701 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    38761 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    38821 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    38881 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    38941 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    39001 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    39061 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    39121 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    39181 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    39241 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    39301 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    39361 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    39421 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    39481 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    39541 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    39601 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    39661 ttctttcgac gctattaact ttatcattta gaaatatttc ata
//
LOCUS       DAZKAY010000014        39234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000014 DAZKAY010000000
VERSION     DAZKAY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.014"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACG8WP_000675"
     CDS             66..767
                     /locus_tag="ACG8WP_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9027007.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQNPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ACG8WP_000676"
     CDS             764..1885
                     /locus_tag="ACG8WP_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9027008.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8WP_000677"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8WP_000677"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9027009.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ACG8WP_000678"
     CDS             3264..4172
                     /locus_tag="ACG8WP_000678"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9027010.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ACG8WP_000679"
     CDS             4207..4479
                     /locus_tag="ACG8WP_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9027011.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ACG8WP_000680"
     CDS             4490..5536
                     /locus_tag="ACG8WP_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9027012.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8WP_000681"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8WP_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9027013.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ACG8WP_000682"
     CDS             complement(8547..9233)
                     /locus_tag="ACG8WP_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027014.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFK
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ACG8WP_000683"
     CDS             complement(9211..9705)
                     /locus_tag="ACG8WP_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9027015.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ACG8WP_000684"
     CDS             complement(9725..10945)
                     /locus_tag="ACG8WP_000684"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027016.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8WP_000685"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8WP_000685"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9027017.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..14034
                     /locus_tag="ACG8WP_000686"
     CDS             13123..14034
                     /locus_tag="ACG8WP_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027018.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15030..15281
                     /locus_tag="ACG8WP_000687"
     CDS             15030..15281
                     /locus_tag="ACG8WP_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027019.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15394..15636
                     /locus_tag="ACG8WP_000688"
                     /pseudo
     CDS             15394..15636
                     /locus_tag="ACG8WP_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15726..15965
                     /locus_tag="ACG8WP_000689"
     CDS             15726..15965
                     /locus_tag="ACG8WP_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027020.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16112..20395
                     /locus_tag="ACG8WP_000690"
     CDS             16112..20395
                     /locus_tag="ACG8WP_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFP9027021.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20494..20859
                     /locus_tag="ACG8WP_000691"
     CDS             20494..20859
                     /locus_tag="ACG8WP_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027022.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20861..21313
                     /locus_tag="ACG8WP_000692"
     CDS             20861..21313
                     /locus_tag="ACG8WP_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027023.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21762..23237
                     /locus_tag="ACG8WP_000693"
     CDS             21762..23237
                     /locus_tag="ACG8WP_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFP9027024.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23260..23571
                     /locus_tag="ACG8WP_000694"
     CDS             23260..23571
                     /locus_tag="ACG8WP_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027025.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23932..25188
                     /locus_tag="ACG8WP_000695"
     CDS             23932..25188
                     /locus_tag="ACG8WP_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9027026.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25185..25730
                     /locus_tag="ACG8WP_000696"
     CDS             25185..25730
                     /locus_tag="ACG8WP_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027027.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25731..26231
                     /locus_tag="ACG8WP_000697"
     CDS             25731..26231
                     /locus_tag="ACG8WP_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027028.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26590..26868
                     /locus_tag="ACG8WP_000698"
     CDS             26590..26868
                     /locus_tag="ACG8WP_000698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027029.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            26888..27232
                     /locus_tag="ACG8WP_000699"
     CDS             26888..27232
                     /locus_tag="ACG8WP_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027030.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27251..28162
                     /locus_tag="ACG8WP_000700"
     CDS             27251..28162
                     /locus_tag="ACG8WP_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9027031.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28241..29233
                     /locus_tag="ACG8WP_000701"
     CDS             28241..29233
                     /locus_tag="ACG8WP_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9027032.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29230..29448
                     /locus_tag="ACG8WP_000702"
     CDS             29230..29448
                     /locus_tag="ACG8WP_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFP9027033.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29461..29871
                     /locus_tag="ACG8WP_000703"
     CDS             29461..29871
                     /locus_tag="ACG8WP_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP9027034.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29923..32451
                     /locus_tag="ACG8WP_000704"
     CDS             29923..32451
                     /locus_tag="ACG8WP_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9027035.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32467..34677
                     /locus_tag="ACG8WP_000705"
     CDS             32467..34677
                     /locus_tag="ACG8WP_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9027036.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34685..35776
                     /locus_tag="ACG8WP_000706"
     CDS             34685..35776
                     /locus_tag="ACG8WP_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9027037.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35797..36339
                     /locus_tag="ACG8WP_000707"
     CDS             35797..36339
                     /locus_tag="ACG8WP_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFP9027038.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36534..36791
                     /locus_tag="ACG8WP_000708"
     CDS             36534..36791
                     /locus_tag="ACG8WP_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027039.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36805..37137
                     /locus_tag="ACG8WP_000709"
     CDS             36805..37137
                     /locus_tag="ACG8WP_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027040.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37182..37730
                     /locus_tag="ACG8WP_000710"
     CDS             37182..37730
                     /locus_tag="ACG8WP_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFP9027041.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37737..38189
                     /locus_tag="ACG8WP_000711"
     CDS             37737..38189
                     /locus_tag="ACG8WP_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027042.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            38286..38627
                     /locus_tag="ACG8WP_000712"
     CDS             38286..38627
                     /locus_tag="ACG8WP_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027043.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            38995..39186
                     /locus_tag="ACG8WP_000713"
                     /pseudo
     CDS             38995..39186
                     /locus_tag="ACG8WP_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT        13352 a         6114 c         8295 g        11473 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aacccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 ttaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga ttgagattct catcggtaaa ctaattgcat acagagatat gaataagtta
    13381 cttaacaaag agttagctga taatatagga attagtgaag tattactcag ttatgtgtta
    13441 aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt
    13501 aatgaaaaag aaattttgaa ggagattaat cgtgatagtg agatatttag tttatcaaaa
    13561 gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg
    13621 cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttat aagtagatgg
    13681 gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta tcttggtaag
    13741 gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc aaaagatgaa
    13801 agagttagcg attttaaaaa gataaattct tttagtatgg gattaaggat gaggaaaatt
    13861 aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc ttcaatttca
    13921 aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa tcgaattatc
    13981 caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat ataaaaatag
    14041 tttacgtcga tacttattaa gttatcgacg ttttttatta tagaaaaaat ttataaaagg
    14101 aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt actggcagct
    14161 tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa gtaatacaag
    14221 aactaactgt tatgaataac agctagataa aatgggctat gtatgttatc caacaattta
    14281 gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt attgatttgt
    14341 agtgtttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc tgcagcgtca
    14401 ggatgggaat ccataccatg gagattcgga gatctgtgag ggaatactta cgtggttaat
    14461 atgtcagttg tgttggacgt agtaatggtc atagtgggta gattgactga attcttatat
    14521 gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga tcttttctgg
    14581 aaaagtaatc ggataatgcg agattcgtac ggtctttttt aaatttctct aaaaaaaacg
    14641 gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa
    14701 tgattctatt tatcttttta catctatcgt tttttcaagt agccgcaaat tatatcagca
    14761 ttcaacaatt tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt
    14821 gaagataaaa aaagaatcag gttttactga gtttttcttc aaaatttgtg ttattaggtt
    14881 aggacaaaga taagaagaag tatgaagatt taaaaaagga atgaggaaaa gatagtaatt
    14941 tccgattatt ggagtttttt tatttataaa gaagaagcga ataagttgtg attcctagat
    15001 ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt tttagtgttt aatacacgag
    15061 gacaaagttt cttaggtatt gaaggcacac gtgtaacggt tgatgggtgg tgtgattgtt
    15121 tcgaggttgg aaaacatgtt gaacttatgg catttactga gctaaaagat aagaacaacg
    15181 tggaattttt cgagagtgat attggttggg acgatcatga agaagtttat ggaaaattta
    15241 ttttgcggaa gggaaattct tatacgaatg gggaagcata agtagcgatt tgttagagat
    15301 cattgatagg attgaaattg tcgggaatat ttatgagaat ctagagttat ttgaggtgat
    15361 agaatgagtg atggttttga acaagtatca ataattaaaa atcaagttcg ggagattctt
    15421 agaaaaaaag gctatttggt agatagttat tttgaaggag attacgaaac atgggtaggt
    15481 gtctatgctc gtccagagaa taaacctacc tatttagatc ctacgacgtc agaagatgcc
    15541 tatttacaaa ataggtatcg agtagatggt tttaagcaag actttgcgga gtggtttgaa
    15601 tgggaaattg aaaacggaga agtaaaagaa gaataagagt acttgttgta tcaaaataag
    15661 ccagtagaac aaccaaacca gtagctagta atgacaataa tactgaggaa tcggatcatt
    15721 atggtatgat tgatatttct cttccagaag atcttaagcc agatcctgta acaattggca
    15781 atagtgtcat gatttttgat tcattagatg aagctatgtc atatggttgg aaagaaatta
    15841 aggatgaagg tagtaattgg tatggcatga gatttggtga tggtgaagtt gccaaaaaag
    15901 ctggtattcc aattgggaaa tggacagttg atttccacga agcaggttct ttttttgttc
    15961 tgtaattata gtgggagggt aaaatcattg atgaaatgtg ttcagataag agcgccagat
    16021 aaaaatagaa gagacagttc acgtcgttat cacttttggt aacggcgttt tgttttatta
    16081 tataaaattc tcaggaaaag gaggaattct tgtgtatgca aaaacgaaaa tgaattggtt
    16141 gaaagcactc gcattggtgg ctttttctca attctgtttc ttggtgggaa ctgctcaagc
    16201 acatgcagaa gaaacgacgg ctatacaacc tacgatggaa gccattgccc aagtcgcttc
    16261 tgaagtgaca gaagtccaac cagcagaagc cggggtccca gctggtgaag ccactgttga
    16321 acaggcccct gtgacagaag cgccaacggt caattccaaa acgactctcg atcagacgat
    16381 tggggaaaca caacacccag ggaatagtcc tggtacttca caaccagttg aagaaactgc
    16441 ctcaattaca acccctgctt cagaaacgtc cactctagaa ggagaaaaag ctgccgaagc
    16501 aacgccttct gtgcagccag agtcttctga gaaacaagcg gaagaaaaga aacgaataga
    16561 agaaacagtg gaacaaccag cggctactgc tgacaaagaa ggaaaaaatg aggaaaacgt
    16621 tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa ccaacgaaac caaacaaaga
    16681 atctacggct agtattgaaa atcaaaagaa aaaagagcag aacattccaa aaacaggtac
    16741 acctacactg ttagtaacag taccaaccaa tcgttcggtt cgtgttcgcc gtgcaaccaa
    16801 tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa aatattggtg ttacttctag
    16861 tccgaacaat ctaaacttca ctttaaatta tatggttcgt tttgagatta atggacaagt
    16921 ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt caagtttata cagctactgc
    16981 tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa acaagcttga tctcttattt
    17041 aggatatgtc acaaataaag acaaatccga tgaacaatac attgcggcac aatttatgat
    17101 ttgggaagct ttgggcacaa aaatcccaat caaaaatatc cgtgttgatt atgatcgtcg
    17161 aaaagcagaa attcaacgtt tgattaataa ctttggtgaa aaagcaactt ttaataatca
    17221 aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag aataaaaatg attttttgaa
    17281 atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta tctattcacg gcaatgaagt
    17341 atggattaca gtcacaaaag aggcaccgga taaagcaaaa attcaattga ataaaattaa
    17401 agttcaaggt gcgccattag cctatcgtcg tccaggagct caaacaattg gtgtgttaca
    17461 tccaacagaa cctaatgcgt ctgttattaa tttacaaatt ctaaaacaag gaaacattcg
    17521 tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa ggtgcagctt ctttagaaaa
    17581 tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt aaagaaatta ccttgaaaaa
    17641 aattaacggc aaagtacagg cagaaattaa agatcttgat cctggcaaat atgttttaaa
    17701 agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa gcaattgaaa ttgttgttga
    17761 acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg atcaaaggaa ccattgattt
    17821 agtgaaagtt gctaataaag atttagtcga ttcaacgaat ccagataaca aaccaaaatt
    17881 agctggcatt gaaatcactt tgacgtcaaa aacaactggc aaagtggtga aaaccgttgt
    17941 gacagacaat gatggttatg ctacttttgg taaaaatacc gttgtctttg atacgtacat
    18001 tctttctgaa accaaaggaa aagaagggta taaactattt gaaccacttg aagtcacaat
    18061 ttctgaaaat gggcaaactt tccattatgt tttagaagat aaagtaattg aacaacgtct
    18121 taaagtggtg aaagtggatc aagaaacagg aaaagtgatt ccacttgcta atacagaatt
    18181 caagattttt gatactttag ccaatcgtta tgtcacaatg gagattccaa atgatacaga
    18241 aacaacggat atctttaaaa ccaatgaaaa aggctttttc ctaactaacg gtacacttac
    18301 ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc cccgtaaatt acgttttgaa
    18361 tcaaaaccca ttagtgttct cgattaccaa tgatacagaa ccgatcaaag tgatccattt
    18421 tgcgaaccgt caagcacgga aaagtgtaaa attattcaag tatgaagaat ggaacaagaa
    18481 gaaaacgccg ctcgcaggtg tgaactttac cttgtataac ggtaaaaatc aagcaattgg
    18541 tgaatatacc actgatcaaa acggggaaat tttagtgaaa ggtctagttg ccgatgacta
    18601 ttacttcttg gaaaatgccc cattagaaag tttccaacca aatacagata aacaagcatt
    18661 cacggttgca ttcaaacaaa atgcgccaga tgatggggaa acgttagtgg tttctgtacg
    18721 taattacttg attccaccaa aactagaaac acaagcgtca aataaagccg atggtgaaaa
    18781 agtggttgat ccattagaaa cgatcgtcat taaagacagt gtttcttata ccaacttgtt
    18841 tgtcggaaaa gaatacacca tttcaggagt attgatgaac aaggcaacga atgcgccaat
    18901 tttacaagat ggcaaagaaa tcacaagtac cttggccttt attgcagaca aagtcaatgg
    18961 cacaaaagac cttgaatttg tggttaatgc tagtgttctg aaaggtcaaa cgatcgttgt
    19021 ttttgaaaaa cttttccgtg atggcattga agttgcagca catgcggata tcaacgatga
    19081 agcccaaagt gtgaaagtga ataatccaac cgttcatacc acggctgtct ttgaagacgg
    19141 attgacagtc gcagacccaa caggattgtt taccttaaac gataccgttg aaattaccga
    19201 gattcttcca ggaaaagcat acttggtcaa agggatctta atggataaag caaccggtga
    19261 acccttgctt gtgcatggaa atacggtaga aacgagtgtt ggctttattg ccaaagcaga
    19321 aaatgaaacg gtgatcgtac catttacctt tgatttgaca ggtataaatg gtcgtgaaat
    19381 cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta gcaaaccata aagacatcaa
    19441 cgacaaaggg caaacggtac gagtaactaa cccaagtgta cgtacaacgg ctacacataa
    19501 acccactggc ttgaaagagg ttaatccatt aacgaaagtc accattacag acgctgtgaa
    19561 ataccacgat ttggtcgttg gaaaaacata cacagtcaac ggtgttctaa tggacaaagt
    19621 aacggaaaaa ccagtgctag tagatggaaa agaagtcaca agtaccttaa cgtttgtggc
    19681 agaaacaacg gatggtacag tcaatctaga ctttattcta gacgcacgtg cgttgcgagg
    19741 caaagaagtc gtggtctttg aagatctgtt tagagaaggc gtattattgg cttctcacgc
    19801 agatattacc gacaatgacc aaaccatcaa aatcagtaat ccaaccatta cgacgaaagc
    19861 gacgaataaa aatggcggaa aagagatctt ggccttgcca aatcaaactg tgcttgaatg
    19921 ggtcaaagta gacggtcttg cgattggtca aatctatcgc ttaattgttc aaggctactt
    19981 aacaccagac ggcacaccat ttgaaggcac tcatgcggaa aaagtctttg cggccgataa
    20041 agaaatgatg gaattcttat ttgaattctt agtggacggt cgcaatcttg caggaaaagg
    20101 cttgagtttt gcggaatggc tccaagcgaa aacggaagat gagaaagaaa cgtttgaaga
    20161 agtggcgaaa cacaatgtag atctaacaaa caaagatcaa acggttcaaa ttgtggaagc
    20221 gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt aaaccagcta ctgttgtacc
    20281 aatgttgcca aaaacaggtg aacaaacaag tagtatactt atggttcttg gattagtagt
    20341 attagtcatt gctttaggta taacacttta taaagtacgt aaagccaaag attagtcatg
    20401 aggaacaagc ggttaggatt tttaaccgct tgtttgattg taatgtagaa atatttagaa
    20461 tttattttgg tttttaagag gaggaaaaac tttatggcag aaaaagcatt gcgtatgaag
    20521 aaagagtttt taggtcaagt atttttattg ggttcacggg aacctaatcc accaaaggaa
    20581 attaatggtg tgtctgggcg atatgttcaa ttacaagcag agtgttcgcg agaagaacag
    20641 cctgcttttt ttgtttatct cacagctgaa gaggataaaa gattacgatt accagaaaaa
    20701 gaggtgcgta tttcatttga agaagcttat tttgtttttc gtaaagaacg agtgaatagt
    20761 cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa ctggctatca agatgttggt
    20821 gtctttttaa ctggtgtaaa aattggggag gttttgtaag atgggattaa aatttccaaa
    20881 taattatgac attacagaag cattagatga aacttgttta ggagccgtac gctttggcgc
    20941 attagaagag tctgatcgtg tatttgataa tgaaacaggc gattatacgg gagaaattcg
    21001 atcacagcga gcacaattag tttttggaaa tccagatgaa ccaaagattg cacgacgagt
    21061 taaatttcca gcagatgtag atttgactgg ttttaaacga cgtgatcctg tgaaattaat
    21121 tgatccagaa tatcatgtgg tatatattcc agcacaagat ggcaatcgtg aatattcaga
    21181 tttagtgatt tatgcgaaag gattagagaa agtagaagct ccaacaaata atcctaatat
    21241 gaaacaatca caagaaaaag gtttagatca aaaacaacca agtgttgatt ctaaggaaaa
    21301 agagaaaaaa taaggttttt ttgaaagaat aggaaggagt cttcctattc tttttatcta
    21361 agatagaagg aaggggagat tttgaattca gataaaacca atcaaatact ttgagagatt
    21421 gtgcttgtta tcaaaatatc caacaattga ttgagtattt tggagacaat ccaacgattt
    21481 ttgtacaaac aaatgcatat atttgtgcgg ttattgggca gtgtatgttt ttacgtgatc
    21541 ttgttcgttt ttcattaccg taccagtgga aacaagatca tcaggaagtg atttggattc
    21601 gtatagtacg aatatgtaat atgtgtgcac acccttataa aaatcgatta aatctttttc
    21661 gattatgttt ttttactaaa tgtgatattc ctgtattagt tgctttttgt caaaagtttt
    21721 tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca attgaaagct cattttttca
    21781 agtatcgtgg tcggcgaatt cgctatttca atcgttattt atggttttgc tattatggtt
    21841 tctttttgtt tccatttttg attctgttag gttggtttag ttggaaagta ttttatccac
    21901 gtttaagtac gttttcgaat tggcaaatgt atcttattcc tggaataagt attgtgttgt
    21961 atttgttgct ggttagtggg ttaattttag ggttgatgca ttggtcgcga ctaaaagaag
    22021 gatattttgc ctctgtttat tggcgtcagt tacttgttcg aatgttaatt gacaatcggt
    22081 ttgtttatac aaagaaacaa gttagtgaga aacaaacaag ggaaaaaatg agattaccaa
    22141 aagtttattt ttatcaaaag aaacaagaat tagtggtttc ttttccttta gatggtgggc
    22201 gttttcacga acgattttta aaaatgggtc atttgttatc tgaagtattt ttgcgtgatt
    22261 taattcgtga agaacgagaa aaagcccgaa tcaattatgt attcctttca gattctggtc
    22321 ggattcctat tgatcaatgt atagcagaaa atggacgtat tcatttaatg aaaactttgg
    22381 attgggtata tgatcaatca ccaaatatgt tgattgctgg tgcaatcggt ggtgggaaaa
    22441 cctatttatt gtattcttta attcaagctt gtttaagtgt tgggacagta gatatttgcg
    22501 atggaaaagc ggcagattta gcagcattag gtgacgtcga tgtattcaaa ggacatgtgt
    22561 tttacaaaat aaatgaagaa atgattattt gtttacgaaa tgcattaaaa gaaatgaatc
    22621 aacggtatgc ctatatgaaa accatgaaag atcctaaata tgaaccaggg aaaaattatg
    22681 cttattatgg gttaccaccg cattttatct tttttgatga atgggcagct ttttatggtt
    22741 cgttggatat ggaagcgaga aatagagtcg ataaattgat tcagccatta gtattaaagg
    22801 gtcgtcaagc agggatgtac attattttag caatgcaacg accagatgca gaatattttc
    22861 caagtggtgt tcgggataat ttaacattcc gtatttctgt tggtcgattg tctcccattg
    22921 gttatttaat gacttttgga gaagatttta aaaataagcc attttacaac aaagatcaac
    22981 ctggtcgtgg atatgctttt ccaggatttg atgtacctgt agagtttttt gcgccatttg
    23041 tttacaaaga ttatcacttt atcaaagaat ttgagaaatt tactgaaatg attgtctatg
    23101 attttacagt aaaagaggat agtcaagatc aaacgatggg agatgataat cagttgttac
    23161 aagaacaact agagcgccaa atgtatttag caaaagatcc aaaaggaaca agtttttctg
    23221 atgcgcatat tttgtaatca actaaggagg tgaagttaaa tggcagagag ttgttattat
    23281 agagtagcta cagcaatacg aatgattaat ccttctcttt cttccagaac gttttatgat
    23341 tggttgaatc gtattgaaca agtaactgat taccgctttt tgcgcaaaga acgtgttttt
    23401 acaggaaaag ttattaatca agtattgcta acgaaaaaag atatagaacg attgactcga
    23461 ttgtatcatt atcgagtaga tttagaagag gatttaactt tgtcaattta tcgcgttttt
    23521 tctcctgaaa aatatagtga aataacaaaa ttagatcatt taattttgta gttattatct
    23581 atcgggaggg ctctagacgt agtctggtag ccgcactctg cctggcggtg cgcattaagg
    23641 ctgcgaagca tgggcctata atgcgcactc gctagcagcg tgtgcagctc acaaagaggt
    23701 cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga tatataatat ggggggcaga
    23761 aataacataa cacatagaaa aaagttttat aaaccctttt gtatcaagtg attatctaag
    23821 gtcagcagtt gtactacttt tcccaaaaaa cgagggggac tagttgatat atcggtgttt
    23881 ctaaagggtt aaaaaaaatc gattttgtta ttttataaaa ggagcgagaa aatgacaccg
    23941 attgagctaa aaagctacag aaagaagtta agtgcaattc ataatataag tggagataaa
    24001 aagccgctat cacaagcaag atttgcgcaa ttgatgggct gctcaaaaga ttacattaga
    24061 aagatggaac aagggaaaag aaaaatccca gaggattttg aacaacgaat caaggaacgc
    24121 tttcgattgt atatgaaaag cggaatagat cttcgagcaa tgattgatta tttgcgtctt
    24181 tccttttttg ggacagacta caaaacagtg attcaacgtg tattacgaat ggatctgaca
    24241 gaatttgaaa cacgtccaac gggcttgtat ctttttgact gtctttctgt tcgtgggaat
    24301 atctgggtgt tctggcattc aaaacgaact gtaacagaaa atgtacttgt gcaattttca
    24361 ggacaaggtt gccgggaata tgaggaagtc atgcgtgaac aacaaacaga ttggcgaaca
    24421 tggctatatt ccatttggaa aaatcaagga aagttggaag ctggttatac acgtgtgcaa
    24481 tgtagccgat tcgatattgc gttagatgaa ctttggggat tggaaacgga agaacatttc
    24541 gatctctttg aattactaga aaaacaaaaa gcaggtttat tagaaatgga ctttaagaga
    24601 ttccagaata ttggcggttg ttttttagat gatggctatt ttcgaagtga aggattatct
    24661 ttgtattttg gatcgcgaaa gagtcctctc ttttttaatt tttatgagaa acgttttgaa
    24721 attgcgaacc gagaaaaaat ttcagaatta gaagcattaa cgaaatatgg tatttataat
    24781 cgatatgagt tacggttagc caatgagaaa gcaacacaag cagtagaagc atttatttta
    24841 ggtgattcac cgaaacgttt aggagagatt ggcgttggtt taattaatgc aaaacttatt
    24901 gtttgggata gactggaaag tgatgcacgg attgttgata aacgatgggc aaaattattt
    24961 cgttcgatgg aagaattgga ttttacagta aagccagaaa aattctcatt agatcgttca
    25021 gaacgctggt ttttgaaaca agtagctccg acattaaagt tgttacaaga agcagatcgt
    25081 attttaggaa gagactttgt acaagaaggg attgataatg ccgaactaaa tgacgagcaa
    25141 caaaactatc tagaatggat gggaacgtca ggagggaaaa ttgcatgaat gcatatacga
    25201 caacagaatg gctgcgcctg ttggatttaa aggctgcaat cgaagcatta aatgaaaaga
    25261 tggttgattt gtcttatttt cgttttcgag ttccttatat tgaacaagcg gtaaaagctg
    25321 gacgttatca ggaaaaagag aactggcaag aaattgcacg cttattagaa gttcgtaagg
    25381 gatacgaaca agaactcgaa gagttggaat ttagccgaag gaaaggaagg ctagaattca
    25441 ttcgctttta tcgattttca ttgccaatac ctgcgatttt agcggttaaa aaaggctgcg
    25501 ataaaatgaa gatatatgaa aactgtgtgg cagcacttag cagcgaaaaa cctcttatgg
    25561 aagaaatttc attggtgact gtcacttggg aaatgagtcg ccagccaaca gaagaacaga
    25621 catatctttc tttagaagag attgagatag aattagagga aattggtcgc tatgccactt
    25681 gttctaccta ttgtggatcg gtcattagta ttgctggtgt gattgtctaa atgaaaaaaa
    25741 ttatgattta tgttggtgcg tattggtcac gtgatccaac agtattagaa aatccagagg
    25801 cggtacacta ttgcttgcgc caattattct atctgtataa agaacgatta gaatcgttga
    25861 ttcgtcagtt accatatact gacagacgtt tagatgaact tcttctaagg tatccagcaa
    25921 tgtataaacg acgaaaaaat agattgttac cagaagagta tcccattgaa aaacatgaac
    25981 ttgaagggcg atttgtggct tatttctatg atgatgttcg tatgcgctta gtagaacaac
    26041 gaatggaaat agaaaatgat cgctactatt ttatcaatta ttgtaagaag cgtaagtatc
    26101 aagtaactga tgatttttat gattgtattt tacaagatgg ggaagtgatt ctatccaaaa
    26161 ttgctccaag ttttcgtgaa cttgtgccga ttgatttttt gaaaaaatgt catttgcgga
    26221 ttcttccgta aaaaagagct ataaaaggaa agaaaagtag aagccaaatc ggtttctgct
    26281 tttttatttt ttttaggaag gagaagatgg tatgtcactg gaatttttag gagagccaat
    26341 tgaaaaggaa gagttcgttt cgcattatat ggatttgttt attcaggtga taaaaaactt
    26401 tgtgcgattg atgaatttta tccacgcgaa caagactatc tagccgctga acgaataaaa
    26461 atcagtttat tatgggaaaa attcacagat gaaaataacc gaccgccaga ttatcgctat
    26521 ttatcagatt gtttaacgaa taatgtgctt tctaaagaat acctatttca caaacgtgag
    26581 aaaaatcaaa tgtcggttga acatttttgt gattgttata ttgcattatt gcgtagtcaa
    26641 cagttattaa cagtagaacg atatggtgca gagtttggtt atgtcatgca agctgagcgt
    26701 catagagcta caagatgcta tgaaaaagaa gatcaagtga ttgatggcta tgaagaatta
    26761 tgtattcgaa acaatcgcta tttgcaacaa cgtttatatg aacaattgat gatggatttt
    26821 tcaaatctct atagagggta tgaatgttcg atgattcaag aaaggtagaa gaaaaggggg
    26881 aatttttatg tggcgattat tatttttggt ggtatgtggt atggcattgt tctatgtgta
    26941 tcgtgtctat ctctatccac gaatggaacg atggacaagt tggtatagag atcctttaaa
    27001 acaacgaaaa ggattgaagc ggtatcgtta tgaattactt gttgaatttt cggatgggcg
    27061 acaagcacag tattattttt ttgcaaacta tccaaaatac aacaccagtg aactattgat
    27121 tcaattattc aaaaataatc catatgttgg gttagaagaa aacggacggg agttctttta
    27181 tcgttcagat aatattagtg cggcgtctat gaacaaaaca cgaattgcat aaaaaattgg
    27241 aggaataatc atgaaaaaaa caacattagc agtcgtaaca acactaggaa tttcattatt
    27301 ttcgtttcaa ttagggagtt cattggttta tgcagatgaa actgtagaaa cacctaagtc
    27361 agaaattgta gaaacattgc caggggaagg attaccagat gttgagacag agctaaatgg
    27421 aggaacaacg gaaaaaccag cagaacctga acaattgcag tcagagaaac ctgaaaaaga
    27481 gccagtgaca ccagaaccag agtttcctga atcaacttta ccgactgatt cgatcgaaga
    27541 agaaggtgtg catagttcaa caacaacgga ttcaagtata gtcacaactc cggaacaatc
    27601 aggaacaacc agttcttctg aaaagaatac tgattcgtca agtacggaac agccaacgat
    27661 gtcgtctacc agtgaagaac aaaaagaaga accgaaacaa cctgttgaaa aatcagaagc
    27721 aattccaaaa gaaaataaac ctattgttcc agcaaaagaa gtcacagtat cggtaacccc
    27781 aagcggtgaa attacgacga atacttctca aggaatgagt gtaccgattg tcactagtaa
    27841 tgtagaggaa ttgacccata ttccaacacc aaccacccca ttaaaagtag caggtgatca
    27901 aacaattgtt ggtgtaaaag atggtattcc attaatccaa gatagtgaag ggaatttggt
    27961 aaaggatttg tcgattcctg taaagaaact tccaagtggc aatattgaaa tcaaaacagc
    28021 agatggaaaa acaaaagtct taccaaagac tggtgaagaa atacatgttg cactttctgt
    28081 tctaggagga gtgttgacaa gtattgctgg atttgttgga tataaaagaa agagaaaagg
    28141 aatagaaaat gagggtaatt gaatggacta tttttcagaa aaacaagctt atcgttgtca
    28201 atcgtacgta ctagtaaaaa catactagaa ggagtagctt atgaaaaaac gacaactacg
    28261 gcggaaagaa aaagtttata cagagaaagt accgaagcaa aagaaaatcc attcaaaaaa
    28321 agcagtgaaa tggatttggt gtttggtttt atttctttgt ttttcagggg gcttggcatt
    28381 ttttcaatcc atgaaaacga aggttcaagt gtcgtttttg aaagatgaaa tccaacaatt
    28441 acaaaagaaa gaagtacaaa gtcggtcaga attaacgtac acacctgaaa ttgaatcctt
    28501 tatgaatcgt tttgttgctt tgtatatgaa tacattcgat aatcatgaac aagaacaaca
    28561 acgtcaagaa acattaaaaa aagagtttta tgcacaaaac tttattcaag aaccaggaag
    28621 tgtttcaggt gttcgaaaat taactgctgc ttcgtttatt ggattaaaag aaattgaaac
    28681 cattccaact gctagttata aagtaactta tgatattcgc tcgatgaatc cagagagtaa
    28741 gaaagaaaca agtgtgacaa tcacacaagt actcaatctt cccttttcta tttcaaacaa
    28801 gggctgtcgt attattgctt atccttattt tacggcacta ccaaagaata cagaaaaaca
    28861 acgaatgatt acttatgatg acacattata cgaaacggtt gatgcgtcgg aagcgaaaga
    28921 tgttgaagga tttgtggaag atttcttaga taagtatgcg aaaaacaatg caaaagatat
    28981 gtcatacatg atgaaagaac ctgaaggact caatcaaaca gctaaagttg atcaagtaga
    29041 gatgaaagta ttgcgttata aagaaacgaa aaaacctttt attgtaaaag caacggtcgt
    29101 ctttcgagat ggtgatacgt cacttttaca aaaagaacaa atgacgattg tcattacgaa
    29161 aaaaacaaat cagtattatg ttgaaaaatt aacacataat tggttggata ggaaaggaga
    29221 agaacggaaa tgacactgaa gggaattgta gattttatat taacgcaagg tggctatttt
    29281 ttaataatgg tggttgttta tctgggattg cgaaatttta tgaaatcaaa agtaggaaat
    29341 gcattaatat ctatcgcagc aggtgccgca gtttatttct tcttaaatga tccaacacgt
    29401 gtatttgatg cgtttggtaa aatcattgaa aaattattta tggattagga ggatagtgaa
    29461 gtggattggg aaccttatga ttattcgatt gtttatgcgg aaccttatcg cgtacaacag
    29521 attacaagca attggcgtct tccatttcca gttgcattag tcaatgcagc aacttttttg
    29581 ttggtcgtaa tctttgaata tttattttta cgttcattta ttggttgggt catttctttt
    29641 tttccaccag caggagttgt tttttatata ggactcccct atttatgtgt gttagcactg
    29701 gcaaaactaa aacctgatgg aaaaaaagtg catttttatt tatgggattg gttttgttat
    29761 atgattcgta tgatgaatgg tgattgtgtg tattataact gggacaaggt atcaaacaat
    29821 gcattggaag aattactttt tgtgaataag tatgtttatt tagaggatta gaaaaaggga
    29881 caatcaactg gttgttcctt ttttattggg aggtgaaatt cattggtgct tgtaaaagaa
    29941 ccagtaaaag cattcaagaa aaattgtttg ttaaatggag aggattgtat ctgggcctac
    30001 tatcatgttt ttccacgaac aatttcaaaa aacacaccaa aaaagacaca ggaaataaaa
    30061 gaaatgatgg ctcgtttact gttgctttta gcagattatg aagatgtgga gttggtgatg
    30121 tatccacgaa caatgaattt acggacgaag tttgacacat taggaaaggg attttctccc
    30181 gatagtcaag caattggtgc gtattatgca aaagaaacag tgacattgtt ggaacgagaa
    30241 ggtcaagtct atcgtccttc atttatttta ggaatcaaag tcaaagaaaa agcaacggtg
    30301 gagagttttc atgatgtaat aaatctaatc tggttagatt tagcaggaag tttagcacgc
    30361 ttatttggat tagaagtaga tgatttggaa actttgtttt ctgaaagtga tccttatgta
    30421 aaagaagtga cacaattact ttatagcttt tctattcgtc ccttagtaaa aaaagagctg
    30481 ttgtatctca atcgtctgca atatattcga ggaatggctc acaaagtaga agaacaaaat
    30541 aattgggaag tactgaaaga caatcaagga acgtatgtac gtataccgaa ggaacataaa
    30601 ggccttcttc aattagaaga tgagttaggg agtagttatt tagcattgtt gcctattggg
    30661 gaaacacctg aaaatatgag aaattgtgat ctatttcaat ttgctcagga tctttctttc
    30721 ccagcagaat tgcgaatgaa aattcactat ccacgcttga aaggacaggg aggtattggt
    30781 ttaaaactga attggctatc ggaacggttc aaaacagagc ataaggaatt ggttcaaaat
    30841 gatgaagaac ctagtgaacg cttattaact gttcgaggat tagtaaaaca tttaaagcaa
    30901 cgagtagata atgggcaacc tgtcgtcgat tggatcgcat gttttattgt atacggtcaa
    30961 acggttgaag aagttcgtca aaatagtgca acggttattc aacaacttca tacgattatc
    31021 catatccacc gtgcaaaaaa tgatcaacga gaattgttct ataaatgttt attagggcaa
    31081 ccattgggac ggcagttaca ttggaagcac cgaacaactg tagaagcagt tggcgaatgg
    31141 ttgttttcag cagtatcaga attaggtatg gaatccggct ggtatattgg tcggcaagat
    31201 acattagggg atggtggttc tcgaggagaa aaaacaccat tagaagaatt aatttatgcg
    31261 tcaaatcgtt ttgtcttttt gaatcttcta gccattgcgg aaggaatcaa aggtgcgttg
    31321 tttgatgcgc ctcatgtagc agtaactgga aaaaccggta aagggaagac ctttttagtt
    31381 ggcttgctct ttttgtatag ttcgtttatg aatgtacaaa gtttatttgt ggatcccaaa
    31441 ggagaaaagc gcaaatggtt tacgaaactc attcaagacc cgtattatca gaaacattac
    31501 cctttgtttg tagaacattt gcaaaaaatg agttatgtca cgttagatgc tacaaatccc
    31561 cgtaattatg gtgtattaga tccaattgtt tgtttatccc gagtagatgc gaaacaagta
    31621 gcccaggata tgattgatga gttaagtccg ttaggtacca atcacctttt aaaaggggcg
    31681 gtattaaagg gaatccaaga agttttagtt caaaaagaag ccggacaaca agttggcttg
    31741 atgcatgtgg tcgactattt agaacaaatg gaacaaaaag aggttcgaga atatggtgag
    31801 tttttacgct tgacggtaga agattcgatt ttacgtttag gattttctta tggagaaaac
    31861 ccgggattag actttaatgc caaaacaacg attttagaga tccaagattt gaaattacca
    31921 aatgacaatg tccgaccaga actatataca gatgcggatc gaaagtcttt gtgtttgatg
    31981 attagtttag gtcgtttttg tgaaatgttt ggaaaacgag atagtagtaa gaaaacagcg
    32041 atttatttta cagaagcatg ggtatttaat aattcgaacg caggacgtag tattattgca
    32101 gcaatggcac gtgtggggcg ttcccagatg aatcaattag tgttagatac acaatttatt
    32161 ggtgacttag gttcagataa agaaaaaggg aattttggtg tcgtctttgc ttttgatgaa
    32221 gacagtgagc gagaaaagat tttgaatcat ttacgtttgg aagtaaatga agcgaactta
    32281 gctgaaatga gccacatgat taaaggacag tgttggatgt tagatcccta tggacgtgta
    32341 ggaaaactca atgtacacag tttgtttgaa gaaatgaccg ctgcattaca aacaacggaa
    32401 aaaacaagtc actctcaagt agaagaacag gatacgttaa tcatgcaata gaaatgaggt
    32461 gaaacaatgg cttatataaa gcataaggaa aagaaatggt gcgttttgtt ttttttaacg
    32521 attggattcg gactgctttt tgttggtgga ttggtgtatg cggcagaaaa gcaggtggat
    32581 caatcgatct atgagattgg gaaatttcat attgatacct tttcttctta tttatctgat
    32641 agtgaatttt cattaggcaa aatgttattt ggggcaaatg gcacagattt tctaaaaatg
    32701 atgacgaata tgttctttag tttaagtaaa ttgatttggc aaggccttga ttttgtcatt
    32761 gagaagctct atgaaggagc tgcaatgaac gagttaattc accagttttt cactttttcg
    32821 gatactattt attcaaaaat atacgatcct attggtgcgg ggattgtcgt cttgtatgtg
    32881 ttatatctat ttactcttcg tatatttaga gggaaacatt acgcaaaaat ggtgtttatt
    32941 cgatttctct tcgttgtcat tttttcaatg gtttggtttg gctatggtag taacgtacct
    33001 agtaaaggag aagcttttac caaagggtta aatgatttgt ccgtggaggt agaaggcttt
    33061 attttttctg ctacaaatag tctagatgaa ttggcgacaa aagatgtcaa tggtgtttta
    33121 gtgccaacga cacaaaagga agccattcaa aaagtgcgcc aactttatta taaagcggct
    33181 gttgttgatc cgtatatatt gttaaattat gggacaacaa atttagaaga attgtatcaa
    33241 gcagagattg aaccacaaga atttctagcc aaggaagcgt caagtgaaga attatctgat
    33301 attgagacaa aagtgaccac tgctgcagga gaagatagag ggaaaaagaa ggcttatcgt
    33361 gtctatattc aaccaactaa agctgtttat aaagcagtcg ttggttctat ggtgccattg
    33421 cttaatctaa gtattggatt accacttgta gtgattggca tgattcgttt tatctttcaa
    33481 atgttggtgt tgtgtttatt acttgtgatt cctttttcat tactcgcttc ttttgttcca
    33541 gtatttgatt atatgctcat gaatacctta aagtcctttt tgggcttgat gtttcaaaaa
    33601 tctttttaca gtgtgattat tttggtggca tttttgatct ataacatagt agacgattta
    33661 attccaatga atactgtttt tggtttttta ggaaatatgt ttgtcaaagc actcctatca
    33721 attttactca ttgtaaaacg gaaagattta ttaaagaaac tagggttagg agctgcagat
    33781 caaactataa acaacacgaa aaacatagca agtcattctt tgtctcaaat gaaacaactg
    33841 cctggtcaac tatcaactgc tggacaaaat ttggcaatca aaggtgcagg attagcaggg
    33901 aatgtgtatc gagcaacgaa tccactcgct tctgcagcga tacatggatt tcaaaagttt
    33961 caacaaaaac gtacaccaca agaatcggta aaagggaggc aagtggttaa tccctttgaa
    34021 ctaggtgggc gttcaaatca agccttgcct tatagtggtc gtcatgttcg ttatcatggg
    34081 gtagagcctg aaatgtcttc taccaaatcg aaaacagagt ggaattctgg tctttcaaat
    34141 ggtttgattc caaattcgtc catgaattca gtaagaccaa aacaacaggt acaatcattg
    34201 gcacaattga gtaagcaaca agggaataat caaagaaaac cattgttaaa taaaggtatc
    34261 gcttttgttg gtcaatcgat tccgttgcct tctggaaaag tacccgtgcc acaattaaca
    34321 gctgctggtt ctctttcaca acagattcgc caagtgaaaa atcaggcgaa aaatgggagt
    34381 tatccagcaa tgcagcagtt attgaaccaa tactctgttc ctaattcgtc ggtagccact
    34441 ctatcaggaa atcgttctgt gcaacaaccg gggcaacgtt cgtctgaaag taaaccgtcg
    34501 atttccgcac caaaatcgtt acaacctgca ttttcttctt tagataagcc aacagagcaa
    34561 tcggaaaaaa tagaaaagga tcctaaaaca ctgccgattt tggacgcaaa accaggaact
    34621 gattttactt taccaaaagg acgaacagtc caaaaaccat tatcgaagaa aggatgagga
    34681 agaaatgaag aaagggtgtc taggttgtct tcttttcttt attcttccct tttttctatt
    34741 gccattcttt ttttcaggag aactttctga gcatggagga gaagctacag gtctagctgt
    34801 cgcttttaat ggggaatata ccgaaggatt accgattttt tcagagatta aaggacgtgg
    34861 tccaatcaca gatgaacatg cgcaagcagc agttggtgca gctgtaaagt atcagttatt
    34921 accgagtgtg attctttctc aattaggcta tgaatcagct tatggacagt cacaatctgg
    34981 aagaaacgat acgaactttt ttgggattac ctggtttcct ggttgtccct acccacaagg
    35041 ttctgcacgt ggaattggtg gttctgaagg tggtttctat atgaagtttc cgaacttaaa
    35101 agcttgttat gactattatg gctttatggt ggcgagccaa agcaacttta atcaatgtgt
    35161 gggcaataaa gatcctggaa gttgtttatt gattctaggt cgtggaggct atgcggcagc
    35221 aggtattacc gaagggagtc cgtattatcg aggagcaatg agtatcataa cgtcctataa
    35281 cttaacggaa tatgatactt ttgcgatcaa gcattggaat agcctaccaa caaaaaaaac
    35341 ttcgcttatt gcaggaaaaa gtaaaggcaa tgcgaaggtt ttggatcgga tcattggacg
    35401 atccgtaaat aatggggaat gttatggtgg tacggcatgg tatgtatctc aattaggtgg
    35461 cccacagtta atgggttctg gacacatgtt tgcgatgtta ataggggaag attataactg
    35521 gacaacctat ggttggcaag taatcatgaa cccaaaacca agtgatttac aagcaggaga
    35581 tgtggtgaat tggcaagcag gtggtgcatt gtctcctgga atctatggtc acaccggaat
    35641 tataacagat gtttcatatg gcggacaaca tttctctagt gtggaacaga atgccgagca
    35701 aggacggatt atggctcggt atcaacgcac gtacaatcaa acaaaaattc gtagtctggt
    35761 tcgtaaggtg aaataagaaa ggcagtgact agaaacgtga aaaaagggat aattttagta
    35821 tttagttggt tatgtttgat aggtcttact ggttgtcatt ggttttcttc tgaaaaacaa
    35881 aaaacacaat tgagaacgac agaagaaaca atcgaagcag taaaagaaac aagtggaact
    35941 cttcaaagta ctgaaagtaa cagtcaaatt ggttcattag aagacgagac agcgcaacaa
    36001 atgcaaatag atgaaatgac aaaaaatttc ttaaaagatt ttgctagaaa atggagtaat
    36061 ttcacagatg tttataaacg caatcaagct gtgagggact atatgacaga tcgttgtatt
    36121 aaagacaata gtattgatgt agacccgcat gtacaaatga aaacaactgg gaaactttac
    36181 caggtgacac aagatataga gaatccggat caatatttgt tatttggaga agaaacaatg
    36241 aataaggtta ctcaatttgt attacttcaa ttgaaagtag atcaagaagc tcaaaagatt
    36301 gatgcgatca agatttatta tgtacggagt gcgtattaaa ataaaaaatg aacactcata
    36361 tttattatac accgaagaaa tagaaaaaaa cagactgttt ttttaagaaa aactatgtca
    36421 ttctttactt gaaaaaagtt gttataggac agaaaggata aaacaaaaac ggaaaaaata
    36481 tgtcttatga ataactataa gaactattct attttttagg agatggatac gagatgaaaa
    36541 aactaattgg ctatagtgtg atgtgtattg gatttgttgg tgtgggattg tttgtatcga
    36601 ctgaatctgc ttctgcggct gaatggtata atcctatctg gtataatccg aacattgaat
    36661 gtcaaaaagg ctatccgtat ggaccgacaa atctgaaaaa tggaaaatgt agagtaaatt
    36721 ggaatggcgt tgtagatagt gtaattactg gttcaatgga ctctgctcta catggcttta
    36781 cgggtcacta gaaggaggaa gaaaatgtct gaagagaaaa aagaaatcgt tgaatcatta
    36841 gtagctttaa aagattctct ttctaagatt gaatatgttg ataggcaaga aattcaaaaa
    36901 ctaattgatg atactattat tgaaatccaa gatgcgcgtt gtgaaggtat aaaaatttct
    36961 gtagctttat ctaaagtaat agaaaaaatg aaccgttcat tagcgtttaa tggattgaag
    37021 ctagatagac aaactagtct gatatgggat catttaaaag acttgtatga caagtctaaa
    37081 agaagtgaac ggactgctgt atcaatctta aaaggccttt ggggaatgaa ttcataaaca
    37141 agatatttaa aaagtaactt attttttatg gaggagaaat tatgaaaaga ataattaaaa
    37201 tatttggtgt tttaggtgtt ttcttattag gtttaagtac ttttgttagt actggaacaa
    37261 gtgtgtatgc cacagaaaaa gtgaatacag ttgatttaga aataaagaat aaattagtag
    37321 atgaattaac tttttatttt gatacagttg gttttttaga cgaacatggt gtataccata
    37381 taaaggattt tggtgctatt cgtgaacgaa tcagtcaaga taacgaacaa ggaagagcag
    37441 caagagtctt ttatgaacat attattgttc ctagaatgga gaaagttgat tggaaacgat
    37501 atgctggatg tgtagccatt aattctcttc ctggaggagg agtggcttgg gctataggta
    37561 cgcagggaga ttttattgaa gcattaaaag cttttaattt tgaaaaagct gcggatttat
    37621 tattaaaaat tgcagaaaaa caattaggaa aagctgcagc taaagagtta gctaaggtaa
    37681 atattgtagc aggaattgca gcagctgctg ctagctgtgc attagagtaa aagtttatga
    37741 aaagtttaga acaagagcat atggctttta aacaagcaat gtttaaagaa aatatctatt
    37801 taaaccataa ctatattcgt gtttctaagg catgttcacc agtattgaat atgcttggag
    37861 gtggaaatgg attatatcat ttactgtttg tcgatgtttg ttggttaatt tttttaccag
    37921 atgaactagt tatagtcaat gaaaaaataa caagtaaaaa tgaagtgttt aattattctt
    37981 taacaagaat taattacaaa gagattacca agttttcact agaaaaagtt cctttttggg
    38041 gagaattttg tttaaaaatt aaatgtaact ggaaaagaat gtatttctat attgatggtg
    38101 atgatgctct tacattcggt aaaactactt tttctagttt taattttcag tttctattaa
    38161 aaaataattt ttatggttta ttgaaataag gttgcgaagg aagatgtaga gttatttgca
    38221 ctgttgtgtt ggtatatctc ttaatgctgt gtctttttgg ggagattaat tagtggagtg
    38281 attatatgag tatagatgat tgtttagaag aacaaataag attattttta aagtttttaa
    38341 aagttaaagt tcaaaataac tacttcacat gttatagtta tgcacaccct gttttaaata
    38401 gtcttccttt taattcttta gtgcaaggat tgattacaaa tattgtttta ttagtctgta
    38461 caccaaatga aatatttgta aaaaagatag gcagtggtat ttcttcagta agattaaaag
    38521 agaaagaata tactcaaaag attattcata ttcctaagcc tgaaattaag cgttttgaga
    38581 taaaagacta ttctgcttta gggctgttct caattggtta ctggtgataa aaaataagaa
    38641 tactaaaact atacgtagaa tataagtaat cggcatcaat cctaattatg tgattgaatg
    38701 ccgatttttg ttacgcgcta tttttaatta tctgtaagaa gaataatttt attttactca
    38761 tattcgtttt cactttgaag aagtaaattg caatttctta taagttaagc agctatgcaa
    38821 aaggctattt attctattgt gaaatataag atgtagttga aacaggatat atagaatcaa
    38881 aatacgaatc ttattttgat ttttctagtt ttttgaaaaa agcacccttt gatcgttgta
    38941 ttagtggaaa agagaaaaag gagcatactt atcatgtcac aaaactacaa ctaaatatct
    39001 aaacaacacc aatttgatgc ctattgaaaa aaagtattgc gaaatgaagc caagtctatt
    39061 agaaaatgaa ataatagaat aaaagaaaat gggaaaccgc tgaatgatgt aaatgaaggt
    39121 aagtatccac gaactaattt tgatgaacaa gatatctatt ttttatttgt attggagata
    39181 ctagtttaat atcaaaagtt atcagcagtc ataattagtt atccaatgta aaaa
//
LOCUS       DAZKAY010000015        36569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000015 DAZKAY010000000
VERSION     DAZKAY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.015"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8WP_000714"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8WP_000714"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9027044.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8WP_000715"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8WP_000715"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9027045.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8WP_000716"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8WP_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9027046.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8WP_000717"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8WP_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9027047.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8WP_000718"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8WP_000718"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9027048.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8WP_000719"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8WP_000719"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9027049.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8WP_000720"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8WP_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9027050.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8WP_000721"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8WP_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9027051.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8WP_000722"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8WP_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9027052.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8WP_000723"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8WP_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9027053.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8WP_000724"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8WP_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9027054.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8WP_000725"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8WP_000725"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9027055.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8WP_000726"
     CDS             15402..16376
                     /locus_tag="ACG8WP_000726"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9027056.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8WP_000727"
     CDS             16546..17946
                     /locus_tag="ACG8WP_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9027057.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8WP_000728"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8WP_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9027058.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8WP_000729"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8WP_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9027059.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8WP_000730"
     CDS             19454..21253
                     /locus_tag="ACG8WP_000730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9027060.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8WP_000731"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8WP_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9027061.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8WP_000732"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8WP_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9027062.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8WP_000733"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8WP_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9027063.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8WP_000734"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8WP_000734"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9027064.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8WP_000735"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8WP_000735"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9027065.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8WP_000736"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8WP_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9027066.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8WP_000737"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8WP_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9027067.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8WP_000738"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8WP_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9027068.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8WP_000739"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8WP_000739"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9027069.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8WP_000740"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8WP_000740"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9027070.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8WP_000741"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8WP_000741"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9027071.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8WP_000742"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8WP_000742"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9027072.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8WP_000743"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8WP_000743"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9027073.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8WP_000744"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8WP_000744"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9027074.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6705 g        11989 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZKAY010000016        36524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000016 DAZKAY010000000
VERSION     DAZKAY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.016"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG8WP_000745"
     CDS             complement(387..1853)
                     /locus_tag="ACG8WP_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9027075.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACG8WP_000746"
     CDS             complement(2144..4228)
                     /locus_tag="ACG8WP_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9027076.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4753..5112)
                     /locus_tag="ACG8WP_000747"
     CDS             complement(4753..5112)
                     /locus_tag="ACG8WP_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9027077.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="ACG8WP_000748"
     CDS             complement(5142..5483)
                     /locus_tag="ACG8WP_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027078.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="ACG8WP_000749"
     CDS             complement(5480..6154)
                     /locus_tag="ACG8WP_000749"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9027079.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACG8WP_000750"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACG8WP_000750"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9027080.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="ACG8WP_000751"
     CDS             complement(8668..9858)
                     /locus_tag="ACG8WP_000751"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9027081.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="ACG8WP_000752"
     CDS             complement(9879..10406)
                     /locus_tag="ACG8WP_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9027082.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="ACG8WP_000753"
     CDS             complement(10453..13242)
                     /locus_tag="ACG8WP_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9027083.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="ACG8WP_000754"
     CDS             complement(13389..13589)
                     /locus_tag="ACG8WP_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9027084.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="ACG8WP_000755"
     CDS             complement(14042..14362)
                     /locus_tag="ACG8WP_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027085.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="ACG8WP_000756"
     CDS             complement(14865..15983)
                     /locus_tag="ACG8WP_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027086.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16216..16338
                     /locus_tag="ACG8WP_000757"
     CDS             16216..16338
                     /locus_tag="ACG8WP_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027087.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16454..17761
                     /locus_tag="ACG8WP_000758"
     CDS             16454..17761
                     /locus_tag="ACG8WP_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9027088.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="ACG8WP_000759"
     CDS             17882..19081
                     /locus_tag="ACG8WP_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9027089.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="ACG8WP_000760"
     CDS             complement(19108..20376)
                     /locus_tag="ACG8WP_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9027090.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(20432..20498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(20546..21187)
                     /locus_tag="ACG8WP_000761"
     CDS             complement(20546..21187)
                     /locus_tag="ACG8WP_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFP9027091.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(21596..22099)
                     /locus_tag="ACG8WP_000762"
     CDS             complement(21596..22099)
                     /locus_tag="ACG8WP_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9027092.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(22222..22986)
                     /locus_tag="ACG8WP_000763"
     CDS             complement(22222..22986)
                     /locus_tag="ACG8WP_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9027093.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            23094..23369
                     /locus_tag="ACG8WP_000764"
     CDS             23094..23369
                     /locus_tag="ACG8WP_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9027094.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23929..24879
                     /gene="yidC"
                     /locus_tag="ACG8WP_000765"
     CDS             23929..24879
                     /gene="yidC"
                     /locus_tag="ACG8WP_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9027095.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(24925..25326)
                     /locus_tag="ACG8WP_000766"
     CDS             complement(24925..25326)
                     /locus_tag="ACG8WP_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027096.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(25340..26149)
                     /locus_tag="ACG8WP_000767"
     CDS             complement(25340..26149)
                     /locus_tag="ACG8WP_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9027097.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(26136..27026)
                     /locus_tag="ACG8WP_000768"
     CDS             complement(26136..27026)
                     /locus_tag="ACG8WP_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9027098.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(27039..27518)
                     /locus_tag="ACG8WP_000769"
                     /pseudo
     CDS             complement(27039..27518)
                     /locus_tag="ACG8WP_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(27694..28635)
                     /locus_tag="ACG8WP_000770"
     CDS             complement(27694..28635)
                     /locus_tag="ACG8WP_000770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFP9027099.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(28632..29627)
                     /gene="lacD"
                     /locus_tag="ACG8WP_000771"
     CDS             complement(28632..29627)
                     /gene="lacD"
                     /locus_tag="ACG8WP_000771"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9027100.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(29620..30816)
                     /locus_tag="ACG8WP_000772"
     CDS             complement(29620..30816)
                     /locus_tag="ACG8WP_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9027101.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(30834..31562)
                     /locus_tag="ACG8WP_000773"
     CDS             complement(30834..31562)
                     /locus_tag="ACG8WP_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9027102.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(32003..32977)
                     /gene="lacD"
                     /locus_tag="ACG8WP_000774"
     CDS             complement(32003..32977)
                     /gene="lacD"
                     /locus_tag="ACG8WP_000774"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9027103.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(32974..33432)
                     /locus_tag="ACG8WP_000775"
     CDS             complement(32974..33432)
                     /locus_tag="ACG8WP_000775"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9027104.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(33429..34892)
                     /locus_tag="ACG8WP_000776"
     CDS             complement(33429..34892)
                     /locus_tag="ACG8WP_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9027105.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(34874..35806)
                     /gene="pfkB"
                     /locus_tag="ACG8WP_000777"
     CDS             complement(34874..35806)
                     /gene="pfkB"
                     /locus_tag="ACG8WP_000777"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9027106.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35948..>36524
                     /locus_tag="ACG8WP_000778"
     CDS             35948..>36524
                     /locus_tag="ACG8WP_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9027107.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10692 a         7848 c         6210 g        11774 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttta ttctcttgac
    19141 gattggcaag aatagtattc cttttttgac ggctacttgc tgtgcagaat aaataccgct
    19201 gtttcctgaa cacataaagc tgatcaagca gatcataaag aagtaagcag cagcttgtga
    19261 accaaataat tcgattccca tcacaaaaca ggcaatcggt gtatttgtcg caccagaaaa
    19321 taccccgata aaacctaatc cagcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tactgcgcca agcgttgcac caatctcaaa tagtggtgtc acttctcccc cttggtaccc
    19441 cgcacctaaa gacaagaccg tgaagaataa tttcccaata aaatcataag catgtgcctg
    19501 tccattaaat gattcctgca gtaacggaag actcaaccca aggtaacgtt gtgtttgcaa
    19561 aatcaacgct gcggctacaa ctactgctcc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgc gcataccgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    19801 aaaaagcgca gcaaagaagc taggaaatat tgcttctgtt cggactttac cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataacca aaatctcccg ttctgcttta tccaatcgga ataaatgcgc
    19981 tatattatca gctaatgctc cccccatttg aactgctgtt ccttcacgac caacagatcc
    20041 cccaaataaa tgggtagtga tggtcccaaa caaggtcaat ggaatgagcc gcaaagggat
    20101 cttctcttct cctccattcc cttgatcgat cactaagtta ttcccacgac tagaaagacc
    20161 gccatgatac gcataaagat aagcaaaaag cgcgccgctg actggcaata gataaagtag
    20221 ccaaggatgt gctagtcgga tatctgtcac gaacgtcagg ctatttaaga aaaaagcaga
    20281 caagctcccc atgaatactc cgagaatcgc tgtgaccacc agccacttca acatgtacat
    20341 caaaatacgc actggactcg gtaattgttt ttccataaaa aattcctcct caaataaaaa
    20401 aacgcctact aaagaaaggg gctggcccca gactttagta ggcgtcatta gctatcttac
    20461 gttagcattt aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt
    20521 tctgtaaaaa acaataaaat tatcgttatt tctttacacg ttcagcaaat tcatagaaag
    20581 gagccaattt cgttaactgt tgattacatt gatcactatc atgacaaata taattcccct
    20641 ttttcgtata cgtgccatca gaaccggctt tcgttgtaga aagaaagagc gagacattcg
    20701 atgttttctg acagatcgca cagattccct tattaatcgt tggtgataaa gtaccgctga
    20761 tcccttttaa ttttccatct tcgtaataaa ccatgaattt tttttgcgtc cctggatcat
    20821 tccaaccgat gtacgtatgt gcttgcaagt cgatttcatt ccaagcgggt tgtttgagtt
    20881 ttttcacttt tcggaataat ttctcgattt gtggtttgct cggctgcata aacggctgga
    20941 cgatttgctt cagctcctcc aatgctcgtt ctgcttgcgt ttttgtcagt tttacatcta
    21001 aaactgtcgt taaaaacgta tcgatttctg atggttcgta acctaataca tcgcgaatct
    21061 tttcagctgc tagagcctga accgtttgta ccgtttttga gtcattgaca gatgagtacg
    21121 catttaataa ctgctctact tctcttttta taaaaaaata ttgatatggt ttcattgttt
    21181 gttccatgat tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga
    21241 tcttctgcaa agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg
    21301 catcttatca tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat
    21361 aaaagtaaac cgtatcttga gcagatagtc aactgcaaac agaaaaagga ctgtgacaaa
    21421 agtcgtgtca aagtcccttt ctacaaacaa acggtgttcc agaagtagct tcttcaaaaa
    21481 ataagccgga atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    21541 gaagctgacc cacttctgtc acaacctcaa tatggtctat tattggtttt acctgttaaa
    21601 tcgaggattc ttttccgaag taacgttgcc atctcttacg catacttgct gacataggct
    21661 gagcggcttc tttgactgcg cagtatttgt ctacgaatgt tacgatatat ccttctttgt
    21721 acttaggcgg tgcaatcgtt gcgccccaca tgtgcttcaa aatgatatct cgctcaagat
    21781 ccgacaaatc ggtgatcttt tctgcattct ttacagcgat tcttggatgg atatacgcat
    21841 gtgtgccttc atcaaatttc gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc
    21901 cagcacgcgc tgttgcttta gcattgccac cccatttttt tgcgagcaga taactatagt
    21961 aagaaacact gatggaatgc tccaaacgag tggaatgaac atgctgtgtg taattcgcta
    22021 attttttcac tgcttctgtt tctaaaagat cttctacata agacatatat tcttcgtctt
    22081 cacgccattt ttctgtcata attatcgatc tgccaccttt ttgaatttca ttttcttctg
    22141 aaattgatct aagtatacca ccagtgttcg gattattcta gtcgccttgg ataaaatagc
    22201 caaagcttaa tagttcctta atcataaaga aaaatgataa agaacaatcc ctgccgcaac
    22261 cccaacattc aacgattccg catttccttt catcgggata tatacgttct gatcagtcaa
    22321 agacaagatt tcctgactta ccccctgacc ttcgttcccc atgattattg cgaagttatc
    22381 tgatttttct actttgtcta aggcgtgtgc ttcttcattt aattctgtgc catacactgt
    22441 aaaccctttg tctttcatta aaggaatcac ttctgtcaaa tctttttgca acacaggcaa
    22501 atgataattg cttccctgca tgctgcgcag cacctttgta ctataaatat ctgccgttcc
    22561 tttgcctaat aaaacaccag ccatatcaaa tgcatctgcc gtacggatca tcgtccctac
    22621 atttccagga tcttgtacat tatctaataa cagccatcct cctgaaaaat ctggcgtgcc
    22681 ttcagccgtt aacgccatca ctgctaagat tccttgtggt gtaggtagat ccgatacagc
    22741 atccatgacc tcatccgaaa ctagaaaagt agaaatctct ttgtttgcgg ccagccagtc
    22801 tccccattct ttcatgcctc gctgtgtcac aaaaagttct tttatttctg catcagacga
    22861 cgcagcttct tcaatcagat ggaacccttc caaaagatat tcttcctttt cttcccgata
    22921 tttcttttta tgtagttttt ttaattcttt gattcttgga ttttttgctg attggatctc
    22981 tttcacattc ctcacctccc catgattata tcatggaata ggtgcttgag cttgtttcat
    23041 cctaaaaaaa tcggtatcat taatttataa aataggaaag aaaggtgacg aacatgcgca
    23101 aagttaaaat gaacgtccaa ggacgcgtcc aaggcgtagg atttcgctac atgaccaaga
    23161 tggtcgctga tcaactaggc gtgactggaa cggtaaaaaa cgaagatgac ggctcagtca
    23221 cgatcgaagc aatcggcaat gacgacatca tccagaaatt tattgaagaa gtcaagaaat
    23281 ctccgagccc aagtggacga gtgcagtatg tggatgtcca agaagacccg atgattgagg
    23341 aacgaaagaa atttgatgtg gtgggataag gttgtgaaaa agctggtctg catcaagcat
    23401 taaggacagg ttgtgaaaaa tagctctcca tatttttcaa caggctggac ttaatgccgt
    23461 agatgcaagc ttttgaacac cgtcaataag gttgtgagaa agtcgcttag ttttgagtag
    23521 taaggagaat tttgtgaaaa tggctttcca catttttgca taaaatttga cttaataccg
    23581 agaaactgac ttttgaacac cgtcgataag gttgtttttg agacgctttg cctcaagtat
    23641 taaagatagg tagtaaaaaa gctgatctat aacttgcaat aaatttgctt aacccatatt
    23701 ttccagacag aacaaaaaag tttttagttt tttaaataag aaagaaacac ttggaatcac
    23761 tcttttcttt ccaagtattt ctttcttgat tttttgtctt tttttcaaaa tttttcgcta
    23821 cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc catgcgcttt
    23881 tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat gaataaatgg
    23941 aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc tggctgtgtg
    24001 cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt actcgtccat
    24061 cctatgggcc aagcgattac atatctagtt gagaatttca attggtctta tggctgggca
    24121 gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat tagtcaatcg
    24181 aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt tgaagcagcc
    24241 caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca agcagaaatg
    24301 cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg tctgcctcta
    24361 ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac agaaggtatt
    24421 cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact tgttgtcatt
    24481 gcaggggtcg cctatctgct tcaaggatac atctcaacga tcggtattcc tgaagaacag
    24541 aaaaagacga tgcgtacgat gctaatcgtc tctcctgcta tgatcgtctt catgtcgatc
    24601 agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag ctgtctgcaa
    24661 acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc agaagagttg
    24721 aaacaaaacc cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga atcagcaaag
    24781 ccagctaaca acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac
    24841 aaaggccgca acgcaggaaa gcagcagaag cggaaataaa aacataggac aaaaaagatg
    24901 agagctaagc cctcatcttt tttgttactc ttcctcttct gctattccat taattaattc
    24961 gttgacagag acaatgcttt cacatcctgc acgaacaaac tcgggctctg aaccaatcat
    25021 ttgtgagttg ataaattcaa tgaccatcgg aagattcatc ccagcataaa gagtaatctg
    25081 ttgtccttcc attattagtt tagaaatgat atttgcaggt gtaccaccta aaagatcgca
    25141 tagtaccaca aaatcattta acgaatctgt gacagtgaga aacttttgtt tgtatgtttc
    25201 agtgccatct tctggtaata aaggtaacgt gtagatatcc tcttgaggcc ccatgatcat
    25261 ttctgtactt tttttcaatt cctcacaaaa ttttccgtga ctaatcaata ccaattgtct
    25321 actcattttt cgctctccct tatttcgaaa tcacacccaa tgcagataaa gcaacacaca
    25381 caattaatac gataaaaatc gctcgagtag gagtcatgtt tttctttcct aataaccaat
    25441 aaacaccacc gacgatccca gcaggtaaaa ttttaggtaa gatcatatcg atatagtttt
    25501 gcatattcaa tgtcacatcg ccaatcgttg gtgcccatga aaatttaaca ttgatcattg
    25561 tcgcaactaa tgcccctacc atgaataccc ctaataaagt ggctgcgtcc gttaatgcag
    25621 ttaagcgaga ttgcatagta gtaactaagg aaattccttc tttgtatgca aattcaagtt
    25681 gcttccaacg aaaaaccatg acaacgatct gagcaaaaat ccatataaac acacctatcg
    25741 gatttccatc agttgccata ttcgccgcca aagccccaaa aatcgttgga atcaaagcag
    25801 caaatacgga atctccaatc gctgcaaagg aacccatcaa tccagctttc agccccgata
    25861 cggtttcctt tgaagcaatt ccctcttttt cttccattgc aagatcaatc ccagtaatga
    25921 ttgtattaaa aaaattagaa gtattaaaaa attgcgtatg tgttttcatc atctctttta
    25981 attctggtgt attgtctcca tatatttttc ttagttgtgg caagatagta tataaatagc
    26041 cagaaccctg cattctctca tagttccaac ctaattgaaa agtgaacaaa cttcttcgat
    26101 tgatttgctt aaaatcttct tttgttaatt tgtaattaga tttcgtcatc ttcgatttcc
    26161 ccctcaacag aatgtgatgg ctcactagct aatggttgat ggtctttgtt ttgatcattc
    26221 ttataactga tcattgctag tgctagacca atcaatgcta cagcaagcat cggcagcgaa
    26281 ttgaaagttg ccggaagatc tttaacaacg cttgccacac ttgttcctaa taattgaaca
    26341 ttagagaata acgttgctaa taatgccgtg atcgtaaatc ctaaaataag ataagataga
    26401 tgttttttta ctggtaaata gcgcagaaga atggcaaacc caacagctgg taaaacagct
    26461 cctgctactg aaagtccatc gcctagccat tttagattcc cgtttaaaac agaaacaaca
    26521 ttttcaacta atccaccacc aaatgctaaa gctaaaaata caggaatcat acgagagagc
    26581 gaccaaggta aagctcccat taagaaattg cgttcgattg cttgataatt catgtcttct
    26641 acgtgacggt caatacgatg ggcaaagtat gtgttagcga atctagctaa aatatccaat
    26701 tggatcatta agcttgctac aggcactgca atagcagcaa tcgcttgttc tggattcatt
    26761 ccaagagaaa tcgagaaggc agttgctaaa atcgtcccag agttggcatc tatttttgat
    26821 gcgcctccaa acgtccccac tcctaaaact gtcaactgca tactgccacc aataatcaaa
    26881 cctgcgttta aatcacccat tactaaacct gcatataacc ctgcaaacac tggcgcactc
    26941 attgaagaat agatctgtaa ttcatctaaa atttgataac ctgcatataa tgttaataat
    27001 aagatctgcc accattgaat atccatggat cattcctcct atttgattaa aagattcata
    27061 aagtcttctg ctttatcgtt tggtaccatt tgagcaatta aatgtatacc aagctgatct
    27121 aattttttaa atacttcgat atctgcatct accacattga ttgattttgt aatggaccgt
    27181 gtttcattcg tttgtgacat attccccaca tttacttctt tgataggaac tcctttttct
    27241 actaaagata agattcgatc tggctttttt gctacgatca atactcgttg agagtcatat
    27301 ttgccagcta aaatatttgt tgctgctttt tcgattggca aaatgcttaa ttttacccct
    27361 gctggagtcg ctaacttcaa tccacttttt tcaattgtgt ttgtggctac ctcatcatcc
    27421 acgaccatga ttcgactaat attcagtttc gttgtccaaa gatttgctac ttatccatgt
    27481 atcaaacgtc catcgattct tacaccaata attgccatca tgcattctcc ttatctattt
    27541 tttctattta ttgttcaatt aaaactgtga catcatcaaa tatcccttct gtttcaaaga
    27601 taacgttttc attttttctt tcttatattt catttttaag ctaagaacaa attccttccc
    27661 aactaaagaa aacgctttct tttatattta agttcatttg attttcttaa cttgaatgtg
    27721 tttcatcaac atcgtaactt tcatagggtc gatccagcca gttttctctt gttgcgcgtt
    27781 tgccatacca caagccatag aaaatgcgag aatctcttcg attgattgcc tttttgataa
    27841 cgcatgggcc gatcctgcaa gggtcgcgtc acctgaacca acaggattaa caactgatat
    27901 tttaggaata atgacttcgt aaaaacactg agcaactttt gccacagcac ctctctctcc
    27961 taaagaaaca acaacccatg gcactccttc aaatattgga tgagctaata aggactgaat
    28021 tttttctaat tgaatcgaag aacagatacg ttggccaatc agttctccta attcttccag
    28081 attgggcttg ataagtagtg gcttctcctt accttccaat acttcaagca aaaatttacc
    28141 agaagtatct agtaatatgt ctttcccttt tttatcagct aaagcaacca attgcttata
    28201 gtaatcaaga gggcatcctt ggggtaaact acctgagatc gtcataatat ctgatatttc
    28261 gaggagctgc tcgaaatgat cggtaaatgt ctgttgttct ttctttgtta tgaaggggcc
    28321 tcgttccaaa agctctgttt gtctcgaact atgcaggata gcaatagaat cccttgtctc
    28381 tcctttaatt ggaacaaact gatgcatgat ccctaactga tctagctggt tctttagata
    28441 ttcaccgtgc tggccaccta ttaaaccagt agctatcacc tcttctccta ataaatttag
    28501 aaccctcgcc acattcactc cttttcctcc cgctgtttta ttgactgttt caactcgatt
    28561 gactttgtcg aggtttaatt cttcaattgt atacgcgata tcgatggaag gattcaaggt
    28621 aactgtcgta atcatttcat cttcaccttt ttgctttttg ttttattgaa ccaaggggta
    28681 gcggtttcat ttattacttt atttaaagtc tcgatgtttt ctctcccatt tgttcttaac
    28741 caatgaatag ctccgtcttt tccattttcg acgtatgctt tgaccccatc tttccatgtc
    28801 gcacgtccac acaatacacc attgaaggtg gaacccgatt catgagcaaa aatcagtgtt
    28861 tgttgaaata attctgttga aacaccccca ctcaaaaaaa tgaatggtaa tggagtcgct
    28921 tcactttgct ccttaaagaa ggcagcagcc tcttctttgc tgtgaataaa ttcacctgtg
    28981 gaatagccat tcacataatt catattgact ggtacttcta ctttcaacac atcaacttta
    29041 tattttggat cagaaaattc tttcatcatc tcaataactt tgtggggctt gactttcgca
    29101 tactctggag aagaactatc gcttatttgt gcgtcataag aaactaactc caaatagaag
    29161 ggacgatttt ctccttcgca ctcactacct aaacgttcga tgaacacatg cttcaaatga
    29221 ttgatttcgg gtgcttcatc aacatcgtag taaagtaaga attttactgc atccgcttgc
    29281 tgttctttca atctgagaga agaccaatcc gctaataaat caggtagacg accaggcgta
    29341 gttgcgtcat agccggtttt ctcataagct aacaataacc ccgtctcttt tgctctgact
    29401 tttgctgctg gcaatccata ttccggatcc aataaaatag cagaagcata gggtgtcagc
    29461 tcagctgata ctagttcttt gaattcactg atttggtctt cacttggctc ttttcccaac
    29521 gatttgatca ttttttttag agcaccacgc tgatcgatcg ctaatgcact aataatccca
    29581 tcttttgttg atagtctctc cattgcccta tttttttctt cattcatctg tcatttctcc
    29641 ttcggtttca tacttatgaa taatgacgcc cttgactact cgattgaccg tgcctgttgg
    29701 agatggtgta tctggtttat tagctacttt gattgaagta tagagtgcaa tgatttgtgc
    29761 agtcataatc atcgctaatg caagataggc atcgggcaat ttactcaccg agtcttccaa
    29821 tagcacagtc atacctgaga aatcaatagt acccggttgc gcaatcgcaa tgacattggc
    29881 agtgacccga tcttctttta tttcctctaa aatatctaag tcataatttc gcgtatatgg
    29941 ttgattgctc acaaaaccta caacgagtgt ctcgctattt aaaaaagact ttggtccatg
    30001 acgaaacccc atcgaagaat caaaaattgt agcaacttta cctgctgtta attctaaaat
    30061 ttttaattgt gcttctcttg tcgcacctgc caaacttcct gaacccagat aagcgatccg
    30121 ttcaaaaaaa tcaggtaata acttttgaat ttcttgatct cttgaaagaa gatcttgacc
    30181 gattttaact aattgttcaa gatatctttc ttttttctcc attgctgttt gatcaaagat
    30241 ccataaagca ctcaataaca tacacgtaaa acttcctgtc atagcaaaac ctgcatcgtt
    30301 tgctctctct ggcatcaata acagataatt ctgtttatca tcctgtgcgt tttttgccaa
    30361 catcccctct ttagcacaag taatcgtcaa atgaaacaat gtttcaactt gttcatttgc
    30421 aatttgcaca gccgctacac tttctggact gttgccactg cgagcaaatg aaactaataa
    30481 agtcggttca tttggaaaca aaaattcttt tggtgaagct acaatatctg tcgtgccaaa
    30541 actgtggaag ataaaaaact gattgtctcc atgttcattt agatagggta agagcgtatc
    30601 cccaatatat tgagacgttc ctgcacccgt aaaaataaca cgtattcttc ttccgtttgc
    30661 ttcttttttt atttgatcaa aaaatagagc taaattttct tggctttgca tcaatctatc
    30721 gattgtttct ttccagagat ctggttgttg ttttatttct tgtgtcgtga ttgctgcacc
    30781 tatttgtttt aatttgttct tcgatagctc aaacatgttg ttctccctct caatcatttt
    30841 cggatatgcc gaattttata atgaaactgg tccgctcttg ctacactatc tgtatattcg
    30901 ataatctcat ttttcacgtt ataggttatc ctcgtcagat gtaagacggg agcgccatca
    30961 gcgattcgta gcacaacagc atcttttttt tgcacgatac ttgcataaaa ctcttcatca
    31021 gctaattgaa cttgttgttc ataatcctgt gaaaacacat catacaacgg tttactttca
    31081 agtaacggca aatcaagtcc cataaatagt ttcgccggta gataagaacg ctcaaccatc
    31141 aatggctctt catctgccaa acgcaatctt ttaagtttaa atacagtttc acctaaatgg
    31201 atgttcaaat tcttagcaat tctcttatta gcttccattt gtctaaattc taaaatgacc
    31261 gtcttaggga cgcgccctat ttcgcgcatt tcttctgtaa agctatatgc tccagctaag
    31321 ttcgatgact tatcagacaa atcagagaca taggtacctt ttccatgtct tcggtaaata
    31381 taaccttgat tttccaattc ctgtaaagcc agacgtacgg tggtacgact caccccatag
    31441 tgttgacaaa gttctctttc agataacagc ttatcatgcg gagtcatctg tgtttcgata
    31501 cgatccttca ataaatcaac tagttgataa tacaatgatt ttcctttatt tgagctctcc
    31561 ataaaaatca cctcttcttt actggttata accacaacac aagaatagtg aattattaat
    31621 catttgtcaa aggtttttta gaccaaaaaa gctattataa cagctcttta aagctttata
    31681 tcttgaatag acaaagatac ctcaaaaata ccagttacca ataaaagaac agaaaaaaag
    31741 acgaaccaca taagcgatcc gtcaaaatgg tctaacttat cgagcagcgt gatccacatt
    31801 aatccctatg atattcctac aactatcgtt ttattacttc caatatcatt tacaacaact
    31861 aaaaatagct tattaatgac aaatgttatt attagtttta gataactttt gcagttttat
    31921 actttttttc tataaaattt gacaaacatt tttttaaata actaattatt ttttgaatct
    31981 gtcactcaga caatcctttc tcttacttgt taacataaat agaagttgct gtttcttcta
    32041 atagtgtact taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt
    32101 tttcttcgcc ttcggtaaca aaaggtgcta ctcccttcgc ccatgttgct cgcccacata
    32161 agacaccatt gaatgtcgaa cctgattgtt ttgcaaaacg caatgtatct tgaaatagtg
    32221 cagcgctgac tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat
    32281 agaaatacgc ggatgcctct gcttgactat aacaagtatc ttgcccatat ccttccacaa
    32341 atctcatatt cactggtact tctactttca atacatccac ttgataacgt tcttttgaaa
    32401 attctcgcat ggcttcaatc actttatgcg gttttacttt tgcatagtca agagaagacg
    32461 gatcgccaat agttgcatcg taagaaagga gctctaaaaa gaaaggtatg ccttctgctt
    32521 cgcattctga accaatccgt tctataacta tttgtttttg cacattaatc tctactggtt
    32581 catcaacgtc atagtacaat aaaaatttac aagcatccgc acccacttct tttaaccttt
    32641 tcactgacca actattcagt aaatctggta gtcggcctgg cattttcgca tcatagccgg
    32701 ttttttcata agctagcaac aaaccacatt ctttctctcg aagcgctgct gcaggcagac
    32761 catattctgg atcgagtaaa atagcggaag catacggagt caggtactga gaaaccatct
    32821 gtttaaattg gatgatatct tctgatgtag cttctttata ttggttgatc attttcttca
    32881 aagcaccacg ttggtcgata gctagcgcgc taataattcc agcttcatcc gtcaatcgct
    32941 tcaaacgttc ctgtttttct atactcattt tcttcatata tcttcaccta tttctttagt
    33001 taatattgta gctatttcta tttttgattt tgcagctctc aactgttctg taacctcttt
    33061 tcgcatcaat gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga
    33121 atctgggata aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc
    33181 aattcctttt ttcagtgtga cgataatcaa actaggttca ttgattgttt gagacttcgc
    33241 atgagggata gcaaaacctg acattattcc tgtagtacct tctttttctc tttccagaag
    33301 tttttgatat acttcttttt cgtcatgagc gattcctaaa cagaatgttt gctttgctaa
    33361 aaacttaaat gcttcttctt gattatctaa tggctgttgt aaaaaaatgt tctcttcatg
    33421 aatgattgtc atgataaacc tcctgctctt ccttcttcga taactgtacg atttgttgct
    33481 ttattatccc tcttcttgtc tttatcagtt actcttttct tttggatcaa acctagtatc
    33541 actccaccaa cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact
    33601 acaggtaaca ctaaaaaacc gccatgtggg gctggtactt ggacaccaaa cagataggtc
    33661 aagacagcag caattgatga gcctaacatc atcgttggta atactcggat cgggtctttc
    33721 gcagcaaacg gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa
    33781 cctgcattac gatcattagt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta
    33841 atcaacggtg gtgcaatgca agctgctgac gcaccagcca taaaagtcgt gtttccttgt
    33901 gtcaaaagag ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac
    33961 gcacacatac agccaacaat cacgccaaga acaagtggat tcgaatattc aaatccagct
    34021 aaaaagctca tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg
    34081 atgccaataa atgctacacc aataactgga tataagacga ttgccttcaa gccatctagt
    34141 tgtttaggta aaattatcag tagcttttgc aaaaataaga taaaataacc tgctaagaac
    34201 ccagctaaaa tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca
    34261 aatccaacga ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact
    34321 ggaaccatca ttcccattgc tgcatcgtca actgctttta ccattgccgc tgtagcattg
    34381 tattgtgcat gatctggatc agaagaatag attccccaaa cggcaaatga aatagcaatc
    34441 aatactccgc cagctaccac gaaaggcaac atatgagaga caccgttcat caaattttta
    34501 tagatgctac gaccaaaatt agcaccactt atcgcttctg gttctaaagt atcagcagct
    34561 tcattatctg ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca
    34621 tccttgattc ctgtactgac agaaacatca atgactcgtt ttcccacaaa tctttctgaa
    34681 tggacatctt tgtctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt
    34741 aattcattct cgatcccaat ttgtccatgg gtctccactt tgatcgtgat gccttttttc
    34801 ctagctgctt gctctaatgc ctcttgcgcc atgtaggtgt gcgcaattcc tgtcggacaa
    34861 ccggttgctg caattagttg atattttccc atctttcgaa cctctcttcc ttgattttga
    34921 tttgtttact taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcagtag
    34981 agctgcctgc tgctatgctt ttacgtaacg tttcatcaag cgatcgtcga ttcatgtatc
    35041 ctgctaaaaa agtgcctaac atggcatcac ctgcacatgc tgtattgaca acttttcctt
    35101 ttggcgagtt tcctcgaatc accctgtctt tcgtaaaaag aatcgctcct ttctcaccga
    35161 gggataacaa aacattttct gcgcctgact ctactagtcg ttgtccataa acaaagtaat
    35221 cttcatctgt aaccattttt cgtccgaacc aagaagaaag ttcttcctca tttggtttca
    35281 gcaaaaacgg tcgatagggt aaacaatcca tgacttcttg atcactacta tcaataatca
    35341 aacaaatccg actgatttct attaaaacct ttggagacag ccctcttggt aaactcccag
    35401 aaacacaaag ataatcccct gcttgtaatg ctcttatttg atttaaaaaa ttatgaacag
    35461 ctgtctgcgg tatttcaggt ccttgattca ctaatttgta ttcttctccc gtttgattga
    35521 cttgtgtaaa tacattgata cgcgtcatac caggcacttc aataaactct gtttggattt
    35581 gtttctgctg taaatagtct tctatatact ttcctgtgaa cccagcagaa aaacctaatg
    35641 ccgtacttgg gaaatctagc atctttaata ctagtgacac attcacccct tttccgtttg
    35701 cctgaatgtc atcctctttt gtgcggttta ccataaaagg atgcatctca tctgtttcaa
    35761 taaatagatc aattgccaaa ttcatcgtgc atgtgtagat agacataacc aacgacctcc
    35821 ttgattctat tatgaaatac gcgaaataga aaatgttttc ttaatcgtcc caacacaaag
    35881 aaaaccttta catttataaa ctcgtcgtgc tttttttaac taacttttgg tgaaagaagg
    35941 aaagaatatg aatcacaaat taagtgaaac agagcagtac ctctggaact ttatcgaaca
    36001 cctcatccta gagatcccta actattccat cgtgaaacta agtgaacaag caaatgtctc
    36061 aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaagggttta cttcgtttaa
    36121 gcatcatcta aaagaaggag aaaacaagaa catcaatttt tcatttttag ataaagtaga
    36181 caaaggaatc cgccgagcca ttctaaaaaa tgaacaagaa gtggtacgta caatcaatat
    36241 gttggaaatc gggaacattg aagatgcaat acaaagaatc aaatttgctg accgggtttt
    36301 tgtctttgcc cgaggatttt cagaaatgat tggccaagaa atgctggtaa aacttcaatt
    36361 aacgggaaaa aattgtcaga tgcatactga tccagaaatt atcaaaagca tcagtcggaa
    36421 actaactaag aaagacagcg ttatttttgt ttctttgaac ggtgagaaaa caaataaaaa
    36481 catcaaaaat aagatttatg tctttatttt tcaatttttt aaat
//
LOCUS       DAZKAY010000017        35882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000017 DAZKAY010000000
VERSION     DAZKAY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.017"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8WP_000779"
     CDS             <1..1152
                     /locus_tag="ACG8WP_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFP9027108.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8WP_000780"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8WP_000780"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9027109.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8WP_000781"
     CDS             2728..3003
                     /locus_tag="ACG8WP_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9027110.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8WP_000782"
     CDS             3009..3776
                     /locus_tag="ACG8WP_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9027111.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8WP_000783"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8WP_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9027112.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8WP_000784"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8WP_000784"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9027113.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8WP_000785"
     CDS             7920..8222
                     /locus_tag="ACG8WP_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9027114.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8WP_000786"
     CDS             8225..9388
                     /locus_tag="ACG8WP_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9027115.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8WP_000787"
     CDS             9414..12842
                     /locus_tag="ACG8WP_000787"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9027116.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8WP_000788"
     CDS             12863..13987
                     /locus_tag="ACG8WP_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9027117.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8WP_000789"
     CDS             13968..14414
                     /locus_tag="ACG8WP_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9027118.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8WP_000790"
     CDS             14398..14736
                     /locus_tag="ACG8WP_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9027119.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8WP_000791"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8WP_000791"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9027120.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8WP_000792"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8WP_000792"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9027121.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8WP_000793"
     CDS             15893..16936
                     /locus_tag="ACG8WP_000793"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9027122.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8WP_000794"
     CDS             17095..17823
                     /locus_tag="ACG8WP_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9027123.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8WP_000795"
     CDS             17939..18184
                     /locus_tag="ACG8WP_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9027124.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8WP_000796"
     CDS             18181..18399
                     /locus_tag="ACG8WP_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9027125.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8WP_000797"
     CDS             18535..19074
                     /locus_tag="ACG8WP_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9027126.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8WP_000798"
     CDS             19067..21340
                     /locus_tag="ACG8WP_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9027127.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8WP_000799"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8WP_000799"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9027128.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8WP_000800"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8WP_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9027129.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8WP_000801"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8WP_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027130.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8WP_000802"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8WP_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027131.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8WP_000803"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8WP_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9027132.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG8WP_000804"
     CDS             complement(24499..26427)
                     /locus_tag="ACG8WP_000804"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9027133.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG8WP_000805"
     CDS             complement(26624..27457)
                     /locus_tag="ACG8WP_000805"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9027134.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG8WP_000806"
     CDS             complement(27649..28353)
                     /locus_tag="ACG8WP_000806"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9027135.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG8WP_000807"
     CDS             complement(28376..29002)
                     /locus_tag="ACG8WP_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9027136.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG8WP_000808"
     CDS             29328..31703
                     /locus_tag="ACG8WP_000808"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9027137.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG8WP_000809"
     CDS             complement(31727..32218)
                     /locus_tag="ACG8WP_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9027138.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG8WP_000810"
     CDS             32405..33325
                     /locus_tag="ACG8WP_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9027139.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACG8WP_000811"
     CDS             33479..33823
                     /locus_tag="ACG8WP_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9027140.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACG8WP_000812"
     CDS             complement(34013..34513)
                     /locus_tag="ACG8WP_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027141.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACG8WP_000813"
     tRNA            35041..35124
                     /locus_tag="ACG8WP_000813"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACG8WP_000814"
     CDS             complement(35448..>35882)
                     /locus_tag="ACG8WP_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9027142.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAZKAY010000018        35563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000018 DAZKAY010000000
VERSION     DAZKAY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.018"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..207
                     /locus_tag="ACG8WP_000815"
     CDS             <1..207
                     /locus_tag="ACG8WP_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9027143.1"
                     /translation="AILAALMALSLISRQILYYSFCDVNKHSNYEIGQLIRGYGEKNV
                     EKLKSIALIEFAEAYKKGVLIQYR"
     gene            922..1089
                     /locus_tag="ACG8WP_000816"
     CDS             922..1089
                     /locus_tag="ACG8WP_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027144.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            1115..1459
                     /locus_tag="ACG8WP_000817"
     CDS             1115..1459
                     /locus_tag="ACG8WP_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9027145.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1464..1745
                     /locus_tag="ACG8WP_000818"
     CDS             1464..1745
                     /locus_tag="ACG8WP_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027146.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1848..2162
                     /locus_tag="ACG8WP_000819"
     CDS             1848..2162
                     /locus_tag="ACG8WP_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9027147.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2140..3834
                     /locus_tag="ACG8WP_000820"
     CDS             2140..3834
                     /locus_tag="ACG8WP_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9027148.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3854..5032
                     /locus_tag="ACG8WP_000821"
     CDS             3854..5032
                     /locus_tag="ACG8WP_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9027149.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4995..5681
                     /locus_tag="ACG8WP_000822"
     CDS             4995..5681
                     /locus_tag="ACG8WP_000822"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9027150.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5681..6841
                     /locus_tag="ACG8WP_000823"
     CDS             5681..6841
                     /locus_tag="ACG8WP_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9027151.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6851..7726
                     /locus_tag="ACG8WP_000824"
     CDS             6851..7726
                     /locus_tag="ACG8WP_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027152.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7723..8034
                     /locus_tag="ACG8WP_000825"
     CDS             7723..8034
                     /locus_tag="ACG8WP_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027153.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8024..8377
                     /locus_tag="ACG8WP_000826"
     CDS             8024..8377
                     /locus_tag="ACG8WP_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9027154.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8367..8768
                     /locus_tag="ACG8WP_000827"
     CDS             8367..8768
                     /locus_tag="ACG8WP_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027155.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8761..9165
                     /locus_tag="ACG8WP_000828"
     CDS             8761..9165
                     /locus_tag="ACG8WP_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027156.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9177..9785
                     /locus_tag="ACG8WP_000829"
     CDS             9177..9785
                     /locus_tag="ACG8WP_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9027157.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9805..10167
                     /locus_tag="ACG8WP_000830"
     CDS             9805..10167
                     /locus_tag="ACG8WP_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027158.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10170..10352
                     /locus_tag="ACG8WP_000831"
     CDS             10170..10352
                     /locus_tag="ACG8WP_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027159.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10370..13702
                     /locus_tag="ACG8WP_000832"
     CDS             10370..13702
                     /locus_tag="ACG8WP_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9027160.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            13753..14490
                     /locus_tag="ACG8WP_000833"
     CDS             13753..14490
                     /locus_tag="ACG8WP_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9027161.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14500..16791
                     /locus_tag="ACG8WP_000834"
     CDS             14500..16791
                     /locus_tag="ACG8WP_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9027162.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            16815..18896
                     /locus_tag="ACG8WP_000835"
     CDS             16815..18896
                     /locus_tag="ACG8WP_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027163.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
     gene            18910..19257
                     /locus_tag="ACG8WP_000836"
     CDS             18910..19257
                     /locus_tag="ACG8WP_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027164.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            19250..19390
                     /locus_tag="ACG8WP_000837"
     CDS             19250..19390
                     /locus_tag="ACG8WP_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9027165.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            19460..19879
                     /locus_tag="ACG8WP_000838"
     CDS             19460..19879
                     /locus_tag="ACG8WP_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFP9027166.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            19876..20886
                     /locus_tag="ACG8WP_000839"
     CDS             19876..20886
                     /locus_tag="ACG8WP_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9027167.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            21076..21149
                     /locus_tag="ACG8WP_000840"
     tRNA            21076..21149
                     /locus_tag="ACG8WP_000840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21110..21112,aa:Ile,seq:cat)
     gene            complement(21663..21968)
                     /locus_tag="ACG8WP_000841"
     CDS             complement(21663..21968)
                     /locus_tag="ACG8WP_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9027168.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(22495..22567)
                     /locus_tag="ACG8WP_000842"
     tRNA            complement(22495..22567)
                     /locus_tag="ACG8WP_000842"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22532..22534),aa:Lys,seq:ctt)
     gene            22948..23517
                     /locus_tag="ACG8WP_000843"
     CDS             22948..23517
                     /locus_tag="ACG8WP_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9027169.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            23504..24364
                     /locus_tag="ACG8WP_000844"
     CDS             23504..24364
                     /locus_tag="ACG8WP_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9027170.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            24554..25258
                     /gene="yycF"
                     /locus_tag="ACG8WP_000845"
     CDS             24554..25258
                     /gene="yycF"
                     /locus_tag="ACG8WP_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9027171.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            25263..27098
                     /gene="walK"
                     /locus_tag="ACG8WP_000846"
     CDS             25263..27098
                     /gene="walK"
                     /locus_tag="ACG8WP_000846"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9027172.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            27095..28411
                     /gene="yycH"
                     /locus_tag="ACG8WP_000847"
     CDS             27095..28411
                     /gene="yycH"
                     /locus_tag="ACG8WP_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9027173.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            28412..29281
                     /locus_tag="ACG8WP_000848"
     CDS             28412..29281
                     /locus_tag="ACG8WP_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9027174.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            29336..30145
                     /locus_tag="ACG8WP_000849"
     CDS             29336..30145
                     /locus_tag="ACG8WP_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9027175.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(30248..30877)
                     /locus_tag="ACG8WP_000850"
     CDS             complement(30248..30877)
                     /locus_tag="ACG8WP_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9027176.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            31047..32339
                     /locus_tag="ACG8WP_000851"
     CDS             31047..32339
                     /locus_tag="ACG8WP_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9027177.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(32415..33077)
                     /locus_tag="ACG8WP_000852"
     CDS             complement(32415..33077)
                     /locus_tag="ACG8WP_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9027178.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            33302..33586
                     /gene="groES"
                     /locus_tag="ACG8WP_000853"
     CDS             33302..33586
                     /gene="groES"
                     /locus_tag="ACG8WP_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9027179.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            33637..35262
                     /gene="groL"
                     /locus_tag="ACG8WP_000854"
     CDS             33637..35262
                     /gene="groL"
                     /locus_tag="ACG8WP_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9027180.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12285 a         5638 c         7519 g        10121 t
ORIGIN      
        1 gcgattctag cggctttgat ggctcttagt ctgattagtc gtcagatact ctactacagc
       61 ttctgcgacg taaataagca ttctaattat gaaataggtc aattgatacg aggatacgga
      121 gagaagaatg tagagaagtt gaaatccatc gcattgattg aatttgctga agcatataaa
      181 aaaggcgtat taattcagta tcgttgattt tgtagggttt ttgtagggat agtgtagggt
      241 ttttgagcgg tttaacgtga tattatggta gtgtcgaaag attaggaaac aggacttcga
      301 caaaataaaa tgtaagggag gaaatctccc tcatcgttta attaagcttc gatagacagc
      361 agcggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc
      421 tatcatccgt tgctgtctat tgtcattatg tcactgtggc ggaaagggta gacgcttaaa
      481 aataaggtca atacgtcgag ggatagcctt aacgttttat gatttgacca tgcaatgttc
      541 gattcattgc cagtgactta aggaacctac ggaaacaatt catcttatcg gatgccgatg
      601 aattggctga ctagtcggga tgctactagc agttagaagg cataaaatac tagcgcagac
      661 gtgcgccact ctcaggtgta ggttaggaga gaaacattag ttggggttat taggaatacg
      721 ataacctgct tgcgacaaag ctttgtactg tcgcgttggt catgaacaga gacggtattc
      781 tgtttcagta ttcgttagca accgagggat gtggcagtgg tgaggtgcag gaagtatcag
      841 acttgtttgt gtgcaagtta ctattacata ttagatcact ctttgagtgg tctttttatt
      901 ttgcgtaaag gaggcacgac gatgtataga ccacaatact tagaacagaa gcatgaagta
      961 atcactgtgc aaaacggtaa cggtgagata gtacgaaagt atagaagacc aataaagagc
     1021 gatacatata aacggaagga aagcaatgaa gttattccat tgtatggcaa aagaatagct
     1081 aagtattaaa taagattgcg aaaggagatg ggacatgacc gaggaattct atagatggtt
     1141 attacagttg ataagagaag atcgtttggt taagttctat cagtctccta aatggcgcag
     1201 gcttagagag aaagcgatga aacgagatca ctatgaatgc caagagtgta gaagactagg
     1261 taagtatcat agagtagaga acgttcatca tataaaggaa gtcaaggata gacctgactt
     1321 agctttagat ttagataatc ttatttgttt atgtgttgaa catcataatg aagttcatgg
     1381 cagatatctt acagcgttag ataaacaaga gaagaagata gaaagcttcg ctaacttcga
     1441 tgcaagtgaa aggtggtaag tgcatgatca tcaatgacaa tggcagagag tatgatacag
     1501 aaaagattga agagtattca tcttatactc agggattaat taaacgtttg atatacgttc
     1561 gctatgtagg tattagggat ctgttatcag ataactgttg tagtaaatac aaagtgaatc
     1621 aagtaagaga agcgttgaat aaagataata acgtcgaaag aataaaaaat gtttttggat
     1681 atagtattga agagattaat tattacattg acttcgctga agctttcatt ccgatggtga
     1741 gataaccccc ccttaaaata aatcgcaaat tttttggggg tgatgaaacg gagggggctg
     1801 tcaggaaaag agattttttc gaactttatc atgaaaggag ggctaaaatg tttaaaaacg
     1861 aattgtctca aaatcgctac agagaaaaat tacgccgctc tttaataagc caattggaaa
     1921 gtcagaaaac aaatattgag ccattcttag ataatgttga tcgttatatc agtttatggg
     1981 aaacggcgat atcactggaa gaagatatat ccgagaacgg cattagactg gagaatggta
     2041 aaaagaatga atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg
     2101 ataaacttgc cattactcct gaattggtag gtgaagcaaa tgaatcaatt cctgagttat
     2161 aagcatattg aaaattggtt caaagctata gaagaaggca ctatcaaggt atgcaaagag
     2221 caattattgc taaaaaatta tctagaagaa agagtcttta ctagagaaga tatttacttc
     2281 gataagcaga tggtagagga ttcaatcaat ataccagcac aatactttcc attcgaatta
     2341 attccgtggg aaaaatttct acaatgtttt atttatggtg ttcgatggaa aaaagataaa
     2401 acactagtgt tcaatagata tctttcatta atgggacgtg gtaatggtaa aactggtttt
     2461 gcttcttgga acaacttctt tctactaacc gctaaacacg gtattaaaaa ttatgatatt
     2521 gatatctatg ccaataatga aagccaagca aagactagtt ttgatgatgt atttaaagta
     2581 attaaagatc atcctgattt agataaaaaa gtatttaaag ctacgaagga agttattcaa
     2641 aatatcgcta caaacagcaa acttcgttat aacacggcaa atgctagaac aaaagatggg
     2701 aagcgaccag gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta ttcaatgata
     2761 aatgtggcta cttctggtgg tggtaaaaat cgagattata gagaatttta tgatacaact
     2821 aatggtcatg ttcgtggtgg tccgcttgat gacattatag aagaatcaaa aatgattctt
     2881 tctggagaac ttggaattga caaggatgga gcagaatttt ctagtttgtt tccatttatt
     2941 tgtcgcttgg ataacgataa tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca
     3001 actattaatt acaatgcaaa tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa
     3061 cgtaatgctg gtttaagact tacgttcatg accaaacgaa tgaacagacc tatggaagat
     3121 acacgatttg ctgttgcttc atatgatgat gttctgcata cgaaagaaaa agaatttcct
     3181 gaaaaaatgg atgaagtgat aggaacagtc gattttgctg atagacgaga ttttgccagc
     3241 gttgggttgc taggaaaata cgataaagat gtttatttta cacaacatac ttttatccac
     3301 gaatcagccc ttcgattaca aaacatcaaa cgagaggtta tagatatttc tatagatcaa
     3361 ggaaaatcac agatcgttca tggaaaaaat atagaagctg attatattgt aggttggttt
     3421 cttgaaatga gtaataaata ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa
     3481 atattgaagc ccgctttaga agaagcaggt tttactgtgg aaattgttcg aagcggatct
     3541 gttacacatg gtatgttaaa agatctggtt gatgaccttt ttattaatca acgtttattt
     3601 tttggtgacg atgcgattat gcgttggtat tgcatgaatg tatatgaaga gcatatttct
     3661 aatggaaata tacgctatga aaaaatagaa cctgaaacta gaaaaacgga tggctttttt
     3721 tcattccttc atggtttgaa ttttttagat gatatttatg attctgctcc tgtaacagtc
     3781 acaaatagct cagtagaaaa tacaggaact ggatttactc ctctagtatt ctaacttgaa
     3841 aggaggtgag aaagtgggga tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac
     3901 ggttacttta gaagaatact tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc
     3961 gattgaaact tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata
     4021 tgaagatgga aaaaataaaa agaatgatct ttactatagg ctgaatgtag ctcctaataa
     4081 gaaaaataat gcaacagaat ttagaaaaaa actgatcagg agattaatat tctacaatga
     4141 agtattgatc gtttctccgt ctaataattc tagcgaaata tttattgcgg atagttggga
     4201 tgtcacagaa tatgcattga aagatgatgt gttttctcaa gtgcaaatta acaacatagt
     4261 ccttgataga gaatttctag aaagtgatgt tatctatata aaatacgcag atcaacaaat
     4321 taggcaacta gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta gtgccatgaa
     4381 tgtttacaag cgctctaacg ctcgtagata cgtactgaaa gggaatttat tccgaccgca
     4441 agacaataca acacaagatc aaatcaataa aatgatgaca tcacaattta aggcttttat
     4501 ggaagctgat aatgcaggtg cggtatttca attacaaaat gagtacacat tagaagattt
     4561 cagcggaaac ttccaaagca attcaagaga tataaaaaac ttaatagacg acatctttga
     4621 gatgacagca gcagcgtttc acgttccgaa aaacctacta aagggagaca tgagtgggtt
     4681 atcggatcaa gtggacgctt ttttaatgtt cgaaatcata ccgattgctg aacttattca
     4741 ggatgcgttt aacgctagtc tctatgaagt agaagaatac ttgtcaggga attttgtacg
     4801 tgtggataca actatgatca agattactag cttcaaagat ttggttgacg ctattgatgt
     4861 aggcattaga aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg
     4921 ctctgataag gcgatggcag atgaaatatt tataactaaa aacaatcaac aagtatcgaa
     4981 aggaggtgag gcgaatgacg acaatgaaaa catttctagc agtaaagaat gaaggcgcag
     5041 taccgcaaat ttttattcag ggatttattg gttctagttg gttctttgaa gggaatactg
     5101 acaagggaat caaaaatatt ttggatagtc taggtgatca agaagaaatt gaagtagtaa
     5161 ttaattcaaa cggtggagac gtatttcaag ggattgctat tgggaactta cttaagtcaa
     5221 ataaagcaaa agttaacgtt gtgattaacg gcttagccgc tagtgctgct tcaattatcg
     5281 caatggctgg cgatactata aaaatctaca acaatgcaca attgatgatt caccgcgctt
     5341 ccacatacgg agaaggaaat gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa
     5401 ttgataaatc ggtaaaggct tcatataaaa cacgattcaa tggcacagat gaagcattgc
     5461 aagaacttct tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt tatggattgg
     5521 tcgatgaaat tatcgatgca gaaaatagct caggtactga agctaaaaaa gaacaaagcg
     5581 ttgaagaaat tttgaatgac gttaaagaaa aaagagcaga aaaaattgct gcatttacag
     5641 cagcattaaa taaaacattt ggacaaggag atgtaaaata atgacagtta aaaatttaaa
     5701 aggtgtaaca gctgcaagcg accaattgat gaaagctttt aaagatggta acgaagaatc
     5761 ttttagcgca gctatggtaa gcttatctaa ggaaattcag gataaaattt tagaagaagc
     5821 aacagcaaaa aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg tattaactac
     5881 acaagaaaca aaattctata acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga
     5941 attagtgcca gctactgtat ttgaacgtgt atttgaagat ttagaacaat ctcatccact
     6001 attgcaaaaa attacttttg ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg
     6061 agtcaatcca gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt tagataatgg
     6121 ctttgatgta attaacatga agcagttcaa gctatcaggt tatattcctg tatgtaaggc
     6181 aatgcttgat ttaggtccag tatggttaga tcgttatgtc cgtactgttt tagtagaatc
     6241 attgagaatt gcattagaac aagcaattgt tgatggtact ggtaaagata tgccagtcgg
     6301 aatgatgcgt gacatgagca aacaaactag cggagaatat gctgaaaaaa aagcagaacc
     6361 tattacagct ttagatgctg taactatggg cggtttgatg gcgcgactat caaaattcaa
     6421 tatcgaaggt gtgaatgatc cgatttatcg taatgtgaat ccttctgatg tggtcctaat
     6481 tgtgaatcca actgattact ggtctaaagt attcccagct aagactgtac taactgctaa
     6541 tggagaatac gtacaagtat tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc
     6601 agaaggaaaa gcagttattg gggtagcctc agattacttc atgggtgtag gatctacact
     6661 aaaaattgaa gcttcagatg aataccattt tgttgaagac gaacgcattt atctagctaa
     6721 acaatatgca aacggtcaac ctaaacgtaa cgatagtttc attgtattag atattagcgc
     6781 tttgggaact actactacaa ctacaaaacc aacaaccaca acaactacaa cacaagcgta
     6841 ggtgatcaga atgaagtata ttctttgtca gccggcaatc aatcggttta aatgggagct
     6901 tgaagtttgt ttaactaatc tgaagaaact aggaatcaaa gatatcgtat tgcttttcag
     6961 cagacacgat gatcagattc ctattttttt tgagaaggaa tatggtgttg aagttcatgt
     7021 gtacgatgat ctgcgggacg acaaagagta tattccttcg attaaaccat atttatggtg
     7081 gaaatattta gaagaagatc attcgcgtga ggacgaccga tatttctata tcgattcgaa
     7141 tgtcattttc aataaaagaa ttaatttgcg caaattgcct tctaaagatg atgtttggta
     7201 ttgtagcgac tgctgtagtt atctaagtct tgattatatt agaagctgtg aaaacggaga
     7261 aaatattcta aaagatatgg caaacattgt aaatgttaca gtagaatctt tggaaactat
     7321 aaacactaat tcaggaggcg cacagtgggt tattaaccgt cctaaagcga attattggaa
     7381 aaaggtttat ctggattcta atcggctata tcgctacctt agagggcaaa aaacaaatat
     7441 acaaatctgg acagccgaga tgtgggcaca gctttggaac atgatgtatt tcaatattgg
     7501 tcctaaagtt cacgaggaat tagacttttg ttttgctact gatccaatag aaaaagttaa
     7561 agaagtaaaa atcttacaca atgctggagt aacaacaaac gatgaagatt tatttttcaa
     7621 agggagatac gtgacttcta cgccttttga tgaagattta tcatttgtaa acaagaaaaa
     7681 atgctcttac gcatatgtta aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg
     7741 actgaagaat tgctaatagc tgtgaaggat aatatttacg ttacctggaa cgaagaagat
     7801 gagtcaatta aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca
     7861 acactttctt tttctcctga aagcttagaa tacggattgc taatcgaaag atgtagatac
     7921 gactggaatc gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt attaggtttc
     7981 attcaacatt atgcgctaca agaatatatt gcaggtgatg tgaatggcga ataatcgtag
     8041 actcgaagaa acattcaacg atggttggtt aaagattttg acgcaaacta ccaaaagaaa
     8101 tgaactagga aaaaagattg gtgtagaaga tacagaaatc acttctttaa aatttagaaa
     8161 tctttccatg agagatagtg atataacagc tatggatgcg atgggatcga aattaactaa
     8221 gaaagtaaag actccatttc atccaatcgc caagaaattt aataaagatc aatattttat
     8281 cgtaatcgat agtatgcgtt acaacgttat ctatgccgat tacgataatt tttatatcta
     8341 tttttatctt gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt
     8401 taaataagca aatttctgct atcaaaactt ccttagaaga gcattttggc ctcaaactct
     8461 ttcaagactc cgtaggcgag gatgagctac ctgatgattt taattacttc attctcgaaa
     8521 caggagaaat agaaatgatc actgagccaa aatatagcgt gggtcaaaat ctatatctaa
     8581 ctttctattc agaaaataga gaagatttaa caggagactc actagatatt atttcattga
     8641 ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa ctagagaacc
     8701 aagatcgcta tatcgatcaa ttggtattta cgtttagacg attattgaag agtgattgtc
     8761 atggctaaaa atagttggga gctaaaaata aatggacatg atgaacttct tgtgcggatg
     8821 gaacgctatt caagcgagag cgaacgactg attaacgaag cattgaaatc aaaaggttcg
     8881 gctattgcag tggataggat tacagaaaaa attcctgttt ctgaagcaga tttaagaaga
     8941 gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc aatacattaa tttgggtttc
     9001 atcattagac ctacaagaaa atttgagtat ttaaaatatc ctgatttggg gataggtact
     9061 tctaaaagaa atcagccgga cgaatttatg agaagaggat taggtcttgc acttgatcca
     9121 attacagaac ttctgattcg tcaattcgat aaattaaata aataggggga acaacaatgg
     9181 ctaaaacaaa aactgtagta acaacgttcg ataacataag tatcaaacga attgctttta
     9241 attttaagaa cgcaggaaat gcaatcgtaa cgaattgtaa tggacaatta gatggcgaaa
     9301 cagaaatgca aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac
     9361 caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgtttca
     9421 atgggttgaa acaagatgga cgtattaaac caggcattta ctcttacggt cctgattccg
     9481 taggcgaaga tttctcactt gctgcagaga tcgtggatga cttcgaagaa aatagaaagt
     9541 tagttggtat gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg
     9601 cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat
     9661 tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga
     9721 atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac
     9781 agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat
     9841 ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga
     9901 gattttaaca cagacgagtt tgcaaaaatg tcgatgggaa aaggctctat ggatattaca
     9961 gttattcaag gtgcaaaagc cgtatatgta gcttatcgtc aagcgaacat ggtcgattac
    10021 atttcattcg atgaatttat agataaatgg gattttgata tggaggttgc agtagctgta
    10081 tacagtacta tgatgttcaa acaagcacgt gatgcctatc aaaaagaatt cgaaaaagca
    10141 aataaggaaa aaaagcttca aaagtaaaaa tgccaaagct cttagttgaa acgtgggtcg
    10201 atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt ttaagcgata
    10261 tctgtttaga tgacattttg caaatggctt acaacaagag cgcttatgaa ggatggaaga
    10321 actatgcaat aaatcaatcc cagaaaaact aaaggaaagg aggtaaaaaa tggctaaaaa
    10381 gagaacggaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    10441 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    10501 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    10561 gtatgaatta caaagtcaaa aagttgaagt aactagtcaa cgtttagcca atgccaaaaa
    10621 atattatgga gaaaattcca ccgaagttca gaaacttgaa agagaactga ttaatcaaca
    10681 aacagctcag caaaggttat ctaatgatat agataaaacg agtaaggcat tggctcaatc
    10741 taaaggtgaa tttaaaacgt atgagtcaac tatgaaagac ttagataatg agcaacaaca
    10801 tctaaagtca agcgcatcat tagtagaatc cgaatataaa aaatggcaag caactgctgg
    10861 ccagtctgct acagaatctg aaaaattagc aaaagcacaa gaatatgtcg gcaaacagag
    10921 tgatattgct gaacaaaaaa tcgaagtgct aaaaaaacaa ttagatgcca cacaaaaaga
    10981 atttggagaa acatctaccg aagctttaga aatgaaaact aagcttaatg atgcggaaag
    11041 agaattcgaa gaattaagta atgctgctaa aaacgttgat acctccacag tagatgatat
    11101 cggcaaaaaa cttgatatgg gtaatttaat ggaagctact gatcacttgt cagtgatcgg
    11161 agataagctt attgatgtag gtagtaagtc tattgaagca gctggaaaag cacaagctat
    11221 gcaggcccaa tttaaacaag tctttggctc tttagaaggg gaagcacagg acgccgttga
    11281 gggaatggct gaagaatttg gaatgttacc aaatacgatc aagcctgttt ttatacaata
    11341 tacgtcaatg tttaaaggac ttggatatga taccaaagaa gctatggagt tagctggtga
    11401 tagtactcag ttagcagcag atgcagcagc tttttacgat aagtctatgg atgatgctag
    11461 tgaatctctt aattcattta taaaagggaa ctacgaaggt ggggagcaaa taggtttatt
    11521 tgctaatgat actcaaatgg cagcttatgc tgttaagcat aatttgatac cagcgactga
    11581 aggagcaaaa aaagccagtg aagagtcatt gttagctgtt gaaaaagcac aatctaagta
    11641 tgctgatgct attaagaaac atggtgaagg atctttagaa gcaagagagg ctgctttaaa
    11701 acttaaagat gcgcaagata aaataaatga agaattaggc ccacaaacgc aaaaatggtc
    11761 tgatttagat gaggctacca agcaagcagt tcgagttcaa tatgctgaag atatgcaaaa
    11821 attagccggt gctacaggtc aggctagtag ggagtctgat ggtttagaaa accaaatgac
    11881 aagagcaaag caagcgttag aagattttta tgcctcattg ggtgaagata tactgcctgt
    11941 gtttatcaaa gggttgcaag caggagcgaa agctttgcaa ggattagccg aatggtggag
    12001 taaacttgat gggccaatga aaaatttcat tttagctctt ggaggaattc tagcattatt
    12061 aagcacatta gctcctgtta taaccgcagt tgttacgata gttggcacat ttggttctac
    12121 agttttgctg ccaataatag gaattatagc gggcgttgca gctgtaatag cgattgtaat
    12181 tacagcgttt caaaactggg gcgcaatcac tgactggttt agtgatttat ggaaaaaatt
    12241 taccgattgg ttaggtgata cctgggaaag tataaaagaa agtgcctcat cagtttggga
    12301 tggagtcaaa gaaacctggt ctggatttgt agattgggtt caagaaattt ggcaaggagt
    12361 ttctgattgg tttggagagt tatggagcgg attagttgaa ggagcttcca acatctggca
    12421 aggagtccaa gagacttggc aagcattcgt tgattgggtt tcaaatattt ggaacggagt
    12481 caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa taccatggac
    12541 attaataacg tcattgattc aagctggtat taatattatc gtgggtatct ttgatgtagc
    12601 tggacagtta ttaggcgcag cttggcaagc tgtttggaca cctatttctg atttccttaa
    12661 aaatacttgg gatactatga cacaatggat aagcatcgct tggaatggaa ttgtaactac
    12721 attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga catctattag
    12781 tactacggct tcaaatattt ggaattcaat tagtgcaaca gcttctagta tttggaacag
    12841 tatcaagaat acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg
    12901 gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta cagcatcaaa
    12961 tatctggaat agtgtgacta gtagtataag caatgctata aacgcagcta aaagtgccat
    13021 tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga atttggaacg gtatcaaaaa
    13081 tactgctttg aatctttgga atggaattac aagcactatt agctctaaag taaacgatgg
    13141 aaaaaatgca atttcaagcg gttggtccaa tctaacaggt attgtttccg acatattcaa
    13201 taatgttaaa agtacaattg ctaacatatg ggaaggcatc aaaaagactg ttagcgctcc
    13261 aattgattgg attagagaca aaatcagtgg catttttgat aatttgaata tttctatacc
    13321 acatattccg ttaccggaat ttatcatgga aggcagcttt aacccgctaa aaggtcaaat
    13381 cccccatttg cgtgtaaaat ggcatgctaa aggaggtatc tttactaaac caactttact
    13441 aggtggaatg aacggtgtcg gtgaagcagg acctgaagca gttttacctt tgaaaagatc
    13501 tgttttgcaa gaaattggtg atcgtatctt gagtagcaca tcagtttcat ctagggcaca
    13561 aacgattcaa cctgtgaata actacgaatt caatttcaca attgatggta acgcagatga
    13621 ggttactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaaa ttcaaaatga
    13681 taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta gtattaaaaa
    13741 ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc tgattatttg
    13801 cataaattgg ctttagctga aagaccaacc ataccttctc caaaaagaca gagagttgaa
    13861 acttctgttt taggtaggtt aggtggctta gtacaagatt actcgtttga agatatgtcg
    13921 tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt caagcaatcg
    13981 ttttatatca tgcgtcattg gttaaactat gcaaagaaat tagaattctc tgacgatccc
    14041 aacgtctatt acgttatcca gactatcgat attggggatg cagaaaacga tattgttgaa
    14101 tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca agaagatgta
    14161 cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt agaagaaagt
    14221 tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt aaatgattat
    14281 gtgtttagtt tcgaaggctt agtagcagga actgatgtag tcattgatag tgatttgatg
    14341 ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa aatgaagacc
    14401 atgcaatatc caacgttgca agtggatatt aatcatttta attgtactgg tttgagcaaa
    14461 atacaaattt atcgtaacgg gttaaggtag gtgaaataga tgatcgataa tttaataact
    14521 atttacgata aaaatgacgc gaataattta gctgaacatt tatatgatac gcaaggttta
    14581 ggcgctttgt cagactggtt aacagctact gttagcaata aactaaacgg agccgagata
    14641 tttcagggta cttattcaat aaacggaact aatgcagatt tgattgtaga aggacgtatt
    14701 attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta ttatgcaaaa
    14761 acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa tgatataaga
    14821 aaatcggtgt tgaataaata tgacagcgga acagaaaaga tcactgctac tcaggcatgg
    14881 caaaacgcaa aagttttagc gaaaccagct atcccttcgc agttttcttt ctcgtcatta
    14941 gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt ctttggtgga
    15001 aaagagggat ctattctaga tcgatttcat ggtgagtttc taaaagataa taacacatta
    15061 cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag cgatttatac taaaaactta
    15121 actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta tccattcatt
    15181 agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat tttcacggat
    15241 tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga caaagcgact
    15301 gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat agataaacaa
    15361 aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag aggctatgaa
    15421 aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta tcatccacag
    15481 ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct aaccaattca
    15541 tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac agagaataat
    15601 gtcagcaatt tgattaatga tgccattgat caattgaaaa atggtggcga aatcagtgat
    15661 ttaatcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa tggtggttac
    15721 gttttattag atcctaaaga agcccctagt cgtattttga ttatggacac accagataag
    15781 aatactgcac ggaacgtttt gcaaatcaac aacgctggta ttggtttctc taaaactggc
    15841 attaatggaa cgtatgacac cgcatggacg ttagatggcg gattcaatgc ctcgtttatt
    15901 acagctggtg agatagtagg gattactatt agaggtacta cattaattag tgatggcact
    15961 gattatagaa caagtatagc taatggcaaa atgacttggt actcaaaaaa agttaacaaa
    16021 gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggcat tgtatcatac
    16081 accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt ggtttttagt
    16141 acgtgggata atggtaataa cagaccgttt ttatcttttg gtgcgccaaa tttcagatac
    16201 agtaatgcta gttatataaa tgatggtgac ggtaattctt taggtattga tggtagtgca
    16261 ggtaattcat gggaatttaa agtagctggt aggactatga aatttactag tgacggtatg
    16321 ctaacgttgc caggttgttt ttttggttca tgggaagatg ggaaacttgc tagatttgaa
    16381 caatcaacgg tacaagtata taaagatttt actgttagag gtactaaaaa ctcaaccgta
    16441 ccaacagaac attatggaca acgactattg aacgcttatg aaactccaga atattatttc
    16501 gctgattatg gggaagccgt tacaggtgat gatggtaaag ttcgtgttga tattgacccc
    16561 atgtttgctg agacagtaaa tctaagtcgg tatatgacac atgtgacacc tacagaacta
    16621 gttttgtgtg ctgttactca tgaagatgtt gaccatttca tcattgaaac tagtaagcca
    16681 aacgtattgg ttagatggaa tttagtggca catcgactag ggtatgaaga tgttagatta
    16741 aaagaggata cagcatatga tagcacagtg cttgaccaaa aacgttttta aaacgaagac
    16801 aaggaggtat ataaatggct agcagtttat ataatttggc tttagatttc agcaaagaat
    16861 taaactacac caaagctatt atggctcgtc aaggtgataa agggatcacg gtgactgtta
    16921 aaccgttttt aaatggcttg cagatggata cgagcggcgg aacatttact ttaaaaggaa
    16981 ctacgccatc taaccgttac gtagatagtg tcgctactag tgtaactagt gaagaagtca
    17041 cattttctct tgatggcaca tttatgagtg aagcagggta ttataaacac tgctatgtag
    17101 aatatagaaa agacgatcaa attttaacga cgcaagatat catttttttc tcactaggag
    17161 tgtctgacat ttcgcaaggc caagccgatg aatatgtttc gcaattagaa gaattgattc
    17221 gaaagtataa cgaaactttt gatgcttttc tggctgaaat taaaggtaga atggatagct
    17281 taaataaaca gattactgat ttaactggtc aagctaaaac actacaagac aagttagatg
    17341 ctctgaaaga agaaatttct aaattaggta acttacatgt gatgtactcc aacagcatcg
    17401 acttcggggg ctatgattat agtgggaatc cgaatttaat gtccaaacta aaatcgagcg
    17461 attttaacgt tggttaccac gggtcactaa ctaatgttaa cggaatgtta cattttatgt
    17521 ctgatggtac aggggacatt gatatgttta cgcgtattaa tacacctcag cttgctagtg
    17581 ggaaaaccta tactctgagt gcgaaagttc gatttgatga aggaactaca ggagctattg
    17641 ataaattacg tttggtgtat cgtacatcac caggagaaaa gatattattg gaagcaaata
    17701 atacaactat tacaatagat gatgtaggga aagaaataac aatcaaaggc acagctaacg
    17761 ttaattatca aatcacaaat ttagatcgtt tttatattag tgttagtttt tctaacaggg
    17821 ataaaataaa tggtggattt aaattgtacg acatcaaaat cgaagaaggc tcaacagcca
    17881 ctccgtatca gccaaactta ctagatgacc cttactgggt aggtaaaaca tcgttgggta
    17941 agaatattgc aaataaaggt acgacgttcc caattaaatc tagcgcgtac gaaatatata
    18001 aaggtaacac ggaagaagag cttataatag gtcaaacgta tactatcacg cttaaaggaa
    18061 caaaacccgc aagtcaaacc tttgtagcat ataattattg gaacgttaat tttggagatc
    18121 taaaaccagt tgagggattg acagacgtat ggtctctaac attcacacca acgaaacttg
    18181 aaccgggttt gcctaaagac cttcgcattt ttcagtcgcc taaagaaacg gccggcgcat
    18241 gccgaattga ctggctcaag atcgaaaaag gcgacacacg aaccccgaat attgagcaat
    18301 ataaataccg aggaatcggc atgcgagact caaacaatcc aaaagattat gtatgggatc
    18361 tagaacctag ctatgttgag aataacctag ctacagatat caaggttgct gaaatcacag
    18421 gtaaagcaaa caattatacc gatgggaaag tatcggagat taattcgcag ttgactgctt
    18481 caattaatga agtagacacc acagctaagg atgctcaaac aaaagcgaat gctaatgcga
    18541 ctgctataga tgaattagac aataagatcg atgaacgcat taatgataca gctactacca
    18601 cattaacagt tacaaacggg aataccggat cagcaaagct ttatcgtgaa ggaaaaacag
    18661 tttctatata ttttgtggct ttaaacggaa aaagcagtgg tggaaatgat tcaacgatac
    18721 taacaattcc agaaggctat cggccaccaa ttagttttga gcaactggtt ggctcgatag
    18781 accgttctac tttgaacagt gctcaactat ctattggcgc agacggagcc attaaatgga
    18841 gaagaaattc aagttatgga tcagcttatt catttgttat cacttattca atttaaggga
    18901 ggaaatctaa tgaaagtagt ttacaaatca atcaagcctt acggattcga gcaaatcatt
    18961 ttgaacaatc aagaaaatat ccctgaaaac tgtacagaga ttaaaccacc agttcctaac
    19021 tggagaccaa gatttgattt tgataaaaaa cagtgggttg agttagctac tgaagaagag
    19081 aagagtggca cagcggttga cgatattgaa gatgtcgatc agttgaagca attaaatgct
    19141 ctactgacaa aacaattggc gatatcggta caggaacaag aaaaaatgca acaaatgtta
    19201 gctcaattga cgatggaagt cgcaagtatt aagaatggag ggaaaagtaa tgaatagttt
    19261 tccgggcttt gaaaatataa aacaatttta tgattgggga tgttatacgg accaagattt
    19321 acttgactat gtaaacatga actgtttaac aaaagaccaa tataagcaga ttactggaaa
    19381 tgaaatttaa ttaagcacaa agttaaataa aaagcgtact caaacgagtg cgcttttttg
    19441 cataaaggag aaatagatat tggaaaaata tttaaataca ttctcagtaa tgacaggggt
    19501 gataggcggg acaattgttg gtttattggg agggatggac aatatactac atgttttaat
    19561 ttttcttgtg ggtgtggact tcttaactgg acttgctaag gcatggaaat tgaaagaaat
    19621 aagtagtgag ataggtttcg aaggattgtt gaagaaagtc ttaatcttcg ttgtgattgc
    19681 cgttgcggtt gaagtacaga aaatcgttgg gaattctata cctctaagag aaatagttat
    19741 tatgttctat gttgcaaatg aagggattag ttttttagaa aacatttcgg tattccttcc
    19801 tttgccagat aagttaaaag aggtattcca acaaatacga aatgatactg aaaataaaga
    19861 ccgaggagga accaaatgaa aaagaaaatc tttgtaggag ctattatagc tcttttttta
    19921 ttgccaataa acgcctttgc ttacacgatc aacaatgaat tcaatttggg cgcaaacgaa
    19981 ggtagttctc aagtagcaaa taaccaatac atcctattgc atgaaacagc aaacgaaact
    20041 gcgacaggac gaaatgaagc gcagtacatg aaacgttctt ggtacaatgc ctatacagcg
    20101 tatattgttg gcgatggtgg gatcgtttac caagttggac aacctggtta tgtacaatat
    20161 ggcgctggtt catatgctaa tgcaaatagt cctgtgcaaa tcgaactgca acatacgcac
    20221 gataaagtaa cgtttgaaaa gaactacaag gcatacgttg aattggctag agattcagca
    20281 atgaaatatg gtattccatt aacattagaa acgccttata accaaccagg aatcaaatcg
    20341 catttatggg taacacaaaa tatctggggc gatcatacag atccttacgg ttatctttct
    20401 gaaatgggcg taagtaaaga aaaattagca tatgatttag ctcatggatt taccgatgaa
    20461 aatccgacaa cttcagatga taaaccagtc attgatccaa ctagagcagg tgcagcaaat
    20521 cctacactga cagatggaac aaattacgcc cacattgatc agtttggaga aatcgaaaat
    20581 gcgaacttac acgtcgctgg atggcacatc gctaactata aatacgagta tattttcatt
    20641 atggactaca atactgggaa agaattagct cgagtaagag ctgatggaat ttatagacca
    20701 gatgtaaacc aagcttataa tacttctgga aatgttggtt atcatgtatc tttcaatatg
    20761 cgtaattttc ctagtaagaa agtctatgta atgatgcggg caacgaatga tccagaggga
    20821 aacactaaag gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa
    20881 cgataaaaat agctcctcgt tgaggagcag tacataacta tattgacaac tataaaaatt
    20941 attcgataaa atagtgatgt tatcgcatat cttcactatc acccataaat agtcacactc
    21001 caagctatgc gataacaggt ttgttgccac acattctact ggttgattgt ttatggcttt
    21061 atgtggcaac aaccagtacc cttagctcag ttggtcagag cagacggctc ataaccgtcc
    21121 ggtcgtaggt tcgagtccta cagggtacat taacgtagcc atttgaatcg ttctgtgtta
    21181 gaattttttt gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt
    21241 ggctttttta tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatacttct
    21301 gtatatttga aaagttttac tttgattttt aaatagaaag acatttgggt taaattgtga
    21361 gataataata aagaagagtt taaagcgcac cccaaaccac ttccccataa gtgtgttacg
    21421 ctttaaactc ttttatattt gaagccatta aaaagcatac catatttttg aaaaaaagtg
    21481 agaaaaaagg cttacaattg gagtggtagt taattagtga cttatttttg attttatagc
    21541 actgatacta taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa
    21601 tatctccttt cacaagtatg gtgataaaat tcgttccggg ctaccttttt aggtagccta
    21661 ctttaatctt tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga
    21721 tgatcgctac gtgcgtccgg cacgtcagtc acgatatcaa acaaaaagta tacacccttc
    21781 ttcattctag ttttcgcagc aggaattttg aaatagttct tattagaata gtagagattg
    21841 attaataagc tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa
    21901 tctttgacaa atttttctga tggatcgtat ttaaaccaaa gcagatgttc ctctagctcg
    21961 attgtcatgt ttttcacctc aacgaaagta tacgaactaa tgtttgtttt gtaaaggata
    22021 aaaatagggt agcaaccggt acccaaacta gtacccattt atcaaaaacg acgaaattta
    22081 tgtaaataga aaaataagaa taaacattga tgtatcaaca tttatatatc tttagaacaa
    22141 atgaaaaggg tggttctcat aagaaaaaca tgttcgcact tctaggcaaa ccaggatttg
    22201 aagaccttgc taaagaattg aacgaacgcc tgtaaaataa ggatttgtcg ctagatttaa
    22261 aaaatcagtg atagaaacat gtttgcttta aaatgaaact agtgatagtt ttagtgatag
    22321 tgaacttaaa aaaagcactc aatttgagtg cttttttatt gttattaaaa attaaaaaac
    22381 taaaatacat acccgaattt catacccgaa atataaaaaa tgtcatcacg tacatctttt
    22441 tcgaaactac cgaagtagcc aaagatgtac tgatgacgtg ggtttggcac ccaatgggcc
    22501 gtgaggggct cgaacccgcg acccgctgat taagagtcag ctgctctacc aactgagcta
    22561 acggcccgta caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta
    22621 catttaaaga aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc
    22681 acatttgctc gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc
    22741 ctaacacttt tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct
    22801 taacgataaa agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta
    22861 atcaaatcac ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt
    22921 tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg
    22981 gggaaggaac tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt
    23041 tatatggacc aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa
    23101 aagcatccat tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca
    23161 gcaccagtta aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg
    23221 ttgaaacaag tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca
    23281 gtaggaattc gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg
    23341 gtcagccaat tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta
    23401 gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa
    23461 acgattgttc tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat
    23521 cgcaatttta gtagattcag gtacggatgt gccagaagaa ttgataaaaa aatacgagat
    23581 gtatatgatc ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat
    23641 tacctctgaa gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga
    23701 tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact
    23761 tgcggtaaca atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca
    23821 acagcaagaa gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg
    23881 tttttctgcg atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat
    23941 cagtaatctg aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga
    24001 atatcttcaa aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa
    24061 gcttaaccca atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag
    24121 gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc
    24181 aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga
    24241 agcgagactt aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc
    24301 ggcattagta gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg
    24361 ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt
    24421 agaacaaaaa ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag
    24481 gcaaagatga aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa
    24541 gaggtggctc tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat
    24601 cgtcaaattc aatttagcca aagaaggcta tgatgtttat actgcatatg atggggaaga
    24661 agccttggaa aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc
    24721 taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat
    24781 tatggtaaca gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga
    24841 tgattacgtg accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct
    24901 tcgtcgcggt gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat
    24961 tggtgatttg acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga
    25021 gttaacacat cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat
    25081 gacaagagaa catttattac aaacggtttg gggttatgat tattttggag atgtacgaac
    25141 agtggatgta actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa
    25201 ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt
    25261 ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta
    25321 ttctgatttt attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac
    25381 gttccacgat caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa
    25441 caatcagccc ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata
    25501 tcaaaagatt tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg
    25561 aaaaagggat catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa
    25621 atgattatgt tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg
    25681 ataaacgggt gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg
    25741 gggctcttta cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag
    25801 tcattttctt tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag
    25861 ttgctcgttc gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta
    25921 gaggagacta cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg
    25981 ataccttcaa tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac
    26041 gcaatcgttt ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca
    26101 ggggtaaagt cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg
    26161 aagcaatcgg acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga
    26221 aattattaga agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg
    26281 atcagatgat cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg
    26341 gattagtagc tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg
    26401 aattcgtctc taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata
    26461 tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg
    26521 taacgctaga tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc
    26581 gcatggatac aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga
    26641 attttgttct tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc
    26701 gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag
    26761 tagtcgataa tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt
    26821 gtcgcttatt agaaacgcat aataacgtgg ttttaagtat caccgatcaa ggattgggga
    26881 ttcctaaaaa agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg
    26941 caagagcaca aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc
    27001 atcgaggcgc tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc
    27061 tgccatatga accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg
    27121 tataggactc attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag
    27181 cccagcagga aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca
    27241 aagcaatcga aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg
    27301 aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa
    27361 agcaagattt ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt
    27421 gaaaaatctg aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata
    27481 caaggaagca tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat
    27541 gtattttact agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc
    27601 gaaccatctc gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt
    27661 aacagcaaaa gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca
    27721 atatgctacg aaacattcga tcaaactgaa aaagtatagt tatatcctgt cacagcaacc
    27781 ctatacactt tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga
    27841 aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca
    27901 agtagttgat tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga
    27961 aagtttcccc tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg
    28021 cagtgacaat acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga
    28081 cagcagagga cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa
    28141 tatccaaacc agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact
    28201 tgcaagtacg aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat
    28261 tggttcagtg atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga
    28321 tctcttacct gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct
    28381 ggaaaaatta cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt
    28441 tttcgtcgct tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata
    28501 ccaagaaaac aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag
    28561 cgacgatatc acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag
    28621 cgcggagcag accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg
    28681 cttgctggca agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc
    28741 agatgctgaa aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt
    28801 atctaacaaa gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca
    28861 tttagaagaa gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa
    28921 tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac
    28981 gaaatacatg cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta
    29041 tacggaagag gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa
    29101 aattttatgg cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta
    29161 tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagtgt
    29221 caacgctttc agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata
    29281 aaaaagcgag tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc
    29341 tgaagaaaaa ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact
    29401 ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat
    29461 cacttccttg atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac
    29521 gcatgaacat cgagatcata tacatggcgt gggtgtcttg gctcgtaagt atcatttgga
    29581 tgtttttgcc aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt
    29641 ggaacaaaaa catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga
    29701 gagtttcggc gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa
    29761 tcggtcattc gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat
    29821 tgaaaatgcc gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg
    29881 accttatccg tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga
    29941 agacggtgca cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg
    30001 ccatcttagc aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt
    30061 gaaagaaaaa gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga
    30121 agcaactgag ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga
    30181 taaaatcaat cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat
    30241 aaccgtatta tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct
    30301 catctactaa tgcaatcgcg taatctgcca tgctgatcgt actgtcgcca gcagcgtttc
    30361 tttgtaaata atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata
    30421 aagaagctgg actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc
    30481 cttcgtacat attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca
    30541 tacgagtcgt cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc
    30601 gtgtatcggt tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt
    30661 cttttccttt gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag
    30721 ttgtcaactc aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat
    30781 gacgcacgat tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac
    30841 ctgcatggcc agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct
    30901 gtccattgta agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg
    30961 cgcattcctt tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata
    31021 aaaatagaag ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa
    31081 tgataacatc tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca
    31141 cgatcccaat aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt
    31201 acatcgctca gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg
    31261 ctacatttga tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag
    31321 aagaagacat cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta
    31381 ttcagccact aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag
    31441 cagttacaac tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag
    31501 ggtggaagca aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc
    31561 caatcattgt tatagtcact tttacgctta tcggttcagc ggtttttcaa cagttgttca
    31621 gaatgactgt tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag
    31681 acacgttgtt tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc
    31741 ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag
    31801 cgaatgcaga aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg
    31861 ttactcagca atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg
    31921 ctgtattttt tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg
    31981 ctcttttacc agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga
    32041 tgaacccgat gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct
    32101 attccgcttt atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa
    32161 caccgccgat cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta
    32221 tcgtgttgtc aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa
    32281 actgcaaaaa ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa
    32341 aaacaaaaat actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt
    32401 atttatagtc atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat
    32461 gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct
    32521 aggctgaaga agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac
    32581 gcaaacagca aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc
    32641 cccagtatac tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc
    32701 aaaataaaag cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg
    32761 gttgctttgc caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg
    32821 acaaagattc caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct
    32881 gattcgatcg ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct
    32941 agtatataca tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc
    33001 gaagctagga tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta
    33061 tattttttta aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct
    33121 tatatcgatt gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc
    33181 ttttctttga tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg
    33241 ttagcactcg aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat
    33301 cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac
    33361 tgtcggagga atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt
    33421 tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc
    33481 aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga
    33541 atatctgatc gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa
    33601 aaaataatca aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca
    33661 gaagacgcac gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta
    33721 acattaggac ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc
    33781 acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg
    33841 ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca
    33901 acgacagcaa ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca
    33961 ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa
    34021 gaattgcata atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct
    34081 gtttcttcgg gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga
    34141 aacgacggtg tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta
    34201 gaaggtatgc agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa
    34261 atggaagctg ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc
    34321 caagatattc tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt
    34381 gctgacgatg tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca
    34441 ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa
    34501 gatatcgcta ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa
    34561 gatgtaacaa tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca
    34621 acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa
    34681 aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct
    34741 aaattagcag gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa
    34801 gaattaaaat tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga
    34861 atggtctcag gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa
    34921 gcagaaggag acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt
    34981 cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac
    35041 gtggaactag gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca
    35101 ggtatcgtag acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct
    35161 gccttgttat taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca
    35221 gctcctgcaa tggatccatc aatgatgggc ggtatgatgt aagaaaaagc aacttgctta
    35281 tctacaactg atcattagtg aaagacagat aaaaagaggc tggaacagaa gcgtttaact
    35341 ccaagaaata agaagaaatt cacggaaatt gcttttcaaa tttttgtgaa tttcagctta
    35401 tttccgaagg agttgcttct gcttccgctg tttatacgtg tttagagcgt gagacaaaag
    35461 tgttctttac ttttgtccca cgctcttttt tgtgtgtaat tataatgaaa atagttattt
    35521 ttaatagtta taatatttta tctattaaaa gtaaatatcc att
//
LOCUS       DAZKAY010000019        35533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000019 DAZKAY010000000
VERSION     DAZKAY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.019"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACG8WP_000855"
     CDS             complement(1..120)
                     /locus_tag="ACG8WP_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9027181.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACG8WP_000856"
     CDS             299..1960
                     /locus_tag="ACG8WP_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9027182.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ACG8WP_000857"
     CDS             complement(2030..2377)
                     /locus_tag="ACG8WP_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9027183.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG8WP_000858"
     CDS             2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG8WP_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9027184.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ACG8WP_000859"
     CDS             3403..4446
                     /locus_tag="ACG8WP_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027185.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ACG8WP_000860"
     CDS             4452..5522
                     /locus_tag="ACG8WP_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027186.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ACG8WP_000861"
     CDS             5512..6465
                     /locus_tag="ACG8WP_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027187.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ACG8WP_000862"
     CDS             6477..7487
                     /locus_tag="ACG8WP_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9027188.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ACG8WP_000863"
     CDS             complement(7772..8593)
                     /locus_tag="ACG8WP_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9027189.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ACG8WP_000864"
     CDS             complement(8690..8968)
                     /locus_tag="ACG8WP_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027190.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ACG8WP_000865"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ACG8WP_000865"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9027191.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ACG8WP_000866"
     CDS             complement(11724..12311)
                     /locus_tag="ACG8WP_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027192.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ACG8WP_000867"
     CDS             12486..13808
                     /locus_tag="ACG8WP_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9027193.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ACG8WP_000868"
     CDS             13963..14739
                     /locus_tag="ACG8WP_000868"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9027194.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG8WP_000869"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG8WP_000869"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9027195.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG8WP_000870"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG8WP_000870"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9027196.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ACG8WP_000871"
     CDS             16629..17258
                     /locus_tag="ACG8WP_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027197.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /locus_tag="ACG8WP_000872"
     CDS             complement(17382..18491)
                     /locus_tag="ACG8WP_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027198.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG8WP_000873"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG8WP_000873"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9027199.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19309..19407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19445..19981
                     /locus_tag="ACG8WP_000874"
     CDS             19445..19981
                     /locus_tag="ACG8WP_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9027200.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ACG8WP_000875"
     CDS             complement(20021..21784)
                     /locus_tag="ACG8WP_000875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027201.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ACG8WP_000876"
     CDS             complement(21787..23502)
                     /locus_tag="ACG8WP_000876"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027202.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="ACG8WP_000877"
     CDS             complement(24281..24505)
                     /locus_tag="ACG8WP_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027203.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="ACG8WP_000878"
     CDS             24696..27473
                     /locus_tag="ACG8WP_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9027204.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="ACG8WP_000879"
     CDS             27554..27973
                     /locus_tag="ACG8WP_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027205.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="ACG8WP_000880"
     CDS             27985..28455
                     /locus_tag="ACG8WP_000880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9027206.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="ACG8WP_000881"
     CDS             28472..29251
                     /locus_tag="ACG8WP_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9027207.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="ACG8WP_000882"
     CDS             29238..30059
                     /locus_tag="ACG8WP_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9027208.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="ACG8WP_000883"
     CDS             30076..31125
                     /locus_tag="ACG8WP_000883"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9027209.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="ACG8WP_000884"
     CDS             31138..32142
                     /locus_tag="ACG8WP_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9027210.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="ACG8WP_000885"
     CDS             32775..33794
                     /locus_tag="ACG8WP_000885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9027211.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="ACG8WP_000886"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="ACG8WP_000886"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9027212.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="ACG8WP_000887"
     CDS             35018..35395
                     /locus_tag="ACG8WP_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027213.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT        11643 a         6242 c         7292 g        10356 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gccacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gat
//
LOCUS       DAZKAY010000020        34869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000020 DAZKAY010000000
VERSION     DAZKAY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.020"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8WP_000888"
     CDS             complement(<1..210)
                     /locus_tag="ACG8WP_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFP9027214.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSW"
     gene            complement(203..1165)
                     /locus_tag="ACG8WP_000889"
     CDS             complement(203..1165)
                     /locus_tag="ACG8WP_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9027215.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            complement(1198..2283)
                     /locus_tag="ACG8WP_000890"
     CDS             complement(1198..2283)
                     /locus_tag="ACG8WP_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9027216.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLDLYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            complement(2302..3342)
                     /locus_tag="ACG8WP_000891"
     CDS             complement(2302..3342)
                     /locus_tag="ACG8WP_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9027217.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(3358..4749)
                     /locus_tag="ACG8WP_000892"
     CDS             complement(3358..4749)
                     /locus_tag="ACG8WP_000892"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9027218.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            5127..6110
                     /locus_tag="ACG8WP_000893"
     CDS             5127..6110
                     /locus_tag="ACG8WP_000893"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9027219.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(6478..8616)
                     /locus_tag="ACG8WP_000894"
     CDS             complement(6478..8616)
                     /locus_tag="ACG8WP_000894"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9027220.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(8655..10241)
                     /locus_tag="ACG8WP_000895"
     CDS             complement(8655..10241)
                     /locus_tag="ACG8WP_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9027221.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(10231..11451)
                     /locus_tag="ACG8WP_000896"
     CDS             complement(10231..11451)
                     /locus_tag="ACG8WP_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027222.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(11463..12269)
                     /locus_tag="ACG8WP_000897"
     CDS             complement(11463..12269)
                     /locus_tag="ACG8WP_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027223.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(12479..13759)
                     /locus_tag="ACG8WP_000898"
     CDS             complement(12479..13759)
                     /locus_tag="ACG8WP_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9027224.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(13803..15836)
                     /locus_tag="ACG8WP_000899"
     CDS             complement(13803..15836)
                     /locus_tag="ACG8WP_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027225.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(15870..16721)
                     /gene="rfbD"
                     /locus_tag="ACG8WP_000900"
     CDS             complement(15870..16721)
                     /gene="rfbD"
                     /locus_tag="ACG8WP_000900"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9027226.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(16819..17847)
                     /gene="rfbB"
                     /locus_tag="ACG8WP_000901"
     CDS             complement(16819..17847)
                     /gene="rfbB"
                     /locus_tag="ACG8WP_000901"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9027227.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(17869..18441)
                     /gene="rfbC"
                     /locus_tag="ACG8WP_000902"
     CDS             complement(17869..18441)
                     /gene="rfbC"
                     /locus_tag="ACG8WP_000902"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9027228.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(18455..19321)
                     /gene="rfbA"
                     /locus_tag="ACG8WP_000903"
     CDS             complement(18455..19321)
                     /gene="rfbA"
                     /locus_tag="ACG8WP_000903"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9027229.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(19421..20128)
                     /locus_tag="ACG8WP_000904"
     CDS             complement(19421..20128)
                     /locus_tag="ACG8WP_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9027230.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(20133..20960)
                     /locus_tag="ACG8WP_000905"
     CDS             complement(20133..20960)
                     /locus_tag="ACG8WP_000905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9027231.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(20960..21760)
                     /locus_tag="ACG8WP_000906"
     CDS             complement(20960..21760)
                     /locus_tag="ACG8WP_000906"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9027232.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(21753..22892)
                     /locus_tag="ACG8WP_000907"
     CDS             complement(21753..22892)
                     /locus_tag="ACG8WP_000907"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9027233.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(23097..24290)
                     /locus_tag="ACG8WP_000908"
     CDS             complement(23097..24290)
                     /locus_tag="ACG8WP_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9027234.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(24369..24575)
                     /locus_tag="ACG8WP_000909"
     CDS             complement(24369..24575)
                     /locus_tag="ACG8WP_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9027235.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(24568..25710)
                     /locus_tag="ACG8WP_000910"
     CDS             complement(24568..25710)
                     /locus_tag="ACG8WP_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9027236.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            26222..26857
                     /locus_tag="ACG8WP_000911"
     CDS             26222..26857
                     /locus_tag="ACG8WP_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9027237.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            26993..27085
                     /locus_tag="ACG8WP_000912"
                     /pseudo
     CDS             26993..27085
                     /locus_tag="ACG8WP_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            27199..27834
                     /locus_tag="ACG8WP_000913"
     CDS             27199..27834
                     /locus_tag="ACG8WP_000913"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027238.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            27941..29179
                     /gene="mef(H)"
                     /locus_tag="ACG8WP_000914"
                     /gene_synonym="mefH"
     CDS             27941..29179
                     /gene="mef(H)"
                     /locus_tag="ACG8WP_000914"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFP9027239.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            29406..29825
                     /locus_tag="ACG8WP_000915"
     CDS             29406..29825
                     /locus_tag="ACG8WP_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9027240.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            29830..30069
                     /locus_tag="ACG8WP_000916"
     CDS             29830..30069
                     /locus_tag="ACG8WP_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027241.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(30294..31217)
                     /locus_tag="ACG8WP_000917"
     CDS             complement(30294..31217)
                     /locus_tag="ACG8WP_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9027242.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(31249..32013)
                     /gene="map"
                     /locus_tag="ACG8WP_000918"
     CDS             complement(31249..32013)
                     /gene="map"
                     /locus_tag="ACG8WP_000918"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9027243.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            32171..32611
                     /locus_tag="ACG8WP_000919"
     CDS             32171..32611
                     /locus_tag="ACG8WP_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9027244.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(32789..33358)
                     /locus_tag="ACG8WP_000920"
     CDS             complement(32789..33358)
                     /locus_tag="ACG8WP_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027245.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            33612..34844
                     /locus_tag="ACG8WP_000921"
     CDS             33612..34844
                     /locus_tag="ACG8WP_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9027246.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT        10758 a         6836 c         5921 g        11354 t
ORIGIN      
        1 ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca aaactataat
       61 tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag tactaggcat
      121 aaaataaagc agacaaatga ttaaatttat tactcgaaac gcatctcgac ttaataaact
      181 cttctctaaa acaggtacta ttctattcat ctatatttcc aactttcaaa atttctttat
      241 caattctatt taatagttta tcagagtaat ccgatttaac ttttcttaca aataattttt
      301 cactttcttt tatttgatct atatcccttt catcaaacca tttgttcatg gaaggatcaa
      361 taatatgaat atttgctaat ttcgaagttt gtaaagtagt ttcttcaata tttttcaatt
      421 caagtggtaa atctggtcct ccaataacat ttttattttt gaacgaggaa ttaaaaaaaa
      481 gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc aggtagtctt
      541 gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac aaagcccacc
      601 attgagaacc atgatacaaa tcaaatttat ggtgtctaat ttttggggga agtttaatca
      661 tcctcagaca acgattaatt atagctctaa aaattttaaa tgcagttgtg ttattcctat
      721 taattctagg ataatcatgt ttttgatata catctatatg tttataataa aattctttat
      781 gtttaatttg atctagatct attccacgaa taaactccac tgaactattc tttttaaaat
      841 aattataaat atactcatta tctttgattg gataatcagc accagataat aatacatatt
      901 ttaaatattt ctcattttgt aatgctctct ttactaaatt gatttctgct tgtacaatgg
      961 aaaaaccacc ccaataaatt ttaacacggt ttttattaca taaaaattca acattatcat
     1021 atgatttaaa tgcttcaata aatggttcta tatcagtttt tttatctata tgaataataa
     1081 aaactgcatt ttcgttcaat cgattgacca atctctttaa gttgccaata tcattgtatg
     1141 ccaatatcaa ataacatatt ttcaaaaata atcactccta aaatatcttg aacattttta
     1201 atgatctcta ctatatatac tctccatttg ttcagtagtg tacgaaacat cataactctt
     1261 tagttcttgt gttattttat tagcatacgg ctcttttatt actttttttg cccattcttg
     1321 tggtgtttca tctaatgata caaaatccac attatttgtt aactttgtat ctttggaaat
     1381 agtatctgct aagactaatt ttaattttgc tgcttgtgct tctaatccaa ccattggcag
     1441 accttcataa agagaaggca ttacaaaaac atccatcaca gataataact cgggaatatc
     1501 gtttctttct cctaaaaatt ttactctttt ctctatatcc aattctactg ctttggcttt
     1561 cagtttattt ctaagtgacc cttcacctac taaaatcaag taagaatcag aattcatttt
     1621 tagtatttct aaaaaaatct ctaacaaata agattgattt ttttgtttct ctaatcgtcc
     1681 aatgtttccc acaacaaaag tactcattga gatatccaat tcttttctct tattaacctt
     1741 agcttctgaa tttttactat aaagatctaa atcaatacca ttatgaacta tctcaaaggg
     1801 gtggttttga aacagacttt caccagcttc ttgactaacg gcaaggtagt ctgttgcatt
     1861 ttgtaacaac aatttggtta aaaatttacg caatacattc attacaatat tttgagattt
     1921 tccatcttca gttgcatggc tatgagcaat tctctgtctg attccattct ttttggctaa
     1981 ccataaatta attcccccgc caaaataaac ctgattgtga actatagaaa aagattcttt
     2041 ttttagtaac ttattcactt ctattaaata tttaaaaata tttttcttag gattaggtac
     2101 aacaaaaata cggcctccta atctttcaat ctctgaatga tagtcttgaa catctgcata
     2161 atcttcataa atcaaaaaat caaattgata gtttgtacga tcaattttac gatataaatt
     2221 catcatgaaa gactctgctc cacctaaacc cattcgtccc tgaaaatgca aaattctttt
     2281 cattatattt tcctccatta actaatatac tcgcttcaaa gcagccatta accctggcat
     2341 tttcacttca acgaagcttc caacttttac agcagataaa aatagtggag ctggcatatt
     2401 gtaaagcaac tttgttattt tttttgctga agaatccgtc ttagcaattt gcttggcttc
     2461 tcggatcact ggatcactca atatctgctt tgcttcgtct ttcttctctt ttgaagttct
     2521 tgcagaggta ctataaatag aaaaaataac tgaagttgtc ccttttaaaa gaagcgccgc
     2581 cataccgtct ttattaatat cgtctgttac agtatttgct aatgaataga attctttaaa
     2641 tgtttcgaac ttgttaggtc ggtattgatt agtgatcccc ttcccacctt caaattcata
     2701 aatatattct acttttgggt tcatgatcac ttgatgacag tattcataat aatgtaagtt
     2761 aaatattcga tcctcacaac tattataagt tttaaataga atttggttat ttttcagaat
     2821 atcgctacga tagcatttgt tccatacaac atacatcaac tgttcattca ttaactgata
     2881 catgtctttt ataaactctt cgtgagttag atagtgacat tctgtaacta agttttcttt
     2941 ttcggaaacg attcttccct cgtccattgt acgaaaaaca aacgaactaa caacaagatc
     3001 cgcttttgga ctcttttcta gcaaatgatc ataatctgac aagatcgttt ttactggcgt
     3061 gtcatctgca tcgaagaata agagatattc tccttttgac tgtcttattc cttcgtttct
     3121 tgctaatccg ggcccttgat tggtcgtagt aatcaattca aaacgttcat cattttcaat
     3181 cagttcttga atgatttcct gtgtttgatc tgttgatcca tcatttacta aaacgacttc
     3241 gtactcttca taatccaatt cttttagaat atcaatcgtt cgaccaatag ttgcttctgc
     3301 attgtatact ggtacgatta tgctgaattt cggattgttc atttttacgc ctcccaacta
     3361 gcgaaagatt ttttcattcg gaaaagcaat ttctgccaat gagatcacat tttcgtcttc
     3421 ttcaagtcct tgagaggaca ttttatcaaa taggatcttg atggtttgca gcaagatctt
     3481 taaatcaagc atcaatgaat attttttgat ataaagtaaa tcaaaacgta atttactgtt
     3541 gtaatcagag acatattttc catagacttg tgcataccca gtgataccag cacgaacatt
     3601 atgccgtaag tagtaataag ggttttctgc attgaactga tcgacgaaga aaggacgttc
     3661 aggacgtggt cctacgatcg acatatcgcc tttcagcaca ttgaagattt gtggtaattc
     3721 atcaaagcgg acagctcgaa taaatttacc tacaggcgtt actcgtgcat cattgctctt
     3781 agctaaaact ggtccagatt ttgcttcggc tgtggcggtc atcgagcgga atttatagat
     3841 tgaaaattcc ttttgatttt ttgtgatccg cacttgttta tagatgattg gtcccggtga
     3901 tgtcactttg actaaaattg ctgtgaataa cataaatggt gaagcaataa ctaataaaac
     3961 aagtgaaacg atgatatcaa acaaacgttt gaatagttca tattcaggtt ggattctaaa
     4021 tccagatgct tcaataatac tttcatcttc gaaattcata atatttggat tgatcaatgt
     4081 caagttttca aatgttgtat tcaaaaacaa ttttttttca tttttagtaa ctaattgata
     4141 gattttgatt ttttcagttt cttcgatatg agaagcaaga tagacaatat cgatgtcttt
     4201 aatcatattt ttgatgtttg cataatagtt atcaagaatg actgcttcga ctttatgttt
     4261 gttgtttttc tttgaaccaa aattttggat tgctggtgcg acatcctttt ctaacccaat
     4321 aatgacgacc tttttatctc gacttacgcg taaataaagt ttatatacga gaatacgata
     4381 gatacttaag aaaagcgtac cgagaacaaa tgaaagtaag ataacaagac gtggaaaagc
     4441 aaaccagcgg cccgcaaaag tcactaccat gatccctagt gtcatcaata cctgaccgat
     4501 gactgtgaca aacacgatgt cactgatcat tcgattataa aaaacatatg cacccaacaa
     4561 aatgttcaac gcaataaaaa tcactgaaat aataattgct gagtgttgga aagtttctaa
     4621 atttcgtaat gggatctctc cacgaaactt cagtaagaaa ctactatata ttgctgcatt
     4681 gtatacaata acatccatta atataatgaa tatccgtcgt gcagcactcc attctccatt
     4741 tttattcata tatacactcc taatcacaaa ataatatctc tattatctta ccatagtcac
     4801 ctttaaaaac ctagtttata agcaaattgt aatggaattt aaaaatgtct aataaaaagc
     4861 tttatacacc tctaatcaag acaaggactc taaattacaa atctccagat gaatcgattt
     4921 tcactaatgt tggtaaatta aagaagtcga agtatttgct gcttttttta tccactcata
     4981 aagtgaacct ttatattttt cagagaatcc atcttcccaa agcgctgtct agtccataag
     5041 aatacctgta ttacgtgaag aatcgaaaaa attgaaatcg atttttaatg acattatgct
     5101 tatagaagaa agtgaggtaa tatcttatga ataaacaaga gttgcatgat tttattggtt
     5161 ttcgacaacc aaatatatgt caaattgtcg catataagga tggggtagaa gtttatacag
     5221 atgaatggaa tggttataaa aaagacgata cttgtcatat catgtcagca acgaaaagta
     5281 ttgtatcttt gctgattggc attgctttag agcaaggatt aatcaaaagt atcgatgata
     5341 aagtattaga ttattttcca gagtataaag tgaaacgcgg ggagaaaact atttacgcta
     5401 ttacaatcaa gcacttgcta acaatgaaag caccctacaa atgtaaaggc gatccatgga
     5461 caaaagtctg ttcaagtgaa gattggacaa aaacatcact agatttattg ggaggaagaa
     5521 aaggtataac cggcgaattc aactatcaaa ctgtctgttt acacatatta actggcttgt
     5581 tgtatcagac aagtaagcta acaactgttg attttgccaa taagtatcta tttgatccta
     5641 tagggatacc aaaccatgtc aattattatg ctgaaacagc tgaagaacat aagcagttta
     5701 caatggataa gtctcctaaa accaatgtct ggttttgtga tccactaaat atcgggacgg
     5761 caggatatgg tttgtgttta tcggcaagtg atctggcaaa aattggtgta ttatgcctaa
     5821 ataaagggat gtataaagga aaacaaatcg taccacccat gtggataaac gagatgttca
     5881 aacctagaac cagtgcttta gaagacttac aaaatatgag atatggctat ttatggtggg
     5941 tattagattc tgagaacgaa atatattcag caattggtaa tagtggaaat gttctttata
     6001 ttgacgctaa gaacaatctg gttatctcca tcacttctta ttttaaaccc atgataattg
     6061 atagagtggc ttttgtagag gaatacctta aaccatttat tttaaaatag cctatattaa
     6121 aataaaatat cgattctttg acagctaggg ttcgtgaaac tttggctatg attttgtatc
     6181 tattggcgct ctgagcatga ataagagcgt gggacaaaag taagaaacac ttttgtctca
     6241 ttctttaaaa acgaataaac ggtgggagca gaagcaactt cttcttaaat aagccgaaat
     6301 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttctcgga gttaaacact
     6361 tctgtcccaa cctcgactta tttttttgag tcggcacctt ctgccttttt ttgacaaaaa
     6421 actataaaaa gccgttatgt gcgagcgaat cacaacataa cagcttttta ttatagttta
     6481 atctggtctc aacgaaaagt cgccgattcc ttcttttgtt aaattatcat tgtaaaatgg
     6541 atcatgcgca atatatgctg gccatttatc ttgcattttt ttgatttcac cagcaaaacg
     6601 tctttgtttt tctggagtat cttcataacc tcttgattta gattcgaaat ggtacaattc
     6661 tgcttgatgt gcatagacat tgtaacgacc cagttcataa actttcaagc acaagtcaac
     6721 atcgttgaat gctacttcta atgtttcatc aaacccgtta acagcattga agtcagctgc
     6781 cttgaccatc atacaagctg cagtgactgc taagtaattg acgtcaatga ccaatcggcc
     6841 gaaataacca caatgtgttc gatcatagtt attcaatgca tgccctgcaa caccaccgat
     6901 tcctaataat actcctgcat gctgcgttgt atcatctgga taaaacagtt ttgctcctac
     6961 acagccgatt cgatcaaact gtgcataaga aaccatcacc gtcatccaat ctggctcaat
     7021 cacttctgta tcgttattca agaataaaaa gtatttgccg tttgcttttt ctgctgctaa
     7081 attgttaatt cgagaataat tgaatggaat gtcaatcaat tctacgataa agcggtcttt
     7141 cagctgatgc ttgtattctg caaataattc ttgcattttt ggatctgtac ttccgttatc
     7201 cgcaataatg atttcataat ttgaataaga cgttttttcg atgatcgaat ccacacaagt
     7261 ttttagatct tcatagccat tcttcgttgg aatgatcaca ctgaccaaat cctcttgcaa
     7321 gacgtcataa gcaatctcat aaaatccaga aattctgcca ggacgtacgc ttcctttgat
     7381 gtttcttcta gacagggcat ctgtcaatgc tttcactccg gaatcataga tataactttt
     7441 ggcttcgcca ttacttgcag ttgatcctgg gatcgtccgc caatgataca agactcggtc
     7501 aatgtggtaa atatgatctg cagggatttg ttctgtcaca cgaaggacca aatcataatc
     7561 ttgagaacct tcatagcctt tacggaaacc gccaagttct ttaacaatgc ttgttcgata
     7621 tacgcctaag tgagagatat aattatttcc catcaatgta tcaggcgacc agtctgcttt
     7681 gaaatgcgga tcaaagcgat tgccatctgc atcgatttta tcttcgtcac tgtagatcac
     7741 atccagttct ggatgtacgt ttagcacttt tgccacttca tataatgcaa atggcggtaa
     7801 ttcatcatca ttatctaaga gtgctataaa ttctccttct gctatttcca atgcagaatt
     7861 agttgccaga ctgatgtgtc cattttcttt tcggaaaacg actttgattc tatcatcttt
     7921 tgcttgatag gattccagac actttcggat agctggatca gtagaagcat catcactgat
     7981 acatagttcc caatgatcat acgtttgatc cagtaccgaa tcaatgcatt tttcgagcca
     8041 tttgatttca acgttataga caggcataag tatggaaatc aatggacggt acgcaaatcc
     8101 ttgtgcttct ttcctttgct ctttcaaatc tggtttctca tgacgactga tccactctgc
     8161 atagtcgatc gatcctggct tcaattccaa tttgacccga cggatcgctt tttttacgcc
     8221 atgagtcctt gcataattta tccctttttt cagtaatctt ttttttcttt cccatgaaga
     8281 ttcaatgccg tcatcataag ggtatttacc gtttaatttc acagctatgc cattttgatg
     8341 tctcgctgtt tggaagtcta agatggcttt tcctctcatt ttccctgata aacgtacctt
     8401 aaaaccgctt tgggtcttaa catcaagatt atataaatga ttgatatcca atcttacgac
     8461 atgtgttgct tcagcaatca cattttcttt ttctaccttt atcgtaggga tttctttggt
     8521 cacttcatca attgcccagc ccacaataag caaatcgcca gtggcccgtt cacgagtgat
     8581 ttgatcgata aatacggcaa tttctttttt ttgcacgatt cgtttactcc ttatctttat
     8641 aagttaatca ttatttacat ttcaatgatc catgattggc cattttgagc tactgcttcc
     8701 actaagaatg aacgttcttg ctcgcaaagg attcgtaact caaatcccgc ttctgttcct
     8761 tttgcgagct gttctgcttg attgacttct tctcgtgaaa caggaattgc ttcgaaaaat
     8821 ggcgattggt cagctgataa cttataggac aagggctggc gatccgaatt agcgattccc
     8881 catccgatga tttttaatat ttttgtttct gcatcccaga ttttctcgtc aatacatata
     8941 gaaagttctt tttcttggat ttttttcgaa gtttctttct tacgtcgtcc tagtctatgt
     9001 tttattgtcc attcatcact ttccaacaat gtgcaatagc gatttgtggt tgtttttaac
     9061 gtatggtcta gtcttgtatt ttcctcaaga agatgatgat tttctctttc taattcgtca
     9121 tttttctcag aaagctgctt tgtgacttca gccatgggac gaatcgtttc caacaattct
     9181 ttcattgttg gtgtcaattc cccgataaag cgatagtgac aatggatagt cacttctttc
     9241 ccagaaatat ccgttagaac gaactctggt aattctttcc catcaaacaa ataacaatca
     9301 tttactgttt tgactacagc attcgaatcg ataaatgtta gtttttctcc ggcagcttct
     9361 gcgctgaatt cgataaagct aggttgttgc gcgaagcgaa acaccattct ttctgttgtt
     9421 tctgatacgg ggaaacttag tgtctcattt tcccctgtga aattattgaa tggtatttgt
     9481 tggattgttt catcttgttt cgtttgctgg gttacttcta acacttttac ataattagaa
     9541 ttttgtggct ctgcaatgga actttgtgct actggatgtt tcatcaatga gaaaaagtat
     9601 tggtagactt caccaaatgg tcgcattttg aaatcatagc gtacagcttc cggcaattct
     9661 tcataagttg attttaattc tgtatcacct acagctaagt acaaataatc ttcaatatta
     9721 atgaataatc ccgctttttc aaaaactttt tggaagccat catgagtata aaaagaatta
     9781 tgggtttcat ctaggagacc ataagaagcc cacggcaatt ggttattgaa caaatcgatc
     9841 atgacagaat tatgcgccaa gttagggaat gtaatcagga tctctccttc ttcattcaaa
     9901 aattctctga ctttcttcaa tgtttttcca ggatctacca aatgttctaa aacatccgca
     9961 aacaagacgt aatcaaatgt ctgtccagca taatagtttg cccactcaaa actttcgata
    10021 tcaccataaa atccatcttg agcaaattcg ctgacaaaat cgaacaattc cttatctaac
    10081 tcaacaatgc tcacttcaca ttgttttgct ccgattaaat gtttcgttaa tcggccgttt
    10141 ccgggaccaa actctaaaac tttacttcct ggtttgatct gtgcggcaat ttttcccaca
    10201 ctgctttgtt catctaggtc catttcaaaa tcatatttca ttgctatgag tcccttcttc
    10261 ccatttatgg tctaagacca cgatgccttc tccgatataa gaaccgctga caaacaagtt
    10321 catgtacttt gtcttatata ccaaaggaac cgttgcattt tcttcaaaga ccgcaacgtc
    10381 aaaataatat tcaccagcta acaaacctaa cttttcatac tccagataaa agatattctc
    10441 tccttttttc caaggaatct tgataccatc tagtagagta ttaagtccgc atacataaaa
    10501 attgtcgact gttcgtagcg caattcctaa aaccggattt ttgattgtat catcttttac
    10561 ttcataagtt actttaacgt atagtttttc tccttgttcc agcatatcca ccggattttt
    10621 gtctttatcc aacaattccg ctttcgtcac gtcgacgata tctggagcgg cgaaccgttc
    10681 tacttggttt ttattccgat ccactgtttt aactgatttt ttctttaaaa agttttcata
    10741 attcgtcgtt acttccatcg tatcgccata ttcgacgact tctccgtttt tcaaccaata
    10801 ggaacgatca caaaaacgac gaatcgaact gatatcatga gaaacgaata aaatcgtctt
    10861 tcccgagttc ttgatttctg taaatttttc catacatttt agttgaaatt caagatcgcc
    10921 tactgccaat gcttcatcca caatcaaaat atcgggattc acattgattg ctacagcaaa
    10981 agctagacga acgaacatcc cgcttgaata agtttttaca ggttgataaa tatggtctcc
    11041 gatgtcagca aattcaatga tatcatcgac catttcatcg acttcttctc ttgaaaatcc
    11101 tagaaccatt ccatttaaat agatattttc atagccagaa tactcaggat tgaatcctga
    11161 tcctagttct agcaaagctg agatttttcc ttcgatttcc atcgtgccgg atgttggtgt
    11221 cagtactccc gtaatgattt tcaacattgt agattttccc gatccgtttt ctcccacgaa
    11281 accgatcatc tcaccttttt ttatttctac attcacgttt ttcaacgcat aaaatacatc
    11341 atgataggat ttcttaaaag gattgagggc ttcacgaaat cgatccgaag gttttgcgta
    11401 catattataa gattttgtga tatcttttag tctaattgca tattctgtca tagctattct
    11461 ccttacagca catctgaaaa atgtggtttc aatctacgga acactgcaga gccgattacg
    11521 aacattacta acgtgaaaac ccagaaatac aaagtatatg ttccatgctc ccagaaccaa
    11581 gcttcactta aaaatgactc acgataacct tgaacaacat agtacagcgg atttaatttc
    11641 aatatgctta caagacctga atcaaactga ttagggctcc ataatatcgg taatgtccac
    11701 atgatcaact gcagcacgat cgtaattaat tgcatgatat caggtaaaaa tggttggatc
    11761 gatgccgtca gccaagtcaa tcctgtcaag aaagcacaca tgcaaaataa ataataaatc
    11821 agctgtaaag acattgctga aggatatata ccatttataa gcgcgacagc tatgccaatc
    11881 agaatgaaga ataagtgggt gtacagattg gataggattt tcgtcgtggg caagatacga
    11941 atattgaata cgactttctt tactaaataa gtatattccc gaaaaacaac cgtagttgat
    12001 gcaaatgcat ctgagaagaa gaaccaggga atcatcccgg taactaaata tacgataaat
    12061 gggtattttg catcgatcat ttgtgctttc agtccaacac caaataccag ccaataaatc
    12121 aatacagtaa atagcggatt gataaacgcc cataagattc ctaaaaacga tcctgcatat
    12181 cttgatttga agtcattgat cgaaaactgt agcaataatt ttctgttctg ataaatgtcc
    12241 ttcataaata aatacaaatc tttaaacatt tgtgttcctc caagcgattt tcagtgaatt
    12301 acagtcaagt gccattttac catatttttg gtaccgagct gcgttcttat ttctttctta
    12361 attttctatg atacaagatt ttctctaacg tttctatttt acagaagatg ctagacaatt
    12421 gcaataaacc aaaaaaggct gcgacggttg tcacagcctc tctcgtattt tgtgcctatc
    12481 aatgatagcg ttggatcctg gaagaatgag cgctatctgg ttgttcctca taagatgtat
    12541 catcataagt catatcgtca tatgcctctt ccataacata gaattcctcc gcaacttctg
    12601 gttcatcata tcgatgaacg gccatagcca acaggatata aataatcgga acccatgggt
    12661 atgaagtcaa tgtatcctcc ataaatgact gcatcatcac cgcaaaaaag gcccagataa
    12721 aagtcatctg cccgttggat aaatctgctt tcaacgttaa gcgaccaata aaaaggataa
    12781 gcagcaaagc agcaatccca aatcctatca atccatagat attcaaaata gaattataac
    12841 tattgtggtt tcctaaaaat tggtttcctc gttggaagat aaatgttttc attcctagta
    12901 aaatttgttc agatttttct cctagcgtct gatagaattt atgccaaata tcttctcgac
    12961 cggtaaacaa attgactttt tcagagacag ccgccagata agaaatcggc aaagctaaaa
    13021 gaatcgtaac cgtgtaagca ataaatggcg cacgaatgac acgcatcaac gatttaggta
    13081 aaagatccaa tatcgcaaaa acaatcaacg cgactaatgc acctctagat tggcagaccc
    13141 aaatcccgag aaaagctgca acaactgcta ctgcaccgat gattcgtaca taccatcgtt
    13201 caaaagaact ggcaaatcca ctgatcaata aagcaagagt catcaaagaa cttccgatta
    13261 tattcgtatt gatccaaata tcagaaagcg aattgcctgg agcccagatg ctttgtcctt
    13321 ctggtacttg gatctctgta aatacacgat ataacgtgat tccgaggaag accgaaaaga
    13381 caatcagata aataccgcga tcccattttg tccactcgat ccatttaatt actaaacaaa
    13441 ataaaataat caaaggaaac agtaagtctc caaataaaga ctcatttctt aattcccgaa
    13501 agtaagaaaa gaaaatgaaa agggcaagcg caacacctag ataaagaaaa tcgacgcctt
    13561 ttgctttata aacatttaaa atgagcaaaa tagccacact aaaagcgata agtaatgaac
    13621 tattgttata tataaacaaa gttgctggaa aacctaaccc tgctgtccaa acaaaaaaga
    13681 ctagcgtcag taacaaaagc aagaaaccga cactcatttt gttgacaaat ttttgtacga
    13741 ttgcttgaat tatctccact ctcctttcta ctaacatttc aggattattc tcagctagcc
    13801 tattactgta cgactagctg attttcctga gtttgtgttt gtggtacatc tggataatcc
    13861 ggatattcta ctttaaaaat catataaccg tctttatccg gtccatatag ctgcgtgaac
    13921 tgcgcttcat ataattggtt gtaatcagtt aatggtcgtt tactgatcac atagtcgatc
    13981 cctaattttt ctgcgtcttc aaaattcaac gtgaccgaaa acatatctgg acgatctaaa
    14041 atgaactttg tatcttcctt agcgataaaa gctcttgtat gagcataccg attataatac
    14101 ttctcgtatt cttctttcgg atcgattttc ttccatgcag ctatatctgg ataaaagcgt
    14161 actgtattga aggatttcac tcccaatgtt ggggtgaagt tatataattc atcttcagac
    14221 aaccaaactt taccaggatc ttgttgatcg attttttgga tttcttttgc cagtgttttt
    14281 tcaaaaatcg ctcctgtacc ttctacgact ggattgacga aaaagccaga tacgataact
    14341 aaggcggcaa aagcgaggta aaacaagcgt ctccagccaa ataatgcaaa agctaaaatc
    14401 actgttccca acacagcaac tgcgatggtt tcaaaatccg aaaaataatt atccattttc
    14461 gaatgcgtaa tcgcatagaa ataagaccat gcatttactg ccaacaaaat gaatttatac
    14521 caaataggaa taacatcttc tctccaaata taagcgataa accagatcga taataaacaa
    14581 gccgcaaaac taaaagtaag gatggctctt aatggaggaa cataagacag cagagtgatt
    14641 tctgctagcc attttggcaa cccaacggta atccaaaaga tagaaaacag acaaaaaatc
    14701 atcaaaatac gtcccagtaa cttttgttcg ctatcttttt tgccaaataa cacaaatgga
    14761 cttgctagga atacagttgg aaaaaagtga tagaacaggg ccacttcaga gttattcgaa
    14821 tagttgatat cttcaaaagg aattttccaa tttgtaagga aagaaaaaaa ttcgccaatc
    14881 gtccactttc ctccagtact tacccgtttt cctggatata aggtattcaa agaagctaac
    14941 aatgcgtctt ttgacgttag ccagaaatga accatgactc cgccaataaa taaaactgcc
    15001 cctccaatca acactacatc caacagatcc catgaaactt tcttgctata gtaaataagc
    15061 aggccaacaa aataaaaaat cagcaagtac gccagcatca cttgatgggc tgggtaaatc
    15121 actaaaataa acccaatacc gaaaataacc accaaaagca tcatcaacgt tcgtagccat
    15181 tttttatcat gattgtagaa ataatgataa aaagcagtca tcagtcccaa agtaaagaaa
    15241 atcaaatcgc ccacatgttg catgaaccac cactggactg ctggagaaaa tgtcagcgca
    15301 aatgcaccga ttagcgatag agcttttttt ctcttcgtta atatcatcag tacttcgaat
    15361 ccaagcaaga ccatgacgac taatttcatc gcccaataaa aggaaagtcc tcgatctctt
    15421 ccgaggaaaa agaatcccca gttgaatggt tttccgataa ccgtaatatc ctttacagga
    15481 gaattatacg caatgatcat gttttgtccg tttaatgaat aatcctcgtt caccaacgga
    15541 aagtcctctt cagcttgtga gaggtaaaaa ggtgtctgca ccagccattc gtctgaccgc
    15601 acttcccgat tctgtccaaa aatcacatca gtttttttcc catcgtctct ttgcgacaca
    15661 tatttatccc aaacaccaag tgaacttccg tttaaattaa aagtaacgat gaccacgatg
    15721 actaaaacgc caataagata gcggagtttt agaaacaggt caagcaccaa attcgaatct
    15781 gcctggttcg cttttggctt ttcttttcgt cgtgcacttc ttgtttgttt ttccatatca
    15841 tgctccaaat tctatttgat aaaaggaaat tacttattca tttttttggc agcttccagc
    15901 atttgcgcaa gtgcttcttg ccaagttggt atgacaaatc ctaactcttc tgtctttttc
    15961 aggctcatga ctgaatactg cggacgtgtt gctttttgcg gaaattcttc tgacgtaaca
    16021 ggtgcaactt ctacatctgt atctttcaat atttcttttg cgaattcata ccaagaacag
    16081 ctattttcgt tcgacaaatg ataaatacca aatggtgctt tttcttcgat gacatatacc
    16141 ataaattctg ccaaagtacg tgtccaagtt ggtcgcccaa actggtcatt tactacagtc
    16201 agtttgtcgc gggtttccgc taggcgttgc atcgtgaaga cgaaattgtg tccatagatc
    16261 ccgaaaaccc aagaagttcg tatgatataa tagtcttcta aaatctcttc tactgctttt
    16321 tcgcctaata atttcgtacg accatattca tttagtggat tcgtttggtc gtctacttcg
    16381 tattctacat ttttgttttt tccatcgaaa acatagtctg tactgatata gacgaattga
    16441 gcattcacag cttttgctgc aagtgctaca tttcttgtac catcaacatt gatcttctca
    16501 tctaattctt ttgcttcttc ttctgcttta tctactgctg tgtaagctgc acaatggaaa
    16561 ataacatcag gttttaattc tgtgatgtaa gccattgttt tctcggcatc tgtgatatcc
    16621 aagtcttttg catctgttga aacatattgc agattttttt catctaataa atggcgcaat
    16681 tctgtaccta gttgcccatt tcctccagtt aataaaaaca tatgattatc cgctccttga
    16741 tttttatttt aaaaaagaga caggcgagcg cctgcctcac atgtgttttt ccattaatac
    16801 agtgacattt tctctgtatt attgtccatt tttggcatat gctgcttcaa cggcctcttt
    16861 ttcttttttc caccagtctt catgttcttc gtaccatttg atcgtgtcag ccaacccatc
    16921 acggaagttc gtaaactcag gcttccatcc taactcttcc cgtaattttg tcgaatcgat
    16981 tgcataacgc aagtcgtgtc ctgcacggtc gtttacatgg tcgtacgcat cttttggttg
    17041 acccatcatt tctaagatca attccatcac ggtcttatta tcttcttcac cgtctgcacc
    17101 gatcagatag gtttcgccaa ttctgccttt cgtcaaaatc agccacactg ctgaagaatg
    17161 atcattcgta tggatccagt cacgaacatt ctttccttcg ccatataatt ttggacgaat
    17221 accgcttaag atattcgtta cttggcgagg aataaatttt tcaatatgct gataaggacc
    17281 atagttgttt gaacagtttg aaatggttgc acgtaagcca aaagaacgca cccatgcttt
    17341 tactaacaga tctgaaccag cttttgtaga agaatatgga cttgaaggat tatatggtgt
    17401 ttccgcagtg aatttttctc cttcgccttc cccatgtcct ggcaggtctt cacgtaatgg
    17461 aagatcgccg taaacctcgt cagtagatac gtgatgatag cgtacatcat acttgcggca
    17521 agcttctaaa agtgtatacg taccaatgat attcgtttgt acaaatggga aaggatcttt
    17581 taatgaatta tcgttatggg attctgccgc gtaatgaaca actgcgtccg cttcttttac
    17641 tagacggtcc actaattctg catctgcaac atctcctacg accaattcta cgcgatcgct
    17701 aggaagtcca gccaagttct ctttgtttcc tgcataagtc agtttatcca aaacagtgac
    17761 atgcacttct ggatgattgt ttaccacgta atgaacgaaa ttagagccga tgaatccggc
    17821 acctccggta acaatgatgt ttttcataag aaaatttctc cttaattctt agatttctcc
    17881 gtaaacaaat ggattttctg cttcaaattc ttttaatgtc ggatgacggt gatctttttc
    17941 cgacataatc gctcttgatg gatcaattgg ccaatcaatc gctaaatctg gatcatcaaa
    18001 tgcgatgccc ccatctgctt ctgcattata gtaattatca cacttataaa ggaagttcac
    18061 atttggtgtc aaagtaacga atccatgggc atatccttta ggtacaagca actgtcgatg
    18121 attatgttca gataagataa atccgtccca ttgaccataa gtcgggctac ctttgcggat
    18181 atccactact acatctaaaa ctgctccagt gactacccga atcaattttg tctgtgcagc
    18241 ttctcctttt tggaaatgaa gcccccgaag gacaccagct tcagctgata gtgaatggtt
    18301 gtcttgaaca aagtcatatt tcaatccatg ttctgcaaat tttgcttttg aataactttc
    18361 tgtgaagaaa ccgcgatgat cgccaaagac gtccatttct acgattttga catctgttaa
    18421 ttttgtatct gttactttca tctgtaagga ctccttattc agcggccaaa ttcaataaat
    18481 attgaccgta ttgatttttt ttcaatggct gtgcaagggc cagcaattgt tccttatcga
    18541 tgtagcccat acggtaagca atttcttcca aacaagccac ttttaagttc tgacgttttt
    18601 caattgtttc gataaaggta gatgcttcaa gcaatgattc atgtgttcct gtatcaagcc
    18661 aagcaaatcc gcgtcccata agttctacgg atagttttcc tttatccaaa tatgctttat
    18721 tgacatccgt aatctctagt tctccacgtt ctgaaggttt gataccttta gcgatagaaa
    18781 ttacctcatt gtcataaaaa taaaggccag ttacagcgta atttgatttt ggctgagcag
    18841 gcttttcttc aatcgacaaa gcttgcatat tctcatcaaa ttctactaca ccaaagcgtt
    18901 ctggatcatt gacatgatag ccaaatactg tcgcgcctgt atcttttgaa gctgctcgtt
    18961 gcaacatttt agacagaccg ccaccatagt aaatattgtc ccctaatacc aagcagacac
    19021 tatcatctcc aataaattct tctccaatga taaacgcttg tgccaatcca tctggacttt
    19081 cttgtactgc atattcgatc tgcagtccta aatcatttcc gtttccaaat aattccttga
    19141 aacgtggtgt atcttgtggc gttgaaataa tcagaatttc tttgattccc gccaacatca
    19201 gagtagacat tggataataa atcattggtt tgtcataaat cggcatcaat tgtttcgatg
    19261 ttgcctttgt taaagggtac agacgtgttc cgctgccccc agcaagaatg attcccttca
    19321 tggatatctg ctctcctttt tgatttcaaa tttcactttg ttattatctc atcaattgtt
    19381 atatttgtca tggaaatatt aaggatttaa acatctttta tcaacgttta aaccattttc
    19441 gtttgatcaa cgccgtaatc agatgaacac gccaagaaat tttttgccgt aatttagaat
    19501 gtgtttcaat ctcactcgcg tttaaatcat gtcttcggta cagagttaac ggttctctta
    19561 ggaacccagt cttctttcct cccagcaaac caatccacat atcatgcatc ggcacttctg
    19621 acgggatcgg caaaatatcg ggttttaaag aagaccggaa agccatacca gcaccaatat
    19681 agtgacttct tatcacatta ttaagccatc cttctttgga atttcgaaac gaaaaatacg
    19741 aatcatggat ctcttgcagc tcgctgtcca caatgattaa atcagaaact actactagct
    19801 gatccggatt ctgctcaaaa tagtcagtga tctttttcac ttttgtctcc aaccaaacat
    19861 catcttggtc tgccaaaaag attagatctc ctttcgtctg ctggatcgca taaccaaaat
    19921 tagcgatcaa tccttgacga ggccccgaaa aaagctggat tcgcttatct ttttctgcgt
    19981 attcagttaa tatatcccat gtatcatcta ctgacccatc atcagaaatg actacttcat
    20041 cttctgctga aagctgagat aaaatactat ctaactgctc tcttaaatat ctgcctccat
    20101 taaaggtagc tatacaaacc gaacgcacga attcacacct tccaattctt aacatatgag
    20161 cgcaaagtca tccgccagat atgccggttt ttgacggtca agaactgttt agctgttctt
    20221 aacaataatt gtcgtcggtg actggataaa tgcctttgat catattcatg ataaaatcga
    20281 ttttctgctt gtaaaataga acggtatcga ttacttgaaa ctttttcata gtctaaaacc
    20341 gaaagatcat gttccattcg tacaggaagg atctccacag ttttttccaa ctgatccact
    20401 ttccaaaaca gccaatgatc caaaaaatct aaagaaaaag caggattaaa cccgccaatc
    20461 ttttttagaa atttgacaga ccaggcaaca cctgaattga ttgccatgat tcgttcagct
    20521 gttgggcctt caacttctac tgcttgagct gtacgattga tataacgatc tgaatatagg
    20581 ggagaaactt gtcggctccc agaaaaaatc atcggaactg cagcaacaca ttcatctgta
    20641 aaggttactt tacgcaatag ttcaaaataa tcgtcgacta gcctcgtatc ctgatccaga
    20701 gtgacgagac aacgtgttcc ttcatctgct tgagacaacg caaaattata agccaccgct
    20761 aaaccaggat tttgcggatc atgatgataa acgatatttt ctttttcaaa cagctcattt
    20821 tgctgccttt gaggactatt atcgtaaata ataagctgaa tttgcttttc gctaagtgct
    20881 ttgcttaatg gtccataagc tggagattga ctgaatagtt gctgataaag cacaacgact
    20941 actaatattt ctggtttcat cagaaaaaac gccaccccca cttattgaaa aatttcatca
    21001 tagaagtcaa aaacacgcga aacagttttg catttcgatg cgcacccttc ccgtaaaaat
    21061 ggataactgc ataaaagggt gtgtacaata acttgtaacc tgctttttca aaactcaaac
    21121 acaagtcgtt gtcttcaaag tacatgaaga aacgctcatc aaaccccttt atctctttaa
    21181 atttatcaat atcagctacc ataaaactac ctgagatcat ccgtacataa ctattagcat
    21241 cagtaggcaa tcctcggcat tcaaacacag ccagtcgttt gtcaaacttt tttttgaccc
    21301 atttgaaagg cacaaagcgc aaaacgtaat caaaaacagc cagctgctca cggatgaggt
    21361 gctgagttgt tccatcttca ttaagaatct taggtgctaa catcgcacat tccggatgtt
    21421 ctttcaataa caagaccaat gctgtaactg tctcttcatc cactaaaata tctgggttac
    21481 aaataatgcc atagcgagaa tcgctagcta tcagattgtg attatgtcca taaccaaatc
    21541 cggaattttc atcatgaaaa tagatatcga tgtagggata tttcctatac tctgttagtc
    21601 tttttttgta ctcgggaagt gaattgttgt caaaaattcg gatttttact gctgtttcat
    21661 cttttacagt tgaaataaaa ttatctagtg tccgaaaaat tttatcactg tttgccgtca
    21721 caatactaat aaggatcggc tggtttaatt tgttattcat ctatatgacg gttccatttc
    21781 ttcaaatatt tctgttttac ttgcgtacgg tactctttat tccctaaaaa tcgcaatgtt
    21841 tttaaaataa actgatgttt ctcattgatc agaccaatca attcaaataa taaaattgca
    21901 gcaaacaaca tggcaataat caatatgatc gttccgattc ttccagtata attcatgacc
    21961 atagctgtga aagaaaacat caaagccatc gcataaatcg tcaggacagc acctttatga
    22021 gtaaagccta aactgattag tcggtgatga agatgttttt tatctgctgt agaaaaagaa
    22081 acacggtttg cttttctcct aatgatagca aaaaacgtgt ctgttaccgg cactcccatg
    22141 acgatcagca aagaaatcga tgaaacaaaa gtcacgttct tcagcccttg tagtgaaagg
    22201 acggccatca taaaccctag gaatagagct cctgtatcgc ctaaataaat ctttgccgga
    22261 taaaaattgt aaggaaaaaa accgataata cttgctacaa gacagaaaat aacaactgga
    22321 atatacactg tagaagcatg gaggaaaaaa tatccaatga ttcctatcgt aattaaaccg
    22381 atcaatgaaa tccctgccgc cagcccatcc aaaccgtcaa tcagattgac cgcattcgtg
    22441 ataccgaaga tccaaaaaat cgttacggga aaactaagcc agcctagttg gatatatcca
    22501 ataaaaggaa gcgtgaagga atcaatacgt atatctgcta caaaataaat aataagagaa
    22561 gcaatcaaga tccctatact tttttgttta ggtgtcagct catatttatc atcgagcact
    22621 cctgtgacta tgattactaa agaagcaatc aagatcggta caatatacga ttgagggata
    22681 attttacgga ataagaataa agtcgatata acaaatgaaa taaagatagg cagtccgccc
    22741 gctgaaggca ttggaacttt atttattctt ctttcatttg gtttgtcgac catacctgtt
    22801 tgtactgaaa tgattttgaa tacaggtgtt aatatcagtg ataataaaat tgtcagaaaa
    22861 aatcttataa ccatactaaa agaaacaagc atatgggcac ctactttaat taagagtcat
    22921 aattattata aattatatat gtctttttga ctatcttttt atatcttctc aaatgtccgc
    22981 aaagccttat tttatcggct ctacgggcat tttgcttttg tggtaaacct cgcatatcta
    23041 ggtctatctt cttatatttt ccctgtcaat cgtggttaaa atcgtggtaa atacttctat
    23101 gcgattagac gctgaacctc tgattttgcg gtatctatgg acgcatgagc gtaccagttc
    23161 attgtgatac tgatgtttga atgccccatg atatactgta aatctttcgg gttcatgttc
    23221 ttgcttgcca gccttgtgca gaatgtatgg cgtagcgtat gcggtgtaat atgtggcaag
    23281 ggattgtcct tgtggtgctt gttgtatttc tttaccatac ggataaataa ggcgttataa
    23341 tcaatggcaa cttttggatt acctttcgga ttgataaaca caaaaccgct gtgtccgtct
    23401 acctctatcg actttatctt tggtcggctg ttgataaccc gttgaaatgc ctgtatcgtt
    23461 tctttgctta atggcacttg ccttatcccg cttttcgtct taggcgtttc aatataatag
    23521 ccctgttcct tactctttag taactggtgg tcgataatca caacttcatt cttgaaatca
    23581 atgtcggcta ctgtcagtcc gcacagttcc gagatacgaa gtcctgtctt taacagtatc
    23641 agcacatcat cataatgctt gtgatacacg ttgtccgtct taataaatga caataaggct
    23701 tgctcctgtt cctctgtcaa tgcgactttc tctttggtat cattttctat gacttcactt
    23761 agcttgaaat caaaagggtt tttcctcaca taatcatctt gtatggctat gtagaatgac
    23821 gcttttaacg agcgtttatg gttgttaatg gtattgtagg aaaagccttt gtctttcatg
    23881 cgtaacgccc attctttagc gtcagagggt tttatcgtat caatgctcct agcacctaac
    23941 ttgtcctctt ttaataaccg catgagttgt tcccgttgtt tctgtgtgct tttcttcaca
    24001 tttgtcctct gtgcgttctg tttggcatag agttggcaaa gcgtcatttt cttgcctgtg
    24061 ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt ccagttctcg aagtgagggt
    24121 ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca atctccaagc atacacatat
    24181 tttgtgtttc ccaatgcgtc tacatattta tataagtatt ttccgtctgt tcgttggctc
    24241 tctccagtat gcaggatacg acctttgtta tcccgtcttt tttctttcat ggtgtctgct
    24301 cctttccttg ttggaaagag ccttgatatg acttgtgttc atcataacac atacaaggct
    24361 catttgcatt agattgcgtc caatttatca ataacctttt caaactgtcg gcgtttaatc
    24421 tgtatgcggt tgccattcat aatgagccag ccagcgtcct tgttttcctc tgccaatcgt
    24481 cttaacttgt tttctccaat gcggaaatac tttgacgctt cttcaatggt aagggtgtat
    24541 ttttcccata cgggaatatc ctgtgtatta ctcataaaac accccctcac tttcgattac
    24601 gtctgatatg tccgtacaca tctgttccac aagcatttcc tgtttttcag aaaggtgggt
    24661 acattcatca atcattttca tgtagtttga acctgtcagc ctgtcgattt cctctatcat
    24721 cacgattgac ggcatgacct gtctttccag ccattgcaga gtgcgttcca tattgacagg
    24781 tacagggttc atttcaaaag gtaaactgtc atgggaaatg aatatctccc acaatggata
    24841 gtcaggaaat tccacaaaat cggtaatgag ataaaacacc agcccatgag ggttatacgt
    24901 caataacagt tcttcaactg cctgtaccgc ctttttgtca cgcagacgaa tttcaaaacg
    24961 gttgataata tcggtatgtt tctttttcgt tgcctgttcc ttttgttttt catacaggca
    25021 gaaatatttt gtgcttgcct ttgagccgat ataaagggta cttgctaaat tacggttttt
    25081 tccgcttaat tttccacctt gtacattttc atagttttta cagcgacatt ccgcaccacc
    25141 atttctgtat ttttcagata gcacaggaat atccagcaga ccgcacatat cattgattgc
    25201 caagtcaaaa cgtcggataa caccgccaca gtcaagacag cggtttaaga atgaatacca
    25261 gtccctgttc tgtgcctgta agacatattc catgttgcgt gagcctgccc cttttaattc
    25321 taaaaatacg cccatgtgtt catcatcaga agccatgacc ttgatttccc cgtaagcata
    25381 atgctcctta tagccataat acccatagtc ataatggata aaataatctg ctttcatgcc
    25441 aagcaccttt cggataactt ccaatgcgtc agtcgttgga aaacggacag acagataatc
    25501 aacgagcaga taaaaaggct tatcgcagat atagtaattc agacaacgca ggataatgtt
    25561 ctgtgtttcc ttgctggcgt tagcttttcc gttctcaaag cggttgatac tatatcttga
    25621 tacatgggta agacttgcaa gttctccttg tgaaagaccg ctggcaagac gtttgttctt
    25681 catttcaagt gcaaattcta aattgttcat tgtattcctc caatcccaca atagccttga
    25741 aatacctgta aaccaccttt caaaaagtgt gatatgtact atcttcttaa aacctctcat
    25801 tactactcac aggtttgggg gtgtatggtt tttgacttgt cttttgtgaa atagtgcccc
    25861 cctgttagat aacgggggca cagacaaggc tcgcaggctc gccagccgac aaagcatttc
    25921 ttccccttga cacaaggtgt catggcggta agcctgagag gcttccgcca ccgtgtcaga
    25981 aatactttgt cagcaagttc tgttttgcct tttctaacaa gttccatttc aaccgatatt
    26041 catatctacg gatttaatca gataggtatt tctttttgaa tgtttttata tttcattcta
    26101 ttgtgcttat tcgattacaa gaacatagta attcttatat gcttatttgt caatatcttt
    26161 ttttgccttt acaaatcata ttaagcatga tagacttata atagcgaaga tataacgaga
    26221 tatggagggt tatgcaatga aaataagcca tttaggaaat aatatacaga ccataagaaa
    26281 atttagggga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata tgcagagcct
    26341 ttccaagatt gaaagagggt tgaactatcc tgcctatgaa acgctggaaa agataatgga
    26401 agtgctggac gttacgccca atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc
    26461 tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca
    26521 tggacactat gataacttct ttgacagcga ggaagaatgg ctggaatatg agttagaaaa
    26581 gttacgggaa tatattacag actatatcaa cgggaaaagc attgaagcgt ccgaccttta
    26641 cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa ttattagacc gctatgatga
    26701 tttatacagt atggatatgt ttggggaaag catagaggga cataagtata agacacctta
    26761 tcaagtcgtg aaaatgataa acccgaactc aaaagaagat atggaactgt tacgagaagt
    26821 attaaggaat aacaattttg atgatgagga cgagtaactt ttttcctctt gcgagaagat
    26881 acagcaaaca aaaagcccga tcaagagtgc aaagcaccac caatggtggc aaagctcttg
    26941 acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt
    27001 tgtggtaaat caggcaaagg caacgcaggt atcacagtat gaattgacac cgcataagga
    27061 aagcaactgg aagattgtag aataaatgaa aaaggcttgc attctgcatt tttataaaat
    27121 tgcaagtctt tatattgaca agcataaaat actaagatat actttaatca gaccaacggt
    27181 ttgtattgga ggtaagttat ggcacgaaat aaatatccag aagaaacagt aaaactcatt
    27241 ttagatgttt ctacaaaaat gttttgtgaa aaaggatatg ataacacatc attgcaagat
    27301 attatcaatg aaacaaaact ttctaaaggt gctatttatc atcatttcga ttcaaaggaa
    27361 gatattttaa aagcaatttt tcaaagaatt ggaaatgaaa atgcagtaat atttgctaaa
    27421 attagagata acgatagttt aaacggttta gaaaagttaa gagaaatatt taagacagcg
    27481 gtattcaatt ctaaccaaag cgttttattg acagtttctc ctcaactatt aaacaatcct
    27541 cgttttcttg ctatgcaaat agaacaaatg tatgaaattg tagtacctga atttgttgaa
    27601 cccgttttag aacaaggtat caaggataag tctttggaga ttgaaaattc acatgaaatt
    27661 gcagaagtta tcatggtatt aagtaatgta tggctcaacc cattagttga aatgacggat
    27721 aaagagggta tggaaaaacg ttgcaataca ttcaatgccc ttttaaatgg aataggcata
    27781 gacacattat tagacgaaga aacaatttct gcatttatac agttttgtaa ataactgtat
    27841 ttgttatccc tatacgatag actgctctat aaaagagcag tttatttaaa ccattatata
    27901 aaccgacggt ctgtttgatt aaataaaaag gagagatttt atgcaagcag aaaaaaaact
    27961 ttttaatcgt gattttatac ttgttgttat aggacagata atttctttat ttggaaacgc
    28021 tatcctacga tttgcattac cgtactattt gttaaaagaa actggttcat cagctttgtt
    28081 tggtattgct acagcctgct cttttttacc tatggttatt ttgtcgctgg taggtggtat
    28141 tattgctgac aggacaaata aaagaaacat tatggtcata ctagattttg taacagcaat
    28201 cataatttta gtattttatt tgtcattggg aaaaattcct atcatacctt tgtttattgt
    28261 tgctttgatg ttactttatg gtatttctgg tgcatatcaa ccagcggtac aagcaagtat
    28321 tcctctattg gtatcatcag ataaacttac cgtcggtaat gctataataa atcaagtaaa
    28381 tactttagca aactttatcg gaccagctat aagcggagta gtttttcaat tttatggtat
    28441 caatcccatt ttaattgtta gtattatatg ttttactttt tcagccatta tggaaatttt
    28501 tatccatatt ccacaccaaa aaagacctcg tgaagtaggt gtaattgcag ttgctaaaaa
    28561 cgatttaaaa gatagttata cctttttgaa aaaggagaaa cccattttta tatccgtaat
    28621 ctttttggtt tgcattttca atttggtttt aagtgctgtt ttaattgttg gtctgcctat
    28681 cttaatttct gaagttttaa aaatgccaga tacactatat ggtttttcac aatcagcatt
    28741 atcattaggt ggtttatgtg gtggtatttt aacagctctt gttgcttcaa agttaaaact
    28801 aaaaaacctt tataccttgt tacttatttg ttctgtttct gttttactta tgggaatttc
    28861 gctattttta gaattgcctg caatagtttc ctactgggta gttacagtga tgtgctttgt
    28921 ggctatgggt gtatctacta tgtttgttgt tcaaatttac actcttgttc agacacaaac
    28981 accaccgcaa ttagtaggaa agataatggc tacgctggta gcagttgcta tgtgcggtca
    29041 acctattgga caaatggttt acggtatttt attcgatgta tttatacaag aaacatgggt
    29101 tgttatgttt gtagcttcta ttgcttcaat catgatttca atatattcaa aaagaatttt
    29161 tcaaaagtta gagatataat acaaggggtt aaatagtatg ttaggctaaa aacaatatat
    29221 aaaagaaaat ttgaaatctg ctaaaaggat agtgttataa aagaaagagt aatattgcag
    29281 tatttaaggc tcgcacaaat atatgttttg tgtgggcttt tttcttacca tttttctgta
    29341 tttctaaagg gtataaacgg tattccgttt atatatttca gcattagcag aaagggggtg
    29401 agattatgag gacatcttct ttcgagcata tcgtcagatt acagttcaat tctttaatgc
    29461 tgattgtcat taagaacaaa gtgaaaagtc gaaacagaca gcttgcaaga cattctaaac
    29521 gtgaagtatt gttttgtgaa atagccgaaa cgaaacaagt tgaacgtgga acgattgata
    29581 cctgttttta tgattgtgta tcatttaaag tcttgcactt tacaatttat gtatctgatg
    29641 aaacacttgg tacagtttta catagactgt cagaaaaaca gcgtagtgcg attttattac
    29701 gttattttca aggaatgaac gacagagaaa tttcagaact gtaccatgta tcacgttctg
    29761 ctatttgcag tagaagaact agaggactaa ggaagttaaa aatgcttttg agcgaaagga
    29821 agtaaagaaa tgagtaaaga ttatggctat ccctcttttg aattgatttg caaagcgtca
    29881 agcggagatg aaatggcagt taaagagatt ttgaagtttt atgacggtta tatttctaaa
    29941 ttatgcttgc gtccttttta ccactctgaa agtggtaaaa tcattatgca ggttgatgaa
    30001 gaactaaaag gagaaattca tacagacatg atgaaagcaa tattgaaatt tgaaatcgga
    30061 gtgaagtaat caactgtata aaaaaatcgg gagatacaca gtttcgtgct atctccctta
    30121 gtagttttac ggtcatatca aggctcattc aatcgtggta aattcgtggt aagatgagtg
    30181 tttttctata cttcaaaatg cttgaatgta tagtgtttat gcggataatt gaaaattaga
    30241 tataaaattt caaataaatt actgatataa atttacaata agaactcttg tttctacttt
    30301 tttttatgag aaaatttagt tttcaattta ttgaaccatt ttcggcttgg atctcgacgt
    30361 tttttgattt gatgacccgt cacgtattct tgaacaactg cattgatgat tccgcccaaa
    30421 atgatgatcg tagcagcaaa attaagccaa agcatgagta caataaaact tcctatgatc
    30481 tgataaccgc tgatcctcgt tgcaaaatac ctagcataga tactaaacac ttgtgataac
    30541 agcatccagc cgacagtagc gaagatcgtg cccggaatga tcgtcttcaa cttcaactga
    30601 gcattaggga caattaaata aatcaagaac atgatgacaa ataaagctac caatgtgatt
    30661 ggccacttca aagtttggaa ctgatcgata aagttcacgg gtatttgtac gattggctga
    30721 atagcatcca aaatcagctg tcccaaccca agaacgatag ttaccccact aatagcaatc
    30781 atgaacaaga caatgacaat tagtgagaag aaacggacga tcaaaaaatt ttttctattt
    30841 tctacgccat acgctttatt catcgcaatt tgcaaagcat tgatactttg acttgcagac
    30901 cataaagccg ctaatgcaga aacggataac aagcctcctg aactttgtgt caaaaggtct
    30961 ttgattgcag gaccgataaa ctggtaaacg gtctcaggaa tcacttctcg taaataggtc
    31021 agtacagtct cttgatcgat gtggagaaac ggtaaaatat taccaaaagc aataatcaat
    31081 ggaaataaag acaagagtaa ataataagca acgacgacag aggtcgtact catttctgaa
    31141 tcagtgatgc gcttttgagt cgtctcaata aagcgcatca gattttcatt ttgttttatt
    31201 ttgttccaaa ctttcatgta cctctcccgt acctattgac tggatagatc aataagtgcc
    31261 ttcttctcct tgtgatgtca aaattcttgg accatcttta gtaatagcca atgtatgttc
    31321 atattggcaa gaaagtccgc cgtctcttgt atacgcagtc cagccgttag gatccatttt
    31381 cattttccaa gttccggtat taaccatagg ttcgatcgtg ataaccatgc cttctttcaa
    31441 ccgcaaccct tttccagctt ctccgtaatg aggaactgct ggttcttcat gaatcgtagg
    31501 accaacgcca tgaccaataa aatcacgaac gatcgctaga ttttcgcctt ctacataagt
    31561 ttggatagca tgaccgatgt caccaatccg attccctact tgtgcttgct caataccgat
    31621 gtacaaagct ttcttcgtta cttccatcaa tcggtcgatc tcaggtgtag attctccaac
    31681 tacataggcc caacaagaat cagaaatgcc gcctttcaaa tcgatacaca tatcgacttt
    31741 taccaaatca ccgtttttca aaggcttttt acgagggaat ccatgacaaa tttcatcatt
    31801 tatactgata caagtcgcat attcataccc ttcaaagcca atttgcgcag caattccgcc
    31861 atgactctcg atatagtttc tgacaaatac ctcaatatcc caacttgtga ttcctggttt
    31921 tataaaatca cgcagatttt tatgtacatc tgctaataaa gctccagatt ctgccatcat
    31981 ttcaatttca cgtgctgatt ttaaagtaat catattttcg tctccttttt acaaatccct
    32041 ctcattttaa catagcggca tacatttagc caaatgacat tccccaggtg tctctaggtt
    32101 ttattttctt ttcaatgatg ttttggtata attcataatt gttaaataat tataaacgag
    32161 gaggcacatc atggctttag caaaaatcgt atatgctagc atgacaggaa acactgaaga
    32221 aatcgccgat attgttgcag aagcatttga aaatctggat atcgaagtcg aaatcaacga
    32281 atgtacccaa gtagatgcag atgaattcga ggaggcagat atttgcgtag tagctactta
    32341 tacatacgat gatggcgatc tgcctgatga gatcgtagac ttttatgaag acttgcaaga
    32401 attggatcta ttgggaaaaa tttacggtgt ctgcggatct ggtgatactt tttacgatga
    32461 attctgcaaa tcggtggatg atttcgatgc tgcttttgca aaaacaggcg catcaaaagg
    32521 agccgaaaat gttaaagtgg atctgaatgc tgaagaagaa gatatcgaaa atttagaagc
    32581 tttcgctaaa gaactagtcg caaaaattta atatacttgc aataaaagag ccatccagat
    32641 ttattcaatc tgtatggctc ttttattcat tgcattcatg gaagagttct tcgggagtta
    32701 aaaatgcata cgctgtaaga aaaacgaact tttccttatt ttgacacatc tcttgttacc
    32761 tactgatttg tggaactttt gagtatattc aatcgatttc gattggttct ttgaatttca
    32821 agatagcatc tatactgtct tggatcatct tttcaatctg ttcatcagtt ggatggatga
    32881 tgcttgaaga catcaaagct gcgatcccag tagctatgat ccatgtccca ttatacaaag
    32941 attcgatctg ctcttctgaa agattcacat aagctggatc gcgtttcact gtattcacaa
    33001 agtaagtata agagaaattc tgcatctctt ttcctccacc atattcttct aagtacagtg
    33061 aacgatacaa cttcttctct cttcttgcaa aatggatata attaaaagca agatcgataa
    33121 tcgtattccc tgtatgctca acagtgaaca cttcatattg taaatgatca tagatctgct
    33181 caaaaagagc ttgtctcaag tcatccatat ttttaaactc aagataaatc ggttgcgtgg
    33241 aacaattcat tttgttcgcg atgtttctcg cagtaaatcg tgtaaatcca tcggtggcag
    33301 ctacttcata agcagctgcc aaaatctgtt ctctagtaat cgtctttttt cttgccatat
    33361 tttttaatct ccttaatttt tttataacgc ttccaccatt gattaatttc attgtaacat
    33421 gtttttttga tatgcaaaat tagtttttga ttttcttcac aagctgaaat agcaagtagt
    33481 aacatatatc atgaaactta accttaaaaa aataacctcc tttttttatt taaagcctca
    33541 ttgtttgaat cataattaaa tggtatcatg ctaatagtta ccagaaaaaa tggtgaggag
    33601 gaatttttat tatgttatct gattttaatg aacttctaaa aaaatatgca cgcttaattg
    33661 cagaaactgg cgtagccaca gaaaaaggac acacagttgt tttacagatc agcgtggaac
    33721 aggcacccct tgctcgattg atcacacaag aagcttataa actaggagcc gctgaggtta
    33781 tcgttcaatg gactgacgac cagatccaaa gagaatttct tttacatgct gcaacggatc
    33841 gtattgagaa tgtaccacaa tctaaaatcg atcaagcgga tgaatggctt gaaaaaggag
    33901 caagcaggat cagcgttgtt tctgccgatc ccgatgcatt tgctggtgta gatagccatc
    33961 gagtagcgtc ttatcaatca gcgaatggca aagcactaat gaatttacgc aaggcaactc
    34021 aagcaaataa agtcagttgg actgtcgtgg ctgcggctgg taaacaatgg gcagcaaaag
    34081 tcttcccaga actgccagaa gaaaaacaag ttgaggcatt atgggatcag attttcaaaa
    34141 cgactcgtgt ctatgaagaa gaccctgtct tagcatggaa gaagcatgat gaaaaattag
    34201 caaaaaaagc agaagaattg aatcgagaac aattttctgc ccttcactat actgctcccg
    34261 gtacagatat cattatcgga ttgcctaaaa accatctttg ggaaggcgct ggaagctata
    34321 atgctcgtgg agaaaaattc atggcaaata tgccgacgga agaagtgttc acagcacctg
    34381 acagtcatcg agtagatggt tacatttcaa gtacaaagcc actgagttat gctggcacca
    34441 tcatctcggg tatgaaattt acttttaaag atggaaaagt tgttgatttt tctgccgaac
    34501 aaggagaaga tgtcttagca aaactgcttg atacagatga aggtgcgcgt cgtttaggag
    34561 aagtcgcact tgtacctgac ccttcaccta tttctcaatc tggcatcatc tttttcaata
    34621 cattatttga tgaaaacgca tcgaatcact tagcactagg ttctgcttat gccttcagtg
    34681 tgaaaggcgg tacagaaatg tccgatgaag aattagcaga agctggattg aatagaagtc
    34741 aaacccacgt agatttcatg gtaggatctg acaagatgga tatcgacgga atccgtgaag
    34801 atggctctac agtaccgatt ttccgaaatg gcgattgggc ttgagtatac tttcgcacac
    34861 atgcaaaaa
//
LOCUS       DAZKAY010000021        34662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000021 DAZKAY010000000
VERSION     DAZKAY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.021"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(313..2205)
                     /locus_tag="ACG8WP_000922"
     CDS             complement(313..2205)
                     /locus_tag="ACG8WP_000922"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027247.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="ACG8WP_000923"
     CDS             complement(2202..3929)
                     /locus_tag="ACG8WP_000923"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027248.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="ACG8WP_000924"
     CDS             complement(3945..4595)
                     /locus_tag="ACG8WP_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9027249.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="ACG8WP_000925"
     CDS             4799..5095
                     /locus_tag="ACG8WP_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9027250.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="ACG8WP_000926"
     CDS             5287..5907
                     /locus_tag="ACG8WP_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9027251.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="ACG8WP_000927"
     CDS             complement(5999..7165)
                     /locus_tag="ACG8WP_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9027252.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="ACG8WP_000928"
     CDS             complement(7177..7530)
                     /locus_tag="ACG8WP_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9027253.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="ACG8WP_000929"
     CDS             7658..8680
                     /locus_tag="ACG8WP_000929"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9027254.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="ACG8WP_000930"
     CDS             8699..8860
                     /locus_tag="ACG8WP_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027255.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="ACG8WP_000931"
     CDS             complement(9119..9553)
                     /locus_tag="ACG8WP_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027256.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="ACG8WP_000932"
     CDS             complement(9663..11522)
                     /locus_tag="ACG8WP_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9027257.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11523..11663
                     /locus_tag="ACG8WP_000933"
     CDS             11523..11663
                     /locus_tag="ACG8WP_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9027258.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11640..12800)
                     /locus_tag="ACG8WP_000934"
     CDS             complement(11640..12800)
                     /locus_tag="ACG8WP_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9027259.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ACG8WP_000935"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ACG8WP_000935"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9027260.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="ACG8WP_000936"
     CDS             complement(14547..15245)
                     /locus_tag="ACG8WP_000936"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9027261.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="ACG8WP_000937"
     CDS             complement(15202..16863)
                     /locus_tag="ACG8WP_000937"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9027262.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="ACG8WP_000938"
     CDS             17081..18481
                     /locus_tag="ACG8WP_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9027263.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="ACG8WP_000939"
     CDS             complement(18733..19812)
                     /locus_tag="ACG8WP_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9027264.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="ACG8WP_000940"
     CDS             complement(19898..20857)
                     /locus_tag="ACG8WP_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9027265.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22380)
                     /locus_tag="ACG8WP_000941"
     CDS             complement(20869..22380)
                     /locus_tag="ACG8WP_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9027266.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22966)
                     /locus_tag="ACG8WP_000942"
                     /pseudo
     CDS             complement(22402..22966)
                     /locus_tag="ACG8WP_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(22972..23835)
                     /locus_tag="ACG8WP_000943"
     CDS             complement(22972..23835)
                     /locus_tag="ACG8WP_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027267.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23849..24745)
                     /locus_tag="ACG8WP_000944"
     CDS             complement(23849..24745)
                     /locus_tag="ACG8WP_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027268.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24920..26209)
                     /locus_tag="ACG8WP_000945"
     CDS             complement(24920..26209)
                     /locus_tag="ACG8WP_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9027269.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26181..27698)
                     /locus_tag="ACG8WP_000946"
     CDS             complement(26181..27698)
                     /locus_tag="ACG8WP_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9027270.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27896..28786
                     /locus_tag="ACG8WP_000947"
     CDS             27896..28786
                     /locus_tag="ACG8WP_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9027271.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28859..29683)
                     /gene="nadE"
                     /locus_tag="ACG8WP_000948"
     CDS             complement(28859..29683)
                     /gene="nadE"
                     /locus_tag="ACG8WP_000948"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9027272.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29695..31167)
                     /locus_tag="ACG8WP_000949"
     CDS             complement(29695..31167)
                     /locus_tag="ACG8WP_000949"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9027273.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31804..32166)
                     /locus_tag="ACG8WP_000950"
     CDS             complement(31804..32166)
                     /locus_tag="ACG8WP_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9027274.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32389..33141
                     /locus_tag="ACG8WP_000951"
     CDS             32389..33141
                     /locus_tag="ACG8WP_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9027275.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33281..34204)
                     /locus_tag="ACG8WP_000952"
     CDS             complement(33281..34204)
                     /locus_tag="ACG8WP_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9027276.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34219..34491)
                     /locus_tag="ACG8WP_000953"
     CDS             complement(34219..34491)
                     /locus_tag="ACG8WP_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027277.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10231 a         7213 c         5979 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa aacagcacat gtagcaggca
    22861 cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag
    22921 aaaccacaat caactgtcca taaagtttag tctcaactat tttcatcttc ctcactcttt
    22981 cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag
    23041 taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg
    23101 ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat
    23161 caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag
    23221 agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc
    23281 aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa
    23341 gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc
    23401 aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt
    23461 cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata
    23521 ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt
    23581 atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt
    23641 atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga
    23701 agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat
    23761 cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga
    23821 gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt
    23881 acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg
    23941 atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg
    24001 tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca
    24061 tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg
    24121 taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag
    24181 agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc
    24241 aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga
    24301 tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca
    24361 tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac
    24421 gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga
    24481 taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg
    24541 ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa
    24601 agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa
    24661 ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat
    24721 tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata
    24781 caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat
    24841 aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta
    24901 tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt
    24961 tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat
    25021 cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt
    25081 gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat
    25141 ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc
    25201 aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg
    25261 catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg
    25321 tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga
    25381 tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt
    25441 ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct
    25501 caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta
    25561 acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt
    25621 tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt
    25681 gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt
    25741 gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt
    25801 ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg
    25861 ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt
    25921 ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca
    25981 tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata
    26041 tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat
    26101 cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca
    26161 aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga
    26221 catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct
    26281 tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt
    26341 gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag
    26401 tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg
    26461 tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag
    26521 tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata
    26581 aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca
    26641 ataacattta ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa
    26701 gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa
    26761 ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata
    26821 ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta
    26881 tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa
    26941 tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata atctatttga
    27001 tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc
    27061 gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt
    27121 tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc
    27181 atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc
    27241 cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa attacgagct
    27301 gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga
    27361 caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa
    27421 tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac
    27481 acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg
    27541 acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata aatcccttca
    27601 tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct
    27661 acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata
    27721 catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt
    27781 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
    27841 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
    27901 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
    27961 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
    28021 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
    28081 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
    28141 cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
    28201 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
    28261 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
    28321 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
    28381 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
    28441 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
    28501 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
    28561 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
    28621 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
    28681 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
    28741 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
    28801 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
    28861 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
    28921 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
    28981 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
    29041 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
    29101 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
    29161 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
    29221 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
    29281 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
    29341 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
    29401 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
    29461 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
    29521 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
    29581 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
    29641 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
    29701 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
    29761 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
    29821 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
    29881 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
    29941 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
    30001 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
    30061 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
    30121 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
    30181 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
    30241 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
    30301 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
    30361 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
    30421 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
    30481 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
    30541 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
    30601 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
    30661 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
    30721 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
    30781 tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc
    30841 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
    30901 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
    30961 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
    31021 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
    31081 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
    31141 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
    31201 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
    31261 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
    31321 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
    31381 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
    31441 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
    31501 tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa
    31561 agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat
    31621 ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag
    31681 taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc
    31741 ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg
    31801 ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg
    31861 tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc
    31921 ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt
    31981 tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg
    32041 tgttcgatct aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc
    32101 cactacttta ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag
    32161 cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc
    32221 atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc
    32281 ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt tttcttgacc
    32341 atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca
    32401 ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag
    32461 attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa
    32521 gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat
    32581 atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag
    32641 caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca
    32701 cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa
    32761 caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga
    32821 aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa
    32881 gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca
    32941 gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt ctcttacagc
    33001 tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa
    33061 cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa
    33121 attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga
    33181 cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct
    33241 ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat
    33301 aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc
    33361 aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat
    33421 cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa
    33481 ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt
    33541 gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa
    33601 aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc
    33661 ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa
    33721 ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa
    33781 aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg
    33841 taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt
    33901 ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa
    33961 tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa
    34021 tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt
    34081 ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga
    34141 ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg
    34201 ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg
    34261 accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc
    34321 ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt
    34381 cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc
    34441 ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc
    34501 tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg
    34561 ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata
    34621 cattataatg atactgtaaa tatatcttta ttattagtga at
//
LOCUS       DAZKAY010000022        33003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000022 DAZKAY010000000
VERSION     DAZKAY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.022"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1040)
                     /locus_tag="ACG8WP_000954"
     CDS             complement(153..1040)
                     /locus_tag="ACG8WP_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9027278.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1225..2064
                     /locus_tag="ACG8WP_000955"
     CDS             1225..2064
                     /locus_tag="ACG8WP_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9027279.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2260..3234
                     /gene="sgrA"
                     /locus_tag="ACG8WP_000956"
     CDS             2260..3234
                     /gene="sgrA"
                     /locus_tag="ACG8WP_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9027280.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3532..3888
                     /locus_tag="ACG8WP_000957"
     CDS             3532..3888
                     /locus_tag="ACG8WP_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027281.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3933..5048
                     /locus_tag="ACG8WP_000958"
     CDS             3933..5048
                     /locus_tag="ACG8WP_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027282.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5074..5514)
                     /locus_tag="ACG8WP_000959"
     CDS             complement(5074..5514)
                     /locus_tag="ACG8WP_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9027283.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5674..6180
                     /locus_tag="ACG8WP_000960"
     CDS             5674..6180
                     /locus_tag="ACG8WP_000960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9027284.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6348..8261
                     /locus_tag="ACG8WP_000961"
     CDS             6348..8261
                     /locus_tag="ACG8WP_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9027285.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8266..9267
                     /locus_tag="ACG8WP_000962"
     CDS             8266..9267
                     /locus_tag="ACG8WP_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9027286.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9469..9675)
                     /locus_tag="ACG8WP_000963"
     CDS             complement(9469..9675)
                     /locus_tag="ACG8WP_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9027287.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10321..11169)
                     /locus_tag="ACG8WP_000964"
     CDS             complement(10321..11169)
                     /locus_tag="ACG8WP_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9027288.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11333..12235
                     /gene="gnd"
                     /locus_tag="ACG8WP_000965"
     CDS             11333..12235
                     /gene="gnd"
                     /locus_tag="ACG8WP_000965"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9027289.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12237..13238
                     /locus_tag="ACG8WP_000966"
     CDS             12237..13238
                     /locus_tag="ACG8WP_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9027290.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13235..13519
                     /locus_tag="ACG8WP_000967"
     CDS             13235..13519
                     /locus_tag="ACG8WP_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9027291.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13552..14349
                     /locus_tag="ACG8WP_000968"
     CDS             13552..14349
                     /locus_tag="ACG8WP_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9027292.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14363..15196
                     /locus_tag="ACG8WP_000969"
     CDS             14363..15196
                     /locus_tag="ACG8WP_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9027293.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15214..16071
                     /locus_tag="ACG8WP_000970"
     CDS             15214..16071
                     /locus_tag="ACG8WP_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9027294.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16093..16602
                     /locus_tag="ACG8WP_000971"
     CDS             16093..16602
                     /locus_tag="ACG8WP_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9027295.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16639..17040
                     /locus_tag="ACG8WP_000972"
     CDS             16639..17040
                     /locus_tag="ACG8WP_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027296.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17152..17505
                     /locus_tag="ACG8WP_000973"
     CDS             17152..17505
                     /locus_tag="ACG8WP_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027297.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17539..17982
                     /locus_tag="ACG8WP_000974"
     CDS             17539..17982
                     /locus_tag="ACG8WP_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027298.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17999..19084
                     /gene="uxuA"
                     /locus_tag="ACG8WP_000975"
     CDS             17999..19084
                     /gene="uxuA"
                     /locus_tag="ACG8WP_000975"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9027299.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19077..19790
                     /locus_tag="ACG8WP_000976"
     CDS             19077..19790
                     /locus_tag="ACG8WP_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9027300.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(19997..20146)
                     /locus_tag="ACG8WP_000977"
     CDS             complement(19997..20146)
                     /locus_tag="ACG8WP_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027301.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            20542..20742
                     /locus_tag="ACG8WP_000978"
     CDS             20542..20742
                     /locus_tag="ACG8WP_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9027302.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            21310..21873
                     /locus_tag="ACG8WP_000979"
     CDS             21310..21873
                     /locus_tag="ACG8WP_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027303.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(21934..22299)
                     /locus_tag="ACG8WP_000980"
     CDS             complement(21934..22299)
                     /locus_tag="ACG8WP_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9027304.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(22375..22620)
                     /locus_tag="ACG8WP_000981"
     CDS             complement(22375..22620)
                     /locus_tag="ACG8WP_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9027305.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(22690..22944)
                     /locus_tag="ACG8WP_000982"
     CDS             complement(22690..22944)
                     /locus_tag="ACG8WP_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027306.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(23108..23431)
                     /locus_tag="ACG8WP_000983"
     CDS             complement(23108..23431)
                     /locus_tag="ACG8WP_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027307.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            23618..23691
                     /locus_tag="ACG8WP_000984"
     tRNA            23618..23691
                     /locus_tag="ACG8WP_000984"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23652..23654,aa:Ile,seq:cat)
     gene            complement(24076..24573)
                     /locus_tag="ACG8WP_000985"
     CDS             complement(24076..24573)
                     /locus_tag="ACG8WP_000985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9027308.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(24613..25422)
                     /locus_tag="ACG8WP_000986"
     CDS             complement(24613..25422)
                     /locus_tag="ACG8WP_000986"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9027309.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            25756..26613
                     /locus_tag="ACG8WP_000987"
     CDS             25756..26613
                     /locus_tag="ACG8WP_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9027310.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            26606..27508
                     /locus_tag="ACG8WP_000988"
     CDS             26606..27508
                     /locus_tag="ACG8WP_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9027311.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            27541..27891
                     /locus_tag="ACG8WP_000989"
     CDS             27541..27891
                     /locus_tag="ACG8WP_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9027312.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            27940..28863
                     /locus_tag="ACG8WP_000990"
     CDS             27940..28863
                     /locus_tag="ACG8WP_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9027313.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            28941..29834
                     /locus_tag="ACG8WP_000991"
     CDS             28941..29834
                     /locus_tag="ACG8WP_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9027314.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            29872..30435
                     /gene="amaP"
                     /locus_tag="ACG8WP_000992"
     CDS             29872..30435
                     /gene="amaP"
                     /locus_tag="ACG8WP_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9027315.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            30449..30637
                     /locus_tag="ACG8WP_000993"
     CDS             30449..30637
                     /locus_tag="ACG8WP_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9027316.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            30650..31552
                     /locus_tag="ACG8WP_000994"
     CDS             30650..31552
                     /locus_tag="ACG8WP_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9027317.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            31584..31826
                     /locus_tag="ACG8WP_000995"
     CDS             31584..31826
                     /locus_tag="ACG8WP_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9027318.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(32082..32453)
                     /locus_tag="ACG8WP_000996"
     CDS             complement(32082..32453)
                     /locus_tag="ACG8WP_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027319.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        11461 a         5257 c         6626 g         9659 t
ORIGIN      
        1 catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc cccgatgaag
       61 ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc acttctcaaa
      121 ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt tacataagga
      181 tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc catgaattcc
      241 gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta tttgtcttta
      301 ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg ctgataagtc
      361 gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt aaatgctgct
      421 ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt tccatcagtt
      481 tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt ttctccttca
      541 tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat gatctgtgta
      601 ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt taattttaca
      661 ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt atatgtctct
      721 tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt tttgatttgt
      781 tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac atcatcaaat
      841 gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac ttttgggtta
      901 tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt ttcattggac
      961 ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat tagtagttgt
     1021 tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa gaattaggga
     1081 gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa aatctattct
     1141 ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag ggttgttaaa
     1201 cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg atctgtttta
     1261 atagtctttg ttggttttgt tttaggaatt cttgtcaata atgcacttgc ggatatgttt
     1321 attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt agctgttttt
     1381 ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga ttatggtgtt
     1441 tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg aattggagtg
     1501 tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga ttcaggaaac
     1561 gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg ggcatcagaa
     1621 gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc gttaatagca
     1681 atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa catgacattt
     1741 ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat ctacagtaca
     1801 ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca aggagctatt
     1861 ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct gatccaatcg
     1921 caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc aactacatta
     1981 ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa gcggatgaag
     2041 aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc ggtcttgctt
     2101 ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat acacgacatc
     2161 ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt atataattcc
     2221 tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac agcgaccgta
     2281 ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag tacttctgca
     2341 aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat tgtttatgac
     2401 atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa tgctagtgat
     2461 gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa agttattggg
     2521 gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat aatgatttct
     2581 gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat tgttttttat
     2641 gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt tgttggaccg
     2701 ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag cggtgaaagc
     2761 agtacagaga gtacgagcag tgaaattagc acagaaaaca cgagcagcga aagcagtacg
     2821 gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag tacggagagt
     2881 acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg aagcacgagc
     2941 agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat cataccaact
     3001 aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca gcctgatttt
     3061 gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag aaaaagcgat
     3121 ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg tgtgttactc
     3181 attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa ataaagaata
     3241 aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat ttcattcgag
     3301 agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt tactaacata
     3361 tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa taattgacaa
     3421 tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt gtacgttatt
     3481 catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac tttgtttgaa
     3541 gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg ttatgaaaga
     3601 gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac cctggctaag
     3661 atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga atataaatta
     3721 tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt tttactgagt
     3781 aaagtttatt ctatactttt agagggaacg attggttata ttttactgga ttcactttac
     3841 aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt ttgcgtacga
     3901 ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta tcttgcagct
     3961 tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt tttcacttga
     4021 tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata aaaaataaac
     4081 gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata gatagaaaga
     4141 aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac tcgattacgt
     4201 atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa gagtttacgc
     4261 attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac atcactccaa
     4321 cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg gaagaagatt
     4381 tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg tctattgtag
     4441 aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat tatctatatg
     4501 tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag aaggtggatg
     4561 cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg atgtatccaa
     4621 tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat acttattatg
     4681 aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt tttataaaac
     4741 acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag gaattatggg
     4801 caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca tccgctgatc
     4861 agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat gatcaaacga
     4921 atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg cgtctgaaag
     4981 ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa ttggatttcg
     5041 ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa gattattttc
     5101 tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct ttcttttagt
     5161 tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg
     5221 gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa tatatagttc
     5281 actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag aatgagtaga
     5341 gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt catttttggt
     5401 tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga taagccaacg
     5461 ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca tcatacattc
     5521 ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa agtgccattt
     5581 agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat aagaaatact
     5641 attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga cgcaatagta
     5701 gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga attagaaaca
     5761 gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa accagttatt
     5821 ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga gctattgtcg
     5881 gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa ttcattttat
     5941 aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga attatgtggg
     6001 gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt aggataccag
     6061 cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa agcaaaggaa
     6121 acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt ggagcaataa
     6181 gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct gctttattac
     6241 gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat tttttgtata
     6301 aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg aatagcttga
     6361 gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc
     6421 cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg gaggtagaaa
     6481 ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa
     6541 atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg accgctacca
     6601 cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt
     6661 ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt aaaggtgaaa
     6721 atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa atacttaatg
     6781 gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag cttgctgtta
     6841 atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat ctattgtacg
     6901 atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca gatgatggga
     6961 caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat caaactgtac
     7021 taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat caaggttcag
     7081 ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg atcgttacag
     7141 aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat tccagagcga
     7201 ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat gacgtccttt
     7261 actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt tatggtggtt
     7321 cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag
     7381 gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt acgtacagcc
     7441 agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc attggggatc
     7501 aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat caggagatgc
     7561 atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg cctaaattga
     7621 ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt
     7681 tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga aaggcacagg
     7741 aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta gtgttccatg
     7801 atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa gaccaggacg
     7861 aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag acattcgaag
     7921 gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag acgataccaa
     7981 ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa gatccgacag
     8041 agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt gatgatacgc
     8101 aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa agaatgttgc
     8161 cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt ttatctgtta
     8221 gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa atggttcaag
     8281 cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt atttttgatt
     8341 aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga cacaagtgaa
     8401 aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca aatcgatata
     8461 aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat ctacacaccg
     8521 aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc atatgtagga
     8581 ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac gcagcaaatc
     8641 atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc agttatccaa
     8701 ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga ttggtcgaat
     8761 atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt gttagcactt
     8821 caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa caagcagatt
     8881 acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca agtaagtgat
     8941 aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa taaggacttt
     9001 gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg ttggacgcaa
     9061 aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga agaagtaact
     9121 tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga gaaaaatgct
     9181 gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga aacatatcaa
     9241 aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt gtaatctgtc
     9301 acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg tttatgaata
     9361 ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc gaaatttggg
     9421 aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc agatttgttg
     9481 ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa gggacaaaaa
     9541 gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat tgatataagg
     9601 ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa gcaaagaaat
     9661 atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa tagattaaga
     9721 atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag cgaacttgaa
     9781 acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta caaagtttac
     9841 catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag atttaggata
     9901 cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt ttacataaca
     9961 atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc aaaagccagt
    10021 gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa aataagaaac
    10081 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
    10141 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
    10201 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
    10261 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
    10321 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
    10381 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
    10441 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
    10501 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
    10561 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
    10621 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
    10681 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
    10741 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
    10801 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
    10861 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
    10921 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
    10981 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
    11041 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
    11101 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
    11161 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
    11221 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
    11281 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
    11341 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
    11401 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
    11461 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
    11521 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
    11581 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
    11641 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
    11701 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
    11761 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
    11821 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
    11881 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
    11941 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
    12001 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
    12061 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
    12121 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
    12181 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
    12241 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
    12301 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
    12361 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
    12421 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
    12481 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
    12541 tacctgcttt aattgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
    12601 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
    12661 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
    12721 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
    12781 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
    12841 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
    12901 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
    12961 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
    13021 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
    13081 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
    13141 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
    13201 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
    13261 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
    13321 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
    13381 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
    13441 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
    13501 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
    13561 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
    13621 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
    13681 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
    13741 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
    13801 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
    13861 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
    13921 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
    13981 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
    14041 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
    14101 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
    14161 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
    14221 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
    14281 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
    14341 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
    14401 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
    14461 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
    14521 gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc caggaggatc
    14581 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
    14641 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
    14701 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
    14761 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
    14821 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
    14881 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
    14941 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
    15001 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
    15061 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
    15121 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
    15181 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
    15241 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
    15301 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
    15361 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
    15421 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
    15481 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
    15541 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
    15601 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
    15661 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
    15721 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
    15781 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
    15841 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
    15901 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
    15961 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
    16021 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
    16081 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
    16141 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
    16201 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
    16261 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
    16321 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
    16381 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
    16441 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
    16501 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
    16561 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
    16621 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
    16681 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
    16741 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
    16801 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
    16861 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
    16921 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
    16981 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
    17041 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
    17101 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
    17161 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
    17221 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
    17281 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
    17341 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
    17401 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
    17461 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
    17521 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
    17581 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
    17641 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
    17701 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
    17761 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
    17821 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
    17881 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
    17941 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
    18001 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
    18061 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
    18121 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
    18181 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
    18241 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
    18301 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
    18361 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
    18421 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
    18481 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
    18541 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
    18601 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
    18661 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
    18721 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
    18781 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
    18841 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
    18901 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
    18961 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
    19021 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
    19081 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
    19141 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
    19201 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
    19261 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
    19321 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
    19381 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
    19441 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
    19501 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
    19561 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
    19621 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
    19681 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
    19741 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
    19801 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
    19861 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
    19921 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
    19981 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
    20041 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    20101 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    20161 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    20221 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    20281 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    20341 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    20401 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    20461 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    20521 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    20581 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    20641 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    20701 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    20761 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attcattggt cgttgaatgg
    20821 gctgaatagt agtgtataaa acaattttgt ggagcgtgaa gcttttatgg tagaggaaaa
    20881 tacgaataaa gtgatgttga taaaaagatt cctatcttga atatgaatcg agatgtcttg
    20941 aaaatgaatg agtttgacca atcattatcc tatcgaaatt tacagtttcg ttcgattggt
    21001 ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg aagagggcgg cacatctaaa
    21061 tttttaagta acggacagtt caaatatcaa gcattgaaag aggctatcct actgtttgaa
    21121 ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag ttcattaaat cccttcaagt
    21181 gatgaaagaa tgagatctaa taaaacctga tctttcatct gtttttttaa taacgtaata
    21241 atttttttat tttttgtgct ttcgccgtta ttgtagagtg atttagcatt cttattttta
    21301 aaaataactg tgttatatat taaaaaaggc ataatagtta taagcttttt taaagaatat
    21361 ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa gcgttattat tttagtctat
    21421 tttcaggggg ggaaccttat gataaaaaaa ctatttttac tatcaatatt aacgctagca
    21481 atggcaggtt gttctgactc tggcttagca actgaactga tcacaaaatc aactgaagcc
    21541 acgacagagg agttaaaatg tggaaacgat cacagtgact acagaggctt aaatcgacga
    21601 tgcatgggaa tattaggcag aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt
    21661 agaagaagca acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg ctgtgcatcg
    21721 tatacagacg tcgaagatgc ggtactttta aaccacgtca ggagatgttc atttatgtct
    21781 agggaagttg gcgatgaacc aaaactagag tacgctttta gttttgcacg tgaggatgaa
    21841 gataacccta aaacttacga cattttagct tgatctaaga tggttaaagc tttttaatga
    21901 agtaagagga gatagaaggc ttttctagtt atattaggta agataaattc ctttttgctc
    21961 caaatttgct aaacattctg ctagataaac ttcatcgtgt tccggataaa tcggcacgtt
    22021 gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat acataggatt
    22081 tttccactct ggtgctgggc tgtaaccacg tttttccatt tcatccatga caagctgatg
    22141 atattggaat aatctataag gagaatagtg aaaaacataa tccacagtag catgtttctt
    22201 tccccagcca tttcctctta atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg
    22261 aggaagcttt gaaatcaatt tttcatgcca taatcgcata gaaatgtctc ctttatatat
    22321 agattgttaa ccttagacgt taaatgaaaa aaccagcacc aaggctggaa aaaatcactt
    22381 cagtttgctt gctgtttctg agcgaccaat taggtaatca acggaaatat cgtaaaagtc
    22441 agcgatatag attaattcaa tgatgcttgg aatttcttct ccttcttcgt acttacgata
    22501 tttgaataaa ggaatactta acgcgctaga aacagctact tgagataaag atcggccagt
    22561 tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa atagcttctt cgatactcat
    22621 ttaatcacct ttcattatag tagaatagtt ataatgacta tagaaagcta cacatgcaat
    22681 caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga
    22741 caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt
    22801 aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc
    22861 tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa
    22921 caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat
    22981 aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa
    23041 tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt
    23101 gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa
    23161 agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat
    23221 atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca
    23281 tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg
    23341 aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg
    23401 caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt
    23461 ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata
    23521 agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta
    23581 tagttgaaaa gtaacaaaaa taaattttat ttatgatgta cctttagctc agttggttag
    23641 agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtac ataagaggtt
    23701 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
    23761 ttttacttgg ctgttcttat tactgcagat gcaagctttt gaataccgtt taaggaagag
    23821 gttgtgaaat cagcgttttg acctgagtat tagcttaaca aacggaaaaa tagttcctca
    23881 tattttgccg tttgttaagc taataccgag ggtcagctga ttgaacacca tttaagaaag
    23941 aggtcgtgag cctgccgttt cactctgtgt cataaaaaag ccaatctact ttgatagaaa
    24001 aagcacgtag gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa atatcgtaaa
    24061 tgaagataga tatttttatg attcgacgat agagacgttg aaatctttca acagctctaa
    24121 agcggattct ccgtgttttc tactggagac taacattgga tcaacaaaaa agtctgcttc
    24181 aggaaaagca ctctgcaggc ctatcagatt agagagtaca cacaagtttg tttcaatccc
    24241 gactacttcg atctcttttt gttcttcttt gctttcaaat aaagtttcct ggatttcagc
    24301 taaagtttca ggtggaatcg taaagtaata ctttttaatc actcgttcat tttcttgtgg
    24361 agaaagaaga gggatcaatt gcaaatcttc tcgttcttct tctttgtcct tcagttcaac
    24421 tggtatatcc cgtgtgaaga gaatatattc attattttgg cgtgctttag ctaatctttg
    24481 agcaatacgt tctgccaatt gatctgactc tggaatataa aattcgctgt ttggatcaag
    24541 aatatgattt tgcatatcaa taataactaa cattttcttt cctcctacat tcatatcgtc
    24601 attttattct ttttaataga agttttttaa aatataagca acaccagctt cttcattcgt
    24661 cagtgttact tggtttgcaa tttcttttac ttgtcgctgt gcattcccca tcgcaatact
    24721 caacccgctg taattcaaca tagcaatgtc gttttcttga tcgccaaaag ccatgatgtc
    24781 actcgattcg attccaaggt aatccgccag aaaaacaagt gaatgagctt tagtgatacc
    24841 tgtaggcgtg atttctaaga aatgcggatc agagaatact gattggatac catctgcttg
    24901 cagatctctt tgtagcttct gtagtttttc ttcactctta tctgttaatg ctaatttaag
    24961 atagctaaga gaaggatctt ccaacatatt taaaatttga tcaaaagaat ggttctgtaa
    25021 attgaaatgt tcataataag ggtcatcttt gactagattt gattcatcga atgtcagctg
    25081 atcatcttgg gtttctcggc tagcaatgat ggatattttc gcttcttgag catagaagaa
    25141 ggcttttctc agtgtatcgc tatctatttt tgaacgtctg agaactttcg ccatttttcc
    25201 atccttcatt tcagcaatca gtgaaccatt ggaagaaatc aaatagccac cgattcctaa
    25261 ttcggaaaaa atatctttag cagaaatgct gttccttccg gtagcgacgg ttacgattcc
    25321 gccattttca tcaaatttcc gaagtgcttg gatattttct tctggtatct ttccatttcg
    25381 atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc attttatctt cctactttct
    25441 ttttacatat ctattttcct gttaccttaa gattaaatga gaatttaaaa aaatgaaact
    25501 aacttgcttt gtcattatga attttttcaa aaaacattct taagaaaaag gtggctatat
    25561 atagtagttt ttggtcaata cctttgaata aaaagccatg aaaaacgaat agttagcgct
    25621 taccgatgcg ttttgtgata aaaaaatttt aaaatattca atgaaaacaa agcatatatt
    25681 ttgctttaaa accaaatgag gaatattcta gatgtagaac aggttagaaa aagattagaa
    25741 aaaggagtga ttttgatgga gaagagatac ggcgtagttc ctgtggtaac tgcttcagac
    25801 gaaaattatg ctccttactt gagcgtgatg atcgcaactg cattagaaaa ttgcaataaa
    25861 acaagacgca taaaatttta tgtgattgac gatggtctat cagaatatag taaagaagga
    25921 ttggaagaaa cggtaaataa atattcaagt aatgcaagta tccaattcct gacagtggaa
    25981 aaagatatct acgaagactt cctagtcagt gaccatatca caactaccgc ttacttaaga
    26041 atttctttac caaacttatt agctaaagag gattataaga aagtccttta cttagattca
    26101 gatgtgttag tactagacga tatcgtaaaa ctatatgatg agccgttaaa tggaaaaaca
    26161 atcggcgcta ttatcgatcc aggccaagtg aaagctttgg aaagattagg aatcgattcc
    26221 gatgacttgt acttcaattc aggagtgatg gtgattgaca ttgatcaatg gaataaaaaa
    26281 gaaattacag aaaaaactat ccattacctc agtgaaaatg gcgatcgaat catctatcac
    26341 gaccaagatg ctttgaatgc tgtactttat gaagattggg aacaacttca tccaaaatgg
    26401 aacatgcaga cttcgctgat ttttgaacgt catccagcac caaatgaaaa atatgaacgt
    26461 cagtataaag aaggaaatga aaaaccttcg atcgttcact ttacaggaca tgataaacct
    26521 tggaatacgt tgaaagatca tccgtatacc aatttatact taaaaaaatt agcacacagt
    26581 actctaacga aagtaggcga gatcaatgaa taaaaaagaa atagcagtag tcgcaagttg
    26641 taacacaaaa ttcgtaccac atttagctgc attatttgtc tcagtattag ataattgcaa
    26701 cccgtctaaa tttgttcggt tctatgtgat tgacgatgat atcgattttg aaagtaaaca
    26761 gcttttacgc ttctcagtga aaaacgcacg aatgaactca gatgtggaat tcttgaaaat
    26821 caataaagaa ttctttacaa atgtggtaat cagcgatcgt attccagaaa cagcttatta
    26881 tcgtattgcg attccggaat tgttccgcgg cacagaagtt gaacgtatct tatatatgga
    26941 ttgcgacatg atcgctttac aagatatctc taaattatgg cgtcttgact tcggtgattc
    27001 aatcgtagca gctgttgaag acgctgggtt ccatcaacga ctggaaaaaa tgaaaatttc
    27061 agctaaatca atgcgttatt tcaactcagg tttgatgctg atcaatgtga aaaaatggtt
    27121 agatgaaaat attacgcaaa aagtattaga tttcatcgaa cataatccag aaaaattacg
    27181 attccatgac caagatgcat taaatgctat cttacatgac cgttggttac cacttcatcc
    27241 gcgttggaat gcacaaggct atatcatggc aaaagcaaaa aaacatccaa cagcagcagg
    27301 tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt atccattttt caggacatgt
    27361 gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa tattacgaaa aatatgcagg
    27421 gatgacggct ttccgttgtg tagctaaatt ccctaaatat ccaaaatatg caaaagttca
    27481 accaaaaaca gatgtagttg ttaaataata gaagaccaca actgaagagg gggagtagga
    27541 atggaaatta caatgtctaa aaatgcagta gagacgttaa ttgaaaaggt tggggaaaat
    27601 acaaaaatcg cactcgcgct tatcaatgat tccgatccat ttctaaggga caaaggtgct
    27661 tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt cggaatttgg agaatatgcg
    27721 actaaaatcg agcatccatt attggacatc tatacatcta aattagagca aaattatttc
    27781 ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca gcttttcatt agaaaatgaa
    27841 atcgcagtac tcgattataa tatcaaatta aagaactgct tcttcagcta atagtttcga
    27901 gaaagtccaa aaaggaggta tacattgaga tactactttg tggatgaaga aaagaatgta
    27961 atagaaacag atgaaaaaaa atataattgg ctcgtacttg atagtgcaaa gcccgaagag
    28021 attgagcaag caatcgaaga attcgagctt cctgatgata ttttcgtagg aaccgaatat
    28081 ccggaagaag tttctcggat ggagcgcctt tatgagacga aactagagca tccattttct
    28141 ttggtagtga ttaatctaga tcagtcagat aatcgtatcg aaaaaaaatt aacgccaata
    28201 tcttttgtac tttccgataa tctcttgatt acttgtatcg atagaaaaac cgaatttgtg
    28261 gatcggctgc tgacaaagca tggaaatcat ttaaactcgt ttgaaaagat catcacgtat
    28321 acattattag atatttacac ccattacgtg aaagaactaa gagagatgaa aaagcgaatt
    28381 gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg aagaactgta taaacaggca
    28441 gatcttgaaa gagacatcgt atatatcgat cataccttaa gagatcaacg ggaaacaatg
    28501 gatcaattat ggaattctga aactttcatc aaacgtttag acgatgaaca gctactctat
    28561 gatgtccatt tgagacagcg acaaacagaa aaaatgatcg agatctatcg agatttatta
    28621 gagagtattg gtgatctatt caacgggatg atggacaata atctaaatca tatcatgaaa
    28681 tatctagatt cagctgcatt aatcatttcc gttccggcgt tgttcgcagg gatttgggga
    28741 atgaatacag gaggattacc gggtaaaagt tctgctctag gctttatact cgtagtcttt
    28801 ggctctttga tagcagcagt agttttaggt taccatttat accggaaaga ttatacaaat
    28861 taaagaatct atttaaaaca ggtatcaaac aaagaaattt gttttggcaa tacaatttaa
    28921 aaactaggag gaatacagca atgacagaac caaaattaat cgacccgcga aacttgtacc
    28981 atacggagaa atttcctaaa caagatcagg atacaccagc tttacagcag aatatgattc
    29041 ctgttcctga ttgtggagaa gaatcctatg aaggcaataa tcaactagaa aaccgccgtg
    29101 tactaatcac tggtggagat tcagggatcg gtcgtgcagc agcgattgca tttgctagag
    29161 aaggggcaga tatcgctctt caattcttcc caggagaaga aaaagatgca gaggaagtag
    29221 caaaatacgt gaaagatgca ggacgtaaga ttgtcttact accttttgat cttcgtgacg
    29281 aacaagcacc taaagaaatc atagataaag ccgtgagtga acttggcggg ctagatacga
    29341 tggtactaaa cgcaggacag caaatttctt gtcagtctat tgctgagtta ccacttgagc
    29401 aagtcaaaga cacatttatg ataaacatca tcgctatgtt tgctttagtg aaagaagctg
    29461 ttccgcatat cccagcagga ggagcaatcg taacaacaac ttctgttcaa gcattcaatc
    29521 caagcgaaca tttattagat tacgcagcaa caaaagcttc aatcgctaac tttacgatcg
    29581 gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa
    29641 tctggacacc attacaacta gacgaaggac aaccttctga ttcgatccct gaatttggtc
    29701 agcaaagttt actagaacgt gcaggtcagc caggagaatt agcaccagtt tatgttttct
    29761 tagcatcgaa taaagcaagt tatgttacag cccaaattta cggagtaact ggtggagaag
    29821 cgatcaactt ataagaagtt gatcgtttca cccctatgag gaggtgcgaa aatgaataaa
    29881 ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc tatctgtgct tttgtttgcg
    29941 atactattaa gtgcaaccta tccattgcct tttcagctag agaagtttcg attcttttcc
    30001 atcactaatt catacgtgca acaatatgta ttttggcttg ccgttgcatt tgctattttg
    30061 acaattatca cgttacttgt tttcctgttt tatccaaaaa caatcggaaa ctttctactg
    30121 aaacatggtg atggggagtt gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct
    30181 cacttgcatg aagatgaatt catccattca ccaaaagtaa atgtcaaagc aacgaaaaac
    30241 aagatcgccg tcaatgtaaa aggggagttg aaacgaactt cagcactcat tggtaaaaca
    30301 ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca tattgggaac aaaagagcca
    30361 gtgaaagtat cggtgaaata cacaggcttt gaagaaccag ctaaacgaag caatgaccgc
    30421 aaacgagtag aatagggggg gaccaaaaat gaaagaacta ttttcgacat acaagctccc
    30481 tatcatcttt ggggtacttg gattgatatt agccgtttta tggatcaccg tgggcttttt
    30541 caagacctta ttgatcttga tcatggcagc gatcggcgtc gctatcggtt tttatctgaa
    30601 acaaacgaaa atcttagatc actattttaa atcttaggag gaaacatcca tgaataacaa
    30661 taacaaaaat aataacaata aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc
    30721 aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag caccagtatc
    30781 tgcacatcca gatccagtag tccctaacgt tcaacaagga gtagaagaga agaaacatga
    30841 tgcagaaaag catgcacctc atcatccagg aactccagaa tctaaagttc cagaacaaca
    30901 tgatgcaatc gatccttcta aacctgtgga agagcaaaaa catccttctg accatgcgaa
    30961 agcacaagaa aaacttagtt ctgaccacac aaacggacaa ggaaaacttg gttctgatca
    31021 tacaaataac caaggaaagc atgattctga acaggaagat tcaatgcgcg gtgagctgaa
    31081 ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca attgaacaag tagacggttt
    31141 attatctgca aacggtggtt tcttctctaa tgtggcagga aaacttgtga atacagataa
    31201 tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg atatggatgt
    31261 cattgtggaa tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg
    31321 caaagaagta aaaaatatga cacatttaga agtgatcgaa gtcaacgcta acgtagtgga
    31381 catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga
    31441 cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa aatcagcagc
    31501 tggaagcggc gcacaaaaag taaaagaaaa tacagaacca cgggttcagt aataaggaag
    31561 taaaacatta ggaggaacga aacatgggat ttatttggtc acttatcgtc ggaggaatta
    31621 tcggagcaat tgcaggagca atcacgaata aaggttcttc gatggggatt attgcaaatg
    31681 tcgtagcagg tttagttgga tcagcacttg gtcaagcatt attaggttct tggggaccaa
    31741 gtattgcagg aatggcaatc gtgccatcat tgattggtgc catcatttta gtactaatcg
    31801 tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt
    31861 tcttcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
    31921 gggcatatta tcgaagatgc aagcttttga acactgttta tacggaataa gaggctgtga
    31981 caagactttt gtcacagcct cttatttatg cgggcaaatt cccaattaga tttttaaaag
    32041 tttttcctta aatgtgtctt tattgataca gagaagcttc tttaataaaa caatgcctcc
    32101 ctaaattttc ttgagatagg aaaggtgaac atacaaaata atacgaccac tttgaaaacc
    32161 cagatgatcc acaaatggat cgtatgatac tgatgaagta aaaagcaatg gatcagaaac
    32221 tcaaagagat tgaaaaagac aaggaagaaa taatatctct tttttactct tgtctgtttt
    32281 agccaataat agctcgtaaa gtaatctaga caaacaaata gaagtaaaca aagtttcgta
    32341 agagcagcaa tcgtatcatg ttgctctgct agacgattga cgagcaggaa aaatgttggt
    32401 aatggaatat agatcacgag taatgaaaga aaaaagtaaa caataaatgg catactattc
    32461 ctcctaattg tgtgagttcg ccctcatttt aaaataacga tatttgtttg tgaagctttt
    32521 tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa ataatcatta ttatcaaaat
    32581 aatgttaaaa ataaaaaaga attgtccatc actgttattc aaatcatttt atcaaaacta
    32641 catagtaaat gttaaaaagc aacatgacat aatatataga aggctttaca gtatatatgg
    32701 gagcttacta aaaaagaatg gagtaagagt taacacaatg ggccattagc tgaagtttta
    32761 agcacttcaa ggtatgaaac agaatggcct gtaatactac atgattattt agactgctca
    32821 aactgctatc agtctggtgg aatgaataaa gtattatact aaaagcagat ccaactacaa
    32881 cgtaacagga aaccagttgt atcaggaaaa gtgatagtac atggagaaga aaaggccccc
    32941 tttgtatgag tcagtacata tcttatttat agataaaacg gtattcaatc gaaataaaaa
    33001 atc
//
LOCUS       DAZKAY010000023        32577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000023 DAZKAY010000000
VERSION     DAZKAY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.023"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1103
                     /locus_tag="ACG8WP_000997"
     CDS             78..1103
                     /locus_tag="ACG8WP_000997"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9027320.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1142..2092
                     /gene="manA"
                     /locus_tag="ACG8WP_000998"
     CDS             1142..2092
                     /gene="manA"
                     /locus_tag="ACG8WP_000998"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9027321.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            2186..3889
                     /locus_tag="ACG8WP_000999"
     CDS             2186..3889
                     /locus_tag="ACG8WP_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9027322.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3891..5923
                     /locus_tag="ACG8WP_001000"
                     /pseudo
     CDS             3891..5923
                     /locus_tag="ACG8WP_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            5934..6374
                     /locus_tag="ACG8WP_001001"
     CDS             5934..6374
                     /locus_tag="ACG8WP_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027323.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6574..7701)
                     /locus_tag="ACG8WP_001002"
     CDS             complement(6574..7701)
                     /locus_tag="ACG8WP_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027324.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7704..8381)
                     /locus_tag="ACG8WP_001003"
     CDS             complement(7704..8381)
                     /locus_tag="ACG8WP_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9027325.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8645..10411
                     /locus_tag="ACG8WP_001004"
     CDS             8645..10411
                     /locus_tag="ACG8WP_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9027326.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10426..10863
                     /locus_tag="ACG8WP_001005"
     CDS             10426..10863
                     /locus_tag="ACG8WP_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027327.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10876..12027
                     /locus_tag="ACG8WP_001006"
     CDS             10876..12027
                     /locus_tag="ACG8WP_001006"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9027328.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(12089..12787)
                     /locus_tag="ACG8WP_001007"
     CDS             complement(12089..12787)
                     /locus_tag="ACG8WP_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9027329.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(12780..14132)
                     /locus_tag="ACG8WP_001008"
     CDS             complement(12780..14132)
                     /locus_tag="ACG8WP_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9027330.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(14355..15755)
                     /locus_tag="ACG8WP_001009"
     CDS             complement(14355..15755)
                     /locus_tag="ACG8WP_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9027331.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15916..16653
                     /locus_tag="ACG8WP_001010"
     CDS             15916..16653
                     /locus_tag="ACG8WP_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9027332.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            16778..17257
                     /locus_tag="ACG8WP_001011"
     CDS             16778..17257
                     /locus_tag="ACG8WP_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9027333.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            17254..18021
                     /locus_tag="ACG8WP_001012"
     CDS             17254..18021
                     /locus_tag="ACG8WP_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9027334.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            18251..18820
                     /gene="lepB"
                     /locus_tag="ACG8WP_001013"
     CDS             18251..18820
                     /gene="lepB"
                     /locus_tag="ACG8WP_001013"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9027335.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(18924..19526)
                     /locus_tag="ACG8WP_001014"
     CDS             complement(18924..19526)
                     /locus_tag="ACG8WP_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9027336.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            19632..19862
                     /locus_tag="ACG8WP_001015"
     CDS             19632..19862
                     /locus_tag="ACG8WP_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027337.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            19853..20101
                     /locus_tag="ACG8WP_001016"
     CDS             19853..20101
                     /locus_tag="ACG8WP_001016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027338.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            20207..20791
                     /locus_tag="ACG8WP_001017"
     CDS             20207..20791
                     /locus_tag="ACG8WP_001017"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9027339.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            20794..21867
                     /gene="prfA"
                     /locus_tag="ACG8WP_001018"
     CDS             20794..21867
                     /gene="prfA"
                     /locus_tag="ACG8WP_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9027340.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            21860..22699
                     /gene="prmC"
                     /locus_tag="ACG8WP_001019"
     CDS             21860..22699
                     /gene="prmC"
                     /locus_tag="ACG8WP_001019"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9027341.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            22692..22871
                     /locus_tag="ACG8WP_001020"
     CDS             22692..22871
                     /locus_tag="ACG8WP_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027342.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            22893..23918
                     /locus_tag="ACG8WP_001021"
     CDS             22893..23918
                     /locus_tag="ACG8WP_001021"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9027343.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            24093..25337
                     /gene="glyA"
                     /locus_tag="ACG8WP_001022"
     CDS             24093..25337
                     /gene="glyA"
                     /locus_tag="ACG8WP_001022"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9027344.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            25490..26437
                     /locus_tag="ACG8WP_001023"
     CDS             25490..26437
                     /locus_tag="ACG8WP_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9027345.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            26571..27200
                     /gene="upp"
                     /locus_tag="ACG8WP_001024"
     CDS             26571..27200
                     /gene="upp"
                     /locus_tag="ACG8WP_001024"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9027346.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(27339..27581)
                     /locus_tag="ACG8WP_001025"
     CDS             complement(27339..27581)
                     /locus_tag="ACG8WP_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027347.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            27702..27774
                     /locus_tag="ACG8WP_001026"
     tRNA            27702..27774
                     /locus_tag="ACG8WP_001026"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27735..27737,aa:Thr,seq:cgt)
     gene            complement(27893..28531)
                     /locus_tag="ACG8WP_001027"
     CDS             complement(27893..28531)
                     /locus_tag="ACG8WP_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9027348.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(28654..29286)
                     /locus_tag="ACG8WP_001028"
     CDS             complement(28654..29286)
                     /locus_tag="ACG8WP_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9027349.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            29637..29936
                     /locus_tag="ACG8WP_001029"
     CDS             29637..29936
                     /locus_tag="ACG8WP_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9027350.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            30431..>32577
                     /gene="acm"
                     /locus_tag="ACG8WP_001030"
     CDS             30431..>32577
                     /gene="acm"
                     /locus_tag="ACG8WP_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9027351.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFL"
BASE COUNT        11235 a         5644 c         6522 g         9176 t
ORIGIN      
        1 gtaaaaaagg ttgattcctc atagattaag ctttaagctt ggactgtaag aatctgatag
       61 aaaagcagga gaaaaagatg tatccacaca cgaaacagct tattttgaaa aagatggaag
      121 aaggatttta tccaggtgta gcagtcgcct tcataaaaga aaatcaagta gaaacattta
      181 cagccggctt tgcccaagtc gttcctgaaa agcagctgat gacagaagat ctgttgtttg
      241 atgcagcctc actgacgaaa gtcgttgcaa ccacttcatt agttttacgt ctattagaag
      301 atggaaagat agaactcgat caaccgcttc ataaatatct tcctgaattt gaaaatcaga
      361 caatcacttt gcgtcattta ctgacacata cttctgatat caaaacatgg ataccaaaca
      421 gagataagct atctcaggaa gaattgatcg ccgcttattt gaagctagaa ccaggtgagc
      481 aactaggaaa agaagtaaaa tatacagatg ccggtacgat tcttcttggg ttgatgctag
      541 aggaaattta ccaagaaact gcagtggaaa tctttcaaga acaagtattg gaaccattag
      601 gaatgatgaa cagcttattt cttccttttc cttccaaaaa aatcgttccc acagaacaac
      661 tgccttcggg tgaggttttg cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg
      721 aacatgcagg taacgcgggt ctctttattg atttgaaaga cagcattact tttgtccaaa
      781 tgtatttgaa caagggaaaa atccgtaatg gtcgtttttt agaagaagag acgattgatt
      841 ctttactgca agatcaaaca ccgaaaaaaa atggctatcg ttctttagga tgggacttaa
      901 aaaaggatgt agaagacggt catgcgattc tttttcatac tggttataca ggtacgtttt
      961 tcttgattga tataaaaaca caagaagcgt tgatattttt gtcaaatcgc gtacatcctg
     1021 tcgatcatcg tgcggagtat ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag
     1081 agaaagcggt attaccgaaa taatgctata atagctatga aaaatgagag gagtttgaat
     1141 tatgcaggaa cctatgtttt taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg
     1201 tttacgttca gtctttggtt ttgatatccc taacgataaa atcggagaag attgggcgat
     1261 cagtgcgcat cctcatggtg tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt
     1321 agatgaacta tggtcagaac acaaggaatt gtttggccat ccttcagaac cagtgtttcc
     1381 attattgatc aagatcttag acgcagaaga tgatctatct gtacaagtac atccagatga
     1441 tgcctatggg atgaaacatg aaggtgaatt aggaaaaact gaatgctggt atatcatcga
     1501 tgctgaacct ggtgcagaaa tcatctatgg tcatcatgca aagacaagag aagaactagc
     1561 tgagatgatc aaagatgggc gctgggatga ccttttgaag aaagtaccag ttaaaaaagg
     1621 tgatttcttt tatgtaccaa gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt
     1681 agaaacacag caaagttctg acacgactta ccgcgtgtat gactacgaca gaaaagatgc
     1741 gcaaggacaa acaagagagc tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc
     1801 gaaaacacca gaattacaaa tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta
     1861 tttagaaact gagtttttca atgtatatga gtgggatatt aaaggaatca catctttcaa
     1921 aaaacaagcc ccttatacat tgatgacggt aatcgaagga gcaggagacc ttgtcgttga
     1981 cggaaaaaca tatccattag aaaaaggaac aagctgtatt attccaaatg gcgtttctga
     2041 gtgggcggtc caaggtgaac tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga
     2101 tagctgaaag cagcagacgg tttttcgttt gctgcttttt ctacataaaa tccgtccatc
     2161 aataaataga agtctggtga aaaagatgga atacaaatgg aaagcaaaca acagcagtcg
     2221 cgtgtctatg aaacaactga gaaaagctgg acatcactta aatgaaatga tcatgccaaa
     2281 tctaagtctg tttatcgctt ggggaatctt gtcattagca acaaaaggtg tgtctggcga
     2341 tttgcacacc acattagaag aggtatgcca ttggatgatc cagctgcttt tacccatctt
     2401 gatcagttat tcaggcggga aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat
     2461 tgcttcttta gggttgatcg ctcgttccaa tgctccacaa atcgtaggag cgatgattat
     2521 cgggccgctt gccggcatcg tatatgaaca atttgtttct agattccatg tgaaattcaa
     2581 ggctggatat gaaatgcttg tcagcaacct actagtagga ctgactggta gcgttatttg
     2641 tataattgga atcgttttta ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc
     2701 cgttgtgtct tggctagtca caatgaatct gttaccactt gtcagtctga tcttagaacc
     2761 tctaaaagta ctatttttca ataatgcaat caatcatggt gtattgacac ctttaggaat
     2821 cgaattctca caaacagtcg ggtactcttc cttatttttg atggaggcaa atcctggacc
     2881 tggtttaggg atcttacttt ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc
     2941 gagtggagca ttgatgatcc atgcgattgg cgggattcac gaaatctatt ttccatttgt
     3001 cttgctgcga ccgtatctat ttttagctgt aatggctggt ggggcaagcg gcacgttgat
     3061 atttcaatgg ttagatacgg gattagctgc acctgtatcg cctgggtcac tactcgtgat
     3121 ttttgccaat gcgccttatc cacaactagc tggggtaagt atagggatag caatcagtac
     3181 atttgtgacc ttcatttgtg cagcgatact attgaaatta ccaaataaag aaaaagagaa
     3241 caaaacggtc aaagaaaggg gagaaatctt ggaaccatct atcattcatt ccgtcatttt
     3301 tgcttgcgac tcaggtatgg gatcaagtgc tatgggagcc gccctgctga aaaagaaatt
     3361 agcagcagaa aaaatcgatc tttctgtcgg atacagctct atctatgaat tgaataacca
     3421 agtaaatcag ctggttatcg tgcaacatga actagcgggt cttgcaagag aaaaagtacc
     3481 tcaaagtcaa atctatgtac tggatcactt tttggaaaca gatatcttgc tgtcacgaat
     3541 aaaagaagcg atccaatttc caaagcggga agaaaaaaaa gagatagcag atatcacaga
     3601 acaaaaaacg tcgcctgcta atattgtatt tttgtataaa aacaacagac gcggctcaca
     3661 aactatgggc atgacgattt taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat
     3721 ctcaaaagaa tcaatagaaa aaatcaaaat ccaagaagaa acgatttata tcgcacaaag
     3781 agaactagct gtaactgaac agctagaaga aaagatccct cggctagttc tagttgaaca
     3841 ttgggtagct acgaatgaat acgaaaaact cgtgaaggga gaaatataag atgtatttat
     3901 cgcccagaga aaaacaatta ttagcagaat ttctcagtag cccgtcacct gtatctatcc
     3961 aaaaaatgat gaatttattg aaggtcagta aacggactat ttaccgcgaa ttagataatc
     4021 ttgaagtctc attgaaatcg gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg
     4081 tcattgaggc atcagaagaa gtattagagc aattaaaaga aaatacagat gggaaaagta
     4141 atgacttatc cactgctaaa cgacaacacg cgattttagc cgagttgtta ctgacgacag
     4201 agccgttgag tcttgctcat ttcatggaat catatcgcat cagtaatacc actttttatg
     4261 cagatatcaa acagttggaa gaaagtattc gccaactgcc tttggaaatc attcggaacc
     4321 agggatacga gatcgaagga ccggaaaaat accgccggct tctgacagcg aatgtattag
     4381 aattagaaat caatgaatat gaactttttc atagtatttc tttcgatgca tcactgaatt
     4441 atttttttcg attcgttgat ccgcaacatc tatctcttgc acgcaaagta atcggggaag
     4501 aattgatcca aaaaaagacc aatctaagtg atagaaagct tgaacatttg gttttgatgc
     4561 tgacattgac gatggagcgt gtgacgaaaa atcatttact gacaaatgag acatataata
     4621 gtctggcaaa caaagaatta ctacagacag ctaaacgatt gttttcacgg atcgcttctg
     4681 agacaaaaca actttatcca gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact
     4741 tcacgaactc atttgatcag aatttctttg aagagacgtt cgatacacag cttgcttatt
     4801 tagtaaaaca actgattcaa gaagtaagtg aagagacgaa tgtccatttt tatgaagatg
     4861 aaactttgta caaaatgctg ttgactcatt tatctggtgt gttttcaaga gccgttttac
     4921 aagaagagac actgaccaat ccgattttag aaaaaatcat ggttcaatac gaggaagttg
     4981 cgaatgcaat ccgtaaagca gtagagcgga tttttcaaga aaaaaattat ccgaagaaga
     5041 gatcgcttat atggtccttc attttgcgaa ttcattggaa cgaagtccga aagaaagcaa
     5101 tatcagtatt gcaggatttt cgccaagtgg attagcttct actagcatgt tggaactacg
     5161 attgaaaagg tattttccct tcatccaaca aattcatttc tatcggattg ctgatttaaa
     5221 aaaaatcgat ttacaagaag aatatgatgt tgttatttct acatctattt taccaggata
     5281 cggtggaaaa tatattttag tctccccgct actgctagaa gatgaagtca agcagctaaa
     5341 agaagctttt tcgcatattg agaagaaaaa aaggcaaaaa attacgtata acagcaataa
     5401 cgagtggatg atagaagata cttatgagga aacaatgcat ttcatggatc aaatcaatca
     5461 attactcggt cgttttttta tccaagaact ggataatcct gagacgattt ccgaaacgat
     5521 tcgcagctta tttccttatt ttcctgaagg actagtaacg gatgaagagg tggttcatcg
     5581 ccggctgctt aagcgatatg aacaagcacc tatcggtatt cctcatacaa atatggggtt
     5641 attccataca tccagctcat tgatttctaa accgatcttt tgtattttca atttgaaaca
     5701 tccgttgacc attgaaggga tggatagaca gccagtcgat ttgtcccgta tgttagttat
     5761 gctggcgcct tcgccaatcc aagaaaaaga cgggattctt ttaggaaaga tcagtggagc
     5821 aatcatcatg aatgacttga acaccgagat cttccgctca ggaaataaag aaatcgttta
     5881 tcagttgtta gcgaaaatcc tgattgaaga aataaagaaa tgaaggtgaa aagatgatac
     5941 agctaccagc agaaaaaata ttgctcgatc aatcctatca aaattggaaa gaagcgttga
     6001 ccgagatcgg ccagtggatg acccaacaag ggggaatcaa accggcatat atccaatcaa
     6061 tgatcgaaag acaagaaaaa gcgagtgtct atatcgggaa tttcgtagcc ttgccacatg
     6121 ggactgacaa agatgaagaa gtagtagaag aaggaatcta tttagtccaa gtgcctgatg
     6181 gtgtggactt tggaacaaaa gaggaaccaa aaatcgcgac actcttattt gtagtggctt
     6241 taaaaaaaga acatcagtta gcggtgctcc aagatttagc ctttttctgt tcagacatcg
     6301 accaagtaat ggcgctcagt gatgcggaaa ccattgaaga agtccaaatg atttttgaac
     6361 atggacatat ataaccaatg ttcacagtaa aaaaagctgc cttgcatcaa gtaataactt
     6421 caaccaagat aaaaataaga ggagctattt ttgttgggag gttcttatta ctgcggatgc
     6481 aagcttttga aaactattta ttctagaaaa ccgtttattt gaaatagggg acggtgacaa
     6541 ggtttttgtc accgccccta ttatttttgt gttttaatag ctctttttta tatagatagt
     6601 gcccatacct atgaataata aaacgatact ccatatacca agtgtcaaga taccattcat
     6661 gaaagtaacc tgttctgaag ctagtggcga gaaaatatct cctcctatga tcacattttg
     6721 aaaatcaaca taagaagttg gcaagtaatg actgattgat tctatccatc caatcgaaga
     6781 aaacaagcga tactctgcta agaaaagaca aagaagtgta cctaaaagat tgactagaga
     6841 atgttcaaat aaagcagata gaagaaaact gattgtactt aaaaagagca cccacaacaa
     6901 caggaacaga aagctctgga aaagatactc atacatcggc aggattcgta tctcctgacc
     6961 aatgatcgtt cctacaggat aacggaagtc tcccaaacca tttttgatta ttactagtat
     7021 agagacgatc aaaaatggca tcacgagact tagcatagct gcaccataat aaatggtaaa
     7081 acggatgcta tgtttcttta ccatactatc cagtaataga ttgactagag agattacctg
     7141 ctttcttttt tcataagaga tgatggaaga aagaaacaag gcggttatac ataaaatcaa
     7201 aaaactagaa atcattcctg aaaaaatatt ttcgtagtaa tttgctaaag agagatgcgc
     7261 tggcaagtca aaaatagaat atctttggat ctgatgttct ttcatataac ggagcaactc
     7321 gacacgtttt tgttgttcaa tgattggaat tccttgtctt tgaccagaaa tcagtcgatc
     7381 cagtatatct ttttcataat gataagttgc ttcgaccatt tgctggacat tccctgtgtt
     7441 ttttgcatgt aagattgctt cgatcagttt gttgcttttc tctaaacttt ccacaaattc
     7501 tttctcattc tcatcttctt tcaacgtttc aatcaaacgt tgcatctctt catagttatg
     7561 attcagttca tcgaattctg tataagcaag tttatgatca aaaaaaagtg ggaatgcaaa
     7621 gagcacgacc aacaatagag gaaataaaaa gtttactttg ttttttagta agagattgcg
     7681 ttcaaatgta agtaaagaca tagttacacc tcatataatt tacgataaat agctaaaatc
     7741 tcttccatat ttgaggattc tgtgacgatt tctccatttt ttagaaaaca tgctttagaa
     7801 caaatcgatt ggatattcgc gatagcgtgg gaagaaagaa gaatcgtctt cccctctttc
     7861 tgccattgtc tgatgaggct attcaagata tcaatacttc ctgggtctaa tccgttcaac
     7921 ggttcatcga ataacatgac agatgaatca ctgatcaagt aagcagcaag caagacatgt
     7981 tgtttcattc ctagagacat ttttccaatc ggcaaagtaa ggtactcagt catcttcaat
     8041 cgttggaaaa cagtttggca ttctactttg ctctgccaat agtatttgat caaagtcaaa
     8101 taatcaacag ccgatagata gtcggataac tgattcgttg attctagaaa aaagaaagac
     8161 cggcgataat ctgtataagc agtaggagag atgttgtgaa gggacaattg atcataagtt
     8221 gttggcagca gttgggcaat gccattcagc aaagttgttt tgccaaatcc gttcggggca
     8281 atcaaagcag ctacttctcc tgattctact aaaagattca attgtcgaat cacacgtttt
     8341 ttaccacgtt tcatgttcat gtttttgatc agtaatgtca tagataggcc tccaatataa
     8401 aatttaataa cataatagaa tggaaaacat ctaaattaaa tgctaacaaa agttattgtt
     8461 tgcatattat aaaaaaatta aaataacagt tttatttaaa attgaagttt tctgataccg
     8521 aaatcttttt attttgctga catactcaaa cctttggcac aaagtttcgt gccaaagtac
     8581 gactatttct ctttttagta agcggataca attagagtat caaaaggaga gagggagaga
     8641 caaaatggaa cggacgattc ccaaaaacaa catttcgcta aaagcgaggg tacaaaaatt
     8701 aggcagtact ttatcaagca tggtcatgcc aaatattgga gcactgattg cctggggtgt
     8761 attaacggct ctatttatcc cagatggtta tttaccaaat gaggctttcg ctaccatggt
     8821 aagcccaatg ctaacttatc tgattccttt attgatcggt tatactggcg gaaaagtaat
     8881 tgctggtgac cgcggatcag ttgttggggc aattgcaacg atgggtgtga ttgtcggaac
     8941 agatattcca atgatgctag gcgcgatgat catgggacct cttggtggat atgccatcaa
     9001 gaaatttgat cagctattcc aaaaacggat caaatccggt tttgaaatgc tagtcaataa
     9061 tttttcagca ggtcttatcg gttttggatt agcgttatta ggtttttctg cgattggtcc
     9121 agttgtcgat gcattgactc aagcaatggc aaaaggtgta gagatcattt tatctgccca
     9181 tttgattcca ttgacaagta tttttattga accagcaaaa atcttatttt tgaataatgc
     9241 aatcaatcat gggattttga caccattggg aactgaacaa gtagcagagg ctggaaaatc
     9301 tattctattc ttgcttgaag caaatcctgg tccaggactt ggcgtattac ttgcttttac
     9361 tttgtttggc aaaggagcag caaaatcttc agcaccagga gccatgatca ttcacttttt
     9421 aggcgggatc catgaaatct attttcctta cgttatgatg aaaccgttat tattcttatc
     9481 agttataggc ggaggaatgt caggaagttt cattttccaa ttgatggatg ctggactaag
     9541 agcgccagct tctccagggt cgatcattgc gattctagcg atgacaccgt tcaattctta
     9601 ccttcctgtt attttgggcg tgattgttgc cgcagctgtt tcgtttggta tttctgcagt
     9661 aattttgaaa gcggatgcga aagatacaaa tgaagacttc gaaaaatctg ttcaagagac
     9721 aaaggcagct aagcaagctt ccaaaggtgc ccaaggtatt cctcaaggag ctacattctc
     9781 tggcgtacaa aaaatcattt ttgcttgtga tgcaggaatg ggatcaagtg cgatgggcgc
     9841 ttcgatcttg agaaaaaaag tacaggaagc agacttgcct caaacggtga ccaatcaagc
     9901 aatcagccaa ctgacagatg actcgaatac attgatcgtt actcaggtcg aactacaaga
     9961 aagagcaaaa caaaaagcac cgagcgcgcg ttttgtctca gtagaaaact ttttgaattc
    10021 acctcgatat gatgaaatca ttcaagaatt aagcagcaag cagaaaacat cagtttctga
    10081 aattgagaat gagccagcag aagagtttct ttcagcaaac atcaaacaaa tcaaagagat
    10141 tctacttgtc cacgatgatc gttccggatc agcaacaatg gggatgaccg tgctcaaaga
    10201 tatattagac aagaaccatt taactatccc gttaagaaaa gtcaatatcc aagatttaaa
    10261 agatgaacct gattcactga tcgtagccaa agaaagttta gcggaaaaag tacagatggc
    10321 tgcacctcaa tccattcatt tatcagtcaa cagcatggtc aatccgcaaa aatacgaatc
    10381 gattgcacaa tatttgaaac agacagctta agggaggaaa acagcatgca aagtttgcca
    10441 ttcgacatga tcgagctcaa tcgatctttt aaaaccaaag aagaagcgat ccgctattgc
    10501 ggtcagatgc ttgtagaagc agggtgcgtt gatgaggaat atatcgatgc catggtagat
    10561 agagacaaaa tgttatctgt ttatatgggg aattttatcg cgattcctca tggaacagat
    10621 gaagcaaaaa aatatgtgaa aaaatccggc atctgtgtcg ttcaagtacc agatggtgta
    10681 gattttggta ccgaagaaga agaaaagatt gcgactgttt tgttcggtat tgcaggagtc
    10741 ggagatgagc atttgcaact tgtacagcaa atcgcacttt attgcagtga tatggacaat
    10801 gttgtccagc tagcagacgc gctcagcaaa gaagaagtaa cggaaaatct agcaattgct
    10861 taaggagaga gaaaaatgag agcagtacat tttggtgcag gaaatatcgg tcgaggattt
    10921 attggagagg ttttggcagc taatggattt gagattactt tcgtagatgt gaatcacacg
    10981 atcattgatg ctttaaatga aagaaaagaa tacgaaatcg aattagcaga tgaatcaaaa
    11041 gaaaaaatca ctgtaaaaaa tgtcaaagga atcaacaatc aaacaaatcc agaaaaagta
    11101 atccaagaaa tcgctgaagc agatttagtt actaccgcca ttggcccgaa tatcttacct
    11161 tttattgctg agttgattgc aaaaggtatc caagaaagag aaaaggaagg aagcacaaca
    11221 tctttagata tcattgcctg tgaaaatatg attggcggtt cagcatttct agaaaaagaa
    11281 gtgtataaat atcttccgga aacatcattt actgacaaat atatcggctt tccaaatgca
    11341 gcagtcgatc gaatcgtccc attacaacac catgaagatc ctttattcgt ccaagtggaa
    11401 cctttcaaag aatgggtcat tgacgatagc caaagaaaaa ataaaaaaat ccaattaaaa
    11461 ggggttttat atgtagatga tctagaacca tatatcgaac gcaaactatt tagtgtcaat
    11521 actggtcatg cgactgtcgc ttatacaggt gccttgttag gttaccagac cattgatgaa
    11581 gcaatgcaag atgcgttagt tgtggcacaa ttaaaatcag ttttgcagga aacaggaagt
    11641 ttgctgattg ccaaatgggg gtttgatgca gagcagcatc atgcgtatat tgaaaaaatc
    11701 attcatcgct tccaaaacaa aaatatctct gatgcgatca cacgtgtcgc tcggacacca
    11761 ttacgtaagt taggttatca agaaagattt acacgtccag tgagagaact acaagaacat
    11821 aacttgactt gcccacattt gacggcaaca atggggatta tttttaatta ctatgatcct
    11881 gaagatgaac aaagccgtca attgcatgaa atgaagatcc atgaaaactt ggaacaattg
    11941 attcaagaag tcacaggaat caatgatccg aaaacaatcg ggaatatcaa gcaaaacgtg
    12001 aaccgttatg ccaagcaagt cgcttaaaat aggatacgaa aaaggggaat gtgacgaaat
    12061 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
    12121 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
    12181 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
    12241 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
    12301 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
    12361 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
    12421 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
    12481 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
    12541 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
    12601 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
    12661 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
    12721 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
    12781 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
    12841 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
    12901 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
    12961 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
    13021 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
    13081 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
    13141 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
    13201 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
    13261 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
    13321 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
    13381 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
    13441 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
    13501 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
    13561 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
    13621 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
    13681 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
    13741 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
    13801 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
    13861 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
    13921 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
    13981 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
    14041 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
    14101 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
    14161 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
    14221 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
    14281 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
    14341 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
    14401 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
    14461 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
    14521 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
    14581 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
    14641 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
    14701 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
    14761 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
    14821 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
    14881 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
    14941 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
    15001 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
    15061 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
    15121 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
    15181 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
    15241 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
    15301 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
    15361 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
    15421 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
    15481 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
    15541 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
    15601 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
    15661 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
    15721 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
    15781 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
    15841 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
    15901 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
    15961 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
    16021 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
    16081 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
    16141 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
    16201 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
    16261 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
    16321 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
    16381 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
    16441 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
    16501 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
    16561 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
    16621 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
    16681 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
    16741 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
    16801 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
    16861 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
    16921 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
    16981 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
    17041 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
    17101 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
    17161 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
    17221 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
    17281 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
    17341 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
    17401 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
    17461 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
    17521 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
    17581 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
    17641 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
    17701 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
    17761 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
    17821 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
    17881 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
    17941 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
    18001 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
    18061 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
    18121 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
    18181 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
    18241 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
    18301 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
    18361 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
    18421 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
    18481 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
    18541 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
    18601 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
    18661 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
    18721 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
    18781 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
    18841 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
    18901 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
    18961 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
    19021 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
    19081 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
    19141 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
    19201 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
    19261 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
    19321 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
    19381 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
    19441 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
    19501 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
    19561 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
    19621 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
    19681 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
    19741 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
    19801 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
    19861 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
    19921 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
    19981 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
    20041 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
    20101 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
    20161 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
    20221 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
    20281 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
    20341 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
    20401 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
    20461 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
    20521 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
    20581 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
    20641 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
    20701 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
    20761 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
    20821 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
    20881 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
    20941 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
    21001 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
    21061 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
    21121 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
    21181 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
    21241 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
    21301 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
    21361 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
    21421 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
    21481 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
    21541 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
    21601 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
    21661 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
    21721 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
    21781 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
    21841 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
    21901 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
    21961 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
    22021 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    22081 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    22141 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    22201 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    22261 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    22321 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    22381 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    22441 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    22501 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    22561 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    22621 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    22681 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    22741 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    22801 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    22861 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    22921 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    22981 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    23041 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    23101 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    23161 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    23221 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    23281 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    23341 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    23401 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    23461 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    23521 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    23581 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    23641 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    23701 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    23761 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    23821 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    23881 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    23941 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    24001 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    24061 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    24121 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    24181 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    24241 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    24301 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    24361 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    24421 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    24481 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    24541 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    24601 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    24661 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    24721 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    24781 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    24841 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    24901 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    24961 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    25021 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    25081 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    25141 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    25201 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    25261 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    25321 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    25381 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    25441 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    25501 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    25561 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    25621 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    25681 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    25741 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    25801 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    25861 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    25921 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    25981 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    26041 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    26101 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    26161 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    26221 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    26281 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    26341 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    26401 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    26461 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    26521 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    26581 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    26641 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    26701 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    26761 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    26821 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    26881 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    26941 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    27001 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    27061 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    27121 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    27181 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    27241 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    27301 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    27361 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    27421 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    27481 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    27541 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    27601 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    27661 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    27721 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    27781 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    27841 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    27901 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    27961 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    28021 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    28081 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    28141 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    28201 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    28261 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    28321 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    28381 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    28441 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    28501 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    28561 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    28621 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    28681 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    28741 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    28801 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    28861 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    28921 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    28981 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    29041 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    29101 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    29161 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    29221 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    29281 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    29341 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    29401 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    29461 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    29521 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    29581 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    29641 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    29701 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    29761 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    29821 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    29881 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    29941 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    30001 tttatttgaa cagagtctgt atttttttat tctaaataaa gaaacagatg tttctttata
    30061 ttgtatttta tcctttaatt aacacaaaaa cagctagtat aaactaataa tgttaattaa
    30121 aagtgtattt tatgaaacta tcgttattta ttaatgttgc atttttgtaa gttaaaaatt
    30181 ggaaatatag attgaaaaaa tgaaagcaat aagtaaaata aaatgaatat atgcgaaaaa
    30241 ataatttaca ttaggaaaaa ccttgtaaaa acagtttttt ggcatattta ttttttataa
    30301 atatttgtaa aataactatc aattttttag ttaaaagatt ggaaatgagt gggggaggaa
    30361 ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta aaatagggga
    30421 gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta ttattactaa
    30481 tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa
    30541 tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa
    30601 attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg
    30661 gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga
    30721 aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga
    30781 aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa
    30841 cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac
    30901 agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa
    30961 aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa
    31021 taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag
    31081 attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag
    31141 aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc
    31201 agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat
    31261 aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc
    31321 ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac
    31381 agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat
    31441 tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg
    31501 agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg
    31561 agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc
    31621 caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa
    31681 tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc
    31741 aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag
    31801 agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg
    31861 aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac
    31921 gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa
    31981 aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca
    32041 aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa
    32101 tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg
    32161 ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac
    32221 ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta
    32281 tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt
    32341 tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt
    32401 tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc
    32461 taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag
    32521 ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat tcttaca
//
LOCUS       DAZKAY010000024        32225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000024 DAZKAY010000000
VERSION     DAZKAY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.024"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..1039)
                     /locus_tag="ACG8WP_001031"
     CDS             complement(164..1039)
                     /locus_tag="ACG8WP_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9027352.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1148..2917)
                     /gene="aspS"
                     /locus_tag="ACG8WP_001032"
     CDS             complement(1148..2917)
                     /gene="aspS"
                     /locus_tag="ACG8WP_001032"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9027353.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2932..4224)
                     /gene="hisS"
                     /locus_tag="ACG8WP_001033"
     CDS             complement(2932..4224)
                     /gene="hisS"
                     /locus_tag="ACG8WP_001033"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9027354.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4618..5565
                     /locus_tag="ACG8WP_001034"
     CDS             4618..5565
                     /locus_tag="ACG8WP_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9027355.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5654..6913)
                     /locus_tag="ACG8WP_001035"
     CDS             complement(5654..6913)
                     /locus_tag="ACG8WP_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9027356.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7084..7530)
                     /gene="dtd"
                     /locus_tag="ACG8WP_001036"
     CDS             complement(7084..7530)
                     /gene="dtd"
                     /locus_tag="ACG8WP_001036"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9027357.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7541..9754)
                     /locus_tag="ACG8WP_001037"
     CDS             complement(7541..9754)
                     /locus_tag="ACG8WP_001037"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9027358.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9987..10751)
                     /locus_tag="ACG8WP_001038"
     CDS             complement(9987..10751)
                     /locus_tag="ACG8WP_001038"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9027359.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10752..11699)
                     /gene="prmA"
                     /locus_tag="ACG8WP_001039"
     CDS             complement(10752..11699)
                     /gene="prmA"
                     /locus_tag="ACG8WP_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9027360.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11712..12200)
                     /locus_tag="ACG8WP_001040"
     CDS             complement(11712..12200)
                     /locus_tag="ACG8WP_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9027361.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12166..12837)
                     /locus_tag="ACG8WP_001041"
     CDS             complement(12166..12837)
                     /locus_tag="ACG8WP_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9027362.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12975..14261
                     /locus_tag="ACG8WP_001042"
     CDS             12975..14261
                     /locus_tag="ACG8WP_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9027363.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14333..14527
                     /gene="ssrS"
                     /locus_tag="ACG8WP_001043"
     ncRNA           14333..14527
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8WP_001043"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14605..14889
                     /locus_tag="ACG8WP_001044"
     CDS             14605..14889
                     /locus_tag="ACG8WP_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027364.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14962..15441
                     /locus_tag="ACG8WP_001045"
     CDS             14962..15441
                     /locus_tag="ACG8WP_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9027365.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15664..15807
                     /locus_tag="ACG8WP_001046"
     CDS             15664..15807
                     /locus_tag="ACG8WP_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9027366.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16152..16280
                     /locus_tag="ACG8WP_001047"
     CDS             16152..16280
                     /locus_tag="ACG8WP_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027367.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16385..17968
                     /gene="abc-f"
                     /locus_tag="ACG8WP_001048"
     CDS             16385..17968
                     /gene="abc-f"
                     /locus_tag="ACG8WP_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9027368.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18405..19589)
                     /locus_tag="ACG8WP_001049"
     CDS             complement(18405..19589)
                     /locus_tag="ACG8WP_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9027369.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19614..20621)
                     /locus_tag="ACG8WP_001050"
     CDS             complement(19614..20621)
                     /locus_tag="ACG8WP_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9027370.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20793..21152)
                     /gene="comGG"
                     /locus_tag="ACG8WP_001051"
     CDS             complement(20793..21152)
                     /gene="comGG"
                     /locus_tag="ACG8WP_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9027371.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21124..21549)
                     /gene="comGF"
                     /locus_tag="ACG8WP_001052"
     CDS             complement(21124..21549)
                     /gene="comGF"
                     /locus_tag="ACG8WP_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9027372.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21512..21859)
                     /locus_tag="ACG8WP_001053"
     CDS             complement(21512..21859)
                     /locus_tag="ACG8WP_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027373.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21831..22301)
                     /gene="comGD"
                     /locus_tag="ACG8WP_001054"
     CDS             complement(21831..22301)
                     /gene="comGD"
                     /locus_tag="ACG8WP_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9027374.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22276..22554)
                     /gene="comGC"
                     /locus_tag="ACG8WP_001055"
     CDS             complement(22276..22554)
                     /gene="comGC"
                     /locus_tag="ACG8WP_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9027375.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22551..23600)
                     /gene="comGB"
                     /locus_tag="ACG8WP_001056"
     CDS             complement(22551..23600)
                     /gene="comGB"
                     /locus_tag="ACG8WP_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9027376.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23557..24486)
                     /gene="comGA"
                     /locus_tag="ACG8WP_001057"
     CDS             complement(23557..24486)
                     /gene="comGA"
                     /locus_tag="ACG8WP_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9027377.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24618..26372
                     /gene="ade"
                     /locus_tag="ACG8WP_001058"
     CDS             24618..26372
                     /gene="ade"
                     /locus_tag="ACG8WP_001058"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9027378.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26470..27132)
                     /gene="trmB"
                     /locus_tag="ACG8WP_001059"
     CDS             complement(26470..27132)
                     /gene="trmB"
                     /locus_tag="ACG8WP_001059"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9027379.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27202..27984)
                     /locus_tag="ACG8WP_001060"
     CDS             complement(27202..27984)
                     /locus_tag="ACG8WP_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9027380.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28188..29399)
                     /locus_tag="ACG8WP_001061"
     CDS             complement(28188..29399)
                     /locus_tag="ACG8WP_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027381.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29392..30129)
                     /locus_tag="ACG8WP_001062"
     CDS             complement(29392..30129)
                     /locus_tag="ACG8WP_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027382.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30267..30701
                     /locus_tag="ACG8WP_001063"
     CDS             30267..30701
                     /locus_tag="ACG8WP_001063"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9027383.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30694..31038
                     /locus_tag="ACG8WP_001064"
     CDS             30694..31038
                     /locus_tag="ACG8WP_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9027384.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31218..>32225
                     /locus_tag="ACG8WP_001065"
     CDS             31218..>32225
                     /locus_tag="ACG8WP_001065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9027385.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         9643 a         6752 c         5743 g        10087 t
ORIGIN      
        1 catcccataa atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata
       61 aaaaagcagt ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac
      121 gtctgtctac agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat
      181 agaaacgacc aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag
      241 ctttcattgc ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg
      301 tctgcttatc atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca
      361 tacagttttg gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca
      421 tcttcttttg tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg
      481 tactgtaaaa catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat
      541 tgaagtagat cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca
      601 cgatatctag accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca
      661 tcacagcccc cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta
      721 aaatatggat acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct
      781 tcccgatttt taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg
      841 ataccggtac ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac
      901 cagtaatacc acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg
      961 cgatcaacgc ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat
     1021 tttcgaatgt ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt
     1081 gtttttcaat taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt
     1141 ttatcggcta ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg
     1201 aaaccatact tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa
     1261 tgacttcacg aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg
     1321 caataccgcc atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt
     1381 cttccatttg ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag
     1441 tatgaatacg gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt
     1501 ctgcatagac ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg
     1561 tgaatggatg gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc
     1621 aatcagttac ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta
     1681 aacgtgtccg gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca
     1741 tcagcaagtc acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca
     1801 tgaatttagc aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct
     1861 tagctccgaa ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat
     1921 ctgcagcgcc tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga
     1981 agactttgaa ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac
     2041 gtgtatccgg tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa
     2101 atggcaatgt gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg
     2161 tgtaagtttg gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt
     2221 caggctgacg gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac
     2281 ggtcgaatcc agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat
     2341 agaaatgtcc agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag
     2401 atttccctag gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa
     2461 tcgcttttgt tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat
     2521 ccaaataacg atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa
     2581 atggcggggt ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac
     2641 cagtggccat tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt
     2701 cgatcacgta ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag
     2761 gattaaagac gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc
     2821 caaggtctcg tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt
     2881 cggaaactaa tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa
     2941 taaagtcatt tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact
     3001 tttttctttt cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc
     3061 accgatcacc aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc
     3121 tttacggttc atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac
     3181 tttcaatgct tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt
     3241 aaatgcaggg ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat
     3301 tgcaaatccg aagccaggtg ttgaaggacc acctagttct tccactagat tgtcataacg
     3361 tccgcctgcg caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa
     3421 aatcgtatgt gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac
     3481 agccaatgca tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag
     3541 ataatctaag atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc
     3601 caatacgcgt aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc
     3661 aaaaggtaaa agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc
     3721 taatgaattg atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc
     3781 catcaccatg ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat
     3841 ttgatggaat tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat
     3901 ataaaaagtt ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga
     3961 acgaacgatt ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata
     4021 aaaatcatac atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac
     4081 ttcatagtgt tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa
     4141 acgtgctgtt tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt
     4201 tccttttggt ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa
     4261 aatcaccctt gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt
     4321 ttgaaagatt tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg
     4381 tccatctcca gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc
     4441 ctctcttgta aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata
     4501 acctactaaa aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg
     4561 ttttagttca gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg
     4621 gcagaaataa gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat
     4681 gccgttggag gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt
     4741 cgtacagctt tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc
     4801 cgttccgaag aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc
     4861 gtcatcgcta caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct
     4921 gagtttctca aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac
     4981 attttttata tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg
     5041 cacgaattaa aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa
     5101 caattgaaag aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg
     5161 atccatcggc aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt
     5221 gttccctatt ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa
     5281 aaacaatttg gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc
     5341 agagaaattt taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact
     5401 cctgcgcaac ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct
     5461 ggagctaagc gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc
     5521 aatgaagatt atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc
     5581 aatttatagt aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata
     5641 agtttatgct aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact
     5701 gattgaatgg aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct
     5761 gatcggaaaa cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt
     5821 gtcgaaaatt tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt
     5881 ttgctttccc tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat
     5941 catggtctgt tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat
     6001 ctacttctac ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc
     6061 tcatttcttg aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca
     6121 aatctggtct actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg
     6181 ttggacggat gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat
     6241 ctaaatctgt ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat
     6301 tttgtcgagc ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga
     6361 caccttttat gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt
     6421 ctgctaattt ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc
     6481 aaatagatgt aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg
     6541 cttcaagtgc ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg
     6601 gtgtcattag atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct
     6661 gtactccacc ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt
     6721 tgaaattcct tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt
     6781 ctttattgaa aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca
     6841 acatgttgta tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg
     6901 gtacttcttt catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac
     6961 tttttttccg actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta
     7021 gagtttatca gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt
     7081 actttatttg tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac
     7141 cgacatatct gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc
     7201 attgaatgat tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg
     7261 tcgatttcct tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc
     7321 tcccacatct tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg
     7381 tgcaattttt tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa
     7441 taaaacaagt aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc
     7501 tttacttacc cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta
     7561 cactgtagac atcaggtact gctttgattt tatccacgat actctttagg tgggataaat
     7621 tctggatacc aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa
     7681 cgctgttgag atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg
     7741 aacgattata gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat
     7801 cttcccattc gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg
     7861 gacaatctcg acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat
     7921 caccaggaat cggattacag cagcgactga tacgaattag taagttttct accccttgaa
     7981 tgacaacgcc accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca
     8041 tgatttcttc tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag
     8101 tcagacgatt ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat
     8161 catcttctgt ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa
     8221 tatctttagg cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga
     8281 tcacattttc ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc
     8341 tagtagcaac taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga
     8401 tctcaatgat atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt
     8461 ttgcaccagt ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc
     8521 cggatccttt tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac
     8581 taaagatatc tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa
     8641 gttcgagtat ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt
     8701 cattttttcc ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca
     8761 tttcctgtgt tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat
     8821 gcaaagattg gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca
     8881 ttggtttcca ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta
     8941 cgatcactcg aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca
     9001 ttttacgata gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca
     9061 gctcttctgt cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt
     9121 ctctcttcgt ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa
     9181 gtgctgtatc ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg
     9241 catagatttc taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca
     9301 acgtacgcat gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag
     9361 ccattgctag cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt
     9421 ttattttccc taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt
     9481 ctctcagatc ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag
     9541 ccactgtatg gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa
     9601 taatgtaggg ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc
     9661 atgctttctc aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga
     9721 caccgggtcc cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc
     9781 ggtaaaacgg gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta
     9841 tacgtttttt tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc
     9901 attgtataaa taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt
     9961 cgatactctt ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga
    10021 tagctagcag cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct
    10081 aatccacaaa gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca
    10141 ccttctggtc caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg
    10201 gctgcatttg atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca
    10261 taatcatcta gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg
    10321 atctgccgat gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt
    10381 ttcatacgtt tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa
    10441 gcttgcgctc ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga
    10501 tagccactcg ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt
    10561 tctttcagat aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga
    10621 tcattgaatg ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg
    10681 tgatagtttt ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa
    10741 taccgctgca tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc
    10801 tggaaaattt gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc
    10861 ttctcttcaa tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca
    10921 tctgggatca tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca
    10981 atctcgattc ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc
    11041 atatccatat tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca
    11101 tcttttgcac caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct
    11161 agcaatgttt caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg
    11221 ggatgagtgc ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga
    11281 tcttctgtct ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc
    11341 ggatgatagt atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt
    11401 actttatttt ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata
    11461 aaaggcaaga tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct
    11521 tctttcagcg atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa
    11581 tgttcaacat ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata
    11641 ttagcaactg cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata
    11701 tctgtcactc cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt
    11761 acttctacta attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca
    11821 tcgtcttcat ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga
    11881 tattccacta aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt
    11941 actgctagat aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg
    12001 tcttcaaatt cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat
    12061 tgtgctttat tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc
    12121 caatctaaag ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt
    12181 aatagatcct tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt
    12241 tctttgtaaa gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca
    12301 tacacctttg tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt
    12361 tttctcaaaa actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg
    12421 aattgctaat gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc
    12481 gccacgattt ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac
    12541 tccttgagga atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt
    12601 gtaaaggtag atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa
    12661 accatagcta tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata
    12721 tccagctaat cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc
    12781 cacttctggc gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt
    12841 tctcacctac tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga
    12901 aggctgttct ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga
    12961 aggaggaaaa tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg
    13021 aagtcgttgg tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg
    13081 ctcgcatgct ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg
    13141 caagtgcgat cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt
    13201 caaaaaagga tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct
    13261 tattggatga agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag
    13321 aaagtggccg aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc
    13381 cagcgatccg cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc
    13441 agttggcagt cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc
    13501 agttagacga agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg
    13561 cattgaatgg gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc
    13621 atcttacact gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa
    13681 atggagatgc tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg
    13741 tggatgctgc acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt
    13801 gccggcgtct gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg
    13861 ctcgtacagt gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc
    13921 cactagcaga cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg
    13981 cattagatgc agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt
    14041 tgagagatag ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc
    14101 ctcatcattt tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg
    14161 ctcagtatta tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt
    14221 accgtataaa agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt
    14281 tctgcgagct ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct
    14341 gtgatgttcg aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac
    14401 ccgtcttgtg tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat
    14461 gggacccacc tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg
    14521 agattaccat cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg
    14581 ttgcttattt tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta
    14641 ttattattcg ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac
    14701 aaaaatgata aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc
    14761 gtaatgggta ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg
    14821 atcgtaatta ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca
    14881 aagcagtaaa taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat
    14941 tgacttgagg agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat
    15001 ggttctgatg aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac
    15061 ggagaacttc tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt
    15121 gatggaatgc ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa
    15181 agcaatgcga agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca
    15241 aaacaaatta ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga
    15301 gccacaggat tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt
    15361 agaaacgcag cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct
    15421 aaagaccaca ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc
    15481 acaaaaaacc gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt
    15541 aaaataaagg gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc
    15601 gattagtcgc cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc
    15661 aatgtggaag tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc
    15721 gattttatcg ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct
    15781 acattcggta aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac
    15841 gatactcctg aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg
    15901 tattaagcaa atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga
    15961 tttcgcctga acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct
    16021 cagtcagata aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc
    16081 agcgctagtt gaactttctg acactttcag gtacactgga aaagacaact acacgaagga
    16141 gggaaaatac tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc
    16201 atatcgcatt tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca
    16261 aaacagttga attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct
    16321 gagtttaaat aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga
    16381 gatcatgatc atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga
    16441 ggatttatcc tgtcaaattg aagctggtga aaaaattgtc ttagtcggta tcaacggaac
    16501 tggcaaatca acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat
    16561 cagtcgcaaa aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc
    16621 gaccgtcaac cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat
    16681 gagtcgctac gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg
    16741 ttacggtgag ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat
    16801 tgcagcgatc ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact
    16861 tagcggaggc gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt
    16921 acttttactc gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa
    16981 ctatttacag aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga
    17041 tcaagtcacc acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa
    17101 ctacagccgt tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga
    17161 actgcagcaa aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc
    17221 acatgaagga gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc
    17281 gaaactgaca ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa
    17341 tggtggaaaa tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga
    17401 tcaagtacta ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat
    17461 cggcgacaat ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc
    17521 ttacggtggc accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct
    17581 gcagtttgcc gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga
    17641 agaagtcgct cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg
    17701 gatcaaagat ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga
    17761 aaaaatcaac gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga
    17821 agaaattgaa gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg
    17881 ctactttttg cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg
    17941 ccagccgctc ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc
    18001 atacctgtgc tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg
    18061 atcaatgaat gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca
    18121 gcccacgagg ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag
    18181 atgaatgaaa ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt
    18241 tcccttttga ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga
    18301 aattgaagtt aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc
    18361 cctcttgcat gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc
    18421 tccacatcac gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt
    18481 gattcttttg tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt
    18541 tcacaaccaa accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg
    18601 ccgataccag cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg
    18661 atgtattttc ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca
    18721 tagttctttt caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat
    18781 ttcttgttca aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat
    18841 gggagaacag ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt
    18901 gtgaatccca tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca
    18961 tttcctaagt ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc
    19021 gcacgttccg acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa
    19081 tcttcataat aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg
    19141 aaagatgtat cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg
    19201 atccccatta agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg
    19261 tccattactt tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca
    19321 cggtgcccta ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga
    19381 tctaacagca tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt
    19441 tgattatcgc cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc
    19501 cctttcgcaa ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc
    19561 ccagcattga ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga
    19621 gcagatgatt tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt
    19681 aatgaagcaa gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc
    19741 tgtaagatca aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga
    19801 atcattcctt gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc
    19861 gtttctaaaa atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc
    19921 attgattgct ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa
    19981 aactcttttg ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa
    20041 tcgacaggat ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct
    20101 tgtgtcaaat cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca
    20161 attccttctg tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg
    20221 cccattcctg ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt
    20281 tcaataagaa aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc
    20341 aaatgttctg tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct
    20401 ggctcgaaag aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct
    20461 tttgtcggca ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt
    20521 tcgacatagg cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc
    20581 aggttttgat taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga
    20641 tatattataa caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc
    20701 gttttcactg ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata
    20761 acagaaaaga gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt
    20821 tttacttctt ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac
    20881 tgttgtaccc actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt
    20941 tcggcaagcg tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa
    21001 tttttgattc gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat
    21061 aaaaacgtac ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat
    21121 tttctactga tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac
    21181 ataaaaagaa attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat
    21241 aggttgatgt ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat
    21301 atactgttta tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact
    21361 gctcttgagt tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg
    21421 ctgcaaaaat tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag
    21481 actgctcaat atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg
    21541 ttttttcaat actgatcgct tctttctcca tattgacaaa cactttttcg attactccat
    21601 tattatcgac aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt
    21661 gatacatccg ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc
    21721 cacttttctt ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga
    21781 cagaagcgat cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt
    21841 ctttttgtat agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct
    21901 tcccaataaa attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta
    21961 atagaagcat ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt
    22021 ttatctggat tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct
    22081 gtttggatct gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg
    22141 ccgaaaaatt gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa
    22201 gaaaggatcg ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat
    22261 gctaaaacct taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc
    22321 ttcattcaac aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc
    22381 tttttgtgtg tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct
    22441 ataacgcgac aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat
    22501 tagcatttcg atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa
    22561 acctcctcta tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa
    22621 gccgctacgc ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg
    22681 aaaagctgtt ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt
    22741 gcctctccct gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac
    22801 tgctcagcca atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga
    22861 atcagacttt cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct
    22921 tcatgaaaaa gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc
    22981 ggtccaatca ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc
    23041 agacgatacc ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac
    23101 caatgaaaga cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca
    23161 ataagctgag gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc
    23221 aatagcatcg gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga
    23281 aactgtttgg caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg
    23341 atctgtacca gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc
    23401 ccttccttta gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg
    23461 agtaataaat aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt
    23521 tgtgcaaact ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat
    23581 attttcctga gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat
    23641 aaattgataa ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata
    23701 aatgacacca gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct
    23761 gaccaatgta ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag
    23821 tgctgctctt accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa
    23881 gtctggacga tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg
    23941 attgatctgt agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat
    24001 cacttgcaat ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac
    24061 tggtccgctg aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc
    24121 tcgagaacta aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa
    24181 actctctcgt tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc
    24241 taaagaataa gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc
    24301 caaatactta aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa
    24361 tgttcgtttt tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata
    24421 tacgtcttgg gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat
    24481 ttccattatt ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa
    24541 aatgaattta caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta
    24601 tcttgggagg cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa
    24661 aaaagcctgc tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa
    24721 tcatggaaaa agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag
    24781 gaaaaagcgt acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg
    24841 tgcatatcga aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg
    24901 gagttactac tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc
    24961 ttgagtttat gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt
    25021 cttgtgtacc tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag
    25081 cccctttttt acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag
    25141 tagcaaataa tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa
    25201 aaattgacgg gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag
    25261 gtattcggac ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg
    25321 gtatgtattt aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg
    25381 cgatcacacc tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg
    25441 atttagtaaa agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg
    25501 atccattaca agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa
    25561 cactaggtgc gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca
    25621 agctgccaat tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc
    25681 aagaccattt atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg
    25741 ctcaaccatt aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga
    25801 tcgaaattat cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag
    25861 gcgaacgatt cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc
    25921 accatcaaac gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag
    25981 cgatagcgac aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg
    26041 aggatatgct gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca
    26101 acaatcagca ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac
    26161 ctttttcgat cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag
    26221 cagctaaaac ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg
    26281 aattgaagat caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt
    26341 gtggaaaaac cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac
    26401 atctgtaatg ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga
    26461 aagttagttt cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata
    26521 aatacgttga ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa
    26581 ttgactttga tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact
    26641 tgccagagaa tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg
    26701 acgaagaatc tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg
    26761 acgttttttt ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc
    26821 gaaatactct gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg
    26881 ttcgatcaac ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata
    26941 attgatttca ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat
    27001 ctcaatgtgg attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt
    27061 tggatcacta attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt
    27121 tcggacacgc attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct
    27181 ccgcagagtt tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc
    27241 gttcatccgt cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat
    27301 ggccgcatac caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat
    27361 cagccactga ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac
    27421 cgcagggtct gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac
    27481 taaccaatta cgatggttta catctccgtg tactactgat aagttttcct tatctattga
    27541 cgggatctgc tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc
    27601 ttccggtaat tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc
    27661 gatacgggtc agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat
    27721 cacgtcgttt ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg
    27781 tgctgtcaaa gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc
    27841 ttcccttgaa agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc
    27901 atcacgcatg cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc
    27961 ttggtctaac tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta
    28021 cttattattc tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta
    28081 attgccattc aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt
    28141 caacgctcta tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc
    28201 ttcttcaaac gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc
    28261 gctagcggaa cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata
    28321 accgacgcga taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc
    28381 ttatggattg gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac
    28441 agttggaaac cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg
    28501 actactccgg caatcaataa cccacctata attactaatc ggaaataaag accactaaaa
    28561 tcagaacctc ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt
    28621 ttaatatgag tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca
    28681 ggaacatctg tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca
    28741 tgcaccattt tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat
    28801 agacttgcct gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga
    28861 aacaaaaaga tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga
    28921 tctagtcgaa acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat
    28981 atgatcagat agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc
    29041 atggccaatc cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa
    29101 gctcgttcca aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct
    29161 gcctctgcaa aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt
    29221 atgagccctc caattgcaaa cggtggctgc agggttttca accaattaga ataatataat
    29281 cccacaccgc caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc
    29341 atatatttca tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg
    29401 tgcctgctcc tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa
    29461 ttcttttctc aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac
    29521 aaaacgatca caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc
    29581 tccttctttt ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa
    29641 gcctaaaaat ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat
    29701 catcactttc tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga
    29761 taaccgaaaa gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc
    29821 gtaggccatc gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg
    29881 agtctctgga atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag
    29941 atgattgatt tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt
    30001 cgtacttttc cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc
    30061 gaaactgata tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat
    30121 taaactcatt ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac
    30181 ggtattttgt ttttttcctt acaatagctt taacttatgg taaagttaag ctgaagtatt
    30241 tattctttat agaaaggatg ttttatatgg aaaactgtat tttttgcaaa atcatcaatc
    30301 aagaaatccc aagctataaa atatatgaag acgacaaggt ctatgctttt ttagacatct
    30361 ctcaagcaac gaaaggacac acattagttg tgcctaaacg tcatgtagca gatatttttg
    30421 aatatgatcc cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat
    30481 tggaaaaagc ttttccagaa atgaaaggtc ttaatatctt gaataacaac cgtgaactag
    30541 cctatcaatc tgtttttcat tcacatatcc atttagtccc tcgctacagc aaagaggatg
    30601 atttttctat tcactttgtc aatcatcaag atagttatgg atcggaagaa ctaaaagcta
    30661 ttcaagaaac aatcgtaaag caggtgtcct gcgatgatta aaaacttttt caaaggactg
    30721 ttatttggag ctgctgctgg cactgtcgga ggtctgttat tggcgcccag gagcgggaat
    30781 gaaacaagaa agaaactgat cgaagaattg gaagaagcaa caactctgac aaatgattta
    30841 aataacagcc tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac agctgaatct
    30901 gtgattcctg ctttccaaga agcaatcaaa aaagatatcg aagattttaa attccaagcc
    30961 gaaccacgta tcacacaaat caatgaacaa gtagaaaaac taaaagctga tctgccagag
    31021 tatcctgaac aaatataata atgattattt tctcaacaga tttaactata taagtgagaa
    31081 aagtttgcgt atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga
    31141 caacgttttt aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta
    31201 caggagtgca aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg
    31261 ctgttttaac tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag
    31321 gcggaacgat cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat
    31381 ccttagtaca acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa
    31441 ccgataagca agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg
    31501 aatcacaatt agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca
    31561 atttagcttt tgaagctggc ttgaaagctc acgtagatat tacagatgac gatttgaaaa
    31621 ctgcttggaa atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag
    31681 atgaagcaaa agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca
    31741 aagacaaatc aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa
    31801 cgacaacaac tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga
    31861 tctccgatgt tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca
    31921 aaatggtgaa aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag
    31981 atatcgctac tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag
    32041 aattaaaaga tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat
    32101 tcacaacaac ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt
    32161 ctgacacaaa atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat
    32221 caaaa
//
LOCUS       DAZKAY010000025        31896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000025 DAZKAY010000000
VERSION     DAZKAY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.025"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG8WP_001066"
     CDS             complement(26..601)
                     /locus_tag="ACG8WP_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP9027386.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG8WP_001067"
     CDS             complement(606..1580)
                     /locus_tag="ACG8WP_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9027387.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG8WP_001068"
     CDS             1863..2831
                     /locus_tag="ACG8WP_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9027388.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG8WP_001069"
     CDS             2928..3221
                     /locus_tag="ACG8WP_001069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027389.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG8WP_001070"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG8WP_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9027390.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG8WP_001071"
     CDS             3678..4433
                     /locus_tag="ACG8WP_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9027391.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8WP_001072"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8WP_001072"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9027392.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8WP_001073"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8WP_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9027393.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8WP_001074"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8WP_001074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9027394.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8WP_001075"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8WP_001075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9027395.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8WP_001076"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8WP_001076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9027396.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG8WP_001077"
     CDS             9170..9712
                     /locus_tag="ACG8WP_001077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9027397.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8WP_001078"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8WP_001078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9027398.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG8WP_001079"
     CDS             11305..12207
                     /locus_tag="ACG8WP_001079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9027399.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8WP_001080"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8WP_001080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9027400.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLTGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG8WP_001081"
     CDS             13650..14072
                     /locus_tag="ACG8WP_001081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9027401.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG8WP_001082"
     CDS             14541..14771
                     /locus_tag="ACG8WP_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9027402.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG8WP_001083"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG8WP_001083"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9027403.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG8WP_001084"
     CDS             16182..16358
                     /locus_tag="ACG8WP_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9027404.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8WP_001085"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8WP_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9027405.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG8WP_001086"
     CDS             16853..17320
                     /locus_tag="ACG8WP_001086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027406.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG8WP_001087"
     CDS             17405..18289
                     /locus_tag="ACG8WP_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9027407.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG8WP_001088"
     CDS             18403..18639
                     /locus_tag="ACG8WP_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9027408.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG8WP_001089"
     tRNA            18727..18807
                     /locus_tag="ACG8WP_001089"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG8WP_001090"
     tRNA            18814..18885
                     /locus_tag="ACG8WP_001090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG8WP_001091"
     CDS             19011..19181
                     /locus_tag="ACG8WP_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027409.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG8WP_001092"
     CDS             19401..20108
                     /locus_tag="ACG8WP_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9027410.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG8WP_001093"
     CDS             complement(20167..20811)
                     /locus_tag="ACG8WP_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9027411.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG8WP_001094"
     CDS             20938..21225
                     /locus_tag="ACG8WP_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027412.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG8WP_001095"
     CDS             21333..21653
                     /locus_tag="ACG8WP_001095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9027413.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG8WP_001096"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG8WP_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACG8WP_001097"
     CDS             23044..23763
                     /locus_tag="ACG8WP_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9027414.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8WP_001098"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8WP_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9027415.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACG8WP_001099"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACG8WP_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9027416.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACG8WP_001100"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACG8WP_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9027417.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8WP_001101"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8WP_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9027418.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8WP_001102"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8WP_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9027419.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACG8WP_001103"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACG8WP_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9027420.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10608 a         5564 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtctta caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAZKAY010000026        30521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000026 DAZKAY010000000
VERSION     DAZKAY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.026"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8WP_001104"
     CDS             complement(<1..208)
                     /locus_tag="ACG8WP_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027421.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIIS"
     gene            complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACG8WP_001105"
     CDS             complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACG8WP_001105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9027422.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACG8WP_001106"
     CDS             complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACG8WP_001106"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9027423.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1545..2477)
                     /locus_tag="ACG8WP_001107"
     CDS             complement(1545..2477)
                     /locus_tag="ACG8WP_001107"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9027424.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2474..3250)
                     /locus_tag="ACG8WP_001108"
     CDS             complement(2474..3250)
                     /locus_tag="ACG8WP_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9027425.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACG8WP_001109"
     CDS             complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACG8WP_001109"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9027426.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISNKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6494..7570)
                     /locus_tag="ACG8WP_001110"
     CDS             complement(6494..7570)
                     /locus_tag="ACG8WP_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9027427.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7575..8855)
                     /locus_tag="ACG8WP_001111"
     CDS             complement(7575..8855)
                     /locus_tag="ACG8WP_001111"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9027428.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8874..9800)
                     /locus_tag="ACG8WP_001112"
     CDS             complement(8874..9800)
                     /locus_tag="ACG8WP_001112"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9027429.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9813..11096)
                     /locus_tag="ACG8WP_001113"
     CDS             complement(9813..11096)
                     /locus_tag="ACG8WP_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9027430.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACG8WP_001114"
     CDS             complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACG8WP_001114"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9027431.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11884..12795)
                     /locus_tag="ACG8WP_001115"
     CDS             complement(11884..12795)
                     /locus_tag="ACG8WP_001115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9027432.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACG8WP_001116"
     CDS             complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACG8WP_001116"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9027433.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13261..13959)
                     /locus_tag="ACG8WP_001117"
     CDS             complement(13261..13959)
                     /locus_tag="ACG8WP_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9027434.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13946..14332)
                     /locus_tag="ACG8WP_001118"
     CDS             complement(13946..14332)
                     /locus_tag="ACG8WP_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027435.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14479..16149)
                     /locus_tag="ACG8WP_001119"
     CDS             complement(14479..16149)
                     /locus_tag="ACG8WP_001119"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9027436.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16332..16565
                     /locus_tag="ACG8WP_001120"
     CDS             16332..16565
                     /locus_tag="ACG8WP_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9027437.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16664..17095)
                     /locus_tag="ACG8WP_001121"
     CDS             complement(16664..17095)
                     /locus_tag="ACG8WP_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9027438.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17174..17587
                     /locus_tag="ACG8WP_001122"
     CDS             17174..17587
                     /locus_tag="ACG8WP_001122"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9027439.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17625..18773)
                     /locus_tag="ACG8WP_001123"
     CDS             complement(17625..18773)
                     /locus_tag="ACG8WP_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9027440.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18895..19491)
                     /locus_tag="ACG8WP_001124"
     CDS             complement(18895..19491)
                     /locus_tag="ACG8WP_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9027441.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACG8WP_001125"
     CDS             complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACG8WP_001125"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9027442.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22721..23152
                     /locus_tag="ACG8WP_001126"
     CDS             22721..23152
                     /locus_tag="ACG8WP_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027443.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23197..24546)
                     /locus_tag="ACG8WP_001127"
     CDS             complement(23197..24546)
                     /locus_tag="ACG8WP_001127"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9027444.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24616..25779)
                     /locus_tag="ACG8WP_001128"
     CDS             complement(24616..25779)
                     /locus_tag="ACG8WP_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9027445.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25908..26231
                     /locus_tag="ACG8WP_001129"
     CDS             25908..26231
                     /locus_tag="ACG8WP_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027446.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26414..27763)
                     /locus_tag="ACG8WP_001130"
     CDS             complement(26414..27763)
                     /locus_tag="ACG8WP_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9027447.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27832..28785)
                     /locus_tag="ACG8WP_001131"
     CDS             complement(27832..28785)
                     /locus_tag="ACG8WP_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9027448.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28887..30077)
                     /locus_tag="ACG8WP_001132"
     CDS             complement(28887..30077)
                     /locus_tag="ACG8WP_001132"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9027449.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30277..>30521)
                     /locus_tag="ACG8WP_001133"
     CDS             complement(30277..>30521)
                     /locus_tag="ACG8WP_001133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027450.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8578 a         6693 c         5354 g         9896 t
ORIGIN      
        1 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
       61 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
      121 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
      181 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
      241 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
      301 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
      361 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
      421 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
      481 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
      541 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
      601 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
      661 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
      721 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
      781 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
      841 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
      901 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
      961 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     1021 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     1081 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     1141 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     1201 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     1261 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     1321 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     1381 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     1441 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     1501 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     1561 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     1621 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     1681 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     1741 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     1801 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     1861 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     1921 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     1981 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     2041 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     2101 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     2161 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     2221 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     2281 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     2341 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     2401 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     2461 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     2521 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     2581 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     2641 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     2701 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     2761 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     2821 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     2881 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     2941 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     3001 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     3061 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     3121 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     3181 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     3241 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     3301 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     3361 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     3421 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     3481 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     3541 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     3601 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     3661 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     3721 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     3781 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     3841 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     3901 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     3961 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     4021 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     4081 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     4141 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     4201 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     4261 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     4321 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     4381 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     4441 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     4501 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     4561 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     4621 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tattcgaaat
     4681 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     4741 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     4801 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     4861 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     4921 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     4981 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     5041 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     5101 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     5161 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     5221 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     5281 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     5341 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     5401 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     5461 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     5521 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
     5581 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
     5641 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
     5701 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
     5761 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
     5821 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
     5881 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
     5941 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
     6001 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
     6061 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
     6121 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
     6181 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
     6241 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
     6301 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
     6361 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
     6421 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
     6481 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
     6541 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
     6601 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
     6661 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
     6721 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
     6781 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
     6841 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
     6901 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
     6961 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
     7021 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
     7081 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
     7141 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
     7201 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
     7261 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
     7321 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
     7381 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
     7441 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
     7501 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
     7561 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
     7621 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
     7681 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
     7741 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
     7801 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
     7861 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
     7921 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
     7981 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
     8041 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
     8101 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
     8161 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
     8221 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
     8281 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
     8341 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
     8401 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
     8461 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
     8521 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
     8581 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
     8641 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
     8701 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
     8761 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
     8821 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
     8881 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
     8941 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
     9001 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
     9061 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
     9121 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
     9181 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
     9241 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
     9301 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
     9361 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
     9421 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
     9481 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
     9541 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
     9601 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
     9661 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
     9721 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
     9781 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
     9841 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
     9901 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
     9961 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    10021 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    10081 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    10141 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    10201 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    10261 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    10321 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    10381 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    10441 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    10501 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    10561 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    10621 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    10681 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    10741 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    10801 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    10861 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    10921 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    10981 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    11041 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    11101 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    11161 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    11221 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    11281 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    11341 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    11401 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    11461 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    11521 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    11581 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    11641 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    11701 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    11761 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    11821 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    11881 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    11941 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    12001 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    12061 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    12121 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    12181 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    12241 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    12301 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    12361 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    12421 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    12481 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    12541 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    12601 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    12661 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    12721 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    12781 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    12841 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    12901 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    12961 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    13021 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    13081 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    13141 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    13201 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    13261 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    13321 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    13381 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    13441 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    13501 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    13561 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    13621 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    13681 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    13741 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    13801 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    13861 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    13921 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    13981 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    14041 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    14101 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    14161 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    14221 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    14281 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    14341 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    14401 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    14461 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    14521 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    14581 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    14641 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    14701 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    14761 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    14821 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    14881 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    14941 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    15001 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    15061 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    15121 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    15181 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    15241 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    15301 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    15361 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    15421 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    15481 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    15541 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    15601 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    15661 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    15721 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    15781 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    15841 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    15901 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    15961 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    16021 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    16081 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    16141 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    16201 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    16261 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    16321 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    16381 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    16441 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    16501 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    16561 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    16621 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    16681 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    16741 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    16801 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    16861 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    16921 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    16981 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    17041 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    17101 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    17161 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    17221 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    17281 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    17341 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    17401 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    17461 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    17521 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    17581 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    17641 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    17701 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    17761 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    17821 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    17881 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    17941 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    18001 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    18061 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    18121 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    18181 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    18241 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    18301 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    18361 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    18421 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    18481 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    18541 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    18601 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    18661 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    18721 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    18781 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    18841 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    18901 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    18961 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    19021 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    19081 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    19141 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    19201 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    19261 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    19321 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    19381 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    19441 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    19501 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    19561 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    19621 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    19681 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    19741 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    19801 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    19861 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    19921 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    19981 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    20041 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    20101 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    20161 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    20221 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    20281 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    20341 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    20401 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    20461 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    20521 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    20581 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    20641 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    20701 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    20761 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    20821 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    20881 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    20941 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    21001 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    21061 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    21121 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    21181 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    21241 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    21301 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    21361 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    21421 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    21481 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    21541 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    21601 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    21661 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    21721 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    21781 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    21841 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    21901 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    21961 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    22021 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    22081 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    22141 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    22201 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    22261 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    22321 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    22381 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    22441 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    22501 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    22561 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    22621 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    22681 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    22741 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    22801 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    22861 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    22921 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    22981 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    23041 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    23101 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    23161 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    23221 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    23281 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    23341 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    23401 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    23461 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    23521 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    23581 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    23641 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    23701 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    23761 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    23821 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    23881 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    23941 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    24001 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    24061 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    24121 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    24181 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    24241 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    24301 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    24361 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    24421 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    24481 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    24541 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    24601 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    24661 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    24721 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    24781 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    24841 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    24901 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    24961 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    25021 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    25081 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    25141 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    25201 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    25261 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    25321 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    25381 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    25441 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    25501 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    25561 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    25621 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    25681 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    25741 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    25801 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    25861 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    25921 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    25981 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    26041 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    26101 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    26161 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    26221 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    26281 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    26341 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    26401 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    26461 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    26521 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    26581 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    26641 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    26701 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    26761 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    26821 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    26881 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    26941 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    27001 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    27061 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    27121 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    27181 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    27241 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    27301 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    27361 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    27421 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    27481 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    27541 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    27601 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    27661 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    27721 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    27781 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    27841 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    27901 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    27961 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    28021 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    28081 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    28141 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    28201 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    28261 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    28321 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    28381 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    28441 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    28501 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    28561 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    28621 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    28681 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    28741 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    28801 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    28861 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    28921 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    28981 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    29041 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    29101 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    29161 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    29221 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    29281 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    29341 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    29401 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    29461 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    29521 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    29581 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    29641 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    29701 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    29761 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    29821 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    29881 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    29941 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    30001 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    30061 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    30121 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    30181 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    30241 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    30301 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    30361 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    30421 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    30481 ggactgtgac aaaagtcttg tcacagtcct attcttccga a
//
LOCUS       DAZKAY010000027        29337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000027 DAZKAY010000000
VERSION     DAZKAY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.027"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..569)
                     /locus_tag="ACG8WP_001134"
     CDS             complement(135..569)
                     /locus_tag="ACG8WP_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9027451.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(669..1517)
                     /locus_tag="ACG8WP_001135"
     CDS             complement(669..1517)
                     /locus_tag="ACG8WP_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9027452.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1906..3672
                     /locus_tag="ACG8WP_001136"
     CDS             1906..3672
                     /locus_tag="ACG8WP_001136"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9027453.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            3774..5033
                     /locus_tag="ACG8WP_001137"
     CDS             3774..5033
                     /locus_tag="ACG8WP_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9027454.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            5180..6481
                     /locus_tag="ACG8WP_001138"
     CDS             5180..6481
                     /locus_tag="ACG8WP_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027455.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            6484..7335
                     /locus_tag="ACG8WP_001139"
     CDS             6484..7335
                     /locus_tag="ACG8WP_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9027456.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            7348..8163
                     /locus_tag="ACG8WP_001140"
     CDS             7348..8163
                     /locus_tag="ACG8WP_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9027457.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            8314..9480
                     /locus_tag="ACG8WP_001141"
     CDS             8314..9480
                     /locus_tag="ACG8WP_001141"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9027458.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            9710..10357
                     /locus_tag="ACG8WP_001142"
     CDS             9710..10357
                     /locus_tag="ACG8WP_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9027459.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            10441..11385
                     /locus_tag="ACG8WP_001143"
     CDS             10441..11385
                     /locus_tag="ACG8WP_001143"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9027460.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            11520..12971
                     /locus_tag="ACG8WP_001144"
     CDS             11520..12971
                     /locus_tag="ACG8WP_001144"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9027461.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            12992..13828
                     /locus_tag="ACG8WP_001145"
     CDS             12992..13828
                     /locus_tag="ACG8WP_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9027462.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            13828..14487
                     /locus_tag="ACG8WP_001146"
     CDS             13828..14487
                     /locus_tag="ACG8WP_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9027463.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            14613..15302
                     /locus_tag="ACG8WP_001147"
     CDS             14613..15302
                     /locus_tag="ACG8WP_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027464.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            15308..16264
                     /locus_tag="ACG8WP_001148"
     CDS             15308..16264
                     /locus_tag="ACG8WP_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9027465.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            16308..16820
                     /locus_tag="ACG8WP_001149"
     CDS             16308..16820
                     /locus_tag="ACG8WP_001149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027466.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(17076..17624)
                     /locus_tag="ACG8WP_001150"
     CDS             complement(17076..17624)
                     /locus_tag="ACG8WP_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9027467.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(17713..18894)
                     /locus_tag="ACG8WP_001151"
     CDS             complement(17713..18894)
                     /locus_tag="ACG8WP_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9027468.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(19130..20638)
                     /locus_tag="ACG8WP_001152"
     CDS             complement(19130..20638)
                     /locus_tag="ACG8WP_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9027469.1"
                     /translation="MKKNGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            20797..20988
                     /locus_tag="ACG8WP_001153"
     CDS             20797..20988
                     /locus_tag="ACG8WP_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027470.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            21191..22108
                     /locus_tag="ACG8WP_001154"
     CDS             21191..22108
                     /locus_tag="ACG8WP_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9027471.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            22101..23174
                     /locus_tag="ACG8WP_001155"
     CDS             22101..23174
                     /locus_tag="ACG8WP_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027472.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            23322..23840
                     /locus_tag="ACG8WP_001156"
     CDS             23322..23840
                     /locus_tag="ACG8WP_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027473.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            23821..24213
                     /locus_tag="ACG8WP_001157"
     CDS             23821..24213
                     /locus_tag="ACG8WP_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9027474.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            24289..25224
                     /locus_tag="ACG8WP_001158"
     CDS             24289..25224
                     /locus_tag="ACG8WP_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9027475.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            25357..26136
                     /locus_tag="ACG8WP_001159"
     CDS             25357..26136
                     /locus_tag="ACG8WP_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9027476.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            26129..26446
                     /locus_tag="ACG8WP_001160"
     CDS             26129..26446
                     /locus_tag="ACG8WP_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9027477.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            26690..28300
                     /locus_tag="ACG8WP_001161"
     CDS             26690..28300
                     /locus_tag="ACG8WP_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9027478.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            28457..29305
                     /locus_tag="ACG8WP_001162"
     CDS             28457..29305
                     /locus_tag="ACG8WP_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9027479.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         9557 a         5210 c         5800 g         8770 t
ORIGIN      
        1 aatataattg tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg
       61 aaacagacgc gctttctcga atagcaaaca tatacaaaaa atatcagaga caaaaaataa
      121 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaaattact
      181 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
      241 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
      301 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
      361 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
      421 gtaccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
      481 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
      541 acaaaatcga tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
      601 tactagatta ttttattata ccagctaaag aaaacgcatt caaaaaaata gcggatttac
      661 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
      721 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
      781 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
      841 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
      901 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
      961 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
     1021 aaaataaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
     1081 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
     1141 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
     1201 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaacc
     1261 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
     1321 aacataacga tattcaacac taaaataact atagctcggt caaagcctaa agcagcttcc
     1381 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
     1441 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
     1501 tgtctcgttt ctttcattta ttcacttcct taattcatta ataatactct tatcttatta
     1561 taacttactt ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt
     1621 attatgtttc attcttctag aatatacttc tacgccaaaa actgttaaaa aacttttact
     1681 ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa
     1741 gtagactaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa
     1801 ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgcttctcta
     1861 tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca
     1921 atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt
     1981 cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg agatccatat
     2041 accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc
     2101 aatgttcatg attattggga aattgaagtg acgagcgaaa caagtcgctt acagtatgcg
     2161 ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct
     2221 tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa
     2281 atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc
     2341 gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt
     2401 aaagatccag accgagaaga ctttttcggg ggagatttgc aaggtgtgat ggatcatttg
     2461 gattacttga cggaccttgg catcaatggc atctattttt gtcccatatt taaagccaca
     2521 tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa
     2581 aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat
     2641 gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga
     2701 gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag
     2761 atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca
     2821 ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca
     2881 acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc
     2941 gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat
     3001 atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca
     3061 gtgatgaatt atgcatttac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca
     3121 gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac
     3181 gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa
     3241 ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct
     3301 tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa
     3361 tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt
     3421 gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata
     3481 gtaaatgatc gtgaagataa gctctatttc acacggaaac tagagggaca gatcatctat
     3541 gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt
     3601 agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg
     3661 atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt
     3721 gcccattata ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa
     3781 aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag
     3841 cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa
     3901 cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa
     3961 aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat
     4021 tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga
     4081 tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt
     4141 acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag
     4201 taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt
     4261 ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa
     4321 aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag
     4381 gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata
     4441 atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa
     4501 agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata
     4561 aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca
     4621 attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg
     4681 gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc
     4741 ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc
     4801 ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg
     4861 tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt
     4921 ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat
     4981 ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat
     5041 gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg
     5101 agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa
     5161 gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat
     5221 cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg
     5281 tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca
     5341 tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat
     5401 tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt
     5461 gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg
     5521 gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact
     5581 tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac
     5641 tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg
     5701 ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt
     5761 cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat
     5821 tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat
     5881 tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt
     5941 caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt
     6001 tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat
     6061 cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca
     6121 atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg
     6181 cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca
     6241 gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg gcggtcccgg
     6301 aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac
     6361 gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat
     6421 cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta
     6481 acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact
     6541 tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt
     6601 actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact
     6661 ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca
     6721 ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat
     6781 acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt
     6841 cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa
     6901 gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat
     6961 acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa
     7021 cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg
     7081 atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct
     7141 aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt
     7201 tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta
     7261 ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc
     7321 ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca
     7381 ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc
     7441 ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat
     7501 cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa
     7561 ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa
     7621 acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc
     7681 gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa
     7741 tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt
     7801 acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca
     7861 atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt
     7921 ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat
     7981 acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca
     8041 gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata
     8101 actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa
     8161 tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt
     8221 tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa
     8281 cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa
     8341 ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa
     8401 caatacggag atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca
     8461 atgaaagaga ttacaagtaa cgcttctttg gcactaggag aagcctatat ggatcatcga
     8521 atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc
     8581 tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa
     8641 aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg
     8701 ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa
     8761 caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa
     8821 acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat
     8881 aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga
     8941 ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta
     9001 aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa
     9061 aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg
     9121 atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt
     9181 ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat
     9241 ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg
     9301 acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat
     9361 cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat
     9421 gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa
     9481 ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca
     9541 gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt
     9601 atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt
     9661 cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac
     9721 tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa
     9781 tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga
     9841 ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag
     9901 tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata
     9961 cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa
    10021 gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa
    10081 aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa
    10141 agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt
    10201 cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac
    10261 ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca
    10321 aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt
    10381 tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac
    10441 ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct
    10501 ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca
    10561 tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt
    10621 gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat
    10681 ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt
    10741 tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt
    10801 gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa
    10861 ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct
    10921 aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct
    10981 gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt
    11041 ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc
    11101 attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt
    11161 ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt
    11221 gaacatctgc tatgtggcgc aagcatgtta cagattggta cagagcttca taaagaaggg
    11281 ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat
    11341 acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt
    11401 catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg
    11461 tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata
    11521 tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac
    11581 tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg
    11641 cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc
    11701 cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag
    11761 ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc
    11821 ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt
    11881 tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat
    11941 gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt
    12001 tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga
    12061 ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc
    12121 aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt
    12181 atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa
    12241 cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac
    12301 agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag
    12361 ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt
    12421 atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc
    12481 gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga
    12541 agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca
    12601 tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc
    12661 ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg
    12721 attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga
    12781 tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc
    12841 atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag
    12901 atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc
    12961 catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat
    13021 aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa
    13081 cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt
    13141 tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa
    13201 agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca
    13261 aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa
    13321 agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat
    13381 cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc
    13441 agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt
    13501 cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga
    13561 tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc
    13621 tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga
    13681 aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg
    13741 gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga
    13801 aacgatggct gagtcggtga gtgagtgatg tactttcctc taattgattt ttccctcatg
    13861 tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg
    13921 tcgtttcttt taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg
    13981 ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta
    14041 gtcctgctgt ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca
    14101 gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta
    14161 gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg
    14221 gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac
    14281 gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata
    14341 tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta
    14401 gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa
    14461 aaacgttcta tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca
    14521 tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat
    14581 gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag
    14641 aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga
    14701 tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc
    14761 gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac
    14821 tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa
    14881 aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg
    14941 agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg
    15001 taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag
    15061 caaagcaaat ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga
    15121 aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag
    15181 aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg
    15241 attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt
    15301 gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga
    15361 agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga
    15421 ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt
    15481 gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac
    15541 agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga
    15601 agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac
    15661 agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg
    15721 ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa
    15781 gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt
    15841 ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga
    15901 taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa
    15961 tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa
    16021 gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt
    16081 gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact
    16141 gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga
    16201 tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca
    16261 atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc
    16321 aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca
    16381 ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt
    16441 tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa
    16501 cagctgcatt agtctcaggc gtggatcccg tttacacaaa aatagatggt agttggaaag
    16561 aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa
    16621 aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc
    16681 aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg
    16741 ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat
    16801 atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac attaacacta
    16861 ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa
    16921 acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg
    16981 aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgat
    17041 ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc tttttttatt
    17101 ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct
    17161 ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt
    17221 tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata
    17281 aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc
    17341 aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca
    17401 tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg
    17461 aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat
    17521 cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt
    17581 tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg
    17641 atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg
    17701 cttttttgta ttttatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt
    17761 cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa
    17821 tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa
    17881 gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt
    17941 catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc
    18001 ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc
    18061 ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac
    18121 aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc
    18181 tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt
    18241 ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa
    18301 aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga
    18361 catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa
    18421 gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac
    18481 tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc
    18541 tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa
    18601 tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc
    18661 aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa
    18721 tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg
    18781 taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa
    18841 aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc
    18901 actcctattc caaattccgt tttattttac ctgttttgca aataaaatac taatctttag
    18961 tgaaattttc attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg
    19021 aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact
    19081 atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat
    19141 atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg
    19201 tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag
    19261 ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga
    19321 tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc
    19381 cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta
    19441 aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc
    19501 cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag
    19561 gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt
    19621 caggacgtaa accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga
    19681 tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag
    19741 aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt
    19801 cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta
    19861 caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt
    19921 ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt
    19981 tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag
    20041 ctgcatgtcc tttttggctt tcatgataca gttggttcaa gcaagtagcg agctttccac
    20101 ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt
    20161 ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg
    20221 agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag
    20281 gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt
    20341 catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat
    20401 tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt
    20461 gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt
    20521 cgtcaactaa cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca
    20581 aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccattt tttttcacta
    20641 ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag
    20701 agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt
    20761 cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa
    20821 aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat
    20881 cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat
    20941 gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa
    21001 aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc
    21061 tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat
    21121 tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga
    21181 ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga
    21241 gacgaacgcg gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa
    21301 agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg
    21361 cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa
    21421 aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg
    21481 ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta
    21541 ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc
    21601 agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc
    21661 aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga
    21721 aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga
    21781 caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa
    21841 gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac
    21901 aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc
    21961 catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg
    22021 tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc
    22081 taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag
    22141 aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc
    22201 tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacatgaa aaagaaaaag
    22261 taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc
    22321 ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag
    22381 cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac
    22441 aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa
    22501 agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc
    22561 tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata
    22621 cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat
    22681 accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc
    22741 aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag
    22801 ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg
    22861 gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga
    22921 gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc
    22981 catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac
    23041 agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag
    23101 actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg
    23161 tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca
    23221 aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg
    23281 gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag
    23341 cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc
    23401 cgaatttcca gccaatgact gctatttttt tatttatagt actttactta agcttaaaag
    23461 atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac
    23521 catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct
    23581 gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg
    23641 gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac
    23701 cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg
    23761 tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa
    23821 atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt
    23881 tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa
    23941 aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtggtcttc
    24001 aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac
    24061 ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg
    24121 tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga
    24181 caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga
    24241 atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt
    24301 aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc
    24361 agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac
    24421 agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa
    24481 ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc
    24541 gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag
    24601 ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt
    24661 ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat
    24721 actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt
    24781 atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata
    24841 ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc
    24901 ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa
    24961 agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt
    25021 cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa
    25081 gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa
    25141 aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac agataaatat
    25201 gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa
    25261 atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt acaatgagtc
    25321 cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa aagtgcgcca
    25381 tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc agtaggatta
    25441 gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg actgttatcg
    25501 attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac gacgcaagca
    25561 tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac attattagga
    25621 gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa
    25681 tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga cccttcctgg
    25741 aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat
    25801 atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt ccattttgct
    25861 ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact actgatcttt
    25921 acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca aaatactttc
    25981 ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga gaaggcattc
    26041 aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga gccgctcttc
    26101 aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac ttttagttgc
    26161 ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt ttactcatcg
    26221 aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat tatttgcggc
    26281 gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt ggaatatcaa
    26341 aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc
    26401 agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg ttttatttta
    26461 ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact gcatttgcaa
    26521 cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt tggcttatga
    26581 tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg attttttgct
    26641 ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa tgaataaaaa
    26701 attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag ttttaggggt
    26761 agtctatttg attccctggc taagtatgat ggggaacaat caagatggta tgcttgctac
    26821 gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg gtacagtcgg
    26881 atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag ataacgctgc
    26941 ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc
    27001 tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg ctaacgttaa
    27061 taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc caattcttag
    27121 tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat
    27181 tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact tgcgcgtatt
    27241 gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg
    27301 gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct tcgaacttaa
    27361 agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct ctgtctctat
    27421 cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg ttcaaatgat
    27481 cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg cagatcagca
    27541 actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat tgatcctgat
    27601 ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga tgaagaaaaa
    27661 agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt tattgattct
    27721 tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat tgttttttgg
    27781 ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt tgtttttctc
    27841 tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt ttgcgatccg
    27901 attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat taggattagt
    27961 tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg
    28021 ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca gcaatttttt
    28081 cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg ttttcttttt
    28141 gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt gtgtggctgt
    28201 aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat
    28261 gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat cgttttttgc
    28321 tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat tttccaattt
    28381 tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa ttctttgaaa
    28441 cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat atctgccgga
    28501 acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta tggatgggaa
    28561 aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt taaaaaacag
    28621 taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt tatatgagga
    28681 attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag ggatttctgg
    28741 ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg tcattcctta
    28801 tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct tagacttaaa
    28861 tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc gagataatac
    28921 ctatgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac gtttgacaaa
    28981 tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat tttctgaagg
    29041 gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga
    29101 aattattggt agaagaaata acgaaataca accgccagtc aaactttacg tgatacatgc
    29161 aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat atccaaatgc
    29221 agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg aaaaagccat
    29281 cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca aaaatat
//
LOCUS       DAZKAY010000028        29097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000028 DAZKAY010000000
VERSION     DAZKAY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.028"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG8WP_001163"
     CDS             complement(<1..1031)
                     /locus_tag="ACG8WP_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9027480.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG8WP_001164"
     CDS             complement(1113..1802)
                     /locus_tag="ACG8WP_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9027481.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8WP_001165"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8WP_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9027482.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8WP_001166"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8WP_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9027483.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8WP_001167"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8WP_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9027484.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8WP_001168"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8WP_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9027485.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8WP_001169"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8WP_001169"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9027486.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8WP_001170"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8WP_001170"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9027487.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG8WP_001171"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG8WP_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG8WP_001172"
     CDS             complement(7636..8523)
                     /locus_tag="ACG8WP_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9027488.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8WP_001173"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8WP_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9027489.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8WP_001174"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8WP_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9027490.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_001175"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9027491.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8WP_001176"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8WP_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9027492.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG8WP_001177"
     CDS             complement(10454..10957)
                     /locus_tag="ACG8WP_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9027493.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG8WP_001178"
     CDS             11554..12519
                     /locus_tag="ACG8WP_001178"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9027494.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG8WP_001179"
     CDS             complement(12618..13790)
                     /locus_tag="ACG8WP_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027495.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG8WP_001180"
     CDS             complement(13777..14922)
                     /locus_tag="ACG8WP_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027496.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG8WP_001181"
     CDS             complement(14936..15868)
                     /locus_tag="ACG8WP_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027497.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG8WP_001182"
     CDS             15985..17064
                     /locus_tag="ACG8WP_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9027498.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG8WP_001183"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG8WP_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG8WP_001184"
     CDS             complement(17789..18016)
                     /locus_tag="ACG8WP_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9027499.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG8WP_001185"
     CDS             complement(18119..20707)
                     /locus_tag="ACG8WP_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9027500.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG8WP_001186"
     CDS             complement(20727..21386)
                     /locus_tag="ACG8WP_001186"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9027501.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8WP_001187"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8WP_001187"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9027502.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG8WP_001188"
     CDS             complement(22159..23025)
                     /locus_tag="ACG8WP_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9027503.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQLVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG8WP_001189"
     CDS             23134..23775
                     /locus_tag="ACG8WP_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9027504.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8WP_001190"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8WP_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9027505.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG8WP_001191"
     CDS             complement(25216..26115)
                     /locus_tag="ACG8WP_001191"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9027506.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG8WP_001192"
     CDS             complement(26117..26914)
                     /locus_tag="ACG8WP_001192"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9027507.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG8WP_001193"
     CDS             complement(26892..27596)
                     /locus_tag="ACG8WP_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9027508.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG8WP_001194"
     CDS             27727..28302
                     /locus_tag="ACG8WP_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP9027509.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG8WP_001195"
     CDS             28420..29097
                     /locus_tag="ACG8WP_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9027510.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6166 c         5231 g         9137 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgctttaaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacaagt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DAZKAY010000029        27744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000029 DAZKAY010000000
VERSION     DAZKAY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.029"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8WP_001196"
     CDS             275..1753
                     /locus_tag="ACG8WP_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027511.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACG8WP_001197"
     CDS             2030..4090
                     /locus_tag="ACG8WP_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9027512.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACG8WP_001198"
     CDS             4173..4742
                     /locus_tag="ACG8WP_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027513.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACG8WP_001199"
     CDS             complement(4917..5723)
                     /locus_tag="ACG8WP_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027514.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACG8WP_001200"
     CDS             5861..7375
                     /locus_tag="ACG8WP_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9027515.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="ACG8WP_001201"
     CDS             complement(7778..8761)
                     /locus_tag="ACG8WP_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9027516.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="ACG8WP_001202"
     CDS             complement(8766..9413)
                     /locus_tag="ACG8WP_001202"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9027517.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="ACG8WP_001203"
     CDS             complement(9498..10130)
                     /locus_tag="ACG8WP_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9027518.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8WP_001204"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8WP_001204"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9027519.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="ACG8WP_001205"
     CDS             complement(10694..11431)
                     /locus_tag="ACG8WP_001205"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9027520.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="ACG8WP_001206"
     CDS             complement(11428..12417)
                     /locus_tag="ACG8WP_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9027521.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="ACG8WP_001207"
     CDS             12675..13217
                     /locus_tag="ACG8WP_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9027522.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="ACG8WP_001208"
     CDS             13234..14319
                     /locus_tag="ACG8WP_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027523.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="ACG8WP_001209"
     CDS             14331..15137
                     /locus_tag="ACG8WP_001209"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027524.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="ACG8WP_001210"
     CDS             15138..15965
                     /locus_tag="ACG8WP_001210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027525.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="ACG8WP_001211"
     CDS             15962..17035
                     /locus_tag="ACG8WP_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9027526.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="ACG8WP_001212"
     CDS             17463..20159
                     /locus_tag="ACG8WP_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9027527.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20224..20724
                     /locus_tag="ACG8WP_001213"
     CDS             20224..20724
                     /locus_tag="ACG8WP_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9027528.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20736..21230
                     /locus_tag="ACG8WP_001214"
     CDS             20736..21230
                     /locus_tag="ACG8WP_001214"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9027529.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21251..22060
                     /locus_tag="ACG8WP_001215"
     CDS             21251..22060
                     /locus_tag="ACG8WP_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9027530.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22057..22878
                     /locus_tag="ACG8WP_001216"
     CDS             22057..22878
                     /locus_tag="ACG8WP_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9027531.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22929..25028
                     /locus_tag="ACG8WP_001217"
     CDS             22929..25028
                     /locus_tag="ACG8WP_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9027532.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25025..26143
                     /locus_tag="ACG8WP_001218"
     CDS             25025..26143
                     /locus_tag="ACG8WP_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9027533.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26182..27060
                     /locus_tag="ACG8WP_001219"
     CDS             26182..27060
                     /locus_tag="ACG8WP_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9027534.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27206..27403
                     /locus_tag="ACG8WP_001220"
     CDS             27206..27403
                     /locus_tag="ACG8WP_001220"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9027535.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            27387..27617
                     /locus_tag="ACG8WP_001221"
     CDS             27387..27617
                     /locus_tag="ACG8WP_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027536.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8878 a         4742 c         5778 g         8346 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    20221 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    20281 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    20341 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    20401 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    20461 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    20521 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    20581 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    20641 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    20701 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    20761 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    20821 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    20881 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    20941 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    21001 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    21061 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    21121 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    21181 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    21241 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    21301 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    21361 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    21421 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    21481 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    21541 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    21601 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    21661 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    21721 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    21781 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    21841 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    21901 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    21961 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    22021 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    22081 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    22141 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    22201 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    22261 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    22321 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    22381 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    22441 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    22501 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    22561 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    22621 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    22681 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    22741 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    22801 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    22861 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    22921 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    22981 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    23041 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    23101 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    23161 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    23221 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    23281 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    23341 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    23401 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    23461 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    23521 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    23581 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    23641 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    23701 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    23761 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    23821 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    23881 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    23941 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    24001 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    24061 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    24121 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    24181 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    24241 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    24301 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    24361 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    24421 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    24481 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    24541 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    24601 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    24661 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    24721 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    24781 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    24841 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    24901 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    24961 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    25021 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    25081 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    25141 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    25201 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    25261 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    25321 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    25381 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    25441 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    25501 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    25561 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    25621 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    25681 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    25741 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    25801 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    25861 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    25921 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    25981 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    26041 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    26101 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    26161 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    26221 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    26281 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    26341 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    26401 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    26461 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    26521 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    26581 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    26641 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    26701 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    26761 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    26821 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    26881 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    26941 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    27001 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    27061 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    27121 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    27181 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    27241 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    27301 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    27361 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    27421 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa
    27481 aaaagaaagt ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag
    27541 aaattctata tttaccttta actctcgctt ctcttgcaaa tgtagttagc attttaatcg
    27601 ataataaact caggtagtta tattaccttg aaatgattga taattttgtc acttctattt
    27661 tttttgcaat aaatttttta ttttagtgaa aaataagaaa ctatttcttt gaaggcgata
    27721 agttagaaaa atattattgt agtc
//
LOCUS       DAZKAY010000030        27517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000030 DAZKAY010000000
VERSION     DAZKAY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.030"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..966)
                     /locus_tag="ACG8WP_001222"
     CDS             complement(22..966)
                     /locus_tag="ACG8WP_001222"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9027537.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1262..2206)
                     /locus_tag="ACG8WP_001223"
     CDS             complement(1262..2206)
                     /locus_tag="ACG8WP_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9027538.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2427..3641
                     /locus_tag="ACG8WP_001224"
     CDS             2427..3641
                     /locus_tag="ACG8WP_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9027539.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3663..5678
                     /locus_tag="ACG8WP_001225"
     CDS             3663..5678
                     /locus_tag="ACG8WP_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP9027540.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5761..7134)
                     /locus_tag="ACG8WP_001226"
     CDS             complement(5761..7134)
                     /locus_tag="ACG8WP_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9027541.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7160..7444)
                     /locus_tag="ACG8WP_001227"
     CDS             complement(7160..7444)
                     /locus_tag="ACG8WP_001227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9027542.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7446..9461)
                     /locus_tag="ACG8WP_001228"
     CDS             complement(7446..9461)
                     /locus_tag="ACG8WP_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9027543.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9640..9945)
                     /locus_tag="ACG8WP_001229"
     CDS             complement(9640..9945)
                     /locus_tag="ACG8WP_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9027544.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10034..11065)
                     /locus_tag="ACG8WP_001230"
     CDS             complement(10034..11065)
                     /locus_tag="ACG8WP_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9027545.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11272..12147
                     /locus_tag="ACG8WP_001231"
     CDS             11272..12147
                     /locus_tag="ACG8WP_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9027546.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12299..13141)
                     /locus_tag="ACG8WP_001232"
     CDS             complement(12299..13141)
                     /locus_tag="ACG8WP_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9027547.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13233..15110)
                     /locus_tag="ACG8WP_001233"
     CDS             complement(13233..15110)
                     /locus_tag="ACG8WP_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9027548.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15107..16528)
                     /locus_tag="ACG8WP_001234"
     CDS             complement(15107..16528)
                     /locus_tag="ACG8WP_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9027549.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16531..19920)
                     /locus_tag="ACG8WP_001235"
     CDS             complement(16531..19920)
                     /locus_tag="ACG8WP_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9027550.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20177..21568
                     /locus_tag="ACG8WP_001236"
     CDS             20177..21568
                     /locus_tag="ACG8WP_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027551.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21641..22288)
                     /locus_tag="ACG8WP_001237"
     CDS             complement(21641..22288)
                     /locus_tag="ACG8WP_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027552.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22296..22982)
                     /locus_tag="ACG8WP_001238"
     CDS             complement(22296..22982)
                     /locus_tag="ACG8WP_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027553.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22988..24061)
                     /locus_tag="ACG8WP_001239"
     CDS             complement(22988..24061)
                     /locus_tag="ACG8WP_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027554.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24283..24957
                     /locus_tag="ACG8WP_001240"
     CDS             24283..24957
                     /locus_tag="ACG8WP_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9027555.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24962..26302
                     /locus_tag="ACG8WP_001241"
     CDS             24962..26302
                     /locus_tag="ACG8WP_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9027556.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26451..27437)
                     /locus_tag="ACG8WP_001242"
     CDS             complement(26451..27437)
                     /locus_tag="ACG8WP_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9027557.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8008 a         5212 c         5005 g         9292 t
ORIGIN      
        1 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
       61 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      121 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      181 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      241 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      301 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      361 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      421 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      481 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      541 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      601 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      661 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
      721 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
      781 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
      841 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
      901 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
      961 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1021 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1081 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1141 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1201 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1261 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1321 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1381 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1441 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1501 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1561 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1621 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1681 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     1741 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     1801 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     1861 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     1921 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     1981 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2041 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2101 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2161 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
     2221 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
     2281 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
     2341 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
     2401 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
     2461 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
     2521 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
     2581 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
     2641 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
     2701 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
     2761 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
     2821 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
     2881 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
     2941 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
     3001 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
     3061 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
     3121 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
     3181 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
     3241 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
     3301 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
     3361 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
     3421 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3481 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3541 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3601 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3661 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     3721 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     3781 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     3841 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     3901 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     3961 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4021 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4081 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4141 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4201 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4261 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4321 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4381 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4441 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
     4501 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4561 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4621 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4681 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     4741 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     4801 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
     4861 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
     4921 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
     4981 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
     5041 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
     5101 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
     5161 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
     5221 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
     5281 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
     5341 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
     5401 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
     5461 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
     5521 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
     5581 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
     5641 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
     5701 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
     5761 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
     5821 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
     5881 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
     5941 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
     6001 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
     6061 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
     6121 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
     6181 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
     6241 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
     6301 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
     6361 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
     6421 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
     6481 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
     6541 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
     6601 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
     6661 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
     6721 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
     6781 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
     6841 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
     6901 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
     6961 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
     7021 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
     7081 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
     7141 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
     7201 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
     7261 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
     7321 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
     7381 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
     7441 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
     7501 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
     7561 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
     7621 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
     7681 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
     7741 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
     7801 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
     7861 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
     7921 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
     7981 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
     8041 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
     8101 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
     8161 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
     8221 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
     8281 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
     8341 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
     8401 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
     8461 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
     8521 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
     8581 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
     8641 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
     8701 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
     8761 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
     8821 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
     8881 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
     8941 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
     9001 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
     9061 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
     9121 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
     9181 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
     9241 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
     9301 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
     9361 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
     9421 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
     9481 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
     9541 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
     9601 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
     9661 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
     9721 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
     9781 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
     9841 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
     9901 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
     9961 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    10021 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    10081 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    10141 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    10201 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    10261 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    10321 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    10381 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    10441 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    10501 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    10561 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    10621 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    10681 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    10741 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    10801 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    10861 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    10921 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    10981 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    11041 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    11101 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    11161 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    11221 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    11281 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    11341 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    11401 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    11461 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    11521 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    11581 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    11641 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    11701 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    11761 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    11821 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    11881 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    11941 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    12001 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    12061 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    12121 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    12181 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    12241 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    12301 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    12361 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    12421 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    12481 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    12541 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    12601 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    12661 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    12721 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    12781 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    12841 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    12901 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    12961 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    13021 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    13081 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    13141 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    13201 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    13261 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    13321 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    13381 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    13441 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    13501 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    13561 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    13621 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    13681 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    13741 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    13801 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    13861 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    13921 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    13981 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    14041 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    14101 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    14161 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    14221 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    14281 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    14341 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    14401 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    14461 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    14521 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    14581 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    14641 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    14701 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    14761 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    14821 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    14881 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    14941 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    15001 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    15061 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    15121 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    15181 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    15241 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    15301 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    15361 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    15421 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    15481 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    15541 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    15601 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    15661 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    15721 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    15781 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    15841 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    15901 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    15961 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    16021 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    16081 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    16141 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    16201 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    16261 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    16321 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    16381 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    16441 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    16501 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    16561 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    16621 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    16681 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    16741 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    16801 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    16861 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    16921 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    16981 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    17041 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    17101 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    17161 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    17221 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    17281 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    17341 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    17401 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    17461 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    17521 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    17581 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    17641 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    17701 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    17761 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    17821 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    17881 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    17941 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    18001 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    18061 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    18121 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    18181 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    18241 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    18301 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    18361 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    18421 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    18481 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    18541 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    18601 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    18661 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    18721 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    18781 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    18841 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    18901 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    18961 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    19021 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    19081 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    19141 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    19201 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    19261 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    19321 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    19381 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    19441 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    19501 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    19561 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    19621 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    19681 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    19741 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    19801 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    19861 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    19921 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    19981 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    20041 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    20101 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    20161 attaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    20221 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    20281 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    20341 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    20401 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    20461 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    20521 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    20581 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    20641 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    20701 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    20761 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    20821 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    20881 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    20941 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    21001 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    21061 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    21121 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    21181 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    21241 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    21301 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    21361 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    21421 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    21481 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    21541 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    21601 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    21661 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    21721 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    21781 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    21841 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    21901 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    21961 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    22021 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    22081 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    22141 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    22201 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    22261 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    22321 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    22381 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    22441 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    22501 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    22561 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    22621 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    22681 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    22741 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    22801 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    22861 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    22921 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    22981 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    23041 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    23101 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    23161 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    23221 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    23281 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    23341 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    23401 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    23461 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    23521 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    23581 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    23641 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    23701 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    23761 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    23821 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    23881 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    23941 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    24001 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    24061 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    24121 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    24181 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    24241 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    24301 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    24361 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    24421 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    24481 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    24541 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    24601 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    24661 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    24721 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    24781 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    24841 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    24901 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    24961 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    25021 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    25081 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    25141 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    25201 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    25261 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    25321 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    25381 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    25441 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    25501 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    25561 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    25621 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    25681 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    25741 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    25801 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    25861 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    25921 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    25981 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    26041 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    26101 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    26161 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    26221 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    26281 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    26341 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    26401 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    26461 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    26521 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    26581 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    26641 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    26701 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    26761 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    26821 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    26881 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    26941 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    27001 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    27061 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    27121 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    27181 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    27241 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    27301 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    27361 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    27421 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    27481 acttataaat ttattctata ggagacgatt gttttct
//
LOCUS       DAZKAY010000031        27369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000031 DAZKAY010000000
VERSION     DAZKAY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.031"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1889
                     /gene="yfcC"
                     /locus_tag="ACG8WP_001243"
     CDS             150..1889
                     /gene="yfcC"
                     /locus_tag="ACG8WP_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9027558.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2046..3230
                     /gene="iadA"
                     /locus_tag="ACG8WP_001244"
     CDS             2046..3230
                     /gene="iadA"
                     /locus_tag="ACG8WP_001244"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9027559.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3341..3544
                     /locus_tag="ACG8WP_001245"
     CDS             3341..3544
                     /locus_tag="ACG8WP_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9027560.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3645..4307)
                     /locus_tag="ACG8WP_001246"
     CDS             complement(3645..4307)
                     /locus_tag="ACG8WP_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027561.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4320..5822)
                     /locus_tag="ACG8WP_001247"
     CDS             complement(4320..5822)
                     /locus_tag="ACG8WP_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027562.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6370..8049
                     /locus_tag="ACG8WP_001248"
     CDS             6370..8049
                     /locus_tag="ACG8WP_001248"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9027563.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8176..8655
                     /locus_tag="ACG8WP_001249"
     CDS             8176..8655
                     /locus_tag="ACG8WP_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9027564.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8874..9554
                     /locus_tag="ACG8WP_001250"
     CDS             8874..9554
                     /locus_tag="ACG8WP_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9027565.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9562..10893
                     /locus_tag="ACG8WP_001251"
     CDS             9562..10893
                     /locus_tag="ACG8WP_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9027566.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10908..11870
                     /locus_tag="ACG8WP_001252"
     CDS             10908..11870
                     /locus_tag="ACG8WP_001252"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9027567.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11981..12319
                     /locus_tag="ACG8WP_001253"
     CDS             11981..12319
                     /locus_tag="ACG8WP_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9027568.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12398..13105
                     /locus_tag="ACG8WP_001254"
     CDS             12398..13105
                     /locus_tag="ACG8WP_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9027569.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13208..14689)
                     /locus_tag="ACG8WP_001255"
     CDS             complement(13208..14689)
                     /locus_tag="ACG8WP_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027570.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            14998..15534
                     /locus_tag="ACG8WP_001256"
     CDS             14998..15534
                     /locus_tag="ACG8WP_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027571.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15612..16982
                     /locus_tag="ACG8WP_001257"
     CDS             15612..16982
                     /locus_tag="ACG8WP_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9027572.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17049..17384
                     /locus_tag="ACG8WP_001258"
     CDS             17049..17384
                     /locus_tag="ACG8WP_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027573.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17465..18322)
                     /locus_tag="ACG8WP_001259"
     CDS             complement(17465..18322)
                     /locus_tag="ACG8WP_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027574.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18429..18944
                     /gene="tadA"
                     /locus_tag="ACG8WP_001260"
     CDS             18429..18944
                     /gene="tadA"
                     /locus_tag="ACG8WP_001260"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9027575.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18967..19051
                     /gene="ffs"
                     /locus_tag="ACG8WP_001261"
     ncRNA           18967..19051
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8WP_001261"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19261..20646
                     /locus_tag="ACG8WP_001262"
     CDS             19261..20646
                     /locus_tag="ACG8WP_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9027576.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20683..21360
                     /locus_tag="ACG8WP_001263"
     CDS             20683..21360
                     /locus_tag="ACG8WP_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9027577.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21368..22132
                     /locus_tag="ACG8WP_001264"
     CDS             21368..22132
                     /locus_tag="ACG8WP_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9027578.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22134..22793
                     /locus_tag="ACG8WP_001265"
     CDS             22134..22793
                     /locus_tag="ACG8WP_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9027579.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22823..23332)
                     /locus_tag="ACG8WP_001266"
     CDS             complement(22823..23332)
                     /locus_tag="ACG8WP_001266"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9027580.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23444..24442)
                     /locus_tag="ACG8WP_001267"
     CDS             complement(23444..24442)
                     /locus_tag="ACG8WP_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9027581.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24643..25227
                     /locus_tag="ACG8WP_001268"
     CDS             24643..25227
                     /locus_tag="ACG8WP_001268"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9027582.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25304..26479)
                     /locus_tag="ACG8WP_001269"
     CDS             complement(25304..26479)
                     /locus_tag="ACG8WP_001269"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9027583.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26561..26941
                     /locus_tag="ACG8WP_001270"
     CDS             26561..26941
                     /locus_tag="ACG8WP_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9027584.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27015..>27369
                     /locus_tag="ACG8WP_001271"
     CDS             27015..>27369
                     /locus_tag="ACG8WP_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027585.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVP"
BASE COUNT         8868 a         4645 c         5730 g         8126 t
ORIGIN      
        1 aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg attttctaat aaattattca
       61 tgtggaaatg tcatttttac tgtataattc atttgattat ccatttttta ttcatcaaaa
      121 ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac aaattcttca ggaaaaccga
      181 aacgcgactt cactaaatta tcgacacccc atacgtatgt cattattttt ggtgtagtca
      241 tctttgcatg gatactgact tttgtcgttc cagcaggaaa gttcagcacg caagatattg
      301 aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt tttacgtcaa gattccttcc
      361 gttatgctta cgagctggat aagtcgtatg tgtttgatca attagaagaa ctgcaagatc
      421 atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg attggaaaaa gtcattgctg
      481 acggagaaaa aaatcttacg caggaaaaac tagatgaaat ctccttgaca gatgatgtgc
      541 tttacgatca gtacggcgaa aatatctatg atacttctaa aaaattgcat aaaacagcca
      601 aaatatgggg aacagatgat tttggcggct ttggtttctt aaattttgtt tttgaaggac
      661 tagtatctgg agataagtat ggatctgctg tagggatcgc tgcattgatc cttgttgttg
      721 gaggtgcttt tggtattatt atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta
      781 tcagtaaaac taagggactg gagcgtttag ctttaccgct attattcttt gctttttcat
      841 tcggtggcgc tacattcggg atggcagaag aggtaattcc attctcaatg gtgatggttc
      901 catttgtcat tgcgttagga tatgactcga tcgtagcagt aaccgtgact tatgtcgcat
      961 ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag cgtagcggtg gctcaaggga
     1021 ttgctggaat ccccgtgttg tctggtgcga cattccgatt gattatgtgg gtagttgtta
     1081 ctgcattagc cgctggttat atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa
     1141 gttcgttgac ttacaaatca gatgattatt tccgttctca tatcaaaaag acatctgatg
     1201 aaaataaaaa attcatgctt gggcataaat tgatcttatt agagatgtta gttgtccttg
     1261 tctggatcgt ttggggcgtg actcaaaaag gctattacat tccagaaatc gcttctcaat
     1321 tctttgtgat gggattagtt gctggaatca ttgctgttct tttcaaatta gatggaatga
     1381 aagtcaatga catcgcttct tctttccaat ctggtgcagc tgatttagct ggtacagcaa
     1441 tcgtagtagg tatggccaaa gggattcttc ttgtcttggg aggatctgat gcatctgttc
     1501 cttcagcttt gaatacgatt ttgcatggga tcgggacggc gttgaccggt gttccagctg
     1561 tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt caacttggtt gttacatcaa
     1621 attcaggaca agcagctttg actatgccaa tcatggctcc actggcagat ttagtaggtg
     1681 tatccagaca agtagcagtt cttgcttatc agcttggttc aggttttatg gacgctttta
     1741 cacccgtatc agccagcttg attggtgtgc taggtgttgc acggatcgaa tgggcaaaat
     1801 gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt tttaggaact attttcatca
     1861 tgattgccat tgcaataggt ctgcagtagt actggagacc tgtaaataaa aaagtaatcc
     1921 aaacagtaga taaggactcc aagaaccgtc attttacggt aaaatcaaag gagtaaagat
     1981 cctatcgttt ggattatttt ttaagatcct atcatcagtt atacttaaag aaagaggaaa
     2041 aatgtatgaa aatcattcga caaattgaag tgtttgcacc agattactta ggaaaaatgg
     2101 atgtactgat tgccggtggg aagattttgg ctgtagaaga acagctagaa ggcggctacg
     2161 aaggagtgga agtagaagaa ctttcaggtg agggaaaagt actaaccccg ggtttcattg
     2221 actgtcattt ccatatttta ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag
     2281 aagtcacatt gagccaattg actacagctg gtgtaacgac agttgtcggt tgtttgggaa
     2341 cagacggaga aggtagagat atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag
     2401 aaggaatctc cacttatgta tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag
     2461 attctatcat caaagacttt ttgacaattg ataaaattat cggaatcgga gaaatcgcag
     2521 tatccgatca ccgttcttct cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg
     2581 ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat caatgtccac ttaggcggag
     2641 gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac
     2701 aatttttgcc tactcatgca aatcgcacac cagaattatt tgaagcttgc gtggcatttg
     2761 ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga tcctgatttt tgggagaaga
     2821 cagatggtga agttcgcttc agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt
     2881 tagataattt cacgatgacc tcagatggac aaggaagctt gccttatttt gatgaaaaca
     2941 atcatttcct agggttagga gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag
     3001 ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg agctataacc agtaatccag
     3061 cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt
     3121 gtatcttgga taaagaaacg cttgatattg atacagttat cgctaaagga gaaataatgg
     3181 tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa aagtttttaa tcaacagctc
     3241 taactatctt ttttagaaaa cgagcgttct gtttgtctgt atctgttgtt gatgttaccc
     3301 tgaaggtaga tcaataaaca aactaagagg aggaagcaca atggcagatt taaaaggacg
     3361 ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac
     3421 agacgacaag agcaaagaaa cagaagggaa agcacaatca ctaggtgctg acatcaaaga
     3481 aaaagctcga aatttaggcg acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa
     3541 ataaaaacca cttattacta attgaatcaa gcaaataaaa aatccgaacc atatctgcga
     3601 gacagatatg gttcggattt tttatttgat cattatcctg ttttttaaac ttcgattttt
     3661 tgaaattttt tattttttag ttcaaagact tcatcacagc tttttgctac ttcaatctca
     3721 tgagtcacga tgataataca gcgattttgt tcatgggcga tttcttggaa aagtttgacg
     3781 atatcttgtg aagttgtttc atctagattt ccagtaggct catccgctac gattagttga
     3841 tgttcacagc agatagcacg aacgatagcg actcgctgct gctgacctcc cgaaagtttg
     3901 gtcacctgtt tttttgctaa tatttcatca attcctacac gagccagtcg atctaaagca
     3961 acttgtcgat aattctcttg cttggtctta gtgatttcca ttgctgctat gacgttgtct
     4021 agagccgata aataaggaag caagttgtaa gcttggaaaa taatcgagac atcattttta
     4081 cgataacttc ttaacccgat tttgttcagt gaacgatctt gatagtatac ctttccttct
     4141 tttggtgcat ctagtccaga aataagcgaa agaaaagtcg tttttccaga accacttgct
     4201 cctaaaatag cgtacatttt tccagcttcg aaattcagtt ctacatcttt ataaagcgca
     4261 tcttgcggat cttgatacca aaatcccaat tgttctgtcc gtaacatcaa attcctcctt
     4321 tagtttaata ggatttttct tgggtttaat cttaatatcc ctatagaagc taaaaagaca
     4381 gaaaacagac tgattccaaa tgctactccg gctaatagtc ccaattgtgc aggtttgaca
     4441 ttgatttcca attcgctgac ttgatcagaa acagcaaatg gattattcgt ggttccttgt
     4501 tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg gcatttgtcc accgccagga
     4561 gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa gtagctgctg tcccacggca
     4621 ttcgcgacga cgtttccgct aagcgaagca actcctaatg caagaatcat acaaatagcc
     4681 acttcggtaa acaattggag aatgatttta cttctggatt ctcctaaaga tagcaagacg
     4741 cctaactcat gccgacgctc ccgaatggta atcataatga tcagagtaag tatgataata
     4801 ccggctacag ccactagtaa aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt
     4861 tccaacattg actgatacat ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc
     4921 agcttctcag cttctttgag aaactcatcc attttatctg gatcagatag agtgtaaata
     4981 gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa
     5041 gtgtaaagtg tattcgacgg attcatgaag ttgaatttca tacccatact gccgcttgtt
     5101 tcgctggatt cataaattcc tttgatcgtc atttcataag tggtatcttc atcttcagta
     5161 ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg tttctgctag agtagaatca
     5221 atgactacat tgtttgtgcc tttgtcgctg tcatctattc cttcaccatc cgtgattttt
     5281 gctgttccat cagaaaaatt actattttgt gcagttgctg acacaccact gatttgaaag
     5341 tctgcttgag tcatttgcgg attcccgccc attcctccag caggttcacc gcctgcatca
     5401 gtagtttctg aactgtcttc acttgaatca gaactagtga tggcagttat tccatctttt
     5461 gccatagctg aggcggaact ttcaaaggaa taactcttta cattatctaa ttcagctatt
     5521 ttttccgcgt ccgttaagga aactggtgta agagaaaaac tgcctggatc tggacgttct
     5581 tcagtggtag aaccgcttga agaagtttgt tcctgttttt taaatgaagc ttctcgattg
     5641 gcacttaaag tcactgttgc tccaacttct ttttgtgctt gattcgctgc ttcttctgca
     5701 gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa tggctgtgaa cacggcaaac
     5761 aataacaatg aacgtcccca ttttactttt gtacttttcc atgcacgttt cataaaattc
     5821 atattttgca tccctcctag tagatgcatt tgataataga caacctatct taaagctagc
     5881 ttaaatctta tgctgtttgc acaccatcaa catgaggttc tgtatatata aaaagtcact
     5941 atagttattg caaaaaatgt ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga
     6001 aatgaaagag gaatagcaaa agaaatccta gatgctacag agggattttt acgtgcatag
     6061 ttgttctaac aattgctgat actagcgatt tagtttattt gttgtataat aatggggaac
     6121 agctgaatac ttatataagt attctggcta tagcgtttcg caaaagatcc attttgttaa
     6181 tgaagtatat ccaaaataaa atgcccatcc aactgtcaga acgcggatgt gctggtaatt
     6241 ttcctgctga ttctccataa gttgagaaat agtgattttg catatgcttc attggaatgg
     6301 ctcatatatt tagataaaga agaaaatcga catctttaaa cttggatgtc ataagaagag
     6361 gtgaagtttg tgagtaccat taaaattatc ccattaggcg gcgttcgtga aaatggaaaa
     6421 aacatgtatg tcgcagaagt gggcgaggat atttttattt tagattgtgg gctaaaatat
     6481 cctgaaaatg agttgttggg gatcgacgtc gtaatccctg attttacgta tttagaagaa
     6541 aacattgatc gtgtagctgg tgtcttttta acacatggac atgcagatgc gatcggggca
     6601 cttccttact tgttgtcaaa aatttctgta ccagtattcg ggacagagtt gacgatcgag
     6661 ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag gatttaagga tttccatgtg
     6721 gtagatgagc atacagaaat agactttggt gaaacagtag tgagcttttt ccgtaccact
     6781 catacgatac cagattcagt ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat
     6841 acgggagatt ttaaatttga ccaaacggct atcccaatgt atcaaacaga ttatgcacga
     6901 ttggcggaaa tcggcaaaga aggagtctta gccttactaa gtgaatcggc aaatgcagaa
     6961 aatcctcagc ctgtagcttc tgaagcccaa atagcagatg aagtatttga tacgatcaaa
     7021 tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa
     7081 gtattggatg cagcagaaaa agcacaacga aaagttgtct tgactggtca agatttcgaa
     7141 cgaattattc gcacagcgat gcgtttagaa aaacttcaat taccaagtga agatctgctt
     7201 gttacgttga aagacatgaa aaactatgat ccagaagaac tgatcatttt agaaactggc
     7261 cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc
     7321 aagattgaag aaggagattt ggtttatatc actactacgc caagcatcgc gatggaaacc
     7381 atcgtggcga aaacggaaga tatcatttat cgagctggtg gaacagtgaa gttgatctct
     7441 gagaatatgc gcgtttctgg ccatgccaat cctaatgact tgcaattaat gatcaatttg
     7501 atgaaaccaa catattttgt gccagttcaa ggagagtatc gtgaattagc tgctcatgca
     7561 gatttggctc atgcagtagg gatgccatac aaaaatatct atattactgg acgcggagac
     7621 gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta
     7681 atgattgatg gattaggcat aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc
     7741 ttgtcagaag acgggatttt cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc
     7801 atttcaaaac ctcaaattac ttcacgggga ttcgtttacg taaaagctag ccgtgatctg
     7861 atgaaagaaa gcagcaatat gattgaaaca atcgttgaga aacatttaga aagcaatgat
     7921 tttgagtgga gcaaattaaa gcaagacatc cgagatcaat taggccgtta cttatttgaa
     7981 caaacaaaac gccgcccagt gatcttgcca gtcattatgg aagctactca acgtcgaaat
     8041 aaaaaataaa caaaaagagt aagatgaacc tctctataga gtgctcgtct tgctcttttt
     8101 ttgcgaaaaa caaataattt ttgaatacag catccctcgc tttataataa acgatagaat
     8161 aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac gttatttaga agaacaaaag
     8221 atcagctatc aaccacttga ttttagtcat gtggtagaag aatctttcac agaagaacta
     8281 caaaaaagag ggattgatcc agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa
     8341 actggggtga tcatcgcatt gattcctttg aacgatcact tagattacaa aaaaacgaga
     8401 caagtgacga aagatcgtaa agttggtttt cctggaatgg attttgtatt aatgcataca
     8461 ggatatcccc acggagcaaa cacccctatt ggaataaaac tcgcacatcc tgattattgt
     8521 tttttagcag acagttcttt aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc
     8581 gggagaagcc ttcggataaa agtagccgat ttagaaaaga tcgtccatcc tatttacgct
     8641 gatttaatta gttaagggac gagaagagaa gctaagcaga gcagagacct caaacttgag
     8701 attatatctg cttggtactt ggtgcatcct gtattatctg ttacaattgc tttgtacata
     8761 ctgtactatc aatccaatga aaatatattt agcagctata ttttttcatt gatgtgcaag
     8821 tgatgaaatc gtttttaata aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa
     8881 ttacgtatca tggacattcc tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga
     8941 ttgacccgtt tattacagga aatccattga cagacttaac tgcagaagaa gtaaagacag
     9001 attggttgct gattactcat ggtcatagtg accacatagg agatatgatt cctattgcaa
     9061 aagaaaatca agccacaatt atcagtattg ttgagattgc tgattatgcg aacagcagag
     9121 gagtagattc attcggtatg aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa
     9181 aattcgtaca tgcacagcat agttcaagct atgaagtaga cggtatcgtt cagtatatgg
     9241 gcgagcctag cggaattatt attcaagcag aaggaaaaac gatttatcat gcaggggata
     9301 ctgcttattt cagtgatcta ggattgcttg cagaagaatt cgatattgat gtcgctttct
     9361 tgccgattgg cgataactat acgatggggc cagaagatgc agcaaaggct gcgcaaacaa
     9421 ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt ccctgtcatt gaacaagatc
     9481 ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa aataatgagc gtctcggaaa
     9541 cgctggaagt gtaggcagaa aatggcgaca aaacatgatt tgattttaga atatattgaa
     9601 agtttacctg ttggcaatcg aatttctgta agaagcatcg caaaagaatt gaatgtcagt
     9661 gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg tcggactagt ttcaacaatt
     9721 caacgagtag ggaccatccg tatcgaacgc aaattaaaaa aacatattga acgtttgaca
     9781 tttggcgagg tcgtcaggat cattgaaggg gacgttttag gcggggcggc aggattagac
     9841 aaggtcttga ataaattcgt catcggcgcg atgactaaac atgccatgga acgctatatt
     9901 acgccaggat cactaatgat cgtcggcaac cgggcggaag tccaaaaatt agcattggaa
     9961 gatggcgccg cagtattgat cacaggagga ttcaatacga ctgctgagat tgcaaagcta
    10021 gctgatgatc ttgaaatgcc gatcttacgc acctcttatg atacatttac agtcgcaacc
    10081 atgatcaacc gagcgttaag cgaccaattg atcaaaaaag atatcatgct tgtcagtgat
    10141 atctatatgc cagctgaaaa gactcattat ttgcagcaga cagatacgat ttctgattat
    10201 cagcgtttgt cagaagaaac gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt
    10261 ttgatgggga ttgttacggc aaaagatgtt ttagggaaat cgccaaacca gatcatcgat
    10321 cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa tgagtattgc ctctgttagc
    10381 catcaaatga tctgggatgg gctagaagtc atgccggtcg tttccgatga cttgacattg
    10441 gaaggcttga ttacccggca agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg
    10501 cagattgcag atacgatttc ggatcagata tctggaaata tccatacgat tgatactgac
    10561 cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg tagtagcacc acaaatggtc
    10621 aatggcgttg gaaccatttc ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa
    10681 aaaacaatgg tgatgaacca aaaacgaaat atcttgattg aacaagtgaa cctacattat
    10741 ttgcggctga tccaattgga aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc
    10801 agacgttccg ctaaactgga tatcgaagtg tttattgaaa acaccatcgt ttccaaagca
    10861 atcgttgttt gtcaagtaat ggaacgttct tagaaagcag gagtgaaatg acagtacagc
    10921 aagaaatagt agatacaata aaaaaatacg ataaaatcat tatccacagg catttgcgcc
    10981 ctgatcctga tgcattagga tcacaagtag gattagccga actattacgg gaaagtttcc
    11041 cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa
    11101 tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt catcgtgact gatacagcta
    11161 attcacccag aatcagtgat caaagatatc agttggcaga aaaactgatc aaaatcgacc
    11221 atcatcccaa tgatgaccct tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct
    11281 gtagtgagat gattgcagaa tttgctttga tgtttcctga atctttaaag atgaacgtta
    11341 aggctgcacg gttactttat gcaggaatcg tcggagatac agggcgcttt ttgtatccct
    11401 ccacgacgtc ccgtacatta gagattgctt cgatattgag aaattatcca tttgatgcaa
    11461 gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt cgctaaactt tccggttatc
    11521 tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa
    11581 ttcttgataa atatggaatc gatgattcag agacagcttc gatcgtttca cttcctggag
    11641 tgattgacga agtgctggct tgggggattt ttgttcagca gccttcaggg tattaccgcg
    11701 tacgtttacg ttctaagggg ccgatcatca atgaattggc taaacgccat catggaggag
    11761 gccatccatt agcaagtggt gcgaatgcaa aagatttagc agaagtagag gaaatctata
    11821 aagagatcca agatatatgc caatcgtata agagcagaaa aacagaatga tacttcgttc
    11881 gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt ttcaagatct tctttttccg
    11941 ttacaatagt gctcactaaa gattttaaag gagaaagaag atgacattac caaattgccc
    12001 agaatgtggc tcagagtaca catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg
    12061 gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa gacggattag tggtattaga
    12121 tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca gtgattaaag acttgaaagt
    12181 aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga
    12241 aggcgatcat aatatcgatt gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga
    12301 atttgtgaaa aagaattaaa gaatattcaa ggtcttttca cattcttttc ctactactaa
    12361 tgataaaata aagtaaacga aaaggagtga aacgaaaatg tatggttatg gaatgtatgg
    12421 ttttttagat ccgacatttc ttttggtcat tcttggatta gttatttccg gaattgcgtc
    12481 agcgtacgtc aatagtactt tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac
    12541 tggtacacaa gcagcacaat atatattaca aagtcaacag ataaataatg tcggtgttca
    12601 acaaattgcc ggagatctca cagataacta caattcaggt aataagatgt tgagtttgtc
    12661 tcaagcaact gcacagtcta cttcagttgc tgcgataggt gtagcagctc atgaatgcgg
    12721 acatgcggta caagatgctt cgggttatgt cccattgaag attcgtgcag cacttgttcc
    12781 agcagctaac tttggttcga tgatttcgtt ccccttgatt atggtaggtg tattgcttag
    12841 ctggaactct acgttaatca atatcgggat tttcgctttt tctttagcat tacttttcca
    12901 attggtgaca ttaccagtcg aatttaacgc gtctcggcga gcaatccaaa ttttgagtga
    12961 aggcggacta ctgacggaag aagaagtacc gatggcaaga cacgtattat ttgcagcagc
    13021 actgacttat gttgcagcag cgctttcgac attcctgcag ctgttgcgct tgatcttatt
    13081 gttcggcggc aaccgaagag attaaaataa agaagttgtg aaatcagcgc tttgatctga
    13141 gctaataccg agagttagct gattgaatac catttatttg gaataagagg atgtgacaag
    13201 aattttgtca catcctcttt gttttgttct cataaccttt tttcatatac agttcatgaa
    13261 ccgcttttat taacttgcca aataacactg tattgtgcgt tgtatccaga tagaaagtct
    13321 ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat caaatcagca tgttctgaat
    13381 atgcggtaat tttaacagca tctttgcgaa ataactgctg caaatggctt tgaagatgat
    13441 aatttcctgt aaactgtttg gttatctgca aatggatgag taactgctct ttcgtttctg
    13501 tttgtagtaa gtgttggatc aattgactaa ggtattgact gatttggttt ctttccccat
    13561 tttttgaaaa aattgtatcg attttttctc ttaatttctt ttcctgtata aaagaactag
    13621 aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa actactgctg gattcaccca
    13681 acagttcgaa aaaaacaact gccaacacaa gaaaataaaa ataaattggt attttatttt
    13741 ctaaatggtt gagaggaaac tgttgagtta agtaagtcat caattgaccg acttgtcgac
    13801 aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga
    13861 tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta ggctctctct gtttctgtct
    13921 cttcacaagg gcgactcact gaaaaagaag taaagtattc tgaataatca tgtgtatgct
    13981 gcaataagtc catcaattca gtgatttcag aagaagtttt tggaaaatat gcctgttgtt
    14041 tgtttcgtaa tatgaataaa gttaacagca agtagatatt tttttcttgt tttgaaattt
    14101 tttctatttg ctcatctgta ctgttcattt ttagtgcatt taattcaagt gaggaaaatg
    14161 gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta aagatagaga cggatagata
    14221 actcatttcc ttcaagagta cagcaatttt ttacagatag gatccgtaaa tggaatttta
    14281 ttaaggaatg attgagctgt tttgtcagtt tttcgagata ggagggcgat atataaagtt
    14341 gttcaagtaa atattttttt ggtacagcag tttgtggata attgattagt aacatcaaat
    14401 agttaaaacg attggattct tttaggtagc ataattttaa cttagaaaaa acagctaact
    14461 ttttagtttg atcggtttct ttggtttgga tctctccttt atgattagaa taaataaatt
    14521 catcaatcaa taaaaattgc ttgagtgata catttatttt tcggatcagt tctttgattg
    14581 tccgttttgg aatatgtaag tcattggata attcttgtaa agaacaatta tttttttgaa
    14641 tgaccatttg tagaacaagt aactcttttt ttcttgcttc aattagcata aatacctcct
    14701 ttttattctg tttttgtata tagttagaca aaaactgtga gtggaaagtc atttcagtct
    14761 gtgttattct cacagagtgt cgccttcttt tttatataac gttatttaat ttttttgtta
    14821 ccttgtttat tctattgttt atttcctagg gtattttgtc aagatattct tgtttttaag
    14881 gcggaaaaat tgatttttaa tggatataac taaatatgtt ttgataatta tcaataatat
    14941 ttttagaatt atttatgtta tttatttatg ggtagatgaa aagagacgga ggtaagaatg
    15001 atggggagag taacaaatta tagtaaagaa tatttgctgt tcaaaagtat ggtatacgtg
    15061 gaagaatatg gaatgaagtc aattactgca agagagttag cagagttctg cggttgttct
    15121 acatacccga tttatacaca tttcaaatca atcagcgggt taaaagagaa gatattagaa
    15181 gaaatcactg tttattttga aaactactta gctgaatgca gttccgatga tgttttaagc
    15241 attgtctact tattgaaaga ttttttttct atccatgaaa gtattcgtga aatcattgca
    15301 aaatctgatt tagacatgga ttcgctattt aagcggccat ttttctcata tgtcaaaggt
    15361 cacacgaaga tcaaagaaga aaagtcgctt gaattggtat ggcttaatgc attgggaagt
    15421 ttgcgctcag atgattcttc tcaagatttt cttgaaatga tgaacgctgt ttgggataaa
    15481 aacgaggtga cggatgaata cttggaaaat atcttaaagg aacagtcacc ttaggcagca
    15541 acatgaaaaa gtagatcagc cacctttatt tttcgtaaaa aagattgtaa aatgtctaac
    15601 gaaacattgg aatgaaggag gaagcgacaa cgttaccaca gcaatttatc aaaaaatatc
    15661 gtcttctttt aggcgaagaa gcatccgatt ttttttctgc gcttgaacaa ggaagcgtga
    15721 aaaaaggatt tcggtggaat ccactaaaac cggcaggatt ggacatggtc caaacttacc
    15781 atagcgaaga attacaaccc gctccctaca gcaatgaggg atttcttggc actgtcaatg
    15841 gaaagtcttt cctccatcaa gctgggtatg aatatagcca agaaccaagt gcaatgatcg
    15901 ttggaacagc tgctgcagca aaacccggtg aaaaagtttt ggatttgtgt gcggctcccg
    15961 gtggtaaatc aacccaatta gctgctcaaa tgaaggggaa agggttatta gtgacgaatg
    16021 agatctttcc gaaacgtgcg aagattttat cagaaaatat agaacgatgg ggagtttcga
    16081 acgcaatagt gacaaatcat gcacccgcag aacttgtccc tcatttttcg ggcttttttg
    16141 atcggatcgt ggttgatgct ccttgttcag gagaaggaat gttccgaaaa gatccaaatg
    16201 caataaaaga gtggacagaa gaatctccgc tatactgtca aaaacgtcag caagagattt
    16261 tgtcttcagc aataaaaatg ctaaaaaata aaggacaact gatttattcg acttgtacgt
    16321 ttgcaccaga agaaaatgaa gagatcatta gctggcttgt agaaaattat ccagtaacga
    16381 ttgaggagat tcctttgact caatccgtta gttcaggacg ttctgaatgg ggaagcgtag
    16441 ctggtttgga aaaaacaata cgtatatggc ctcataaaga tcagggtgaa ggacattttg
    16501 ttgcaaagct gacttttcat ggacaaaatc agatgcataa agaaaagaag acacgaaaaa
    16561 aaagtaaagt gcaaatgaca aaagagcagg aaaaactctg gacggagttt tcaaatgatt
    16621 ttcattatga agctactgga agacttttag tgttcaacga ccatctttgg gaagttcctg
    16681 agttagcccc ttctctcgat ggtttaaaag tcgtacgtac aggtttgcat ttaggagatt
    16741 tcaaaaagaa tcgatttgaa cctagctatg cattagcgct tgctacaaaa aagatagaga
    16801 atataccttg cttgccgatc actcaaaagg aatggcaatc ttacacagca ggagaaacat
    16861 ttcaacgtga tgggaatcaa ggatgggttt tgctagtatt agataagata cctgtcggat
    16921 ttgggaaaca agtaaaagga acagtaaaga actttttccc taaaggattg agatttcact
    16981 gagtggatta gttcgtttat ttttttgtct atgctatgat ggagatagat aaaaaggagc
    17041 tgatacttat gaaaacatca acaaaagtaa caatcggatt aagcgtagca gccgctgcca
    17101 gtgtagcgac agcagtcgtt gtatcgggaa aagtaatgga aaagatccat catatgacta
    17161 ctcggacgaa agtaaaaaaa ttcgtctatg acaaatttga tggaaatgaa aaattactag
    17221 aaatcgtaga tggtctttca gatagtgact tggattcttt gatgggaatg ttagcaaaaa
    17281 tcaaatcagg aaaaaagaaa atctctgttt atggtgattc aatcaaagat tcaaccgaag
    17341 atgtaaaaga ccggctaatg cattttgttg aaaaaatgat gtaagagaaa ctagcttttg
    17401 aacactattc attcgaaata aggggttgtg acaaaattat tgtcacaacc cctttaatct
    17461 atctttaatt atgttcatga agcaataaaa gatagcaaaa cagtaaatcg ttggcttttt
    17521 ttagattgaa accagactgt tcttgaaatt tttcgattcg atattttaga gtattacgat
    17581 gaagaaacag tgtttttgca gcagagctga cattgcctaa ttcattccat aattcttgaa
    17641 tgatcacttc catttcattt tgtttgatca gttctatgta acttttgatc aacttactat
    17701 cttttacagt atcttttgta tagtaatgta aagccacatc ttgcatagaa aaagtacggc
    17761 tgtgagtata aagattgttt tgctctgtta aaaaaatttt tctttcttca gcaaacaaag
    17821 ggacgaggtc ggtgccaggt gaatggaaag aaccaacgaa tacagacgtt gtcgtatcaa
    17881 aatcagcatc taaagataga aaaataccgt tgatttcagt agcatcaaga taatcacggt
    17941 ttatttgttc gactaagagc gcatctgtgt ctgtataaaa aaagaaatca gcagaatagc
    18001 gaaataattc caaaatagta tcttgccatt ctttttttaa gaattctcct tttgattcga
    18061 cgtgaagctg gatcaaacga aagctttttt cttgaacaca agaagcatct ttaaaaagat
    18121 agtgatacca tgggtgtttt ttttgatcgt tgattacagg gaataacgct ttcaaaaggc
    18181 tggtttcttg ttgagataat gaatcttgtt tgatccagat aaaagcatca tcaattgcaa
    18241 aagaaaggat atccgcttct gatgctggag agtttttttt ctgtgcttgt gggtaaagag
    18301 caagtaattt atccattttc atcttgttca cctcttgtat ttattttacc atagaaaaaa
    18361 aagttagtta cagccttttt atcatatgct aaagtaaaac aagaaaaaac aaaggcaggg
    18421 aagggtaatt ggtaaataca aatcttgatg cagtagaaaa agaaaaatgg atgcgccttg
    18481 cattggcaga agcaaagaaa gcagaaatgc ttcatgaagt tccgattgga gcagtggttg
    18541 tattggatgg aaaagtgatt ggaagaggat ataaccttag agaaaccacg caagatgcta
    18601 ctacacatgc agaaatgctt gcaatcaagg aagcctgtga aaaagtggga agctggcgct
    18661 tggaagatgc tgcacttttt gttaccttag agccttgtcc tatgtgcagc ggcgcgatga
    18721 ttttgtctcg tgtagcagaa gtttatttcg gcgcgtatga tccaaaaggc ggaacagccg
    18781 gtaccttgat gaatcttttg gaggacgagc ggtttaacca ccaagcatat gtagaaggtg
    18841 gcattttaga agacgaatgt gggatgattt tgactgactt tttccgaaag ctccgccagc
    18901 gaaaaaagaa attaaaaaaa gactggtcaa cgaatgaaaa ataaggtata ctattctttg
    18961 ccgaaaggct aggacaatgg tgggcttgtg aagcgtgtca ggtctggaag gaagcagcac
    19021 taagcaggtt ccgccatgtg tctgattata atgaaacgac aacagacttt cgggtctgtt
    19081 ttttttattt ttatagtact gtaaaaatga atgttcctga ttttttagcc agactgattt
    19141 atcgaatatt gtacttatac aatttgcttc aaggcaggaa aaactctgaa atagagcttg
    19201 tttcaatgga agaaaaagct gcctatggta tgataaagat aaagagaggc gagagggtac
    19261 atggaaaaaa tagtgatttt acatacgaat gatctgcatt ctcatttaga aaattggcca
    19321 aggatcagaa ggtttttgga tcaacgtaaa cgagaaaacg aaaagaaaga aaaaacgaca
    19381 acgattgcag tagatctagg tgattttgtg gatcgttggc atccattgtc agaagcaacg
    19441 aatggacagg aaaatatctt gctgatgaat gaagttggtt atgatgcagc gacgatagga
    19501 aacaatgaag gcgtaggaaa ttccaaagaa gagttgaatc atttatacga tcatgctgag
    19561 tttgatatca tcctggataa cttattcgat aaaaatacgc tccagccacc ggcatggacg
    19621 aaaccatata aaatcattac aacgaaacac cagacgaaaa tcggactaat tgcatttact
    19681 gctccatttc cgcttactta taatccgaat ggctgggaca tacggaatcc ttatgatatt
    19741 ttgccagaat tagtcgaaga tttacgttca caagtggatg ttttagtttt gatgagtcat
    19801 ctcgggattc aggatgattt ccaaattgca aaagaaatac cagcaattga tgttatttta
    19861 ggatcgcaca cccatcattt attcagagaa ggtcacgtaa tcaatgatgt ccaaatagca
    19921 gccgctggaa agtacgggca gtatgtcggt gaagtccatt taaccatcga tgaagagaaa
    19981 aaaatcctta agcattcagc caaagccatc cctaccgaaa caatgacagc ttttacagaa
    20041 gatgaacaag aaataaatag ttatctagaa cgtgggcatg aattattaaa agaaaaagaa
    20101 gtagcagatt tgccattttc tctgagtcta gatatcttca atgagcattc gtttatccaa
    20161 gtagcctttg aggcggtgaa agaacgagga aagacggaag cagcaatatt aaacagtggt
    20221 ctgtttttaa ctgaaattcc aaaagggcgg gtcaatcagg atcagcttca cactgcgttg
    20281 ccgcatccga tgcacttgtt aaatgtcaca ttgggtggaa atgatctgat ccgcttagtt
    20341 ttagaaatag aaaaaaatcg taacttttta cgtaactatc ctatgaaggg aatgggattt
    20401 cgcggtaaga tttttgggca gatggtctat aatggaatca catatgattc tattaaccat
    20461 caagtctttt ggaagaacaa gccaatcgat ctgaataaaa attatacttt tactaccgta
    20521 gatcatttga tgtttgttcc attttttcca acgattgaga ttgctggaaa aaatgaattt
    20581 ttgttccctg aatttatccg aagtgtggta ggagattact tgaaagctca ctacccaatc
    20641 aaataaaaac aaggtataat aaaaaagata ggtggtgaac gcatgtctgc aaaacaaaat
    20701 gaacagcaga acgttgagca taaaacagaa attgctaaga aaaaatcatc agctgaacag
    20761 gaaacaacag tcaccgaaga actgacagct gctttggaag aaatcgttga ggagcctaaa
    20821 gcagaaaaag tcaaaggcga gctagtgaca ctgttagatg acagtaattt tttgataggt
    20881 gaaagacatt atcggcttgt gtccgattat cgtgaaggat ttaatgcaga aaagcttgga
    20941 gaacgttaca gtgatgtact agcccgatat gattatatcg taggagattg gggttatgaa
    21001 caactacgcc taaaaggatt tttccaagcg gataatcgac gggcgcatcc agatcagcga
    21061 atcgattcct tggaagacta tctttacgaa tattgcaatt ttggttgtgc gtattttgtc
    21121 atcgagagaa tcggcggtaa aaaagaaaag acaactcagc gccgaaaaaa gaaaaaaaac
    21181 cataatcgtg cgtatatcga tgagaaaaaa ggaccagtcg catctaatcg caaaaaaccg
    21241 gttattaaaa atcgaaaaac agagtcagca aaaacctctg aaaaagtaaa aaaacaagaa
    21301 caacctaaac aagtagaaaa aacaaaaaca gcatttacga tcagaaaaag ggaagagtag
    21361 tggatgaatg gactataaag gatatttaat tgatttagac ggaacgattt atcgtgggac
    21421 acaacccata ccggccggta aacgatttgt ggaagaattg caaaaaagaa aattaccttt
    21481 cttgtttgtt accaataaca cgactaagtc accagaaaca gtagctaatc gtctagcaga
    21541 tgagtttgac attcatgtag ctccagagac agtctacaca gctactttgg caacaatcga
    21601 ttttatgaaa gcagatggaa aagggaaaaa agtctatgtt atcggagaag caggattgat
    21661 tgacttgatt ttagctgccg gatttacatg ggaagaagag gctcctgact atgtagtggt
    21721 aggcttagat aattatctga cctatgaaaa agtagttaaa gcaacacttg ctattcaaaa
    21781 aggtgctact ttcattggaa cgaacccaga taagaatata cctactgaac gcggccttct
    21841 tcctggagca gggtcagtca tctcttttgt agagacagca acccaaacgc cgccgatcta
    21901 catcggaaaa ccagaagcca tcattatgga taaagccgtt gaagtattag ggctgcaaaa
    21961 agaagaagta atcatggttg gagataacta tgaaacagat atccaagctg ggatacgcaa
    22021 taatatagat actttgctcg ttttatcagg ttttacaaaa gaagaagatg tacctgggtt
    22081 gcctatccca gctacctatg tgaaacaatc actggatgaa tggagcttct aatatgaaaa
    22141 attcgagatg gcagtggatg gaatatgctg gattgttcag cctatttctg acactgatca
    22201 gtctggcagt tggtgtgacg atcaactttc gaccactata cgtatttgat atcggacatt
    22261 tgcaaatttt ggattatact tctttagatc aagagacgtt actgaaaaat tttgatcacc
    22321 taatgaatta tctcaataat cccttcaaaa ccatcctcag cttgccagat ttcccagttt
    22381 ctgctagcgg agcccatcac ttttatgaag tgaaaatttt gtttttagtg gattatgctg
    22441 tgtttttcat cactttgatc ccgagtattt tgtttatcaa atatttgcaa aagaatgatc
    22501 ggctttggcg gttgattcgt ccgtttcaaa tcggcatgtt actgccagtt gttttcggtt
    22561 ttttcatgat gataggattt gatcgctttt ttatcctttt tcatgaaacg tttttcaaca
    22621 acgatgactg gttattcgat cccgttactg atccaatcat caatgtactt ccagaagaat
    22681 ttttcatgca tagctttatc ttattttttg tattattaga attatttttt gctgtttttc
    22741 ttttcttagg aaaaaatagc ttgaaacaaa caaaaaagaa agaactcgtc taaatagacg
    22801 ttttctttct ttttttgttg aattattgag taaggcgact tcttttttct ggttctttgt
    22861 gtgctaatcg actggcagca gcagcggcga tcgctccgac gatatcatct aagaaggtat
    22921 ggacatgaac gccatcgtgg ctgtttagtt cttttaaaat accaggtttc accttgtcga
    22981 tataaccata attcgtaaag ccgatcgtac cgtaaacatt cacgattgac aaggctagga
    23041 tctcatcgat accgtaaagg ccttcgtcat cttcgataat atgttgcaac ggactcatca
    23101 attttttctt ttctgctaat atatctaatt ggattccagt gataatggcg ttatgtactt
    23161 cacgctttgt tagcacgctg ttgacacttt cagtacaatc cgccaaagtc aaatcagaaa
    23221 tatagtcttt ttgtaaaaac atgacaagtt gtgcgatatc ttctacagtt acgccacgtt
    23281 ccttcaatag ttctcttgcc ttttcttcta aagtctcagt tttgacgacc atattattgc
    23341 tcccccttta ttttttcttt ctctatttca tgttaacgga acatgaaaaa aatgcaagaa
    23401 actgctttcg ggtttaactt gaaatgcaaa gagatataaa aaatcaacta ggacgaagat
    23461 ttttatcgta taagcttgtg ctgtgaccag gctgagttcg ttcctttgga ttaatatagt
    23521 gtaaagcgtt gttcactgca gtaggtgctt caccgagccc agtagcaatc agtttcactt
    23581 ttccttcata ggtacagatg tcaccagctg cataaacgcc tggaatagaa gtagacatat
    23641 ctgattttac agtaatggca tttctggtac tatcaagtcc ccaagaagaa agatgttcta
    23701 aattagaaga gaatccataa ttgacgatca gagaatcaat catcaaatca atcgtctcgt
    23761 cacttttcac tttttttaac cggatatctg tcagttcccc attattgcct gataatccat
    23821 cgatgagata gggagtaagc aagttgacag atgaagattt caagcgtgat acactatgtt
    23881 cgtgcgcacg aaattccggg cgacgatgaa tcaagtaaac ttcgcttgca ataggctcta
    23941 acatcaacgt ccaatcgata gcagaatcac cacctccagc aatagcgact ttttttccag
    24001 catacttcat caaatcattc acgaaataat ccaatccgtg gttttcaaat gattcagcat
    24061 tatctaaatt caattttctt ggctggaatg aaccatttcc taaagctaaa atgactgctt
    24121 tggaataatg gatgccttta tttgtagtaa tctcgataac ttcgtcttca cgagtaagcg
    24181 agagaacttc ttcttttaaa tggaatgtat ggttaaaagt agttaattgt ttttctaatt
    24241 gatcgattaa ttcacttgct tttattgctg gatacccagg aatatcgtaa atatattttt
    24301 cgggatacaa agttgctaat tgaccaccaa gttgaggtag actgtcaatg atttttgttt
    24361 tagcttggcg cattcctgcg taaaaagcgg caaacatccc tacaggacct gcaccgatga
    24421 ttgtaatgtc atagatttcc ataaaaattc ctcccttttc acactatatc agaatttaat
    24481 tgagaatgct tctcaaaaat gtttgaattc gaaatcgaaa cggtaagtta tagaatgtat
    24541 ctagtgatga aaaataaaag aaaacaaatc tagtattttt acgagaggtt aggtattatg
    24601 ttattattat tatgaagtat gcaaatatta ggaggaaaca atatggcatt cccacaacta
    24661 gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa caaaccgtgg tgacattact
    24721 gttcaattat tccctgaact agcaccgaag acggttcaaa atttcattga actttccaaa
    24781 aaaggttatt atgatggcgt gatttttcac cgcgttattc ctgatttcat gatccaaggc
    24841 ggcgatccaa ctggaacagg aatgggtggc gaaagtattt atggcgaaag ttttgaagat
    24901 gaattcagtc gtgaactgtt caaccttcgc ggagctttat ctatggcaaa ttcaggtcca
    24961 aacacaaacg gaagccaatt ctttattgta aataacacaa atgtgccagc taatatgctt
    25021 ggacaactag aaggtgctgg attcccatca gaaatcattg aagcgtataa aggtggcgga
    25081 actccttggc tagatttccg acatacagtc tttggacatg tactagaagg gatggacaca
    25141 gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc gacctgtaca tgatgtagtg
    25201 attgaaacaa ttgaaatttc agaatagttt ggtatggtac ggagtaatta tgaaaaagga
    25261 cgaaacctag taaggtctcg tccttttatt ttgaataaat gatttaatcg attgtctcta
    25321 aagcatactt gattcgatta agtccttcca gtaaagtttc ttctggacaa gcaatattca
    25381 agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc aggatttaat acgacttttc
    25441 ctttccgaac aagagtgttc tccaattctt ggtcgtctga aatataggcg gtaaaatcta
    25501 accacattaa gtaagttcct tcagggttca ttattctcac tttcggaaga tactgttcaa
    25561 aaaactgatt aactgtttga acattttttt ctagataagg aatcagttgg cttaaccatt
    25621 ctccacccgt ctcataagcc gcttgggttc caatcagtcc aaaagtgttg atttcgtgct
    25681 gttggttcat cactaaatgt ttttcaaaat cagctttcat ctctggattt ttgataaata
    25741 ccattgaatt tttgattgca gctagattga aagtcttggt tgcggaagta aaagcaacta
    25801 gtagatcatc taaattagga tcaatcgtta gcatggaagt aaatgtatga tcgaataaag
    25861 ttaaatcttg atggatctcg tcactaaaca gaatcacttg atgttttaaa cacaattcgc
    25921 ttaactgttt caattcttct ttgctccaaa cacgccctcc aggattgtga ggattgcata
    25981 aaatcattgc ttttacattg ttttcttcga tttttttctc catgtcagac aagtccatga
    26041 tgaaatgatt atttttttct agcaaagagc ttcgaacgat gtgacgttga tttgtttcta
    26101 taatagagga aaatggagga taaactgggt catgaatcaa aatagaatcg cctggttttg
    26161 taaaagtttg tactgctgca gctaaactgg ttaacacacc acttgtaaaa acgatatttt
    26221 ctttcgtcaa ctccacagca tggtgttggc gttcccaatg aatgatagcc tgatatagtt
    26281 tatcagacag agtggcataa ccaaagattc catgtttgat gtattcacta aacgctttag
    26341 ctaccccacc gggagacaag aagtccatat ctgctaccca caaaggaagt aagtcatcca
    26401 tttgatatgt ctcagcaatt gcgtcccatt ttacactttc tgtatctttt cttgaaatct
    26461 gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt cgtcgcttct ttttttgagc
    26521 tatttacagt ataatgataa atgcgaagag agggaagtag atgacttata aaattggaca
    26581 aataattgaa ggaaaagtga cagggataca gccttatggc gcattcgttt ctcttgataa
    26641 cgagacgcaa ggacttattc atgtttcaga agtacaatcc ggttatacga aaaatatcca
    26701 ttcattgtta aaagtcggac agccagtaac agttcagatc atcgacattg atgaatattc
    26761 caaaaaaatc agtttatctt taagaacgtt agaaaaaaca aatccagctg ttccatatcg
    26821 cagaaaaagg tatttcacga ataaaaatcg taagatcgga tttaccacta ttgcggagca
    26881 gttacctatt tggatagaag aagcattatc tgaactagaa cggaaagaaa ataagaaata
    26941 aaaagtgtat tttttctgaa aatgtgctag aatttttatg taagccatag aacaagttga
    27001 tcgaggtgga aattttggca agcaaaagag ttgctggtac aattatgttg catttggagg
    27061 atggtactaa aaaatttttg gtccattcaa ttgatgacaa gttggaattt gcgttagcgg
    27121 aactttctga aggaaagact ggtttagcga atatacttaa tttcttaaaa gaaatagttc
    27181 atatcgatgt tagcaagatt agtttgatgg aattgacaaa tacacatatt aaagaggaaa
    27241 atgttccgtt gtttgtcttt gaaactgaac aaaagaatca aagagaagaa ttaggcgaag
    27301 gatatatttg ggaagaacca ggtcaaatgc gctctgttct tggaacttac gaagtagagg
    27361 gagtgcctt
//
LOCUS       DAZKAY010000032        26817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000032 DAZKAY010000000
VERSION     DAZKAY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.032"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1119
                     /locus_tag="ACG8WP_001272"
     CDS             115..1119
                     /locus_tag="ACG8WP_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027586.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1120..2064
                     /locus_tag="ACG8WP_001273"
     CDS             1120..2064
                     /locus_tag="ACG8WP_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027587.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2064..3026
                     /gene="opp4B"
                     /locus_tag="ACG8WP_001274"
     CDS             2064..3026
                     /gene="opp4B"
                     /locus_tag="ACG8WP_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9027588.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3053..3967
                     /locus_tag="ACG8WP_001275"
     CDS             3053..3967
                     /locus_tag="ACG8WP_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027589.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3993..5774
                     /locus_tag="ACG8WP_001276"
     CDS             3993..5774
                     /locus_tag="ACG8WP_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9027590.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6122..6808
                     /gene="rnc"
                     /locus_tag="ACG8WP_001277"
     CDS             6122..6808
                     /gene="rnc"
                     /locus_tag="ACG8WP_001277"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9027591.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6828..10409
                     /gene="smc"
                     /locus_tag="ACG8WP_001278"
     CDS             6828..10409
                     /gene="smc"
                     /locus_tag="ACG8WP_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9027592.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10418..11227
                     /locus_tag="ACG8WP_001279"
     CDS             10418..11227
                     /locus_tag="ACG8WP_001279"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9027593.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYGQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11238..12236
                     /gene="ftsY"
                     /locus_tag="ACG8WP_001280"
     CDS             11238..12236
                     /gene="ftsY"
                     /locus_tag="ACG8WP_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9027594.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12423..14690
                     /gene="gshAB"
                     /locus_tag="ACG8WP_001281"
     CDS             12423..14690
                     /gene="gshAB"
                     /locus_tag="ACG8WP_001281"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9027595.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14779..15231)
                     /locus_tag="ACG8WP_001282"
     CDS             complement(14779..15231)
                     /locus_tag="ACG8WP_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9027596.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15381..16331
                     /locus_tag="ACG8WP_001283"
     CDS             15381..16331
                     /locus_tag="ACG8WP_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9027597.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16324..17283
                     /locus_tag="ACG8WP_001284"
     CDS             16324..17283
                     /locus_tag="ACG8WP_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9027598.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17280..18035
                     /locus_tag="ACG8WP_001285"
     CDS             17280..18035
                     /locus_tag="ACG8WP_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027599.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18058..19011
                     /locus_tag="ACG8WP_001286"
     CDS             18058..19011
                     /locus_tag="ACG8WP_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9027600.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19534..19830)
                     /locus_tag="ACG8WP_001287"
     CDS             complement(19534..19830)
                     /locus_tag="ACG8WP_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9027601.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20185..20541
                     /locus_tag="ACG8WP_001288"
     CDS             20185..20541
                     /locus_tag="ACG8WP_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9027602.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20551..21450
                     /locus_tag="ACG8WP_001289"
     CDS             20551..21450
                     /locus_tag="ACG8WP_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9027603.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            21970..22698
                     /locus_tag="ACG8WP_001290"
     CDS             21970..22698
                     /locus_tag="ACG8WP_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9027604.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22843..24870
                     /locus_tag="ACG8WP_001291"
     CDS             22843..24870
                     /locus_tag="ACG8WP_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9027605.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            24978..26075
                     /locus_tag="ACG8WP_001292"
     CDS             24978..26075
                     /locus_tag="ACG8WP_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9027606.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26382..>26817
                     /locus_tag="ACG8WP_001293"
     CDS             26382..>26817
                     /locus_tag="ACG8WP_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027607.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         8996 a         4636 c         5667 g         7518 t
ORIGIN      
        1 aataattcat tacacaaaga taagaataac ctttgttata aaacgcttta ttctgtgtaa
       61 tgtccttttt tattaagaaa aacttccatt taggaagcag agaggggtat tcgattgtcc
      121 gataaccaat tattagaagt tcagaaccta catacaggat tccgactcaa agatgactat
      181 tacgatgcag tagatgacgt ttcatttact ttagacaaaa acgagatttt agcaatcgtt
      241 ggggaatctg ggtgcgggaa aagtaccttg gctactacga ttatgggatt acttgatcct
      301 aataacacaa aaattacagg tgagatttta tataacgatc tgaatttaat cgacctgaac
      361 gaaacgctct ataataaaat ccgcggaaat gatatcggaa tgattttcca agatccgttg
      421 tctgcattaa atcctctcat gagaatcgaa gaccagatca aagaaggact ttcctatcac
      481 acaaagatga atgcagaaca gcgtcaagct cgtgcactag aattgctaga acaagtaggg
      541 attcctaatc ctgcgagagt tggacgccaa tatccccatg aactatcagg cggtatgaga
      601 caacgtgtca tcatcgcaat cgcaatcgcc tgcaaacctc caatcgtcat tgcagatgaa
      661 ccgacgacag ccctagatgt aaccatccaa gcacagattc ttgatttact gaaagacctt
      721 caggaagaaa caaaaacagg gatcatcttg atcactcatg acttaggagt cgtagcagaa
      781 atggctgatc gagtggcagt gatgtatggc ggacagttcg ttgaagtagc aggtgtaaaa
      841 gaactgtttg atcatcctaa gcatccttat acacgctctt tactgaactc tataccacaa
      901 gaaggaaatc atgatgcaga actccatgtg atcgaaggtg tcgttccttc tctgaaaaac
      961 atgcctcgta aaggttgtcg gtttgcacca cgtattccat ggataccagc aagcgatcat
     1021 gaagaagagc ctatccttca tgaagtcgca ccgaaccact tggtacgttg ctcctgctat
     1081 aagcatttcc actttagaga cgaaaaagga gaggtgtaga tgacagaatt aatcacaatc
     1141 aaagacctga aagttcatta ccctattcgc agtggattct tcaatcgagt aacggatcat
     1201 gtatatgctg tagatggtgt ggattttatt atcgaaaaag gaaagacata tggtttagtg
     1261 ggagagtctg gctctggaaa atctactacg ggaaaagccg ttgtgggatt agaaaaggta
     1321 acagccggac aaatcatgta tgaaggaaaa gatgtaacga agcgaagcaa ccgcaaaaaa
     1381 atgggttata acaaggatgt gcagatgatc ttccaagatt caatgtccag tttgaatcct
     1441 aaaaagcgag tgttggatat cattgctgaa cccatccgaa attttgaacg gttaagcgat
     1501 caagaagaaa agaaaaaggt aaaaggactg ctagatatcg ttgggatgcc ggaagatgcg
     1561 ctatataaat atcctcatga attttcaggc ggacaacgtc aacgtctagg agtagcccgt
     1621 gctgttgcta ccaatccaaa actgatcgta gccgatgaac ctgtatctgc tctggatcta
     1681 tctgttcaag cgcaagtatt gaatttcatg aaacgaatcc agcaagaata cggcctcagc
     1741 tatttattca tttcacatga tttaggtgta gtaaaacaca tgtgcgacaa tatcgcaatt
     1801 atgtacaaag gtcgatttgt agaaatcggt acaagagaag acatttataa tgacccgcga
     1861 catatctata cgaaacgtct gctttcagct attccgcaaa tcgatgtaga aaatagggaa
     1921 agccacaaag aaaatcgacg aagagtagaa caagagtata tccaaaacca aaaagaatac
     1981 tatgatgcga ctggtcgagt ctacgatttg cgtacgatca caactacaca caaagtggcg
     2041 ttgaaagatg gaggtgcaag ctaatgtgga aaacgattct gcgacgtgtt ttattgatga
     2101 tcccgcaagt tattatttta agtgttctga tcttcttatt agcccaagcg atgccggggg
     2161 atccatttac cggtttgatc aatccaaatc aggatccggc agtgatcgaa cagatgcgac
     2221 aagcagctgg cttgaatgac ccatggtatg tccagtactt ccgttggatc ggaaatgctt
     2281 tgcaaggcga ttttggtcaa agtttcttat acaaacgctc tgttgctgac ttgatcggcg
     2341 aacggatcat caacacttta tatctatcga tcttgacagt tgtccttact tatttgattg
     2401 ctgtgcctct ggggatgctt gctggccggt atcaaaattc aatcttggat aagattgtcg
     2461 taatctacaa cttctttagt tttgctgttc ctttatttat cttcggcttg atcatgctat
     2521 ttgtttttgg ttatcgttta ggctggttcc caacaagtgg atcacaaaca ctaggtttgc
     2581 aaggtggatt tatcactcaa tggatagacc gtttgaaata tattctgtta ccatcaatta
     2641 cccaagcatt tttggcgact gccgtcacaa tccaatatct gcgaagtgaa gtaatcgatt
     2701 cgaaatcgct cgattttgtc agaacagccc gttccaaagg ggtaccgacg aataaagtat
     2761 tcagtcgaca tattttccga aatgcagcgt tgccgatggc ttctcaaatg gggtatgaaa
     2821 tcacttcgtt gattgctggt tctgtagtta tcgaaaagat ttttggttat ccgggagtcg
     2881 gaaaactatt tattgattcc atcggccaac gggactatac cgtgatcaca tccttagtat
     2941 tgattttagg aatcgcgacg ctagtgggga cattactttc agatatcatc atgagtatcg
     3001 ttgacccgcg tattcggatt caataaaacc aatcagaaag gagaatggat cgatgagtga
     3061 aaaaaacaat acagcagttc aagaaagtat tcctcctatg gggatacgaa tgattgctcg
     3121 agaatttaaa aaagataaaa ttgctatttt ttcattaggt ttgctagtgg ttttattatt
     3181 agtgatcttt atcggtgcga tgctgaccga tcaagacaaa gtgatgtatg ttagtatctt
     3241 cgacaaatac gctgcaccag gaacagtttc gacacaagga caaaagttca ttctgggagc
     3301 agatgaaggc ggacgtgatg tattaggaca attgatcata ggtgctagaa actctgttac
     3361 gatcggattt gcgatcacga tccttacttc gatcatcggg gttggattag ggatcatctc
     3421 tggctactac ggagggatca ttgacaacat cttgatgcgg atcgttgatt ttatcatgat
     3481 tctaccgatc atgctgatca ttatcgtctt cgtatcgatt atttccagat acaatgtctg
     3541 gtcctttata ttcatcatga gtgcatttta ttgggtagct aaagcccgat tattccgaag
     3601 caagacatta tctgaagcaa gaagagatta tgtcagtgcc tcaaaaacaa tgggaaccaa
     3661 tgattttaaa atcatgttcc gcgaaatcat gccgaatcta agttcattaa tcattactaa
     3721 tttgacgatg aactttgcgg caaacatcgg tatcgagaca actttgactt tcttaggttt
     3781 cggcttgccg gcaaatgtac ctagtttagg tacattgatc ggttatgcaa gtaacggaga
     3841 agtcttagtg aatcggcaat ggatctggtt acccgcatca atccttattt tagttttgat
     3901 gttgagtatc aattatgttg gacaagcatt caagcgttct gcagatgcgc gccaacggtt
     3961 aggctagtat agaagcgaag ggggaaatag gaatgaagaa aacgattttt ggatttgcag
     4021 cgctgatgtc tgctgtcgtt cttgcagcat gcgggggcgg aggtacagat acctctggaa
     4081 acaactctgg aggcaacggc aataacaaag aggaattaac ttttccaatt gcaacaaaaa
     4141 ataatgatga tgcaatcgaa gacggcgaat tagaagctgc ggtcgcaacg gacagtcagt
     4201 ttaaaggatt gttccagtgg gagttttacc aagattcata tgatgcagct tttatggctc
     4261 cttctcatga accgctattt gcaaacgatg acgatttcag aatcacagac gacggagctg
     4321 caacgcttga tctagatgaa gatacgaaaa aagcgacaat caaagtaaaa gacaacgtca
     4381 aatggtcaga tggtgaagat gtaacggcgg acgatgtgat tttcccttac gaaatcattg
     4441 ggaataaaga ttacactggt atccgttatg acgacacttt ccgtaacatc gtaggtatgg
     4501 aagaatatca tgatggaagt gccgacacaa tctccggaat caaaaaaata gacgacaaaa
     4561 cagttgaaat cgaatataaa gaagtaaacc caagcatgct tcaatcgggt ggtggtatct
     4621 ggtcatatgc agcaccaaaa catacgttag aaggtgtagc aatcaaagat atggaatcaa
     4681 gcgatcaagt acgtaaaaat ccagtgactt ttggccctta ttacatgagt aatatcgttg
     4741 caggagaatc agtagaatac ttgccaaacg agcactactg gaatggtacg cctaaattga
     4801 aaaagatcac gatgaaatct gtaccgatgg caagtgcaac tgaagcattg aactctaaat
     4861 tatatgatat gattttccaa atgccaacag atacttacga tacctataaa gaaattgctg
     4921 gctatacaaa cttaggccgt gaacaaactt cttatactta tctaggattc aagttaggta
     4981 aatggaatgc agatacaaag agtgtggaat acaatccaga ttcaaaaatg gctgataaat
     5041 cattacgtca agcaatgggc tatgcattag ataataatgc agtaggtgaa cgtttctatg
     5101 caggtttgag aagcaatgcg acttcattga ttcctccagt attccagtca taccatgatg
     5161 atgagatgaa aggattcaca caagatctag acaaagcgaa aaaactatta gatgatgctg
     5221 gctacaaaga tgtggatgac gatggtttaa gagaagacaa agatggaaat aaactaacaa
     5281 tcaactttgc ttcgatgtcc ggtggggaaa cagcgcaacc attggcagat tactatatcc
     5341 aacaatggaa agaaattggt ttggatgtcc aatacacaac aggtcgtctg atcgaattca
     5401 attctttcta tgataaatta gaaaacgatg atcctgaaat tgatgtttac caagctgctt
     5461 ggaatacagg aactgatcca aatccaagtg gactatgggg accaaatgct gcgttcaact
     5521 acactcgtta tgaatcagaa gaaaatacaa aattgatcaa cgatatcagt tcaagcaaag
     5581 catttgatga agagtaccaa aaagaagcat atcaaaaatg gcaacaatat gcatttgaag
     5641 aagcatttgc gattccaacg ttgttccgta atgaagtatt gccagtcaac gaccgtgtca
     5701 aagactggga ttggtcttat gatgtaccaa acccatgggc aaatgtttct gttacagcag
     5761 attcacgttc ataatcacta aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt
     5821 gatatttttc tgtttatttc tctaatattc aggtcaaggc gctgatttca tgacttctcc
     5881 aataaacggt gttcaaagtc cagtttctcg gtattaagtc aaatttatgc aaaaatatgg
     5941 aaagccattt tcacaaaatt ttttcttcat actcgaaact aaacgaattt ttcacaactt
     6001 ctcgtcaatc actaggatta ttaaagcttt acttaccttt tgaccgaacc atttcaaatt
     6061 tgacaaaaaa ccagtataat aaggaagtat cgaaaaaata tccataaaag gaatgtggaa
     6121 aatggacaac cagctgacaa aagaactaaa agaaaactat aacatcgttt ttcacgatct
     6181 aaatttgctt gaacaagctt ttacccattc atcctatgtg aatgagcatc gcaatctgca
     6241 attatcggat aatgagcgac ttgaattttt aggagatgcc gttttagaac tgatggtttc
     6301 tcagtattta ttccgtttat atcctcattt gccagaagga aaattgacaa aaacacgagc
     6361 tgcgatcgtt cgcgaagaca gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata
     6421 tgttttgttg ggcaagggag aagagaattc aggaggacgt acgcgaccag cattgctttg
     6481 cgacctattc gaagcttttt taggcgcgtt atatcttgac cagggattcg acacgaccca
     6541 tgcattcatc gaaaaagtga tttttcctaa agtcaaagca ggtgcttttt cacatgagat
     6601 ggatcataaa acaaaactac aagaagtctt gcaaaaatca ggtgatgtat caatcgaata
     6661 ccgattgatc aatgaagaag gtccagctca tgaacgagta ttttggatcg aagtttatgt
     6721 agatgatcaa ttgattggca ctggacaagg gaaatcgaaa aaattagcag aacaagctgc
     6781 ggcggaaaat gcattggcag cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac
     6841 gaattgaaat agccggtttt aaatcatttg cagacaagac agtcattgat tttgaaaata
     6901 gcgtcacagc agtggttgga ccaaatggca gcggaaaaag caatattaca gaggctatcc
     6961 gctgggtatt aggagaacaa tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca
     7021 tctttgccgg ttccgatacc cgtaagcagc tgaatattgc agaagtcaca gtgattttag
     7081 acaatagtga tcattatctg ccattggagt acaatgagat cagtgtaacc cgccgttatc
     7141 gacggactgg ggaaagcgag tttttcatca ataaacaaag ctgcagatta aaagatatcc
     7201 aagaattgtt cttagattct ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa
     7261 aagtagaagc gattttcagt agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag
     7321 ctggtgtctt gaaatacaag cagcggaaaa agaaagcaga acagaagctt tttgaaacag
     7381 aagacaacct cagccgcgtc caagacatca tccacgaatt ggaagaacag ttaactcctt
     7441 tggccgccca gagtgaggca gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa
     7501 ccgatgtctc tttgatggtt gctgaaatca agacagcaaa aaaagattgg gacaacaaac
     7561 aagcacagct tgcgaaattc aatctagaat taggcaaact atctgagtcg atccaagaac
     7621 aagaatccat tttggcgaag cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa
     7681 aaaatcagca agtattatta gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag
     7741 atgttttgca ggagcggaca aagcatacac aaaaaagctc gcaagaatat caaacttcat
     7801 tagcagaagc acaaaaaaag gttaaacatt ttgaaaagct tcaagaaagc ttgatgaaag
     7861 cagcagcaga aaaagaaaca gagatccaaa aagctgaagc aaatctcata aaaacacagc
     7921 aagaattaga aaaataccaa aaatcaacga aagaattatt ggcagaatta agagatcaat
     7981 atgttgattt gatgcaagaa caagcagctg taggaaacga actgaagtat ctagaacgtc
     8041 aatatataca agaaacagca aaaagcaagc agacgttagc caagcaatcg gaagtagaag
     8101 catcagtaga ccgattgatc ttacaaaaag aagaattgac acaaaagcag gcgcagctaa
     8161 aatcatcttt aactgaaaca aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt
     8221 tccaagaagc attagcaaaa gaacagccta aaatgtatca attaatgaat caagtccagc
     8281 agctacgtgc tcgtaagaaa agccttcaag aaatccaaga aaactatttt ggtttttacc
     8341 aaggggttcg attggtcttg cagcataaac agcagctttc gggtattgtc ggagcagtgg
     8401 ctgaactgat cgatgttcct gcagatttca cgttagctat cgaagcagct ttaggtgggg
     8461 cagcccagca tgtcatcgtt gaaaatgaaa aagatgctcg gcaagccatc acgtatttga
     8521 aacagcaaag aggaggaaga gcaacattcc ttccactgac gacaatcaaa ccaagacaat
     8581 taccggctca tatattgaca caagcagcag ctgtagaagg gtttataggg attgccagtg
     8641 aacaagtcag ttatcccgat caaatccaaa ctgtggttca aaacctgcta ggaacgatcc
     8701 ttttagccaa agacttaact agtgccaatg caatcgcgca aacgatccgc tatcaatatc
     8761 gagtggtttc cttagaaggt gatgtgatga atgccggagg atccatgaca ggtggagcga
     8821 ataaacgagg aaaccaaggc agtttgtttg tacaaaatca agaattgaaa cagttgacta
     8881 gtgaatttga agaagcagat aaacaactcc aagcacaaga aaaaaaagtt caggagctgc
     8941 aacaagaaac agctcgttta gccgaagaac aagaagtttt gcgtacacga ggcgaacaat
     9001 tacgttttga agaacaagaa gcgacaaatc agctacaaaa catcataaat gaacttgaac
     9061 gttttgaaaa agaaaagcaa atctcaactt ttgagacacg tgaactgcag cagtttatcg
     9121 aagattacga aaagcaaacg aacgaattga aagacaaaca aacagatctt gaatcgcaac
     9181 gacaaaaaat cgacgaagaa atcaaatcgt taagtcaaga aagcgaccag atggaagcca
     9241 gaagagcaca agtacaaagt caaaaagcac aagaacaagc agatcttgct gtcttgaaag
     9301 agcagtttaa tcatctgcag atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa
     9361 ccgaacgtca agaagcgata gaaaagcagt tggcgacatt gacagcagac ttttctgatc
     9421 atgaagtcac agaagaaagt ttggaaaaac aaattaacga attatctgct cagcgagaaa
     9481 cattaaaagc cgagttggca aaagccaaag agcagcgaga tagaaggcaa aaggagatcg
     9541 ataagcttga agcagtcttg gcagagcgta atcgtgagca aaaagcacga ctgtcggaac
     9601 aatccaaatt agaagtccaa aaagaccgag ctgaaatgat tttagataat catttgacct
     9661 atctgcagtc agaatatcag ctgacatttg agaaagcttc ccaagactat caagaaacaa
     9721 ccgatatcga agacagtaga acaaaggttt catccttaaa agaacagatt gaaaaactgg
     9781 gaccagtaaa cttaaattca atcgaacaat acgaacaagt cagtgaaaga catacattct
     9841 tagcaacaca gcgagatgat ctgttagcag cgaaaaacca attgtttgaa acgatggatg
     9901 agatggatga tgaagtgcgg acaagattca aagaagtatt tgaagctatc cgtcaagaat
     9961 tcaaaattgt ctttccaaat atgtttggcg gcggtaaagc tgaattagta ctgacggaac
    10021 caactgattt gctgaaaaca ggtattgaga ttgaagtaca acctcctggc aaaaaactgc
    10081 aaagtctaag tttgctttct ggaggagaac gagcacttac agccatcgcg ttgctctttt
    10141 ccattatccg tgtctgtcct gttccgtttt gtatcttaga tgaggtagaa gcagcgttag
    10201 atgaagccaa cgtgaaacgt tttggtcgtt acttgagcga tttccaagat gacacacaat
    10261 ttatcgtagt cacacaccgc aaaggaacga tggtcgcagc agatgtactg tacggagtga
    10321 cgatgcagga atccggtgta tcaaaaatcg tttctgtacg gatggaagat atcaatgaag
    10381 aggcgaaaat caacgaaacg aaagtgtagg aacgaaaatg atcaaattag tagcaatcga
    10441 tttggacggt acattattaa atgaacaaaa gctgatttca gatgagaata aacaagcatt
    10501 agcacaagca aaacagcaag gggtcaagat cgttctatgt acaggacgcc ccttagctgc
    10561 gatggcgcat tatctgcaag aattaggact ggtagatgaa ggtgactaca gcatcacttt
    10621 caacggcggt ttggtacaaa aaaatgatac tggtgaaatc atggaaaaga aagtgatgga
    10681 agtatcagat atccaacgct tatacaaact ggctcagcaa ttggatctgc cactagatgt
    10741 tttgtctgac aatgtggttt tacagctgcc tagtgcccca aggaagcaat ccttgtacaa
    10801 tgtgttgaat aagctgctgc aattccaacc agcggatata agcgatatta cagatgaatt
    10861 tgtgcttaat aaggcagtaa tcgcttatgg ccaagaagaa ttagatccta aaatcaaaga
    10921 aataccagca gaattccatg aaatgtatga gatcatcaaa acaagaagta tgcttttgga
    10981 atttatgcca aaaggtgtta caaaagctta tggtatttct ttattggcaa aagatcttgg
    11041 tttgaaacaa gaagaaatca tggcgatcgg tgatgaggaa aatgatttac cgatgatcca
    11101 atatgctgga ctaggcgtag cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga
    11161 tttcgtgaca gccagcaatg aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt
    11221 agagtagggg gagtcaaatg ggattttttg ataaaatcaa gcgtgctttt actggcgaag
    11281 agaaaacaga agaagttgaa gtacaggaga agtacgacaa gggtttagaa aagacgcgta
    11341 aaacatttgg tgaacggatg aatgagttgt ttgccaattt tcgaacagtc gatgaagact
    11401 tttttgaaga attagaagaa acacttatcg gtgcagatgt aggttttgaa acagcgatac
    11461 gaatcaccga agcattacgt caagaagtga aactcagaaa tgcgaaaaaa tcggcagaag
    11521 tccaaaatgc gatcatcgag aaacttgtgg atctctatga agcggaagga atcaatgaag
    11581 taaatgaact aaatatccaa gaagacggac taacagttat tttgtttgtc ggtgttaatg
    11641 gtgtagggaa aacaacaagt attggtaaac ttgcccatca gttcaaaaac gaaggtaaaa
    11701 aagtattgat ggcagcagca gataccttcc gcgctggagc aatcgaccag ttagttgtct
    11761 ggggcgaacg cgctggtgtt gaagttgttc gtggcaatgc aggaggcgat ccagcagctg
    11821 tcgtattcga tgctttggaa agagcaaaga cagagcaggc agatgtttta ctagttgata
    11881 ctgcaggccg actgcaaaat aaagtgaact tgatgaacga attagaaaaa ataaaacgag
    11941 taatccagcg ggaattacca gaagcacccc aagaaacact tcttgtatta gatgcaacca
    12001 ctggtcaaaa cgcgatggtt caagcaaaac aattcaaaga aacaacagat gtgaccggtc
    12061 ttgttttgac aaaattagat ggtacagcaa agggcggtat cgtacttgct atccgtaatg
    12121 aattgcattt accagtgaaa ttggtcggtc ttggtgaagg aatcgatgac ttggagccgt
    12181 ttgatccaaa tgattttgtc gttggattgt tcaaaggatt actaaaagaa gaataaccca
    12241 tgtctaaaaa gcaggagctg tgacaaaagt tgcagctcct tgtttgaatg attgttttta
    12301 acatagctgt gtttttgatg ccttcatttt tatacttatt tgtaagcggt tctcaaacaa
    12361 tttcgttctt tttcttagac tttttgatta taattagatt catatgaatt ggaggtcact
    12421 taatgatgaa ttttaagcaa ttattattgc atgtcaatgc gcgccctttt atcgaccaag
    12481 ctcgatttgg tatagaacgg gaaggacagc gggttgatct tgcaggaaat ctagcaaaaa
    12541 ccgatcatcc agcaatcttt ggcgatcgat cctatcaccc ctatatccaa acagatttta
    12601 gcgaaacaca aacagagatg atcacccctg ttaccgattc tattcccgaa ttatttcagt
    12661 atctcgctgc tgtttatgat gtgactgctc gttctatacc gaaagaagag atgatctggc
    12721 cattaagtat gccacctgcc ttaccggaaa aagacgaaga gatcattatt gcaaaattaa
    12781 aaaatttcga agatgtcttg tatcgacgtt atttagcaaa aaaatacggg aagcgaaaac
    12841 agatggtaag cggtattcat ttcaattttg aatttggcga tgagttgcta agaacattgt
    12901 tcagccatca ggaagaattt caagattttt ccgaattcaa aacagaactt tatttgaaaa
    12961 cagcgagaaa ctttatgcgc tatcgctgga tgatcactta tttattcggt gcttcaccaa
    13021 tgagtgagaa gaactacttt ttagatgaat cacatccgca agaacctgtt cgcagtattc
    13081 gcaacagtgc gctgggctat acgaatcatc caaatgtgaa agtttcctat gcttctatga
    13141 aacagtattt agcagatatc gagcgaatga tcgaagaagg gaaactttcg gaggagaaag
    13201 aattttatac accgcttcgt ttccgaggcg gaaagaaagt cgcagattta gcaacaacag
    13261 gtgttcgtta tattgaattg cggaatatcg atttgaatcc ttatgcaaga ttgggtatca
    13321 atccagagca agttcggttc ttacaattat tcctgatgta catgttatgg acagaagaaa
    13381 aagaagactg tgaccaatgg gtagcagaag gtacgactcg aaataacaaa gtagcactag
    13441 aacaaccatc agatcaaaca gaatttcatc aagaaggtag agaaattctt gaaggaatga
    13501 agcaaatgct ggttgaattg gattggttgg attctcttta tttagtagag gaagcattga
    13561 ctcaaatgga tcatcctgaa caaacgttag cagcgaaact ctatcaagaa gcacagctat
    13621 cgagtcagca agaagttgct gtggcattgg gacatcaata ttataaagaa agccatgaac
    13681 gtccctatca gttggccggt tttcgcgaaa tggaactttc tactcagata tttatgttcg
    13741 atgccatcca aaaaggtgtc caagtcaaag tattagatga gtcggatcaa tttttgcgtc
    13801 tgcaattcca agaccatgta gaatatgtga aaaatgcaaa catgacaagt aaggacagct
    13861 atatcgtccc acttatcatg gaaaacaaaa cagtaacgaa aaaagtctta aaagaagctg
    13921 ggttccgggt accaggaggc gcagaatttt catccatgga agaagcggta aaagcttatc
    13981 cgagatttgc cgagcaggca tttgtgatca aacctaaatc aaccaattac gggttaggta
    14041 tcacgatttt caaggaagga gctagtctgg aagattatca agcaggttta gccattgctt
    14101 ttcgtgaaga cagttcggtt ttagtagaag aatttatgcc gggaacggaa tatcgtttct
    14161 ttgtgatcga cggagaagtc caagccatca tgttgcgagt ccctgctaat gttattggtg
    14221 acagtatccg aacagtgaaa gaacttgttg aagaaaaaaa tagtgaccct ttgcgtggaa
    14281 cgaatcatcg tgcaccttta gaattgatcc aattaggtga attagaacaa ttgatgttga
    14341 aagaacaagg gttgacgatc gaatcagttc cccaagccaa tcaaattgtt tatctaaggg
    14401 agaactcaaa tatcagtaca ggtggtgatt cgatcgatat gactgatgaa ttttctgaag
    14461 cttacaaaaa aatcgccgtt tctgcggtag aagcactagg agctaagatt agtggtatcg
    14521 atttgattat accagataaa gaaatcgatc ctacaactga taaaaaagca tatgggatca
    14581 tcgaagcaaa tttcaatcca gccatgcata tgcatgttta tccttttgca ggaaaaggca
    14641 gaagattgac gatgaatgta ttgaaattgt tatatcctga agtattttaa aagaaaaagc
    14701 ttttgacaga ttgtcctatt tgatgtcaat ctgatcaaaa gctttttttg catgtaaaaa
    14761 tttatgtgtt ttttcgtttt aaaataaacg atcccagaag ctttttttct tttttggttt
    14821 ttctttttca gctggcgaag agttttcttc tgggtatttt ttttcgatat caatcacagg
    14881 ttcatttact gcttcttttg ctgttaagag caatccgaat tcctcaggat ttgcagtgac
    14941 gtaatcgttg actacagtga aacggctatt gtttttcgtt gctagctgga tatattggtt
    15001 ttggacagaa aggggtagag ccccattcaa gagtatggaa gcatctggat gttttttgag
    15061 ttcttttaaa aagttttctt tgttttttgg ttggatcatt tcagcaacag tcatactaag
    15121 atagcagcgt tccctgaatg tccctaaata tttatgctgt tcttcagggt tcactaaagg
    15181 tgtaccataa agtcccttat ccaaatggct ttttacatca tcttgtgcca taaaaaatac
    15241 cctccttctt ctctttgtct ttcttacgat tatagcatat tcccaataat acctaaaaca
    15301 tatgaaaaag cggttgcttt tgttcaacga ttcacttaaa ataagaatga aaatcattct
    15361 caattagaaa ggtggattgg atgcgctacg gacgactagt agcattatta gttattttaa
    15421 gtagtatttc attgttcatc ggagtatcga atgtacaatt gtcagaactg ctccagttag
    15481 atgggcaaag cttacaaatc ttgctagtga ctcgtttgcc acgaacgatt tgtttgatcc
    15541 ttgtgggagc gaccagtagt atttgtgggt tgatcatgca acatttgacc caaaataaat
    15601 ttgtttcacc aacgactgcg ggcacgatgg atagtgcgcg tttaggtatt ttagtggcta
    15661 tgattttcct acctggtgca tccttgattg tccgatcctt gacagcattt tgctttgctt
    15721 ttgcaggaac attgcttttt ttatccctta cgcgattgtt cctccagaaa aatcaattga
    15781 tgcttccgtt gattggagtc atgttaggga atatcattgg ttcagttgct actttttttg
    15841 cttatcaatt ccagttggta caaaatatga cttcatggct tcaagggaat tttgcgactg
    15901 tgatacgagg aaattatgag ttattgtatg ctgttgttcc attgttgctc ttggtttggc
    15961 tatttgctta ccgatttacg atcattggtc tgggcgaatc gtttgcagta aatctgggaa
    16021 tagattacca gaagatgcag tttttaggca ttggcttagt atcattggct agtgcagtca
    16081 tgttgatcat ggtaggaagc attcctttta tcggagtgat cattccgaat cttgtctcta
    16141 gaatatatgg agatcaagtg cacaaaacaa ttggcgtaac tgctttagca ggtagtttgt
    16201 tcttagtatt ttgcgatctt gtcgctcgcg tgttgatttt tccttatgaa ataccagtca
    16261 gtgtgatcgt tggaatcatc ggtggggcta tcttccttta tcttctgata aggggacgac
    16321 gatatgctta aaaaaaagcc atctattttt attggtatgc ttgttcttgt tttatttgtt
    16381 acaggaatgt acttgacgat ccatacatat ggaaactggc attttgcatg tgcattgagg
    16441 agtaagaaaa tttttgcatt tatccttgtc tcgctagctg tcagcagtgc cacaatcagc
    16501 tttcagacaa ttacgcataa ccagtttttg actccttcag tattgggact tgagtcgtta
    16561 tatgtccttg tccaaacggt ccttttcttc tttatcggag gagtgacgat gcttcagcag
    16621 acaggtacag tttttttctt attgaacgtt gggctgatgg tcgggctgag tctattgtta
    16681 tttttctttc ttttgaaaaa aatgagagcg aatctttttt tattactgat gtcagggatg
    16741 attttaggta cgctttttgg tagtgtcagt acctttttac aagtcatcat ggatccgaat
    16801 gaatatgatt tgcttcaagg cagattattt gcaagctttg gcaatatcca gagtcagtat
    16861 cttttgcctg cttctttgat cattatcggc tgctggtacc tgttatttcg atatcgacat
    16921 gtgctagacg ttttccatct cggaaatgaa caagcagtaa acctcgggat agacacagat
    16981 cgttttgaat ttttgatttt atgttgtgtc agtcttttga cagggacatc aacggctctt
    17041 gtgggtccag ttactttttt agggtttatt gtcgcaaata tcagttatcg tgtcttttct
    17101 acttatcgtc atggtcagct atttatcgga agctttctgg tagctatgct gtttctgaca
    17161 ggaggacagc tcgcagtaga gcagattttc ggctggaata caacagtgag tgtcgtcgtt
    17221 gaatttgtcg gaggaattta ttttgtcaca aaattgattc gcgaaaggaa gagtatccta
    17281 tgattgattt acaaaaggtc aataaactgt accatggaaa atcagtggtt aaagatgtac
    17341 agttaacgat tcctgaaaag aagttgactg cttttatcgg tccaaatggt gctggaaagt
    17401 caacggtgtt gtcgatgatc agtcgtttga taccaaaaga tacaggtgaa atctatttag
    17461 accataacga agtaaaggct tggaaatcca atgaattagc caaaaaatta tcgatcctac
    17521 gacaatcgaa tgaaataaat gtccaaatca ccgtgaaaga attagtagaa tttggacgat
    17581 ttccttatac gaaaggaaaa ttgacgacaa aagacgagga aatcgttcag gaatcactgg
    17641 aacaccttgg gttagaagaa atagcggatc aatcgattga cactctttca ggcggtcaac
    17701 tccaacgtgc gtatattgca atggtgctag cgcaagacac ggattatatt ttactggatg
    17761 aacctttgaa caatctagat atgaattttg cggttcaaat catgcagatt ttaaaaaatt
    17821 tagtacaaga aatcgggaaa acaattgtta ttgttcttca tgatatcaac tttgctgcaa
    17881 gttatgcaga cgaaattgtt gcaatgaaag atggacagtt atttacgcaa ggaacgaccc
    17941 aagaaatgat aaaaaaagat gtattggatg aattatacga aatggacata cggatttgtg
    18001 agattgaagg ccgcagattc tgtctttatt tctaaaacta acattaggag agaagagatg
    18061 aaaaaattag tagtaggtat cttgattcta gcatcatttg gtttagcagc atgtggaaat
    18121 agcaacaaca ccagtcaggc agacacaaaa aaaagcagca cacaaactga aactacattg
    18181 acgataacag atagtaatgg agaccaaatc gaggtgccaa ataatccaga aaaagtcgtc
    18241 gtattcgaca atggttcttt agatacgatg gatgctcttg gtgttggaga taaggtagtt
    18301 ggagcagcta caagcagcct tcctgagtat ctttcatcct ataaaaaagt agaatcagca
    18361 ggtggaataa aagaaccaga cttagaaaaa attaatcagc ttcagccaga tctaatcatc
    18421 atttctggcc gacaaagaga ttttcaaaaa gatctaagtc aaattgctcc gaccattttc
    18481 ctttctttag atgcgaaaaa tccatgggaa tctttccagc aaaatgtcac tgctttaggg
    18541 gagatctttg gtaagcaaga agaagctaaa actcaattag aggaattatc tagtgcaatc
    18601 gatcagacga aaaagaaagc agaagctact gacaaaaaag cactagtcac acttgtgaat
    18661 gaagggcaat tatcagccta cggatctggt tctagattcg gtttcatcca tgatttattt
    18721 ggctttgaac aagcagatga tcaaatcgaa gcatccactc acggtcaaag tgtttcttat
    18781 gaatatgtgc tggaaaaaaa tccggatatc ttatttgtag tagacagaac aaaagctatt
    18841 ggtggagacg acagcaaaga tgatatttct gccaatgaac tggtagcaca aacgaatgct
    18901 ggtaaaaatc agcagatcat ctcccttgaa ccagatgtct ggtatttaag cgggggcggt
    18961 ttagaatcaa tgaaactgat gatcgaagat gtgaatcaag catttaaata gcataaataa
    19021 cagaggatta aactctcata ctatgtaaca ataatgtttc ttacattctt aaatagcaag
    19081 agcctatcct caaagaagtt atcaaaaacc atccgtaaaa tcgtattgat gattttacgg
    19141 atggttttta tttatttgag aaatcttttt tcaacgtgaa ttcattatct acatcatacg
    19201 ggttgattct agaatgggga gtggcaaaaa atagtgtgat ccattttttc agcatcaaga
    19261 tcgcctcctt tgatttgaat caaaggtaca aagcgaaact tagataaaac ttaaatttag
    19321 atatttttat tttgtaccga tctcttcttt aaacgatgtt ccatctgctg cttctcggca
    19381 ataagtccac tagactagaa aatatgagaa gctattttct gtccagcgtt cttattactc
    19441 aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct gaccttcggt
    19501 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
    19561 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttag
    19621 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
    19681 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
    19741 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
    19801 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt cttttatttg
    19861 ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa ttctttttcc
    19921 aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag aaaggaataa
    19981 ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac tgaacgaaga
    20041 aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg acttactttc
    20101 caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa taaagtgatt
    20161 attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc acaagaagta
    20221 ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa cggtgacggt
    20281 ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt gatcattgtc
    20341 aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac accagttgga
    20401 ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac acttgcttta
    20461 gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg
    20521 caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat cagcgaccta
    20581 catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca agttttgcaa
    20641 aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt agaccgttgc
    20701 ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca tttcggcaat
    20761 cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga cccacatttt
    20821 ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat gaatggctgg
    20881 tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg
    20941 tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg
    21001 cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga ggtgatttta
    21061 tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc ttatgagcgt
    21121 tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact tgaccgttat
    21181 tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga taaatccatc
    21241 ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca gctgacccga
    21301 gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt acgtgtcctc
    21361 ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca agaggaattt
    21421 gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta aaagaaaata
    21481 ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga tatgctgctt
    21541 tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt
    21601 actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa tataatatac
    21661 attttttatt taattataat taatattgat tgaagtctag gcagtcaagt cacattttga
    21721 tagataggag ggggacttac gaattatttt aaaggctatg acagctgtat ttctagattg
    21781 tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg ggctgataat catttataac
    21841 taatcgatac ataggaccaa atataggtct atgttctttt atttcataag tatcgtaaaa
    21901 cagaattttg ctatggatta atccaatcga ctgatatgtt tagtaaataa agaaagagga
    21961 atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa tgatgttatt cagcatggta
    22021 gcaagtacga cttttgtttc tgcaactgaa gtatacccaa aggaatataa cacagaagga
    22081 acgattacat ttgaagcagg agatgagggt gtaactcctc ctgttgatcc agagaatcct
    22141 gatcccaata aacctgtcga tccatcagat cctccaagcc ctggaacagg aggggcatta
    22201 tcgattgatt atggatcaaa attcaaattt gggactcaaa aaatttctac tgcggataaa
    22261 acgtattatg cagctgctga tgtgatgaac gatgggtcta gaaaaccgac atatgtccaa
    22321 gtgacagata ggagaagtac cttatctggg tggaaattaa gtgtctctca accagaacag
    22381 tttaaaactg ccagtgggga tgaactagtt ggagcacagc tgaaatttac gaaagggcag
    22441 gctgtttctt tggttgatcc aacatatacg ccgcaaacgg tgaattctga attaacgttg
    22501 acaccgggag gaaacaatac gttagcaatt aatgctaaat caggaacagg tgtggggact
    22561 tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag atgcggtaca gctttcggta
    22621 cccggtaaat ctgtcaagtt agcgcaacaa tattccacta aattagtttg gacattagaa
    22681 gatacaccta acaactagtt tactgaaaag aactgttaaa aataaatacg tagtcatatt
    22741 gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg ctaaatttgt ttggatacga
    22801 atagttcatc taggaattta acgatcagga ggaaaagata tattgaaaaa gaggaggatc
    22861 gtgctcattt taagcagtct cttagtgact aatatctttc cgatcatagg gtactctttg
    22921 gaattgaatc agttggagca tggaaataga agagacgaag aaagcaaact cacttcgaca
    22981 ttcgacagtt taatagaatc caaccagaca caagaaagca gtgaggattt aagtatttcg
    23041 agtagtggag agaccactga caacgtagaa gctgagggtt cggacaaaca aggactagaa
    23101 gatgcaccaa tttctgcggt tgagctgcct gacgatccca atattatttc tatcgataaa
    23161 atttttcaaa cacctatcgg tactagcacg tccatattgg aaaatgggaa gttactgcag
    23221 ttgaacccag cagaaaggtc tcaaagagga gctatttggt caaataagaa gattagttta
    23281 ctctcggatt ttacatttaa atcttattta tacttaggaa atcaacgaga tagagcggga
    23341 gatggcatga cctttacatt gactggcgat cctagaatgg ctagtaatcc cagtcaagtt
    23401 attggatcgc caggtatggg aattggctca tattctactc gttctggcca accttacatt
    23461 gcgaatgcgc tctcgattga gtttgatacg tataaaaata gtggttcttc tgatcgaatg
    23521 gatcgagaga taactgctga taatagaggg catggacatg tcgcttttgt cacgcctaaa
    23581 ccaaataata ataattattc tggagagcat actggcgtga cggtagctcc tacatttctt
    23641 tctaatggca catggcgctt gttaataatt aaatgggacg cggagcaaag aagtcttact
    23701 tatgatttgg aaggtgtcgg tcgctcttct cggattatac ctgatctcat taggcagttt
    23761 ggttctacag aagtaaattg ggggtttact tcttcaacag gtactttttt tcaagaaaat
    23821 gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa cagctgatgt aaaagtcaac
    23881 gatagtgatt atggggccat cgctgaagct ggaggaaacg ataagataac tatcagaaat
    23941 acattgaagg tcgaggggga ttttcttgaa tataagaaaa tgcagactag tatcgacctc
    24001 ccacaagaat taactattcc cgagcatatc ttgatcaatg gaaatcaaat tgatgccaaa
    24061 gatatcttga taaccggaaa tcgagtcacg ctaaatctga cagattattt tattgctgga
    24121 gatacgttaa taatcgaatt ggagacaact ttgcaggaag taattcctga aaaagtactc
    24181 actatgcatt ttgaatacct tgaaaacggg aatttgattg agcaatcgaa tgaaataaac
    24241 ctaaagatcg caaagctgaa ggagatagat atccatgttt attataaaga taaagacagt
    24301 cagattgata ttgctgaaat gaaaacaatc actgggaaaa taggagagaa gtataaagaa
    24361 actcctattg atattgaagg gtatgttttt gactcggatt cagcgaatac agaaggcatc
    24421 ttctccgaaa atacagagga tatctatttt tattacagaa aaggcaaact ttatctctca
    24481 actgctcctc aatatgtcaa ttttggaaaa cataaaatat ccagtagacc attaagtgaa
    24541 ttcggacagt tttcaggtgg gttgaaaatt atagatgaac gggcttttgg aagttggaaa
    24601 ttacaattga aacaagagaa agttcttaca aatggagatt ttgagcttcc agaagctctt
    24661 tcctttgtta cctctgaaaa tacaacggtt atagggacat ccgctgttac gattttcgaa
    24721 agtgatcaaa agggtgaaag cgatttgtcc tctcttttag attctacagc tcatcgtgga
    24781 atcagaataa atattcctgt agaaaaccaa agaatcggaa cctttgaggg gaaactatct
    24841 tggatacttg cagatgtccc aggaaattaa caggaagaga agaatataac aaaattattg
    24901 agtttcaaga agggcacagg taccataggc tcttgtgtcc cacagagaca agattttgtt
    24961 atagtcttct cttacatatg attaaaggag cgattttctt gagaccaaaa caacaattat
    25021 ttataacact cctagttatt ttaggggcac tttttaacaa tatatcagtg tcagctagtg
    25081 catttaattt ttcagtgacg ccgataactt cagagaatca aatcgacaag agaaaaactt
    25141 acttcgatct tcaacttgat ccggatcagg aagtagaagt aaaagcggaa ttgagaaatg
    25201 atacagaaaa agaagtgaaa atcgatattt ctgtgaatag cgcaaccacc aattcaaatg
    25261 tgatggttga atacggaaaa aacgagattg aaaaagacat gagcctgatc tttgatctgg
    25321 tagattatgt gagctatcca cagatagtga cgctaaaacc taaaagtgtc caaaccgttg
    25381 cttttcatgt caggatgccg aatgagcgat ttgatggtgt attggctggt gggatcacat
    25441 tccaagaaat ccagacggaa aaggaccaaa cagaaactaa ggatcaaagc ttatcgattg
    25501 agaatgaata cacctatatt attgctttgt tgatgcaaca gacactgaac gaagttgctc
    25561 cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa tgcaagaaat gtcatcttag
    25621 caaacgttca aaatgatcaa aaaacctata tcaatcaagt agttattgaa acaaaaatta
    25681 caaaaaaagg acattctgaa gttttatatc aagaggaaaa agaaggattg cagattgcgc
    25741 cgaatacaaa tttttctttt ccaactgctt taaatggtca accgctaacc ccaggtgaat
    25801 atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa tggaaaattt tcgagaaaaa
    25861 aaggaaatac tacgatcaac tataccaatc aatgggtatt tgaaaaagat tttacaatag
    25921 acggaaaagt agccaaggaa ttgaacacga cggatgttac gattaaaaaa gacaataatt
    25981 ggatttatat cttagtagct tcacttttct tattgtttat tttattatgc atacttttgt
    26041 ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta agataacaaa tagtaaattt
    26101 ttattattgt aggatggttg ggctcttttt ataacaatat aaaatatacc attttattaa
    26161 taaaaatgca tactagtaaa tgatataatt ctaataacag gtttgatcgc gagaaaggcc
    26221 aaataaaacg ctctccaaaa tgttaaaaaa aagacaggac gatgtatatt atacaaacaa
    26281 gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg
    26341 aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt
    26401 taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt
    26461 tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa
    26521 aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag
    26581 taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc
    26641 tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg
    26701 aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt
    26761 ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt tttgaaa
//
LOCUS       DAZKAY010000033        26370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000033 DAZKAY010000000
VERSION     DAZKAY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.033"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACG8WP_001294"
     CDS             complement(243..611)
                     /locus_tag="ACG8WP_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9027608.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACG8WP_001295"
     CDS             761..1243
                     /locus_tag="ACG8WP_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9027609.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACG8WP_001296"
     CDS             1280..2290
                     /locus_tag="ACG8WP_001296"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9027610.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="ACG8WP_001297"
     CDS             complement(2405..3118)
                     /locus_tag="ACG8WP_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027611.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3291..3898)
                     /locus_tag="ACG8WP_001298"
                     /pseudo
     CDS             complement(3291..3898)
                     /locus_tag="ACG8WP_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4382..4609
                     /locus_tag="ACG8WP_001299"
     CDS             4382..4609
                     /locus_tag="ACG8WP_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9027612.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4625..5791
                     /gene="hemW"
                     /locus_tag="ACG8WP_001300"
     CDS             4625..5791
                     /gene="hemW"
                     /locus_tag="ACG8WP_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9027613.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG8WP_001301"
     CDS             6025..7068
                     /gene="hrcA"
                     /locus_tag="ACG8WP_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9027614.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7162..7725
                     /gene="grpE"
                     /locus_tag="ACG8WP_001302"
     CDS             7162..7725
                     /gene="grpE"
                     /locus_tag="ACG8WP_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9027615.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG8WP_001303"
     CDS             7776..9605
                     /gene="dnaK"
                     /locus_tag="ACG8WP_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9027616.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG8WP_001304"
     CDS             9756..10922
                     /gene="dnaJ"
                     /locus_tag="ACG8WP_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9027617.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLKYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11004..11633)
                     /locus_tag="ACG8WP_001305"
     CDS             complement(11004..11633)
                     /locus_tag="ACG8WP_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027618.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11645..11818
                     /locus_tag="ACG8WP_001306"
     CDS             11645..11818
                     /locus_tag="ACG8WP_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027619.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11863..12600)
                     /locus_tag="ACG8WP_001307"
     CDS             complement(11863..12600)
                     /locus_tag="ACG8WP_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9027620.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12602..14104)
                     /locus_tag="ACG8WP_001308"
     CDS             complement(12602..14104)
                     /locus_tag="ACG8WP_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9027621.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG8WP_001309"
     CDS             14363..16357
                     /gene="uvrB"
                     /locus_tag="ACG8WP_001309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9027622.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG8WP_001310"
     CDS             16367..19183
                     /gene="uvrA"
                     /locus_tag="ACG8WP_001310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9027623.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19446..20711
                     /locus_tag="ACG8WP_001311"
     CDS             19446..20711
                     /locus_tag="ACG8WP_001311"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9027624.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20715..21446
                     /locus_tag="ACG8WP_001312"
     CDS             20715..21446
                     /locus_tag="ACG8WP_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9027625.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG8WP_001313"
     CDS             21581..22465
                     /gene="rapZ"
                     /locus_tag="ACG8WP_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9027626.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG8WP_001314"
     CDS             22462..23460
                     /gene="yvcK"
                     /locus_tag="ACG8WP_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9027627.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23494..24429
                     /gene="whiA"
                     /locus_tag="ACG8WP_001315"
     CDS             23494..24429
                     /gene="whiA"
                     /locus_tag="ACG8WP_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9027628.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24972..25622
                     /locus_tag="ACG8WP_001316"
     CDS             24972..25622
                     /locus_tag="ACG8WP_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027629.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25809..>26370
                     /locus_tag="ACG8WP_001317"
     CDS             25809..>26370
                     /locus_tag="ACG8WP_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP9027630.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8709 a         4449 c         5602 g         7610 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
     1921 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
     2341 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
     2401 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
     2461 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
     3241 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
     3301 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
     3361 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
     3421 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
     3481 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
     3541 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
     3601 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
     3661 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
     3721 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
     3781 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
     3841 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
     3901 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
     3961 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
     4021 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
     4081 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
     4141 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
     4201 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
     4261 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
     4321 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
     4381 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
     4441 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
     4501 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
     4561 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
     4621 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
     4681 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
     4741 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
     4801 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
     4861 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
     4921 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
     4981 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
     5041 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
     5101 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
     5161 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
     5221 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
     5281 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
     5341 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
     5401 cctcatgtac tggaacaatg cccactattt tgggtttgga gctggtgcta gcggttatct
     5461 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
     5521 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
     5581 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
     5641 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
     5701 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
     5761 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
     5821 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
     5881 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
     5941 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
     6001 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
     6061 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
     6121 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
     6181 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
     6241 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
     6301 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
     6361 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
     6421 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
     6481 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
     6541 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
     6601 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
     6661 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
     6721 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
     6781 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
     6841 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
     6901 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
     6961 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
     7021 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
     7081 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
     7141 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
     7201 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
     7261 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
     7321 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
     7381 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
     7441 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
     7501 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
     7561 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
     7621 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
     7681 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
     7741 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
     7801 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
     7861 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
     7921 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
     7981 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
     8041 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
     8101 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
     8161 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
     8221 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
     8281 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
     8341 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
     8401 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
     8461 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
     8521 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
     8581 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
     8641 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
     8701 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
     8761 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
     8821 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
     8881 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
     8941 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
     9001 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
     9061 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
     9121 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
     9181 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
     9241 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
     9301 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
     9361 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
     9421 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
     9481 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
     9541 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
     9601 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
     9661 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
     9721 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
     9781 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
     9841 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
     9901 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
     9961 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    10021 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    10081 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgaaat
    10141 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    10201 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    10261 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    10321 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    10381 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    10441 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    10501 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    10561 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    10621 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    10681 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    10741 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    10801 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    10861 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    10921 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    10981 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    11041 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    11101 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    11161 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    11221 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    11281 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    11341 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    11401 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    11461 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    11521 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    11581 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    11641 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    11701 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    11761 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    11821 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    11881 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    11941 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    12001 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    12061 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    12121 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    12181 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    12241 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    12301 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    12361 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    12421 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    12481 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    12541 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    12601 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    12661 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    12721 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    12781 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    12841 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    12901 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    12961 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    13021 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    13081 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    13141 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    13201 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    13261 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    13321 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    13381 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    13441 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    13501 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    13561 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    13621 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    13681 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    13741 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    13801 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    13861 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    13921 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    13981 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    14041 acgcaagtgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    14101 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    14161 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    14221 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    14281 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    14341 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    14401 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    14461 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    14521 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    14581 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    14641 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    14701 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    14761 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    14821 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    14881 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    14941 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    15001 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    15061 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    15121 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    15181 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    15241 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    15301 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    15361 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    15421 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    15481 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    15541 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    15601 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    15661 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    15721 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    15781 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    15841 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    15901 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    15961 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    16021 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    16081 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    16141 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    16201 agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa
    16261 attagaaaaa gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt
    16321 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    16381 aattacgatt catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    16441 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    16501 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    16561 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    16621 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    16681 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    16741 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    16801 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    16861 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    16921 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    16981 catcgaccgg atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc
    17041 ggccctgcga ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    17101 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    17161 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    17221 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    17281 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    17341 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    17401 gatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    17461 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    17521 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    17581 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    17641 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    17701 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    17761 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    17821 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    17881 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    17941 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    18001 tgatgaagat acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga
    18061 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    18121 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    18181 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    18241 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    18301 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    18361 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    18421 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    18481 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    18541 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    18601 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    18661 taaacgatat aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    18721 tttggaaatg acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg
    18781 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    18841 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    18901 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    18961 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    19021 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    19081 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    19141 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    19201 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    19261 gaatcattac atggtagttc tttctacact gattggtata gataaaaact tcctataagt
    19321 tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt
    19381 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    19441 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    19501 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    19561 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    19621 caggatttga taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat
    19681 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    19741 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    19801 tatttgaaag gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    19861 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    19921 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    19981 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    20041 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    20101 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    20161 ggatgatgtg ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt
    20221 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    20281 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    20341 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    20401 acggcttgaa tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg
    20461 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    20521 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    20581 aagaaaatcg ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt
    20641 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    20701 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    20761 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    20821 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    20881 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    20941 gaccaaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag
    21001 cggcaacgga tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc
    21061 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    21121 atgaacgcga agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag
    21181 aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    21241 tctattacga aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag
    21301 gttgcacaga attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg
    21361 atgctcaatc tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg
    21421 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat
    21481 atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg cccgggatga
    21541 tataatttta ttatgcacaa tgtgagaggg gttcgtaaat atgacagata atttgcaatt
    21601 agtcatcata acaggtatga gtggtgcagg gaaaacagta gctatccaaa gttttgaaga
    21661 tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt
    21721 gatcaaagaa tcaggtaaag tcacaaaaat ggctttagga gtggatttgc gttctcgttc
    21781 cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac
    21841 ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag
    21901 acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc
    21961 gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacaa cattgacacc
    22021 acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg
    22081 gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat
    22141 ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa
    22201 agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa
    22261 atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt
    22321 gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt
    22381 ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag
    22441 gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc
    22501 gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttacg aaatcaagga
    22561 gcagatatca cagcagtagt gactgttgca gatgatggtg gaagcagcgg tgcaatcaga
    22621 gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat
    22681 atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta
    22741 gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact
    22801 tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct
    22861 tcagaaactc ctttgacctt gcatgctgtt tttaaagacg gaacgactgc agtaggagaa
    22921 tctaaaatcg cgatcaaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat
    22981 aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc
    23041 gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc
    23101 ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa
    23161 aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg
    23221 aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg
    23281 gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga
    23341 aatgaaggat gtcgagtgat ttccaccgac tttttagaat tgaaagatgg tggtgtcttc
    23401 cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa
    23461 gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa
    23521 gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg
    23581 cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa
    23641 aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt
    23701 gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg
    23761 aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat
    23821 ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt
    23881 gcttttatgg cctcaggttc agtcaataat ccggaaacga gtcgttatca tttggaaata
    23941 ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta
    24001 catgctcgaa cgcttgaacg gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa
    24061 attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt
    24121 cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat
    24181 ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca
    24241 aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa
    24301 catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc aatatcaaaa
    24361 tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa
    24421 gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt
    24481 ttttaaaagc tatacttgct tttcttccgt caaactataa ttccaacttt actggagtaa
    24541 cataatgttg aaaggcttct attgtttttt aatacaatcg tataaaaagc ggggagacaa
    24601 ctgaatgaag attttagttt tatacataaa cttttgctta atatagaagt cctgatatag
    24661 ataggatcaa gtatttatct ataactggag attaattaaa aactggaagg aactgctcga
    24721 tcaaccttga gaactgtttt tcttacttct ggtcaagatt ctcgtggttg gtggataagg
    24781 atggttgata agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa
    24841 gttttaacaa ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat
    24901 tgggatgtat ggttggtata cgttatacct gaacttgaac tatcaagatt tggtttagaa
    24961 tttgagaatc gatgtttata aagggagggc aaaaaatgtg cttttttaca acttttacta
    25021 tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt
    25081 tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt
    25141 ttctattcac agttattgta tttatttgga gaactacagc accaccattg ccagagatta
    25201 catatggaga atttccgttt agtcttgaat atgaaataga tgaggaaaga attttgattg
    25261 aagatacggt gattgcagaa tttataggaa gttttagagg gaatgtgaca actcaaccta
    25321 gacgaagttg gagtataaaa cttttaaata atagtggaga gttgaaagca tttcattatg
    25381 aattcttaat aatagaaagt gaagatgtaa caatcacttt aagtagtggc tctgctggat
    25441 attttatgaa tgatggtgga agcaagccac agctcacagt ccgtgatcgt attgcaggtt
    25501 ttatgcattt ttctcactat gacttagaag aagctcgtaa aatacttgca gaatatggga
    25561 tcacattaat caattgggaa tatgcgccac cgataagaaa ttttttcaat gattattatt
    25621 agtcttataa aaaacagaat gtaaaattta tgtgcgcgag tatttagttt tccatttttt
    25681 gtttgataga tatatagcat tgttgaagca tgggatagtg gtgccaattt tatcaatgag
    25741 gttggtcagg cagttaacat aattggtgat gctataaatc catttaattg gaattggagg
    25801 aattaggtgt ggaaacagtg agattatgga tattcatgaa aaattttcgt tcggctttga
    25861 gatatgttat tctggaatca aaatcaggtt attatctgat tgacacagat cactatactt
    25921 tttggggagg ctaccttttt ccgattttta attggataat cccccataag gttatgaaaa
    25981 ttaacttgcc tgcagaagaa ataaatagat taatactcaa caaagattta caaaggaaaa
    26041 gagagaaaga acattctaat gttgcttgga taggtccagc atttgcagta gtgacttctt
    26101 caattgtcgg tcctatgcta gagagtattt atagtgattt ttctattgga atacgttatt
    26161 tatttgttac tttaagtata gctgttcttt tgtgcataaa gggttatgtt agcaagaaga
    26221 ataaagatat tttaacacta atcggtgtaa acaatttatc tagtactaaa attatactgt
    26281 ttcctgaatc tataaaagaa ggatcaaggt tgttgataat ttttataatg cttatactgt
    26341 tttctgtttt atttggggtg atgtttatag
//
LOCUS       DAZKAY010000034        26160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000034 DAZKAY010000000
VERSION     DAZKAY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.034"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8WP_001318"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8WP_001318"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9027631.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACG8WP_001319"
     CDS             1286..2461
                     /locus_tag="ACG8WP_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9027632.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACG8WP_001320"
     CDS             2465..3106
                     /locus_tag="ACG8WP_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027633.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACG8WP_001321"
     CDS             3103..4020
                     /locus_tag="ACG8WP_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9027634.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACG8WP_001322"
     CDS             4017..4688
                     /locus_tag="ACG8WP_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9027635.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACG8WP_001323"
     CDS             complement(5229..5717)
                     /locus_tag="ACG8WP_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9027636.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACG8WP_001324"
     CDS             complement(5918..6184)
                     /locus_tag="ACG8WP_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9027637.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACG8WP_001325"
     CDS             complement(6184..6447)
                     /locus_tag="ACG8WP_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9027638.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACG8WP_001326"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACG8WP_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACG8WP_001327"
     CDS             complement(7250..9073)
                     /locus_tag="ACG8WP_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9027639.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACG8WP_001328"
     CDS             complement(9671..12376)
                     /locus_tag="ACG8WP_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9027640.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACG8WP_001329"
     CDS             complement(12682..13629)
                     /locus_tag="ACG8WP_001329"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9027641.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8WP_001330"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8WP_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9027642.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACG8WP_001331"
     CDS             complement(14264..14626)
                     /locus_tag="ACG8WP_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9027643.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8WP_001332"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8WP_001332"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9027644.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8WP_001333"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8WP_001333"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9027645.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACG8WP_001334"
     CDS             complement(16254..17765)
                     /locus_tag="ACG8WP_001334"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9027646.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8WP_001335"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8WP_001335"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9027647.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACG8WP_001336"
     CDS             complement(19592..20668)
                     /locus_tag="ACG8WP_001336"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9027648.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACG8WP_001337"
     CDS             20864..22609
                     /locus_tag="ACG8WP_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9027649.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACG8WP_001338"
     CDS             complement(22889..23326)
                     /locus_tag="ACG8WP_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9027650.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACG8WP_001339"
     CDS             complement(23310..24683)
                     /locus_tag="ACG8WP_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027651.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACG8WP_001340"
     CDS             complement(25005..25493)
                     /locus_tag="ACG8WP_001340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9027652.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACG8WP_001341"
     CDS             complement(25515..26117)
                     /locus_tag="ACG8WP_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9027653.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZKAY010000035        26101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000035 DAZKAY010000000
VERSION     DAZKAY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.035"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1168)
                     /locus_tag="ACG8WP_001342"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="ACG8WP_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9027654.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1572..2354)
                     /locus_tag="ACG8WP_001343"
     CDS             complement(1572..2354)
                     /locus_tag="ACG8WP_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027655.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2419..3255)
                     /locus_tag="ACG8WP_001344"
     CDS             complement(2419..3255)
                     /locus_tag="ACG8WP_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9027656.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3273..4682)
                     /locus_tag="ACG8WP_001345"
     CDS             complement(3273..4682)
                     /locus_tag="ACG8WP_001345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9027657.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4696..6519)
                     /locus_tag="ACG8WP_001346"
     CDS             complement(4696..6519)
                     /locus_tag="ACG8WP_001346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9027658.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7362..7688)
                     /locus_tag="ACG8WP_001347"
     CDS             complement(7362..7688)
                     /locus_tag="ACG8WP_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027659.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7734..8096)
                     /locus_tag="ACG8WP_001348"
     CDS             complement(7734..8096)
                     /locus_tag="ACG8WP_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9027660.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8133..8347)
                     /locus_tag="ACG8WP_001349"
                     /pseudo
     CDS             complement(8133..8347)
                     /locus_tag="ACG8WP_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8475..8810
                     /locus_tag="ACG8WP_001350"
     CDS             8475..8810
                     /locus_tag="ACG8WP_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9027661.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8886..10883)
                     /locus_tag="ACG8WP_001351"
     CDS             complement(8886..10883)
                     /locus_tag="ACG8WP_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027662.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10981..11256)
                     /locus_tag="ACG8WP_001352"
     CDS             complement(10981..11256)
                     /locus_tag="ACG8WP_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027663.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11488..12222
                     /locus_tag="ACG8WP_001353"
     CDS             11488..12222
                     /locus_tag="ACG8WP_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9027664.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12261..13403
                     /locus_tag="ACG8WP_001354"
     CDS             12261..13403
                     /locus_tag="ACG8WP_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9027665.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13498..14889)
                     /gene="sufB"
                     /locus_tag="ACG8WP_001355"
     CDS             complement(13498..14889)
                     /gene="sufB"
                     /locus_tag="ACG8WP_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9027666.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14894..15364)
                     /gene="sufU"
                     /locus_tag="ACG8WP_001356"
     CDS             complement(14894..15364)
                     /gene="sufU"
                     /locus_tag="ACG8WP_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9027667.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15351..16586)
                     /locus_tag="ACG8WP_001357"
     CDS             complement(15351..16586)
                     /locus_tag="ACG8WP_001357"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9027668.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16583..17869)
                     /gene="sufD"
                     /locus_tag="ACG8WP_001358"
     CDS             complement(16583..17869)
                     /gene="sufD"
                     /locus_tag="ACG8WP_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9027669.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17882..18655)
                     /gene="sufC"
                     /locus_tag="ACG8WP_001359"
     CDS             complement(17882..18655)
                     /gene="sufC"
                     /locus_tag="ACG8WP_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9027670.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18852..19700)
                     /locus_tag="ACG8WP_001360"
     CDS             complement(18852..19700)
                     /locus_tag="ACG8WP_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9027671.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19724..20410)
                     /locus_tag="ACG8WP_001361"
     CDS             complement(19724..20410)
                     /locus_tag="ACG8WP_001361"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9027672.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20403..21437)
                     /locus_tag="ACG8WP_001362"
     CDS             complement(20403..21437)
                     /locus_tag="ACG8WP_001362"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9027673.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(21500..21756)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21832..22170)
                     /locus_tag="ACG8WP_001363"
     CDS             complement(21832..22170)
                     /locus_tag="ACG8WP_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9027674.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22172..22528)
                     /locus_tag="ACG8WP_001364"
     CDS             complement(22172..22528)
                     /locus_tag="ACG8WP_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9027675.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22534..23721)
                     /locus_tag="ACG8WP_001365"
     CDS             complement(22534..23721)
                     /locus_tag="ACG8WP_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9027676.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23912..24757)
                     /locus_tag="ACG8WP_001366"
     CDS             complement(23912..24757)
                     /locus_tag="ACG8WP_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9027677.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24991..25956
                     /locus_tag="ACG8WP_001367"
     CDS             24991..25956
                     /locus_tag="ACG8WP_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9027678.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7618 a         5443 c         4424 g         8616 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccaagt tatctttaga
     1261 accatttcag atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt
     1321 ctagtagtat aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa
     1381 cagtctaaaa cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac
     1441 aaataaatat agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc
     1501 tactggagat gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga
     1561 tggaagaatc attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg
     1621 ttcttaaata ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct
     1681 ttaccaactt gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat
     1741 aaagattgag catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca
     1801 tttacaagct tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt
     1861 tcatctatcg tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt
     1921 tcatccatcg tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc
     1981 acacccaaac ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta
     2041 ctaataaatt cactataacc agttctttca atagagactt caatgtattt cacactatca
     2101 tactcaaaag aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc
     2161 tttgactcta cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata
     2221 ccagcatcct tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt
     2281 tcttgtgaac actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta
     2341 gaagaatttt ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc
     2401 agccgatatt ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc
     2461 gtcctgattt gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc
     2521 ccgatactct ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg
     2581 caactcttcc ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa
     2641 gtaaagcatc gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa
     2701 gatatccttt agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg
     2761 ggcatttaca atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat
     2821 ttttttgttg atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt
     2881 ttgaatttca atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc
     2941 atgcttcaag tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt
     3001 ggcgttggca taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc
     3061 aaacagttta atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt
     3121 ttggattttg tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc
     3181 catcaaaatt acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt
     3241 tttaattact ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact
     3301 ttttgatacc aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg
     3361 gttcggtcaa catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca
     3421 tcaatcgctg accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga
     3481 atagccagaa tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct
     3541 tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag
     3601 cgatcgtaca atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat
     3661 ggcgttttat ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg
     3721 atctgttgat cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg
     3781 gtggcagttg aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc
     3841 tgatcctctg cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa
     3901 taattgtatt ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa
     3961 ttatgggctg caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg
     4021 attgctggac cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct
     4081 tgagctaata aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca
     4141 cttggtggca aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc
     4201 agccaatagt tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa
     4261 taatcgatag ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg
     4321 aaatggtaca tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga
     4381 tgataaaaag aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc
     4441 caagcgatgg aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct
     4501 ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga
     4561 tacttgtcaa taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag
     4621 gcactaatgg aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc
     4681 acaaaaatcc tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct
     4741 tgcttcacta attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc
     4801 gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac
     4861 ctgatctcct gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat
     4921 ccccacatgg atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga
     4981 ttctaaaata gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg
     5041 aatggctacg ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat
     5101 aggacacacg gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg
     5161 ctctgattca tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac
     5221 aaaggcgatt acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa
     5281 ggctggtaag gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc
     5341 agcatacaaa ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt
     5401 caaggttact ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga
     5461 cacagctagc tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc
     5521 tgattgagca atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact
     5581 gctcaattgc atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa
     5641 tggctgcaaa ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc
     5701 agcccccatt gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc
     5761 taaaggcccc ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg
     5821 cgaaattcga tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg
     5881 taaaactgat cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc
     5941 gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc
     6001 caaaattggg ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa
     6061 ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag
     6121 tacaccagtc aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc
     6181 tggaatcatc ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc
     6241 ttgattgtct gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt
     6301 gaaggctgtt tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac
     6361 actaattaca ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag
     6421 ttcaaaacgc agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac
     6481 ctgttgcaat acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag
     6541 taaaaaagac caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc
     6601 ttgcctgcat aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac
     6661 attatgatag cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg
     6721 gtaatataac agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat
     6781 aagtatatct aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc
     6841 ccaaaatcgt gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc
     6901 tcttgggaaa aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat
     6961 ataggcaaca atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc
     7021 aatcattccg agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta
     7081 actagcaaat gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca
     7141 ccaattatca ataaaaatac caaacatatc cataataatt ttttcttttt catttctttt
     7201 atattgttat aacagtgaat aacataaaaa ttcccgacaa tcattgtaat atttttttgt
     7261 cacctgtatt agtatttaat ttataggaaa ctataaaaac tccctagtga atttacacct
     7321 ttgggagttc ctgtttggac ggtatgcata atacatttaa atcaatttaa cattaacacc
     7381 tcagcatatg gtatctccac atatatacgg tctttctttg gatagtactc aatatttatg
     7441 ttgatagcac ttccattgtc taatatagct ttagcccggt atgtgttatc tccattttca
     7501 tgagtaatcg tcacatcatc acatgatcga tttaagttgt actgttcttg taaaatctgt
     7561 gttaccaatg gtttcgatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaatg
     7621 atacctataa tagccaagac aacgataact ataatgataa acttcttgtt catatttact
     7681 ttattcaact ttttatctcc tatctccaaa ataatttgct cttatccctc ttatcagtac
     7741 attgacatcc ctaaaagtac accaaagatt gctccagcta cccctgctat catcaaaatc
     7801 gtacccattt gttcatcttt atctcgatat aagtagccca taataacagc aacagcacca
     7861 aatacaacag gaataaatgc taacgctact accatagaaa tccaaccaat aatcaaatac
     7921 agcttccctg tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca
     7981 gtacttttct ttactgcgtc tgctccagtt gtctcatctg tttcttgtag tgctccgcaa
     8041 aactggcaat acttactttc atctgttatt tctttgccac attttctaca atacatgtct
     8101 tctcctccta aaaacattca attgtttatt agttaactgt ttactttatt aaatacatca
     8161 gaaaacagct cattcattgg tacacttaat atctcagaaa gcatcttcat ttgatagggt
     8221 ttaggaatcg ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt
     8281 ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga ttacttactt
     8341 ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat
     8401 aaaatataaa aaaacttcaa ataccattac aaatacaaca acttgttgta ctatactgag
     8461 atgggggtgt attaatgaaa cagaaacaat atgcaaaggt cattagagaa aagcgaaaag
     8521 ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt ctctacacaa caggcagtag
     8581 caagatggga aaattctgta acagagccta acctcgatag cttaaccgct ctttctagag
     8641 ccctaggaac ccctgtaagc cattttctag acaatgttgt cgtagattat gaggaagagt
     8701 ttttggctct ctatcgttct cttagcactg aagatgcagt tcgaacaatt gactatatga
     8761 agctattaaa aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata
     8821 acccaccgaa ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa
     8881 cctgtctagt ttaatacaat tgggtctttt ctcttcctta ctctccgtcc atcatctctt
     8941 gttacacttg taaattgata aagaggaact aattcaactc ctgataatat caattctttt
     9001 attacttcat ctatccctgc tccttttgct aggtagacta aaaaactact acttgctttc
     9061 acattgtata gtctcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca
     9121 aagtagcctc tgttttcttt taaagcattt tccacgctgt tattcccaac attacgtaga
     9181 cgttcacaaa cagtgttagt aagttctttc tctgtcatta gatatgttcc ttgccacgat
     9241 aagccctgta aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctaagttct
     9301 gatcgggaat tttgaggaaa tactctttta gcctcttttt cgttaaaaca tatcttaacc
     9361 cattcataag aaaaatattg atatgtaaaa tgaaattttt ccagaagtgg aaggatatct
     9421 tcattcaatt tttgaatcac agaagttgca ttaaaaacgt ctaatcgata gacattgctt
     9481 ctcttatgat taaagtttcc taacgctttc tcttccttta acctatttag gtattcgagc
     9541 atcttcaaag gaacatcttc atcatctaat ttttttatca atcctataaa agtcataagt
     9601 gatagtaact tattacattt atctatgtca taagttttac catagtaaaa actcattttt
     9661 ttagctaatt gttcccccga caaaatacaa gtcacacttt ccttactatc atcaaaaaaa
     9721 ttaacatcag aaagcaagac atcatagaca cgtcgcattt cctctagata attccttatt
     9781 gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa aaagtacttt tgattcctct
     9841 ttcaattttc gaaaatgtgt aggatacaag tctaggttct ctaatagcca attaaatgtt
     9901 tctacccgac tgtctttctt taaaattagc atcacaactc caataaaatt ttcactgata
     9961 tcacaattac tggaatgaca ataaaaaatg gaatgcccag ttttactggt aaaaatacta
    10021 gcagagggat tatgatcgtt gtgaaacaga catcttaagt ttttacttga cttaagtaat
    10081 ttattcatat caatggttaa caatctttca taaatctcat taaaagacat gtcggaggaa
    10141 tcaaacaggc agtctttaaa ccactgtttc atttcttctt tgtcgtcatt ttttatcaac
    10201 ttaacaaaat cggtttcatt gactagttca tactctctcc ctgaatcact ttgacaattt
    10261 ttagaacatg tcatggtaaa atcaatcgga agctcagtaa tatctaacat gttgtcttca
    10321 ttaattatta tcactgcatc tgacttatat cctccaaaaa acattctatt cgaagcattt
    10381 acattgtcat cacacccggg gatgttcttt ttcaagtatt cgtaaacagc aaccatttta
    10441 ccatagtcag taattgcttc ttttaataga aagacaatct tgtatttttc tagattttca
    10501 gagtaccgaa tacttttttg tatcgcaaac gcagaattat gaactaaatt gatagataaa
    10561 accttttcta tgcttaagta tctactatca tctttactca aatctactac cttccccttc
    10621 tcatctttaa cagtgttatc gacatccaga ataattactc tggtttccac aaaactctcc
    10681 ttgtcattgc ctttattctt tgtcttgctt ggaataaatg attgtgatga atttagtatc
    10741 tctacaaact catctcttgt aacttctcta tagttatcta aacacctatc tcttacatca
    10801 gttttttctt tttttgtaac tggcttctct aataattttt ctttatctac ataaattgga
    10861 atattaatca ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa
    10921 aacatacaga taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac
    10981 tcaaccttta taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt
    11041 atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga
    11101 tacacctaga tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat
    11161 aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg
    11221 tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc
    11281 ttgaacaaat tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt
    11341 attaaccgat taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc
    11401 gataaaaaag aggtgtcaat atctttgtaa aatttattta caacacccct ttttcgaggt
    11461 atactaattt taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa
    11521 cgaaaaaaga tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa
    11581 ccagcatttg gtcttatgat taacaacggt caattaattg acaaaaaaac tatctacgaa
    11641 tgggagaagg gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga
    11701 aatatgagta tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaattatt
    11761 ctttatagag atagcattgt actagatggt ataacatttc cagataaaaa cctgttccag
    11821 catcttcgtc aacagttccc accagtacac tctaatttag atacatggct ggatagatat
    11881 tccaaattag aaccagagat gcaagaattt attgctaaca agacttgtaa taaagttaaa
    11941 aatgaaaaaa ttagtctttt caatatctta aaaatagaag aattattcat caatgctatt
    12001 gtagaagaat ttgacaataa cattcttttc ctcactagca gtatagaaga actactagaa
    12061 agaatggtag atgaatggct acctattcaa ttgaaagaca tgtcttatcc cgaagaagct
    12121 gtgagagaaa ttactgataa tataaacaag ctagaacaaa cgatttcatc aattgggaaa
    12181 aaatacacta agaaaaaaat gaagggaggt gataccatct agtggtaagt actccttgta
    12241 aactaagagg agatgatttc atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag
    12301 tggcgtggat gtataaagga cacattgcca ccgatccacg aactggtgaa aaaataaata
    12361 ctacgagaag aggttttagt agcaaacaag aagctcaaaa agattatgac gagtacagac
    12421 atcaaatatt atacggtgtt aaaaagaaag cacctgatat gacatttgaa gacctgtata
    12481 atgaatggat aactcatcag cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa
    12541 gatacgcaaa taatcaaata cttccagcat ttggtaagct taaactatcg aatatctctg
    12601 tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt tacgattata
    12661 tgagaaaaca gactgcacaa attttacgct acggggttgc aatgcaatat attgacaata
    12721 atcctatgga aaaaacactt cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt
    12781 actcaaaaga ggagcttaat aatttactgg atgcttttaa agattttggt aacatgaaac
    12841 aatacgcctt cttcagatta ttggcataca caggtatgag aaagagtgaa gtactcgctt
    12901 tacagtggaa agacatagac acgtttaata aagaactaca tgtcaataaa actcttgctg
    12961 tagatgaatt tggtaaagtt ataattcaat ctccgaaaac aagagcttct agacgagtta
    13021 tctctttaga tactgagact ctttctatcc tcaacaactg gaaattacag caaaaagaag
    13081 agtatttaaa acttggatac aatactagct ctaaagagca acatgttttt acaacagtta
    13141 aaaacactct atacattcca aacactgtta atgattggtt aagatatatt ttgaaaaaat
    13201 ataacttgcc tcgtattaca cctcatgggt ttagacatac acatgctagt ttattattag
    13261 aagcaggaga atctgtcaag gtagttcaac aacgattagg gcatgaaaat agtaaagtga
    13321 cattagacat ctatgcacat attacaaata atgcacctaa gaaaacaggt caagactttg
    13381 ctgatatgat ggctcatcag taaaagagct ggtcaaaatc tggtcatttc tagtatacag
    13441 gcaaaacaaa aaagctagaa cccttatata tcaagggttc tagctcctat aacaggttta
    13501 cccaacactg ccttccatct cataactgat caatcgattg agttcgactg catattccat
    13561 tggaagttct ttggtgaatg gttcaacaaa gcccatgacg atcatttcag ttgcttcaga
    13621 ttcggtcagg cctcggctca tcagatagta gagttgttct tctgagattt ttgagacttt
    13681 ggcttcgtgt tctaaagaga cttggctgtt gtggatctca ttgaatggga tcgtatcgga
    13741 tttcgaccat tcgtccatga tgatcgtgtc acattcgata tgagagatcg aacccgcact
    13801 gtttttggca aaggttactt gtcctcgata gtttacttct ccgccgtctt ttgaaattga
    13861 tttagagacg atagagctag atgtatttgg tgcattgtgg atcatttttg cacccgtatc
    13921 ttggatttgg tttgctcctg caaaggcgat ggagagcatg gtgcctcgtg caccttttcc
    13981 atctaagtaa acacttgggt atttcatcgt tgtttttgca cctaggtttc cgtcgatcca
    14041 ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt actaagttgt acacattgtc
    14101 tgaccagttt tggatcgttg tgtaacggca atacgcatct tttcttgtaa agatttctac
    14161 gatggcggca tgcaggctgt tacttgaata agtaggtgcc gtacaacctt cgacatagtg
    14221 gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg aattgccccg tgttttccgc
    14281 attgatccgg aagtacgttt gtaatggaac atcgactttg acgccttttg gcacgtaaat
    14341 aaaggtccca cctgaccata cggctgaatt cagcgctgct aatttgttgt cggttggcgg
    14401 tacaagtttt gaaaagtact ctttgaataa ttcagggtat tctttcaaag cagagtcagt
    14461 gtcagtaaag acgattccta acttttcgaa ttcatctttc atattatggt acacgacttc
    14521 tgattcatat tgtgcggaag caccggctag ataagctcgt tcagcttctg gtataccaat
    14581 cttttcaaat gtatctttga ttttttctgg tacgtcttcc cagtcacgag cgggtttgtc
    14641 acttggtttt tggtaatatt tgatggcatc gaaatcgatg tctgaaaggt cggggcccca
    14701 tgtttgcatt ggcattttgt taaatgtttc tagagatttc agacggaatt caagcatcca
    14761 ttctggttcg cctttgattc ttgacatctc acggatcact tcttctgtca agccttctcc
    14821 tgtactgaaa accggttcga catcatcgtg aaacccgaat ttatattctt ctaattctgg
    14881 tacgcccatg ttctcactcc tcttccttgt ggtaatgatg gaaagctgcg gtaccatcgt
    14941 tttccattgc ttttcctagt gctttccagg caagtgtcgc acatttgatc cgagcgggaa
    15001 atttagctac accaccaagc atagcagcat cgcctaattg gtcgggatct ttgacttctt
    15061 ttccttggac aagctgtaga aagtctacag ttaaatgttc tgcttcttct attgattttc
    15121 cgatgacggc gtctgtcatc atcgaggcac ttgccgtact gatggagcag ccactgccgt
    15181 caaaggcgat atttttgatg attccgtctt ttacatcgac ttgcaattcg atcacatctc
    15241 cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc agtcaagctt ccacgatgat
    15301 gcggatggga agaatgatcc aagatcactt gacggtataa attgtctaat ttagatagtc
    15361 ccatgttgga aaaactcctt tgttgccatg attgcttcaa ctaaacggtc ggcgtcttgc
    15421 gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg atacttcaag gtacttcaat
    15481 agcggttgcg cacaatgatg acctgctctg acggctaccc cttccatgtc taaagctgtc
    15541 gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa cgactcctgt ccgaaaattc
    15601 gggtcttgtg gaccataaac agtcaatcca tcaattgctt gtaattttgg caacacataa
    15661 gcggctagtt cttcttcata tcgatggata ttgtccatac cgagtttttg caaataatcg
    15721 attgcatggc ctaatgcgat agcgccggcg atatttggtg tccctgcttc gaatttccaa
    15781 ggtagttctt tccatgtact gtcgtataat tcgacaaaat cgatcatttc gccaccaaat
    15841 tcaacaggtt ccatttcttc taaccatttt cgtttaccat ataaaacacc aatacctgtt
    15901 ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac agtctagtgc ttggacatca
    15961 atggccatgt gcggggcaga ttgagcacca tctactacga tgaccccgcc gttttcatgg
    16021 acaagttcag ctaattcttg aacaggattg aagacaccta agacattgga tacatgagca
    16081 atggagacga ttttggtttt gtccgtaatc tttttcgctg catcctccat atccaggaag
    16141 ccttcttctg ttagttcgat atatttcaag acagcacctg ttcgttttgc caactgctgc
    16201 cacgggataa cattagaatg atgttccatg taagagagca ctatctcgtc tcctgctttg
    16261 atatagcggt cgccgaaggt tctggctacc caattcaagc ttgtcgtcgt accacgagta
    16321 aacagcactt ctgctgtttc ttttgcatgg atgaacgccc gtactttttc tcttgcttgt
    16381 tcataatcat gggtcgcgcg ttctgctaac gtatggacac cgcgatgaac attggcgtta
    16441 tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt ttggcttttg ggtcgtcgct
    16501 gcattatcca gataaactaa aggttcatca ttgacgtctt gaaataagat aggaaaatca
    16561 ttgcgtagct ttttagcatc gatcatccat tcaacttccc ttcgattact tcgactaatt
    16621 ctttccgaac ttgttctaca gggattgctg ttaagacaga accgaggaat cctcgaatca
    16681 ctaagcgctc tgcttcttct ttatgcaatc ctctactcat cagatagtac atttcttctg
    16741 gatcaactcg tcctacactg gcagcatgtc cagcagttac ttcgttttca tcgatcaaaa
    16801 gaataggatt cgcatcacca cgtgctttat cagaaagcat caagacacga ctttcttgtt
    16861 gggcatctgc accttttgcc cctttcaaga tgtggccgat accattaaag gtcaatgttc
    16921 ctttttctcg gatcactccg tgttgcaaaa tatgcccgat cgtatgaggg gctttattgg
    16981 ttactcgtgt atcgattcct tggatttgtc tgcccgagct gattgccacg attttgactt
    17041 ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc agcgatcaca tgaccgtcat
    17101 tcatgacacc aattgcccag tcgacagaag catctcgtaa aatgtgccca cggcgattca
    17161 tataagaagt aatattttct cctaattgat ctacggctga atatttgatt tttgaaccag
    17221 ctttagcgat gacttccacg atgatattac ctgatgattt tgctacttgt tctttggttg
    17281 tctggaaacg ttccagataa gaaaattcac tatgttcatc tgccacgatc aacacatgct
    17341 tgaagaagtg ctcatcggat gcgccatctt gaatgaataa ggactcgagt ggttcatcga
    17401 tcacgacatt tttaggcaca tacaggaaaa taccgctgtt cataaaagct acatggagtg
    17461 cagtcaattg gtcttcgttc atgtttactg cttttttcat ataatattct tggactagtt
    17521 ctggatattc gatcattgca gtgaacaagt cggtaaaaat gactcctttt tctgctaatt
    17581 ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg gacaagcaca ggattgtctt
    17641 tcattgcatc aaatgccgga atcgtgcctg tttcactgat ggtttgtgtt tctttcacat
    17701 caaataacga ccagcgatgg aatttcaccc gatcgatatg cggcaatggc agttcatctg
    17761 ctttcgctag tgcgtcttga cggaaggttc tcatccaatc aggttcgcct ttacttaaag
    17821 aaaaatcatt taccgcgtca agataatcga gccagttttt ttctttcatt cttgctcccc
    17881 cttacgcttc ttcttcttta tagtcgattc ctaattcttt actgatacca gcgtagcctt
    17941 ctgcttccag acgtttagct aggtcagcat tccctgtcat gactactcgg ccttccatca
    18001 tgatgtgaac gacatcaggt gtgatatagt tcaataatcg ttgatagtgg gtgatgatca
    18061 atgcaccgaa attgtctccg cgcatttcat ttactccttt agcaacgact ttcaaagcat
    18121 cgatatccag acctgaatcg atttcgtcta aaattgcaaa tgtaggttct aacatcaaca
    18181 attgcaggat ctcattccgt ttcttttctc cgccagaaaa accttcgttc agataacgtt
    18241 cagccatttc ttctggcata tttagtaaag ccattttttc atctaacttt ttcaaaaatg
    18301 ccataacaga aattttgtcg ttttcatcac gtttcgcatt gatcgctgca cgcatgaatt
    18361 ccgcattcgt gatacctggt atctcacttg gatattgcat agctaaaaat aagccaagtc
    18421 gcgcacgttc atctacttcc aagtctaaaa catttttccc gtcaaacaaa atttcgcctt
    18481 ctgttacttc atagtttgga ttgcccatga tagcggcaga taaggtagat tttcctgtgc
    18541 cgttaggtcc catgatggca tggatttctc cggttttcat tgttaggttt acgcctttaa
    18601 ggatttcttt ttcctcgatc gacacatgta gattttttat ttctaagaca gacatgcatt
    18661 atacccccaa attctctttc gttctcactc attttagact aattgagaat gaagcgcaat
    18721 gaccttcctt actaaaatca aatatctttt atttattatt aaacaatttt gatttctaaa
    18781 ttagaatagg tataaaaaag atgagaaata agccattaca gacttatttc tcatcttttt
    18841 tgttccatac cttattttgc atcagccttt actggtttaa cagagccgcc ccatttgtct
    18901 gtgatccatt cttggacttc tttgcggtga agcacttcaa tcagtttttt gattttgtca
    18961 ctgtcttcat cacctttacg agtcgcaata atattcacgt aaggagagtt gtccttttca
    19021 agcaatacgg catcttctaa tggattcaac cctgcgccgt aagcgaagtt tgcgttgatt
    19081 gcaactaggt caccttcatc attttggtac gtagaagcta aaagtgatgg gtcgatcgta
    19141 tggttgaact tcaagttctt agggttttct tcaatatcat cgaatgttgc tgtttccaga
    19201 tctactccat ctttgacttt gactaagcca gcatcttgta gaattgtgat gatgcgtccc
    19261 cagtctgatt cagaatttga agtaatgatt gttgcaccat cttttaattc ggaaacatct
    19321 ttgacttttt tagagtacaa gcccattggt tcgatgtgga cagcaccggc attgacaaag
    19381 tcatatcctt tttctttgat ttctttgttc aaataaggaa tatgctggaa gtaattggca
    19441 tcaatgtcgc cttcttccaa agattggttc ggtaatacat agtcatcgaa tttgacgatt
    19501 tcaagatcga ttccttcatc tttcaataat ggtttcacat gttccaaaat ctctgcgtgc
    19561 ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg ctttatttgc tgaatctttg
    19621 ctgtcgctgt tgttaccgca tgctgctaat cctacagtca gcaaaagcgc tgctgcaaat
    19681 ccaaaaattt tctttttcat atcctacatc cccctatatc ctattatctt ttgtctgttt
    19741 tcttagttaa gaaatcaccg attccttgaa cgataaatac gattagtaaa ataattaaag
    19801 tcgcaacgaa tgtcactgtt agattaccac gctggtagcc ttcctgccaa gcaagacctc
    19861 caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa tccgatcatc gagatcgttg
    19921 taactgttaa tccagaaatc aatgcgggaa tactttcagg tatcaagacc ttccagatga
    19981 tttcccagta acttgctccc attgcatctg ctgcttccag tacaccaggg ctgacttcac
    20041 gaaaagcaat ctctactaga cgggcataaa atggtgctgc ggataaaaca agagctggaa
    20101 tggctgcttg cgcacctagc atagtgccta cgattgcttt agtaaatgga atgatcaaaa
    20161 ccatcaggat cataaatggc gttgaccgga aaatattact gatgatcgat acgattccat
    20221 aaatgattcg agcatacggt ttgtttttgc gtccaataga gtaaaggatc aatcccaaaa
    20281 ctaatccgat gatcgtcact aagaccattg agattaatgt catatatagt gtatcaagga
    20341 tagcctgatt catcgtttcc gggttgattt gtttaaaatc tagatatgtt tctgaaaagc
    20401 ttttatccac gatggatcac ctcaattcct actccttgtt ctttgatgaa cacttctgct
    20461 tgatctaatt ctttggtttc accagtcatc aagacagtca atgaaccaaa ggaaccttct
    20521 ttcacttgtt tgatctttcc atacacaacg ttgacatcga tgttatagcg tcgaatcgct
    20581 tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg tcaaagttgc caaacggccg
    20641 cttggatttt ctttgatgag ttcagctaat agttctgtcg aatcagcttc tggttcgatg
    20701 tcttgttgta caaagcgttt ggttacttct tttttcggtt gtctgaagac agtcaatacg
    20761 tctccttctt ctacgacttt ccccaactcc atcacagcga ctttgttgca gattttgcgg
    20821 atcacattca tttcatgtgt gatcaagacg atggtcaaat tcaatttctt attgatcgct
    20881 aacaataagt ctaatacttc ttctgttgtt tgcgggtcca atgcgctagt agcctcatca
    20941 caaagcagga tctcaggatc atttgccaat gctcgtgcga taccgacgcg ttgtttctgt
    21001 ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt ctaacccgac taaacgcaac
    21061 agttcttctg cccgttcttt acgtttgctc tttggtacgc cagctaattc cagcggcagc
    21121 tggatatttt caagtacagt tcttgaccat aacaggttga aatgctggaa gatcataccg
    21181 attttctttc ggaatgtacg taattctttg ttttttaatt ttgtaatatc ttgatcgttt
    21241 acgatcactt cgccgctcgt gggaagctca agtccattca ggagacggac taatgtactt
    21301 tttcctgcgc cagaatagcc gacgattcca taaatatccc ctttttcaac gtttagtgaa
    21361 acgtcattga cggcttggat gtttccgttt ttcccattga atgtcttttt gacattcgtt
    21421 agttggatta atgacatgcc attcacatct ttctcttata atttttcaaa caaaaaagcc
    21481 ttcgccctct tgccttaagc aaaaggacga aagcttgaac ttccgtgtta ccaccttgat
    21541 ttaccgttac ttcgcaataa cagcctcttc cagtacttgg acttagcttc tttccgtata
    21601 ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt acgctcgctt actctgctct
    21661 aagaccatct tccatctcat tttcgttacc cattttcagc aaacagggct ctcttcaaac
    21721 gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt catataggat tgcttatgat
    21781 tatagcaact ggaagaatca gtgtcaactt ttttcaagtg attttctgat tttataattc
    21841 gtcaaattct tttggatcaa catctttgaa acttaagatc cacgcattca tttcatctga
    21901 atcattcaag atattgatat cttcaccgat tttatcatta acagaagaaa ctgtacccgt
    21961 tagcgggctg gcaaattcat tgaccgcttt ttccgcttca acttcgatca gaggttcatc
    22021 tttttttagc gattgtcctg cttttggcag attggcaaac gtgatgtctc caagttcttc
    22081 ttgcgcttca tttgtcaaac caacacagta ttcttttcca ttaaataaga tccaaaggtt
    22141 gtcctttttc ttcaaatgct tgcctgccat tttatatcag ttctccttca taaatttttt
    22201 cattgaatcc aattgcaaca agctggtcct ctcgtaagat cagtggtcgc ttgatcagca
    22261 taccatctgt cgataagact tggctcgctt ttgaagccgt aaactcgcct acttgatctt
    22321 tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa caggcgacgg attggtactt
    22381 gactcgtttc tagccaagtc tcaatcgttg aggccttcgg tacttctttg atcatatcaa
    22441 cggtttgata actaacgcca tgctggtcta accacgcttt ggcttttttg catgttgaac
    22501 atttcgggta ccagtaaaag gtgtacaatt ttcttacctc cttgaagatc ttgcaggtgc
    22561 ttcattttgg tatctcatcg ataatatcaa cccgataccg atcatgttcc ctaaaatcga
    22621 ggatcctccc tgactaatga aaggcaatgg gatccctgtt aaaggaagta atccgatatt
    22681 tgctccaata ttctcaaaga catggaacag cagcatcatg atcaaaccgc ttgcgatata
    22741 ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc atacgataga ttaaaataaa
    22801 atacaagaaa atgacgaatg caccaccgat aaagccgaag ttttcaccga taacggtaaa
    22861 aatcatatcg gactcacgaa caggcacata gatattgctt acgttgaatc ctttaccaaa
    22921 caagccaccg gtaccaattg ccaaaagtcc ttgggccggc tgataactca ttcctgacgt
    22981 atcatggaaa gggtccagcc atgaatcaat acgtgcaaac tgataagatt tgaatccaac
    23041 tttataaagc aagtctcttc cgccttcagt cgtaaccaag aagatcgttc ctgctccaac
    23101 gactactgcc aagattacta caggtgcgat gatgcgccaa gagattccag acatcaggaa
    23161 aatcccgcca aagatcgcaa gaaagacaag catcgttcca aagtcatctt gggccatgat
    23221 caatgctaaa accggtattg tcacgatcaa gagcttccca atcagccaaa agtccgattt
    23281 catcgtccgt tcttttactt gcgtattatg tttagttacg atcagtgcca tcatcaagat
    23341 gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac gcaccgaatc gaaaccagtt
    23401 tttagaccct gttgaagcag ctaaattctg atcatagaat ttgagcaaga gtcccattac
    23461 cactaacccc aaaccataaa ggtaaggagt cagtttccag atccatttgg aattcaaatg
    23521 catgatgaca acgattgctg ccgctccaac taaataccag acaacttgca tccctacgcc
    23581 tttcaagact gctggattgt tcggatcatg gctaagtgct acatataatg agagcagacc
    23641 aatcaaacat aacagaaaga ctggtaaaat cacgccataa tcaatacgat tatcgttcga
    23701 agcttttaat tttttttcca tgtttcatcc cttttcttaa aaaccatact gctgattatt
    23761 atagcggtcc agttagataa tggaaagtct ttccatagac atttgggaaa aattaacttt
    23821 ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc tactgcttat ctttagtgta
    23881 tcatagaact ttttttgata catgcaatta tctaccagct gagattctcc tcgctgtgag
    23941 aagtttctgt gctttcattg tatcgaagga tctcttcggc aatctctagc agctgatcag
    24001 aaaccagatt ggttcgttgg atgaaataaa aatatcgatg gaaattcaac ggagtcattg
    24061 gcacattttc tggaacagag cgcttagaca aaatcgagcg accgattcct tccttcagca
    24121 acgcaacaat tacatcgtta cttttcacga tcatcttctt atcttggata ttcatctctt
    24181 caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc ttctcttaca agccacaatt
    24241 tgctttttgg gtccccagcg atgactagct ggtcttccat caaagagtat ctttgtacgc
    24301 cggaagtctc taatggcttt tcgataaaac caaaatcgga ttcatgtttc gctacttgtt
    24361 ctaatgattc atgagaattc gtgatttcga tctcaaaatg cactttagga aaccttttga
    24421 tcagatgttt catcaactga ggcaaaagat agacagcaaa ggtgtgggaa gcaacgatcc
    24481 gacatacttc ttcttcattt tgttcattga gcaatttttg ctgagtttct tcccattctt
    24541 ctaacaaaac cagtgtccgt tcatataata agtcggcttg cttagttgga agcatctctt
    24601 ttcttccgtt acgttgaaaa agatcgatat tcaattcccg ctccaactgt ttgatttgat
    24661 tcgagactgc tggttgagat aaaaacagta cttctgctgc tctggaaaaa tttcttgttt
    24721 catatactgc tcgaaaagtc gttaataact taaacatggc accacttcca taacttttta
    24781 tgataattac tataaaaact atttgattta aatatacaaa aaaccatcta taatagaaat
    24841 gtacaacaaa acagttgctg atccaactag taacttagat gaagacaatc agctgtctgc
    24901 ttgctcgtaa tcatacattc ctattcattt tacaatgtat tcgttcatct tggtactaaa
    24961 atctattaaa taaaaaggag aatattatat atgaagaact ttttgaaaat tattccgatc
    25021 ccgatctgcg ggttgatcct tgggttagct tcccttggaa atctgtttaa agactatcac
    25081 catgttgctt taggcaatct aactggcggt atctctatga ttcttatgat attcattttt
    25141 gttaaattgt ttatcttatt cgaacacacg aaacaaacgt tgcatgatcc aatcatcgcc
    25201 tccgtttcac caacttttac gatgtcactc atggttattt gtacttattt tgtttcatat
    25261 gaaagtattg caccaattgt caaattcatt tggctgtgcg ctgttatctt ccaagtgatc
    25321 cttgtctttt atttcaatta tcaccatgtt atcaaagctg atctttcaat tgaagcaatt
    25381 tatccaagct ggtttatcat ttacgttgga tttggggtca tcactgtaac tgccgggaac
    25441 ttcttcccac ttattggaaa aattttcttt tggatttcat tgatctgcta tgcgttctta
    25501 ttgccgatca tcatcaaacg tgtctttttc gtaagaaata tggctcatcc gacacgtccg
    25561 ctgattacga tcgttgcagc acctggttct ctttgcctaa caggttatct taaaaacttt
    25621 gctgaaccta atttttatct tgtcgtagca ttgttcgctt tatctcaagt attgtatttc
    25681 gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt tttatccaag ctatgcagcc
    25741 tttacattcc cgcttgttat ctctgcaaca gctatattta ctactgttca ttacttcaac
    25801 agtttaggta tctatagtag cgtattggat ttcttaaaag ttgcggaatg tctgtttgca
    25861 agtgtagttg tcttctacgt tcttgctcat tacgtacgtt tcttaattaa agaacatagc
    25921 aaacatggaa aatttgtttc taaaaaaatc gcttaataat cgattcaata ggttgtgaaa
    25981 aagctgttct gcatcaagta ataagaacgg ccaaacaaaa acagctgttc atgtttttgt
    26041 actggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggtcgta
    26101 a
//
LOCUS       DAZKAY010000036        25928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000036 DAZKAY010000000
VERSION     DAZKAY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.036"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..372
                     /locus_tag="ACG8WP_001368"
     CDS             112..372
                     /locus_tag="ACG8WP_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFP9027679.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            413..640
                     /locus_tag="ACG8WP_001369"
     CDS             413..640
                     /locus_tag="ACG8WP_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027680.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            705..1277
                     /locus_tag="ACG8WP_001370"
     CDS             705..1277
                     /locus_tag="ACG8WP_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027681.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1278..1589
                     /locus_tag="ACG8WP_001371"
     CDS             1278..1589
                     /locus_tag="ACG8WP_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9027682.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1580..1840
                     /locus_tag="ACG8WP_001372"
     CDS             1580..1840
                     /locus_tag="ACG8WP_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027683.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2001..2360
                     /locus_tag="ACG8WP_001373"
     CDS             2001..2360
                     /locus_tag="ACG8WP_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027684.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2357..4000
                     /locus_tag="ACG8WP_001374"
     CDS             2357..4000
                     /locus_tag="ACG8WP_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9027685.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4035..5117
                     /locus_tag="ACG8WP_001375"
     CDS             4035..5117
                     /locus_tag="ACG8WP_001375"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFP9027686.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5161..6333
                     /locus_tag="ACG8WP_001376"
     CDS             5161..6333
                     /locus_tag="ACG8WP_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9027687.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6320..7024
                     /locus_tag="ACG8WP_001377"
     CDS             6320..7024
                     /locus_tag="ACG8WP_001377"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9027688.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7029..8189
                     /locus_tag="ACG8WP_001378"
     CDS             7029..8189
                     /locus_tag="ACG8WP_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9027689.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8265..8543
                     /locus_tag="ACG8WP_001379"
     CDS             8265..8543
                     /locus_tag="ACG8WP_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFP9027690.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8524..8895
                     /locus_tag="ACG8WP_001380"
     CDS             8524..8895
                     /locus_tag="ACG8WP_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFP9027691.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8911..9315
                     /locus_tag="ACG8WP_001381"
     CDS             8911..9315
                     /locus_tag="ACG8WP_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027692.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9312..9722
                     /locus_tag="ACG8WP_001382"
     CDS             9312..9722
                     /locus_tag="ACG8WP_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027693.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9783..10370
                     /locus_tag="ACG8WP_001383"
     CDS             9783..10370
                     /locus_tag="ACG8WP_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9027694.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10443..10910
                     /locus_tag="ACG8WP_001384"
     CDS             10443..10910
                     /locus_tag="ACG8WP_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP9027695.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10956..11264
                     /locus_tag="ACG8WP_001385"
     CDS             10956..11264
                     /locus_tag="ACG8WP_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027696.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11459..18193
                     /locus_tag="ACG8WP_001386"
     CDS             11459..18193
                     /locus_tag="ACG8WP_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9027697.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18197..19069
                     /locus_tag="ACG8WP_001387"
     CDS             18197..19069
                     /locus_tag="ACG8WP_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFP9027698.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19066..20115
                     /locus_tag="ACG8WP_001388"
     CDS             19066..20115
                     /locus_tag="ACG8WP_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027699.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20119..20943
                     /locus_tag="ACG8WP_001389"
     CDS             20119..20943
                     /locus_tag="ACG8WP_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027700.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20961..23003
                     /locus_tag="ACG8WP_001390"
     CDS             20961..23003
                     /locus_tag="ACG8WP_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9027701.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            23017..23166
                     /locus_tag="ACG8WP_001391"
     CDS             23017..23166
                     /locus_tag="ACG8WP_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027702.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            23163..23609
                     /locus_tag="ACG8WP_001392"
     CDS             23163..23609
                     /locus_tag="ACG8WP_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027703.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            23611..23748
                     /locus_tag="ACG8WP_001393"
     CDS             23611..23748
                     /locus_tag="ACG8WP_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9027704.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            23785..24078
                     /locus_tag="ACG8WP_001394"
     CDS             23785..24078
                     /locus_tag="ACG8WP_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027705.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            24075..24299
                     /locus_tag="ACG8WP_001395"
     CDS             24075..24299
                     /locus_tag="ACG8WP_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9027706.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            24296..25315
                     /locus_tag="ACG8WP_001396"
     CDS             24296..25315
                     /locus_tag="ACG8WP_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9027707.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            25504..25577
                     /locus_tag="ACG8WP_001397"
     tRNA            25504..25577
                     /locus_tag="ACG8WP_001397"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25538..25540,aa:Ile,seq:cat)
BASE COUNT         9257 a         3981 c         5461 g         7229 t
ORIGIN      
        1 caacaacaac aggatttagt tttatttata ctgggaaggc tacgggagtt actcgttcag
       61 ctacgtttaa caatacatca acagctggtt atgcgcttgc ggaggtacaa aatgaatcat
      121 gagaagttta tcgaaaaatg caaggctatt gtacgtgaaa gaattgaaaa tgagattgct
      181 gacctaagtg gagcagtgcc tgagtttagt attttcatag tctggtcatg caaaacactg
      241 caaaatagca aagcattagt cagcgctagc ttaaaaggag caccgtattt tgaaattacg
      301 ttgaacgggg acaaaggtga aatctatgta gatacttatc tcaaaaaatc aaatgaatgt
      361 atcaaagtct agcaggtgct aggctttttc tttacataaa ggaggctgca taatgagaaa
      421 ctactggtat gtatcactaa ctaatgaata tcctcgaacc attggtgatt gttcagtgcg
      481 tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt gtcgaaatgc taagagaagc
      541 tacaccaaac gaagtggata aatgcaagct gatatattgc ggtcatggct attggaaaga
      601 cgagtatatc caatacaaca ttgagaggta catcaaatga atgataacta tgattacatc
      661 aagttgttag caagtaatta ttagatgttt ttaggaggaa aactatgaaa ttaaaagatt
      721 atatcagaga agggtataac gttgtaactg cacccaatct agcttacaaa attcaagaag
      781 attatcccaa tgctttagtg tttactgata gagctttaga tgccattcct atagggaagt
      841 tattagtaga tcgtttttat agcaataatc cagctgttct tagtgctaag ccacttcaaa
      901 acgcttatgc tattgaacgc tttacttgcg agtttactgg atatgagacg gtagtaccaa
      961 tgacaggtga gacaggaaga aaagtaattc aacagatcaa aaagaggatt gctgaattga
     1021 cgttagatga tggaagtaat acgaaccttc tagaaatcaa actaagagat actgactcag
     1081 taccagaagt ttattataaa ggtcaacgaa tggatgagat gccgataggt cttgtcgata
     1141 ttacttatca ttgggatacg gaagaatatg aatgccaagt accaaaagga gctaacgaca
     1201 tcactattga atattatgat tcacataata acaagtactt agatagaaaa atcattgggc
     1261 acaagagaga tatgtaaatg attgatgttt caagtaaaca agcaagagct aggttctatg
     1321 gatcaactga gtggaggaaa cttagaagtc aatgcttaga gcgagatcat tatgagtgcc
     1381 aatggtgtaa agcagagggt agactaacaa ctcaacatga ttcaattctt gaagtggatc
     1441 atataaagga gctggagtat catccggatt tagctctaga tataaacaac ctaagaacct
     1501 tatgcaaaga ttgccataac aaaaggcaca accgtatgaa atatcgtggc caacaaaaga
     1561 aaagaaagtg ggatgatgaa tggtggtaag aaaaacagca aacagagatg atgtcaaacg
     1621 agcagaagca atcgctatgg ttgataaggc tattgataag ttggttctat ttaagagtca
     1681 gctcattgaa ggaacaacaa ctgtagagga tatatctatc aacaatagac taataggtaa
     1741 cgctaatgaa gtaacaggga catttaatat taatgtagac attgattata ccttgagtcc
     1801 atttgcacat attgtagaaa gcaacgagca agacggctga taaatcagta taaagcgaag
     1861 atatatagag tgaaataccc ccggtcaaaa gattttgaga agaaataaaa tttttgggaa
     1921 ccggtggatg gggtcaactc tgcaaataaa tgatgctatt ttcacacgac cccctcccca
     1981 cgtagaaagg aagtgagttg atggctgatc tgcaagagag aaataggctt gtttctgctg
     2041 aagaaaggcg tctaaataaa ttgtttaagg atattgataa ggataaaaag aaggtcgttt
     2101 ctggtttgat tacgcaagcg gcacgcttaa aaattcttct tgatgaaatg tggattgata
     2161 tttccgaaaa aggtgattat gagcttttct ctcagtcaga aaatcaaaaa ccttacgagc
     2221 gggaacgacc agttgctaaa cagtataatt cgagagacca agcatatcaa cgtgtaatta
     2281 agcaacttac tgactatttg ccagaagaaa agcgcgaacc tacaaaaaat gcagcgctag
     2341 atggtagtga tttgctgtga caacattaaa gccattctat tttgatgaat acgtagattt
     2401 atacgaaaaa ggggtaatcc cttttaataa agaaagaatt cagttaatta cttatttaaa
     2461 aaacgaagtg ctagttcggg atgatatcta ttttgatgaa gaaatgattc aaaagtttat
     2521 caagtacact gaaaaaaatt tttttccttt agctaaatat caaaaattta tcacaccttt
     2581 cattttctgc tatcaaaaag aggacgacga ggtgtttttt gatgaaattc taaatagtat
     2641 tgcgcgaggc ggcgggaaaa atgggttcat gtcagcaaga gactcatttt ttattagccc
     2701 tctctacggt gtcagaaatt atgatgtaac gattacagcg aattctgaga agcagggaaa
     2761 ggtatctttt aaagaagttt atgaaatggt acaaatgcgc aaactggaaa aacaatttta
     2821 tttgactaag atggctatag tcaacaatgt aactaattcg atttttagtt ataggacaaa
     2881 caatcctaag actatggata gtgcaagaga tggctgctta gaatttgatg aaatccatca
     2941 gtttgaatct tcagacctag ttgatattca gcgtagtggg ctagggaaaa taaaacatcc
     3001 tcgaactttt tataatggta caaatggtca tgttagagaa ggtttttatg acaaaatgtt
     3061 ggaacgagca caaaagattt ttagtggaga gaacaagaat gatcgcttgt ttccctttat
     3121 ttgtaaactg gataacattg atgaaatgga taatcctaaa atgtggtcaa aggctaatcc
     3181 aatgttcgaa gaagattcag catattcaaa acgtctgttt tctacagtga tgaaagaata
     3241 taaaaaacta gaagatgagc catcaggtag gcgtgaattt gttgttaaac gaatgaattt
     3301 tacagaaggt gatgcagaat cagatattac gactcatgaa aaattaatgg ctactaaaca
     3361 agaattgcca aatttgaatg gtcgttcttg tgtggctgga tttgattatg caagtattcg
     3421 tgattttgca acagtgggtt tgctttttaa aattgatgat aaatttatct ggcagcaaca
     3481 tagttttgct aggaaaaagt ttctagatac attcaaactg aaagcgccta ttaaggattg
     3541 ggagaaaaaa ggtttagtga caatcgttga tgagccatca attgatcctc gtcatttaat
     3601 tgattggtta aatgaaaaaa gaaaaatcca tcaaatagaa atcgtttgtg ccgacggatt
     3661 tcgtatggat ttgctgaaac ctcttctaga gaaagaatca tataattttg agtttatcag
     3721 aaatataaga ggtgtgcagt caaaagttgc tcctattatt gaagatggtt ttgcaaatga
     3781 acggtttatt tttggtgatg atcctatgat gcgttggtat acaaataatt cgtttatcaa
     3841 agaagacaaa ttagggaata aaacattttt gaaaaaagag ccagtaaggc gtaaaacaga
     3901 tgggtttcat gcttttttgg cagctttata taaacgagaa gaaataactg atatggatta
     3961 tgaggaagca tttgatatgt tggatgagat tgaattttaa ggtatactcc ttattgaaag
     4021 gggtagttat agaagtgaaa aagagatatt ttctatttgt tacaatattt ataataatag
     4081 caatttgtgt tttgacagta tttttaccac aaattactga gtatttaaat tatcggatat
     4141 ttaccacaaa agtagatgat agcgttacta gtttaagtag gctagaattt ataaaaatat
     4201 gtgcatctgt gtttggtcct attttgactg taatggtatt tgtgaatact ttaaatatgc
     4261 agaaaaaaac agagcaaaag caaaatgagc ttgaagtaaa gcaagctaag gctgagaacg
     4321 agagagcaaa aaaagagctg ttaacacaga tagatcgtga gttctatttt ttattaaata
     4381 tgtttattga aatacaaaaa gatcaaaatg ccaaacaagc tgttattgct ttacagaagt
     4441 tggcaatttt taacgaaaag ggttttaatc aagactatgc atttacttct aaagatttat
     4501 cagatagtga aattgggagt acaattgaac aatcaggacc gctgaagggc acagttgaat
     4561 atataaaatt tgaaacattt gatgtaagca agtcgatggg gaattatcaa acaaaaacaa
     4621 aagacgttgc tacaagttta cacgatcaga aagtagctat attgaatgaa caatatgaaa
     4681 agatgcaaat atatcttgga cggtacttca aaatgtttca ccgaatagtt aaaacattaa
     4741 atgagtatta tgacgattat aatgattttg atgtgaagag gtatacaaaa tatataggga
     4801 cattaagaac acaaatttct ccagctgaat tccaagtgat tttattcaat tctctatata
     4861 taaaacgagg ttttggctta ggaattcaac ttattggaag tggttttttt ggagatgatt
     4921 ttgattttga aactaatcaa cattttgaaa caagtatcaa tgaacagtgg tttctatcac
     4981 tatcaactgt tgacagtgat aactctataa agcgacaaaa attatctgaa tatattaaag
     5041 aattaggatc cgtagaatat aaaaaaatag ctaattttga aagtttatat aaactcttta
     5101 atgaaactaa gctctagaaa tagggctttt ttatttttct ttgaaaggtg gtgaaaacat
     5161 gtgagcttat ttgatatctt taagcagtca ataagaaatg aagagccttc tgagtggata
     5221 ccggattttg tatatggaga ggaagtatct gctcgggcat acctcaaaat tatggcaaaa
     5281 aatactgttt tagattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat
     5341 aaagatggga catcagactg ggattatatt ctaaatgttc gacctaattc tgatatgtca
     5401 gcagctacat tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagta
     5461 atatttacag atgataatca attgctgatt gctgatgatt ttagtcgaac ggagtatgcc
     5521 gtgtatgatg atgtttttac aaatgtgatg gtaaaaaatt ttgtctttga acgttcatgg
     5581 aacatgtctg acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga
     5641 ggtttatttg aagattacgc cgcgttgttc ggacggattc tagaagttgc aatgcgtaac
     5701 aaccaaattc gtgggtctgt atccattgat tcaacgggaa cggcgaatga tgaaaaagga
     5761 aaagatggta agactagatc tcaaagatta caagaatata ttgataggat ttggaactct
     5821 tttaaaacta actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac
     5881 accaataaac aaggcgtatc gaatcaatcg ctagatgaac tgaataagat gaagtcgtct
     5941 ttaattgatg atgttgccaa cgccatagga gtacctacgg cgcttattta tggtgaaaaa
     6001 gctgaactgg attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa
     6061 aaacttcaag acgaattggt ggcaaaaata attagtcgtc aagagtacaa aaacggggaa
     6121 cgtatagaag ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgat
     6181 aaaataacat ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat
     6241 gagttgccaa ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg
     6301 aagggaggtg agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga
     6361 aattcagaat ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg
     6421 tgatgatgat gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga
     6481 tattgtaatc aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa
     6541 ttatttaaaa gatcacccat caaatattac tgtagaagtt acagggcttg ccgcaagcgc
     6601 agcaacgttt attgtcgctg gtgccaatga tgtgattatg aatgttggga cttctttgat
     6661 gattcatgaa gcttctacat ttgcttgggg gaataaacag gacatccaaa aaacgttaaa
     6721 cgcattggaa acgatcgatg attctatctt agctatttat tcagataaga ctggccaatc
     6781 tgttgataag ttgcgtgaat ggatgaatga agaaaaatgg tttacagcag atgaagctgt
     6841 caaatatgga tttgccaatt ctgtgaagcg tgaagaacca caaaatgaac ctcaaaatat
     6901 tgcagcgatg attcaagatg ctgttgccgt tgcaatagcg aatttaaagg aacctgttac
     6961 taataaaatc gaacaagaat caaaacaaaa atcattaatc gcacgattgc gaaaaggaga
     7021 ataaagatat gttaaaaatt acagataaaa ctgcagatgc aaaaaaggca tttaatgcta
     7081 tttcttcgaa agaagatgca acacccgaac aaattaatgc tgctctagaa gaatatgtaa
     7141 ccgccattgc agaagatgcc ggcaaacagg tgcgtgctga atatgaagaa ttgaaaaatg
     7201 tgacggacaa ccgtgtatta gaggctcgtg gaattcctac actaacagca gaagaaacaa
     7261 agttttataa tgaagccgta aaatctggag gtttcgattc tgacgtagtt tggccagaaa
     7321 cgattttgga acgtgttttt gaaaatcttc aaaacgatca tcctatttta ggcctcatta
     7381 actttacacc aactgtaggt cgtgtcaaag taattagagc acgtcgtaaa ggtgttgctg
     7441 tttttggacc attgcataaa gaccttgaag gccaactaga tgctgaattt ggtgcaactg
     7501 aatttgttca acttgcgttg accgcatttt tcttaatttc taatgatacc ttagatttag
     7561 gaccacgatg gattgaccgt tttatcaatc tttctttaag tgaagcagta cgtgatgttt
     7621 gggctaaaaa aattattaca ggttctggta aagatgagcc aattggattg cttaaagatc
     7681 tagatggtgc ggtcacaagt ggtgtatatc cagataaagc tagcgcaggg acattaactt
     7741 ttgcgaaaga gaagattgtt cttgaacttg caggggtgat gaaaaaatta tctaaataca
     7801 catacaaagt agatggtgac gatgaaggtg aagtgaaata ccgttctgtt gctggacgag
     7861 tgtatctaat tgttaatcct gttgattact gggatatcgt agctagagtt acatttgcaa
     7921 atttaaacaa tgtgtatggg actacgatgc catttatcgc tgttgatcat atcatcgaat
     7981 ctgtagacgt accagaaagt aaatgtattg catacgttga taaagagtat gaagctactc
     8041 aatcaagagc tgaaaaagtc tatgtatata aagaaacttt tgcaatgaaa cgcgcaactt
     8101 tgtatgctgt cgacatgctt ggtaatggct atccaacaaa taatgatgca gcacaagtat
     8161 atgacttgga ctttacagcg ggggagtaat gtcccctgca attggtgaga taacaccgac
     8221 gactgatggg gcagtcatta atctttcgta aatggggtta gagtatgaac actgaagaat
     8281 ttataaaaga atataaggcc cgatttcgta ttttccatac ttccgaagat gaggatatcg
     8341 ggaaacagtt agagagcggg tttgccgata taaaatctct aatcggcagt tttgatcctt
     8401 ccacttatga gaaaggaaaa gaattagttt atgaacgtac tcggtatcta cggaacgaat
     8461 cactagagta cttttacgac aattttcaaa caatgattat ggatgcatca attgatctgg
     8521 caggtgctgg atatgccaat taaatcaaat tataagaagc ctaaaattgt agctggtgat
     8581 ctaaatacgc cagttacttt ttttgagttt caacctaata agggtccaga acctggtgat
     8641 gaagaaaaaa aggaactgta tttttgtaca gcactagttt acaacccatc aatgaaagat
     8701 cgtgacattc ttggcacaaa agaaggcgtt acgataaaaa tacgggatcc ccatcaatct
     8761 tatattccta ctaacaaaca taaagtaagg atattagatt atcgatatgg agataaagtt
     8821 tgggaaataa tcgatgtctc accggatttg gaagaaaatt caattgtaaa gatagtatta
     8881 gaattaatta cttaaatttg gtgataaatg atgagtaacg taaagtttga aggcgttcaa
     8941 gaaacaatcc aagctttaga aaaaaagtat ggagaaaaga aaactaagac attaacaaaa
     9001 aaagcaatca atgttggcgc tgaaaaagta gaaaaacagt tgcaaacgga catgaccgtt
     9061 tttaaagaca aaggttatac gatcgctgag gttgttcgca agaacgctac gtatagagat
     9121 tacaaggcag aagctgaaat cggttggaat ggtccacatc aacgatatag aatcattcat
     9181 ttgaatgaat ggggatatac acgaaagggc aaacaaatta gaccgagagg atttggtgta
     9241 ataacaaaat ctcttaagaa ttccgagctg ttgtactttg atgctgtagg agaggaggtt
     9301 aaaaagagtt tatgaaagat atgcttaata tcatttacga ggctctttgt ttgaatgagt
     9361 atattcattc aatgacctac aacgagaata ccgagcaata tcgtattaaa ttttatgagc
     9421 aacctgaaac cgccgataaa acaggtccaa tgatcacaat tcgtcctgta gatacaccta
     9481 acgaagcgta ccacggaagt aacaaagagc tttctgtgga gcatatgata cagattgacg
     9541 ttgaatcgaa atatagagca acatgcaagc agattcaata tgagataaaa aaagagatga
     9601 aaaaactagg ctttggccaa atttccggtc aagggttaga tgaatacttt actgaaacaa
     9661 accgctttgt agacgcgcgt cgttacgaag gaaatacaaa aatttacgat accgaatatt
     9721 agaaattaag acacgaaaat aacgtgtctt tttttattac ccaaaattag gaggaaaatt
     9781 ttatgacttt agtaggattc aaaaaaatga caatcggggt ctttgacagc acagggaaga
     9841 ttccagcagc aaatttatat gtaattgaag gtgaacaaaa caaaggggcg actgtatctg
     9901 ctgaaatcag tgggctatca aaagaaccat caaaagttta cggatcgaac atcgcttact
     9961 atgtctctca aaaaggaaca ggcgatgttt cagctacttt tggattgtta gatttaccaa
    10021 cagaagtaaa tgacaaaatt cttggttaca aaaccgatac aaacaaaatc agcttcttgg
    10081 gagaagatac tgagccacca tattgtgcaa ttttgatgga atcggaggat ttgaacggcg
    10141 atacagcaat gcttgcgatg ttcaaaggaa aattcagtcg tgaatctatt aatcttaaca
    10201 ctacgacaaa tgaagctttc gaaccagaag cagaagaata tgtattttca gcgattgcca
    10261 atgatgttga aggtgatgct aaaggccaaa cagtcgcaaa atatgttgga gacgaagaag
    10321 cttctattac tggtttgaaa gagatggtat ttcctgcagc gggggagtaa ctagccccgt
    10381 tgttggagca atcactccca ctattaatgg ggcaacagtt gaattaagtt aggaggataa
    10441 ctatgccaga tacatttaaa atctataaaa aaaatggtac tgaatttaca aaagtcgcag
    10501 agggtgaaag tccactttct attactggaa ttgcagcaaa tactcaagtt gctaaaggag
    10561 attatcaagc tacgcgtgtg gtcggaggtc aagaatcagt aaaagtagat attcctgcat
    10621 tcaccactct tccaatttca gttactggtg tgacgttgga taaaacgaca gctgaagttg
    10681 aacaaggcgg aaccttaaaa ttaactccta ctgtaacacc agcaaatgca acagacaaaa
    10741 caggaagctg gagcagttcg aatacagctg tagctacggt tactggaggc aacgttacag
    10801 taaaaacaga cgctgaagtt ggcggaacta cagaagtttc atttactact acggatggcg
    10861 gtaaagtagc aaaatgtact attacagtta ttgaaaaagc agcgggctag tcttcggact
    10921 agctttttta tttggaaaat aatggaggaa tctaaatggc aaaagtaaga attgaattga
    10981 aagaagaatc aggaaagaaa gtctatgaaa acatagacac aacagggaaa gattatcgaa
    11041 aagctttaga aacaatcaaa aagctaaatg aagacggagt aatgatttgg aatcgtttag
    11101 acatttattt ggattttgct gtaggtatct tcagggatag caaattgaca gctgaacaaa
    11161 ttcttgatgg cttaccatct gagaaggtaa tgagcacatt ggacgacatc ctaggagaag
    11221 taatggggat tgaaagtaat ccagacccag acgcaaaaaa gtaacacccg aagaagcaga
    11281 agaaatgtac ttaaacctat gcagagagtt agtgaaagcg ggatggtcac tatctgacat
    11341 agaagataat tcctttgaaa tgatgatgaa aattgcgtgc acttcgccta agaaagagaa
    11401 acaaaaagaa gttgatctta aagacttctt gaaatccatg tagagaggag gtaaacatat
    11461 ggcaaatgga aaacctttag gcaatatgat tatcaaacta gacttagaca gttcagcttt
    11521 ttcaaaaggt ctagccggag ctaaaaacgc tgttaaccat caaatgaaag caatgaaatc
    11581 acagatgcaa gtaatgaact cttcaggaaa ttctctcggt gctttacaag caaaatacaa
    11641 tggtttaggc ggagttctac aagcaaatga aaagcaagta gaattgctca ctaaggctta
    11701 taaggatagt tttgactcta acggaaacgc tactgcttcg actgctaaat acgctaatca
    11761 attgaatcaa gcaacagcta gatcagcaag ttacgaatct cagatgaaaa caactgttgg
    11821 acagattgct cggatgaaag tggaaacgga agggtttacg gggaaactaa aggctaattc
    11881 tgatgtattg attaattctg ggaaaaaaat tgaagccttt ggtaaaggag tttctgggat
    11941 aggatctgcc ctaactgttg gagtaacagc tccaatagtg gcaggcgcaa ctgctgttac
    12001 tagtgcagct atttcatggg aaagtgcttt tgcaggtgta aaaaagacaa atgatgaagt
    12061 tgtagactcg aatggaaacg tagtttattc ctatagcgac ttagagaaag gtctgcgtaa
    12121 tcttgccacc caattacctg gtagccatca agaaatagca aatgtagccg aagcagccgg
    12181 gcaattaggt ataaagactg aaaacgtagt tagcttcact aaaacgatga tagacatggg
    12241 tgaatcgact aatatgtcgg ctgaaactgc ggcaacttct ttagcacgat ttgctaatat
    12301 tacccaaatg tctcaaaaag actttgataa attaggatca gtcattgttg atctaggtaa
    12361 caattttgct acaaccgaat cagaaatcac tgaaatggga ttgcggttag cgggagctgg
    12421 taaacagatt ggaatgagcc aaggcgaaat tctaggattt gctactgctc tatcttctgt
    12481 tggtgtcgaa gctgaagcgg gtgggtctgc cttttcgaag gtcatgatcc agatgcaact
    12541 agctgtagaa aaaggatttg gcgcttttga tcagctgaaa cagatggcag aagaacaagg
    12601 tgttccgtgg gttaatttag taaatgctgt gagagacggt ggaaaatcgt taaaagcagt
    12661 atcagagcaa atgggattca cttcatctga tttaaagaaa atgtataaag aagcggacaa
    12721 ttcgaaaagt tcgcttgaaa attttgcgaa tgtggcaggt gttacaagtg aacaattcca
    12781 gaagttgttc aagagtgatc cttcagaagc aatcatcaag ttcattcaag ggctgaaaga
    12841 ttcagaaaaa catggtactt cagcaatcaa agttctggat gacatggata taaaagaagt
    12901 tcgactacgc gacagtttat tgagagctgc aaatgctagt gacatattca aaggagcaat
    12961 tgaaaaagga aacggcgcgt ggaaacaaaa tacagcttta acaaatgaag ctaataaaag
    13021 atacgaaaca actgaatcaa agctaaagat attgaaaaac gaagtgattg atacagcaat
    13081 cgatcttgga ggtccatttg tagacgcgtt gcgtgatggt ctaaatgtat cgaaaccatt
    13141 gataaaaaac ttagggaata tggcgaaagc tttctcagct atgagtgatg atcaacaaag
    13201 aaacattatc aaatgggttg gattagcagc agcagctggt ccagcgctga agcttttagg
    13261 aactggtact tcagtaatag gtaaagttac ttcaactact ggaagattga cgaaaagcct
    13321 tgttgattta gcagcgaaag cagcagaaaa gaaggctatg gatgcctttg catcgtcggt
    13381 aacaacagct ggtgctgcag ctgcaaagac agcaggagct ggaggtattg gcgggttagt
    13441 ccctatgatg ggaaaaacag ctagtgctgc aagtactgct gcgggcgcca gcggaatagg
    13501 cgcaatgaca acgtcactag gtttgttagg tcctgctcta cttggtattg ttggcgttgg
    13561 tggcgcactg gcagttggct atggagcatg gaaattattt ggtgaagaag cttggaattc
    13621 ctcgcaaaga gtaaagcaat ggggaacaga tgtcggtgag gagatagata gtactcttga
    13681 cggagtacag aagaatattg aaggtgcaaa tggtcaattc ggtcttctca aagagggttt
    13741 tactgttgat gatgcaaata atatggcggc taatttcgca accgctggaa catctttgga
    13801 aacatcttta acgggtagaa taaaggcatt agacgatgca ctaaaagggc tacctcaatc
    13861 agttcaggat gccatgaaag aagtagctga atcagaagaa gaaaccttag ggcaatctct
    13921 ttctaaaata aaagacaaca atgaagaaat aaaaaaaatc agagaaagag ccgcaaatca
    13981 gaacagagac ttgactgttt cagaactaca acagatacag gatctaatga aaggaacttc
    14041 tcaagcgtat gtggatacgc tcgatgtttc tgctaaagac agaaaaaaga tactatcagc
    14101 catgaatggt gatgttgcac aagctacaga agaagaagcg aagacgtggt tacaatcttt
    14161 agcgaaacaa aaagctgcaa ctaccgaaaa ttatagacaa atgcttgaag aaaaaaaggc
    14221 ggctttaaag gaaaagggag taagcaaaga attcattgat gcattagaag atgaaatgaa
    14281 tgattacatc aaaactacaa atcaaggatt cgatagccaa atagctgcta taacaaataa
    14341 atatccacaa ttgattaacg aagtgagttt atcaaatggt caattattta gcaaaacggc
    14401 agctgctatg gacaatactg gtcagtatac aaaagttttt ctcgaaagta atgaaaagat
    14461 tttagcccaa gcgaagaaaa catcggatga aatagcagat aatgccgaaa aaaatgcaga
    14521 acaacttggt atggtagctg atgaggctac gcatgctgga tcaacttgga atggcttagt
    14581 tttagacgaa aaaacaggaa aagtaaaaac taatgtacaa gaagtagtga atgaagcaac
    14641 gaaggatttc actaaatgga atgaattgaa acctgttgtt catgatgcga aactaacaac
    14701 gaatgcaaaa gatgtcattt ccatagcggc agtacaaaat ggctattggg atgatatgga
    14761 atggaaagaa aaagaagcat tattagaaga tgagtgttct aaaaatgtat acaaggcttt
    14821 agaaaatgct ggtaaatgga atgagttaga tattccttct aaagaagcaa tcctaacttc
    14881 aaatactcct gaagttatgg gtgaaactct catgaatttg ggattatggg atacttacaa
    14941 acttcaagtg aaagatttag atttgaatgc gtataaagta tacgatgcta tcgatggatc
    15001 agaagaaaaa ttaaatagtt gggctcaatt agataacccc acaaaagaat tgttattaga
    15061 taactcagat ttttctacaa aactttttaa ttccgaagaa atgtttaaac gttggaatca
    15121 gttatcatct aatgcaaagt ttctttcggc taataatact aaatttacag aggttctttt
    15181 atcatctgac agaatgtgga atcaatgggt aaaccttcct gataatgaga aaagattact
    15241 tgcagataac gaagatttag ctaccactgt tttatcttca gaagaaatat acggaagatg
    15301 gactcaatta ccagataatc aaaaatatat gttagctgac aatactgact tagcaaacaa
    15361 aatattcgct tcagaagaat catggaatgc atggaaagca attccagaca cagttaaaaa
    15421 cttgcttggt aataatattg atttactggc taaagtgaaa gacgggactg taagtgtaga
    15481 agaatataac aaagttatgc cacttcttaa aactctatat ggagataacg ttaatttaaa
    15541 agaggcaatc agtggggcta aaagctctat agagaattac aacaaaaata tttttcctag
    15601 tgataaaact gcagtaggac acgatgaggc ttcacaagct gctaaagatg catttgaagc
    15661 gttcaacatc ttccaaacaa aaataccaga taaaattact aaaactgtat cagctgactt
    15721 tataggtcct atgccgaaaa atgcgaaagg aacaaacttc catcctggcg gagcagctat
    15781 ggtaaacgat caaaaagggc ctacttatga agagcttata accttgccaa gcggagaaac
    15841 ttttatacct aaaggacgta atgtcgtttt agacttacca agaggttcga aagtactgaa
    15901 cgccactaaa actaaacgtc tagtgcctaa atatgctgat ggtataggaa acataacgac
    15961 ggtttcatct acgctaaact tagatgcttt aattttagct attaacgagc taacgacggt
    16021 attgagaact caactgatgg caggaacgac tgatgctaaa gatactaaac tatcagatga
    16081 aacaaaagtt gccgatccga ttattccgga cagtctttct gaaaaatcag atcagtatct
    16141 aggaattgga gctcaatggt taactaatct aatgaatggt tggaactcag cggttcctca
    16201 atatatgaat agtgaaatgg tttttattac aaactatctc aatgcgctaa aacttcaaaa
    16261 caatcctaat tatacgcaag gagcaacatg gaataagaac ttactgaatg gatggaatag
    16321 tttaactggg acctttattg ctacaatcaa ttcattttgt aatcaagcaa tggtcacgct
    16381 tagaaactac aacactccta tgtacaacaa tggccgaacg tggcagcaaa ataatcttaa
    16441 tggttggaat tcattgtatg gatcattcat agcccgtgta aatcagcttg gtaacgattc
    16501 gattaacaac cttcgttcta aaagtggtgg tttttacaat gcagggacat tcttgttgca
    16561 atctttaata aacggaatga attcgttagg taattctctt tctactacaa tgaacaatgt
    16621 cgctaataca atggttggtg gtataggcaa aggagttaat ggcgtcatat cgggagttaa
    16681 ctatgtgctt aaggaagtcg aatcaagtaa gaatatcgga aactggccaa ttccacaata
    16741 cgctaaggga actgatggac atcctggtgg acttgctatg attaatgatc aaaaaggcct
    16801 agttcacgaa gaatatgttc agatgccaga tggacgtagt tttattgcca aaggtagaga
    16861 tcttcttgtt aatttgccta aaggtgctca ggtcttaaat gcttctttaa caaagaaatt
    16921 aaaggaacgt ttaaatattc cacactacga aaaaggtatt ggcaatttag atattgttga
    16981 tttgttagat gatgaaaaaa gaatgttaga attcttaact agcaaagttg atttttcagg
    17041 cattaatgaa ccatggcttg atatgactaa atcaggaacg tcattaatgt ccaaagctgc
    17101 aaatacaatg cttcaatcaa aattaagtga attcttcact catggcaatt ttgatggtgc
    17161 agtaaatgcc aatggtgttt atcaatattt agttgatgtg gcacagaaag taatgggtaa
    17221 gttccccgga cttacagtaa cttctggata tcgagcagga gatgcctact atcacggaaa
    17281 acggcaagct atcgacttgg cttatccagg tatttctgga gatccgagat atacagcagc
    17341 agcaaattac gcttttgaaa aattcccttc aaaaattgcg tatgtcatta cgaatggacg
    17401 tgtacgtgac cgcatgggat tatctggaac aaggtcaagc ggacaatgga cgaattggcc
    17461 agatggcgat cacttcgatc atattcattt aaacggttcg atggggtctg gcgatatatt
    17521 caatggagga gttggaggaa gtggtgttgc acgctggcgt tcttatgtgt ctaaagcatt
    17581 gaaaatgaat ggattaccag ctacagcagc gtatataaat gcctggatgt cccaaatcca
    17641 aacggaatct ggtggtaatg agaaagcaat cggcggcaat gatgggctcg ccgaaggcaa
    17701 tgcaacagga ttacttcaaa cgaaacccgg aacttttgca gctaacgctt ttcctggtca
    17761 cggaaatatt atgaacggat ttgataacat gttagcagca atcaattatg ctaagaaacg
    17821 ttacggagtt gcaggtatgt tgcaagttat cgggaaagga catggatatg ccaatggcgg
    17881 cttgattacc aaagacggac tatatcgagc aggagaagga aataaaccag aaatggttat
    17941 tccattgaca agaaaaacaa gagctatcga acttatgggt caagcattag ccttcctttc
    18001 tggaagcaat aaaaaagaaa cgaaaagtga gattaattct gtagataata gacatatcct
    18061 acaagcgata gaaaaacagt ctcgtatgac aaatgatctg ctatcattat tactctcgtt
    18121 ttttaaaagt aacaatcgat cagataaaga actagcttta gatattcaga aaattttaat
    18181 caggaggaca tagtatttga ggacagtaat attaagaaat aaattgaatg aagaaattga
    18241 tttgtccaca gaagattatt ttgctactga aatgtcgaat ctaggttttg aagttacaaa
    18301 agagcatatt ggacaatggg ggagtttcag agaaatagga gagagtattg aattgtctga
    18361 atttcaatca tcagttatga tttctgttca cggatttaga gaacaacaat tatatggttc
    18421 attagttgaa tttctggcta atggaccttt tgaactagaa ttttcttttg attcagaaac
    18481 aatgatcagg aaatgtagtt tgaaatcatt atctaaaaca gaaattgatt cccaaagctc
    18541 attgctaaca gatactttga atctatattt tacttcaagc tggtattcta ttaagcgtga
    18601 aaaattaata cagaattcaa cttctctcaa tacacgtgga aaagtttact catttgttcg
    18661 accatatgtc tacaccaaaa atgtatggga aaagaaaggt gtatttaagt tcagtaataa
    18721 ttctgtatat ttgacaaata gtacagagcg aatgtctccg ttaaaaataa ggattattgg
    18781 agagtgctca aatccatatt gggaagttat ccaaaactct aaagtagtag cttcagatgg
    18841 ctattttatt aatatgaaag acacacaaat attagaagtg tctagtttgc tagaagacca
    18901 aacggcaatt ttaaaagatg taggtggagt tgaatcttct gtttatcaac aacaagattt
    18961 tactaaatca aacttcgtcc aagcgcctct aggagaattc agcgttgttt ttcatgttgg
    19021 acatgcaaat gtagagattg aactttatga ggagcgtgat ctattttgat tttagccatt
    19081 accctctttc accgagatat gaatttatat aacgaacgtt taatttcgac tgagttcgaa
    19141 tttggaacgg atgaaattgg agaagaagta agtaatttta ttacagataa atatgtccat
    19201 gtaaaatcag gagattttct actagcaaaa tttattccta gcgggaagat tgcttatttt
    19261 ggagtgatta gctcgcaaga agacaaaaaa ataaattgca aaggattact cagtttggcc
    19321 gatagcgaga taccgactgt aaaaatatca ggtgctaatt atgaagagca tattcgacga
    19381 ctgattgaat actacttatt gaacgatcca acaaaacaac taaaaaatat tttggaggta
    19441 aaaacagaaa gcgttacttc tcattcttat caagctgttg acacaaataa gagacgattg
    19501 aatgcatata taataaatgg gttcaaaaaa tataatgtta aatggtatgt caaagagatt
    19561 aaaggtaaaa aaatatatac tggtattcga gcagttgatg attcaattta tattaaagat
    19621 aattcatctg agtttaatga ctgggatgtt tttgtgcaat ctcctggacc agggaacgag
    19681 aacaaattat tgatcgtaga taaagcaatg aaagatatag aaaatccctt ggtattgtct
    19741 acgtggtatt tagatgacga aaataatctt acgcaagatt caacgaataa gaacattgca
    19801 ttgcctaccg ttaatttagt aaatatatat gatcaaactc aagaagatag accttcttat
    19861 gaagaggtag ctaaatcgga gcttaaaggc aacgcctatt cacatgaaat taaagttaat
    19921 gttgtgctaa atgctagaaa tatcaatgta gaaaatatcg aaacaggaat gttagcgact
    19981 attgtttaca aagagcagac ttacaaatct gttctaactg cctggcgaat atccagcagt
    20041 aaaaagaata tagaattgac atttggtaat gtccgaagtc gttttatgga ttattttgaa
    20101 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    20161 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    20221 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    20281 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    20341 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    20401 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    20461 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    20521 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    20581 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    20641 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    20701 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    20761 aaggagcgta taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    20821 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    20881 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    20941 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    21001 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    21061 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    21121 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    21181 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    21241 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    21301 acttcatcat agacgtgtta gaacgagtag atattaattc ggagcaagcg aaatactata
    21361 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    21421 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    21481 atgaatcaga taagcaagta aatgatcgta ttgataaggc aaataatcgt attgatcaaa
    21541 ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcattgatt
    21601 tcgggggata tgattatagc ggtaagccga atttaatgtc caaactaaaa tcgagcgatt
    21661 ttaacgttgg ttaccacggg tcactaactt cggataacga aaagctacat tttacttctg
    21721 atggtacagg aagcattatt atgtttacgc gtattaatac acctcagctt gctagtggga
    21781 aaacctatac tctgagtgcg aaagttcgat ttgatgaagg aactacagga gctattgata
    21841 aattacgttt ggtgtatcgt acatcaccag gagaaaagat attattggaa gcaaatagta
    21901 caaatattac aacagatgat gtagggaaag aaataacaat caaaggtaca gctaacgtta
    21961 attatcaaat cacaaattta gatcgatttt atatgagtat tagctttgtt gacagggata
    22021 aaataaatgg cggatttaag ttgtacgaca tcaaaatcga agaaggctca acagccacac
    22081 cgtaccagcc taacttacta gatgaccctt actgggtagg taaaacatcg ttgggtgaga
    22141 atattgcaaa taaaggtacg acgttcccaa ttaaatctag cgcgtacgaa atatataaag
    22201 gtaacacgga agaagagctt ataataggtc aaacgtatac tatcacgctt aaaggaacaa
    22261 aacccgcaag tcaaaccttt gtagcatata attattggaa cgttaatttt ggagaactaa
    22321 aaccagttga gggattgaca gacgtatggt ctctaacatt cacaccaacg aaacttgaac
    22381 cgggtttgcc taaagacctt cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc
    22441 aaattgactg gctcaaaatt gaaaaaggcg acacccgaac cccgaatatt agtcagttta
    22501 aatactttgg tgaaggattg aaagatagca acaatccgaa cgactacagc tgggacatca
    22561 cgcctgaata tactgaaaaa ggcttgaatg atacggttag tttgactgaa ccgcaatcca
    22621 tcgaaggatt gaaaaatttc gaagatggta ttcaatcaaa aggaaaatct gtattgacat
    22681 cagacgacaa caaatatgaa gtcgtaacct taacagttac aaacgggaat accggatcag
    22741 caaagcttta tcgtgaagga aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa
    22801 gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg ccaccaatta
    22861 gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct cagttatcta
    22921 ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatca gcttattcat
    22981 ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta caaatcaatc
    23041 aagccttacg gattcgagca aatcattgtg aatgacgaag aacatttacc ggaagaatgc
    23101 acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg ggagggaaat
    23161 aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga aaagtattgg
    23221 acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg gttcgttcag
    23281 atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac caatggatta
    23341 ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg tacgaggctc
    23401 ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta agcgatgtag
    23461 cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca caaatcaatt
    23521 caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt aaagagcaac
    23581 taaatccagc ttcaaaagga ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg
    23641 tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact atgcattaca
    23701 gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt gatgcgtttt
    23761 tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat gatagcagga
    23821 tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc tgcatctttc
    23881 tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct gactaaagat
    23941 ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa
    24001 agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa aactttgttt
    24061 agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa
    24121 ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca
    24181 cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg
    24241 tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa
    24301 aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat tgccaattaa
    24361 cggatttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag gtagctcaca
    24421 agtagcaaat aatcagtata ttttactgca tgaaacggct aatgaaacag caacaggacg
    24481 caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt atattgtggg
    24541 agacggcgga attgtttacc aagtcggaca acctggttat gtacagtacg gtgctggttc
    24601 atatgctaat gccaatagtc ctgtgcagat tgagttacaa cacacacatg ataaagcaac
    24661 ttttgaaaaa aactataaag catacgttga attggctaga gattcagcaa tgaaatatgg
    24721 tattccatta acattagaca cgccttataa ccaaccagga atcaaatcac atttatgggt
    24781 aactcaaaac atttggggcg atcacactga tccgtatgga tacttatcag aaatgggtgt
    24841 aagtaaggaa aaactggctt acgatttagc tcatggattt acagacgaaa acccaacgac
    24901 ttctgagaac aagcctgtca ttgatccaac ccgagcaggt gcagcaaatc caactttatc
    24961 agatggaacg aaccattctc acattgatca gtttggagaa atcgaaaatg cgaacttaca
    25021 cgtcgctgga tggcacatcg ctaactataa gtacgagtat attttcatta tggactacaa
    25081 tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagaccgg atgtaaatca
    25141 agcttataat acttctggaa atgttggcta tcatgtatct tttaacatgc gtaacttccc
    25201 taataagaaa gtctatgtca tgatgcgggc aacgaatgat ccagagggaa acactaaagg
    25261 cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata
    25321 gctcctcgtt gaggagcatt acataactat attgacaact ataaaaatca ttcgataaaa
    25381 tagtgatgtt atcgcatatc ttcactatca cccataatag tcacactcca agctatgcga
    25441 taacaggttt gttgccacac attctactgg ttgattgttt atggctttat gtggcaacaa
    25501 ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg tcgtaggttc
    25561 gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga attttttgaa
    25621 gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc ttttttatgt
    25681 atccttttat ggattaatga aaggatgttt cacatagtta tatttctgta tatttgaaaa
    25741 gttttacttt gatttttaaa tagaaagaca tttgggttat attgtgagat aataataaag
    25801 aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc tttaaactct
    25861 tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc
    25921 ttataatt
//
LOCUS       DAZKAY010000037        25447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000037 DAZKAY010000000
VERSION     DAZKAY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.037"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..160
                     /locus_tag="ACG8WP_001398"
                     /pseudo
     CDS             32..160
                     /locus_tag="ACG8WP_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            274..513
                     /locus_tag="ACG8WP_001399"
     CDS             274..513
                     /locus_tag="ACG8WP_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027708.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            625..819
                     /locus_tag="ACG8WP_001400"
     CDS             625..819
                     /locus_tag="ACG8WP_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027709.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            905..1576
                     /locus_tag="ACG8WP_001401"
     CDS             905..1576
                     /locus_tag="ACG8WP_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9027710.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1629..3605
                     /locus_tag="ACG8WP_001402"
     CDS             1629..3605
                     /locus_tag="ACG8WP_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9027711.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3620..4372
                     /locus_tag="ACG8WP_001403"
     CDS             3620..4372
                     /locus_tag="ACG8WP_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9027712.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4388..5143
                     /locus_tag="ACG8WP_001404"
     CDS             4388..5143
                     /locus_tag="ACG8WP_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9027713.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5156..5416
                     /locus_tag="ACG8WP_001405"
     CDS             5156..5416
                     /locus_tag="ACG8WP_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027714.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5413..7503
                     /locus_tag="ACG8WP_001406"
     CDS             5413..7503
                     /locus_tag="ACG8WP_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP9027715.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7530..7994
                     /locus_tag="ACG8WP_001407"
     CDS             7530..7994
                     /locus_tag="ACG8WP_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027716.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7991..8233
                     /locus_tag="ACG8WP_001408"
     CDS             7991..8233
                     /locus_tag="ACG8WP_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP9027717.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8251..8553
                     /locus_tag="ACG8WP_001409"
     CDS             8251..8553
                     /locus_tag="ACG8WP_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027718.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8623..10686
                     /locus_tag="ACG8WP_001410"
     CDS             8623..10686
                     /locus_tag="ACG8WP_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027719.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10686..13298
                     /locus_tag="ACG8WP_001411"
     CDS             10686..13298
                     /locus_tag="ACG8WP_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP9027720.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13295..15826
                     /locus_tag="ACG8WP_001412"
     CDS             13295..15826
                     /locus_tag="ACG8WP_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9027721.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15876..16208
                     /locus_tag="ACG8WP_001413"
     CDS             15876..16208
                     /locus_tag="ACG8WP_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027722.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16209..16802
                     /locus_tag="ACG8WP_001414"
     CDS             16209..16802
                     /locus_tag="ACG8WP_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027723.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16965..18989
                     /locus_tag="ACG8WP_001415"
     CDS             16965..18989
                     /locus_tag="ACG8WP_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP9027724.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18989..20188
                     /locus_tag="ACG8WP_001416"
     CDS             18989..20188
                     /locus_tag="ACG8WP_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9027725.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20204..20848
                     /locus_tag="ACG8WP_001417"
     CDS             20204..20848
                     /locus_tag="ACG8WP_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027726.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20859..21665
                     /locus_tag="ACG8WP_001418"
     CDS             20859..21665
                     /locus_tag="ACG8WP_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027727.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21687..21917
                     /locus_tag="ACG8WP_001419"
     CDS             21687..21917
                     /locus_tag="ACG8WP_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027728.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22431..22739
                     /locus_tag="ACG8WP_001420"
     CDS             22431..22739
                     /locus_tag="ACG8WP_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027729.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22741..23163
                     /locus_tag="ACG8WP_001421"
     CDS             22741..23163
                     /locus_tag="ACG8WP_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027730.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23183..24772
                     /gene="mobP2"
                     /locus_tag="ACG8WP_001422"
     CDS             23183..24772
                     /gene="mobP2"
                     /locus_tag="ACG8WP_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP9027731.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24784..25119
                     /locus_tag="ACG8WP_001423"
     CDS             24784..25119
                     /locus_tag="ACG8WP_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027732.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25260..>25447
                     /locus_tag="ACG8WP_001424"
     CDS             25260..>25447
                     /locus_tag="ACG8WP_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027733.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9499 a         3935 c         5233 g         6780 t
ORIGIN      
        1 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
       61 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      121 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      181 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      241 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      301 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      361 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      421 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      481 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      541 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      601 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      661 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      721 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      781 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      841 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
      901 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
      961 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1021 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1081 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1141 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1201 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1261 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1321 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1381 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1441 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1501 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1561 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1621 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1681 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1741 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1801 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1861 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     1921 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     1981 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2041 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2101 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2161 aaaaagcaaa accaatggtt gtgattgcgc caatgacaga taaaacagga tcaggatttt
     2221 taaaaaatat tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga
     2281 aatttggtga cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg
     2341 agttatacaa gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc
     2401 aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt
     2461 cggaagttgt agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag
     2521 acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa
     2581 gtgacttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa
     2641 atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga
     2701 ttcatattcc aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa
     2761 gtgaaacttc tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt
     2821 atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag
     2881 aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga
     2941 ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt
     3001 ataacatgta tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt
     3061 tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg
     3121 ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca
     3181 aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg
     3241 gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag
     3301 caaatgcagc gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg
     3361 aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag
     3421 agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa
     3481 acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa
     3541 tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag
     3601 cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa
     3661 atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga
     3721 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     3781 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     3841 tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa
     3901 aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg
     3961 taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt
     4021 accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     4081 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     4141 cttagaatca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     4201 caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     4261 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     4321 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     4381 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     4441 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     4501 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     4561 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     4621 tcacgttcaa aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga
     4681 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     4741 tgttaaggat ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact
     4801 atccagtgat caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac
     4861 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     4921 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     4981 ttctgataaa aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc
     5041 gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga
     5101 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     5161 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     5221 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     5281 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     5341 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     5401 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     5461 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     5521 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     5581 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     5641 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     5701 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     5761 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     5821 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     5881 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     5941 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     6001 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     6061 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     6121 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     6181 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     6241 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     6301 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     6361 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     6421 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     6481 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     6541 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     6601 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     6661 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     6721 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     6781 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     6841 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     6901 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     6961 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     7021 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     7081 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     7141 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     7201 acgctgaagt gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa
     7261 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     7321 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     7381 cctaacttgc cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca
     7441 gcggtcagct tactattact tgtgttagca acttttagtt atgcggttct gagatacatt
     7501 taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt aataatagta gttacttcct
     7561 tgattgccat gattgttatt ttagtcggat gtagtaatga aaaaaataaa caaactaatc
     7621 aagataatgg cgtattgaaa tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt
     7681 ataatttgaa aattatagac gagactacct gggaatactc tgagagtgta tggcatccag
     7741 atcctgtaca aataacagtc aagaggcaaa aagactataa aggtttagaa agatacaaaa
     7801 tagtagattc agctggtgtt agggaattta taaacaaatc tgattcttta tttattgttg
     7861 ttccctatga gaagaatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaaa
     7921 agcgaacgaa agagaaattg attcacgatg gatcacaatc caataaatat aagttacaaa
     7981 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
     8041 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
     8101 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
     8161 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
     8221 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
     8281 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
     8341 agtttaaaaa acaatctgat gatttttgct gctgctgcgg ccgttgcttc cattgttatt
     8401 gggggtcttt tcttcctatt tggacgaaga ggagctgaaa atgggaagat gcacgtaggc
     8461 tatgtattga tgggggtagg agttatttta gcagcagctt ctattgtggt ttcgttattt
     8521 ggattgtttg gacaaacacc tcaaggatta taagaaagta aaaaagtagg agtaagaagt
     8581 agtcgatact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgattgca
     8641 ggcaatacca aaacagataa agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt
     8701 acttcttttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
     8761 gtgttaggga aagtactgga acacgaaagt cgatcttctg ttaagttgac tctcacgtta
     8821 catgacacag aaggaaaaat agagaaagct ggtacagaat acgtttttgg cccactggat
     8881 tatcaaattt cagaaggttt ctatcctaca ttgatggatt taatccgcct tgatgtgttt
     8941 aaagaaacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa
     9001 gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa
     9061 gtaaatgaag aagtaccatg ggaacgacca atatttccaa agaaggagtc aaaagaaaaa
     9121 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
     9181 gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
     9241 gagacggccg aagtagaagt tgatccagtt gaagcgaatg aagagtcaac atggactgaa
     9301 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaata
     9361 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
     9421 tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc
     9481 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
     9541 cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat
     9601 ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg
     9661 acggaacagt cgtctaattg ggagaaagca aaagaggaac taaataaaga agaacagcga
     9721 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
     9781 tattccttgt gtgtagcaaa gatcaataat tttactcaga cgaatcaaga agcgttatcg
     9841 aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga attttaccga
     9901 ggtttgcgta cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta
     9961 tatcaaacaa cgactgaagt tcttatggaa aaatacgaaa agatccagca agaattacaa
    10021 gtgcaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    10081 gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa
    10141 caggatcttg cggaacaaca aatggcaata gaaaaacaac ggttaaacca agaaaaggaa
    10201 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagctcag
    10261 ttaaattatc tagcacaacc tcaaccaacg ccttcagtag ctgctgttca aaccccaatg
    10321 aacaaccaaa caaatctaac tccttcacca cagcgttcct ttaacggatg gatggtggga
    10381 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    10441 aatcaatctt cacaggctga ttctgtggcc acaacgacgt caatcaagga agaagcccaa
    10501 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    10561 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    10621 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    10681 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    10741 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    10801 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    10861 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    10921 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    10981 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    11041 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    11101 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    11161 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    11221 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    11281 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    11341 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    11401 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    11461 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    11521 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    11581 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    11641 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    11701 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    11761 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    11821 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    11881 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    11941 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    12001 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    12061 taggctataa aggattttgt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    12121 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    12181 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    12241 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggagaaaagt aacttattag
    12301 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    12361 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    12421 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    12481 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    12541 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    12601 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    12661 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    12721 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    12781 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
    12841 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    12901 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    12961 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    13021 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    13081 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    13141 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    13201 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    13261 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    13321 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    13381 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattta gttggatttg
    13441 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    13501 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    13561 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    13621 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    13681 atttatggcc atgatcttga tttttggtgg gagttcttta atcgatgaag ggttaaatat
    13741 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
    13801 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acattactag caactgaccc
    13861 gaataaagta gaatccctgc caacagacaa aagaaatgct ttaacagcga aaaattttaa
    13921 aacagcgaat atcaatggga ttattactcc taaacaagct aaagaaatgg cggataaaga
    13981 taagagtggt ttatcagatt tacaaaagga tcggttaaga gcgttacaat accgattgga
    14041 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    14101 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattatct tagctggtga
    14161 atcttctttg gcggtggcct atttatttat cctatttacg atcgttactt gcattattga
    14221 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggcca
    14281 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    14341 tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc atgcggcagg
    14401 aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg cgctattcaa
    14461 aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa acggcagtaa
    14521 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    14581 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    14641 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    14701 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    14761 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    14821 tgaagatctt acagaaaaag ccaaagacac cttagaagag aaattagacg gcacgagagc
    14881 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    14941 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag ttaatgcagc
    15001 agtaaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    15061 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    15121 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    15181 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    15241 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    15301 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    15361 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    15421 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    15481 agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc aatcaactgc
    15541 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    15601 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    15661 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    15721 atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga acaatgcatt
    15781 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    15841 aggcctttta tttatatgaa aaggagaaat caattatgga taaatatata aacatgaaaa
    15901 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    15961 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    16021 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    16081 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    16141 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    16201 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    16261 gaaaaccaga agagcgctct atcttggact tatttccgta taaatcctgt acagaagagg
    16321 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    16381 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    16441 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    16501 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    16561 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    16621 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    16681 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    16741 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    16801 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    16861 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    16921 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    16981 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    17041 atcgggaaat agaaatttta aaatccaaag ggtatgattt agatttttta aaacaagtga
    17101 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    17161 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    17221 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    17281 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    17341 cgactgaccg aaataaaagc gcagaagaat acctggaact agcggaatat gctcgtaaat
    17401 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    17461 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctaaaa gggatggatt
    17521 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    17581 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    17641 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    17701 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    17761 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    17821 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    17881 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    17941 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    18001 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    18061 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    18121 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    18181 ttacttcctc aaattcgcgt tcaaatacgg agacgcaagt cacaggtaaa gatccagaag
    18241 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    18301 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    18361 cgtatggcat gatttttgat gggcatagta cgattcgaca ccttgaccaa caacagttgg
    18421 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    18481 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    18541 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    18601 agtgccataa tgcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    18661 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    18721 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    18781 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    18841 tgggtaaatc aatgacgaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    18901 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    18961 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca
    19021 gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg gtgtttggta cttctgcagg
    19081 tttgattact atattgatca ctgtgtttgt gacccttttt ctaatacttg cggcaggttc
    19141 tattgataat tctgattcaa gtagtggtgg cgaagctttt acaggagaat attcggaagg
    19201 cttacccatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata
    19261 tgctgtaggt gcagcagtga agtataaatt attaccaagt gtgatccttt cgcaatacgg
    19321 ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaatt attttgggat
    19381 tacgtggttt gatgggtgtc tgtttcctaa aggaactgcg cgtggaattg gtggcattga
    19441 aggtggctgg tacatgaagt ttcccaactc aaaagctgcc ttctcctatt acggctttat
    19501 ggtagccaca caatcaaatt ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt
    19561 attgatctta ggacgaggag ggtatgccgc cgcaggaatt actgaagaca gtgcctatta
    19621 taaaaattgt atgtctatta ttaatacaaa taaattgatg gagtacgatg agtttgcgat
    19681 taaacactgg agagaaggcg gcggaactgg tggtacgatt acaggaagct ggggcaatcc
    19741 attcccagga agtagcttag ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa
    19801 tccaggagga gaattccgac ctaacgggtt ccatgatgga ctagactttg gttcagtcga
    19861 tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc
    19921 tggtatttca ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt
    19981 gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa
    20041 agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat
    20101 ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt
    20161 accatttttg aatagcagca aaaaatagat caggagaaaa aatatggaaa ataaaacctt
    20221 aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg
    20281 gcagcttaaa gaagaacaag agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc
    20341 gtctgataag ctcaaaaaaa attcttctga ggcacaggcg aagatcgaca agttaaatca
    20401 aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc
    20461 aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat
    20521 tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga
    20581 agtgatcgac aaattaagtc ctgcagacga tgatcaccaa catgacgact acggaagtta
    20641 ctcttttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca
    20701 aaattttgtc gtatttgttg attatacaat cggcaatcct caatttaaag atgtcaaacc
    20761 acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt
    20821 cacagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa
    20881 gacaacgttt taaaaaacga aaaagttatc ttagatggct ggggatcttt atgattctcg
    20941 gctttttgat tcttgctagt ccgatttaca cacataaaaa ggtagacata ccagatgaag
    21001 taagcacgaa cgcattttta aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag
    21061 cgaaccagca attagttttt gaatttgcga tcgacgatga agatcagagc ttattaaaag
    21121 aactagctaa tcttaattat cgtgtagaag taaagacagc taaaggggat tatcaagcaa
    21181 taaaagccaa agtgatcaag gtgtctgatg attattttgt agtgaagtta gcacatgtac
    21241 cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg
    21301 atatgcaaga accacaagat ctgatttatt acgttcacga agataaggtt aaagatcaag
    21361 tgaaagatga agattatgaa caacatgcga tcaattacaa agtgaaagga tacaaaaaag
    21421 aacaaaaagc agcacaaaaa caaatggaat ccttccaagc aaccattgat ttgaatgaag
    21481 aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata
    21541 ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg caaatgaaag
    21601 atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga
    21661 tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt
    21721 tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
    21781 gtcgagaagt gaatttatct aatacaacaa tatctgagtt gaagaatggt cgaaagaatt
    21841 tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta
    21901 cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct
    21961 tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac
    22021 gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga
    22081 tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt
    22141 tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta
    22201 aatacacaaa taaccttaat ttataaagag cataaggaaa cgtgggaaca ctttttaaaa
    22261 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataattgtgg gaacaaaata
    22321 tttaaaaata ggatagtgaa aggatagcaa aggatagtaa aaggatagaa gcaggatagc
    22381 gaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
    22441 gaataagaaa tgtagatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaactaa
    22501 gtaaccgaac gggtaataaa tggagtcgta acgattattt aaagctactc gttgaaaatg
    22561 attttgatcg tcctttgatg gaatataaac aagataaatt tgatcaatta ttaaatcagt
    22621 taatagataa attttctttt gttcaaagtc ataatacaaa agtattagaa gaatatattt
    22681 gtcagaataa tcaaattatc gaacttttaa ttgaacaaag tagaggagga gaagtttaac
    22741 atggccaaag ttatagacaa gcatatttat ttttatgaaa cacatataga tgttctagat
    22801 gaaattattg caactcatgc agaggttaaa aactatagtg aagccgttcg gtttctatgt
    22861 atgaattatg attcagaagt ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgca
    22921 atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga tctccatttg
    22981 aaagatacag ggattttaat tggtacaaat tcaactgttt accaagaagc aaaagagcgt
    23041 gtggaaggca agataaaagc aaatcaaacg aagaaatatt ctcctaagat aaagaaaaca
    23101 ataagcgaat cagtgactac aaaaagtgaa gtatttgatc caacaaggtt cttaaaacga
    23161 taaaaattag aaggagaatg tgatgagtaa atcacctgca gtcattctca cttgtcaatt
    23221 taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc
    23281 gctggaagaa aaggaacgac gtacgcccga ggaagaacag gaactgcgtc taatcaagaa
    23341 tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta agacgggtaa
    23401 agaattcagc gaaacgtcga atgaagcgca atcgttgatg aaacatggaa tcaattttga
    23461 caagttagaa gaacaagatt ttaccaaata tctttcttat atgacacgtc actatgcgtt
    23521 agaacaaaag aaagtactga cgcatagtga acaaaaagaa taccaacgct tgaaccaagc
    23581 aataaaaaaa tatcaaacag agccacacaa aaaagcttca ggaaatcttc ctggagtttt
    23641 ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaagc aaaccttcca
    23701 aagaggacaa caaaaaggct caatcattta tcaagatgtt gtctctcatg atcattcgta
    23761 tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag aaggtttaaa
    23821 agcagctgga agaaaaatga tggagacatt gttcgagaaa gagcaactga atgaaacagg
    23881 ctactggatg gcaacgattc accgaaatac caaacatatc catattcatt ttgcagttgt
    23941 tgaaaaaaaa aatacgcgta aaccatacac tgagattgaa aatggtgtga gttatatcgt
    24001 accgaaaggg aagagaaggc aagcaacatt agacgaaatg aagactaatt atgtacatga
    24061 gttagaacgt ttttcttatg aaaaaaatcg aatgggtatg gagaaacgaa atttattgac
    24121 acggaaaagt gatttgcgta acaccttaac caaagtagtg aaagagccag tacgttatga
    24181 tcgacacgca atgagcttat taactgaagt atataaaaac ttacctaaaa agcgttcgga
    24241 atggcattac ggggatgaaa atcgaaccaa actttcacca tacacaagag agaaactcga
    24301 tgagttaacc caatatgtgt taacaaaaga acctggatat caagaatata tacagatttc
    24361 aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga
    24421 tgcagaaaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca ttctaacaga
    24481 actgaagaaa caggatagtc aaataaaacc tttaatagaa aggatggatc agatgcaatc
    24541 tatttacaat gaacgatcct ttcgaacact taaacagttg ccaacgaatg aatacaaata
    24601 tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcaacg
    24661 gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa
    24721 agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt aggaggaata
    24781 gcaatgaaac aatatttaga tcaatggaaa gttatagaag gatcattaag agaagaacga
    24841 atcgaacaac ttcccgattg tttagaaaaa gagcatttgt ttcagataag agaaatgttg
    24901 ataaacgaac agtttgatcc aaatcaattt ttagtcgttg agtacccagc tactggtgtg
    24961 tactgttgta atcatgtgaa gggtgaaaaa tattttatca ttcaagaata tgagggcaag
    25021 ttagcgcctt attatactac ttgggaaatg aatgaagagg ggatcaataa cttcccgtgt
    25081 aaaagcattg aagagtctat ttcactcaca gaatgttagt tgttaagtga ataaaagaag
    25141 taaaagagtc ctgaacgtga ttgtccagga ctcttttttt tcactgaatt gttttccgcc
    25201 aattcattga gtggtgcgat ttccgtctac attatatcaa aagaaaggag gaagaagaaa
    25261 tggcttattc aacgattgga gttgttgttg aaaaaagcag agacaatctt gtttttgtga
    25321 cagaaattca aacaggcaga gcttttgtag tcacagacaa agcggcaaaa gcatatcaaa
    25381 gtggcgatat tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg gcagaagatt
    25441 atccatt
//
LOCUS       DAZKAY010000038        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000038 DAZKAY010000000
VERSION     DAZKAY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.038"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8WP_001425"
     CDS             complement(132..509)
                     /locus_tag="ACG8WP_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027734.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8WP_001426"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8WP_001426"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9027735.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8WP_001427"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8WP_001427"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9027736.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8WP_001428"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8WP_001428"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9027737.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8WP_001429"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8WP_001429"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9027738.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8WP_001430"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8WP_001430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9027739.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8WP_001431"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8WP_001431"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9027740.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8WP_001432"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8WP_001432"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9027741.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8WP_001433"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8WP_001433"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9027742.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8WP_001434"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8WP_001434"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9027743.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8WP_001435"
     CDS             10661..11575
                     /locus_tag="ACG8WP_001435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9027744.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8WP_001436"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8WP_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027745.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8WP_001437"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8WP_001437"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9027746.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8WP_001438"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8WP_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9027747.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8WP_001439"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8WP_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9027748.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8WP_001440"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8WP_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9027749.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8WP_001441"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8WP_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9027750.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8WP_001442"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8WP_001442"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9027751.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8WP_001443"
     CDS             21340..22764
                     /locus_tag="ACG8WP_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9027752.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8WP_001444"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8WP_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027753.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8WP_001445"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8WP_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9027754.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8WP_001446"
     CDS             24337..25314
                     /locus_tag="ACG8WP_001446"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9027755.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKAY010000039        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000039 DAZKAY010000000
VERSION     DAZKAY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.039"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8WP_001447"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8WP_001447"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9027756.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8WP_001448"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8WP_001448"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9027757.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8WP_001449"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8WP_001449"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9027758.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8WP_001450"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8WP_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9027759.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8WP_001451"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8WP_001451"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9027760.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8WP_001452"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8WP_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9027761.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8WP_001453"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8WP_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027762.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8WP_001454"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8WP_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027763.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8WP_001455"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8WP_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027764.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8WP_001456"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8WP_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9027765.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8WP_001457"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8WP_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9027766.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8WP_001458"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8WP_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9027767.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8WP_001459"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8WP_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9027768.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8WP_001460"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8WP_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027769.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8WP_001461"
     CDS             14353..15309
                     /locus_tag="ACG8WP_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9027770.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8WP_001462"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8WP_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9027771.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8WP_001463"
     CDS             16674..17276
                     /locus_tag="ACG8WP_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9027772.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8WP_001464"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8WP_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9027773.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8WP_001465"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8WP_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9027774.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8WP_001466"
     CDS             19701..20450
                     /locus_tag="ACG8WP_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9027775.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8WP_001467"
     CDS             20441..21226
                     /locus_tag="ACG8WP_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9027776.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8WP_001468"
     CDS             21195..21911
                     /locus_tag="ACG8WP_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9027777.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8WP_001469"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8WP_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9027778.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG8WP_001470"
     CDS             complement(23051..24052)
                     /locus_tag="ACG8WP_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9027779.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKAY010000040        24009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000040 DAZKAY010000000
VERSION     DAZKAY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.040"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..3408
                     /locus_tag="ACG8WP_001471"
     CDS             130..3408
                     /locus_tag="ACG8WP_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9027780.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3949..5856)
                     /locus_tag="ACG8WP_001472"
     CDS             complement(3949..5856)
                     /locus_tag="ACG8WP_001472"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9027781.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5986..6861
                     /locus_tag="ACG8WP_001473"
     CDS             5986..6861
                     /locus_tag="ACG8WP_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9027782.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6941..7915
                     /locus_tag="ACG8WP_001474"
     CDS             6941..7915
                     /locus_tag="ACG8WP_001474"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9027783.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8095..9240
                     /locus_tag="ACG8WP_001475"
     CDS             8095..9240
                     /locus_tag="ACG8WP_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9027784.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9377..9721
                     /locus_tag="ACG8WP_001476"
     CDS             9377..9721
                     /locus_tag="ACG8WP_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9027785.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9992..11278
                     /gene="efmA"
                     /locus_tag="ACG8WP_001477"
     CDS             9992..11278
                     /gene="efmA"
                     /locus_tag="ACG8WP_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9027786.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11416..12015)
                     /locus_tag="ACG8WP_001478"
     CDS             complement(11416..12015)
                     /locus_tag="ACG8WP_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9027787.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12419..13540
                     /gene="mnmA"
                     /locus_tag="ACG8WP_001479"
     CDS             12419..13540
                     /gene="mnmA"
                     /locus_tag="ACG8WP_001479"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9027788.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13786..15207
                     /gene="arcD"
                     /locus_tag="ACG8WP_001480"
     CDS             13786..15207
                     /gene="arcD"
                     /locus_tag="ACG8WP_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9027789.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15221..16339
                     /locus_tag="ACG8WP_001481"
     CDS             15221..16339
                     /locus_tag="ACG8WP_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9027790.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16399..17691)
                     /locus_tag="ACG8WP_001482"
     CDS             complement(16399..17691)
                     /locus_tag="ACG8WP_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9027791.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17841..18401
                     /locus_tag="ACG8WP_001483"
     CDS             17841..18401
                     /locus_tag="ACG8WP_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027792.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18530..18907
                     /locus_tag="ACG8WP_001484"
     CDS             18530..18907
                     /locus_tag="ACG8WP_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9027793.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18840..20912
                     /locus_tag="ACG8WP_001485"
     CDS             18840..20912
                     /locus_tag="ACG8WP_001485"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9027794.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21123..22070)
                     /locus_tag="ACG8WP_001486"
     CDS             complement(21123..22070)
                     /locus_tag="ACG8WP_001486"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9027795.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22316..22780
                     /locus_tag="ACG8WP_001487"
     CDS             22316..22780
                     /locus_tag="ACG8WP_001487"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9027796.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22842..23885)
                     /locus_tag="ACG8WP_001488"
     CDS             complement(22842..23885)
                     /locus_tag="ACG8WP_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9027797.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7801 a         4399 c         5054 g         6755 t
ORIGIN      
        1 aacaacttaa gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta
       61 ttgacagtaa aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga
      121 ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg
      181 ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg
      241 aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga
      301 actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat
      361 gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga
      421 gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct
      481 tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac ctattattat
      541 caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg
      601 acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat
      661 catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa
      721 tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg
      781 cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg
      841 ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg
      901 gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc
      961 acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca
     1021 tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa
     1081 aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg
     1141 acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg
     1201 caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag taatcattca
     1261 ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa
     1321 aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg
     1381 gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg
     1441 ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag
     1501 attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa
     1561 gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga
     1621 tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga
     1681 ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag
     1741 aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca
     1801 ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa
     1861 aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat
     1921 ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg
     1981 cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc
     2041 gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg
     2101 tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga
     2161 aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga
     2221 gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg
     2281 atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat
     2341 attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc
     2401 tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat
     2461 cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg
     2521 ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg acataccata
     2581 tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg
     2641 cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt
     2701 caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga
     2761 gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca
     2821 aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat
     2881 gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca
     2941 aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt
     3001 gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa
     3061 ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct
     3121 gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg
     3181 cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa
     3241 ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact
     3301 caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt
     3361 ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc
     3421 cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata
     3481 gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc
     3541 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa
     3601 tcagctgacc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     3661 tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca
     3721 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     3781 aaatttttct tactacttga aactaagcag ctttttcaca acctctaata catggtattc
     3841 cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc
     3901 ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg
     3961 cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata
     4021 gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc
     4081 gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca
     4141 attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca
     4201 gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc
     4261 gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg
     4321 tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc
     4381 gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt
     4441 agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac
     4501 gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc
     4561 tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa
     4621 aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc
     4681 gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat
     4741 gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata
     4801 aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata
     4861 tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc
     4921 tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct
     4981 gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat
     5041 ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta
     5101 cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt
     5161 tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat
     5221 tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa
     5281 ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa
     5341 taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga
     5401 tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt
     5461 ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa
     5521 cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc
     5581 gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag
     5641 ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag
     5701 cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc
     5761 gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc
     5821 ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt
     5881 cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa
     5941 ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg
     6001 cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt
     6061 aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct
     6121 ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat
     6181 tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat
     6241 gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc
     6301 gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt
     6361 tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca
     6421 gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt
     6481 cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa
     6541 ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag
     6601 aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca
     6661 aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca
     6721 tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat
     6781 tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac
     6841 ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg
     6901 ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga
     6961 tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa
     7021 agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc
     7081 catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc
     7141 acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag
     7201 tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc
     7261 gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc
     7321 aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc
     7381 tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac
     7441 gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct
     7501 ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga
     7561 gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt
     7621 gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga
     7681 agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa
     7741 tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc
     7801 agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca
     7861 tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa
     7921 aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt
     7981 ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc
     8041 tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat
     8101 gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt
     8161 gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt
     8221 ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa
     8281 gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc
     8341 ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa
     8401 catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat
     8461 ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa
     8521 gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc
     8581 aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa
     8641 gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct
     8701 gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat
     8761 atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat
     8821 ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa
     8881 gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac
     8941 gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt
     9001 cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct
     9061 atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca
     9121 atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga
     9181 aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa
     9241 ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa
     9301 cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag aaactaattt
     9361 tatcgaggtg aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag
     9421 ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac
     9481 aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg
     9541 attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac
     9601 agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga
     9661 taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg
     9721 atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt
     9781 gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt
     9841 ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat
     9901 ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct
     9961 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
    10021 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
    10081 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
    10141 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
    10201 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
    10261 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
    10321 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
    10381 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
    10441 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
    10501 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
    10561 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
    10621 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
    10681 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
    10741 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
    10801 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
    10861 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
    10921 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
    10981 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
    11041 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
    11101 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
    11161 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
    11221 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
    11281 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
    11341 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
    11401 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
    11461 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
    11521 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
    11581 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
    11641 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
    11701 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
    11761 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
    11821 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
    11881 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
    11941 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
    12001 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
    12061 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
    12121 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
    12181 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
    12241 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
    12301 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
    12361 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
    12421 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
    12481 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
    12541 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
    12601 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
    12661 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
    12721 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
    12781 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
    12841 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
    12901 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
    12961 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
    13021 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
    13081 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat
    13141 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
    13201 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
    13261 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
    13321 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
    13381 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac
    13441 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
    13501 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
    13561 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
    13621 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
    13681 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
    13741 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
    13801 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
    13861 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
    13921 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
    13981 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
    14041 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
    14101 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
    14161 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
    14221 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
    14281 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
    14341 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
    14401 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
    14461 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
    14521 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
    14581 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
    14641 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
    14701 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
    14761 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
    14821 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
    14881 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
    14941 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
    15001 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
    15061 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
    15121 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
    15181 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
    15241 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
    15301 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
    15361 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
    15421 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
    15481 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
    15541 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
    15601 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
    15661 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
    15721 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
    15781 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
    15841 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
    15901 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
    15961 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
    16021 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
    16081 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
    16141 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
    16201 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
    16261 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
    16321 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
    16381 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
    16441 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
    16501 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa
    16561 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
    16621 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
    16681 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
    16741 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
    16801 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
    16861 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
    16921 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
    16981 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
    17041 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
    17101 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
    17161 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
    17221 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
    17281 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
    17341 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
    17401 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
    17461 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
    17521 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
    17581 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
    17641 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
    17701 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
    17761 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
    17821 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
    17881 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
    17941 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
    18001 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
    18061 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
    18121 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
    18181 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
    18241 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
    18301 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
    18361 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
    18421 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
    18481 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
    18541 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
    18601 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
    18661 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
    18721 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
    18781 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
    18841 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
    18901 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
    18961 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
    19021 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
    19081 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
    19141 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
    19201 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
    19261 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
    19321 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
    19381 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
    19441 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
    19501 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
    19561 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
    19621 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
    19681 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
    19741 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
    19801 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
    19861 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
    19921 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    19981 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    20041 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    20101 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    20161 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    20221 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    20281 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    20341 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    20401 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    20461 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    20521 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    20581 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    20641 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    20701 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    20761 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    20821 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    20881 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    20941 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    21001 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    21061 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    21121 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    21181 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    21241 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    21301 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    21361 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    21421 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    21481 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    21541 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    21601 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    21661 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    21721 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    21781 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    21841 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    21901 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    21961 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    22021 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    22081 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    22141 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    22201 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    22261 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    22321 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    22381 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    22441 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    22501 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    22561 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    22621 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    22681 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    22741 acgccaattg ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca
    22801 ttgttccatg cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa
    22861 ataaatagta aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag
    22921 cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca
    22981 gtgatctcaa accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa
    23041 aacaagctgt tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat
    23101 tttcgttttt tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg
    23161 aatttttcaa acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc
    23221 atggcgaatt cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca
    23281 gattggagtg taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt
    23341 aatccaagaa aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg
    23401 atctgtcctt tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa
    23461 gaaccacaaa cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt
    23521 tttttaccaa tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca
    23581 attcctttta caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa
    23641 acagagaatt ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt
    23701 ttaaaaaaag tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac
    23761 tgtggcagat aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa
    23821 ccgggtaaga tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat
    23881 ttcatttgat cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac
    23941 taaaccagta ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg
    24001 tttcgaata
//
LOCUS       DAZKAY010000041        23825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000041 DAZKAY010000000
VERSION     DAZKAY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.041"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8WP_001489"
     CDS             complement(53..787)
                     /locus_tag="ACG8WP_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9027798.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8WP_001490"
     CDS             1114..1524
                     /locus_tag="ACG8WP_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9027799.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8WP_001491"
     CDS             1517..2218
                     /locus_tag="ACG8WP_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9027800.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8WP_001492"
     CDS             2218..3804
                     /locus_tag="ACG8WP_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027801.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8WP_001493"
     CDS             3823..4044
                     /locus_tag="ACG8WP_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9027802.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8WP_001494"
     CDS             4041..4802
                     /locus_tag="ACG8WP_001494"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9027803.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8WP_001495"
     CDS             5182..5691
                     /locus_tag="ACG8WP_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9027804.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8WP_001496"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8WP_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9027805.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8WP_001497"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8WP_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9027806.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8WP_001498"
     CDS             7318..8169
                     /locus_tag="ACG8WP_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9027807.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8WP_001499"
     CDS             8197..8832
                     /locus_tag="ACG8WP_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9027808.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8WP_001500"
     CDS             8852..9625
                     /locus_tag="ACG8WP_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9027809.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8WP_001501"
     CDS             10033..10830
                     /locus_tag="ACG8WP_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9027810.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8WP_001502"
     CDS             10808..11221
                     /locus_tag="ACG8WP_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9027811.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8WP_001503"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8WP_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9027812.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8WP_001504"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8WP_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9027813.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8WP_001505"
     CDS             15998..16558
                     /locus_tag="ACG8WP_001505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9027814.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8WP_001506"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8WP_001506"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9027815.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8WP_001507"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8WP_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9027816.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8WP_001508"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8WP_001508"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9027817.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8WP_001509"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8WP_001509"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9027818.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8WP_001510"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8WP_001510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9027819.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8WP_001511"
     CDS             21905..22483
                     /locus_tag="ACG8WP_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9027820.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8WP_001512"
     CDS             22535..23017
                     /locus_tag="ACG8WP_001512"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9027821.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8WP_001513"
     CDS             23134..23508
                     /locus_tag="ACG8WP_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027822.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5114 g         6643 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtagactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaag
//
LOCUS       DAZKAY010000042        23357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000042 DAZKAY010000000
VERSION     DAZKAY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.042"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..994
                     /locus_tag="ACG8WP_001514"
     CDS             137..994
                     /locus_tag="ACG8WP_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9027823.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="ACG8WP_001515"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="ACG8WP_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9027824.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="ACG8WP_001516"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="ACG8WP_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9027825.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="ACG8WP_001517"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="ACG8WP_001517"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9027826.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /gene="pstB"
                     /locus_tag="ACG8WP_001518"
     CDS             3750..4508
                     /gene="pstB"
                     /locus_tag="ACG8WP_001518"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9027827.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="ACG8WP_001519"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="ACG8WP_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9027828.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="ACG8WP_001520"
     CDS             5436..5735
                     /locus_tag="ACG8WP_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027829.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5776..6468
                     /locus_tag="ACG8WP_001521"
     CDS             5776..6468
                     /locus_tag="ACG8WP_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9027830.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="ACG8WP_001522"
     CDS             6465..7232
                     /locus_tag="ACG8WP_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9027831.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="ACG8WP_001523"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="ACG8WP_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9027832.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="ACG8WP_001524"
     CDS             9197..9517
                     /locus_tag="ACG8WP_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9027833.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="ACG8WP_001525"
     CDS             9517..9867
                     /locus_tag="ACG8WP_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9027834.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /gene="hprK"
                     /locus_tag="ACG8WP_001526"
     CDS             10034..10972
                     /gene="hprK"
                     /locus_tag="ACG8WP_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9027835.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /gene="lgt"
                     /locus_tag="ACG8WP_001527"
     CDS             10987..11817
                     /gene="lgt"
                     /locus_tag="ACG8WP_001527"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9027836.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="ACG8WP_001528"
     CDS             11861..12886
                     /locus_tag="ACG8WP_001528"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9027837.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="ACG8WP_001529"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="ACG8WP_001529"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9027838.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="ACG8WP_001530"
     CDS             13964..14404
                     /locus_tag="ACG8WP_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9027839.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="ACG8WP_001531"
     CDS             14407..15009
                     /locus_tag="ACG8WP_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9027840.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="ACG8WP_001532"
     CDS             complement(15077..16183)
                     /locus_tag="ACG8WP_001532"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9027841.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="ACG8WP_001533"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="ACG8WP_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9027842.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17730..20075)
                     /locus_tag="ACG8WP_001534"
     CDS             complement(17730..20075)
                     /locus_tag="ACG8WP_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9027843.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20354..21256
                     /locus_tag="ACG8WP_001535"
     CDS             20354..21256
                     /locus_tag="ACG8WP_001535"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9027844.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21469..23244
                     /locus_tag="ACG8WP_001536"
     CDS             21469..23244
                     /locus_tag="ACG8WP_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9027845.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6846 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    17701 agtcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    17761 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    17821 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    17881 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    17941 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    18001 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    18061 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    18121 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    18181 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    18241 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    18301 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    18361 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    18421 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    18481 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    18541 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    18601 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    18661 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    18721 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    18781 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    18841 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    18901 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    18961 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    19021 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    19081 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    19141 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    19201 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    19261 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    19321 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    19381 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    19441 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    19501 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    19561 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    19621 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    19681 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    19741 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    19801 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    19861 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    19921 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    19981 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    20041 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    20101 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    20161 atttcttttt taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    20221 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    20281 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    20341 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    20401 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    20461 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    20521 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    20581 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    20641 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    20701 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    20761 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    20821 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    20881 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    20941 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    21001 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    21061 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    21121 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    21181 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    21241 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    21301 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    21361 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    21421 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    21481 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    21541 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    21601 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    21661 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    21721 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    21781 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    21841 agtttctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    21901 acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    21961 atttattacc gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct
    22021 gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact
    22081 gtagtcgcgt taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa
    22141 gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt
    22201 tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa
    22261 aacgagacaa catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt
    22321 ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag
    22381 atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct
    22441 gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta
    22501 aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt
    22561 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta
    22621 gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca
    22681 ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat
    22741 gcctatgcat ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa
    22801 gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa ttggatcgat
    22861 agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc
    22921 atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata
    22981 ttacttatga tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct
    23041 ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg
    23101 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    23161 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg
    23221 aaaaaagctt tatcttccaa ataaaaacat actaagatta gaggagaaca tcattgacga
    23281 atgatgttct cctctatttt ctatttttag attatagtga agagtgaaag aaagttcgaa
    23341 caaagccttg aggacta
//
LOCUS       DAZKAY010000043        22762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000043 DAZKAY010000000
VERSION     DAZKAY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.043"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..924
                     /locus_tag="ACG8WP_001537"
     CDS             112..924
                     /locus_tag="ACG8WP_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9027846.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            921..1721
                     /locus_tag="ACG8WP_001538"
     CDS             921..1721
                     /locus_tag="ACG8WP_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9027847.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1882..3150
                     /gene="rseP"
                     /locus_tag="ACG8WP_001539"
     CDS             1882..3150
                     /gene="rseP"
                     /locus_tag="ACG8WP_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9027848.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3218..4927
                     /locus_tag="ACG8WP_001540"
     CDS             3218..4927
                     /locus_tag="ACG8WP_001540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9027849.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5139..9497
                     /locus_tag="ACG8WP_001541"
     CDS             5139..9497
                     /locus_tag="ACG8WP_001541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9027850.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9642..10115
                     /gene="rimP"
                     /locus_tag="ACG8WP_001542"
     CDS             9642..10115
                     /gene="rimP"
                     /locus_tag="ACG8WP_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9027851.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10138..11313
                     /gene="nusA"
                     /locus_tag="ACG8WP_001543"
     CDS             10138..11313
                     /gene="nusA"
                     /locus_tag="ACG8WP_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9027852.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11335..11628
                     /locus_tag="ACG8WP_001544"
     CDS             11335..11628
                     /locus_tag="ACG8WP_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9027853.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11625..11936
                     /locus_tag="ACG8WP_001545"
     CDS             11625..11936
                     /locus_tag="ACG8WP_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9027854.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11949..14255
                     /gene="infB"
                     /locus_tag="ACG8WP_001546"
     CDS             11949..14255
                     /gene="infB"
                     /locus_tag="ACG8WP_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9027855.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14282..14629
                     /gene="rbfA"
                     /locus_tag="ACG8WP_001547"
     CDS             14282..14629
                     /gene="rbfA"
                     /locus_tag="ACG8WP_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9027856.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14786..15242)
                     /locus_tag="ACG8WP_001548"
                     /pseudo
     CDS             complement(14786..15242)
                     /locus_tag="ACG8WP_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15407..17769
                     /locus_tag="ACG8WP_001549"
                     /pseudo
     CDS             15407..17769
                     /locus_tag="ACG8WP_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18137..19060
                     /gene="truB"
                     /locus_tag="ACG8WP_001550"
     CDS             18137..19060
                     /gene="truB"
                     /locus_tag="ACG8WP_001550"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9027857.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19064..20005
                     /gene="ribF"
                     /locus_tag="ACG8WP_001551"
     CDS             19064..20005
                     /gene="ribF"
                     /locus_tag="ACG8WP_001551"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9027858.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20357..21142
                     /locus_tag="ACG8WP_001552"
     CDS             20357..21142
                     /locus_tag="ACG8WP_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9027859.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21155..22006
                     /locus_tag="ACG8WP_001553"
     CDS             21155..22006
                     /locus_tag="ACG8WP_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9027860.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22130..22582)
                     /locus_tag="ACG8WP_001554"
     CDS             complement(22130..22582)
                     /locus_tag="ACG8WP_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9027861.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7735 a         3967 c         4966 g         6094 t
ORIGIN      
        1 aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac agttagatat
       61 tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag aatgttacgt
      121 ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt taataccgat
      181 ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg ggcgcaaaac
      241 cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca
      301 aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc gactgaaaac
      361 tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga cttctttgat
      421 acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac
      481 gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat
      541 actggaatgg tattgaattt tgctttgaat tatggaagca gagcagagat cgtgactgct
      601 gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga aatcgatgat
      661 gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca agatcctgag
      721 ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg gcagatcgca
      781 tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa
      841 gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa
      901 gatgagaaag gggatcagtc atgaaacaac gtgtggtcac agcagccgtg gcgctagtct
      961 tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca gcaatcttag
     1021 cagtaatagg agtttacgag ctgtttcgga tgaaaggatt gacactctta agttttgaag
     1081 gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga tggtttttct
     1141 ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg attcttttag
     1201 gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt cctgtgcttg
     1261 tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg
     1321 ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca tatatggttg
     1381 gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa acgattgaag
     1441 gtgcgctcgg tggtattggt agtgctgttg tagtcgcatt tctttattta ttgctatacc
     1501 cagcaaaaga actatttaat catggtatgt ttgtcatgct tctgatgacg attatttttt
     1561 cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat tatgatgtga
     1621 aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc gacagtttgc
     1681 tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac ctgcgtttct
     1741 ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac tattggtgtc
     1801 tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa
     1861 caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt ttttggtata
     1921 ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg aatcctcgtt
     1981 cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa agacgggaca
     2041 acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg caatggggat
     2101 gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc tgacggcatc
     2161 gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc gatggaattg
     2221 agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg agacgaaaca
     2281 gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga cggaacagaa
     2341 atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca gcgtatgctg
     2401 accaactttg ctggacccat gaataacttt attttggcaa tcctgttatt catcattctg
     2461 gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga gatcatgcct
     2521 aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga
     2581 aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc ggataaaaca
     2641 ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac accgaaatct
     2701 gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat gaatacaggt
     2761 tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt ggaaattttc
     2821 aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg acctgtcatg
     2881 atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat tagtttgatg
     2941 gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc cttagatggc
     3001 gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag tcaagaaaaa
     3061 gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt gctagttaca
     3121 tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt ataataacaa
     3181 acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa aaatgttgat
     3241 tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa tcttgctaag
     3301 agctggatat atccgccaag tatcagcggg tatttattca tatctgccat tagcaaatcg
     3361 tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga
     3421 aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc gttacgaaac
     3481 atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc
     3541 aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat ataagaaatt
     3601 acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg
     3661 tttgcttcgc ggaagagagt ttatcatgaa agatgcgtat tctttccatg caacggaaga
     3721 aagtttggac gagacatata aagattacga aaaagcctac accgaagtat tcaagcgttg
     3781 tggcttagaa tttcgctcga ttatcggaga cggtggcgca atgggcggga aagactcaaa
     3841 agaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga ctgaaagtga
     3901 ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat cgcatgaaac
     3961 acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg aagtagcaga
     4021 tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg cggatgaaca
     4081 acctgtcctt gttttggtac gcggagacca tgaagtcaat gacgtaaaac tgaaaaactt
     4141 tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat atctaggtgc
     4201 tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg
     4261 ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat tccactataa
     4321 aaatgtgaat ccagaacgcg atttcactgt actttcttat gaagatcttc gttttgtaca
     4381 agaaggagat ccttcaccag acaacaatgg tgttcttgcc tttactcgag gaatcgaaat
     4441 cggacatatc ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa ctgtactaga
     4501 cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg taagtcgttt
     4561 attatcagca atcgtagaac aacattcaga tgaacaagga atcaactggc caaaaggaat
     4621 tgcgccattt gatttgcata tcgtccaaat gaatctaaaa gacgaatacc aaacaaacct
     4681 gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg ttgatgacag
     4741 aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc caatcagaat
     4801 cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga aaactggcga
     4861 aatggttgaa gtacgtaaag aagaattggt gaatacttta ccaatcctct tgaaccaagg
     4921 cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt ttttgacaat
     4981 ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa ttttttaatg
     5041 taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga taagatgcga
     5101 tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa cgagatttat
     5161 ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat ccactgctta
     5221 ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc
     5281 tgcatttttc caaaattttg cctattatgc tatatcggtc attgactcag catctgacta
     5341 tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac cagacattcg
     5401 atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa tgcgatacac
     5461 cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct
     5521 tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg ccattagtag
     5581 aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa gtggatgagc
     5641 agcaagcgga acgagtattg aaactttttg aagaacgcaa acaagaacag gcagaagcct
     5701 ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag gaaagaaaag
     5761 agcaatcgcc agctcttgaa ggacccatcc acatccagct aggacgaaat attccagccg
     5821 atgaaccaac gacaccaatg atcagtatcg ttgaagaaga acgaagagtc acacttgagg
     5881 ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc ttgactttga
     5941 aaatcacaga ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg
     6001 aacaagtatt tgatgcgatc agtgtcggta gctggttaaa ggtacgagga agtattcaag
     6061 aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa gtcaaacata
     6121 cacctcgcaa agattatgct cctgaagggg aaaaacgagt agagcttcat gttcatagca
     6181 atatgagtac gatggatgca acaaacagca tctctgatct tgtggcacaa gcagggaaat
     6241 ggggacaccg agctatcgcc attacagacc acggtggtgc gcaagccttt ccggaagcac
     6301 atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag
     6361 atgatggtgt cccaattgct tataatgatg cacatgaagc attgagtgaa gccacatatg
     6421 tcgtatttga cgtggaaacg acaggattgt ctgcagtata tgacacgatc atcgagctag
     6481 ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt atcgatccag
     6541 gacatccttt gtcacgaaca acggtagatc tgactggtat cacggatgga atggttcgag
     6601 gatcaaagtc ggaagaagaa gtattgcgga tgttccttga attttcaaaa gatacaattt
     6661 tggtagccca caacgcggct tttgatatgg ggttcttgaa tactagctac gcaaggtatg
     6721 gtattcctga agcggccaat ccagtcattg atacgttgga attagctaga tatctgtatc
     6781 ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa atttggtgtc agcttggaac
     6841 agcatcaccg agcaatctat gatgctgaag caactggtca cttagcatgg atctttgtga
     6901 aagaggcaat ggacaatcat aacatgcttt atcatgatca gctaaatgag catatcggag
     6961 aaggagattc ttacaaaagg gctcgacctt tccatgtaac gattttagca aaaaatcagg
     7021 caggactgaa agatttgttc aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg
     7081 tcccacgaat accacgctcc caattgaaaa agatgcgtga aaatctattg atcggctcag
     7141 cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa
     7201 accgagcaaa attctacgat tatattgaag ttatgccaaa agcggtctat gcgcctttga
     7261 tcgagcaaga attggtaaaa aatgaacatg atttggaaga aattatccaa aatttggtag
     7321 aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg taatgtacat tatctgaacg
     7381 aagaagatgc catttaccga aaaattttga tcaattctat gggcggagcg aatccgttga
     7441 atcgtcatag tttgccggat gtccatttcc gaacaaccga tgagatgcta actgcatttc
     7501 actttttagg ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag
     7561 atatctgcga agaagtgatt ccagtcaaag atgaacttta tacaccgaag attccaggtt
     7621 cagaagatga aatatcagaa ttgagttaca caaaagcaaa acaaatgtac ggagatccgt
     7681 tacctgagat cattcaaaaa cgattaaaaa aagagttgaa ttctattaat ggtaatggct
     7741 tctcggttat ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa gatggctatc
     7801 tagtaggttc acgtggctct gttggctcta gctttgttgc gacgatgaca ggaatcactg
     7861 aagttaatcc gttagcccct cattattatt gtccagagtg ccaatattcc gagttttttg
     7921 aagatggtac gtatggttct ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg
     7981 cacgcttaaa caaagacggt catgatattc catttgagac atttttagga ttccatggag
     8041 acaaagtgcc cgatattgat ttgaacttct caggtgatta tcaagcagaa gcccataact
     8101 atacgaaagt attgtttggc gaagactatg tttaccgtgc cggaacgatt ggtacagtcg
     8161 ccgacaaaac agcatatggc tatgtaaaag gatacgaaag ggacaataat ctgcagtttc
     8221 gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc
     8281 agcatccagg ggggatcatc gttattcccg attatatgga tgtctatgat tttacgccga
     8341 tccaatatcc agcagacgat caaaattcgg aatggaaaac gactcatttt gattttcact
     8401 cgattcacga taatgtattg aaattagata ttctagggca tgatgaccca actgttattc
     8461 ggatgcttca agatttatca gggatcgatc cacaaacgat ccctacggat gacccggaag
     8521 tgatgcggat atttgccggt cctgaagtac ttggagtaag tcaggaacaa atttattcca
     8581 aaacagggac attaggtatt ccagaattcg gcactcgttt tgttcgaggg atgctagaag
     8641 aaacgcatcc tacaactttt gcagaactac tgcaaatttc cggactatct catggtacag
     8701 acgtatggtt aggaaatgcg gaagaattga ttcgacgagg agatgcgaca ctggcggaag
     8761 taatcggctg tcgtgatgat attatggttt atttgatcca tgctggatta gatagcggga
     8821 tggcatttaa aatcatggaa actgttcgta aaggacaatg gaacaagatc cctgatgaat
     8881 tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt gcccgactgg tatatcgatt
     8941 cttgttcaaa aatcaaatat atgtttccga aagcccatgc tgcagcttac gtattgatgg
     9001 ctcttcgtgt cgcttatttc aaggtttatt tccctatttt gtattactgt gcatactttt
     9061 ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac
     9121 aagcaatgaa agaaatcacg gacaaaggat tagatgcttc cgtaaaagaa aagaatcaac
     9181 tgacagtgct ggagttagcc aatgaaatgc tggaacgagg attcaaattt ggaatgattg
     9241 atttgtataa atcggatgcc gttaactttg ttattgaagg tgatacgttg atcgcaccat
     9301 ttagagctgt cccaagtttg ggaaccaacg tggcaaaaca aatcgttgaa gcaagaaaag
     9361 acggtccatt tttatccaaa gaagatcttg ccacccgcgg taaagtttca aaaacattga
     9421 tcgaatatat gaacgacaat ggcgtattga aagatctacc agatgaaaat caattatcgt
     9481 tattcgatat gctataagca tctgttttcc tgtctgcttg taattttaag cagagtatgg
     9541 tatagttctt ataggtaatg atactaactg tgagtgagcg gatttttcgc tcactctttt
     9601 taatggaatt acgcacttaa ttttttaagg aggcgaaact tttgagtagc gtcgttgaaa
     9661 cagtcacaga aatggtgaca ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg
     9721 aatttgtaaa agaaggaaaa agctggttct tacgagtatt tattgacaaa gaaggcggta
     9781 tcgatatcga agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt gatgcagcag
     9841 atccagatcc gattcctcaa gcttatttcc tggaggtatc ctccccggga gcagagcggc
     9901 cattgaaaaa agaaagcgac tatgaaaagg cacttggtga ttatgtccat gtctccttat
     9961 accaaccagt agatggcgaa aaacaatatg aaggatttct tcaatcattt gatgctgaac
    10021 agcttacatt gaaaatacgc ataaaaacac gagaaaaaga attgactttt gaccgaaaaa
    10081 atattgcaaa agctcgctta gctatccaat tttaatcacg gagaggaatc agaaaaaatg
    10141 agtaaagaaa tgttaaatgc gttagacgct ttagaagctg aaaaagggat ctcaaaagaa
    10201 atcgtgatcg atgctttaga agcagcattg gtttctgcat acaaacgtca ctatggccaa
    10261 gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa agatccatgt ctatgcggta
    10321 aaagaagtaa cggaagaagt gatggattca cagttggaag tctcattgaa agatgcactt
    10381 ttaatcaatc cagcatatga gatcggtgat acgatccgtt ttgaagtaac accaaaagat
    10441 tttgggcgga tcgctgcgca aacggctaaa caagttattt tacagcgtgt acgtgaagca
    10501 gaaagaacga ttatttataa cgaattcagt gcttatgaaa aagacatcat gcaaggaatc
    10561 gtcgaacgcc aggataaacg ttatatttat gtcaatcttg gtaaaattga ggcagttctt
    10621 tctaaacaag atcaaatgcc aaatgaattt tatcaaccac atgatcgaat caaagtttat
    10681 gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg cagccatcca
    10741 gacctattgc gccgcttatt tgagcaagaa gtaccagaag tctatgatgg aatcgttgaa
    10801 attgtcagca tcgcccgtga agctggcgat cgttcaaaag tggcagttcg ctcaaatgat
    10861 ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc
    10921 gtcaatgaat taaaaggcga aaacatggat atcgtcgaat ggaatgaaga tccagctgta
    10981 ttcattgcaa atgcattgaa tcctgcacaa gtcatggatg tgatcttcga tgagacaaat
    11041 ccaaaggcct gcacagttgt cgttccagac taccaattat cgctagcaat cggtaaacgc
    11101 ggacaaaacg cacgtttagc tgctaaattg actaaccata aaatcgatat caaatcagaa
    11161 tctgacatga cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat
    11221 gatgaagcaa tcgttcagtc agatttaaca gacgatgaat atgaaacaat tgcattcaac
    11281 aatgaaacgg tcgaagaaaa accagaagct taactttagt aaggaggcaa agagatgaaa
    11341 caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag
    11401 atggtccgaa tcacccgttc aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg
    11461 cctggacgag gagcgtatgt ctcacttgag ccggaagaag tacaacaagc ttgggataaa
    11521 aaaattctcg atcgagtact cgaaacaaca ttaacagatg agttttatca agaacttctt
    11581 gactatgtca ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat acagttaatc
    11641 ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc gggaaaacta atcacgggtg
    11701 aagaactgac cattgctgaa atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg
    11761 atgcaagcga gaatacaaga aaaaagataa aagataagag ttcatattat gaagttcctt
    11821 gctttgagct attttctgaa gcagaaatca cgcagatgat cggaaaacct cgcaaggtat
    11881 tcggaataat ggatgccgga tttgcgaaga agaccaagga gctaattgaa ggttaggaag
    11941 gtgattgcat gggcaacaaa agaatctatg aattagcaaa agaactcaaa aaatctagta
    12001 aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat atgggtgcga
    12061 ttactagcaa tgatgagaag aaattgcaac aagcgttcaa aaacccaagc acgaaacaac
    12121 aagctgcaca acaagcaagc agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa
    12181 aagaaacaac gaacaatcaa caaaaaaata aaaataaccg cagttatcaa gatcgcggtc
    12241 aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa
    12301 ataccagcag ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac cgtactaaca
    12361 ataacaacaa accaaacaat aacaatcgcg gcaaatttaa taataataac cgtaatcgtt
    12421 ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct gtaccaccac
    12481 gcaaattccg tgaattgcca gatgtattag agtatactga aggtatgaac gtagcagata
    12541 ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa aaaattattt atgcttggtg
    12601 tcatggtcaa tcaaaaccaa gcactggata aagatacgat tgaactgtta gcgacggact
    12661 atggcatgga accacaagaa aaagtccaag ttgacattgc tgatatcgat aaattcttcg
    12721 aggcagaaga agtcgatcca gataaattag tctcacgtcc gccagtcgta acgattatgg
    12781 gacacgtaga ccatgggaaa acaactttat tggatacatt acgccattca cgtgtaacaa
    12841 gcggcgaagc cggcggaatc acacagcata tcggtgccta ccagatcgat attgatggta
    12901 aaccaatcac tttcttggat acaccaggac atgcggcctt tacaagtatg cgtgcacgtg
    12961 gagcaagtat cacagatatc acgatcttag ttgttgctgc agatgatggc gtcatgccac
    13021 aaacagttga agcaatcaac catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa
    13081 ataaaattga taaaccaggt gcaaaccctc aacatgttat gcaagaatta agtgagtatg
    13141 agttaattcc tgaagcatgg ggtggagaaa cgatctttgt tgagatctca gcgaaattcg
    13201 gccaaaacat cgaagaattg ctggaaatga tcctgttagt cgcagaagta gaagatttaa
    13261 aagcagatcc aacacaacgc gcaatcggaa cagtgatcga agcacgtctt gataaaggta
    13321 aaggtcctgt gtcaacctta cttgtccaag aaggaacact tcatgttggt gacccaatcg
    13381 ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata
    13441 aagaagcagg acctgcaacg ccagtagaaa tcactggctt gaatgatgta cctcaagcag
    13501 gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg
    13561 cgaaacgtgc attattagaa aaccgtgctg ccacaagccg tgtgactttg gataacttat
    13621 ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa gcagatgtac
    13681 aaggttcagc agaagcactg gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg
    13741 taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag
    13801 ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag
    13861 ctgatactga agaagtcgat attcgtcttc accgtatcat ctataaagcc atcgaagaaa
    13921 tcgaaactgc gatgaaaggg atgctagacc ctgaatttga agaaaaaatc accggtcaaa
    13981 tgatcgttcg tgaaaccttc aaagtttcta aagttggtac gattgcaggt gcctatgtaa
    14041 cagaaggata tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct
    14101 atgaaggcca acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg
    14161 gttatgaatg tggtgcaatg attgaaaaat tcaatgacat caaagtagat gacgtaattg
    14221 aaggatttat catggaagaa gtcaaaaatg actaaaaact gataaaagga ggccaatcat
    14281 tatggctaac taccgtgacc gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat
    14341 tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta acgatcacag atgtgaaagt
    14401 tacgggggac ttacagcaag caacgatcta ttacagcatt ttatctgact tagcatcaga
    14461 taagaaaaca gcacaagatg gattgaataa agcatcagga ttgattcgtc gagagttagg
    14521 gcacaatcta agtatttata agacacctga attgttcttt gaacttgatg aatcagttgt
    14581 ctatggcaac catatcgatg aattgcttcg cgacttgaac aaagactaat caaaaactcc
    14641 cgcaaatcct gtgaaatcag gggttgcggg atttttttgt tttcattaag acgtattacc
    14701 taagagttcc atgactttag tcttgcggcg tgtcagctaa ccaaaaaaga gaaaaagaaa
    14761 agtaaaaaat tcgactttct tttttctagt agtatataaa tttttgcata tagataaaga
    14821 tcaagatacc gataacggaa gctagattgt aatctatttt agtgacagtc aggtatagcc
    14881 aagtaactag aagatcagtg gaacctgcac cataaaagtt tgagtttgtc ggtccgttga
    14941 cagttaaaag aaaaatcacg ttgaagttat tgatatttcc gattacgccg cttcgatttg
    15001 gtcacgatcc aaattttgga taatccctgt agaaattagc atagtagctg ggataccaat
    15061 tcacatattg acaaagataa cagagacttt tacataaaac ggatcaatca ggaaaggaat
    15121 tgctcgatcc agccccagtt ttccagcaat gtgttaactg gcccagcgtc attcagcaaa
    15181 ttatgcatga tgaggagcga aatgaactgc ggaacagcca ttgtgataac gacaagtttt
    15241 ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt atacctgttc
    15301 atttggctag atataaaatc caaacaaatg aacagaagtg cttattaggt ttcaattggg
    15361 agtatctgcg ctagaataag agtaataatg aaaaggagtg ttatgtatgg attattctag
    15421 taaagaatac tttgataaaa tgacggcttg gtggcgtgct gctaattatc tttctgttgg
    15481 gcaactttat cttaaagata accccttact gagacgtaca ctgaaacccg aagatgtcaa
    15541 aaaacatcca atcggtcatt ggggaacgat tcctggccaa aattttattt atgtccattt
    15601 gaaccgagtc atcaataaat atgatttgaa catgttttat attgaagggc caggacacgg
    15661 cggacaagtc atggtatcga atgcttattt agatggaagt tacacagaga tctatccaga
    15721 agtaacggaa gatgagacag gaatgcaaaa attattcaaa cgattttcat ttcctggagg
    15781 cattgcttct catgcagcac cagaaacacc aggttctatc catgaaggcg gagagcttgg
    15841 atactctctt tcacatgcag tcggagctgt attagacaat cctgaagtaa tctctgctgt
    15901 cgtgattggt gatggtgaag cagaaaccgg tcctttagct ggaagctggt tctcaaatgt
    15961 ctttatcaat ccagtgatag atggagctgt tttaccgatc cttcatttaa atggtgcaaa
    16021 aatagccaac ccaacgatct tggcacgtaa atcagatgga gaattagcaa attactttaa
    16081 cggacttggc tgggaacctt tctttattga aggaaatgat tcagaaaaat taaatcctgt
    16141 tatggctgaa aaaatggatc aagcaatcga gaaaattaaa tccatccaaa aagaagctcg
    16201 cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc
    16261 aaaaggatgg accggtccaa aagaatggga cggggaacca attgaaggaa ccttccgagc
    16321 acaccaagta ccgattcctg tagatcaaga acatatggat catgcagatg ctttactccg
    16381 ttggctgaaa tcctatgaac cagaaaaatt atttgatgca caaggacgta ttttagaaga
    16441 aatcagagaa atcgcgccta ctggtgacca aagaatggca aaaaatccta tcaccaatgg
    16501 tggaatcgat ccaagaccgc ttattatgcc agattggaaa aaatatactt tgcaatttga
    16561 aaaaccaggc tccgttaaag cagaagatat gaccgaactt ggtaaattcg ttcgcgagat
    16621 cattgagaaa aatccagaaa atttccgtat atttggacca gatgaaacaa aatcaaaccg
    16681 attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa
    16741 tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt caattatcgg aacaccaaga
    16801 tgaagggttc ttagaagggt acgttctgac tggacggcat ggttttttgc aagttacgaa
    16861 tcattcttgc gagtggttga ctctatgttg actcagcact tcaaatggat gcggaaatca
    16921 catgaccttt cttggcgaaa tgattatcct tcgctaaatc tcattgcaag ctcaacagtc
    16981 ttccagcaag atcacaatgg atatagccac caagatccag gaatattgac acatttggca
    17041 gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac attgctcgca
    17101 gtcatggata aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc ttccaaacat
    17161 ccgcgtgctc aattttactc agcagaagaa gcagccgtct tagtgaatga aggcttgaaa
    17221 atcattgatt gggcttcgac agcaaaagag gaggaacctg aattggtcat tgctgctgca
    17281 ggaacggaat ctaacttaga agcattagca gctgtaactt tgctgctgga agaattccca
    17341 aaactaaaaa ttcggttcat caatgtcgta gatttattga aattacgtca tccaagtcaa
    17401 gatccgcgtg ggttgagcga tgaagaattt gatcaatatt ttacaaaaga caaaccgatc
    17461 ttatttgctt tccacggtta tgaaacattg gtccgcacga ttttctttga tcgtcataac
    17521 catcatttaa tgattcatgg ctacaaagaa aatggagata tcacgacacc atttgacatg
    17581 cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag acgcagcttt aaaaatcaaa
    17641 ggaagtcaag cagaagattt tgcgaaaaaa atggatcaga aattacaaga acatcaaaac
    17701 tatattcgtg aaaatggtat tgatctgcca gaagtattgg attggaaatg gaaaaatctt
    17761 gaccaataaa atgataaaat cgatcccgca aaatagaatt ttttacattg attaagaaac
    17821 tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga atggccagtc ttttttttcg
    17881 tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc caatcaaatg
    17941 gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaagcataac atgatttcta
    18001 tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac cgttcattat
    18061 gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta aatcaaaaat
    18121 gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg gaatgacaag
    18181 tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg gacatggggg
    18241 tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag ccacaaaagt
    18301 tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc taggtttcag
    18361 tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag atatgtcttt
    18421 atctgaaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca cccagatacc
    18481 accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg cccgcaataa
    18541 tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc ggacaagtga
    18601 attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa
    18661 aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt accctgcgca
    18721 catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg ctctgacatt
    18781 agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc tgccaataga
    18841 aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc agaaagtaaa
    18901 aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt cagaagaaat
    18961 tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga aagaaaaaaa
    19021 caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata tcatcaagat
    19081 cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag tgctgggatt
    19141 tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg aaattgcaca
    19201 aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta ttgtctttaa
    19261 aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag aagaaaaaat
    19321 ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt ttgctcattt
    19381 agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat atgctgtttc
    19441 cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt ttcctgaata
    19501 tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat
    19561 cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag cagcaaaact
    19621 attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac gaggcagact
    19681 actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc ctaaagaagg
    19741 tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg gttcaattgg
    19801 acataatgat acattcggtg aaggacggga gctgactgta gagttgtata ttttggactt
    19861 taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt taagagatca
    19921 agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg aacaagcaac
    19981 agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt tatgtataac
    20041 tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg acaaaagtct
    20101 tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc ggaaataagc
    20161 cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga aataaaagga
    20221 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
    20281 ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt ttaggtatga
    20341 aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag taactggtgg
    20401 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
    20461 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
    20521 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
    20581 acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata atgctgggat
    20641 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tgttaaaagt
    20701 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaataa
    20761 taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat ggccaacgtt
    20821 tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg tagcaatgga
    20881 gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc gtacacctat
    20941 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
    21001 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
    21061 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
    21121 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
    21181 ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
    21241 tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc aattattcgc
    21301 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattttg
    21361 ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
    21421 gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct tcgcttttgg
    21481 agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc ttcttgtatt
    21541 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
    21601 aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat tgcctaaact
    21661 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
    21721 agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga gttggaaagc
    21781 aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt cagcaaatga
    21841 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
    21901 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
    21961 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
    22021 aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
    22081 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
    22141 gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag tttacgacat
    22201 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
    22261 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
    22321 attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg attggtgcat
    22381 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
    22441 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
    22501 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
    22561 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
    22621 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
    22681 tcaagtcaga aaattctaac ttttatttct ctaaagaaat tcgttctctc ctaaagtgat
    22741 attatattta aatatacctg aa
//
LOCUS       DAZKAY010000044        22742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000044 DAZKAY010000000
VERSION     DAZKAY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.044"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8WP_001555"
     CDS             131..3658
                     /locus_tag="ACG8WP_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9027862.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8WP_001556"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8WP_001556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9027863.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8WP_001557"
     CDS             7446..7787
                     /locus_tag="ACG8WP_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9027864.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8WP_001558"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8WP_001558"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9027865.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8WP_001559"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8WP_001559"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9027866.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8WP_001560"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8WP_001560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9027867.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG8WP_001561"
     CDS             12998..13735
                     /locus_tag="ACG8WP_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9027868.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG8WP_001562"
     CDS             13749..14564
                     /locus_tag="ACG8WP_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9027869.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG8WP_001563"
     CDS             14577..15224
                     /locus_tag="ACG8WP_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9027870.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG8WP_001564"
     CDS             15240..15896
                     /locus_tag="ACG8WP_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9027871.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG8WP_001565"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG8WP_001565"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9027872.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG8WP_001566"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG8WP_001566"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9027873.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG8WP_001567"
     CDS             18238..18756
                     /locus_tag="ACG8WP_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9027874.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8WP_001568"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8WP_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9027875.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="ACG8WP_001569"
     CDS             complement(19643..20563)
                     /locus_tag="ACG8WP_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9027876.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20689..21741
                     /locus_tag="ACG8WP_001570"
     CDS             20689..21741
                     /locus_tag="ACG8WP_001570"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9027877.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG8WP_001571"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG8WP_001571"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9027878.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7582 a         3959 c         4832 g         6369 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgac tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gatgtcaaac tattttttct taaattaaaa
    22741 aa
//
LOCUS       DAZKAY010000045        22736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000045 DAZKAY010000000
VERSION     DAZKAY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.045"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1959)
                     /locus_tag="ACG8WP_001572"
     CDS             complement(28..1959)
                     /locus_tag="ACG8WP_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9027879.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG8WP_001573"
     CDS             complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG8WP_001573"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9027880.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2888..3640)
                     /locus_tag="ACG8WP_001574"
     CDS             complement(2888..3640)
                     /locus_tag="ACG8WP_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9027881.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3800..4009)
                     /locus_tag="ACG8WP_001575"
     CDS             complement(3800..4009)
                     /locus_tag="ACG8WP_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027882.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4020..5347)
                     /locus_tag="ACG8WP_001576"
                     /pseudo
     CDS             complement(4020..5347)
                     /locus_tag="ACG8WP_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5483..5638
                     /locus_tag="ACG8WP_001577"
     CDS             5483..5638
                     /locus_tag="ACG8WP_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9027883.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG8WP_001578"
     CDS             complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG8WP_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9027884.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG8WP_001579"
     CDS             complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG8WP_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9027885.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG8WP_001580"
     CDS             complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG8WP_001580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9027886.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9322..10254)
                     /locus_tag="ACG8WP_001581"
     CDS             complement(9322..10254)
                     /locus_tag="ACG8WP_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027887.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10411..11070)
                     /locus_tag="ACG8WP_001582"
     CDS             complement(10411..11070)
                     /locus_tag="ACG8WP_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9027888.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG8WP_001583"
     CDS             complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG8WP_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9027889.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12111..12401)
                     /locus_tag="ACG8WP_001584"
     CDS             complement(12111..12401)
                     /locus_tag="ACG8WP_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027890.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12810..14615
                     /gene="glmS"
                     /locus_tag="ACG8WP_001585"
     CDS             12810..14615
                     /gene="glmS"
                     /locus_tag="ACG8WP_001585"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9027891.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG8WP_001586"
     CDS             complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG8WP_001586"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9027892.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16066..17235)
                     /locus_tag="ACG8WP_001587"
     CDS             complement(16066..17235)
                     /locus_tag="ACG8WP_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9027893.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG8WP_001588"
     CDS             complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG8WP_001588"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9027894.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG8WP_001589"
     CDS             complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG8WP_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9027895.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22199..22720
                     /locus_tag="ACG8WP_001590"
     CDS             22199..22720
                     /locus_tag="ACG8WP_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9027896.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRCLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6330 a         4939 c         4046 g         7421 t
ORIGIN      
        1 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
       61 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
      121 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
      181 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
      241 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
      301 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
      361 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
      421 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
      481 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
      541 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
      601 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
      661 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
      721 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
      781 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
      841 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
      901 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
      961 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
     1021 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
     1081 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
     1141 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
     1201 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
     1261 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
     1321 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
     1381 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
     1441 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
     1501 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
     1561 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
     1621 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
     1681 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
     1741 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
     1801 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
     1861 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
     1921 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
     1981 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
     2041 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
     2101 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
     2161 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
     2221 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
     2281 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
     2341 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
     2401 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
     2461 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
     2521 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
     2581 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
     2641 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
     2701 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
     2761 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
     2821 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
     2881 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
     2941 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
     3001 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
     3061 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
     3121 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
     3181 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
     3241 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
     3301 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
     3361 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
     3421 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
     3481 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
     3541 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
     3601 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
     3661 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
     3721 ttataaatca gtcaaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
     3781 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
     3841 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
     3901 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
     3961 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
     4021 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
     4081 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
     4141 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
     4201 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
     4261 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
     4321 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
     4381 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
     4441 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
     4501 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
     4561 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
     4621 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
     4681 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     4741 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     4801 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     4861 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     4921 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     4981 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     5041 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     5101 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     5161 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     5221 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccattc gcttatttcc
     5281 tgtttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
     5341 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
     5401 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
     5461 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
     5521 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
     5581 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
     5641 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
     5701 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
     5761 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
     5821 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
     5881 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
     5941 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
     6001 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
     6061 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
     6121 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
     6181 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
     6241 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
     6301 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
     6361 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
     6421 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
     6481 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
     6541 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
     6601 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
     6661 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
     6721 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
     6781 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
     6841 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
     6901 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
     6961 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
     7021 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
     7081 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
     7141 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
     7201 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
     7261 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
     7321 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
     7381 cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag
     7441 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
     7501 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
     7561 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
     7621 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
     7681 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
     7741 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
     7801 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
     7861 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
     7921 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
     7981 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
     8041 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
     8101 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
     8161 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
     8221 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
     8281 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
     8341 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
     8401 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
     8461 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
     8521 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
     8581 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
     8641 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
     8701 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
     8761 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
     8821 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
     8881 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
     8941 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
     9001 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
     9061 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
     9121 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
     9181 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
     9241 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
     9301 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
     9361 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
     9421 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
     9481 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
     9541 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
     9601 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
     9661 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
     9721 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
     9781 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
     9841 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
     9901 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
     9961 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    10021 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    10081 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    10141 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    10201 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    10261 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    10321 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    10381 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    10441 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    10501 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    10561 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    10621 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    10681 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    10741 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    10801 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    10861 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    10921 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    10981 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    11041 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    11101 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    11161 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    11221 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    11281 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    11341 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    11401 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    11461 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    11521 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    11581 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    11641 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    11701 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    11761 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    11821 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    11881 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    11941 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    12001 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    12061 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    12121 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    12181 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    12241 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    12301 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    12361 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    12421 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    12481 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    12541 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    12601 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    12661 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    12721 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    12781 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    12841 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    12901 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    12961 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    13021 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    13081 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    13141 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    13201 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    13261 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    13321 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    13381 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    13441 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    13501 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    13561 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    13621 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    13681 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    13741 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    13801 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    13861 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    13921 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    13981 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    14041 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    14101 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    14161 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    14221 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    14281 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    14341 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    14401 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    14461 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    14521 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    14581 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    14641 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    14701 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    14761 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    14821 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    14881 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    14941 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    15001 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    15061 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    15121 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    15181 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    15241 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    15301 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    15361 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    15421 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    15481 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    15541 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    15601 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    15661 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    15721 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    15781 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    15841 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    15901 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    15961 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    16021 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    16081 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    16141 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    16201 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    16261 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    16321 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    16381 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    16441 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    16501 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    16561 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    16621 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    16681 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    16741 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    16801 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    16861 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    16921 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    16981 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    17041 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    17101 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    17161 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    17221 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    17281 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    17341 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    17401 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    17461 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    17521 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    17581 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    17641 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    17701 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    17761 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    17821 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    17881 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    17941 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    18001 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    18061 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    18121 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    18181 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    18241 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    18301 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    18361 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    18421 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    18481 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    18541 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    18601 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    18661 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    18721 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    18781 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    18841 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    18901 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    18961 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    19021 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    19081 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    19141 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    19201 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    19261 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    19321 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    19381 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    19441 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    19501 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    19561 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    19621 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    19681 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    19741 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    19801 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    19861 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    19921 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    19981 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    20041 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    20101 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    20161 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    20221 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    20281 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    20341 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    20401 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    20461 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    20521 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    20581 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    20641 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    20701 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    20761 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    20821 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    20881 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    20941 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    21001 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    21061 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    21121 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    21181 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    21241 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    21301 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    21361 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    21421 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    21481 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    21541 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    21601 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    21661 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    21721 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    21781 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    21841 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    21901 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    21961 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    22021 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    22081 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    22141 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    22201 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    22261 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    22321 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    22381 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    22441 gtgtctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    22501 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    22561 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    22621 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    22681 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtg
//
LOCUS       DAZKAY010000046        22473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000046 DAZKAY010000000
VERSION     DAZKAY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.046"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..722
                     /locus_tag="ACG8WP_001591"
     CDS             51..722
                     /locus_tag="ACG8WP_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027897.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            781..1221
                     /locus_tag="ACG8WP_001592"
     CDS             781..1221
                     /locus_tag="ACG8WP_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027898.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1233..1901
                     /locus_tag="ACG8WP_001593"
     CDS             1233..1901
                     /locus_tag="ACG8WP_001593"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9027899.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1965..2588
                     /locus_tag="ACG8WP_001594"
     CDS             1965..2588
                     /locus_tag="ACG8WP_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9027900.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2644..3030
                     /locus_tag="ACG8WP_001595"
     CDS             2644..3030
                     /locus_tag="ACG8WP_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9027901.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3243..4634
                     /locus_tag="ACG8WP_001596"
     CDS             3243..4634
                     /locus_tag="ACG8WP_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9027902.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5245..5613
                     /locus_tag="ACG8WP_001597"
     CDS             5245..5613
                     /locus_tag="ACG8WP_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9027903.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5793..6734
                     /locus_tag="ACG8WP_001598"
     CDS             5793..6734
                     /locus_tag="ACG8WP_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9027904.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6803..7534
                     /locus_tag="ACG8WP_001599"
     CDS             6803..7534
                     /locus_tag="ACG8WP_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9027905.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7678..9858
                     /locus_tag="ACG8WP_001600"
     CDS             7678..9858
                     /locus_tag="ACG8WP_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9027906.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9878..10360
                     /locus_tag="ACG8WP_001601"
     CDS             9878..10360
                     /locus_tag="ACG8WP_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9027907.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10376..11137
                     /locus_tag="ACG8WP_001602"
     CDS             10376..11137
                     /locus_tag="ACG8WP_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9027908.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11268..12602
                     /gene="murC"
                     /locus_tag="ACG8WP_001603"
     CDS             11268..12602
                     /gene="murC"
                     /locus_tag="ACG8WP_001603"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9027909.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12909..13394
                     /locus_tag="ACG8WP_001604"
     CDS             12909..13394
                     /locus_tag="ACG8WP_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9027910.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13387..14070
                     /locus_tag="ACG8WP_001605"
     CDS             13387..14070
                     /locus_tag="ACG8WP_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9027911.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14196..16841
                     /gene="polA"
                     /locus_tag="ACG8WP_001606"
     CDS             14196..16841
                     /gene="polA"
                     /locus_tag="ACG8WP_001606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9027912.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHANTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16886..17722
                     /gene="mutM"
                     /locus_tag="ACG8WP_001607"
     CDS             16886..17722
                     /gene="mutM"
                     /locus_tag="ACG8WP_001607"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9027913.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17686..18315
                     /gene="coaE"
                     /locus_tag="ACG8WP_001608"
     CDS             17686..18315
                     /gene="coaE"
                     /locus_tag="ACG8WP_001608"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9027914.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18367..18699
                     /locus_tag="ACG8WP_001609"
     CDS             18367..18699
                     /locus_tag="ACG8WP_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9027915.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            19011..19502
                     /gene="nrdR"
                     /locus_tag="ACG8WP_001610"
     CDS             19011..19502
                     /gene="nrdR"
                     /locus_tag="ACG8WP_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9027916.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19525..20898
                     /locus_tag="ACG8WP_001611"
     CDS             19525..20898
                     /locus_tag="ACG8WP_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9027917.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20898..21824
                     /gene="dnaI"
                     /locus_tag="ACG8WP_001612"
     CDS             20898..21824
                     /gene="dnaI"
                     /locus_tag="ACG8WP_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9027918.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21953..22393
                     /locus_tag="ACG8WP_001613"
     CDS             21953..22393
                     /locus_tag="ACG8WP_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9027919.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7622 a         3875 c         4896 g         6080 t
ORIGIN      
        1 ttcttttcaa aatatgctat tttaaaaatg atgaaaaaag ggggagaaaa atgaattttt
       61 tagttgtttt aaaagatact ctgattgagc gatttacatt tctatatgtt caattgcagg
      121 aaaataaaac attgctgcat ttttcaccag agttccatct agaggggcta acattaggag
      181 aaatctatca agcagaaggg ctgtcggctg ttttgcactt ttttaaggta gaattggaat
      241 taccagtaaa agattatatc gagctttctc ttgaatcttg ggatcgagca gtggttgaac
      301 tgtttccaga aggtttaatg atcgatacaa acgatgggaa gatccatctg actgcagcag
      361 aactgcaaaa acaaatgcgt tatcaaatag atgacgaaaa ttctttttct atttttagac
      421 gtcagcaaaa gattctaaaa agctttttaa aagtgatcag taaaaaacgg aatctgatca
      481 aaacgacaca gctgttgaaa aaatatccag gtctgcttca cacgtctatt caatttcctc
      541 aattgattac gatgatccat cgttttatac gtatgcaaca gctaccatct aagaagctgt
      601 ttcttccatt gacggacaca tttcgagtag tcaacagaga agataaaaag aaagtgattg
      661 tgattgactt taccagaaat cggaatgtcc ttcaccaagt tgcaatggaa aaggccaatt
      721 aaggcttttt ctttttggat ttttttgtca taataaatat tagatgatgg aggagataat
      781 atgatgcaaa catggttaga aaggctaata cgatttcagc agaactatca caaacttatg
      841 cagggaagat atgcgaaatt tgatcagttg aatcgcggtc tattgatcgc tgcgcttgtc
      901 tttagtttgc taagtcgttg gttgccctat agaatcggac aattgttgtt catcgtcttt
      961 ttcggatgga tgatatttcg tttcctatca aaaaaaatct accccagact aaatgagaat
     1021 caacgttatt tgaaatatct tgatagagtc aaagaaaaat atcatcagat aaaaaacaaa
     1081 aagcaagtca aaatggatcg aaaaagttat actttttttg agtgcccgaa ttgtcatcaa
     1141 aagcagcgtg cacctaaagg aaaaggacga atacgtgtga catgcaaaac atgtggtata
     1201 aaattcgaaa cgaatgttta agggagaaaa gaatgaaatt agtattatta cgtcatggcg
     1261 aaagcgaagc aaattttgaa aattactgga caggatggct agatgtcgcc ttaacagaaa
     1321 aaggaaaaga acaagcagca gttgccggtc aaaaaatgct tgaagccgga cttcaattcg
     1381 atgccgttta tacctctgtt ttgaaacgtg cgattcttac cggacaagtc gcactagaag
     1441 aaatgggtca gttatggctt cctataataa aaacatggcg tctaaatgaa cggcattatg
     1501 gggatcttgt gggtaaaaac aaagataaga tgaaagaaat atacggaaaa gatcaagtaa
     1561 aaacatggcg cagaggatat acggaagttc cgccgttaac cgcagaaaat agatttgacc
     1621 gcagatatga tcacttggat ccgcatttga tcccatacgg agaaagtctg gaaatgacag
     1681 tgaatcggat cattccctta tggcaagatc atctagcacc aaaactaaaa gatcggcaag
     1741 atttactagt tgtgggtcat ggtaatagca tccgagcact gacaaaatat ttagaagatg
     1801 ttcctgaaga tcaaatggat acgatcgata tcccaaatgc acaaccaatc cagtatacat
     1861 tagcagatga tttgacaatt ctggacaaaa aaatacttta aagaacacct ttatctaaaa
     1921 gtgagagata gaaaaaagaa gcaaaggaat ggaacaaaag gaacatgaaa aattatatat
     1981 gggactttga tggtacattg tttgatacgt atcccgcaat ggtagatggt gcatggcaag
     2041 cactaaaaga ttttggtatc agtatggaca aaaaagaaat ctacttcaaa atgaaaaagt
     2101 attctactag ttatttgatc aatgaatcaa acttgaatgc cagagagttt aatgaattat
     2161 ttcaccgata tgaaaaagaa agtacggaag tttcccgtcc atttccagaa acgaaacaag
     2221 tactggaaat gctaaaagac aacggcggtc gtcatttcat cctgacccac cgtttgacag
     2281 aatcaacatg gggcctgtta aaagaacatc gattggcaca tctgattgag gaagtagtag
     2341 ggatcgatca ggactttcct cgtaaacctg atccagcatc tttaaattat ttgatcgata
     2401 cttttcattt agaaagaaca gacacgatga tgatcggcga tcgtcgttta gatatcgaag
     2461 cagggaaaaa tgcaggagtc gctacttgtc tttacgacat cgatcatttt ctgggtgaga
     2521 tcccggcaga ttatgtggta ggaaatctga atgaaatcct taccttgcag agcatcatta
     2581 aaaagtagta tcccctttca aaggagatat gttataattt tcataaagga gtgataaaat
     2641 tatatggaaa tcagaagaca aaaagaagtt gtagaagcat ttcattatga tatgagattg
     2701 ccgaaccaag aagtagaaac agatttgaaa gtaggcttct cgcctttaaa agaaacgatc
     2761 gaaaactatc cgaaagaaca tagcatcgta ggagcacgct tagagttccg tttagtgttt
     2821 gatacatatg ttttatctgg aagtgtgagt cagatcaatc atatcctcaa tcggaaaatt
     2881 gaaggtcaaa atgatgtcac acaagaagaa gtggacgagc tagtagcacc gttattcgat
     2941 atcgtacgcc gaatgtctta tgaggtgaca gagatcgcct tagatcagcc tggggtaaaa
     3001 ttgaattttc aatcagaaaa acaagaatag acagtaaaaa catacctgcg atagattaaa
     3061 gctatcacag gtatgttttt ttattactaa ttgtaagcaa aaagaatgtc atttcgataa
     3121 aaatcgtcta aaatataaaa gtcactgaaa aaaatttttt caggaggatg gttgaagaat
     3181 cattctcggg aacctttact tccttcgatg gacttgtcat taataatatg aggaggagaa
     3241 aaatgacaac actcatcaca gttttagtgg ttctagtttt tgcggcatta ttatatgtgt
     3301 tctatcgtat gcaaaagcgc catacaaaat tttcaacacg cgtctttaca gcattaggag
     3361 ttggtatcgt ctttggcggt ctgcttcaat tgatctttgg tgtagacgac aaagtcgtta
     3421 ctcaatcaat ggaatggatc gggattgtag gtcaaggata tgtatcattc ttgcaaatgc
     3481 tagttatgcc tttagtgttt atttcgatca ttggtgcatt cacaaaaatg aaagtttctg
     3541 aaaagctagg gaaaattagt gcgactgtat tgacttcttt acttggaaca acagctattg
     3601 cagcattgat cggaattggt tcaacaatgc tgttcggact ccaaggagct aaattcacac
     3661 aaggagctgc agaaacagca cgaatctctg aactggcaac acgtcaggaa acagttgaaa
     3721 acttatcgat tccacagcag attttatcct ttatccctaa aaacgttttt gctgatttag
     3781 caggtagtcg tgcaacaagt acgattggag tcgttatttt cgcggcattt gtcggaatcg
     3841 cttatctagg cgtgaaaaga aaagcgccaa aagaagcagc attcttttca gatttagtcg
     3901 acagtctgta taaaatcatt atgcggatcg tgacgcttgt tttgcgtttg acaccttatg
     3961 gagtatttgc tttgatgaca aacgttttgg caacaagtga ttttgcagct atcgtcaatt
     4021 taggtaaatt tgtcgttgct tcttacgtag cattgcttgc tgttttgatt gtccactcac
     4081 tgattttgat tggtgcaaaa gtcaatccaa tcaactattt caaaaaagcc tttccggtat
     4141 tgagctttgc gtttacttca cgttcaagtg ctggagcttt gccattgaat atcgagactc
     4201 agacaaaagc tttaggagta gatgatgcaa cagctaattt ctcagcaagt tttggtttgt
     4261 ctattggaca aaacggctgt gctggcgttt atccagctat gttggcaacg atcgtcgcac
     4321 cagcagctgg agtgaatgtc tttagtttgg aatttatttt gatgcttgtg gcaattgtaa
     4381 caatcagttc ttttggtgta gctggagtcg gtgggggcgc aacatttgct tcactgatcg
     4441 ttttaggggc aatgaacttg ccagtagcaa tcgttgggtt agttatctct gttgaaccat
     4501 taatcgatat ggcacgtaca gcagtaaacg tcagcggtag catggtagct ggtatcgtga
     4561 caagtgcacg catcaaagat ttagatcgcg atgtcatcaa tgatgattct aaagtgatcg
     4621 atgtgaatgc atagtcaaaa agaggttgtg atagaagtgg tcagctttaa gaaataagaa
     4681 gaagtttcct aaaaatagtt tctcaaattt ttgtgaaatt tcggcttatt tccgagaagc
     4741 tacttctgaa acaccgtgaa taagaggttg tgaaaaagct gccctgcatc aagtaataag
     4801 aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat
     4861 gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc tgccgtttca ctctgagtca
     4921 caaagagcaa tttcaaaaaa tagcttctca tgtttgttga atattgtgtc ttgtgaccga
     4981 ggagttgtca ggagaacacc gtttaccaga ggttgtgact tgtttttagt cacgacctct
     5041 ttttttattt attccatata aatacacatt atttattccg aaaagtgaaa gcgcaaataa
     5101 caaaaacaac caacttattt attattaaag aaaagcgtat ttaagtagga cgattctgtt
     5161 gagagctttc gtagattttc ccattttcct atggtaaact gacaaagttg agaatgttag
     5221 aacagatcgt ttaggaggaa acagatgttt acgaatgaaa taaaaatcat gctttatgtg
     5281 gacgatgtag aaaaaaatgc aacgttttgg agagcagttg gctttgcgga aaaagatcgt
     5341 caagaaatgg atgggacgct tataattgaa gtaggagttg aggacagtca aacctcattt
     5401 ctgttatatg atcgagcatt tgttgagtcc cattcacctg gcacagctac tagtgcccca
     5461 tctttgatgt tttacagtaa agatatcttt ggactatata aaaaaatgca gacgcaatca
     5521 gtcacattag gtgatatggt acagcttgga gaagaatatg tctttaactt tgctgatccc
     5581 gatggcaact actttgcggt tacaggtaaa taatagaaat aaagactttg tttttctgtg
     5641 cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa caaaaaactt
     5701 tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg
     5761 tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg ctttattaca
     5821 attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat
     5881 ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt actgtgtctg
     5941 gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct cgatttgctg
     6001 atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg tttgtaagat
     6061 accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac caagtgacag
     6121 gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag acagtggtga
     6181 ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct gtacgcccag
     6241 atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa gaatttatgg
     6301 aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt tctggttggc
     6361 ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct acttatggta
     6421 aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt gatgaacgga
     6481 tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa
     6541 caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag attcttgctt
     6601 ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg aaacatccgc
     6661 gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg cggtttacag
     6721 ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat gcgctatact
     6781 agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg atgtccatgg
     6841 caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc atcagcttgt
     6901 tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa
     6961 gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag aatatttttt
     7021 gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg gtggaaaaga
     7081 aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa ctgaaatctc
     7141 gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc gccccttgta
     7201 ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa agaaagactg
     7261 gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg aagggaaaaa
     7321 caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc atggagatat
     7381 gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg gggctgtatt
     7441 cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag atattgaata
     7501 tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga aggagctgtg
     7561 actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt agtttaaccc
     7621 aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg cattttaatg
     7681 acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc agactatcgt
     7741 cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt tccttttatc
     7801 gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg tgaaatcgaa
     7861 gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc
     7921 gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg
     7981 caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa agcaacgatt
     8041 gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac agatggagag
     8101 gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc tgccgaagaa
     8161 gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc aaaatttcgg
     8221 acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt caaggatgag
     8281 caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc cgtccataaa
     8341 atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat tctgaaagta
     8401 ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat agtcaaaaat
     8461 cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta taaacgcttt
     8521 attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc agatgagcaa
     8581 gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt
     8641 aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc agtcgtagat
     8701 gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc agcgcaagca
     8761 aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca agtagatatg
     8821 gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc tgagcagttg
     8881 aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc ttctgtttac
     8941 tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga aagaagtgct
     9001 gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa
     9061 gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc tgaacaattg
     9121 gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg
     9181 cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat tgtcatttat
     9241 cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc acgcttagga
     9301 ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa aaacattttg
     9361 gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt aacttcagtc
     9421 caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac acgattatcc
     9481 gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga tattttagct
     9541 gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt attacgtaca
     9601 gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac ggtccgtaat
     9661 gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact tgtccatata
     9721 tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt aggagatatc
     9781 gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt gacgatgctt
     9841 tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag aactgcagca
     9901 gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt
     9961 caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc atttagattt
    10021 taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca tcaaacatga
    10081 actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag atatggattt
    10141 caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga cgaagaaaca
    10201 gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc gcaagagaag
    10261 aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat ggattgagac
    10321 aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa ggcctttgga
    10381 aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt tagccgcacc
    10441 tgaagaggga aattttgagg aactgacggc acctacactt attttagctg gaaactgtct
    10501 tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag atcaattgtt
    10561 tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg aaaagcaagg
    10621 atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc taaaagaagt
    10681 ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt ctggagagaa
    10741 cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa aaatcttatt
    10801 gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag tatggcaaaa
    10861 tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc acttttctga
    10921 agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat atttccatgc
    10981 gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg gtccgaaagg
    11041 aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt acaccagaat
    11101 taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag cgaatcctcc
    11161 tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg agcgtgttga
    11221 atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg gaaaatcaaa
    11281 ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg gctcttgttt
    11341 tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc tttacacaaa
    11401 gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat gtaaaaccag
    11461 ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc cagcgagcaa
    11521 aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt atccaaaatt
    11581 atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga ctgctctctc
    11641 atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca ggacatggtg
    11701 atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat ttccttgcct
    11761 attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat tattatacaa
    11821 gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa aaagctattt
    11881 ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg atttattatt
    11941 atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca acttctggtt
    12001 ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta ccagcattcg
    12061 gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc gaaaaactgg
    12121 atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt cgattcagtg
    12181 aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct gcagagatca
    12241 aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt gcagttttcc
    12301 aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa gcacttgatt
    12361 tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa caaggaaacg
    12421 ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc aaagaaaata
    12481 atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt gctggagatg
    12541 ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa aatgtactgt
    12601 aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca aaaatcttgc
    12661 caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat ctgcggtaat
    12721 aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct tattatttga
    12781 tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat caagggagca
    12841 gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata aactggaggg
    12901 tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat gaaatcgagg
    12961 aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg ctgtatttgg
    13021 gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa aaaacagaat
    13081 atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt gcaatttcaa
    13141 agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt attgaaccgg
    13201 ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat aagatgaagg
    13261 atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg ttggatgctg
    13321 tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa agtgaaggtg
    13381 aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta tgcaaaagtt
    13441 tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt ggctctttgg
    13501 gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt tgtgggtctg
    13561 tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa gaatccgagc
    13621 ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt agcggttact
    13681 cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac tttgatgttc
    13741 atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt tggaagagca
    13801 ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt cattttgaga
    13861 agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc agggattaca
    13921 gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac agcacatagt
    13981 ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt gttcttggcg
    14041 tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag ttggggctat
    14101 accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat tctttgttag
    14161 aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa ttattactcg
    14221 ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct cttgaacgtt
    14281 tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg atgtttgaaa
    14341 atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca ggaaaaacga
    14401 cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca cctggcgaat
    14461 tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta cagtattacg
    14521 aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa gtagacaaag
    14581 atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta gctagtaaag
    14641 aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat acaccagaac
    14701 atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg aagggactag
    14761 ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa acagctatca
    14821 agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat gaaatgaagc
    14881 aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta tcgaaaaaat
    14941 tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg aagtatgaag
    15001 ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat cagttccttt
    15061 ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt gaagtcgttc
    15121 atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa atgatgggcg
    15181 acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag aaaatttatg
    15241 tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc actgatccga
    15301 ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac cgctatactg
    15361 gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc gatacgaatg
    15421 aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat atccaatcag
    15481 atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag gaactctttt
    15541 ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa ttgagccaag
    15601 aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca ttgtctatca
    15661 ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta caagaaatga
    15721 agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa gaagccggcg
    15781 aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa aaaatgggat
    15841 tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta ctggaacagc
    15901 ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt gcaaaaattc
    15961 aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa gtgcataccc
    16021 gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct aatttgcaga
    16081 acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt cctagagaaa
    16141 aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta cttgcgcata
    16201 tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc catgccaaca
    16261 cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat atgcgtaggc
    16321 aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga ctttcacaaa
    16381 atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt gaaaaatacc
    16441 caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa ggctacgtcg
    16501 aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat tacaatatcc
    16561 gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct gctgatattt
    16621 tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt caagctacga
    16681 tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa cttgaatcat
    16741 tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta ccactgatca
    16801 ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt aagacagagc
    16861 aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa ccgttcgtaa
    16921 aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct tgtggcccaa
    16981 gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg aaaccatcca
    17041 atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg aactgatctc
    17101 acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa tcgataaaca
    17161 tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg atgtacgaaa
    17221 atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag gaatcatgaa
    17281 attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa atggactgaa
    17341 aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga ctggtctagg
    17401 taatatttat gtagatgaag cattatggga agcgaagatc catccagaac aaccggcgaa
    17461 cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg ttttggatcg
    17521 tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag gagaatcagg
    17581 cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta tacgttgtgg
    17641 aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc cgaattgtca
    17701 aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat agctaccggt
    17761 aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga tggcgatgtg
    17821 attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt atcagtgttt
    17881 ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg gatgattgtt
    17941 tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt tatccgaaaa
    18001 gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat tgtagatatc
    18061 ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt cgtttatact
    18121 acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct agaacaagcg
    18181 cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc ggatattttg
    18241 ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg gctttttgct
    18301 aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag aaagtgaggg
    18361 atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc aaaagaaaaa
    18421 atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca atcagaaaca
    18481 gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga ccagcaggca
    18541 gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac acctgatcgt
    18601 agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat cgaggaacag
    18661 cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa atcgagatgt
    18721 gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta gatgactaaa
    18781 tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga aaattgaaat
    18841 ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc ttatatccgt
    18901 gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac aactagttgt
    18961 aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa atgagatgtc
    19021 ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat gatggacgtg
    19081 cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca tttgaacgaa
    19141 ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag ttcaatcgcg
    19201 ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct atggaacaaa
    19261 tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa aacgaagtat
    19321 cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac gaaatcgctt
    19381 atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc ttaaaagagt
    19441 tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa gaaaacaatt
    19501 aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca accaaaaaat
    19561 atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc cttgctgtat
    19621 ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct gttatcagaa
    19681 atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct tagtagtcta
    19741 ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat cggcttactg
    19801 gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga gctgttagaa
    19861 ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct tctggaaaaa
    19921 gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag gcatacagca
    19981 gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa tgaagcagtg
    20041 tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga atctgaatca
    20101 gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga ttggctggca
    20161 aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat gtatcatcag
    20221 ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta tgactttagt
    20281 gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca gagacgtccg
    20341 gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc ctcgtcaaac
    20401 gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat tccaccgatg
    20461 aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca agaaaactgg
    20521 ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa tgttttgctg
    20581 aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt aaatacagtg
    20641 gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca gcacattcgg
    20701 caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta tcaaactggc
    20761 aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa agatgaaaaa
    20821 cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata cctggcaaaa
    20881 aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc atcagcaaac
    20941 gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat gcggatgttc
    21001 aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa aaaagttacg
    21061 ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat ccaacgatga
    21121 tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat gtcacttatg
    21181 taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc cgagtaaaag
    21241 caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa cgtacagatg
    21301 gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact gctgatccaa
    21361 aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa acatatttat
    21421 taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta gtccattttc
    21481 cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca gaaaagttag
    21541 atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat gcgatgagta
    21601 gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag gaacagttac
    21661 caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg tccgtcactc
    21721 aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata cgttatttga
    21781 caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt gacaagtcca
    21841 actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga cagaaatgac
    21901 cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt ttatgagggc
    21961 tattttttct agtatatctg tagttggtct attttttctt ttagcaggct gcggtagcgc
    22021 agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa aaacaagcag
    22081 tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatca ctcaagtaga
    22141 agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag aagatccagc
    22201 gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg ctgaaaaaat
    22261 aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat tagttgagat
    22321 gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta ttgatgtttc
    22381 attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt tatagaacga
    22441 aaaggtatat tttttaagga gatatgggaa ttt
//
LOCUS       DAZKAY010000047        22459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000047 DAZKAY010000000
VERSION     DAZKAY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.047"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8WP_001614"
     CDS             6..305
                     /locus_tag="ACG8WP_001614"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9027920.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8WP_001615"
     CDS             378..1739
                     /locus_tag="ACG8WP_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9027921.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8WP_001616"
     CDS             1761..2021
                     /locus_tag="ACG8WP_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027922.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8WP_001617"
     CDS             2035..2787
                     /locus_tag="ACG8WP_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9027923.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8WP_001618"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8WP_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9027924.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8WP_001619"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8WP_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9027925.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8WP_001620"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8WP_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9027926.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8WP_001621"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8WP_001621"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9027927.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8WP_001622"
     CDS             7161..8558
                     /locus_tag="ACG8WP_001622"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9027928.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8WP_001623"
     CDS             8639..9079
                     /locus_tag="ACG8WP_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9027929.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8WP_001624"
     CDS             9227..9553
                     /locus_tag="ACG8WP_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9027930.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8WP_001625"
     CDS             9554..10303
                     /locus_tag="ACG8WP_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9027931.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8WP_001626"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8WP_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9027932.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8WP_001627"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8WP_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027933.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8WP_001628"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8WP_001628"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9027934.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8WP_001629"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8WP_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9027935.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8WP_001630"
     CDS             14770..17436
                     /locus_tag="ACG8WP_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9027936.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8WP_001631"
     CDS             17800..18552
                     /locus_tag="ACG8WP_001631"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9027937.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8WP_001632"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8WP_001632"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9027938.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8WP_001633"
     CDS             19913..22354
                     /locus_tag="ACG8WP_001633"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9027939.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7207 a         3939 c         4682 g         6631 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttggctct tcgtcaaatt
    22441 gtgttggcga agacaaatt
//
LOCUS       DAZKAY010000048        21290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000048 DAZKAY010000000
VERSION     DAZKAY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.048"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(196..501)
                     /locus_tag="ACG8WP_001634"
     CDS             complement(196..501)
                     /locus_tag="ACG8WP_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9027940.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(526..957)
                     /gene="ruvX"
                     /locus_tag="ACG8WP_001635"
     CDS             complement(526..957)
                     /gene="ruvX"
                     /locus_tag="ACG8WP_001635"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9027941.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(954..1220)
                     /locus_tag="ACG8WP_001636"
     CDS             complement(954..1220)
                     /locus_tag="ACG8WP_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9027942.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1475..2200)
                     /locus_tag="ACG8WP_001637"
     CDS             complement(1475..2200)
                     /locus_tag="ACG8WP_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9027943.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2561..2631)
                     /locus_tag="ACG8WP_001638"
     tRNA            complement(2561..2631)
                     /locus_tag="ACG8WP_001638"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Gly,seq:ccc)
     gene            2748..3500
                     /locus_tag="ACG8WP_001639"
     CDS             2748..3500
                     /locus_tag="ACG8WP_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9027944.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3516..3995
                     /locus_tag="ACG8WP_001640"
     CDS             3516..3995
                     /locus_tag="ACG8WP_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9027945.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4246..5826)
                     /locus_tag="ACG8WP_001641"
     CDS             complement(4246..5826)
                     /locus_tag="ACG8WP_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9027946.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5819..6562)
                     /locus_tag="ACG8WP_001642"
     CDS             complement(5819..6562)
                     /locus_tag="ACG8WP_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9027947.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6738..7514)
                     /locus_tag="ACG8WP_001643"
     CDS             complement(6738..7514)
                     /locus_tag="ACG8WP_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9027948.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7762..8598)
                     /locus_tag="ACG8WP_001644"
     CDS             complement(7762..8598)
                     /locus_tag="ACG8WP_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9027949.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8609..9538)
                     /locus_tag="ACG8WP_001645"
     CDS             complement(8609..9538)
                     /locus_tag="ACG8WP_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9027950.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9730..10083
                     /locus_tag="ACG8WP_001646"
     CDS             9730..10083
                     /locus_tag="ACG8WP_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9027951.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10157..10813)
                     /locus_tag="ACG8WP_001647"
     CDS             complement(10157..10813)
                     /locus_tag="ACG8WP_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9027952.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11039..12973
                     /locus_tag="ACG8WP_001648"
     CDS             11039..12973
                     /locus_tag="ACG8WP_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9027953.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13059..14282)
                     /locus_tag="ACG8WP_001649"
     CDS             complement(13059..14282)
                     /locus_tag="ACG8WP_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9027954.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14276..16147)
                     /locus_tag="ACG8WP_001650"
     CDS             complement(14276..16147)
                     /locus_tag="ACG8WP_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9027955.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(16168..17286)
                     /locus_tag="ACG8WP_001651"
     CDS             complement(16168..17286)
                     /locus_tag="ACG8WP_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9027956.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17369..18202)
                     /locus_tag="ACG8WP_001652"
     CDS             complement(17369..18202)
                     /locus_tag="ACG8WP_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027957.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18195..19112)
                     /locus_tag="ACG8WP_001653"
     CDS             complement(18195..19112)
                     /locus_tag="ACG8WP_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9027958.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19339..20463)
                     /locus_tag="ACG8WP_001654"
     CDS             complement(19339..20463)
                     /locus_tag="ACG8WP_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9027959.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20646..>21290
                     /locus_tag="ACG8WP_001655"
     CDS             20646..>21290
                     /locus_tag="ACG8WP_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9027960.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSEN"
BASE COUNT         6607 a         4207 c         3510 g         6966 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
      241 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      301 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      361 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      421 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      481 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      541 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      601 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      661 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      721 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      781 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      841 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      901 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      961 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     1021 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggacgg gtctccagaa
     1081 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     1141 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1201 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1261 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1321 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1381 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1441 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1501 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1561 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1621 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1681 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1741 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1801 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1861 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1921 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1981 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     2041 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     2101 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     2161 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2221 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2281 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2341 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2401 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2461 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2521 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2581 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2641 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2701 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2761 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2821 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2881 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2941 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     3001 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     3061 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3121 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3181 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3241 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3301 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3361 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3421 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3481 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3541 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3601 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3661 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3721 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3781 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3841 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3901 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3961 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     4021 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     4081 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4141 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4201 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4261 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4321 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4381 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4441 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4501 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4561 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4621 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4681 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4741 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4801 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4861 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4921 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4981 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     5041 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     5101 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5161 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5221 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5281 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5341 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5401 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5461 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5521 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5581 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5641 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5701 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5761 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5821 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5881 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5941 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     6001 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     6061 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6121 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6181 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6241 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6301 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6361 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6421 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6481 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6541 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6601 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6661 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6721 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6781 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6841 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6901 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6961 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     7021 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     7081 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     7141 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7201 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7261 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7321 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7381 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7441 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7501 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7561 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7621 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7681 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7741 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7801 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7861 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7921 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7981 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     8041 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     8101 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     8161 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8221 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8281 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8341 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8401 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8461 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8521 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8581 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8641 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8701 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8761 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8821 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8881 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8941 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     9001 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     9061 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     9121 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9181 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9241 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9301 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9361 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9421 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9481 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9541 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9601 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9661 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9721 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9781 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9841 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9901 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9961 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    10021 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    10081 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    10141 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10201 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10261 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10321 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10381 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10441 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10501 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10561 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10621 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10681 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10741 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10801 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10861 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10921 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10981 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    11041 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    11101 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    11161 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11221 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11281 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11341 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11401 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11461 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11521 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11581 acaccctgac attttgattc tagatgaacc aacgaaccat ttagatatcg agacactttc
    11641 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11701 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11761 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11821 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11881 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11941 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    12001 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    12061 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    12121 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12181 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12241 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12301 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12361 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12421 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12481 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12541 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12601 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12661 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12721 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12781 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12841 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12901 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12961 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    13021 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    13081 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    13141 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13201 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13261 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13321 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13381 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13441 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13501 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13561 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13621 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13681 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13741 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13801 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13861 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13921 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13981 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    14041 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    14101 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    14161 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14221 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14281 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14341 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14401 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14461 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14521 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14581 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14641 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14701 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14761 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14821 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14881 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14941 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    15001 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    15061 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    15121 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15181 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15241 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15301 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15361 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15421 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15481 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15541 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15601 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15661 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15721 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15781 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15841 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15901 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15961 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    16021 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    16081 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    16141 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16201 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16261 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16321 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16381 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16441 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16501 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16561 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16621 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16681 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16741 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16801 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16861 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16921 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16981 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    17041 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    17101 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    17161 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17221 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17281 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17341 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17401 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17461 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17521 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17581 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17641 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17701 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17761 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17821 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17881 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17941 tggctgtagc taaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    18001 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    18061 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    18121 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18181 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18241 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18301 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18361 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18421 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18481 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18541 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18601 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18661 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18721 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18781 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18841 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18901 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18961 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    19021 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    19081 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    19141 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19201 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19261 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19321 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19381 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19441 cagcataaat gtaagaaatt gttgtgccac ttgtggattt tttgcatctt tagggacata
    19501 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19561 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19621 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19681 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19741 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19801 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19861 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19921 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19981 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    20041 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    20101 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    20161 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20221 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20281 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20341 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20401 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20461 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    20521 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    20581 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    20641 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    20701 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    20761 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    20821 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    20881 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    20941 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    21001 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    21061 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    21121 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    21181 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    21241 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat
//
LOCUS       DAZKAY010000049        20578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000049 DAZKAY010000000
VERSION     DAZKAY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.049"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8WP_001656"
     CDS             352..1041
                     /locus_tag="ACG8WP_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9027961.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8WP_001657"
     CDS             1028..2236
                     /locus_tag="ACG8WP_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9027962.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8WP_001658"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8WP_001658"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9027963.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8WP_001659"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8WP_001659"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9027964.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8WP_001660"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8WP_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9027965.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8WP_001661"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8WP_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9027966.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8WP_001662"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8WP_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9027967.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8WP_001663"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8WP_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9027968.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8WP_001664"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8WP_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9027969.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8WP_001665"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8WP_001665"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9027970.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8WP_001666"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8WP_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9027971.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8WP_001667"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8WP_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9027972.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8WP_001668"
     CDS             14660..15043
                     /locus_tag="ACG8WP_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9027973.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8WP_001669"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8WP_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9027974.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8WP_001670"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8WP_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9027975.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8WP_001671"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8WP_001671"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9027976.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8WP_001672"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8WP_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9027977.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACG8WP_001673"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACG8WP_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9027978.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..>20578
                     /gene="dnaN"
                     /locus_tag="ACG8WP_001674"
     CDS             19466..>20578
                     /gene="dnaN"
                     /locus_tag="ACG8WP_001674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9027979.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLIT"
BASE COUNT         5876 a         4203 c         3787 g         6712 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattact
//
LOCUS       DAZKAY010000050        20561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000050 DAZKAY010000000
VERSION     DAZKAY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.050"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(391..2466)
                     /gene="glyS"
                     /locus_tag="ACG8WP_001675"
     CDS             complement(391..2466)
                     /gene="glyS"
                     /locus_tag="ACG8WP_001675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9027980.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2468..3385)
                     /gene="glyQ"
                     /locus_tag="ACG8WP_001676"
     CDS             complement(2468..3385)
                     /gene="glyQ"
                     /locus_tag="ACG8WP_001676"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9027981.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3769..4581)
                     /gene="recO"
                     /locus_tag="ACG8WP_001677"
     CDS             complement(3769..4581)
                     /gene="recO"
                     /locus_tag="ACG8WP_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9027982.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4724..5623)
                     /gene="era"
                     /locus_tag="ACG8WP_001678"
     CDS             complement(4724..5623)
                     /gene="era"
                     /locus_tag="ACG8WP_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9027983.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5638..6033)
                     /locus_tag="ACG8WP_001679"
     CDS             complement(5638..6033)
                     /locus_tag="ACG8WP_001679"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9027984.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6017..6493)
                     /gene="ybeY"
                     /locus_tag="ACG8WP_001680"
     CDS             complement(6017..6493)
                     /gene="ybeY"
                     /locus_tag="ACG8WP_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9027985.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6510..8702)
                     /locus_tag="ACG8WP_001681"
     CDS             complement(6510..8702)
                     /locus_tag="ACG8WP_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9027986.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8726..9697)
                     /locus_tag="ACG8WP_001682"
     CDS             complement(8726..9697)
                     /locus_tag="ACG8WP_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9027987.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10403..11782
                     /gene="brnQ"
                     /locus_tag="ACG8WP_001683"
     CDS             10403..11782
                     /gene="brnQ"
                     /locus_tag="ACG8WP_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9027988.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11938..12384)
                     /locus_tag="ACG8WP_001684"
     CDS             complement(11938..12384)
                     /locus_tag="ACG8WP_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9027989.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12411..12587)
                     /gene="rpsU"
                     /locus_tag="ACG8WP_001685"
     CDS             complement(12411..12587)
                     /gene="rpsU"
                     /locus_tag="ACG8WP_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9027990.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12749..13177)
                     /locus_tag="ACG8WP_001686"
     CDS             complement(12749..13177)
                     /locus_tag="ACG8WP_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9027991.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13269..14129
                     /locus_tag="ACG8WP_001687"
     CDS             13269..14129
                     /locus_tag="ACG8WP_001687"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9027992.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14213..14563)
                     /locus_tag="ACG8WP_001688"
     CDS             complement(14213..14563)
                     /locus_tag="ACG8WP_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9027993.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14579..16306)
                     /locus_tag="ACG8WP_001689"
     CDS             complement(14579..16306)
                     /locus_tag="ACG8WP_001689"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9027994.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16519..17445)
                     /gene="trxB"
                     /locus_tag="ACG8WP_001690"
     CDS             complement(16519..17445)
                     /gene="trxB"
                     /locus_tag="ACG8WP_001690"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9027995.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17590..19023)
                     /locus_tag="ACG8WP_001691"
     CDS             complement(17590..19023)
                     /locus_tag="ACG8WP_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9027996.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19023..20426)
                     /locus_tag="ACG8WP_001692"
     CDS             complement(19023..20426)
                     /locus_tag="ACG8WP_001692"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9027997.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5592 a         4450 c         3652 g         6867 t
ORIGIN      
        1 attgacgtca taaaaagtag ctttgaattc ataatcgttt atataacgat taatagaaaa
       61 caatatagta tttagtgttc atacaatgat tctaagatca ttttgaatta atcagtctta
      121 tagttgattt ctaaattgtc atatattatt aaaaattttt gccatttaaa aagaaaagcg
      181 acagtttacc aataaatttc aactgaatta tctaaaagta gatcagttag tttagataat
      241 tcagttgaaa ttgaccgact caaattactt gtataatacc taggaaaaaa ttaactagca
      301 ccacgggtat agttatttat atttataaca aataaaccag agaaatagcc tattttgtgc
      361 ctattctctg gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc
      421 tagcgacaaa gccatggcag caatttgact taattgcgtt aatcggttct tacgtactgc
      481 tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct
      541 tacaagctga tcataatttt cgttgatcgt ttggtcagca aagctttttt tcattgcctc
      601 tactgctttg tgcagttctt tctccgcttc attttcgaac agttcagggt ctacttctga
      661 ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga
      721 tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt
      781 tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac gaatattctt
      841 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt gttgcccttc
      901 tgataaacca atgccgaaac gatccgtatc tttactgata acatcactaa tcgtttcttg
      961 cagaacagac aaaggaaagg cccatttttt cgattcgatg atccgaacga caccgtatgc
     1021 ttggcgacgt aaggcataag gatcgtttga cccgcttgga atcatcccaa ctgcaaagaa
     1081 agaaaaaatg ctatctaatt tatctgcaat cgctaaaaca gaacctactg tagactgagg
     1141 tagctctcct tcactggata taggcatgta atgctcacga atcgctgttg ctacagcagg
     1201 tttctcgcct ttcagcagag catatttttc gcccatgatc ccttgtagtt caggaaattc
     1261 ccctaccatg ttggttacta gatcaaattt atagatttca gcagctcgtt gcagatccat
     1321 caattcttcc tcagatagtc ctacttgttc gccaatgata cgggcaatga cttgtacccg
     1381 ctgcattttc tcatagatac taccgatttt ttcatggaaa gtaacagttt ttagtttttc
     1441 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1501 acgagccgtc aatacttttt gattgcctcg aaccacattt tccaatttta cccgatcacc
     1561 attacgagca gaaataaaat gaggaagcag cataccttga tcgttccgta cctcgaaata
     1621 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa gatatttttc
     1681 ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa
     1741 atcttcgtct agatcaataa cccagtgatt tttttcggct aattcatttg cttgtgtaac
     1801 gatcatttct tgtctttttt gcggtgaagc aataacgaat tgttcattca atttttcttc
     1861 atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgtc ctcgagaggc
     1921 tctgcctgtt ttgacatcta agatctcaaa cgggattact tcgtcatcta acaatgccac
     1981 gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca tcgttactgg
     2041 gaaagtgagg cttttaatga catctgctaa acctgtcaat acttctttcg cttctttgcc
     2101 gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc
     2161 atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc
     2221 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt ctgcttgacg
     2281 ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt
     2341 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat
     2401 gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt
     2461 tgccatctta ttcagcctcc ttcatgtttt ctggatttgc tgtgttttta ttcaataaag
     2521 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2581 ggatacggga aagataacct gctcgttctg ttacggatac tgcaccgcga gcatctaata
     2641 aattgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca agattttcat
     2701 cgatacagcg tttagcttct ttttcaaaac ggtcaaagtt gcttagtagc aagtcttggt
     2761 cacttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag atctcgccat
     2821 atttcacacc atcagcccat tccagatcat agacactttc aacttcttga atatatgaag
     2881 caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga
     2941 cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc aagccatact tcccaaccca
     3001 caccggcaca ccccatcgat gggttctccc agttgtcttc aacaaaacga atatcgtgtt
     3061 ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag
     3121 gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc
     3181 cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg
     3241 gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat
     3301 aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3361 cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt
     3421 caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg
     3481 acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3541 tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct
     3601 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttcttgt
     3661 ttatataata gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa
     3721 gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt
     3781 ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga
     3841 cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa
     3901 ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat
     3961 cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg
     4021 ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccag atttggaaga
     4081 atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat
     4141 tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg atgatttctg catcatctcc
     4201 ttgatccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg
     4261 atcttcgatt gctgcatcta ctagatttag caaataggta ccataaccgc tcaaaaagat
     4321 atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc
     4381 ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa
     4441 tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt
     4501 aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt
     4561 cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc
     4621 tgccgatcca gattatttat gtttcgatct tccgccgttt ttctcaacgt gcggattttt
     4681 atccagttta tttctatact taacctataa gaagttcaga atatcagtat tcgtcttttc
     4741 ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc
     4801 aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc
     4861 ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt
     4921 cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa
     4981 caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga
     5041 tcaattcaga aacgataaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt
     5101 gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt
     5161 ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga
     5221 ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga taaactggag
     5281 aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccgcgt ttttgatcag
     5341 cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca
     5401 taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct
     5461 gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac
     5521 tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg
     5581 ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta
     5641 attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa
     5701 acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg
     5761 aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac
     5821 tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa
     5881 aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc
     5941 ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt
     6001 gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac
     6061 caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc
     6121 catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt
     6181 cagcagcacg ttcaatagaa atcatgatgt ctcctagtac acgaggcaaa ggattctctc
     6241 catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga
     6301 cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt
     6361 ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt
     6421 gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa
     6481 aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca
     6541 acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg
     6601 atcaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga
     6661 ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc
     6721 ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct
     6781 tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg
     6841 cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa
     6901 atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca
     6961 tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct
     7021 cctgaaggca agttctcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa
     7081 tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt
     7141 agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc
     7201 aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc
     7261 atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg
     7321 aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg
     7381 atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct
     7441 tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac
     7501 atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcagat
     7561 actgcttgga aaataccagc catgatccct gctcttcggt tcaagaaaaa gcttaaaaca
     7621 agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat
     7681 aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg
     7741 tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt
     7801 tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa tcgaagtcgt ttgattcgtc
     7861 aatcctagta attccaattt tttcatcgct gaagcatcaa tttgatttcc ttcgcgaaca
     7921 atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcaga ttgttttaat
     7981 tcttctgtct ttttctcatt caatacatca ttgactacaa tgccttgatc cactaagtag
     8041 cgaatcattt gttgctgctc tgttgtgaca ttcaaatact gaatctgatc ttctgcttct
     8101 tgtttaaaag cttccaattc gctttcacga acttgtttac tcatctgttc gtcaatgagc
     8161 ttcaaacttt catccctaac cgtgtgaatt tccgtttcag tcatttcaaa tatcgtcgaa
     8221 taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt ctgcattcac gttttcaaaa
     8281 tttttcttca gtgtagcaat ccgctcttct gtagtaggtt tcgggatatt ttcatcttcc
     8341 ttcgccttat ctaccttgtc attataagat ttgttgatgg tatcattcgt cttatcgatc
     8401 aattcgaata attgcttgat ccgattgttc tggtcatctg ccagatcttt ttgataagta
     8461 tattctggag tcactgcttc tgctgccagt tttcttcttt gttcggtttc ttccgtgttc
     8521 tcgatcgttt tattcgcacg gatactctct tctgctactt ggccttctct gaaatcattg
     8581 gccttttgtt tgacactact aaatgtaacg ccgcagacaa tgataaaaaa caagaacaga
     8641 aggaaaggca atgctgcctt tccgagtttt tcttgtagtt tctgcagtgt tttattcaac
     8701 atgtgcttat cctcctaagc aaagcttatt cttgatgaag atccgccttt tcataagctt
     8761 tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat
     8821 cgattttttt gatttgcttt agtgttcgtt ctgcatgaac aagaccactt gtcgtccctc
     8881 gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac
     8941 gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga
     9001 atgcatcatc caatgtccgc ccccgcatgt aagctaatgg tgcgatttcg attacgcctc
     9061 gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag
     9121 gccgcagata aggatccact ttttctttca gatccccggg tagaaagcct aggttttccc
     9181 ctgcctctac agcgggccgc gtcaaaatga ttttttgcac ttcgcctttt tttagggcag
     9241 caattgctaa tactacggca agaaacgttt tccctgttcc ggctggccct acaccaaata
     9301 cgatgtcatg tttggctacc gattcaacgt atttcttttg accgctgttt tttacccgta
     9361 taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta
     9421 atgtaccttt ttgccccatt ttcaatgccg tcacgacatc tggtgtactt acgtggattc
     9481 ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt
     9541 gttcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct
     9601 cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagctg
     9661 tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg
     9721 attcattctt tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt
     9781 gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa aaaatagctg tttccattcg
     9841 ctaaaggtct cgttttgata aaaagaatct gagaatgtaa gttatatgaa agtggagaac
     9901 aaaatattcc gtttcattca gaggtattct ctaggtattt ttcttgtttc aaaaaaagaa
     9961 aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca
    10021 aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt
    10081 gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat
    10141 gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata actttatcaa
    10201 aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc
    10261 tagagccctt ttcccaagtg gaaaagggct tttttttata ttttaaggtg gtggttttca
    10321 tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc aatcaaaatg
    10381 ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga aacaactatt
    10441 gatggtcagt tcacttatct tcgggatgtt tttcgggtca ggtaatctaa tctttcctgt
    10501 ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat ttgccatctc
    10561 aggcgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa gtgatggtct
    10621 gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa tccttgttca
    10681 tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt ttgaaatggc
    10741 tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat tctctgctct
    10801 tttcttttta cttgcttatt tccttactgt ccatcccaca cggttggtca aatacgtcgg
    10861 aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg tttctttatt
    10921 ccacccaatg ggaaatctaa atcaatctgc tgcagatact taccaaacaa acgctttaac
    10981 aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gccttgcttg ccttaagtgt
    11041 cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca cagaattaac
    11101 agcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg gtcttgttct
    11161 tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc
    11221 tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag tgatggtcac
    11281 attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcacaag atttccataa
    11341 actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg tctcttttgt
    11401 cgttgcaaat gctggtttgg acaatatcat ccaatggtct ttaccagtat tgatgctttt
    11461 gtatccattg tcgcttgcgt tgattttgct ttccttaaca gcaaaatttt tccaaaagac
    11521 accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cccgccgtat tggatatgct
    11581 ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc tcgaatttta
    11641 tcatcatcac gttccttttg ctgcattggg acttggctgg atggtcccaa cgttacttgg
    11701 gtatgctggc agcttactct tctattacgc ctatcgccta tcagggtata aacaagaagc
    11761 aaatgaacta ccagaagaat aaccagatca aacacaattt aacataagaa atattaaaca
    11821 ggaaaaaaac agctagatga aagaatccta ttctttcgtc tagctatttt ttagatctct
    11881 tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat gataaaatta
    11941 agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt ttcctttgac
    12001 tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag caccagtttg
    12061 cgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt ccggcatgta
    12121 tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac gtcctgcttc
    12181 ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca ccgttaattc
    12241 ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag atgacttgat
    12301 cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt tcatgtcatc
    12361 gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa ctagaatttt
    12421 ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt ttcgtagaat
    12481 tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt gaagcgacga
    12541 agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt aattccctcc
    12601 ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa acaagactgt
    12661 cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct tttttttaaa
    12721 attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt ccgaaaatct
    12781 cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa ttcattggac
    12841 acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg tgatggccga
    12901 tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg gtttcttcca
    12961 gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa cttactcctt
    13021 tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga ttgcgcttca
    13081 ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta tttttcaatg
    13141 tttctagtga ttgttcgatc aacgtctgtt ttttcatgac ttattcctcc aaatcaaaat
    13201 gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat gcaagtgagg
    13261 ttttatccat gtcttccgat tctacagaaa tcgttacttt ttttgtcatc tccgactctg
    13321 ctggagaaac tgcgacaaaa ctagcgcaag ccactatggc ccaatatcct agcgttgagt
    13381 tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag gcactgcaag
    13441 atgcactaga agaaaaagct cttgtcctgc acacattgat caatcaagaa ttgatcgaga
    13501 tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg actccgccga
    13561 tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc gcccttcatt
    13621 tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta aaatacgatg
    13681 acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc gtatcccgaa
    13741 cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaagtt gccaatctac
    13801 ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct aaaaaaatcg
    13861 tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg atgcgctctt
    13921 acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct gaactcgatt
    13981 ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct tctttatcta
    14041 ttgaagaaac agcttcgctt attttgaatg ctttagacct agaagaccat agttattatg
    14101 gaactgaaac caccgaagaa acagactaac aatttttctt taaaaaaact ggactgtgtc
    14161 agcgaagaag attcgttgac acagtccagt ttgtctctat ttgcacctat cgttattgat
    14221 gttccacatg tttgagtgtc tggttcaaaa tatccagtac gtgagaatca tcaagactat
    14281 acaaaatcgc ttttccctca cgatgggact tgacgacatg gttttctctt aataaacgca
    14341 gttgatgcga tacagcagat tgttccattt ccactgcttg gatgattgct gtcacatttt
    14401 gttccccgtc tttcagatac agcagaattt tgagacgagt ggggtcactc aacattttga
    14461 atagttggct gactttctct acttttgttt tttgcgtgtc tggcaatcca ttcacctcca
    14521 acttgaaaaa agactgactt ctaaagttta ccactttttc catcagtctc aagttttttt
    14581 attcttctgt catcttttgg ataatcgttt cataagcggc tactttttca gctgcgtctt
    14641 ctgaagagtt tccttttaca ccgatataaa atttgatttt cggctcagtt cctgaaggac
    14701 ggatagcgat ccagccattt tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg
    14761 ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt
    14821 ggatgacttt tgctccggca aattctgttg gtgcttcttc acggaatttt tccatcaaag
    14881 cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt
    14941 agtagccata ggtttcaaaa atcgtttgca agccatcata caacgttttg ccttgttttt
    15001 tatagtaagc tgctacttct gctaataaga gcaatgcttg gatcgcgtct ttgtcgcgaa
    15061 cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat
    15121 gatcttcttc aaattgttga attttttcag caatgaattt gaatcctgtc aatacattga
    15181 acatggtagc cccatagtgt tccgcaattg ctgtaggtaa ttcgctagaa acgatcgatt
    15241 tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt
    15301 gagcaaacag tgctccgatt tggtttccgg tcaatacttc ataggagcca tttggcaaac
    15361 gaacggctgc acctaaacgg tctgcatcag gatctgttgc aatcagcaaa tcagcgcctt
    15421 ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg ttcttctgga tttggtgatt
    15481 tgactgtagt gaaatctggg tctgcgacag cttgttctgg aactagtacg aattgttcga
    15541 atccagcttg tttcaacgca cgttcgccca acatttttcc tgttccgtga agtggtgtgt
    15601 acacaagttt caaatctttc cccattgttt ccaccaattc atgatcaatc gttactgctt
    15661 tgatttcttc tagataagcg gcatccactt cttctccgat gatcgtgatc aatccgctat
    15721 gttcagcttc ttcgtcagat aatacttcaa cttttaacgg attgctgact tcacggacat
    15781 atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc cccatcttct ccgtagactt
    15841 tatatccgtt gtaagctgct ggattgtggg atgcggttac catgattccc gcaaacgtct
    15901 tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg tagactttcg aatacataag
    15961 atgggatatt gtgatgtgct aatgttttgg ctgcttccat ggcaaattct ggtgattgat
    16021 ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcgcct tgtgtatcca
    16081 tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata gatgttcatt cgattgatcc
    16141 caggccctaa gattccccgc atgcctgcag taccgaattc caacggtgca taaaaagcat
    16201 cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag ttttcttcta
    16261 aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa ataacacctc
    16321 tttctgaaat atggttctta ataagtatag cattcactta ttagatatga aagtatgagg
    16381 accaaaaaac gcttaaatca agaaaggatt cttttatcaa gcacggttgc aagtattcgc
    16441 ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt aaaagaacaa ttaaaccgtt
    16501 cttccactct ttttaaactt atgcagttgt ttcttctaaa gattccaagt atttatacac
    16561 ttgttgtcca gcgatcccac catcaccaac agcagtagtg atttgtcgca aatctttttc
    16621 acgaacatct ccgatggcaa aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg
    16681 aatccagcca gcttcgttcg tcaaccctgc ttctctaaat ggtgcagtta atggatccag
    16741 tccaacatag atgaatactc cgtctgctgc caaatcaaac tcattgccgg ttttgacatt
    16801 acgtgctttc acacctgtca ctaccatttc attcccaacg atttcgtcaa cgactgtatc
    16861 ccacataaag gaaatttttt cattcgcaaa tgcacggtct tggatgatct tttgtgcacg
    16921 taattcatct ctacggtgga cgatgactac ttcagaagca aattgtgtca aatagatggc
    16981 ttcttctact gctgagtctc ctcctccgat cactactaga cgtttgttac ggaaaaacgc
    17041 accatcacaa actgcacagt aagaaacgcc acgtccagca aattcttcct cgccaggtac
    17101 gcctaattta cggtggatac agcctgttgc gataatgact gtttttcctt cgtaagtttt
    17161 atcttcacaa ataacttctt tgtggctgcc gtgatccttg atatcttgca cgattccata
    17221 agcattttct gtcccaaatc ttgaaacacc ttcatacatc ttataagcta gatcaggacc
    17281 cattattgaa tcgaaaccag gatagttttc gatttctgcc gtattgttca tttggccgcc
    17341 aggggcgcct tgttcgatca tcaatacgga taagtttgaa cgtgaagcat ataaagcagc
    17401 agtcatccca gcaggacctg cgccaataac gattacatca tacatatgta gattctcctt
    17461 tacttaatca agtaagcata ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg
    17521 cgtttctcga atagctttat tatactcgaa aaatccgctg aaaaacaata gacttttata
    17581 aatcattgat caatcattcg aaagattttc tccatttgaa tcaattactt ttttatacca
    17641 gttgaatgat ttcttcttcg aacgggctaa tgtgccattt ccttcgttgt cacgatcgac
    17701 ataaatgaat ccgtaccttt tcttcatctc acctgtacca gctgatacta aatcaataca
    17761 tccccaagtc gtatagccta atagatcgac tccatctaat tctactgcat ctttcattgc
    17821 ctggatatgt gctgccagat agtcgattcg atagtcatct tccacataac cattctcatc
    17881 gggcgtatcg actgcaccta agccgttttc tacaataaat agtggttttt gataacgatc
    17941 ataaatatca ttcatcgtta ttctcaagcc taatggatca atttgccaac cccattcact
    18001 tgcttccaga tatggatttt tgacagaagc aaagatattt cctgcagtct gttccaagag
    18061 ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa aaggagataa aatcaacagt
    18121 atgttctttc agcaatgcgg catccccagg ttccattttg atctgaatgc catttcgttc
    18181 catttctttc aatgcataag caggatattc tcctcgtgac tggacatcaa tgaagaaata
    18241 attttctcga tctgattttc tagaagccca aacatcttca ggattgcaag tgtatggata
    18301 atatgctcct gctgccagca tacagcctac tttgttctcc ggatctactt catgagcaat
    18361 cttcgttgcg atggcacttg caaccaactc atgatgtgcc gcttgatatt tgatttgttc
    18421 ttcattttct ccttcttcaa aatacagccc tgcgcccatg aatggtgcat gaaggatcat
    18481 attgatctca ttgaatgtca accaatattt gacaagtcct ttatagcgat tgaagatgac
    18541 tcggcacaga ttttcataaa aaccaacgag ttctctggaa cgccacgcac catattcttt
    18601 gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca agcggctcga tcccgtactt
    18661 ttgacattct ttaaagacat cctcataaaa tttcaaacca gattcatttg gctctttttc
    18721 atcgcccatt ggaaaaatac gactccaagc aatggacatc cgataagtct tgaaccccat
    18781 ttctgcaaat aaagcaatat cttccttata atgatgatac atatcgatcg caatttttgc
    18841 tggataaaaa tgattttcat caaattcaaa cattttcttt tttccagtaa tgacaggaaa
    18901 acggtcttct ccgattggca caacatctac gttcgctaac ccacgtccgc cttcatcata
    18961 accgccctcg cattgattgg ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc
    19021 catcatactt cctccattac atgtgtattg cttgataatt ctcttgccag ttcatcttgg
    19081 acttcgattc cttctgcttt taaccggctg attgtttcct tgaattgcgg taaatactgc
    19141 tgatgtgcaa taaacaactc attcatgatc cgttttgctg tctttcctga tcgtataagc
    19201 ggattgatcg taaacgcttg taaagctgtt ccataatcac cagtcacagc tgcttcgatc
    19261 gtcagcaatt ccatattctt cattacttgc aaccagcctc tttcagcagc aggaagggaa
    19321 ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg cagtgacttc tactacacaa
    19381 tctgccggca gatcaggaac agctccatca ttctttgtag ataccacgat ctgtgtttct
    19441 ttatttccat agatcgaagc aatcgtttca caggcagcat ctgaataata agcaccacct
    19501 cgtttttgca attgttcagg tttatgatcc aattctggat ctttgtataa ttcaaacagc
    19561 tctgcttctg tttctttcac ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa
    19621 gcatgttcca gcatttcttc ttcacggtag taatagcggt ggtagccaca agggatcatg
    19681 ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa agatattttt cggcaatcca
    19741 ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc tttattatct
    19801 gctactttgt gccaatggaa gtgatttaga ccggcaaact tgtagatcag ctcttctttt
    19861 ttcttgccaa gcattgttgg ttctgtcatc atcgccatta ctggcacatt gcataatcct
    19921 atcactttat cccatttgcc atatctgata actgcttctg tcaccatccc gcttgggttc
    19981 gcgaaattga tcagccaagc atttggacat aaacgcttca tgtcttcaac gattgccaaa
    20041 accactggaa cggtacgaaa agctttgaat atcccgccag caccatttgt ttcttgccca
    20101 atcatcccat aataactagg gatccgctcg tctttgattc gtgcatctaa ttgaccgaca
    20161 cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc cagagttgca
    20221 tagattttga cttcatacgg agaagcatcc cacatacgct gagacatctg actgacgatc
    20281 tcaagctttt cttttccttc ttctacatct acgagccaga tttctcgtat cggtaattcc
    20341 tcatatcttt tgataaatcc ttccatcaac tcgggggtgt aactgctgcc tccaccaatc
    20401 gtagcgattt tgattccttt tgccatgttc tcactccttt actttttaca cttttattat
    20461 cttttttgta aacaaaacaa acaatagaaa ttcgtttctt tccagtaaca tttacaaaag
    20521 ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa a
//
LOCUS       DAZKAY010000051        20268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000051 DAZKAY010000000
VERSION     DAZKAY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.051"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACG8WP_001693"
     CDS             complement(89..1648)
                     /locus_tag="ACG8WP_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9027998.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8WP_001694"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8WP_001694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9027999.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8WP_001695"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8WP_001695"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9028000.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8WP_001696"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8WP_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9028001.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8WP_001697"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8WP_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9028002.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8WP_001698"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8WP_001698"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9028003.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACG8WP_001699"
     CDS             9410..10465
                     /locus_tag="ACG8WP_001699"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9028004.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8WP_001700"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8WP_001700"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9028005.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACG8WP_001701"
     CDS             11952..12080
                     /locus_tag="ACG8WP_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9028006.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACG8WP_001702"
     CDS             12520..12708
                     /locus_tag="ACG8WP_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9028007.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACG8WP_001703"
     CDS             12786..13799
                     /locus_tag="ACG8WP_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9028008.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACG8WP_001704"
     CDS             complement(13866..14900)
                     /locus_tag="ACG8WP_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9028009.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="ACG8WP_001705"
     CDS             15015..17240
                     /locus_tag="ACG8WP_001705"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9028010.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="ACG8WP_001706"
     CDS             17258..19141
                     /locus_tag="ACG8WP_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9028011.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPVKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="ACG8WP_001707"
     CDS             19122..20096
                     /locus_tag="ACG8WP_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9028012.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6825 a         3720 c         4342 g         5381 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtccacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgtga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa gctgctttat tctatatgtc
    20161 atgaacaaga gagtaaaaaa gttctcagat acttttgttc atgactattt tcttcaataa
    20221 aaccaattgt tatttttctg aaaaacaaag ttaactatca gaaaatta
//
LOCUS       DAZKAY010000052        20186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000052 DAZKAY010000000
VERSION     DAZKAY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.052"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..1018
                     /gene="proB"
                     /locus_tag="ACG8WP_001708"
     CDS             212..1018
                     /gene="proB"
                     /locus_tag="ACG8WP_001708"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9028013.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1015..2262
                     /locus_tag="ACG8WP_001709"
     CDS             1015..2262
                     /locus_tag="ACG8WP_001709"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9028014.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2338..2682
                     /locus_tag="ACG8WP_001710"
     CDS             2338..2682
                     /locus_tag="ACG8WP_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028015.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2799..3125)
                     /locus_tag="ACG8WP_001711"
     CDS             complement(2799..3125)
                     /locus_tag="ACG8WP_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028016.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3498..4700)
                     /locus_tag="ACG8WP_001712"
     CDS             complement(3498..4700)
                     /locus_tag="ACG8WP_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9028017.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4817..5332
                     /locus_tag="ACG8WP_001713"
     CDS             4817..5332
                     /locus_tag="ACG8WP_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9028018.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5531..5977)
                     /locus_tag="ACG8WP_001714"
     CDS             complement(5531..5977)
                     /locus_tag="ACG8WP_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9028019.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6024..7502)
                     /gene="msr(C)"
                     /locus_tag="ACG8WP_001715"
     CDS             complement(6024..7502)
                     /gene="msr(C)"
                     /locus_tag="ACG8WP_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9028020.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7856..8485)
                     /locus_tag="ACG8WP_001716"
     CDS             complement(7856..8485)
                     /locus_tag="ACG8WP_001716"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9028021.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8469..8933)
                     /locus_tag="ACG8WP_001717"
     CDS             complement(8469..8933)
                     /locus_tag="ACG8WP_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9028022.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9420..10688)
                     /locus_tag="ACG8WP_001718"
     CDS             complement(9420..10688)
                     /locus_tag="ACG8WP_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9028023.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10919..11656)
                     /locus_tag="ACG8WP_001719"
     CDS             complement(10919..11656)
                     /locus_tag="ACG8WP_001719"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9028024.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11723..12292)
                     /locus_tag="ACG8WP_001720"
     CDS             complement(11723..12292)
                     /locus_tag="ACG8WP_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9028025.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12420..13292
                     /locus_tag="ACG8WP_001721"
     CDS             12420..13292
                     /locus_tag="ACG8WP_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9028026.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13634..14506
                     /locus_tag="ACG8WP_001722"
     CDS             13634..14506
                     /locus_tag="ACG8WP_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9028027.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14503..16461
                     /gene="nagE"
                     /locus_tag="ACG8WP_001723"
     CDS             14503..16461
                     /gene="nagE"
                     /locus_tag="ACG8WP_001723"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9028028.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16712..17563)
                     /locus_tag="ACG8WP_001724"
     CDS             complement(16712..17563)
                     /locus_tag="ACG8WP_001724"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9028029.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17686..18153
                     /locus_tag="ACG8WP_001725"
     CDS             17686..18153
                     /locus_tag="ACG8WP_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9028030.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18416..18898)
                     /locus_tag="ACG8WP_001726"
     CDS             complement(18416..18898)
                     /locus_tag="ACG8WP_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9028031.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18998..19861)
                     /locus_tag="ACG8WP_001727"
     CDS             complement(18998..19861)
                     /locus_tag="ACG8WP_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9028032.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6070 a         4158 c         3663 g         6295 t
ORIGIN      
        1 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
       61 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actacaagtt
      121 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
      181 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
      241 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
      301 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
      361 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
      421 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
      481 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
      541 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
      601 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
      661 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
      721 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
      781 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
      841 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
      901 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
      961 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
     1021 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
     1081 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
     1141 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
     1201 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
     1261 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
     1321 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgaccggcat catttacgaa
     1381 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
     1441 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
     1501 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
     1561 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
     1621 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
     1681 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
     1741 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
     1801 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
     1861 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
     1921 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
     1981 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
     2041 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
     2101 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
     2161 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
     2221 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
     2281 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
     2341 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
     2401 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
     2461 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
     2521 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
     2581 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
     2641 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
     2701 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
     2761 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
     2821 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
     2881 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
     2941 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
     3001 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
     3061 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
     3121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
     3181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
     3241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
     3301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
     3361 caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3421 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3481 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3541 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3601 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3661 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3721 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     3781 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     3841 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     3901 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     3961 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4021 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4081 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4141 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4201 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4261 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4321 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4381 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4441 ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac
     4501 atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc
     4561 gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct
     4621 acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt
     4681 tgattctcca atatttccat ccctttctct ctattagcat catacatgat ttattttaca
     4741 tgtaaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac
     4801 gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa
     4861 accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag
     4921 tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa
     4981 agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga
     5041 ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat
     5101 ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca
     5161 acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa
     5221 gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga
     5281 actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa
     5341 cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca
     5401 gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt
     5461 ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa
     5521 cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa
     5581 cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag
     5641 cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg
     5701 tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg
     5761 gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca
     5821 aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat
     5881 cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg
     5941 ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg
     6001 tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc
     6061 ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta
     6121 aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata
     6181 tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa
     6241 gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt
     6301 tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt
     6361 tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga
     6421 tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt
     6481 tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa
     6541 gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt
     6601 tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt
     6661 tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt
     6721 ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga
     6781 tccggtctga tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt
     6841 tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct
     6901 tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc
     6961 gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga
     7021 aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc
     7081 agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt
     7141 tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc
     7201 cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc
     7261 tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt
     7321 ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca
     7381 ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat
     7441 agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc
     7501 atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga
     7561 aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg
     7621 actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac
     7681 atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc
     7741 aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg
     7801 ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt
     7861 cgaacgtagt gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt
     7921 ttcctattga taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg
     7981 ccaactgatc tgcttcgact ccaccaattg gatttgtttc atagccatga tcacgcgcaa
     8041 ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt
     8101 ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt
     8161 ttggcatctt ccctgattcg taagcttggt tatagatttc ttcgccatat tcgtaacatt
     8221 gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac
     8281 ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg
     8341 gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt
     8401 cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg
     8461 ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg
     8521 caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg
     8581 aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg ctcgattttc
     8641 ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga
     8701 cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca
     8761 atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc
     8821 atgatctcgt atctagtaag acttataccg atattttctt caaagatctg gacgattttt
     8881 tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc
     8941 tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa
     9001 aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa
     9061 agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag
     9121 attattgatt cttatcaaac tatagaagat agctcttctg cagtagaaaa ttttacaccg
     9181 ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag
     9241 tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa
     9301 acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat
     9361 ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt
     9421 taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt
     9481 tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga
     9541 taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt
     9601 gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg
     9661 tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc
     9721 cagcctacag ttggcagata atttttttct ccggcattca atgtatataa atgttctcct
     9781 gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt
     9841 gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca
     9901 taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca
     9961 ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct
    10021 tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc
    10081 ccattcacat attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc
    10141 ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg
    10201 atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca
    10261 gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa
    10321 gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt
    10381 tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct
    10441 gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt
    10501 gaaattgtat tttctgagac agtagctgac tgcaaagtac tctctgtatc gttttcaatt
    10561 cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc
    10621 actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt
    10681 gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa
    10741 aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt gataaatttg
    10801 ctttttctac ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg
    10861 atggtcgttt gaataactat ttaacaagta attctctttg gatattatcc gttatggatt
    10921 atacttcctg tgtcgtcgga cggataatca tgttactgac agacatccgg tctggcgttt
    10981 ctatcgcaaa tgctacagca gaagctatat cttcggaggt caatccccat tctttttgcg
    11041 cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa
    11101 cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc
    11161 cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa
    11221 caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga
    11281 ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt
    11341 gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt
    11401 acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg
    11461 caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta
    11521 ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta
    11581 atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt
    11641 ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact
    11701 atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag
    11761 ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa
    11821 caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag
    11881 catttttctt gatgtggatt ggctttacta acaagcctct tcgttctttt ggatcggtta
    11941 gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga
    12001 tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa
    12061 tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt
    12121 cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca
    12181 gcgctgctaa aggctcttcg ttttcagctt ggatctgatg gatcacttca aagagcggat
    12241 ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc
    12301 ctccttttat ccttacttta taacggacgg cttatcatgt ctaatactta taaaacatac
    12361 ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa
    12421 tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag
    12481 cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag
    12541 aagaacttgg tgtgacactt ttcactcggg gctcccgaga gatacagttg acggattctg
    12601 gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca
    12661 atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag
    12721 caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt
    12781 ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg
    12841 attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag
    12901 cccaagatcg ttggggactt ttgatacatc gaaatcatcc tttagcaaag caaaccgtca
    12961 tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca
    13021 gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata
    13081 acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag
    13141 atgggatcat caatacttat gggacggaaa aaatattcat cccactcgat ccgccattac
    13201 aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat
    13261 ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct
    13321 ttttaacaac gaataagata agcaaaagat ccaacgatta aataacataa gtttcctaat
    13381 ttatttcttc cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat
    13441 tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga
    13501 ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg
    13561 tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa
    13621 aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga
    13681 cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga
    13741 tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa
    13801 gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat
    13861 tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac
    13921 cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact
    13981 tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga
    14041 gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat
    14101 ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac
    14161 gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga
    14221 accattttca ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac
    14281 ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata
    14341 cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct
    14401 cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc
    14461 aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa
    14521 cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta
    14581 gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct
    14641 ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg
    14701 atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca
    14761 gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt
    14821 gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg
    14881 gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc
    14941 ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta
    15001 ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt
    15061 ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga
    15121 ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt
    15181 aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc
    15241 ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca
    15301 gaaaacaaag caaaaacagc ttccttgatg ctagcagcag cttttgcttc attctttact
    15361 ggtgtgacag aaccacttga attttcattt atgttcgttg cttggccatt atatgtcttg
    15421 catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga
    15481 tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat
    15541 cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt
    15601 aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca
    15661 gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc
    15721 tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt
    15781 cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta
    15841 cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa
    15901 ttcgttgcag atgagatggc aaaactacat ggtggtgctg cagcacgacc tgcacaaaca
    15961 aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga tcgaacaaga tatttacgct
    16021 atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa
    16081 atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct
    16141 ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt
    16201 atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact
    16261 ttatatgtag aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt
    16321 gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac
    16381 aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc
    16441 tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac
    16501 aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct
    16561 gccaactccg cggtaataag tccagctaaa cgaaacatat gaagagctat tttttatttg
    16621 actcttctta ttacttgatg cagaacagct ttttcacaaa atttttcttc atactcaaaa
    16681 ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt tcttttaatg
    16741 ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca gaggaaatag
    16801 ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct gcttcttgag
    16861 ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca ataatttcta
    16921 actctttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca gttagattgc
    16981 cggataattc taagatttct ggagagtcac tttctactag tacttttgct gcagcttctg
    17041 catattcacg tttcaatgcc caacctgttt ttccatttcc tgcagcataa acgaattgac
    17101 cagtttttaa agcctgccca atgataggta attcattctc tacataccaa ttattccgta
    17161 aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg tgatctggcg
    17221 ctaatacact gatcgagtga tcggcatctg caaaacttgt ataagcgata taagataccc
    17281 cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt cctggaacgc
    17341 cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag tctaaatcat
    17401 cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct ttttcttcac
    17461 tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat tccagcgcat
    17521 aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc actccctatt
    17581 ctttttattt gttaagattt acagctgtac atttataaat tacaccttta tcataaagag
    17641 tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa gtattctctg
    17701 caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga tacccgatca
    17761 ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc
    17821 atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc
    17881 cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat cgaaggtaat
    17941 acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc
    18001 caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct
    18061 aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga aaaaagacca
    18121 gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct gcatttttct
    18181 gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct tgtcatgccc
    18241 ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg
    18301 ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa agctatttcc
    18361 catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta tattatcatg
    18421 gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta actcccattt
    18481 cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt aaaacatgcc
    18541 caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct aaagcgctta
    18601 caacaagata cgttcctttt gggaatggga tgattcgcgt agtgtttgga acatcctgac
    18661 tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg actgtaaaga
    18721 aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg ataacttttt
    18781 gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt
    18841 ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac tttgccattt
    18901 ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga aatgacacga
    18961 ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct ttcactgcaa
    19021 tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa gagatctcgg
    19081 gagcttcgac taacccataa tctgaagaag gaagccattc tgcatagatt ttcccccaag
    19141 tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa gtgtgttctt
    19201 ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca aagccaatca
    19261 tgtgcatcaa actgcccttt tcttccaagc ggtctccatc aaatccataa gaaacgttga
    19321 ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga gtctccgtga
    19381 tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca ggaacttttt
    19441 tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc atcgattttc
    19501 ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg ccgtatctgc
    19561 tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg
    19621 tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctaga agatcgcgat
    19681 tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct gcaataaacg
    19741 taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt attttatagg
    19801 aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga tctttaagca
    19861 tatttatctt cctccttgat tgccttttta tcatattatc aaagaaagtc aattacttga
    19921 tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa agaaaattct
    19981 caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt tacctttaga
    20041 aaaataaatt ttggggaaga cagattctaa gtaaacaaag cggtaaaaat atcttttatt
    20101 cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca aactgctgat
    20161 cagaacgctg cttggatttt tctttg
//
LOCUS       DAZKAY010000053        19507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000053 DAZKAY010000000
VERSION     DAZKAY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.053"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8WP_001728"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8WP_001728"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9028033.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8WP_001729"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8WP_001729"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9028034.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8WP_001730"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8WP_001730"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9028035.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8WP_001731"
     CDS             2927..3532
                     /locus_tag="ACG8WP_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9028036.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8WP_001732"
     CDS             3857..4180
                     /locus_tag="ACG8WP_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9028037.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8WP_001733"
     CDS             4362..5732
                     /locus_tag="ACG8WP_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9028038.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8WP_001734"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8WP_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9028039.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8WP_001735"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8WP_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9028040.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8WP_001736"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8WP_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9028041.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8WP_001737"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8WP_001737"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9028042.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8WP_001738"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8WP_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9028043.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8WP_001739"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8WP_001739"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9028044.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8WP_001740"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8WP_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9028045.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8WP_001741"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8WP_001741"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9028046.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8WP_001742"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8WP_001742"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9028047.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8WP_001743"
     CDS             16214..16510
                     /locus_tag="ACG8WP_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028048.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG8WP_001744"
     CDS             17078..18670
                     /locus_tag="ACG8WP_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9028049.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG8WP_001745"
     CDS             18683..19423
                     /locus_tag="ACG8WP_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9028050.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZKAY010000054        18742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000054 DAZKAY010000000
VERSION     DAZKAY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.054"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..1038)
                     /locus_tag="ACG8WP_001746"
     CDS             complement(127..1038)
                     /locus_tag="ACG8WP_001746"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9028051.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1415..2053)
                     /locus_tag="ACG8WP_001747"
     CDS             complement(1415..2053)
                     /locus_tag="ACG8WP_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028052.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2057..4039)
                     /locus_tag="ACG8WP_001748"
                     /pseudo
     CDS             complement(2057..4039)
                     /locus_tag="ACG8WP_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4219..4884
                     /locus_tag="ACG8WP_001749"
     CDS             4219..4884
                     /locus_tag="ACG8WP_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028053.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5281..7314)
                     /gene="brxL"
                     /locus_tag="ACG8WP_001750"
     CDS             complement(5281..7314)
                     /gene="brxL"
                     /locus_tag="ACG8WP_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFP9028054.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7432..9954)
                     /gene="pglZ"
                     /locus_tag="ACG8WP_001751"
     CDS             complement(7432..9954)
                     /gene="pglZ"
                     /locus_tag="ACG8WP_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFP9028055.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10070..13702)
                     /gene="pglX"
                     /locus_tag="ACG8WP_001752"
     CDS             complement(10070..13702)
                     /gene="pglX"
                     /locus_tag="ACG8WP_001752"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFP9028056.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13766..17437)
                     /gene="brxC"
                     /locus_tag="ACG8WP_001753"
     CDS             complement(13766..17437)
                     /gene="brxC"
                     /locus_tag="ACG8WP_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP9028057.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17462..18037)
                     /locus_tag="ACG8WP_001754"
     CDS             complement(17462..18037)
                     /locus_tag="ACG8WP_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFP9028058.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18037..18636)
                     /locus_tag="ACG8WP_001755"
     CDS             complement(18037..18636)
                     /locus_tag="ACG8WP_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFP9028059.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5790 a         3568 c         2621 g         6763 t
ORIGIN      
        1 aaaattttta aaataaaaag aactaaatca agtaataatt aaactactgt tgatttagtt
       61 ctaataaaaa gatttagatg aacagtttta ttgatttaaa tagttcatat tttttattta
      121 taccgcttat ttactacttg ctgtactaga atctgaagtg aatgtactca aaacattttc
      181 aaagtctttg tctttgattt ttacattagc gtctttcaat acttcgccaa ttactttatt
      241 ctggaaggta ctgtcactta attttgtgtc tgttgcaatt tctttcaatt cttttttgta
      301 tttatccatg tcgttgcctt tgttttggtt tttcaccatt ttaaccacat aatattccgt
      361 agtataagtt gaagcattgg tagaagtgat cacatcagaa acttgaccat ctttcaactt
      421 aaaggctgct tctttgactt ctgccggaac cgttgtagta gtggaatcaa atttcacttt
      481 tccgccatct tcttttgtcg tagtatcagt tgacttgtct ttggctaatt tagagaaatc
      541 gtctccctta tcagcagctt tcttcacatc ttttgcatca tcttcacttg ctacttggat
      601 aatttgagct tccacttctg gatggaaaga ttcccaagcc gtttttaaat cttcatcggt
      661 caatttgata tgtttcttta gaccttcttg aaaggctaga ctttgtttga tactgtcttt
      721 gaatgtttgt tctgtgtaac ctgcagattt caattgtgaa tcaaatgaat cacccaattg
      781 tttctttgtt tgatcatatt gggaatcgat ttctttggtt gatacgtcat ctccatattt
      841 atcttcaaat acttgataaa cgatcaactg ttgcacagcg gaatctgctc cttgcatttt
      901 tttcaagcta tccattactt cttgttcgct gattgtaccg cctttataac taacggctgt
      961 tttttcaccg gcattcgtac agcctgacaa taagccagtg cctagtacga tggcaagtgc
     1021 taaaaatctt tttttcattt atcaattctc ctacttttcg ttttttgcga attgctaaaa
     1081 acaaaggtgt tcaagatgaa aatcatcaag aacaccttta ctaatctgct cgatacgtcc
     1141 tgaatagata taaggctaca caatccagaa agttcgctca gcagaataaa tgtcaagtag
     1201 cacatcacac cttaatatac tacttctttt gttaccgttt ttcaagtcta tcttgcgttc
     1261 cgtatttaac gtaactaatt cttagaacaa atgttatcga ttttgttttt cttcttccgc
     1321 tgttgggata ttcaaatcag acacttttat gttcgatttt ccagacattt ttttgttgtt
     1381 tttattagaa aaagtttgtt ttttcatttg ttttctaaag catgatcttc ttgtttccta
     1441 ttttggtcag atatggatca tgcgtgacca cgataactaa cgtaccttgg gaagcctgtt
     1501 gcgataagat ttcaacgact tgctccttat ttcgtttatc taatgatcca gttggttcat
     1561 cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt ctttgcttct
     1621 caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt acttttttca
     1681 atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac ttcaatccaa
     1741 tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga aaaagaaaag
     1801 agagggcatt tctttttatc aagcgcttct ctcttccagt taaatgtttt gtttcttgat
     1861 taaataaata aaattgccct gaactgaatt gatcattcaa tcctaatata ttcaataaag
     1921 tacttttccc tgctccgctc tctccaacta cagtcacaaa atccccagca tccgcagtga
     1981 aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct tttatcacta
     2041 tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc ttttctgtag
     2101 acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa atattaaaaa
     2161 gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt ccccatttga
     2221 tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca cttgaacgaa
     2281 agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca tagctcaaca
     2341 agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat gatctgctta
     2401 acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc aagttatcac
     2461 gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag cctatcttga
     2521 tctttaacgg tggatagatt tctccgctga gaatatttct ttcccaatac gtactgtttt
     2581 tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa ggaataaatg
     2641 tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca ctgatttctt
     2701 gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta tatcgttcag
     2761 ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt ccagagacat
     2821 caatcaaagg aaatttctta agataattca gatttatttt tatccctctg ccaaactgtt
     2881 cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt ttattcaaag
     2941 tttcatataa ttcctgatcg gctgctgatg caaaacgatt ggaataaata aaatccacgt
     3001 ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg atttcttttg
     3061 gtgtttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct aattcgacat
     3121 tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg tattggatat
     3181 agttgaacgc ttctcttaac gaagcgattt tttcaactaa taaaagagaa atcaaagaga
     3241 taccctatat tccgctgaac aagaaaacgt acatctggaa caaataaaga aattgctcct
     3301 tataatacat aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta ataatgattt
     3361 tctttttccc caataaacac cacaatactt gatgtaatga aaagccatta ttcaacaata
     3421 aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat aaaagaatca
     3481 atccaatagt taaatacact cgataattat atacgtctgt acttgaaata accgaatacg
     3541 aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga aatgcttgat
     3601 tgaatcgttc cacaaatagt aaaatgaacc gttcataggc ttctttttct ctcgtcatca
     3661 agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac gttacgcgaa
     3721 aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct tgataataca
     3781 atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact tcattggatt
     3841 catttactgt ttttttcata taagaaagct cagtttctga tgagacattt tcaaatacca
     3901 tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga atcggagtat
     3961 aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta tttataataa
     4021 ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact gtgaactttt
     4081 ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca taccgttttt
     4141 catcaaaaaa acgtatctat caaatgtata ggtcatgtag aataaatcta tatactgtag
     4201 aatcgaaagg agaagaagat gaacatcgga gaaaaaatac gaacttaccg cagaaattgt
     4261 aagcttacgc aaaaagaatt aggcgaaaaa atcggtgttt ccgataaaac aatctctagt
     4321 tgggaaaatt ctcgtacaat gccagacctt gaaatgctgt cacttttaca tcagacttta
     4381 ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga accaacattg
     4441 ccggatgaac ctcttgttat caagaaatct tattcatcaa aacaaccctt ctcactcaaa
     4501 aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct tatcaattat
     4561 caatataaaa atgaaacggt tgatcgattc aatccttttt tgaaaccaca aatcggctat
     4621 accacactcc ctgtaggata cgtggaaaat tccatcgaac attgggtatg taatgatatc
     4681 tttgttcatg gtacatggct gtcttttcac gctggagagg tagaaaaagg taagaatcat
     4741 gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc
     4801 attccctcag attaccaaga aatcctcatc caagactatg acgatgcctt aaaaagaagc
     4861 cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa aaatcaatgc
     4921 acatgatctt atcaaaaaaa aacaggggta caaattaatt gaaaaagctt tgagatgaaa
     4981 gttcctaaat aaaaaagttc agaatgatag gtaaaaaaat gactaactat tctagtatta
     5041 ggctatttgt attattcttc tgttattttt atatcaaaaa atccagaaat aactaaatat
     5101 aggtatttct ggcttatagt ataatattta cgccacagcc cccaatgatt ctttttggaa
     5161 tcatgatcaa ggacggacat acacaaaaat attttgccca ttcgatccga atagattaac
     5221 gactaatcta gtaagtaata tagattggta cgtagtcaaa ccagcaaatg gaaaagttgt
     5281 ctattccacc ccaagggctt taaagacagc atcttccggc gtttgataaa aaatcaaatt
     5341 aaaggtaccc attaattctg ggggtacagt tccaatatca gcagcgctta tcataggtac
     5401 aagtactttt ttcgctccgg catcttttgc tacttgtaaa cagttagcta aattatctac
     5461 cttaatcata gaaccaccaa ttgtaatatc ccctaaaata actaaacttg gtaatacagg
     5521 tttatttaag gaaactgagc ataaagctat tatagttgat aacgataatg tttttgtcat
     5581 accaatgcca ttcatatctt gaacatgaac gacaaaatct tttgtcgtca aactaataga
     5641 ctgactaaca cttccataat tagctcttaa atatcggtat gccgaatcca tcgcttctct
     5701 tgctccagca tctgaattga tcccagtacg ttcaaacttt ccatttcccg aagtcatttg
     5761 cgtttccaat ttataggtac ctaattgccc agttttgcca taagataccg tgtacaaatg
     5821 ccctggcttc cctaaccctt ctggaatcaa agttcctcca ccttgttcag gaacagaaac
     5881 atactcttct cgctcttctt ctaaatcaat gtatgaaaaa ttgacatcat aaaattccat
     5941 tccaccaatt ttctttaatt gttcttttac acgacgacgc atttcaagag caaatcttag
     6001 tatttcttcg acatcacttt tttcaaatct accatcagga tacaacagtt tcatcatccc
     6061 agatacaatc cgacgaactg caatcgtatc ccgctgattt aattgagaac caagtttgaa
     6121 atatttatca aaactatcgc caaaactaac ttttcgaagc tcacgcatga cttcagaaaa
     6181 ataatctgta atgaagccat agtcatttgt aaaaaaatct ggtcgatatt taggaatttc
     6241 ccaacctggt aaataacaat gcatccgatc caaaaaggct gaatccgtcc ccataacctc
     6301 cggaaatggc tcaaataaat tcgaagtttt caatattaca tcaacgcttt gattaatatt
     6361 tcctacaaaa gccattgatg ctgaagcatt cttttcttct ttgcctctag caaaagatcc
     6421 tgaagccatg taatctttca tgatttgaac gccatcatta tccttaaatt taatcccagc
     6481 aacttcgtca aaagcaacac agtcccacat acctacaagt cccattgtac gactagccat
     6541 attatagaaa aggtttgcta ctgttgtttg tccacctgaa acaaggattg agtttggtga
     6601 aatttcttta tatacatgcg atttacctgt accacgtggt cctagttcac agagattaaa
     6661 attattttca attaatggaa taagtcttgc taaatgaagc cattttaccc ttttattaaa
     6721 ttgtgtaggt tccataccaa tggaccgtag aagtaagtca atccattcat ttgtagtaaa
     6781 ctccttgcga gccatcttgt aagcctcaat gtctaaacca ggcatttgga ttggatcaac
     6841 cttttttgca ataaaagggc tattccaacg atcattctca tcataccaat attccatcga
     6901 aacaatacac cagatccccc cacctaataa tcgatcaaat tttttcggat actcttcact
     6961 aatttgaaca ttttttaatc ccaggttaga gaattctgct tcataacaat ctttcttttc
     7021 atttaaatgt acagttactt tatcaataac tgtataacca cccaattcac gaatttttgc
     7081 ttttactttt tctgcttcat caggacgcac atagtttcga gcaagaatat ttttaacatt
     7141 ttccacacct ttttcaacca atgtttcatc caatgacgaa gcatacatac caagtaaata
     7201 ttccaataca taaacaggta cattcgcacc ttctttaatt ttctttgtta aatcttttct
     7261 gacaatcttt ccgggaaaga attcagataa ctttaaaatt tcctgttgtt ccactagaat
     7321 tcctccatgt gttcagtcat tataatagca attaaagtat atcatactta accagtaggc
     7381 ttgataatac aaaagaaaaa gtatccacaa aaaattgtgg atacctcgtt tttatatatc
     7441 aaaatcaaat gattctgcaa aaggattatc tataatgaat tttattcgct cggtcaaatc
     7501 atttttttga ttttttatca ctaaataata agtatcttca actgtatatc gacgatttac
     7561 aaattcaaaa acaaatttag taaaccgctg atttgcatct tctgtttttg aatccgcaac
     7621 atagatttct tcatttgaga tacgttcacc atattcatct tcaaaataaa gccagaaaga
     7681 tgcggggtca taaacatcac taattgcatt tttttgatag aattctaacg tggttgataa
     7741 tcctgtaatt ctgcggttgc tagtcattaa ttcaattgct actggttcct ttgcattgct
     7801 acctttggct accgtaattt ctagaacagg gactagcatt tcttgaatag atgaccctcc
     7861 atgaacgtag ctttgtcccc cgccagcttt cttaaaaata cttgaagtaa tcgggacaca
     7921 aatgtttcta gtgtcatcat ttcctaaaga atctcccaaa gagtaatttt tcgtaccaat
     7981 tatttcgtat tcttcttcac taagaataaa acgacgttct acacgatctt tttcatttct
     8041 agatggattt tcaatcttat ctacttcttc aacgtggctt cttttataaa taaatccatg
     8101 atcagccgta actataaaac gatagatact gccatcatta tgcaagcgat taataagtcg
     8161 ggaaatttcg cttatcgcta aatcacaggc ctcaaaaact cgattttcat tcgagtgatc
     8221 acctgttcta tccacttgat cgtgataaat ataaatgact gatttgccat taaatacttc
     8281 tcgtacctca gccttattca tcttaaccac atcatcataa tttaaagcta cagcatttgc
     8341 atctcgttgc tggagaatcg catttcgatt ggataaacca ttcgtttttt tcccatcaat
     8401 cagtacttct gttccttcat tgtaagttag ttgattgttt ggtaataagg cggctttacc
     8461 gaattcagta actgaaggta atacagaaag aaagctagcc atgtttgttg aatacttctt
     8521 ttctctaact aacttacgtt ctaaattcat ccctaactca tatcgaaaag catctgaaat
     8581 aataactaca gttttaacag agcgtttcat tacagcttga ttataaaatt ttcgaatgga
     8641 tggacgattc ttaaattcaa aagtttgatt ccaaatttgt cctgattgat caagaaactt
     8701 cgaataattt gcttcaacta atcgatacaa ttggccatat ttttgctttt cttctatatg
     8761 atcactattc caaataaatt tccgataatg cgtatcaacc caatacattt ccttctcata
     8821 ttgagttagc aaattatcca tgttcatcgg caagcacaat ctgtttttat ccacaagata
     8881 ataagcatgt ttcaataatt gatactcata aatataatcc gatgcgaaat gcattttttc
     8941 gcgctgcttt aaactatcaa aaattgtttg tcctgcaatt ttcgttgtag tgtcagaagc
     9001 ctttaatcgt tctgcataaa acattaatat ttttgcatga atctcagaaa atacgtctgc
     9061 ttcaattaga tcatcaattt ctatactgtc taataatcca tcaccatcaa tagcctgata
     9121 tactttttta gatagctgtt caaagctttc catgtaacgt gtatcattca taaactgatc
     9181 catgaaagta ataatgtttg ctttcaaatg tagtatttca aatcctttga acttcttcgg
     9241 catctcttga tcaatttgat aataaaaata gttcaaataa agacacatcg tcagcttttc
     9301 caatgttggc tggctatccg taaagccaaa tttcatttga ataaattccc aaaatttagc
     9361 tgttaactga tacttatcaa gttcccttaa aggattctcc ctactttctg taaagctttt
     9421 tagaactaca cgcagtaaat catttaatcg aatacttttc gatttcaaca aaacagccat
     9481 tatcattaat tcaatcgttt ctgatttatt aattgtcata tgcgtactaa attcaccaaa
     9541 acttctcatg cgatcctttg cattaaagaa attacgatac tgtttaactg tctgatgaaa
     9601 tctaagtgga atagccaatt cattcatgat aatcgctaag cggtctgcac taaattcctc
     9661 tgaataattc aatacactca acaaatgatt atcttcatct ttatcatccc aacgaggaaa
     9721 tggcgcatag attaaatagt tagtttcggg atcttcatat tccaataaac gcttagtttt
     9781 aaattgtgcg tccttttcta atcgatagat ttttgcattt tgaatgacca atgagtcaat
     9841 ttcttcaaga aaatcctgat ttgaatcaaa ccaaaaaatg attttcctac atccataatt
     9901 aggaaaacgt tgatttaatt tttcttcaat ttgttgcgta tttatatctg tcatcaatct
     9961 cacctaatcg ttctctttct atacccctta tattgacata atttggataa tttcgcacgc
    10021 aatttaagaa taaaaaagaa agttgttaga aaacaacttt cttagataac tatttttttg
    10081 gttctttgac cccttttgca aaaatttgga atttctcgcc ggttttagga tcacgctgca
    10141 acttatcata attgacaatt acaccatcat ctaagtctaa ttcaattcgt tttaatgcta
    10201 agtgacccaa cttctcgtca tactcacgaa cttcttttaa ttgcttctga atctttacaa
    10261 tttcttttgt cattgcattt ctttcttttg ggttatcact tgttgaaatt tgttgctgac
    10321 gcaattcgat tcgattgtca tacgcttttt gtagttcgtg taggtattca gtacgtactt
    10381 ttcctaattg atctggtgta tagcgatgca agtacattaa cgctttaaag ccattttgtt
    10441 ttcctgagtc caattgccaa taaattggtc gattggagta tgtttttaaa tggtctttgt
    10501 aaaaatcttg tacgaagtaa cgacgaataa tttctcgact tgtccctttg ccacctaaag
    10561 tgtcagcgat aaactggaga ttttcttcaa gggtttcttt accaaaagta atctcaacga
    10621 aatcaatgaa acgattgaca atgtcaattt ggctatcctt gaaatactca tcatcggtga
    10681 tgagtaaaac attatcagta tcaggtttaa atttttgata gcgggaagga tcccattcgc
    10741 cgccagcgaa gatgagacca tcttcgtcta gggagtaacg accgaagata caacctattg
    10801 aataagaaat aaaatcctta atttccctcg ccttatcagg taaccttgca gagatatatt
    10861 catcttgaac ctcttttgtt aattcttttt caagagaata atgattaatg aatatgtcgt
    10921 tcagttcctc ttcattcgat tttagagtat taaattgatt aaccaactgc ctacttagac
    10981 agttaaatgc ctctgagaga tcttttttaa acatataact taacaaaatg tttcttctaa
    11041 actcgtatga cgtttcaaga gcattatagt cttttctagc tatatcgata ttttcatcca
    11101 ctttttctct tatttttttt aattttctct cagatgtaac tttaagaaaa ggtagttttc
    11161 ccattggtcc ttcatggtaa tccaaggtgg gagaaataaa agttaaaaag tgattaacta
    11221 atttagagtt tacatagcca aatatttctt caaagtcagt tatatccttt gagaacagaa
    11281 cagatccttt agtttcaaaa attgcctgat tctgcaatct aaaagatact tgtccagatg
    11341 aaagtgtcga ccaagtaatt ccctttttaa aatagtaatt aagattttgt ggtctagatt
    11401 ttaacttccc attttcatta acataatttt ttatttcatc tccatcattc tcccagttaa
    11461 ccacgtattc attgtttcca taccattttc taaaacgacc acctttagtt gtaggaaacc
    11521 atttttttct agacttttta gcgtcctcta atgttttagc atcaaacttt attttgttaa
    11581 attctacttc aaaccaatac cgcaaaaatt tattgttatt tccggttgcc aacccttgtt
    11641 ttgcatctgc aacatcgtta gttaattttc cttgagcaaa atcagtgatt acgttatcgc
    11701 ttgcccagta agcaatcggc attcctggga tttttttgaa gttgtcttga ttagtactat
    11761 aaaaataatc gcattcacca tgctgatgaa ttgcttctaa cgttttaatt ttttgaactt
    11821 ccattccacc tttaaaatct gataatttta tatagcttcc atcttgttca tttgaatttt
    11881 ttatcgtaaa cgtacaaatt ggaacggttg cttcttcaaa agctgaatac tccatttgaa
    11941 tcaaactaga aatgtttctt tcattaatta aaaatgagcg taacttttca tagctagaaa
    12001 tgaacatcca aacaaatggc gtcataaagg aagcatatcc attatcaatc agcatttggc
    12061 tattcaattt aataaacaca gaaaatagat cttttttgac atcagaatag ttatcattta
    12121 catatttttt taatttctta tccattttat taagataagg cggattagtg actaccgcta
    12181 catatttatc aactagcatt tcagccacgt caagaatgtg caagtatttt tcttgtattt
    12241 ccttcatccc ataaagaata tccatattcg taaattcgtt gaattttgat atatactctc
    12301 ttacttttcc taagtattct ttagttattg cctgattatt tttatcgact aacttaatga
    12361 ttgatccaag ttctttccca ttttcaaatt gatcaaaaat taactctttg tattcattcc
    12421 agcgttcact atacttatca ttgttcaatt cactaatacg ctcttgatac tcgtctgtca
    12481 ctttaggaga atcaataaac tctcttaaat tcggcgatag actttttttc aaaaaaagac
    12541 gatcgtattg acgccctttc atcataattg caaaataaga caattgatac gctcttttat
    12601 cgatttcaag accatataaa ttgtattcaa cgattgcacg tgcagcatca cgctcagaat
    12661 atcctaacgt aagataaatt tccatcaata catcgaaggc gtaaactaaa atatgcccag
    12721 accccattgc gttatcgata atttttagtt cttctggttt taaattactt acagatgttg
    12781 cttgttcaaa agcatattta tctggaatta agtatttcaa cttatttctt aattcactat
    12841 tgggataatg atctaaccaa tactttccta aactattgtc aaccatataa cgaacgaccc
    12901 aatctgtggt aaatagttga gttgctgccg gaatatcttg ttttttgaca atccctttat
    12961 aaatattcac cacttgatta tgtggctcac tattatagta ctgatacaac cacccaataa
    13021 tctctacctg tccaccagct tgaacataga aatcatcctc tggaatactt aaaagatcag
    13081 cgattacacc atctttttta atataagaag ctgtaaacaa aagttctgta taatcttcta
    13141 cttcttcaaa tagctcaggt aagtaatcat tcaattgatt acagattttt ataaaaatca
    13201 attgatacag cttgtccatt gcttctgctg aattatctgt tttccaagtt ctgacttgtt
    13261 gctgttcatt actagataat tcttcaaaga tatcggcatc ataaatatct gtaataatat
    13321 ctggctcaat tttcccactt atttcactag ataatacacg atacgtgtca ggtaaataat
    13381 catttacctc cataaaccga atggcaatca atcggttaaa ccacgtataa gcaacctctt
    13441 cgattaactc ttgaaaagct tctgaataag aagtgacaga agcacgttgt tttagttctg
    13501 caatcaattt gtttctttgc gctatttctt ttccttgaat tcttattcct gatttttcga
    13561 tttcttgtaa attgctatca attttcttca catcttcaat ttttgattca tcaattccta
    13621 aacgttttgc tttagtcatt acttcatcaa ttaattttct acgggcattg accgcgaaat
    13681 tctttatcgc tttcttatcc atttttcttt cccttctata tgtaaccttt gaaaagacag
    13741 gcaccttttt atgcctgtct tttcactaga attgaatcgt gatttctgta ttttcttgaa
    13801 gttgttcctt cagtttttga ttaatttcag ctgttaactc atcaatatct gctgctgact
    13861 taatagtttt aacatgaacg ttaagtgttc tcattggtac tgttttctgt tgaatagtag
    13921 gttttactat cgctatttct tcagcttttg ctgcagagtc tatttcatct ttttcagttt
    13981 cacgaatcaa gtttgctgct tcaatttgtt tacgtgcttg ttcttcttga cgtctgcggt
    14041 tttcttcatt atcgaaacgt tgattaaaga tcaaatactg ttctgctacg tttaatgata
    14101 agaaaacaag atcatctata gtattcgctt gttcaatttt tgctttcagt tcttgaaatt
    14161 cttggtcaac tattggtaca aattgatcct taaaatcttt tccttctaat aatttaaatg
    14221 ttgcttgttt ttcttccgtt aatttattta atatttcctg actttccaac tctaacagtt
    14281 gattatattg ttcaataaat tcttgaatca actcatttag tttcatcata tctgaataag
    14341 gatcagcctt tttcaaaatg gttttcatct catgaatcaa ttgatcaatt tcttggctag
    14401 aagtttgacg ccgacttttt tcgattaatc taattttatc ttgtgcattc tcccagattt
    14461 ttttctgagt actattttgc ttgtggaaat ttttaatttt atcatatgtt tcagcccagt
    14521 cgaataattc atcgcgatat tttttgattg tataaaaaaa gtcttcaaca tctgacgttt
    14581 gaacaattac ttcaagatat ttttttattt gctcatattc ataagcacta gggtaggtat
    14641 aagtgttttg taaatcaaaa taagagcgta aattgtctaa ttgacctttt aaatatttct
    14701 tgaaattttc cattgtatgt tcattgttat tttcatcaac aatattacga ctttgaaaca
    14761 gttctctacc gatttcgtca accaattttt ttgcttttgg cgaaatcacc gcgcttggtt
    14821 caatttttat cttttcactt tctcgtcgtt tttgaataat cattacaata ctatttggat
    14881 catccatccg tgaaatttgt acattgttat aaagtaaatc aattttacca gacatgaaaa
    14941 gtttagcgat gcaccattcc acatcaagtt ctgtgaatcc atacggttgt tttgtttcaa
    15001 aacgatcacg aattgttttc atcgtaatcg ttaggtgatt tctcttttga tcattgataa
    15061 aattgaaaat ttcctgaact gcttgctcat ttgatggatc ttcaataatt tcatttttgc
    15121 tttctttcac taaatataag atatcttctt tttctttagg cgcatcaatt tggtttaatt
    15181 tatggtatac ttcttcagcc attttctgaa gtgctgattg gaattttgct ttaaagtcgg
    15241 agcctgtcag ttgaatttct gcaccgttat agaaaaattt tgcagatttc aacagttcat
    15301 ttaattgatc gttgatactt gcacttagtt cctgtaattc accagatttt tccgaacgaa
    15361 tcgcgtcaaa gttctttact acactcgaat aagtcgttgt taaataaaga ttcattctag
    15421 catacattgc tatatcatct ttaaattctt tatctgatgg caattctacg aataataagt
    15481 tacgggaact cttaagcgca agttctgcct cttgacctgc taaatcagag tatggtgaaa
    15541 taaaattaac tgtcatttca gcatttccag cttgcttgta tggacgttga tctaacactt
    15601 gtgcataagt aaacgtatac cggtttttaa atttatttcc acctgacatg cctaattttt
    15661 cataggcaaa actatttcgg tctagaacgc cgtcaaaaat tcgtttagat agttctacga
    15721 tcaaatcact atctgtaata ttttgatttt taatctcacg agtaatttcc tgttcttcat
    15781 cagtttggaa aatataacta tctaagttct tccgcacaaa gttttcctta attaaaaggt
    15841 ccaatgcttc ttctactcgt tttttagttt ccaggcggtc ttcgttgata tttcttatca
    15901 tcaagctcgt aatattttca acatttggtt tgatggcttg atcaacatat ttaatcatga
    15961 ataaaattcg taagacattt acgttaaagt tatctatatc agttggtctt tgaatatgag
    16021 aattatttgt agcttgtgta atcactcggc taattgctgt atcaatccaa cgttctaacg
    16081 agtaataaaa ctgatctaaa gaaaccaaac tggttgtatc attttcctta tttcgttcag
    16141 cggcttcttt aaacattgct agtaatgaac gttcaccaga cgataagttt gatccttgat
    16201 agctgtgatt acgaatctcg gttagaattt gccccaataa attgaattga tagggaataa
    16261 atggataaac ttctgcgtaa ttttcagcat cttggaagag ctttaattca ggactatcaa
    16321 caaagagtaa tttatttttc aattctgttt gaacagtttc aaattgtaag gctaaagaat
    16381 cattggcaaa ctcttttttc ttcaaaatac gttctttaat tacttcatcg gcatttgctg
    16441 aagataaact cacagttgtg gcaaaacgat ccttgatctt agaaaaatca ttatcagaat
    16501 tgttatcaaa gtttgttacg tgatcaatcg cttcctgact tgtcactaat acccaagcct
    16561 taccttcaca aattcgaccc aaatcttcca caacggtttg taactgcaaa gtcaactcac
    16621 gattaccgcc gatatattgt ccgacctcat cgactagaaa ggcaatatga tggtctgctt
    16681 ctttggaatc aagatagctt ttaactagat ttgcaaagtc atcaatgctt attaaataat
    16741 ttcctgatgc tttttctaaa aaattatcaa tagcggcttc actcataaaa tcgattttaa
    16801 gtgccgcttc tttgatatca tcttggatcc aatcaaactc atggcgctct tcttgccatt
    16861 ttgccccatt aatctcttca aaagcagctt tgaatgcatc aaacttttct tctttaatta
    16921 accgacgttc aaaatctgct agatgtggaa tcgtgcttaa tccttgcatt tcgttaaata
    16981 cctttaaaaa aacattcacg attccgtttt tgtctttttt accacctgca tcactttttg
    17041 aatcaatatt aaacagaata acatctgtat ttatttgtga gcaccgttcc atcgttgcca
    17101 aagtcagtgg atctttgatt ttcccatctt ctttgaagta atcaatggct ttcttgtcat
    17161 ttatttctcg atttgcgagc aagtaagaaa taatttttaa gaaatgagat ttacctgaac
    17221 caaagaaccc ctgaatccaa acacccattt tatctgttgg tgtattgata ctccgttggt
    17281 aggcatcaaa aattgtttta aagtgttttt gtagttcttt cgtaaccacg tattcttcta
    17341 attcttgttg aacattttca tcctttgctt gaccgacctt aataactcct tgaatatttc
    17401 ggtcaattgg ttttgcaaaa atatctttaa ttagcattta atctcctacc ttccatccca
    17461 actaaattct aaatgctcga taataattat catctttatt ggtattaaga attgtcaaac
    17521 ttaattcatc atattcccct ggatacatca tcacaactgg tacgcgatca aatacctgat
    17581 gcattttatt taaaatagag tgcgctctaa ttaatggata taattcgcca attcctgtca
    17641 ggaacacaac tgatttttct ggtgtatggg catccacata ttcaaaaaat acattttctt
    17701 cctcaggtaa atccattttc aataaacgac caatgctttc cactaatttt tgtaaaccac
    17761 gtcgttcttc catcttaatc gctttttcag tgaaaccgcg tttttctaga tactctaaca
    17821 taatcacata gagattataa actttcactt cataattatc accaacagat gttccagata
    17881 tatcattaat ataatctctt acaattaact cgtctttcgg atcgtagtca aagatataat
    17941 agcctacttc attacctaaa ccacgattca ataaaaaatc ttctttttga attacttcat
    18001 tcaacttttc caaatgctct tgaatcgata atgacattat aaaattcctc ccagtgctag
    18061 tacaatatct ttacgattct tgtcaactaa ccattgaatc aatcgtttat ctaataatat
    18121 cggagtcatt cgtccatatt ctttttcccc tctgactaat cctgtttcaa ttagattcgt
    18181 ttgataagcg gcaataagcc gtttaatcgt ataatctttc cattttgaaa tatcttctga
    18241 acttctccgc ttctctgtaa aaaaagaacg gtaatcaact ggtgttaatt gcaaatttcc
    18301 tttacgtaga ttttcagcat atacctcagt cataaattca ttaaaaagat catcaatcat
    18361 aaggatggaa atcaaattca ctaacttttg attatctata tttaactgat tgaaaagttg
    18421 caatccagct tcatccagtt gactaactct ccttttcacg ttctgataga tttctttttg
    18481 acggttactg ctcactgcaa aaaaaatatt ttcatgttca tttttctctt taatttctat
    18541 atctgtatat ccttctagtc gtaattgaat atataacttg aactctgaaa accaaaagcg
    18601 tttagaaact agaccagcag aatatttttt attcaataga ttgcctccta atcatctata
    18661 attcaataag taaacaagtg ttttttgctc atcgttagca ttcatttttt agtatctact
    18721 atacaataca tatttattat ag
//
LOCUS       DAZKAY010000055        18501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000055 DAZKAY010000000
VERSION     DAZKAY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.055"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACG8WP_001756"
     CDS             72..155
                     /locus_tag="ACG8WP_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9028060.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG8WP_001757"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACG8WP_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9028061.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACG8WP_001758"
     CDS             complement(1280..2143)
                     /locus_tag="ACG8WP_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9028062.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACG8WP_001759"
     CDS             complement(2145..2417)
                     /locus_tag="ACG8WP_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9028063.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACG8WP_001760"
     CDS             complement(2434..2643)
                     /locus_tag="ACG8WP_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9028064.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2742..3638)
                     /locus_tag="ACG8WP_001761"
     CDS             complement(2742..3638)
                     /locus_tag="ACG8WP_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028065.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(3808..4301)
                     /locus_tag="ACG8WP_001762"
                     /pseudo
     CDS             complement(3808..4301)
                     /locus_tag="ACG8WP_001762"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4377..4508)
                     /locus_tag="ACG8WP_001763"
                     /pseudo
     CDS             complement(4377..4508)
                     /locus_tag="ACG8WP_001763"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(4784..5578)
                     /locus_tag="ACG8WP_001764"
                     /note="aph(3')-IIIa"
     CDS             complement(4784..5578)
                     /locus_tag="ACG8WP_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9028066.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5671..6213)
                     /gene="sat4"
                     /locus_tag="ACG8WP_001765"
     CDS             complement(5671..6213)
                     /gene="sat4"
                     /locus_tag="ACG8WP_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP9028067.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(6210..7118)
                     /locus_tag="ACG8WP_001766"
     CDS             complement(6210..7118)
                     /locus_tag="ACG8WP_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9028068.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(7151..7885)
                     /locus_tag="ACG8WP_001767"
     CDS             complement(7151..7885)
                     /locus_tag="ACG8WP_001767"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9028069.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(7866..8735)
                     /locus_tag="ACG8WP_001768"
     CDS             complement(7866..8735)
                     /locus_tag="ACG8WP_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9028070.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(8750..8974)
                     /locus_tag="ACG8WP_001769"
     CDS             complement(8750..8974)
                     /locus_tag="ACG8WP_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028071.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(9117..10661)
                     /locus_tag="ACG8WP_001770"
     CDS             complement(9117..10661)
                     /locus_tag="ACG8WP_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028072.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(10681..11097)
                     /locus_tag="ACG8WP_001771"
     CDS             complement(10681..11097)
                     /locus_tag="ACG8WP_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFP9028073.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(11098..11595)
                     /locus_tag="ACG8WP_001772"
     CDS             complement(11098..11595)
                     /locus_tag="ACG8WP_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFP9028074.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(11906..12583)
                     /locus_tag="ACG8WP_001773"
                     /pseudo
     CDS             complement(11906..12583)
                     /locus_tag="ACG8WP_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(12583..13200)
                     /locus_tag="ACG8WP_001774"
     CDS             complement(12583..13200)
                     /locus_tag="ACG8WP_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028075.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(13214..13384)
                     /locus_tag="ACG8WP_001775"
     CDS             complement(13214..13384)
                     /locus_tag="ACG8WP_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028076.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(13732..15222)
                     /locus_tag="ACG8WP_001776"
     CDS             complement(13732..15222)
                     /locus_tag="ACG8WP_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9028077.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(15265..15564)
                     /locus_tag="ACG8WP_001777"
     CDS             complement(15265..15564)
                     /locus_tag="ACG8WP_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028078.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(15930..16214)
                     /locus_tag="ACG8WP_001778"
     CDS             complement(15930..16214)
                     /locus_tag="ACG8WP_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028079.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(16733..17218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8WP_001779"
     CDS             complement(16733..17218)
                     /gene="vanZ-A"
                     /locus_tag="ACG8WP_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9028080.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(17371..18282)
                     /locus_tag="ACG8WP_001780"
     CDS             complement(17371..18282)
                     /locus_tag="ACG8WP_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9028081.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5310 a         3728 c         2832 g         6631 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcattttct
     2761 aattgaatga ttcgctcaat catttcaaaa aatacgttct catacataga caaaactttt
     2821 ttgtcatagc ctttgtgttc tgtaatgcca tttttagacc aagtacttac tttattactt
     2881 cgcttgataa tatttcggaa aaccaaatta tcctctttat gttctttgta cagttcttcc
     2941 aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accaatcata
     3001 tctagtccag ggttaaactg ttcttgcaaa tcaatcaaat aggaaatata gttttgaatg
     3061 ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa cgtaatcact cgccacgatt
     3121 gcattatttg tataaacgct tggcgttggt acagtatcaa taataataag atcatagtca
     3181 ctttttaaag gtgctaaaag agtagcaagc aatctacttt cattttcaaa cgtccatgag
     3241 cgagttaatt ttggcagtaa catcaaatca aacgtgccag ggatcaagtc taaattatca
     3301 gtcaaatgaa taatagaaga agccaagttt ccatttttca agccttcata aaaattgaca
     3361 cgtggcaatt ctacctcaaa agtttttgct aagtcttttg tcaatgttgc ctgtaagtcc
     3421 ttatcgatca ttaaaacttt taaattcaat ttgtctgtca agtaagcaaa catagtcgat
     3481 aatttggact ttccaacacc acctttaaag taattattca agataataat tgcttcattt
     3541 ttattgttta aaatacgtct taattcttca agtattttta gtttttcctt ctccataact
     3601 acgccccatt ctttttttgt atagtaatat tgtatcatta agaatcacaa atcacaagtg
     3661 atttgtgatt aatcacttgt gatttgtgat aagtgatttg tgattaatat ataaaagccc
     3721 tctttaaagg gcttttatat ttattttgag aaagatataa aatcaatata tcccttttcc
     3781 ccaattttta caacggcatt gtagggcata tagtttttta tatgaaagca tttgttttaa
     3841 tatttgttcc aaagagccat catggctttg acagagctct tcttctatgc aaatgctttt
     3901 aatccgatcc acacgaaaat tcctatattc atttcttagc ctgcaaaacc gattaagtac
     3961 cagttctgtt cataaaaccc cagtgatata ggctctatca ttctgctttc cggttcttgc
     4021 cctcctgatg caggatattg aattgatatt acccttttat tgcagattgc agtctgaatt
     4081 gctgcaataa cgttatccgc caatgtattt ttctgtccag atgtgctgta cacgttaata
     4141 ctgttctcca actcctgtgc atagtttttt tcaccatgct tcaaaataga tttgatttta
     4201 tacatagctg aatcaaaatc ctttataaat gattcatctc catggcaatt taaaaatttc
     4261 cctgctgtaa tcaatgcacc cacttccgcc gctgtaaaca ttaccggcgg aagtaggaag
     4321 ccctctacaa gaaaatatcc ttttccggcc tcagatccaa ttggtattcc ggcttcttca
     4381 agtgtccgga tatctctgta aactgtcctt aagcttatat taaatcgctg tgcaatttct
     4441 ttggcaggaa ttattttttt tgattgcagc tgaataagaa tagaggttac tctgttaatc
     4501 ctgttcaaat tttattcctc ctctgtgact tccataaaaa gtatatccta tgcgttctta
     4561 agctagctgc tgttagtgct tctcattgca ccctggttat aaaagccata ctcacaaacc
     4621 tcttcaccaa aactctttac atctctaata tagaactcta tctagcgaac ttttagaaaa
     4681 gatataaaac atcagagtat ggacagttgc ggatgtactt cagaaaagat tagatgtcta
     4741 aaaagcttgt agttaaagct ttttagacat ctaaatctag gtactaaaac aattcatcca
     4801 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     4861 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     4921 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     4981 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     5041 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     5101 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     5161 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     5221 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     5281 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     5341 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     5401 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     5461 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     5521 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     5581 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     5641 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     5701 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     5761 acaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc
     5821 atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc
     5881 ttgccccctg aaatccttac atacggcgat atcttctata taagcgtacc ggttccaatt
     5941 ttttcgcagt ttaacttttc cgacgcattt atcgtcttgg tagtaaagat atattatctt
     6001 atcagtattg tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc
     6061 ttggtagctt tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt
     6121 ttcatacctc ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgatatc
     6181 tttcaggtgc cctgctttca tttctgtaat cactgttccc gcctctcttc tatatcagcg
     6241 gcatatgtgc tatccaggca gccggtttta ccagtgtatt ttttatacat gtccctggta
     6301 tattttgtta tattcctatc atactccgga taggcataat gaagcctttc cgccacctca
     6361 ccggataccg ccctgaacaa ttgatggcat agaaataatg cttcccatat gttttcatag
     6421 gaatccatcc ggtaggtgga caaaagtttc tcccacaaat cctcggatat atacctttca
     6481 ataaacttat agttcttgcc tacacttaat ttaaagcctg tttcgatgcc gaccttccat
     6541 gatatcattc tcagcagctc atggcgaaca atctgattaa aatgatcaat agcaaataaa
     6601 atttccttac ggcacaatcc tttaataaca taaggtgtta cattccaaaa ttcattgcag
     6661 caatcatcat actcccttgc gcttggcttt cttacatgat aatctatatc agtcggaact
     6721 atgtcccttt taattctaca atctttatca attagaacct ttattaattt atcgcccttt
     6781 aggtaattat ctaactcttc caagggcaat aaggtaagat caattttatt gtaatcatca
     6841 aatagcataa gataggaaaa tcccttttct tcaggtggga ataattccat atcctccggc
     6901 ttttgcatca ttattatatt cccaaattga ttaagccagt catcattaga tataaacggt
     6961 tctatatcac ttacaaaata tgtaatatca taatcctgaa attcatcttt aggtatatta
     7021 atatttgcgc gtgacccctc aagggtcaca attcgaatac gttcatcctg ttctgctaaa
     7081 gaaagtacta aatccatcat ttctttttct gatctcattt acatactcct cgtttatttt
     7141 ttctatatta ttacatcttc ttttttgccg atacaatcag catcattggg cgtcgcattt
     7201 catccgccat ccccggaata tccatcatgt tctctggcgg ctgtggctcc acaatctgat
     7261 ttattataaa actatttgaa agcagtgtat ttagatatgt ggtcagtgtt ctatgatatt
     7321 ttgtaacctt ttcttccaaa aacatagctg tccgtttgcc ctcataataa taattgtcca
     7381 ccgggaaatg cagtatttct cctttttcgt tataatacca gtcttgtgtt ccatgagcag
     7441 taaaaacagg atgttcaact gtaaaaacta aattgccacc agccttcagc atcctatata
     7501 tcttttttat taaattctca tagtctgcta cataatgaaa cgcaagcgaa cttagtatta
     7561 catcaaagct ctcctctggg aaatccacat cttctatggc acagcattca tattcaatct
     7621 gtggaaaatg ggtttttcct tttgctactt cgagcatttt atgagaaata tcaacaccta
     7681 ctacagagga agcaccgttt tccatcgcat atatacagtg ccatccatag ccgcatccta
     7741 aatcaagcac acgcttaccc ttaaaatcag gtagcatctt tttcaaagtc tcccattctc
     7801 ccgcaccagc cagtcctttc tgcgagcgac tcatttgact gtatttttga aaaaatatat
     7861 tatcatcata tttgttttct ttcatctgaa ctctcctcgt ttaaaaagtt atttatctcc
     7921 gatacaattt cattcacttc tttataaagt ttctcggtca tgtcgtagca ttcaaaaaga
     7981 gaaagaccga gtacttcaaa aatatgatta actctttcgg catatttttc aggtttatat
     8041 tcaaaagttt caagcagttt tatagctttc ttttcgttaa tgcaataagc attattacat
     8101 gcaaatagta cttgatttaa gcatgaaata atacgaaaaa catggcctgc aatataatat
     8161 ttatcctctg cccccgcatt tgcttttaca aacattaaag agaattcagc ttcaaatata
     8221 aaaaagttca tcaagctttt ctttagagca gtagggtaaa tttctgcctg cttttttaat
     8281 tcgcataagc tttcgttctt agcatatagt attttactaa tcgctaattc tccacgatac
     8341 atagcactta tataaccatg gggatgcccg gtctgataat tggcagtaac aattccttgc
     8401 tccgtatctt tgattatttg ttccacccgt tttatatcac gtaatatcaa gtcaacatga
     8461 cacccgttta taactaacca tccaccgcca ttaacccaat caccccacgc tccgggaggt
     8521 acaacaaggt tgtttctatt ctcatcatcc aactctgtag caatttgatt aatcgctgtc
     8581 aggtcaaatg agtctgaatt gtaatagatt ccgatatcta tatcagaatt ttctgtatgg
     8641 gtgcctcttg cacgtgagcc ccctaatacg atgccttcta taaaaggcaa agatgataat
     8701 ttctctgtta ctatttgaat aacattacct accatataaa catcctcctt tattcgttag
     8761 atatttttat ttcttctcca tctggtaaaa taaatgcctg cacaaacttg acacctagca
     8821 catcagcaat ggccttcaac tcatccaagg ttactgtttc tcgttgtaat tttttattga
     8881 aattctgtgg agtctggcca atacgtctag caagttcgga aacacttata ttcatttgct
     8941 cacacagttc tttaatcatt tctgccgtag tcatacagca cctccaagtt tacgctaaga
     9001 atattataaa ccatttggtt gataaacaca atagttatta aaatattatt ataatcagca
     9061 agcaaaagaa aacccacaac tctactgtag agtcatgggc tatttacttc tggttttcat
     9121 atttccattt cgatgccaga tttgaaggct atgacgaagt ggccttcata gactgtaacg
     9181 ctctggatta tcttccttac aagcttatca tcatattcta aggtgcggaa tttgttcttg
     9241 cggataaatt caatcagctc attgattcgc tcgttctcgc cactgaggga ggcatcttct
     9301 actaaaagga tctgtcgctt gtctctcagc tcatcaatct catctgctaa gtggtcatag
     9361 tcttgaccct tgttcgccag cttgattagt tctttttgtt tttcctctag tgagttgtta
     9421 atctctgaaa tttggtattc tgtggtttcg ccaatcacag catgaatatt ctcttccagt
     9481 gtctttatca tattgttccc acctgcaagt agcttattaa tcgcagtcat tactgcacca
     9541 taaagttcat cttcttttac ggttcggttc ttacaaactt caggaccttg ctcgattcta
     9601 gtcacgcatc gccagacaaa ttcttttctt ccgtggatat tccaatagac ccgtctataa
     9661 atatcgccac aatctccaca gaaggtgatg gcacttaaag cgtacttgct actgtaaatt
     9721 cttttgttct tgccttctcc tgtgtagata ttgcttctcc gatgaatttc ttcttgcgcc
     9781 tgcaaaaata actctttagg aataatcgct tcatggctat tttcaacata atattgggga
     9841 acatgccctt catttttcac tcgtttcttg gtcagaaaat ctactgtgac agtcttttgc
     9901 agaagggcat ctccgatgta tttttcgttt tgaagtatct tcttaactga ttctggtcgc
     9961 catcttggtt ttcccgctgc tgttaaaata ccatcctttt caagggctcg accaatgccc
    10021 actagactct gaccctcaag gtattctcgg tagatgcgtt tgataatctc agcttcttcg
    10081 gggacaatag ttaaatttcc atcttcatct ttcgtgtagc ccataaatcg cttatggttg
    10141 acctgcacct ttccttgttg gtatcggtac tgtagcccaa gcttaacgtt ttgtgaaagg
    10201 ctctggcttt cctgctgtgc caaagatgcc ataatagtca gcaaaacctc acctttggca
    10261 tccgtggtgt tgatgttctc tttctcaaaa taaactgata tgttcttatc cttgagctgt
    10321 ctaatgtatt gaaggcagtc tagggtgtta cgcgcaaatc ggctgatgga ttttgtaata
    10381 accatgtcga tgttaccgtc catacactct gcaatcatgc gattaaattc gtcacgcttt
    10441 ttagtgttcg taccagagat accatcatct gcaaagatgc cagcaaactc ccattcatta
    10501 tttttcttta taaactctgt atagtgtgag acctgaacct catagctaga attttgttct
    10561 tctgtttctg tagaaacacg gcaataggca gcaacacgaa gtttctttat cttttctttt
    10621 gcggctgtac ttcctactct tttacgagca ggaataacca ttatattttt ctctgtcact
    10681 ttattcctcg ctttctatca gactgtataa gtattctgct cgttcaaaag gatccactgg
    10741 catcttatta tctgcctttt tcattttgaa tcgttctttg ggagggggag aggtgaaagc
    10801 ggcaagctct accactcgtc ctaaatcctt tgcacgttta tctctaactt cttcagcttt
    10861 atcaaatatc tctttatcaa taattgctgg atacgtatca tttccaaggt agttgacgtt
    10921 tttcaaaatg cgccccatca cagaatgtgt cttttcaata cctgcctgtt cgccagccac
    10981 tgtaagggat agtcctgata tgtatttctc aaagaatacc tttacttgac ctgctgcctt
    11041 ttcatcgaca gtaaccactc catcttgaat tttgtatcca tatggaatat atgccattta
    11101 tctcaccacc ttttctttca gggaaagacc gcatttcaaa ttgaatgtca gctcatccct
    11161 ggaatttaca atgatgttca ctacaaattc ttcaaatagt tcttccgtgt agtcaccatt
    11221 aaaattatct gctgacacat aattaatgag gtcctttatc tcgtttgctc gcaaaacccc
    11281 actcgcagag ttcgttacca ggtttgtttt ttcggtagta agatttttaa tctcactatc
    11341 taaaacattt cgttcctgat taaaaagagc aggttctagg aaacctttgg acatcagtgc
    11401 aataagggta ttgcgttctt ccaatagttg ctccattagc ttatcaatag cgtccattct
    11461 ttcacagtcg ctttcttcat cgatttggct aactgacttg aaaagtggtt ctaggattag
    11521 cttatgactg aaagcaagct tattcatcat ggttgtcaat gtggctttga tttccccatc
    11581 tcgcaaaaac aacatggagc aactttcttt atcttcgata ctatctcaac atgtgtcgtg
    11641 gataaggtaa gtatgtaaaa tggtatgtgg aacatagtga tttgctgtaa atgatttgct
    11701 aactacttta gttctttata caaagtataa atcggatatt ttattatttt ttcttttata
    11761 tcttctgaga ttaggtcaaa gtatttgtcc gctaaatcag aaaggggttc ttttttcatc
    11821 acctaatggg tgatgaagca gaatacttta aagactatta aatcaatgtt aggaaagact
    11881 attagtaaaa ctatgaatta ttcaggaaaa aaggttcttt tttaaagttt tctattgctt
    11941 cctggcgttg ataaatgaga tataaagtcg gtgtttgtac acgtcctaat gaaaatgtac
    12001 cttgtacgcc tttttgttgt aaattcaagg tatataaagg gcttgcgttc atgccgatca
    12061 accaatcggc aatttggcgt gtttgcgctt cttgataaaa gggatagtaa ttcattcctg
    12121 gttgcaaatt ttgaaaaccg cttcggatca catctttttc taagctattg atccataatc
    12181 ttttatacgt tttatcttta gaaaaggcat ttgctttatg gataatcgac caggcgatat
    12241 tttcaccttc tctgtcgcta tctgttgcga caataattgt atttgccttt ttgagaagtt
    12301 cagcaacaat tttaaactgc tttcccttat cttttgcaac ttcaaaatcg tatcgatcag
    12361 gaaaaatcgg caaagattca agtttccaat tttgccactt ttcgtcataa tgacctggtt
    12421 ctgctaattc cactaaatgc ccaaaaccaa aggtgataaa cgtttcatct gtaaatagtg
    12481 ggtctttgat ctcaaaataa ccgtcttttt tggtgctttg ttttaaagca cttgcgtagg
    12541 ctaatgcctg gcttggtttt tcagctaaaa taaccgtact cattaactat ccctcttttc
    12601 attgtttttt ctttgatcga ctgtcacgtt atatcttgct cgataccttc taaacgttcg
    12661 gcgattgatt ccagtttgtt cttcaacttc tttatcggat aaaccattca aaaacaaatc
    12721 gaaagcatgc tttaaacgtg gatcattttc tttaaattta tgctgacgac ctttgaattt
    12781 gccttttttc ttagcaattt cgattccttg tgcctgacgt tccttaattt tttttcgttc
    12841 tgattctgct tgatacttgt acaattcaat gacaaggcta ttaatcaaac gccttaaatt
    12901 ttcatcttca ataccattca ttgagggtaa atttaagact tccagggttg cccccttaat
    12961 ttgaatttga ttcatcaatt ctgttaattc tttattattt cgtcctaatc gatctaattc
    13021 agtaacaata acaatatccc cttcacgaat atagttaagc atagcttgta attgtgggcg
    13081 ttcgaccgat tgaccgctta atttgtctga aaagacctta gaaacgccct gtaacgcttg
    13141 taattgccga tctaagttct gttctttgct actgacacgt gcataaccaa ttttagccat
    13201 tttcaaccaa cctctaaaat tctctcggtt gcaataacca atcagcaata tctacttttt
    13261 caatttcaaa ttgcttatca gaaattgtct tttcgtaagc gataaaatct tgcgcatatt
    13321 gttgctcatt aaaaatagcc accacttcgt cattttctaa aactcgataa ataaattttt
    13381 tcattttact cctcctatta tgcccaactt aaatgaccta ttcaccaagt caattatact
    13441 gctaaaatca tattaggaca aataggtata ctctaatgac ctataaaaga tagaaaatta
    13501 aaaaatcaag tgttcgcttc gctctcactg cccctcgacg ttttagtagc ctttccctca
    13561 cttcgttcag tccaagccaa ctaaaagttt tcgggctact ctctccttct ccccctaata
    13621 attaattaaa atcttactct gtatatttct gctaatcatt cgctaaacag caaagaaaaa
    13681 acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt ttcagcctgt
    13741 atcatagcta aacaaatcga gttgtgtgtc cgttttaggg cgttctgcta gcttgtttaa
    13801 agtctcttga atgaatgtat gctctaagtc aaaagaattt gtcagcgcct ttatatagct
    13861 ttctttttct tcttttttta ctttaatgat cgatagcaac aatgatttaa cactagcaag
    13921 ttgaatgcca ccatttcttc ctggtttaat cttaaagaaa atttcctgat tcgccttcag
    13981 taccttcagc aatttatcta atgtccgttc aggaatgcct agcacttctc taatctcttt
    14041 tttggtcgtc actaaataag gcttgtatac atcgcttttt tcgctaatat aagccattaa
    14101 atcttctttc cattctgaca aatgaacacg ttgacgttcg cttctttttt tcttgaattt
    14161 aaaccaccct tgacggacaa ataaatcttt actggttaaa tcacttgata cccaagcttt
    14221 gcaaagaatg gtaatgtatt ccctattagc cccttgatag ttttctgaat aggcacttct
    14281 aacaattttg attacttctt tttcttctaa gggttgatct aatcgattat taaactcaaa
    14341 catattatat tcgcacgttt cgattgaata gcctgaacta aagtaggcta aagagagggt
    14401 aaacatgacg ttattacgcc ctattaaacc cttttctcct gaaaatttcg tttcgtgcaa
    14461 taagagatta aaccagggtt catctacttg ttttttgcct tctgtaccgc ttaaaaccgt
    14521 tagacttgaa cgagtaaagc ccttattatc tgtttgtttg aaagaccaat cttgccattc
    14581 tttgaaagaa taacggtaat taggatcaaa aaattctaca ttgtccgttc ttggtatgcg
    14641 agcaatacca aaatgattac acgttagatc aactggcaaa gactttccaa aatattctcg
    14701 gatattttgc gaaattattt tggctgcttt gacagattta aattctgatt ttgaagtcac
    14761 atagactggc gtttctaaaa caaaatatgc ttgataacct ttatcagatt tgataatcat
    14821 agtaggcata aaacctaaat caatagcggt tgttaaaata tcgcttgctg aaatagtttc
    14881 ttttgccgtg tgaatatcaa aatcaataaa gaaggtattg atttgtctta aattgttttc
    14941 agaatgtcct ttcgtgtatg aacggttttc gtctgcatac gttccataac gataaacgtt
    15001 gggtgtccaa tgtgtaaatg tatcttgatt ttcttgaatc gcttcctcgg aagtcagaac
    15061 aacaccacga ccgccaatca tgcttgattt tgagcgatac gcaaaaatag cccctttgct
    15121 tttacctggc ttggtagtga ttgagcgaat tttactattt ttaaatttgt actttaacaa
    15181 gccgtcatga agcacagttt ctacaacaaa agggatattc attcagctgt tctcctttcc
    15241 tataaatcct ataaaatagg ttgtttaatt aacttggttt gctttttcat tcaactgttt
    15301 caatattgca tgttttgaaa aagatttttt tcctttataa gtcaattttt ttccactaat
    15361 cgaataaatt attttgttat tttctattaa cttatatata taatcttccc cctccgaaga
    15421 aaaatactta tctgattttg tttctaagta gatatttctc ttttctaact ctttcttaaa
    15481 cgtttctagt gtatagatat ttgctaattt tcttatctcc aataaactat tttttatata
    15541 agttttacat tcatcatgat tcatacaaac tccaccttct ataaatgaat acaaaaaaag
    15601 caatcaaacg atttccgatt gattgcttaa caattcttaa attcagtagc ttagatactt
    15661 gaaaactctc tgatttccct atataatgat agtacggtta tataccgtct tcaaacaaag
    15721 ttaattaaat aacttcttac gagggaagag ttcatctgac taactgataa gcgttggttt
    15781 ggcaatctta tcgggctatg catttataaa atgtcgtcaa acattttata aatgtgtcat
    15841 ggctcttttt tcgtttctat tcagttcgtt gtttcgttat atctagtata ccgcttttaa
    15901 aaaaaataag caacgatttc gtgcattatt cacacgaagt cattgctttt ttcttcttcc
    15961 atttctaaat ccaatgttac ttgttctgat tctgtttctg gttctggttc tgttggctca
    16021 tttgggatta aatccactac tagcgttgag ttagttaact ttgcaatttg ttctagtgtt
    16081 tttatggttg gatctgattt tcctgattct attcgtgaat aatttgatct actcatttct
    16141 aattcttggg agaatttttc ttttgatttg gtacctctca tcttttttaa gctttctcta
    16201 aatgctagtt ccaagtttct cccccctctt tttgtgtaat aaaattcacg ctttatttaa
    16261 attatagctt ttaatatctt aataaacaag tgattaatca cttgtgattt gtgattattc
    16321 caaaagtagg taatttcacc aacctttttc aaaagttttt atcgacgatt attggactgg
    16381 atcgtgtttc ctaaagcgat tctaagcgca tgtttaattt taaagtgggt aattctcttt
    16441 aaaacgtcct acaaagattc aaaaaaagca tggctgctag tgggggtacc gtcaggaaaa
    16501 tgcggattta caacgttaag gaaatttatt aaagagacca attcctcagc actaaaattg
    16561 ataattgggc tttctttgtt actatagtgt taggtaagct ttttacttta tcaccggttt
    16621 tcccctcact tcacacctac gggcgagttt ccccacatcc cgttttccat ctaatccaat
    16681 tcattcaata tctttatgtg tatgaaagat cagattgctt gatttagaca tcttacacgt
    16741 aatttattct taaatgggta cggtaaacga gcaataatac gagcaaaagt atacctacaa
    16801 aaataataac tctgtctaat tttttttgat tcatatgctt attgcttaaa ccatataatt
    16861 tcagtccaag aaagcctcca acagtatttg taattacatc tgttatgtct gtcgctccaa
    16921 tagcgaagat aaattgaatt atttcaaaag taagacttaa aaccagtaca aaagcaaact
    16981 taggtaaaaa tccgatttct ttaaaattga cattcaaaag caagccaaat ggaataaaga
    17041 ttataacatt atctatcatc tctctgaaat tcccagtagc agtaaatgga gtcaagttaa
    17101 gacttctttg atgataatta aatactgata aaatattgta ttgtaatttg aataacacta
    17161 accagattag tgtcactaaa tataaagcta gcaatcctct agataatatt tttcccaata
    17221 taatttcctc taattctttt gtttaagttg tatcattagt atagcaatgt cctagttaac
    17281 gtgggtttta aattaaaaag ctaacgttac cccaaaagtg ctagtacaat aatgctgttc
    17341 attaatttcg ttggtttcat tccgccatcc ttacctcctt gaattagtat gtgttgatcc
    17401 gggaaacact gtcacaatta caccgtctat attgtttcct gatatctcat aacgaagatt
    17461 agtcggcaca tgaatggtgg tatttttagt aacaggataa taaaagatct catatttttc
    17521 cccatttaca ctaacagaaa tggttttttc ttcttttagg taatccatat attcctcgag
    17581 aacgaaattc ttttctttca taatcgcact atgtggtaaa ccaacatagc gaatatgcca
    17641 tggttcatat tgaattcctg ttaactctgt tttgtcctct ggataacgta aaatgaaccc
    17701 gtatttccaa gcattttctt ctatccactt tccttcaggg gctcgttcca ttttcgtcaa
    17761 gcttgatcct acatctagtg ataaacctga attatgctca ctataacctg ctggtaaggc
    17821 atactcagcc cccatttctt ggtaaagcac actttgctca tcaaagtctc gatagccact
    17881 attaataata aaatgactaa cgccaccctt tacagcatca ttgaccatct ctgaaaattt
    17941 ttgtgctatt tcttttgaca tataaatatt actatcaagc aacccgtatc catttattaa
    18001 ttcgtcatgt ttagataaat tcacgatatc tgacttcaca ctttcttggc gaacaggata
    18061 tttactattg attaatagca gatttccttg ataaacctgt tcttctgtaa ttgttttctc
    18121 ttgggtattt tcagaagtcc cactattttc taaatgttct ttgggatttt gatcataatt
    18181 ttgaaattcg actttttcct gagaaaacag tgcttcatta acgtagtcat aacctaagta
    18241 tattaagaat aacaataaca ataaaaaaaa caacttcttc attttcagtc tcctccttaa
    18301 ctatatagtt ggaagtatga aatatttcta gaatcttccc cttaaacggc acaatctcgt
    18361 ttgagattgt gccgttttgc cccgtaaatt acagtcaatt atttcgccct actggattta
    18421 ctattatcac caatgtagat gaattttcat aagtgatacc catgctgaat ttaacgaggc
    18481 tgaactgcct gttgacaatg g
//
LOCUS       DAZKAY010000056        18494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000056 DAZKAY010000000
VERSION     DAZKAY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.056"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8WP_001781"
     CDS             99..746
                     /locus_tag="ACG8WP_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9028082.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8WP_001782"
     CDS             1139..3784
                     /locus_tag="ACG8WP_001782"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9028083.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8WP_001783"
     CDS             4094..5407
                     /locus_tag="ACG8WP_001783"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9028084.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8WP_001784"
     CDS             5397..6050
                     /locus_tag="ACG8WP_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9028085.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8WP_001785"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8WP_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9028086.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8WP_001786"
     CDS             6947..7147
                     /locus_tag="ACG8WP_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9028087.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8WP_001787"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8WP_001787"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9028088.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8WP_001788"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8WP_001788"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9028089.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8WP_001789"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8WP_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9028090.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8WP_001790"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8WP_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9028091.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8WP_001791"
     CDS             13269..13466
                     /locus_tag="ACG8WP_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028092.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8WP_001792"
     CDS             13619..14104
                     /locus_tag="ACG8WP_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9028093.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8WP_001793"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8WP_001793"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9028094.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8WP_001794"
     CDS             14981..15508
                     /locus_tag="ACG8WP_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028095.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8WP_001795"
     CDS             15599..16336
                     /locus_tag="ACG8WP_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9028096.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8WP_001796"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8WP_001796"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9028097.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8WP_001797"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8WP_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9028098.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5981 a         3200 c         4022 g         5291 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa tttt
//
LOCUS       DAZKAY010000057        18216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000057 DAZKAY010000000
VERSION     DAZKAY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.057"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..859)
                     /locus_tag="ACG8WP_001798"
     CDS             complement(35..859)
                     /locus_tag="ACG8WP_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9028099.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(832..1512)
                     /locus_tag="ACG8WP_001799"
     CDS             complement(832..1512)
                     /locus_tag="ACG8WP_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9028100.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1535..2473)
                     /locus_tag="ACG8WP_001800"
     CDS             complement(1535..2473)
                     /locus_tag="ACG8WP_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9028101.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACG8WP_001801"
     CDS             complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACG8WP_001801"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9028102.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACG8WP_001802"
     CDS             complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACG8WP_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9028103.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6437..7243
                     /gene="proC"
                     /locus_tag="ACG8WP_001803"
     CDS             6437..7243
                     /gene="proC"
                     /locus_tag="ACG8WP_001803"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9028104.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACG8WP_001804"
     CDS             complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACG8WP_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9028105.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACG8WP_001805"
     CDS             complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACG8WP_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9028106.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10447..11688)
                     /locus_tag="ACG8WP_001806"
     CDS             complement(10447..11688)
                     /locus_tag="ACG8WP_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9028107.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACG8WP_001807"
     CDS             complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACG8WP_001807"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9028108.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12715..13638)
                     /locus_tag="ACG8WP_001808"
     CDS             complement(12715..13638)
                     /locus_tag="ACG8WP_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9028109.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14376..15662)
                     /locus_tag="ACG8WP_001809"
     CDS             complement(14376..15662)
                     /locus_tag="ACG8WP_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9028110.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15655..16923)
                     /locus_tag="ACG8WP_001810"
     CDS             complement(15655..16923)
                     /locus_tag="ACG8WP_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9028111.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17175..17636
                     /gene="mscL"
                     /locus_tag="ACG8WP_001811"
     CDS             17175..17636
                     /gene="mscL"
                     /locus_tag="ACG8WP_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9028112.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17695..18141)
                     /locus_tag="ACG8WP_001812"
     CDS             complement(17695..18141)
                     /locus_tag="ACG8WP_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028113.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5193 a         3982 c         3184 g         5857 t
ORIGIN      
        1 ttttcgtttt tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa
       61 ccaatattcg tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg
      121 ttgctcctgg aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa
      181 atcctacaca aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga
      241 ttgctgcggg aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca
      301 cagcaatagc cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc
      361 catccacgtg ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa
      421 agaacaatat accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa
      481 tggtcacgat cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca
      541 aattcatcaa tactaacgct agcgccaaac caccagccat taaaatggca atggagattt
      601 ctgaataggt tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga
      661 ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta
      721 aagagacatg agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac
      781 ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt
      841 catatgaaag caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc
      901 atactgtttg atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg
      961 cgcactatgt cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt
     1021 tggttcatca agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg
     1081 ctgcttttgt ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac
     1141 agcttctaaa gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg
     1201 gcctcttgga tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt
     1261 gaaagatgca acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt
     1321 ctcagcaatt tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa
     1381 ggttgacttg gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg
     1441 ataagagaca tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc
     1501 gatataacgc accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt
     1561 tcagattttt ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca
     1621 agctttctaa aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag
     1681 ctaccttcga agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa
     1741 attcttttag ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa
     1801 tcgctacttg ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga
     1861 caaatgtgcg gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa
     1921 gctttttgat ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt
     1981 cgcctaattc ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa
     2041 catgtggatc gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg
     2101 cttcttgcga ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc
     2161 tatccactga atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga
     2221 aaacatctgc atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct
     2281 ccgttccagc agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa
     2341 aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct
     2401 tgttcgtatt tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa
     2461 gatatttttt catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa
     2521 cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt
     2581 taagcatgta tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca
     2641 tttcactaca ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct
     2701 attttacttc ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct
     2761 tttaacccat tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt
     2821 ggtccgcttc cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt
     2881 tgatggatga ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg
     2941 atcatctgct gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt
     3001 tggatggctt gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt
     3061 ttgaccaaca caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg
     3121 ccaccgacaa atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa
     3181 ccgatttctg ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt
     3241 agagcagctg cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc
     3301 ttaacaaaga tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct
     3361 tgataaacat tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt
     3421 ttgtcttctg ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc
     3481 atttccaact catgataccc atcttgccgt ctatataata catccagtcc taaatttatc
     3541 ttggctggcg ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca
     3601 agtattctat catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg
     3661 tcatcgtttc aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat
     3721 tcttataatt ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct
     3781 atcagactga gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa
     3841 taacgtcctt cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa
     3901 tagaaaaaat taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa
     3961 tctgtttctc caacattagc taatggagct aagcctagat tggcttccga gtagcccatt
     4021 ttctcgtata caataaacag attggctaac agatagtcag ctaattcctc agattcttta
     4081 ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg
     4141 aagccaacga ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat
     4201 ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa
     4261 acttcattta cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat
     4321 ccttctttca tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt
     4381 gctagatcaa caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc
     4441 atcactacgt agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct
     4501 tgtttcataa actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct
     4561 aaaacaaagc atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct
     4621 tcttggtaat aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct
     4681 ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta
     4741 gtatcttcag ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca
     4801 ataagccctg agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt
     4861 cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag
     4921 ccgaacaagc tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa
     4981 aattcttttc tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc
     5041 caagataacg tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc
     5101 agaaatgcct ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca
     5161 accaatagat tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat
     5221 agccgactga ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca
     5281 aaatagacag cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg
     5341 ttatctaaaa atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc
     5401 ataaaaggta gaacataata aaacaaccga taatataata gccaaactac tgctacatct
     5461 tgccgaacag gcaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca
     5521 cctgggacca tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa
     5581 agcatcgaaa tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc
     5641 gtccattgtc caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag
     5701 aacaatgtct ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg
     5761 ccgaaaagcc agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca
     5821 ataaatgtaa gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat
     5881 acagtggcta aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt
     5941 gctcctacca caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca
     6001 agaagaaact cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc
     6061 acccaatcat acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg
     6121 atcgtcatgc ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta
     6181 gcaatatttg cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt
     6241 aaaatcagac tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt
     6301 tcccattcct aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat
     6361 ttttccactt tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc
     6421 taaaggagag gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat
     6481 tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg
     6541 acattttgaa aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa
     6601 tcaggaagtt gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga
     6661 aaaagtattg gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc
     6721 aggaaaatca ttaacagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg
     6781 tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga
     6841 agctgccacg gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt
     6901 ctatccttta gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc
     6961 attcgtttac atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa
     7021 acagacagcc actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc
     7081 aagtgataaa aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt
     7141 tgcaggtgta gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa
     7201 tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt
     7261 cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt
     7321 attatttagc ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag
     7381 gataatccag ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta
     7441 aatccgagat ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt
     7501 aacttgattc tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga
     7561 tgtagttttc taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg
     7621 cagctacaag gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg
     7681 cgttgattac tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt
     7741 gagatccttc aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt
     7801 ttgctaattg gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct
     7861 cgaccgaatg cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga
     7921 tcttgattag atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt
     7981 attcgcgaat acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg
     8041 ctggatggat tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc
     8101 ggattggatc gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga
     8161 taccagtcag cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt
     8221 tcatttcatc attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg
     8281 cgcagcgttg aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg
     8341 cacgctgttc agattctttt accattagag ttaactcatg attcagctct tcttcagtcg
     8401 atttcatgat gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt
     8461 tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt
     8521 ttgaactaag tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat
     8581 ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta
     8641 acttactttc tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag
     8701 cttctaacaa tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc
     8761 cagcagctga tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa
     8821 accctaaacc aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa
     8881 aatccacctc catactatct ttttcatttt tatcatctat acagcttcta tgataaatta
     8941 ttctaaactc gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta
     9001 ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt
     9061 attcattaaa cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa
     9121 attgagaaac ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt
     9181 acccactcgt ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca
     9241 aggagagtac ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac
     9301 taaaggcaat tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc
     9361 ataagctgct ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata
     9421 gattttagcg ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc
     9481 gcttttatca acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat
     9541 ccctaaacca tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt
     9601 tacgactttg atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc
     9661 acgacgtact tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat
     9721 ttctggattc ccaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat
     9781 tgtttttgtt ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc
     9841 ttgtaacccg acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc
     9901 agcaactgaa tcgactacta cgatatctac agccccactt gatactaaag catcagcgat
     9961 ctctagacct tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc
    10021 taatttttgt gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc
    10081 gccatttttt tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc
    10141 aggtccatat acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc
    10201 taacgcaaga gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt
    10261 cataatagag cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc
    10321 tttacgatca tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc
    10381 attatcctta tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg
    10441 catttatcat tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt
    10501 atatctgata gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt
    10561 agatgaaata gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc
    10621 cactccggtg aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat
    10681 ctgctccaca cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc
    10741 cagaaaacca gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga
    10801 tacaccagca atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc
    10861 tgttacagtc tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc
    10921 atcgccgtat ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa
    10981 aagagcttga ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc
    11041 atttggtttg gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag
    11101 ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt
    11161 ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt
    11221 caactcatta ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt
    11281 ccctaaagca agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg
    11341 ttgcagattg tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt
    11401 gtatccttcc gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt
    11461 caggtcatct tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc
    11521 agctagagca atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata
    11581 aacttcgata cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac
    11641 ttgtcctagt aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc
    11701 ccttatatag tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg
    11761 tgacaaaaat cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct
    11821 cggtcataag tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt
    11881 gactcagagt tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct
    11941 ttgaacacac cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga
    12001 caagcataaa gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg
    12061 ataaacagca agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct
    12121 gcagccatga cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca
    12181 cgacagacaa tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata
    12241 aaagcagtca taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg
    12301 acaaggcctc gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca
    12361 aaaataactg cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt
    12421 ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt
    12481 acagttaatt tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac
    12541 tatcagcgaa gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta
    12601 tttttattga aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac
    12661 gatccttctt ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc
    12721 tgcgtattga agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac
    12781 tggtacggat tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt
    12841 cgcatttgtt gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc
    12901 ttgataaaca taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa
    12961 ttcaagggtt acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc
    13021 ttctcctgta gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt
    13081 agaagactct ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc
    13141 tactttaagc gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt
    13201 catatagccc acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag
    13261 acttgtccaa aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact
    13321 tactgtttca ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa
    13381 aacatcgact aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc
    13441 ccgcacgtaa aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata
    13501 acgtttttga atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg
    13561 tgcttgccgt aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg
    13621 tttgattctc cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca
    13681 aaagtcatgt cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt
    13741 cacaagtcac aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact
    13801 cggagttaaa cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc
    13861 ggtattagct caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata
    13921 ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    13981 tgcggtaata agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct
    14041 tattacttga tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag
    14101 cttcttcggc aataagccga aatatcacaa aaatttgaga agcaattttc gtaaattcct
    14161 tcttatttct tgaagctgac cacttctgtc acaacctctt tgattacttc gtttgatttt
    14221 attttaatat aagttgatta aaagagagca tgatttgttt tattttctca ctaatttttc
    14281 caacacaact aagaatacca taaaagctga ttttggagga ggttcctact acaagtctaa
    14341 aacgaaattt aagacttttc ctcataaata ctggtttatt ttggtctcat gtaaaactcg
    14401 ctgaaggcct cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc
    14461 gaatcgatga tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat
    14521 tgattggcaa tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc
    14581 ttcagcaaat cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt
    14641 tctttgactt tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag
    14701 tgaaattcac gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg
    14761 tacatcgcta gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt
    14821 gctgttttat agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata
    14881 ccaagtacaa atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct
    14941 tttgtgtttt cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct
    15001 tggttttccc gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa
    15061 accatattgc ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt
    15121 ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag
    15181 ttatttagta tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc
    15241 tgaccgatga ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca
    15301 aaatcgatca gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg
    15361 gttttcgtaa aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag
    15421 acatcaccat cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga
    15481 actttcacct tttctttttc accataggga ataaattcgt tatcgatcga accataattc
    15541 gtactgaaca aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa
    15601 ccgtttggca gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc
    15661 atttgcattt cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg
    15721 ttgtatctct ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt
    15781 tgtttccggc agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag
    15841 agaaagcaga tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat
    15901 ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt
    15961 cttttcatcg ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga
    16021 ggcataataa gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa
    16081 accgccaaat aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac
    16141 atttgtctga taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc
    16201 cttcacgata ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga
    16261 ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac
    16321 aaaaatgtcg atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc
    16381 agtgatcttg tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt
    16441 ttcaaaataa agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact
    16501 ttccaaatat agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg
    16561 atttttaata tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag
    16621 gtttggatca ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa
    16681 atttcctttt tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc
    16741 acttaacttt gtctgatccg gataattcaa actatttgtt tccaacatgc tgctgagaag
    16801 tgaacgtttc gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat
    16861 cgttttgtat tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac
    16921 catatttata tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct
    16981 gcctttttta cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc
    17041 taaatgatgt tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc
    17101 taaaagcgta tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga
    17161 agggagggtc attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg
    17221 atcttgctgt cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc
    17281 aaggcttgat cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt
    17341 tagaaggagc actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg
    17401 gtaacgttat tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg
    17461 tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag
    17521 aaactcctac tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg
    17581 cagaagacaa tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa
    17641 gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact
    17701 tgctacttgt tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga
    17761 gattgccttg aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac
    17821 gattggtttt tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct
    17881 tcctaaacca tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc
    17941 cgcttcttca tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt
    18001 atgccaagct tgtccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac
    18061 aaaaactgcg attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa
    18121 tttctttacc tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg
    18181 attcgcacac aaaaaagcaa acgaacctta cgaatc
//
LOCUS       DAZKAY010000058        18072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000058 DAZKAY010000000
VERSION     DAZKAY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.058"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG8WP_001813"
     CDS             133..636
                     /locus_tag="ACG8WP_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9028114.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG8WP_001814"
     CDS             763..1800
                     /locus_tag="ACG8WP_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFP9028115.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG8WP_001815"
     CDS             1815..3512
                     /locus_tag="ACG8WP_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFP9028116.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG8WP_001816"
     CDS             3505..3966
                     /locus_tag="ACG8WP_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP9028117.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG8WP_001817"
     CDS             3979..4839
                     /locus_tag="ACG8WP_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028118.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG8WP_001818"
     CDS             4877..5266
                     /locus_tag="ACG8WP_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9028119.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG8WP_001819"
     CDS             5253..7700
                     /locus_tag="ACG8WP_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028120.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG8WP_001820"
     CDS             7705..9813
                     /locus_tag="ACG8WP_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9028121.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG8WP_001821"
     CDS             9810..10814
                     /locus_tag="ACG8WP_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9028122.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG8WP_001822"
     CDS             10833..11744
                     /locus_tag="ACG8WP_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9028123.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG8WP_001823"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG8WP_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG8WP_001824"
     CDS             complement(12406..12621)
                     /locus_tag="ACG8WP_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028124.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG8WP_001825"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG8WP_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG8WP_001826"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG8WP_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG8WP_001827"
     CDS             complement(15379..16050)
                     /locus_tag="ACG8WP_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9028125.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG8WP_001828"
     CDS             complement(16399..17850)
                     /locus_tag="ACG8WP_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9028126.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5890 a         3029 c         3488 g         5665 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tg
//
LOCUS       DAZKAY010000059        17624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000059 DAZKAY010000000
VERSION     DAZKAY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.059"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG8WP_001829"
     CDS             complement(<1..506)
                     /locus_tag="ACG8WP_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9028127.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="ACG8WP_001830"
     CDS             779..3250
                     /locus_tag="ACG8WP_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9028128.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="ACG8WP_001831"
     CDS             complement(3353..5089)
                     /locus_tag="ACG8WP_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028129.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="ACG8WP_001832"
     CDS             complement(5086..5790)
                     /locus_tag="ACG8WP_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9028130.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="ACG8WP_001833"
     CDS             5921..6424
                     /locus_tag="ACG8WP_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9028131.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="ACG8WP_001834"
     CDS             6384..6722
                     /locus_tag="ACG8WP_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9028132.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8WP_001835"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8WP_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9028133.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="ACG8WP_001836"
     CDS             8273..8851
                     /locus_tag="ACG8WP_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9028134.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="ACG8WP_001837"
     CDS             8855..9376
                     /locus_tag="ACG8WP_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9028135.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="ACG8WP_001838"
     CDS             complement(9455..10009)
                     /locus_tag="ACG8WP_001838"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9028136.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8WP_001839"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8WP_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9028137.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="ACG8WP_001840"
     CDS             10549..10800
                     /locus_tag="ACG8WP_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9028138.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="ACG8WP_001841"
     CDS             10925..11716
                     /locus_tag="ACG8WP_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028139.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8WP_001842"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8WP_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9028140.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8WP_001843"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8WP_001843"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9028141.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8WP_001844"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8WP_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9028142.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13773..14909)
                     /locus_tag="ACG8WP_001845"
     CDS             complement(13773..14909)
                     /locus_tag="ACG8WP_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028143.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15025..15939)
                     /locus_tag="ACG8WP_001846"
     CDS             complement(15025..15939)
                     /locus_tag="ACG8WP_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9028144.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16030..16458)
                     /locus_tag="ACG8WP_001847"
     CDS             complement(16030..16458)
                     /locus_tag="ACG8WP_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9028145.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16463..16837)
                     /locus_tag="ACG8WP_001848"
     CDS             complement(16463..16837)
                     /locus_tag="ACG8WP_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028146.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17123..17308
                     /locus_tag="ACG8WP_001849"
     CDS             17123..17308
                     /locus_tag="ACG8WP_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028147.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17320..17568
                     /locus_tag="ACG8WP_001850"
     CDS             17320..17568
                     /locus_tag="ACG8WP_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028148.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT         5701 a         3162 c         3450 g         5311 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13681 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13741 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13801 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13861 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    13921 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    13981 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14041 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14101 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14161 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14221 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14281 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14341 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14401 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14461 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14521 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14581 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14641 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14701 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14761 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14821 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    14881 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    14941 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15001 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15061 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15121 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15181 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15241 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15301 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15361 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15421 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15481 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15541 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15601 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15661 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15721 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15781 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15841 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    15901 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    15961 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16021 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16081 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16141 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16201 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16261 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16321 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16381 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16441 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16501 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16561 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16621 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16681 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16741 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16801 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16861 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    16921 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    16981 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
    17041 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
    17101 tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc gagcggagat
    17161 gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt caggagctta
    17221 tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac
    17281 agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact aaaaaaacaa
    17341 gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat gtcggaacta
    17401 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
    17461 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
    17521 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
    17581 tttgagaggt gaaggttaat gggtaaattc aacagagcat tggt
//
LOCUS       DAZKAY010000060        17385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000060 DAZKAY010000000
VERSION     DAZKAY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.060"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..571
                     /locus_tag="ACG8WP_001851"
     CDS             <1..571
                     /locus_tag="ACG8WP_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028149.1"
                     /translation="ILFFLCYSYLKTSHAPSSALGWLLLDHSDITEAEENPFFLILSS
                     LCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFIMNGL
                     VVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQVAG
                     NLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(705..830)
                     /locus_tag="ACG8WP_001852"
     CDS             complement(705..830)
                     /locus_tag="ACG8WP_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9028150.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(964..1857)
                     /locus_tag="ACG8WP_001853"
     CDS             complement(964..1857)
                     /locus_tag="ACG8WP_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9028151.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2039..2965)
                     /locus_tag="ACG8WP_001854"
     CDS             complement(2039..2965)
                     /locus_tag="ACG8WP_001854"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9028152.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3050..3811)
                     /gene="pflA"
                     /locus_tag="ACG8WP_001855"
     CDS             complement(3050..3811)
                     /gene="pflA"
                     /locus_tag="ACG8WP_001855"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9028153.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4055..6286)
                     /gene="pflB"
                     /locus_tag="ACG8WP_001856"
     CDS             complement(4055..6286)
                     /gene="pflB"
                     /locus_tag="ACG8WP_001856"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9028154.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6563..9013)
                     /gene="parC"
                     /locus_tag="ACG8WP_001857"
     CDS             complement(6563..9013)
                     /gene="parC"
                     /locus_tag="ACG8WP_001857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9028155.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9025..11070)
                     /gene="parE"
                     /locus_tag="ACG8WP_001858"
     CDS             complement(9025..11070)
                     /gene="parE"
                     /locus_tag="ACG8WP_001858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9028156.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11236..11670)
                     /locus_tag="ACG8WP_001859"
     CDS             complement(11236..11670)
                     /locus_tag="ACG8WP_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9028157.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11797..12435
                     /gene="plsY"
                     /locus_tag="ACG8WP_001860"
     CDS             11797..12435
                     /gene="plsY"
                     /locus_tag="ACG8WP_001860"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9028158.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12567..13442)
                     /locus_tag="ACG8WP_001861"
     CDS             complement(12567..13442)
                     /locus_tag="ACG8WP_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9028159.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13524..14315)
                     /gene="codY"
                     /locus_tag="ACG8WP_001862"
     CDS             complement(13524..14315)
                     /gene="codY"
                     /locus_tag="ACG8WP_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9028160.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14333..15733)
                     /gene="hslU"
                     /locus_tag="ACG8WP_001863"
     CDS             complement(14333..15733)
                     /gene="hslU"
                     /locus_tag="ACG8WP_001863"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9028161.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15747..16295)
                     /gene="hslV"
                     /locus_tag="ACG8WP_001864"
     CDS             complement(15747..16295)
                     /gene="hslV"
                     /locus_tag="ACG8WP_001864"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9028162.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16332..17237)
                     /gene="xerC"
                     /locus_tag="ACG8WP_001865"
     CDS             complement(16332..17237)
                     /gene="xerC"
                     /locus_tag="ACG8WP_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9028163.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         4571 a         3705 c         2962 g         6147 t
ORIGIN      
        1 gatcttattt tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt
       61 aggctggttg cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt
      121 gattttgtct tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca
      181 tcgacatcct ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc
      241 ttttttagca attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa
      301 tggtttagtc gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca
      361 attgaaatgt ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt
      421 ttcctttttt gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg
      481 aaatctgttg gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa
      541 caaacaaaat aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag
      601 ttttaaacac ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc
      661 attcgtttct tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct
      721 tcccataaaa tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt
      781 cttatcacta cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt
      841 ccagaccatg tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt
      901 aaccataaag gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct
      961 ctcttaggtt ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg
     1021 tataaatttc ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat
     1081 tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg
     1141 taattctaat gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg
     1201 gaaaatacta ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc
     1261 tatttgatca aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa
     1321 tgccttcgtt acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg
     1381 gaagtgttct atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct
     1441 cagcaattct gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc
     1501 ttcgaaacaa tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag
     1561 tctcttcaat tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt
     1621 aggatcaaat attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc
     1681 atcgatcaat aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc
     1741 tcttagaatc gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag
     1801 cggatcatcg gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata
     1861 tgttctcttt tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga
     1921 acaagagtca taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg
     1981 tttcttttta tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt
     2041 agctaaatac agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa
     2101 gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt
     2161 taccagcaac aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca
     2221 gatcgtagcc atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat
     2281 ccatcacttc attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca
     2341 ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg
     2401 ttcctgcttt caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa
     2461 gttcttttgc agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat
     2521 ctgaaatgat agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt
     2581 ctttatatag ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca
     2641 atggattagc tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta
     2701 cttcgctgat atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg
     2761 cattttctgc tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg
     2821 cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt
     2881 cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt
     2941 gaccaaaaac taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata
     3001 atagcaaaaa aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta
     3061 aatattttgt atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg
     3121 taggtggttc gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc
     3181 ccatcgtatg ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt
     3241 tgtctagtcg gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa
     3301 tccaaaccgg tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt
     3361 cccaatgcgt tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta
     3421 aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag
     3481 gttttccgca tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt
     3541 cgatcaaaaa atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat
     3601 ctccccagta aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt
     3661 tccctccgcc aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc
     3721 cttgcataaa cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag
     3781 aatgaacata gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat
     3841 tttaagtaca agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg
     3901 cttcgttatg tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt
     3961 tctttcaaat aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc
     4021 gacgctaagt cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca
     4081 tcaagacatc taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata
     4141 cccggatcgt caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat
     4201 tactgaatac attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat
     4261 tacgcaagtt ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt
     4321 gggtatttga aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc
     4381 tagctaataa cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt
     4441 ttccagcttc acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag
     4501 taatcgtcaa taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt
     4561 gtcccatgaa gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc
     4621 cgtatttagg gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga
     4681 ttgcttttac ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag
     4741 caatacctgt agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt
     4801 cataagaata tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg
     4861 ctaaccaatc catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg
     4921 atgtgattgg acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat
     4981 tgatggcata taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt
     5041 ttccgacaac tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt
     5101 cacgaagctg attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag
     5161 catatgttct gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg
     5221 gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg
     5281 aacgaccatc taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga
     5341 ataattggtt atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt
     5401 ggtcgatcaa ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga
     5461 tatagatatc aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt
     5521 tgattgctgc taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg
     5581 gtttagaaat atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat
     5641 attgttcagt gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag
     5701 tcatatcatg atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac
     5761 gacgatagtc accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt
     5821 tgtttttacg agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg
     5881 ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt
     5941 ttagcgcatt gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt
     6001 tgtctgtctg tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg
     6061 aagtaatagt tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga
     6121 tatcatttaa cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca
     6181 aaaagctgtc atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta
     6241 cgccttcttt ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct
     6301 aaatttctgt tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg
     6361 caagggtttt cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc
     6421 aacgttgaca taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat
     6481 aaggatttat taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct
     6541 cttcattaaa atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt
     6601 cgatgacttc tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac
     6661 ctatagggaa atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta
     6721 tttcttttga tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac
     6781 gcatgaccgt cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc
     6841 gtttaacagc tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa
     6901 gcagcccgtt gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct
     6961 ttgctccaac aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta
     7021 ctaagaaaac atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt
     7081 cttccgcctt tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga
     7141 actctgtcat tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct
     7201 ctgcttgaat tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg
     7261 agattgtttg agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga
     7321 cagggcggta gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca
     7381 attcacctga gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag
     7441 cactataaga acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta
     7501 cgtcttcttg tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga
     7561 ttgtcgttag acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa
     7621 cagagaaaag ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca
     7681 agctttcgga ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca
     7741 atgtgacaat tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt
     7801 tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata
     7861 aagctttcat caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt
     7921 gactacgttt tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca
     7981 gtccaacttg atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct
     8041 gtagctcagt gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca
     8101 attcgacgac aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat
     8161 cgattttctt attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt
     8221 catatgggat ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg
     8281 ttttcgaacg aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt
     8341 ttccttgcaa gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt
     8401 caaggctcgc ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt
     8461 tatgagtcgg gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa
     8521 ggtttgggta tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca
     8581 cgagatcgac tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc
     8641 tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt
     8701 gcatttcgat cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat
     8761 aaatactgat gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg
     8821 atttacggaa tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc
     8881 gttgcactgg cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt
     8941 tggaataacg tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt
     9001 gtcgattttc catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt
     9061 atctgctcgt tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc
     9121 ttttttatct aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca
     9181 ttttcttcga ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc
     9241 agcatcatcg atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca
     9301 caattgttcc gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc
     9361 gactttttta atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt
     9421 ctttccttgt cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc
     9481 ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc
     9541 gccgtctgta tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga
     9601 aaactcagga ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt
     9661 caagatatct tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat
     9721 tgcttggaac tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac
     9781 taaatacaat tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag
     9841 aagagattcg ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc
     9901 tgcttctctc gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct
     9961 attttcctgc aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc
    10021 aactggcgta ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac
    10081 acggatagaa agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc
    10141 ccgttctttc agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc
    10201 tttcatacca gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa
    10261 aacattttct gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt
    10321 ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac
    10381 aaattctttg attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag
    10441 atcagaaaga gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc
    10501 taaaatttca tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac
    10561 agtcgtcccg tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc
    10621 gccgttttta aatttctgct gatactctac gccatctcta acgatcgtta caattagcca
    10681 tttcgataag gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta
    10741 ccctccttga ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg
    10801 tattccggaa gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact
    10861 attgtcttca tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac
    10921 tgcattatcg acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat
    10981 gtacatccct ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc
    11041 attgtattcg ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg
    11101 caaaaccgca ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag
    11161 aaatcaattt taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct
    11221 tctttgtggt catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca
    11281 acgatccatt acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt
    11341 ttcaatccct aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat
    11401 ttcaggtaaa aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc
    11461 tgtcaaattc gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac
    11521 tggtatgatt ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc
    11581 tggtttattg ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat
    11641 cgtttcttgt gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc
    11701 catctattat acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa
    11761 ataaaggata gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt
    11821 ttagcatatc ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa
    11881 aaagatatcc gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta
    11941 ggcaaaacag ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact
    12001 tgtttgccga tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc
    12061 attcttggcc atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca
    12121 agtgcaggaa tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt
    12181 atcgttagtt tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg
    12241 atcactttat ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat
    12301 tggttactga cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac
    12361 aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc
    12421 aagaaaaaat cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga
    12481 tgtcacactc ccttattact tacaggatag cttttttaca caccttggta aacggtgttc
    12541 cctgctaact tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag
    12601 atttcatggg cacctagttg attgatccct actttttcca tcaagtttcc actactgttc
    12661 accgtgtcag cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa
    12721 ggagaccaga tccctacata aggcatatct ttatagctta aagttacgct atgggaagat
    12781 ttttcacttc gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc
    12841 tcaaaaagtt cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc
    12901 atatcgatga atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc
    12961 agatagaaat cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca
    13021 ccgatagaaa agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg
    13081 ccgtctcctc ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat
    13141 gtctccggcg tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca
    13201 aaatccatat cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca
    13261 tactgatcat cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa
    13321 tacgctggat caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc
    13381 aattccgcac cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc
    13441 attgccattc ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc
    13501 tatctgcccc agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg
    13561 gaattcaata cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca
    13621 gctgattcca attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg
    13681 gcaatattag aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt
    13741 acagccttca attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc
    13801 cgtacatctg cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta
    13861 gctccatatt ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag
    13921 ataatcgtac ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat
    13981 ggatatttct ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta
    14041 gcttcagttt tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca
    14101 acaaacattg ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat
    14161 actccgtcac ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc
    14221 attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg
    14281 tttatcttgc gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa
    14341 tatagcgact taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt
    14401 ctgtgatcgt tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta
    14461 gtttttccaa aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat
    14521 cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt
    14581 ctgtaccgat caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct
    14641 tcacaaaatc ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa
    14701 gttctggaat tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga
    14761 cgtgatctgt ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca
    14821 atatgtctct ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga
    14881 tcttatcgat ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac
    14941 tatggatatc agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag
    15001 cttctttgac tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt
    15061 catttaggtc gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt
    15121 ggtcttcaac ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca
    15181 agactgtacg gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat
    15241 tttgctgact agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct
    15301 gttctttttt gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag
    15361 cacttgtgta tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta
    15421 aatcccgaac cattgattct acatctcttc cgacataacc gacttcagta aattttgttg
    15481 cttcgacttt gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt
    15541 taccgacccc tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt
    15601 tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg
    15661 ctgcgtgctg tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt
    15721 ttaattcata catatctact cacctcttac agttcttcta cgataatatt atgattagta
    15781 aagatacaga tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg
    15841 tcgttttgag catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat
    15901 ccgatagcta agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc
    15961 atttctttat cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc
    16021 cgccattctt gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt
    16081 ttgccttcaa atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca
    16141 acaacttctc cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat
    16201 tcacccattg ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca
    16261 cagatagtgg ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct
    16321 tttccttatt tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt
    16381 gttacatggg catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact
    16441 gtccgcaaat ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga
    16501 ggatggatat cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt
    16561 ccagtaggcg tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga
    16621 tattttgtca tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca
    16681 aaagaaccta gaggagcata tctctctttg ttccctttac catggatcaa catgacctct
    16741 gaatcaaaat caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac
    16801 ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg
    16861 ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc
    16921 tttttgagat gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac
    16981 tgataaaacg aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga
    17041 cgatgatcag ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga
    17101 tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct
    17161 tctttcgttt tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc
    17221 cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg
    17281 tttataaaaa gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat
    17341 tggcgcaatt cctcacattt tgtttgagaa attcttaaga aaaac
//
LOCUS       DAZKAY010000061        17005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000061 DAZKAY010000000
VERSION     DAZKAY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.061"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..124)
                     /locus_tag="ACG8WP_001866"
     CDS             complement(<1..124)
                     /locus_tag="ACG8WP_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFP9028164.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="ACG8WP_001867"
     CDS             complement(228..539)
                     /locus_tag="ACG8WP_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9028165.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="ACG8WP_001868"
     CDS             complement(541..738)
                     /locus_tag="ACG8WP_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9028166.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="ACG8WP_001869"
     CDS             complement(1132..2859)
                     /locus_tag="ACG8WP_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9028167.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="ACG8WP_001870"
     CDS             complement(2852..4045)
                     /locus_tag="ACG8WP_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9028168.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="ACG8WP_001871"
     CDS             complement(4232..4876)
                     /locus_tag="ACG8WP_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028169.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="ACG8WP_001872"
     CDS             complement(4970..5104)
                     /locus_tag="ACG8WP_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9028170.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="ACG8WP_001873"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="ACG8WP_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9028171.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="ACG8WP_001874"
     CDS             complement(7235..7708)
                     /locus_tag="ACG8WP_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9028172.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="ACG8WP_001875"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="ACG8WP_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9028173.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="ACG8WP_001876"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="ACG8WP_001876"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9028174.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="ACG8WP_001877"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="ACG8WP_001877"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9028175.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="ACG8WP_001878"
     CDS             complement(11755..12894)
                     /locus_tag="ACG8WP_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9028176.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="ACG8WP_001879"
     CDS             complement(13203..13916)
                     /locus_tag="ACG8WP_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9028177.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="ACG8WP_001880"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="ACG8WP_001880"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9028178.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="ACG8WP_001881"
     CDS             15105..16637
                     /locus_tag="ACG8WP_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9028179.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="ACG8WP_001882"
     CDS             complement(16828..>17005)
                     /locus_tag="ACG8WP_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9028180.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DAZKAY010000062        16178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000062 DAZKAY010000000
VERSION     DAZKAY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.062"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /gene="scm"
                     /locus_tag="ACG8WP_001883"
     CDS             <1..435
                     /gene="scm"
                     /locus_tag="ACG8WP_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9028181.1"
                     /translation="STTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDS
                     TDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNG
                     NKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(590..1576)
                     /locus_tag="ACG8WP_001884"
     CDS             complement(590..1576)
                     /locus_tag="ACG8WP_001884"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9028182.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1599..2165)
                     /locus_tag="ACG8WP_001885"
     CDS             complement(1599..2165)
                     /locus_tag="ACG8WP_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9028183.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2179..2595)
                     /locus_tag="ACG8WP_001886"
     CDS             complement(2179..2595)
                     /locus_tag="ACG8WP_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9028184.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3052..4986)
                     /locus_tag="ACG8WP_001887"
     CDS             complement(3052..4986)
                     /locus_tag="ACG8WP_001887"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9028185.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5288..6223
                     /gene="pplA"
                     /locus_tag="ACG8WP_001888"
     CDS             5288..6223
                     /gene="pplA"
                     /locus_tag="ACG8WP_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9028186.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6313..7416
                     /locus_tag="ACG8WP_001889"
     CDS             6313..7416
                     /locus_tag="ACG8WP_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9028187.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7429..8382
                     /gene="menA"
                     /locus_tag="ACG8WP_001890"
     CDS             7429..8382
                     /gene="menA"
                     /locus_tag="ACG8WP_001890"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9028188.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9196..10542)
                     /gene="gor"
                     /locus_tag="ACG8WP_001891"
     CDS             complement(9196..10542)
                     /gene="gor"
                     /locus_tag="ACG8WP_001891"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9028189.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10676..11929)
                     /locus_tag="ACG8WP_001892"
     CDS             complement(10676..11929)
                     /locus_tag="ACG8WP_001892"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9028190.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11935..12858)
                     /locus_tag="ACG8WP_001893"
     CDS             complement(11935..12858)
                     /locus_tag="ACG8WP_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9028191.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13079..15571)
                     /locus_tag="ACG8WP_001894"
     CDS             complement(13079..15571)
                     /locus_tag="ACG8WP_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9028192.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15578..16045)
                     /locus_tag="ACG8WP_001895"
     CDS             complement(15578..16045)
                     /locus_tag="ACG8WP_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9028193.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4752 a         3381 c         2875 g         5170 t
ORIGIN      
        1 tctaccacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tactacctct
       61 accacaagtg atagctcaga ttcatctacc actacaagtg atagcacgga cacgtcagct
      121 tcaagtgaca gtacagatac atcctctacc acaagtgata gctcagattc atcaacttca
      181 agcgatagta cagacacgtc gtctaccaca agcgatacac gtcatgcaag tgtctccttc
      241 tcaaaaacaa gttcggataa caggacgaac tttaacttaa ctaataaaac aagcaatgac
      301 aatggaaaca aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa taattggata
      361 acactcgcag gcgttatatt acttgtaatc ggtttaagaa tcacttttat gagttataag
      421 aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta acggacgttg
      481 ctctttattt ttgcttttta tcataaatac aagaactaca attcaatttg tcaaaaaaca
      541 cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt cagtctttgc
      601 gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga gaggtggcag
      661 gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt
      721 cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct tcttctgtca
      781 ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc aaagagtaga
      841 tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg ccgatttcct
      901 ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc ccgatagaaa
      961 gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca aaagcgccaa
     1021 cagaacaact taattggaac agttcagctg tcttacctga aatattttcc aaatattctt
     1081 ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca ttcaatactt
     1141 tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa tcagacaata
     1201 gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca ttaccaaact
     1261 gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata tcatcatgga
     1321 tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct ttgtctcgat
     1381 cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac agctttccgc
     1441 cggcatggat cgtttctttg attgcttgtt cgacaggttt gtttttcagg cggacagttt
     1501 cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag ttttcccaca
     1561 ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt aaacattggg
     1621 ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag tgtctgcaca
     1681 tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat ccctaaaact
     1741 gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga agccatcaaa
     1801 agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat actgacacgt
     1861 tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc accactcata
     1921 ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag tcgtagccaa
     1981 agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt gattagattg
     2041 gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat attttccaat
     2101 aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt ttgtaattta
     2161 ctcatggtgt gtaaaggtct aataaatcag actgtcttcg tcacctccat ctgttgaacg
     2221 gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt tcgttgccca
     2281 gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta ctcgatcgcc
     2341 atctatctcg atcaagttat aatctccgtg ttcatctttg taagtatatg tgttgctctc
     2401 ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca gtaccgcttc
     2461 tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta aaaaagaaag
     2521 aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt tttcagcaaa
     2581 ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat tttagcataa
     2641 aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac
     2701 agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg tggaacaccg
     2761 tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag gaatttacga
     2821 aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc tacttctgga
     2881 acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag
     2941 gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt gacggtttgt
     3001 ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta tttatttctt
     3061 gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt gtagccatgg
     3121 aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac
     3181 aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg tgatcaccaa
     3241 tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg ctaatgccaa
     3301 ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat tgtttggcat
     3361 gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag ccattggttc
     3421 ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt ctccggcact
     3481 tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac cactggcaac
     3541 atctgttaca ttgtattccc atgtccaagg gaacacactg tttccagtga accatgatcc
     3601 ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt ttttggaagc
     3661 ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata agacattgcc
     3721 gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga aaaactcatg
     3781 cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt tcaaattcac
     3841 gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc
     3901 taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt taaatttatg
     3961 cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc
     4021 agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa agactaaatc
     4081 accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt cattggcgat
     4141 caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag ttcctttgac
     4201 accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg
     4261 atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt gcttacgcaa
     4321 atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg tcggcattgc
     4381 ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg ctaggcgatt
     4441 tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac cacagacaac
     4501 gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt ttgctactgt
     4561 cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg tgaatccgtg
     4621 tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa ggatcagctg
     4681 atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat cgataccgat
     4741 tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct gcagatcgta
     4801 ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg tcatcatagt
     4861 atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca gtttctttgc
     4921 caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa tattttgctt
     4981 tgccattgat catccgctcc ttatagacta tctatataat gaatatatga taattctttt
     5041 agtttgatac ttattacaca atctccatat attatataga tgttctgttc ttttgtctaa
     5101 ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa
     5161 gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta taggctaaaa
     5221 agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg
     5281 tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat tttcagctct
     5341 agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt ctgaatcatc
     5401 tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag ttgtagctgg
     5461 cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa atggttaccg
     5521 tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat atgataacgt
     5581 caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa
     5641 atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct taaaagcaca
     5701 aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa gcttccaaaa
     5761 ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa tcgaaatcga
     5821 caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact actctaacgg
     5881 ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat caaactttga
     5941 ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg aaaatatgaa
     6001 agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg aattcgtcaa
     6061 agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag gtgctacaca
     6121 cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg aaaaaggcga
     6181 cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata atcatactaa
     6241 taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa
     6301 gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg catctctttt
     6361 atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag ttcagcagct
     6421 accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac ttacgttcgt
     6481 atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga tcgagtaaaa
     6541 gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga agtgaatgag
     6601 caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt caagcgggcc
     6661 tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa
     6721 ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat cgatcaggca
     6781 ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta
     6841 aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat tacggacgaa
     6901 gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt gggcggtaat
     6961 gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt cggcatccaa
     7021 gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa caaaacatta
     7081 gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata ccatcatttg
     7141 tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac gatcataaca
     7201 gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa
     7261 ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa
     7321 gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc ggaggactcc
     7381 cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat gacactaaaa
     7441 atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc ttttgcggta
     7501 ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac ttttttattt
     7561 tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta catggattat
     7621 gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg cagaaatcta
     7681 tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt tgttatcggc
     7741 gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat ctgttgtttt
     7801 attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt aggggaaatt
     7861 ttcagtgggg tgactatggg ccttggaatt tttaccatgg tcgtttatgt caatacattc
     7921 ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttgc tttggtagga
     7981 aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt tacgattgcc
     8041 aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa tcatcgctat
     8101 acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct attgatgtat
     8161 gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg gccgatcttg
     8221 gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa gcaaacgtta
     8281 ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa tgccagttat
     8341 gtattggggc tattactaac aatcattatg caagccattt aacagcatga agaggttgtg
     8401 aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc cttatatttt
     8461 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta tcaggaggtt
     8521 gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     8581 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt
     8641 tgtaaaatca gtgttttgtc ctgagtatta gagaaacaaa tggaaaaata gttcctcata
     8701 ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacactatt tgttcggaaa
     8761 aagtgactaa ctatgacaca atttttttca cagtcccttt tttcacttga tttgattcgt
     8821 accagcgaac ttgacgagaa ctttcttggt ttggtaacta tatggtattc ttttaacatc
     8881 ctcgtttgct tcttctaatt tatttataca aagtcacact tttttaaaca tctgttcttc
     8941 gatacatttc tatttctttt aaacatcgat accaatattt tgaacttaag agtgcatacc
     9001 aaatggattc atttactctt cgtcagttac gaagataatc aaaatctcca acttccctac
     9061 ttatttcgtt ttgagaatta tcgaagcaat cttacataaa taactaaaaa acaaaaaacc
     9121 agaaaatcag tatactgatt ttctggtttt acatggtttg ttcaataaaa agctgttgga
     9181 actgttaata ttaaattatc gcatcgttac gaattcttcc gctcctgttg gatggatggc
     9241 tactgtattg tcaaagtctt ctttagttgc acccatcttg atagccacgg caaaaccttg
     9301 cagcatctca tcgacaccaa caccaatagc atgtaagccg acgattcttt cttcttttcc
     9361 gacacagatc aatttcattt cgcatttttg gcgataatca tttaatgcaa agtacattgg
     9421 ggtaaagcgt gaacgataaa ctttcaattc attttcgcca tatttttcca atgcttcttc
     9481 ttctgtcaaa ccaattgttg caattggtgg atgcgtaaag acgactgtcg gaacaagttt
     9541 atagtcaaga taagaatctt tctttccatt aaataaacgt tctgataaac gtctaccagc
     9601 tgcgattgca acaggagtca gatctaattt accgatcaca tcaccaacag caaaaatatg
     9661 ttcatccgtt gtattttggt atttatctac tttgataaac ccttttttgt ccaattccac
     9721 atttgtattt tccagaccta atttttcggt attcggtaca cgtccaccgg caaaaagtac
     9781 acaatcacca gttaaggttt caccattttc aaatagcacc gtatattcgt cattgttttt
     9841 agtaatttct ttagcaatat gatgtgcgta agtttttatt ccagcttctt ggtacatttg
     9901 gatcaaattg tccgaaagca ttttatcaaa cgttctaagt ggtcgttctt ttcgatatgc
     9961 ccacatgact tcactaccta aaccacgaaa tacacctgaa agttcagccg caatgtatcc
    10021 agcacctaaa acgattgctc tcttaggtaa ctctgttaat tcaaaaaatc catttgaatc
    10081 aattgcgtat tctccaccag gaatggtcat tttacttgca cggcctccag tagcgatcaa
    10141 gattttagga gcacgatagg tttcaccgtt tacttcgatc gtttgactat cgataaaagt
    10201 agcgtaacct tttatagcct cgacatgatt gctatctaat cctctttgat aagcaccgtg
    10261 tagaaaatca atatattttt ctcgtctttc tactagaccg gcgaaatcaa cattttttaa
    10321 atctgcttgg ataccgtaac ttgaggcatc tcgttcaatt gtttccaaaa tcgtacttgc
    10381 ttgccacata acttttttag gtacacaacc gacgttgaca catgttccgc ccaattcatt
    10441 cgcttcaatc agcaaaactt ttgcgccatg catccctgct ctgtttgctg aagcaatacc
    10501 gccagagccg ccaccaatta cgatgtaatc atatatttgc attttgttcc tccttattga
    10561 agagatcaat caaaaccttt atttaacgat cctttttcta cagaaaacaa gtgtaacact
    10621 tttcttgaat tttgtatgcg aagatgctta aaacagggat atatcagctt tttccttatt
    10681 tacgttttcg gatcatatct ccaggtttca ccggctgtgc gactggcata gttaaaatca
    10741 tcattggatt tggtgctcgg tcaatggatt cgccttcttc attatgcata acttcaacgg
    10801 tttgactgaa atgactgaat cctggaccat aaaattcgat ttcatctcct acagaaaaca
    10861 agtttctctg acgtatcgta gcaatctgtg ttgcctcatc ataagataat acttcaccga
    10921 caaatttata tactgggatt ttacgacgtt caccgaataa ttgttcgttt tcagttggtg
    10981 tattgtagta aaaacctgta gctaattctc gttgtgctac tttccataac tcgtcgatcc
    11041 attcttgctt gcagacatag ttttcaggat cttccatata actgtctaca gccgctttat
    11101 agacatttga caccgtagat acataatgga tcgatttcat tcttccttcg atcttgaagc
    11161 tgtctacgcc gttttcaatc aattctggaa tatgttcgat cattgacata tccactgcac
    11221 tcatcgagaa ttcttcttcc acttcgcctg tatcagttaa actgtttcgt tcttgaccaa
    11281 aaggcatatc gaacaatcca tacttccagc gacaagattg agaacatccg ccacggtttg
    11341 catcccgcat cgacatatgg ttggacaatg tgcatcgacc agaataagag atacacatgg
    11401 caccatggat aaatgcttcg atttcggtat ccgtattttt acgaatctct gccacttcct
    11461 ccattgaaac ttcacgagct aaaacgacac gttccaaccc ttcttctttc cagaattcca
    11521 atgtctcaaa attagttgct gaagcttgag tggataaatg gatcggcaga cctggggctt
    11581 ctgtcgcaca aatttcgatc aaagctggat cagaaacaat cactgcagaa ataccgacat
    11641 cacgcaaact gcggaaaaat tcccctgctc catcttcatt tccttcatgg gtgaccatat
    11701 tagcagcaac gtataccttc gcatcatgtt cttttgcaaa agcaactcct tcagccattt
    11761 cttcataagt aaaattacct gcacggcttc ttaaaccata tgcattcccg ccaatatata
    11821 ctgcatctgc tccataatgg atcgcggttt ttaacttttc cagtgttcct gctggcgcta
    11881 ggatttctgg tcgtttcaat tttcttttta ctgtcatttc tttcatcata ctccttattt
    11941 gatagcagaa ggatcaatat aaaagaatcc tgtatctaat tcacggtttt caggatgcaa
    12001 tttactgatt tcgctagtca ttttttccgc tttttcttga tcccagctac cttctaaaat
    12061 ttgttttttt gcttcggcga atagtcgagt gatgtttacg aatttttcgc ctggagtaaa
    12121 taatccgtcc aatttccatg tacagtaatg atgctgataa agttcattca attcattcga
    12181 gagattcaga tcattgctgg caaaaatatg tgtgccatga ctatcttcat aaatagaata
    12241 atgtgtctct tcttttttag gttccgagat aaaaagacct ctttcacgat ctttttgttc
    12301 gtcttgtttc gtatagttat agtaattctg tagcagtgga cgtttggatt gatggatgca
    12361 tgtagcacca tacacaaggg tttctacagg aatatcgagt ttttcttcca tcgccttcat
    12421 ctcttcaaaa ggaacttcac gagcaagtac tgctccaact gcgccttttt tcgcccagaa
    12481 attgatttga cgtgcgctcg ttactaatgt ttcagcatca taaatatacg gtatacgcaa
    12541 ttcaggattt tttttcatcg tataaataat acctgtatca ccaactgtga tcacatctac
    12601 gtttatttct tttaaaaaag ccaaatattc aggtacattt ttcatttttt caggatgaat
    12661 gatcccattt acagcaatat tcgctttttt accatacttg tgtgctaatt ctaccaattg
    12721 tcgctgttct tctcgtgtaa atgaagcagg cagacgcaat ccaaattgct cttcaccgaa
    12781 atatagtgta tctgcacctg cagcaagcaa ctgttctgct tgttcaatag tttctactgt
    12841 ggttatgagt tcgatcataa ttttctcctc ctacaacttc ataatagtag catagctcta
    12901 aaaactttcc aatacttctt ctatttaaag tcggattttt gaaaattatt tcacaaaaaa
    12961 tcaaaataaa gttcgatata ttctgtccaa atataaaaag agattgtgac aaaaatcttg
    13021 tcacaacctc ttaatcaagt cttactatta taaaaattca gctacacagg ccaagtaact
    13081 atagggaacc tactgtttcc tttttaggtc cgcgaacatt caatgtgatc ttaccttttg
    13141 aagcacctac tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa gcctcgctca
    13201 atcgatcttc aatttctttt tgaagtgcgc gacggattgg acgtgcacca tattcaggat
    13261 caaatccagc tttcccgatc acatcgattg cagatggtgt gattttcact tgaatatctt
    13321 gttctttcaa tcgtttgata atcgctttgc tcatgatttg gacgattgta tggatttcat
    13381 ctttactcaa tgaatggaag acgacagtct catcgattcg attcaagaat tcaggtcgga
    13441 aagctttctt taactcttct aagatacgtt tttgcatggc tttgtgatct ttagtaacat
    13501 cttggacatt gaatccgaca tttttttctt ctcgaatttg tgtcgctccg atatttgaag
    13561 tcataatcat gattgtattt ctaaagtcca cttttctacc tttagaatct gttaaatgac
    13621 catcatccaa tacttgcaga agtaaattga aaacgtctgg atgtgctttc tccacttcat
    13681 ctaagagaat cactgaatat ggtttttgac gaattttttc tgtcaactgt ccaccttcat
    13741 cataccctac gtatcctgga ggagacccga ttaatcggct tgtactgtat ttttccataa
    13801 attctgacat atcaacacgg atcagggcat cttcacttcc aaacatgact tctgaaagtg
    13861 ctttggccaa ttctgttttt ccgacaccag ttggtccaag gaacatgaat gaaccaattg
    13921 gacgatcagg atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa actgctttga
    13981 cagcctcatc ttgaccaacg acacgttcat gcagaagtcc ttccagttct aacaagcgtt
    14041 cgctttcttt tttctccaac tgctgtagag ggacaccagt ccactccgat acaactgtag
    14101 caacatcttc ttctgttaca cgatcagaat aaccttgctc ctgttttgct tcaattgcag
    14161 aaactttttg cagcttttta cgtagtgctt tttcttgtct gcgtaattga gcagcatttt
    14221 caaagtcttg tttttggatc gctgcttcct tttcttgtac tagttcatcg atttctagtt
    14281 tgatcacagt tgattttgtt aagtgatcgg tttgatccaa acggactttc gctgctgatt
    14341 catcgatcaa atcaattgct ttatcgggta gttgacgaga agtgatataa cgaacagaaa
    14401 gattgactgc tgctcgtaca gcttcatctg tgatctccac tccatggtgc tcttcatatc
    14461 ttgtacgtaa tccttgaaga atcacttctg cttcttcagg agttggctca tctacttgaa
    14521 tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat atatttttga tattcatcta
    14581 gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc aagatattcg
    14641 aagcatcaat tgctccttct gctccacctg caccaattaa ggtatgcaat tcatcaatga
    14701 ataagattac ttgaccatca ttatagattt cgtcgatgac ctttttcatt cgatcttcaa
    14761 attcaccgcg atatttcgta ccagcaacca aagatcccat atcaagcatc atcaggcgct
    14821 tttcttgcat gtcttctggc acttcaccta aaatgatttt ttgagctaac ccttcagcaa
    14881 tggctgtttt gcctacacca ggttcaccca ccagtacagg gttattcttt gtccgtcggc
    14941 taagaatctg gataagccgt ttcacttctc tcgaacgacc aacaactggg tccagcttgt
    15001 tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt gttccttgcg
    15061 gtgtttgccg tttatcttgt acatttacat ttctacgacg attcattcct tttccagcag
    15121 aatcaccagc tagccccatt ttttgtttca gaacttgacg ggcttttgaa aggctgatac
    15181 ctaagttgat catgattcga gtagccatga tactttcatc ttttaataag ctcatcaaaa
    15241 tatgttctgt tccaactttt tgagaaccag ttttcttcac ttcgctgcta gctaaagcaa
    15301 aaacttgttt cattcttggt gaatatggaa gaaagactcc tttagggtaa gaacggatac
    15361 caccataacc aatcagatgt tcaatttctt cataaatttc cgtttcattc aaatccattt
    15421 cacgtaatac ttttccagca attccatttt gctcgaccac taaggctagt aaaacatgtt
    15481 ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt tctaatactg
    15541 cttgggctct ttcagtaaaa agttcttcca tctaatctca cttcccatct tcataactta
    15601 aacgttccag aaatgctcgg agaatccgag cacgtgtatg atcttcatat tcattgaaat
    15661 tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt gtaattattt
    15721 ggtcttcata aagtttttga ataatagcaa aagcattttt ctcactgatt gtatcatcaa
    15781 ataactgatt tatctgctca agtaattgtt ttttatctga aatccttact tttgcaattc
    15841 gtatataacc accaccacca cgtttgcttt ctactagata accacgttgg atggtaaaac
    15901 gggtattaat cacatagttg atttgtgaag gaacacagtt aaagaggtct gccatttcaa
    15961 tccttcgaat ctcgatcatt tcactatttt ccagaatttt ctttatatat gattcaatta
    16021 aatccgaggt attctgatgt gccatcttat tcattccctt tgactaatat tgaccttaat
    16081 tataacgaat ttgataatag aaagaaagaa gaacgctttt tgttaaataa atcctttaga
    16141 atcaacaaaa aaaaatacgg aatatactat tccgtatc
//
LOCUS       DAZKAY010000063        15982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000063 DAZKAY010000000
VERSION     DAZKAY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.063"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8WP_001896"
     CDS             226..894
                     /locus_tag="ACG8WP_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9028194.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8WP_001897"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8WP_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028195.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8WP_001898"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8WP_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9028196.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8WP_001899"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8WP_001899"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9028197.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8WP_001900"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8WP_001900"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9028198.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACG8WP_001901"
     CDS             complement(5260..6684)
                     /locus_tag="ACG8WP_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9028199.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACG8WP_001902"
     CDS             6859..7764
                     /locus_tag="ACG8WP_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9028200.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8WP_001903"
     CDS             7855..8532
                     /locus_tag="ACG8WP_001903"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9028201.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8WP_001904"
     CDS             8529..8699
                     /locus_tag="ACG8WP_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028202.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8WP_001905"
     CDS             8813..9709
                     /locus_tag="ACG8WP_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9028203.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8WP_001906"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8WP_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9028204.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8WP_001907"
     CDS             11372..12118
                     /locus_tag="ACG8WP_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9028205.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8WP_001908"
     CDS             12115..12954
                     /locus_tag="ACG8WP_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9028206.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACG8WP_001909"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACG8WP_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACG8WP_001910"
     CDS             14045..14797
                     /locus_tag="ACG8WP_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028207.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACG8WP_001911"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACG8WP_001911"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9028208.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5188 a         2995 c         3221 g         4578 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac at
//
LOCUS       DAZKAY010000064        15139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000064 DAZKAY010000000
VERSION     DAZKAY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.064"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8WP_001912"
     CDS             4..2376
                     /locus_tag="ACG8WP_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9028209.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8WP_001913"
     CDS             2437..3897
                     /locus_tag="ACG8WP_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9028210.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8WP_001914"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8WP_001914"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9028211.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG8WP_001915"
     CDS             6128..6277
                     /locus_tag="ACG8WP_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028212.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG8WP_001916"
     CDS             6352..6945
                     /locus_tag="ACG8WP_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9028213.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG8WP_001917"
     CDS             6958..7857
                     /locus_tag="ACG8WP_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9028214.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8WP_001918"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8WP_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9028215.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG8WP_001919"
     CDS             8723..8983
                     /locus_tag="ACG8WP_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028216.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG8WP_001920"
     CDS             9428..9634
                     /locus_tag="ACG8WP_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9028217.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG8WP_001921"
     CDS             9655..9885
                     /locus_tag="ACG8WP_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028218.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG8WP_001922"
     CDS             9901..10338
                     /locus_tag="ACG8WP_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028219.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG8WP_001923"
     CDS             10331..11038
                     /locus_tag="ACG8WP_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028220.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG8WP_001924"
     CDS             11202..11402
                     /locus_tag="ACG8WP_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028221.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG8WP_001925"
     CDS             11418..11597
                     /locus_tag="ACG8WP_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028222.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG8WP_001926"
     CDS             11922..12185
                     /locus_tag="ACG8WP_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFP9028223.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG8WP_001927"
     CDS             12179..12529
                     /locus_tag="ACG8WP_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9028224.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG8WP_001928"
     CDS             complement(12553..13167)
                     /locus_tag="ACG8WP_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028225.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG8WP_001929"
     CDS             13481..13903
                     /locus_tag="ACG8WP_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028226.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG8WP_001930"
     CDS             13913..14116
                     /locus_tag="ACG8WP_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028227.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG8WP_001931"
     CDS             14327..14911
                     /locus_tag="ACG8WP_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028228.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3061 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaag
//
LOCUS       DAZKAY010000065        15046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000065 DAZKAY010000000
VERSION     DAZKAY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.065"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1531)
                     /locus_tag="ACG8WP_001932"
     CDS             complement(167..1531)
                     /locus_tag="ACG8WP_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9028229.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1528..3087)
                     /locus_tag="ACG8WP_001933"
     CDS             complement(1528..3087)
                     /locus_tag="ACG8WP_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028230.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3143..5623)
                     /locus_tag="ACG8WP_001934"
     CDS             complement(3143..5623)
                     /locus_tag="ACG8WP_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028231.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(5607..5942)
                     /locus_tag="ACG8WP_001935"
     CDS             complement(5607..5942)
                     /locus_tag="ACG8WP_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028232.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5956..6990)
                     /locus_tag="ACG8WP_001936"
     CDS             complement(5956..6990)
                     /locus_tag="ACG8WP_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9028233.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7080..7760)
                     /locus_tag="ACG8WP_001937"
     CDS             complement(7080..7760)
                     /locus_tag="ACG8WP_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9028234.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(8044..9147)
                     /locus_tag="ACG8WP_001938"
     CDS             complement(8044..9147)
                     /locus_tag="ACG8WP_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9028235.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9369..9839)
                     /locus_tag="ACG8WP_001939"
     CDS             complement(9369..9839)
                     /locus_tag="ACG8WP_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9028236.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10172..11812
                     /locus_tag="ACG8WP_001940"
     CDS             10172..11812
                     /locus_tag="ACG8WP_001940"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9028237.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11966..13288
                     /locus_tag="ACG8WP_001941"
     CDS             11966..13288
                     /locus_tag="ACG8WP_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9028238.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13350..13781)
                     /locus_tag="ACG8WP_001942"
     CDS             complement(13350..13781)
                     /locus_tag="ACG8WP_001942"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028239.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13845..14681)
                     /locus_tag="ACG8WP_001943"
     CDS             complement(13845..14681)
                     /locus_tag="ACG8WP_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9028240.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4397 a         3048 c         2718 g         4883 t
ORIGIN      
        1 ctcctcctcc tgaaataatc attttataca acaattttat cacttgataa atttttggcg
       61 aatattatgc tcaaaaaatt cttccgctat ttcattatat gaatttttta ttctcgattg
      121 attaatattt attatatccc cacacaataa aagaccgtga tatttctcac agtcttttat
      181 ctactctatt gctatttgct ctcttagcta gtactttaaa agctaactca ttgaggtagt
      241 gaaaacgaga ggtgtattca gacagaaatc caaaatcctc aactgctagc tgtggaatcg
      301 tagttttgtg tttcacgata tccgcaagca ggttcaaatc tgataataag aattcttggc
      361 ttatcagctg tccgccaatt atttctaatg tatccggatg gtagatcagc tttgcattta
      421 ctgttttgtt ttcttcgtcg tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg
      481 tattttgttc caagaatggc gcttcatctt ctgtcagccc tacagttcct aaaaagtagt
      541 ctataatgac tgctgctgtt atcttttgtc tgaggtcgta agcggttggc tgaccactca
      601 aagttagcgc tactgctctt gccatatgta cagctcgagt aactaaaggt aaaaagagat
      661 gccgtttatt tttgtcaaaa ctgactggga cgatatctcc aatcgcatag atgtccggat
      721 cacttgtctg cagatattca ttgactatca cagtctcatc ctcatacaaa gccacttttt
      781 cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa aatgaccata tctgccttta
      841 ttgtttgaga cagaagttgt acggatacta ctttctcttc tgattttttc caatcgatca
      901 agaattcatt tggataaaaa tttatttgct tagtttgcag ctgattttga aaactgttga
      961 tcatatcctt atcgaaatat cttgataaaa cattcggcat ataatcaact agatgaataa
     1021 tgtatccttt atttctcaaa gcatctgcta attccaatcc aatgtaacca gcaccgaaga
     1081 tcactacttc ttttgcttct tttaaagcgg caagtacttg cttagattgc actagatttt
     1141 tataggtgta gacattttca gcttcaattt gtttatacaa ggtgacagga gaggaacctg
     1201 tcgctacgat taatttatca taggttatgg tctcgttttt tccttctttg gatgcgagga
     1261 tgagctgatg tttgtctgga tcaagttcag ttactctcca ttcggtgatc agttttgccc
     1321 cactttcttg aagagtccgc atgttatggg caacttcagc taaactacgt atctttcctt
     1381 ttaaataaag gttgattcca tttgaaacat agcctagtcc atctttatga tcttcatcga
     1441 tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt tagggcagct gtgatgcctg
     1501 catgggaagc accaatgatc acgattttca tgttttattc atccttaaat tcaatttttc
     1561 taaataacat tcttttaaaa attggttcaa ataggggaaa tcataattat attcgttgct
     1621 agtgaataca aaaactttag cggacttctg tgacgggtac tctttcaatt ggctagtcaa
     1681 aatgatatca tactgctttt ctttctcgta ttcgactaaa tcatatttga tcgtattcag
     1741 caattgttcc tggatgattt ctttcacgat atccgccatt agcctttcat aaggaagatc
     1801 ataagcaatt tgcagacgta attctaactg cttgatcagc gtccgtaaaa tagatgcata
     1861 gcgagcaaat aattcaaagc gggcattttc ctcgtctact tcagtaaaat caacaatagc
     1921 aaatgtttgt ctgaccaatc tttcacataa aaccaggaat tgtttatctt ctacttcagt
     1981 taaccgctgt tttaatcctt cttggccaaa aatattcatc catcctctaa aataggcaaa
     2041 atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac
     2101 ataaaaagag aaatattctt ctatcgattg tttaaacgtt cgattcaatt gagctactac
     2161 gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa
     2221 tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt tcattttgat tccaaggaaa
     2281 ggcatattga aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt
     2341 ttgaaacaat ccaacaggaa gattgctata atctggtttg gattttttcg tacagcgcat
     2401 tcgcttcttc ataatggaaa gccatatttc aaatttcaat cgtccttctt ctgtaatagc
     2461 aaaactaaac tctttttgca gcagatctaa aaaagtgttt cctgataaat cactgtattc
     2521 ttttttgttt tcaagatagg gacgtccaaa ccaaaataat tggaaataaa aataacgaat
     2581 ttgactttct ggaccggtca gccgcccgct ttttattttc agatgaaact catttaaaga
     2641 atcatttaat tccttcactc tgcgagacaa agtagccgaa ctgatattaa gttccatgac
     2701 aagtttctgc cgatcaaatg agccattttc caacagataa agaagtatct gatattttgt
     2761 agaatagcgc aagtaacaat gataataaac atcgactgct gctatatttc catgtctgat
     2821 taataaaata ttcccttctt ggataacaat ttccaattgt ttgatatttt ccaagttagc
     2881 ttgtaaataa aacagatctt ctttcaaggt agcccgatca actgataagc gggtaagtac
     2941 gttattaacg gaaacctctc ctttttcctc taataaaagt tggaaaagtt tgatttgccg
     3001 ttgttcattt ttttcaagca aactgaaaag ttccaagtcc actccttcaa gcatttctga
     3061 ttttaacagc agaagtttcc ttctcatcct tgaaaaagag gatctgtgac agttatcaca
     3121 gatccataca aattctaatc acttagctgg ttggcccagc tgtcagtttc catgtgacca
     3181 tgccttgata cgttgcgcca ggttgagcat tcgtactgct atttacctct aagagtaaac
     3241 catcggtatc gggaaagtca acgagaacct ctcctttagc tgttccatca ctctcataaa
     3301 tcaacgtgtt ttgaccttgc tgaatcaatt gctttgatcc agaatgattg aagtataatt
     3361 ttgttccgct taattcttga ccttgatcat tcttaaataa agaagtcaat gtacctgtca
     3421 gttgccaagg agacgtgctt ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt
     3481 ttgattcata ataacttgtc ttcaacggtt tggggagatc gttaccaaat gtaaaatcat
     3541 ctggtacatt aactaatgtt aattcttgtt tggggacaag aattgtttta gcagatgttt
     3601 ggacaactgg gttaggaagc ttagcaccat ctgctaatgt aatattgctg gaaagtacag
     3661 ctgcctttgt cagtatgctg acggattgta aagaagtatc tacagtattt aattgatagt
     3721 tgtaagtaag atgaatggta tctcctattt tgctcaatag atctgctggg acagaaactc
     3781 cttgttgaat gccggtatct gcgatagttg taccatttaa ttttaaggaa ccattgatat
     3841 aagtagtatt ttcaggaagt gagatcgtga tttttttgtc ggttatcgca tccccagatt
     3901 ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca gacaggggat
     3961 ctcctgtctc aggttgtctg ctaaggtttt ttagctgact atctgcactc acagatggaa
     4021 aattagccac tgttatttga ggaagagtgg tgattccagc ttgttcagac tgccatttgt
     4081 acgcaaaaga cggatacatg ttagtaagta catcgtccac caagcgataa tcaaaataat
     4141 attgccctga agccaatgca ggtagtgtag aagtagctgt atttacaccc ccgttatttg
     4201 attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg gttcctttcg
     4261 aataaagcct gtaatggatc gaaccctttt ttagttcagt tgctgaccaa ttggccgtga
     4321 cttggtacga tgcagaagaa aggagctttc cccactgact atcggctgtt aagtttgttg
     4381 gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt taaagcaaca ggagaaagca
     4441 atccgccttg cgcatctttc ccactgtaca cgatattggc tgtctgattc aatccacttg
     4501 tgcttgctgg agtatgagtc agtattgctc cttgtgtaac gttttttcct gtaaaatagt
     4561 tgactacgcg atcaaagcga ctaaatggca actgatcaaa acgtccttca gtcagcccca
     4621 tcgtcaatgt ccgactattg gtagagcctg gaaggttctg gttatttcca tccacgtatt
     4681 ctgtggtatc agcaaatgta ttatattttc ctgttgaatc acttggtgag atgtaactat
     4741 tccacgtatt tatctctgca cttgcagaaa atgcatatgc tcctggattc aagtttccgc
     4801 cagttaattt aggataaaat ctacctgcag ctggagctaa aatcgaattg aacatacgat
     4861 cttggataat attattcgaa tcataaaaat caacagaaaa aacggccttt ttagacatag
     4921 aaggttgtaa tttacccttg gtcaaaattt tgtttggact cgtatcatat ttatctccgt
     4981 tttctgtcat cttcgcgaca ttagctgtaa catcaaattg aagcgggata tcgtccacgt
     5041 tgaattcgcc tcgcgtaata atagcaataa tatctgtcaa tagagtattt gtatcccacg
     5101 accccatccc aaaacgcaag gtatggcttc ctgctgataa taaacttccg ctagtaacct
     5161 tgatgggggc accggcaata tacaagtagg cgttgttcca gtcaattgct tttaatacat
     5221 cactgcttga tacatgttcc ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag
     5281 tgatattcac caaatcaagt acatttgtta gtttcacatg gctttcccaa tgaaaacgga
     5341 ccgtgccacc tttccaagga tcatttgaac catcgttcaa ataagtagca gtcaatttag
     5401 gcaattggtt agccccagtt agttggacaa gcttcccgcc attgactgca ctacgtccac
     5461 catttggagg tacgatattt tcatacgtgt atggatcata agaaatggca ggaggcgttg
     5521 ctccaacagc ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa
     5581 aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc ctccttttcc
     5641 tttcttctcc ataacaacaa ccacgcaaga agccataatt ctataccaat gatcaaataa
     5701 agaaaacgga tcttttgttc attcgtctcc gggaatacgc catgttgatt agattcttta
     5761 taaatcggta gctgtgtctg ctgttttgat gcgccattga ctgaaccact tggatgcgga
     5821 attggactta gatcataaaa tgtaatcgta cctgaacttt tggtatctgc ttggactggc
     5881 tcttgccagc tgaagaacag caaacttgca ataatccctc ccaataagat cgatgttttc
     5941 aacgaaatca cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa
     6001 cagcaaaatc aaccataaca ctagaactgc agcaaccaat cctgcataaa gccatgggaa
     6061 agattgttct tgtactgcaa aaacggaatt ttcatttact ttttgtgctt tttctttttt
     6121 gatcgtaaag ttttgtgtaa atcgccattc attactgtcg cctgattttg ctatgatttc
     6181 tgcgcggtaa tcgccggctt ttgctttatc accagctaaa tcaagattca agtgaaaatt
     6241 cgaatttggt gcaactaaaa atgcattaga cgtattttca atataaagct gattttcata
     6301 aaagatcttg ctcgtaaata tcaaattgct gataatccta ggctgaggat tttggaaacg
     6361 aagctgaaca gttggctttg cgttgacctc gtccaaatct cctgataaaa gtttcagatc
     6421 cggttctatc gtctcttgac tttcttgtaa aacgactgcg agattataag caatacgatt
     6481 tttgatttgt gctccttctg tagcattttc tgtttcagct tcttctttag gtgaaacatg
     6541 aatacccgct aatacacgtc ccttaaagct ttttgctggg attttcactt ctataggtac
     6601 agttgtttct gcatgtgctg atacattgat ttcctttttc gcaatgagca aaacgtcacg
     6661 gacatcaaac ggcaatgtct catctaacgt tgggtttttt tgactataat cgatcgttcc
     6721 accatcactc gttaccgccg gattgacaga gacctcaaat gttcgttctt catctgaaga
     6781 attgctgata attacttcta gtgtctgatt ttgttccggt gccactaata aatcaaaata
     6841 accgattgct ttatctattt ggttctctgg aaataccggt ttgattccaa agtctcctgc
     6901 tgaatccttt gcttggctga ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa
     6961 cgaaacaaac agtcgtttac tttgattcat tgtccaccac atcctttcaa caaaaactgc
     7021 ggtaaaaaat ccttcaccta tagtattttc accgcagttt gttgaattta tttcatactc
     7081 tatgctactg ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct
     7141 gctttcggca ctttcagtgt gatatttgct ggatcataaa cagaaagcca tgttcccaga
     7201 ccttgatctt ttgctgctgt aaatattggt gtcgctgcac ttgctttact caacccagtt
     7261 acagtttgac tagttggtcc agtggattcg ttatctgacg tacttttaac agtggatgtt
     7321 ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa
     7381 actgtcacgt tccacccttg tgcttggcca tctgcaccta cacctcgacg atctgatact
     7441 tggagataag gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt tccgctttca
     7501 atctcgtgtg taccaaatgg caattcggga actacatcca gtgttaaagg acctgtctgg
     7561 ccagttgctg gattttccgg atctgctggc tctactggtt ttgaaggatc agtagggtta
     7621 agaggctcca cggtatttgt tggttcagta aaggtaatat ctccttttga agtcgcttga
     7681 ccctcagcaa aagctggtgt tgccaagccg cctagaagaa gtgttgaaag taataatgtc
     7741 ccaatcatgt tctttttcat aataaatcac tcctttttct tagtgaccta agtatagtag
     7801 agacagtatg caaaaaatcc tcatgaattc atccgcttac ttgaaattat gacccttttt
     7861 gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt ttttattaag
     7921 tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt
     7981 gaactataaa aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttgt
     8041 ttcttaaata aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt
     8101 tccagctaat tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac
     8161 tcctgtactt cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc
     8221 tgcacctgat gcgattgctt tgaaaacatg ctgtccgcgt cgtacaccgc tgtcaaacac
     8281 gattggtaca cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgctgctgg
     8341 accaccatct agttgacggc caccatgatt agatacccag attccgccag caccagattc
     8401 taatgaacga tagacatctt cttctgactg gacacctttc acatagactg gcaaatcaga
     8461 atgagcagca ataaattcta catcttttgg actaagcttc tgtttggaag atttgtacac
     8521 agcatccatc gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagtgg
     8581 gaaggtaaac ccatttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt
     8641 caatacgatt gcttttgctc cattacgctt ggccacttca agaatatcca aattgatccc
     8701 atcatctttg ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct
     8761 tatctcttct aatgtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc
     8821 tacacctttt gcagaagctt tctccgcctt cacatgagct aagccatgag cagcaactgg
     8881 tgccatgatg attggtgcag tcagcgtttc tttgtcaaaa tgtgtagttg tatcgggtaa
     8941 ttctacatct tttaatacat gaggaataat cagctgatga ttgaatgccc gttcattttc
     9001 ttgataagta aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat
     9061 cacttgtgaa gcggctattt ctaaatcttc catattgata aaatccacta ttccttcatt
     9121 tgtacctgct tgatacgttt tttccatttc aatgccttcc ctctataaaa aattgaatga
     9181 ataatatgat agcgtttaca cactttatat tactcttccg tattgataca ggcaaataat
     9241 ctgtttacat ttcagtcatt ccagttgtta ctactctgaa agaaatgttt agaacacaaa
     9301 tagataaaaa acgatgggac tttgacaaaa gtcgtgtcaa agtccctttt acgaaatact
     9361 gtggatgcct attgaaataa gtgcgggttg tttctttgta tttcaatcgt ttcttgccat
     9421 tcttcgttta attttggatt accgttttct tccttgaacg cataaggtcc caatccaaaa
     9481 agctcagtta gtatttgatt cacgattcca tatagctcgg tgtgatcgga cacatgatta
     9541 ttgaagatca tctcattatc accacgtttg aatctcacat tcgggctctc atgatgaacc
     9601 tctatgcagt tttccccttt ttttaactga cggatttctg aaaacatctc ataaatatct
     9661 tctccaataa aaccttcagc aagcgccaga ctgtccacaa gaccgcctac tgtcaactca
     9721 gagattatcg ctttgttggc accaagcttt aatgtatagc ttgttgcttc tactacgccg
     9781 catgcattgc aaccaccttc tgatagttcg ctttttctga tcaattgaac ttctttcatt
     9841 ttctgttcct cctcttagtt ttctacttat gtatttttta tcacctgacg taagtgtatt
     9901 ttatcgtcac gatcacgtga tttattcttc agagattcta tttatctatc taacatatcc
     9961 tttattcttc tacttttctt tctttagcat ataagagata cgcatagtaa tcaagcaagg
    10021 attgttgata taaaaagaac aaataccgct attttgattt cttttgactc tattactcat
    10081 ataaataaaa tttattatac ctgctctttt tgactatatc ctcatctgac tcctatactt
    10141 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggctaaact tattaaataa
    10201 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat acggaatcac
    10261 tggtatgctt ccaagtactg tccaaacatt ggaacaacaa tctgtccaag cttacggtca
    10321 atacttaagc aaacagaccg atttggaaaa acgtatcttc ttaatgaatc tattcaatac
    10381 gaaccggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
    10441 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccaca
    10501 agatgctgca tttctttcaa tcgacgaacc agaaaatatc aaagcaagtt tgaaaaacgc
    10561 agcagatgga cgcgacattc gtttgatcgt tgtgacagat gcagaaggaa tcttaggtat
    10621 gggtgactgg ggtgtgaacg gtgttgacat cgctatcggt aaattgatgg tctacactgc
    10681 tgcggctggt atcaatcctg cacaagtctt gcctgtatca atcgacgctg gtactaataa
    10741 cgaagaatta ttgaataatc cattatactt agggaatcgt catgctcgtg tagaaggcga
    10801 aacttactac gaattcattg atcaattcgt tcaatcagct actgaattat tcccagaatt
    10861 gttattacat tgggaagatt ttggacgagg aaatgctgca acgatcttag aaaaatacga
    10921 agataaaatc actactttca atgatgatat ccaaggcaca ggaatcgttg tactagctgg
    10981 tgtattaggt ggattgaata tatcaggtga gcaattgaaa gatcaaacga tcttgacatt
    11041 tggtgctggt actgctggtg taggtatcgc aaacatcctt ttagatgaaa tgatccgtca
    11101 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac agggtttatt
    11161 gtttgaagat acaaaaggat tgactcctgg tcaaatccca ttcgctcgta aacgctcaga
    11221 attcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcaa tccatccaac
    11281 gatcatgatc ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
    11341 ggcggctcat accccacgtc caatcatcat gcctttgtct aatccaacaa aattggcaga
    11401 agcaaaagca aaagatttga ttgaatggac ggacggaaaa gctttagttg gaacaggtat
    11461 ccctgctgct gatgtggaat ataacggtgt aacttaccaa atcggtcaag caaataatgc
    11521 gttgatgtac ccaggtttag ggcttggttt gattgcttca acagctacac gagtaaatgc
    11581 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
    11641 agcagctatc ttgcctccag tagcaaaaat cactgaattc tctcaaacga ttgctgaaac
    11701 tgtcgcaaaa tcagttgttg cacaaaaatt gaatcgtgaa gaaatcacgg atatcaaaga
    11761 agctgttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagattgtta
    11821 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
    11881 aaacagcagc cagccaacta ctgctctttt gccgcttctc tttggctttt ttcatacgat
    11941 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaaccgcaa ctgcagttga
    12001 ggaaaagaaa aaagaagggt tttggtcaac aaaaattacc ggcgtaagtt tgccccttta
    12061 tttgatcatg gtccttgtcc tgatcattgc aatggcttta ggaaaattac cgacgaatat
    12121 gattggccca atcgctgttt tggtcatttt cggtaattta tttcatttta tcggaacaaa
    12181 aatccctatc gtcaaaagtt atttaggtgg tggctccgtt tttgcgatct ttgcctctgc
    12241 cgcgattgct acatttggga tcttacctga gtatgtagta aaatccacag aaaactttgt
    12301 cagcaatatg ggctttttag acttttatat tgctgccttg attactggta gcatccttgg
    12361 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
    12421 aatggtcgtc tccttttttg ccgtaggact cgtaggtatg cttatcggaa atggttttgc
    12481 aaattccgtt ttatatatct cattgcccgc aatggctggt ggtgttggag ccggagctgt
    12541 acctttatca acgatttatg caaatgtttt aggtacggac gcttcttcta tcatttcccg
    12601 cctgattcct gcgacagcaa tgacaaatgt cctagctatc attggtgcag cgttagttgc
    12661 tcgtgcggga caatcaatgc caaaattcaa cggtaatggt aaattaatgg aaaaaggcga
    12721 ccttggcgat ggaaaaccta gaaatgtaaa accttccatt ccacaattag gtgtcggttt
    12781 gatggtatca ttgactttct tcatcttagg acaaatctgt aactattttg ttcctaaagt
    12841 ccatgcttac gcattcatga tcattatcgt tgttatctgt aaaatcgcaa acctattacc
    12901 agaatattac gaagatgcag ctatcatgtt caacaacttg attgtcaaaa acttgacagc
    12961 agctgttctt gccggtatcg gtattgcgtt attgaatcta aatgtattgg cttctgcttt
    13021 gacatggcaa tttgttgtct tatgtttgac aagtgttatc gcgatttcag tagtttcagg
    13081 atttgtcggt cgtttgtttg gtttatatcc aatcgaatca actattactg ctggcttatg
    13141 caacaactcg atgggtggta caggaaatgt agctgtctta tctgcagcta atcggatgga
    13201 acttattgct tttgctcaaa tgggcaatcg gcttggtggt gccatcgttt tgatcatctc
    13261 aggtttcttg atgcaattac tttcttagtc aaaaaaaata cctgatcatc aagtatcggc
    13321 ttgatgacca ggtatttttt gataaaaatt tacaggattt tcaaaaatct tttttgcatt
    13381 ttatcggaat gataaccgat gcgttcataa aatcggtgtg cttctactct gtattctgca
    13441 gaattaagtc ttattgctga tattcctcgt cttttcgctt cttctacgcc agtcatcaag
    13501 gcctttccga ttcctctttt ttcagcatgt ttagacacag caagtgccat aatattgaac
    13561 atcggatcgc tgaaaatcga ttcataggac tccgcttgta catagccagc tgctttttta
    13621 gaagtaggat cttcatagac caagaaaaaa tttttttctg gctccttcaa cagtttcttc
    13681 atttgtctca ccgtaacggt taatggtaca tcatatccta attgttccgc attgatttct
    13741 cgtagtgctt ctgcatctcc taaacgcatt tcccgcaaca tccaaatcac cagccaattt
    13801 ttattttttt aaatgaattt ttttatcaca gtatactcct tttttcattt agaacaatat
    13861 gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa gatgacaaag
    13921 gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat aaaggtcact
    13981 ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat aacagcagcg
    14041 gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt taaagcaatc
    14101 gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag tgttgcttct
    14161 tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc accaccaata
    14221 cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca agtccatgac
    14281 gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat
    14341 accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa
    14401 acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg
    14461 acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac
    14521 atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata
    14581 agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg
    14641 ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt
    14701 gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg
    14761 aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa agaggttgtg
    14821 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcataattt
    14881 tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    14941 cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    15001 tttgttgaat attgtgactt gtgaccgagg agttggcagg agaaca
//
LOCUS       DAZKAY010000066        14990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000066 DAZKAY010000000
VERSION     DAZKAY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.066"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8WP_001944"
     CDS             <1..607
                     /locus_tag="ACG8WP_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028241.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            627..1655
                     /locus_tag="ACG8WP_001945"
     CDS             627..1655
                     /locus_tag="ACG8WP_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9028242.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1652..2410
                     /locus_tag="ACG8WP_001946"
     CDS             1652..2410
                     /locus_tag="ACG8WP_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9028243.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2496..3236)
                     /locus_tag="ACG8WP_001947"
     CDS             complement(2496..3236)
                     /locus_tag="ACG8WP_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9028244.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3297..4550
                     /locus_tag="ACG8WP_001948"
     CDS             3297..4550
                     /locus_tag="ACG8WP_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9028245.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4845..5099
                     /locus_tag="ACG8WP_001949"
                     /pseudo
     CDS             4845..5099
                     /locus_tag="ACG8WP_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5221..5547
                     /locus_tag="ACG8WP_001950"
     CDS             5221..5547
                     /locus_tag="ACG8WP_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9028246.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5615..6328
                     /locus_tag="ACG8WP_001951"
     CDS             5615..6328
                     /locus_tag="ACG8WP_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9028247.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6638..7490
                     /gene="licT"
                     /locus_tag="ACG8WP_001952"
                     /pseudo
     CDS             6638..7490
                     /gene="licT"
                     /locus_tag="ACG8WP_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7611..9476
                     /locus_tag="ACG8WP_001953"
     CDS             7611..9476
                     /locus_tag="ACG8WP_001953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9028248.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9490..10933
                     /locus_tag="ACG8WP_001954"
                     /pseudo
     CDS             9490..10933
                     /locus_tag="ACG8WP_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11041..11742
                     /locus_tag="ACG8WP_001955"
     CDS             11041..11742
                     /locus_tag="ACG8WP_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028249.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11868..12380
                     /locus_tag="ACG8WP_001956"
                     /pseudo
     CDS             11868..12380
                     /locus_tag="ACG8WP_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12778..12987)
                     /locus_tag="ACG8WP_001957"
     CDS             complement(12778..12987)
                     /locus_tag="ACG8WP_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9028250.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13228..13803
                     /locus_tag="ACG8WP_001958"
     CDS             13228..13803
                     /locus_tag="ACG8WP_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9028251.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13803..14243
                     /locus_tag="ACG8WP_001959"
     CDS             13803..14243
                     /locus_tag="ACG8WP_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9028252.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14259..14960
                     /locus_tag="ACG8WP_001960"
     CDS             14259..14960
                     /locus_tag="ACG8WP_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9028253.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5065 a         2484 c         3161 g         4280 t
ORIGIN      
        1 agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc aagcaggaga
       61 agctgctgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg ggaatcacaa
      121 tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg ctacgaagat
      181 tgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattgtg gtcagatcaa
      241 agcaactggg ttgacgcaag aagcatttgg ccaaatggat atacaggaaa aattatggat
      301 ttatgatcag cgagaaacaa aaagtccatg gatcttaacg ataaaattat ctcagccttt
      361 cacaaccgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag ctgaggagta
      421 tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa cgaatgtgca
      481 aacagattat tcagaatatt taaataatgg tcgggcgttt aaacttgtct taaccaaaga
      541 aaatcagata gctggtaatt actcagcaga gatgacatgg acattagcag atgcgccgac
      601 agaataaaga tcaaggagaa tgattgatga atcgaatatg gaataaaagt catcgaaaca
      661 actgctcttt acttctgatc cttctccttg gattcatcct ttggcagaca cctgtccatg
      721 cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt gataagaaca
      781 aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc tacacggtct
      841 ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct acgacgaatt
      901 caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca ttaagttatg
      961 atatgaaaaa cttagcaaaa acagtgggag aaataactgt ccctcctcaa agtgcggtca
     1021 ctgagacact ttcactgaaa gctccagaaa aagagctggc tggtgtggtc gcaggaggaa
     1081 tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt gtcgtcaatc
     1141 agttttctca agtgaaagca atcttgattc gaaatagcga aaaagagtct tccccaaatt
     1201 tgaaattaaa aaaaacttat gcggatcaaa gcgatggaag aaatgtgatt aaggcgaatc
     1261 ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa atctacaaaa
     1321 aagaaacaaa agagccttat ctttcaataa aaaaagaaaa aatagatgta gcacccaata
     1381 caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga cattatcaag
     1441 cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat tttgaaataa
     1501 ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat cagccagaaa
     1561 catttgactg gcgatggctg atacttgttg gagtcattct tattggctta tttgctggat
     1621 tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat taagtaccgt
     1681 tctagctgga agtctattat tatgtacaca aagtgttttt gctgagaata agagctcagc
     1741 cactacagaa ggaacgattt ctgtaacaaa accagatgcc gcaaatcctg aagacgttgt
     1801 tccagttcct cctgttgatc caaatgatcc cgatacgccg tttacaccta acgaaccaaa
     1861 tcagggaaca tccggattac tgacgattga ccaagctcca gatcttgatt ttggaacaat
     1921 caagttaggt acagcaaaaa cagtatatgc tgcattacaa aacggaacgg acagtacagg
     1981 gacagcaaca caagtacaga atttcgtaca agtaacggat aaaagcggaa tttatgccgg
     2041 ttggaaacta tcagtcaaac gaacagaatt tattggtaca acatctgata cgaataaact
     2101 aactggttca aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa ctaatagcag
     2161 tggaacgcca tctactattt ctggtactgg cgctgaagga attgagatcc cagcagatca
     2221 ggcagtagat ctagtaactg caggagcaaa tgaagggatt ggcacatgga tttattcatt
     2281 aggagagaat gtggaacaag ggagacaatc agtggaatta tttgttcctg tatccaatta
     2341 tgccgtagac aactacgttt caacattcac atggtcgtta acagatgctc ctacagaagg
     2401 gaaagcgtaa aatcaaatca atgacttcag ggagtggagc cgattcaagg ccaactccct
     2461 gaaatctgct atgcttacgt ctcagctctt attcatcaac tcgaagcagt tttttggaag
     2521 tcatggaaac gttcctttgt cttttcaaag atctttcgac caacgagtaa ggagtcacca
     2581 ttgaaaaatt ctaacacata atgagcagaa tcaattttct gcaattgcga taagttgata
     2641 cagtaagatt ttaatagtaa aaagtaagca tacttttcca actctgtctt caattgtttt
     2701 aaagaagtat tcactaattt atcttctgta gttgttttga ccataacttg gtttttcaaa
     2761 cctttgatac tttcaatata taaaatttcc cgaggataca ggacgagact tccacctttt
     2821 tgatcaataa agattttatc gacatttacg atttgctgtt ctttccaatt gatctgttct
     2881 agcacttgac gaatttgttc agataaattc ctgtcttttc tgatatatcc aatcggaagg
     2941 attttccgat tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa aacaatatta
     3001 ctatgtgcat tttttttacg tatttgttcg gcgacatcaa tacctgtgag tgattgcttc
     3061 aagtcgatat cgataaagaa aacatcgtga ttatcaatag tacccaactg gatcaactga
     3121 ttgatttgag agatagaagt gagctgcacg atattctcga tatgatgatt gaccaattct
     3181 ttgatgatcc attcgctgtg aaactggtta tcttctatga taaaatactt tttcatatgc
     3241 aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg aataagatgg
     3301 tcgcaacagt gacactttat ttgaccttta ctttgatttt agatttaggt atctatttat
     3361 tcttttcgaa acaactgcta gtaaaaaaca gctttggttt tatagctttg atggtggtga
     3421 cacttttatt tcctatccct aaagtgagct ttccttacct agttggctta ttattgatgt
     3481 ttctttttta ttttactacg aaaaacctgt ttatcagctg tttgatcagt aatctttttt
     3541 ttgctatcac tggactcagc ttttttctgg tttttgatct gatggattat ttaggaaaga
     3601 gacatcttgg aacagcaagt ttttttgcac tacttatcgc atttattttc tatctattag
     3661 taaaaagtat agacaaacag acgagatttc tcaacttgct ttttacttat ccaactgaac
     3721 actattttgg aggtatttgt attttattaa gctggggcgt gatttacttg gtattgggaa
     3781 gtgttcactt ccgctcatct aattatctgc ttctatctct tttgtcgata gtcatcagtt
     3841 ttttttcttt gtttttttcc attatgttga tcagtttcca agtaaaagaa agacaacatc
     3901 aggaatcgct tcaactgtat caaataaatg aagaatatta ccatcatcta gaggaattca
     3961 aacatgatta taaaagtcta ctgttttcat taaaaattac tcttcaggaa aataaaaaag
     4021 aaacgttgga atttttagaa atcttagaag atcattctgc agctattttt gaaaaaccgc
     4081 aaatacaaca gctaacaaat attgtatccc cagctgtcag gggtgtcttt ttaaaatttc
     4141 tagaaaaagc agaatcggag aaaatcccga tccaagtagt tattcctgtc gaagtgaaaa
     4201 caatcccgat tgatctggtt gtttttattc gctgtttatc tatttttatt tctaacgcat
     4261 tcgatcatcg tgtagccgat cagagtccga ttacgatcaa tctgctggaa gaaaaagagg
     4321 gattacgttt tagtatagcg aacaaggcaa atccgacgaa tttgacgcta tcggagatgg
     4381 tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg tctatacagt gcgaaaaaaa
     4441 tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag aatcactatt
     4501 tttttgtaga aatgtatctg gcatctagaa agagcaaatc agcctactga cttactgtca
     4561 aaggataagc ttgaggaaag tctacacgga gatagagaca acaacacagc atattcaaaa
     4621 gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt agttaaacaa
     4681 agtagttgac acttccgcta cttagtatta gtacgaaagt atacttcatg atactaagta
     4741 gataagaagt gaaaagcgga acccacgatc gtcaaaatgc taactgcttt gcttacacca
     4801 gataaaggga agagcaatat cgcttatttt aggcgtggag aagcaagaaa atccctacga
     4861 tcacacaaaa aatgctgacc cagcagttaa gagagttaga acaagatggc attgtccatc
     4921 gaaaagtcta taaccaagtc ccgcctaaag tagagtactc gctcaccgaa gaaggaaaat
     4981 cattaagaga gattctagta gcgatgtctg tttggggaga agaacgtatc gaaaaacaaa
     5041 aagcctcagg ggaagaagtc acattactgc atacacatgg tggctaccta aattactgat
     5101 ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc atcatctatt
     5161 ttttatgtaa aaaaatggta tgattaatca agtaagaaat gattggaaga aggcaagcaa
     5221 atgaatagca tggaaaaagt taaccagttt agagatgaac gtaattggcg acaatttcat
     5281 aacgaaaaag atttggctat ttctatttct ttggaagcat ctgaattgtt agaacttttt
     5341 caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa agaagaactg
     5401 gcggatgtat tgatctattc atacatgatg gcagacaacc ttcagctgga tctagatgag
     5461 atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa aagcgcaggt
     5521 gtacgaaaaa aatacacaga gttttagaaa tgtgtcattc ggctgttgga aagagcaaaa
     5581 gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa aagcaaaaag
     5641 atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt tgattttcaa
     5701 tgaacagatc aaggattatc ttgtcagaga gacaggagat aaatatgcta tagcgaatgt
     5761 aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact ttgatgcggt
     5821 agaaccaatg acgacagaag gggtcatgag gtcgcagatg agaggaacag atctaccagt
     5881 gattgctagt atcgcagtcc cttcagtttc aatcaattta ccgattttca aaggcttgga
     5941 taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa tgggaaaagg
     6001 aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgttgttta caccgctgga
     6061 aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt atacatacaa
     6121 aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg aagtcgagga
     6181 aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc gactagttgt
     6241 acaaggagaa ctggaaagtg ttacaccgat aaaagatgcg acagatgata tgagatctgc
     6301 tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta cagattaaaa
     6361 acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa gaggaacgca
     6421 aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag tttttttatt
     6481 ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt tgaataagaa
     6541 atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag taggcgaatt
     6601 ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata aaattttgaa
     6661 taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg
     6721 actagctttt caaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat
     6781 ttattttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
     6841 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
     6901 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
     6961 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
     7021 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
     7081 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
     7141 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
     7201 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
     7261 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
     7321 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
     7381 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
     7441 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
     7501 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
     7561 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
     7621 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
     7681 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
     7741 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
     7801 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
     7861 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
     7921 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
     7981 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
     8041 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
     8101 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
     8161 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
     8221 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
     8281 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
     8341 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
     8401 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
     8461 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
     8521 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
     8581 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
     8641 aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca
     8701 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
     8761 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
     8821 gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa
     8881 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
     8941 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
     9001 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
     9061 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
     9121 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
     9181 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata
     9241 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg
     9301 atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
     9361 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
     9421 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
     9481 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
     9541 aaatcaatta gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat
     9601 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
     9661 tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga
     9721 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
     9781 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
     9841 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
     9901 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
     9961 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
    10021 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
    10081 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
    10141 tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
    10201 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
    10261 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
    10321 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
    10381 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
    10441 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
    10501 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
    10561 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
    10621 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
    10681 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
    10741 tgtagatttg atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg
    10801 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
    10861 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
    10921 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
    10981 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
    11041 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
    11101 tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt
    11161 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
    11221 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
    11281 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
    11341 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
    11401 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
    11461 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
    11521 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
    11581 gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa
    11641 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
    11701 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
    11761 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    11821 tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg
    11881 gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt
    11941 tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc
    12001 gtaaaaatat actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga
    12061 tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca
    12121 tagcaacggg agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata
    12181 gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca
    12241 ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac
    12301 cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg
    12361 atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg
    12421 taccttgaga caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt
    12481 ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga
    12541 aggaaaaatg agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag
    12601 gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa
    12661 aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag
    12721 ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca
    12781 agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg
    12841 atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac
    12901 atcatttatc agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac
    12961 aagtatttgt tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct
    13021 tctataagta agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata
    13081 aaatggctga aaaatgattt ttttctaaat actatatata gtggcgtcta tcctatatac
    13141 cactaaaaaa ctacaaaatc tagtctttta ttttaaaaaa acacgtgtag aatgaagata
    13201 gacttttgag ttagggagtg tttttttatg aaacttactg ttttttgcgg ttcccgtttt
    13261 ggtaataaag aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa
    13321 gaaatcgaac tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca
    13381 gtactggaaa ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa
    13441 ttgcctggaa aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta
    13501 ttattatttg aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag
    13561 gaattctcac aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga
    13621 atattgaata ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa
    13681 gaggatttta tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa
    13741 ctggtcaatg aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa
    13801 taatgagtca aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa
    13861 gtcgtatcga aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc
    13921 atcaccaaga aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa
    13981 aagatatgga agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc
    14041 aaacaatcga tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga
    14101 tgatcattgt acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata
    14161 cgtatttaaa atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa
    14221 cttacgtagg cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag
    14281 gaacgattgg tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta
    14341 aaccatttta tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg
    14401 agcagtacgc ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa
    14461 atgctttagt gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt
    14521 tccatctaaa cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct
    14581 tattagatac gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga
    14641 tggtccatat caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg
    14701 actatgaaca gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc
    14761 gctataatca atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata
    14821 aattcgattt tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa
    14881 aattagctga attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa
    14941 aaggtcaaga attaggctaa aaataaaaaa ccaaacgacg ggaaagaaaa
//
LOCUS       DAZKAY010000067        14440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000067 DAZKAY010000000
VERSION     DAZKAY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.068"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1113)
                     /locus_tag="ACG8WP_001961"
     CDS             complement(226..1113)
                     /locus_tag="ACG8WP_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9028254.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="ACG8WP_001962"
     CDS             complement(1110..1979)
                     /locus_tag="ACG8WP_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9028255.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="ACG8WP_001963"
     CDS             complement(2116..3402)
                     /locus_tag="ACG8WP_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9028256.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="ACG8WP_001964"
     CDS             3675..4253
                     /locus_tag="ACG8WP_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9028257.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="ACG8WP_001965"
     CDS             4253..5182
                     /locus_tag="ACG8WP_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028258.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="ACG8WP_001966"
     CDS             5179..6915
                     /locus_tag="ACG8WP_001966"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9028259.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8WP_001967"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8WP_001967"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9028260.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="ACG8WP_001968"
     CDS             complement(7811..8320)
                     /locus_tag="ACG8WP_001968"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9028261.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8WP_001969"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8WP_001969"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9028262.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="ACG8WP_001970"
     CDS             complement(9621..10712)
                     /locus_tag="ACG8WP_001970"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9028263.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8WP_001971"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8WP_001971"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9028264.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8WP_001972"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8WP_001972"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9028265.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8WP_001973"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8WP_001973"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9028266.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4113 a         3144 c         2609 g         4574 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta
//
LOCUS       DAZKAY010000068        14332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000068 DAZKAY010000000
VERSION     DAZKAY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.069"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..597)
                     /locus_tag="ACG8WP_001974"
     CDS             complement(166..597)
                     /locus_tag="ACG8WP_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFP9028267.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(948..1646)
                     /locus_tag="ACG8WP_001975"
                     /pseudo
     CDS             complement(948..1646)
                     /locus_tag="ACG8WP_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1725..1901
                     /locus_tag="ACG8WP_001976"
                     /pseudo
     CDS             1725..1901
                     /locus_tag="ACG8WP_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2077..2646)
                     /locus_tag="ACG8WP_001977"
     CDS             complement(2077..2646)
                     /locus_tag="ACG8WP_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9028268.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2720..3043)
                     /locus_tag="ACG8WP_001978"
     CDS             complement(2720..3043)
                     /locus_tag="ACG8WP_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028269.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3197..4009)
                     /locus_tag="ACG8WP_001979"
     CDS             complement(3197..4009)
                     /locus_tag="ACG8WP_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028270.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4336..5211
                     /locus_tag="ACG8WP_001980"
     CDS             4336..5211
                     /locus_tag="ACG8WP_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028271.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            5225..5548
                     /locus_tag="ACG8WP_001981"
     CDS             5225..5548
                     /locus_tag="ACG8WP_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028272.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            5545..6297
                     /locus_tag="ACG8WP_001982"
     CDS             5545..6297
                     /locus_tag="ACG8WP_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9028273.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6287..8458
                     /locus_tag="ACG8WP_001983"
                     /pseudo
     CDS             6287..8458
                     /locus_tag="ACG8WP_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8570..10741)
                     /gene="copB"
                     /locus_tag="ACG8WP_001984"
     CDS             complement(8570..10741)
                     /gene="copB"
                     /locus_tag="ACG8WP_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9028274.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10761..12947)
                     /locus_tag="ACG8WP_001985"
     CDS             complement(10761..12947)
                     /locus_tag="ACG8WP_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9028275.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12947..13156)
                     /gene="copZ"
                     /locus_tag="ACG8WP_001986"
     CDS             complement(12947..13156)
                     /gene="copZ"
                     /locus_tag="ACG8WP_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9028276.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13169..13609)
                     /locus_tag="ACG8WP_001987"
     CDS             complement(13169..13609)
                     /locus_tag="ACG8WP_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9028277.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13683..14222)
                     /locus_tag="ACG8WP_001988"
     CDS             complement(13683..14222)
                     /locus_tag="ACG8WP_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9028278.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4085 a         2930 c         2498 g         4819 t
ORIGIN      
        1 tacagtctga atagatttta aatttattgt ccataatgtt attatgaatt tatagcatta
       61 agaaaagaga taagacttta cattttttta ggaatgtaaa gtcttatctc ttttcttaat
      121 tgtacaatta gtattatctg cactactttc taattaaata aatttttagt tattaacttg
      181 attaaaatca gagataatat catttaattc agtttgaagg gttgtaacga ttaaatggaa
      241 attttcttca tctgttttat tatatgggga tgttttcgtt atatataaca acttttcttt
      301 ttcaattttc attaataact ctgtcgaagt ctgtacttta tcttgataat taaatgcaga
      361 cataccactt tgtataattg taattattgc tgaagcaatt gaaacaatca atagtaaaga
      421 cctgtgttcg gaagcctgat caattaatat tgggatacta gctgataaga ctatttttat
      481 aatatttccg ataccaatta ctctattata aaatcgaatt tcttttttta atctatcgat
      541 tgtagaatca atagattcga taaacgcttt ttgatccatc attttctctc caatcatttt
      601 attaaaatca ttaacaaaaa tattataata ggatttaagt aagttctaac taatcacata
      661 taaaaataaa aaaacatttg tattttatca aagttttatt atacttgtca atttgtgtac
      721 atcctaaaat ataagctatt tgtttacttc tgtagaatat atactttgtt gttagataag
      781 ttgcacatgt ttatagaatc taaataataa atttttatgt accgcccctc aaagtgaact
      841 gaaccccaaa agttgaacta tttaatggac tgtttccgat attctactgg agatagtcca
      901 ttaaatagtt caacttttgg ggttcagttc aaagaggggg ggatttttta tcgttgtgga
      961 atatttaaat accaacgctt gtcatgaaaa tcttgtgctc cacccttagt atttccttct
     1021 ggatcgtttg tcgcacgcat catgacgtat actttcttat tagggaaatc acgcatattg
     1081 aaagatacat gatagccaac atttcctaaa gtattataag cttgatttac gtctggtcta
     1141 taaatcccat cagctcttac tctagctagt tcttttccag tattgtagtc cataatgaaa
     1201 atatactcgt atttataatt agcaatgtgc catccagcta catgcaagtt tgcgttttcg
     1261 atttctccga actgatcaat gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca
     1321 gcacctgctc gagttggatc aattactggt ttatcctccg aagtagttgg attatcatcc
     1381 gtaaaaccat gagccaaatc ataggctagt ttttctttac ttacacccat ttcagaaaga
     1441 taaccgtaag gatctgtatg atcgccccaa atattttgtg ttacccataa atgcgatttg
     1501 attcctggtt ggttataagg agtgtctaat gttaatggaa taccatattt cattgctgaa
     1561 tctcttgcca attcaacgta tactttatag ttcttttcaa aagtttcttt atcatgtgta
     1621 tgttgtaact cgatttgcac aggacttttg gctcacgagg ttgtcctact ctacatcttg
     1681 ctgatagata tgctgaaagg aaaacaccaa cattatgttt tgctttttca gctagaacga
     1741 gacaagcagg aataccttta gaagatatca aaggtttttt aggtcataaa gatgtttcca
     1801 ctacacaagt ctatgcccat atttctcccg aagtgaaaaa aagatcaatg aatcaactag
     1861 aaaactatat agaagagcaa ataaaaaagc actcaaattg agtgcttttt taagctcact
     1921 atcactaaaa ctatcactaa ttttatttat aagcgaacat gtttctatca ctgatttttt
     1981 aaatctagcg aaaaaccctt atcttaaagg cgttcgttca attctttggc aaggtcttca
     2041 aagcctggct tgcctagaag tgcgaacatg gttttcttat agctcatgta gaaaaatttt
     2101 cttgatgatg attttatcca tcaattgaga ttgactgttt tgctctccta tgatttcagc
     2161 tatgattgtc cgattttgcg gttttcttct taactggtag ttgattttcc cagtttccat
     2221 caactcgcta agcatatcca cgactccgcc aaatccggtc tttttcgtaa taaaacctag
     2281 tgctaccatt cgttcatcta gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg
     2341 ctgtaatgtt tgggccagct cttccatccc ttcagcattg gatacgacgg aagctgtttt
     2401 gttaataggt tcttcgttta tttcttttgg ttctttcctc tccactttca ctgtttgacg
     2461 aaggacatct agttcttgca agagcgtttc gtattgttct tgggtcagca tcacgccagc
     2521 tactttttca cgattaaata tatatacgcc agttcccgcg tctcgcgctt gagcaaatac
     2581 atccatcggc gatcgtttca cttctgtgat cgaagatgtg ggaacttcta atttctttaa
     2641 ttccaatgga tttcctcctt tactaatcgc tttttattat agcacagact gacttgtttt
     2701 gatctttttt ccaaaggttt tatcggtttt tgcctaacca gtaattgata tcggtaagaa
     2761 gtctttcgat accaaatgaa ggatggtaca gatacgtttt ttgatcttcg ttatggtcta
     2821 gtggaaaaaa atgatcgatg atcactagat ctgcataccg aggcggctta tttcgagtac
     2881 atacatgtat agagatccgt tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc
     2941 ttcttgcttc ttggtgttca tagcgaaaaa aaaagacgat tttcacttca ggagggtgga
     3001 catctaaata ccctcgttcc tggagtgttt tcagtaaaag catcccccac tctactgcgg
     3061 ggtgtgcaag tgcttgttgg atcgctagcc gaaattgata tagtttatct ttggaattgg
     3121 gaaatttttc taatgtttcc tgataataac gaacttcttc tatcagccgg tcatggatac
     3181 caattacgta acgaagtcat tcttggtaaa gtacttttgt cagctttgat ttgctttcgc
     3241 tctcaaattc gctattcagc caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt
     3301 tccgatagtc ttgtgtctct tctatccgtt tccaccgctt ttcagtaatc tggttacagc
     3361 tcaagcgcaa aattgctaga taattttttt cttttattgg caaaatctgc tcctctctaa
     3421 atagcaattc aggaaacaat tcaaagaata gtgttcgact gtctcctaaa atctcgattt
     3481 ctgccggatc ctttctttgc ttcaataatt ggatgaacat ctcagaaaag gactgctctc
     3541 catcatctgc aaaaaaatgc ggaaaatctt tcaaaaagca aaacagcaga tatcttactt
     3601 ttctttctga accaaagatc tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa
     3661 accactttct caatcgctct tgcgtagaac gatcgtactt ctgctcgtaa ggttcattca
     3721 atagcagatt tttcagtaaa caaaacgggg gtgattggat cagaaaaaca aacaagagat
     3781 aaggttcgct atatccttga tcataaacta actgaacggt tttcttatga agaatcagct
     3841 gaatgggaca gtcgctttcc gccaaacgtt tcagatgccg atccactgtt ttaacatcga
     3901 ttttcagttc ttcagctaaa tcagcttttc gccagatcaa atctttcatt agattgatta
     3961 gtgaaaataa attctgtgca ttttttgata atagtttttg ccatctcatc gatcatttct
     4021 cacctgattt ctaaacatat tgtttgatca aaatgggttc actcctaaaa agataaaaaa
     4081 ctccctttct atttatttaa atacgcaaat cttcctcatt attttttatt ttactcaatt
     4141 taatcgtatt ctcatttcaa aacgaatgta ctttaaaaaa acataataag atcactttgc
     4201 ttgtaggatg tattcccttc cattacaatg aaataaatag cgattttatg agaaggtgat
     4261 gtgaaaatgg aagaaaaaat agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa
     4321 ttagaaaaag cggtcatcgg caaacgttca actatccaat tgatgatcgt ggccctatta
     4381 gcaggtggtc atgtcttatt cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa
     4441 gcactagcaa aagctgtaca gggaaccttc agcagagtcc aatgtactcc tgatctgctg
     4501 cctagtgata ttcttggttt ttccgtatac aattcacaat caaaagagtt tgaatttcgt
     4561 ccgggaccga tctttaccac gatcttgtta gcagatgaga tcaatcgtac gactcctcgt
     4621 actcagtcag cacttttaga agcaatggcc gagaatcatg caacgatcga caacaataca
     4681 taccctctag acaatcattt ttttgtctta gctacgcaaa atccaatcga gtacgaagga
     4741 acatatcctt tgccggaagc acagctcgat cgttttttat tccgactaca aatcggttat
     4801 ccttcttttg atgacgaact tttgctactt ttggataaat tcgaaaaagt gttggataac
     4861 ttaaatgaag tattgaacag ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc
     4921 tttgtcagtc ccgaagtggc atcttatgcc ttgcagttag tgtcagcaac aagaagtcat
     4981 gctgccatcc agcttggcat cagtccacga ggaagccttt cctttattca ggcagcaaaa
     5041 gcttatgctt taatccatgg gagaaattac gtcacgccca aagatttgca ggatttagtt
     5101 ccttacgtgt tttcccatcg cctgattttt tacgatcgtt cattgaagga agacgaaaag
     5161 aaacaaatca ttcatactct tgttgcacaa attcctattc ctgtgaggta gattgaggta
     5221 gacaatggaa aaaatcaaag attatttcat taattttgtg ttatttctgg tatatttcat
     5281 cattgtgctt tttgctcttg tattcaacaa ttctttaggc tggttcttgt tggccttttt
     5341 tactctttat gtatcatgga gcttgatttc attaagcagc tttctaaaga aactttcggt
     5401 tacttgtaca ttgcagccac tcgtttatcg caaaaaaagt atgcaggtta ttttttccat
     5461 agcaaaaaag agaacttttt ctttacctct ccctcgttta caaatcggct tcccagcaat
     5521 ctttgcagaa aaaaacaaga gattttgatt cttacaaata aaccgcaaga agtccaattg
     5581 ctttggcagc caaaagaaag aggatgccat gaaacattaa ctgtcatttt tacagcctat
     5641 gatagtctag gacttttcag aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt
     5701 ctgcctgctt tttgtaagat tcaagcagaa aaattacaga gagttctgga aaaaaaacag
     5761 cagctgaacg cgtaccaaag aggtcttgat tttcgggaac atcgcgctta tcgtccaggt
     5821 gattctttca accgaatcga ttggaaattg tctgctcata ctcaagagtg gttataccgt
     5881 gaatacgatt atcaagaaga agaatgttct cctctttttt gcttttgggg ttcgcccagt
     5941 ccaaaatttg aacctctgct agacttgtat ttttctttat ggcatttgag aaaagaaaag
     6001 cagccggaac ttctgatatt aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc
     6061 tattttgctt ctttggaagc tggcgatgag aaagcttttt tcgcacactt gaaaaaatat
     6121 gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc ctgaagtctc agaagctatt
     6181 gaaacattgt caaaagatcg aacggtttat cttgtttatt tcgtagaaaa acaactcatg
     6241 gtccaagaaa aagacaagat atgttcattg ccaggaggtg aatggattga cgattaaacg
     6301 ggaaaaatgg ctgcgtggcg tttactcctt tgtgatacta agtgtaggtg ctccgatatt
     6361 tgtagtagct aatcaattgc aaaacagcta ccttcttatc ctaactattg gtgtcctatg
     6421 ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat
     6481 gataggttgt ttgtttttat attttccttc gaagacattt tccatattat ggatcttaca
     6541 gtttttttcg tcagtcacag aggaattctt ccatctatcc caaggtaaac tttattattt
     6601 gccagaaaag acagcttttt ttctcataat gttgctgatc tatttattta ttacattgac
     6661 cgtggattac gactattggt acgcaccctt tctttctttg atggcttact tcatgatgct
     6721 taccgttttt cggaaaaaag atttactgta cgaaatgatt gcggtagtaa ctgttttgtt
     6781 cgtgtatctg gcagcaaggc aatttttctc catccggttg ttgtcgggaa acactcgatt
     6841 ccagtcattg actacagttc tgcttatttg tttcttaact tttagcgcta tcttgccttt
     6901 atatcttccg aaagtacacc aatctctcga ggaaacgagt gaaccgatcc gagaatatct
     6961 taatgatgaa gggctttatg acacactcca tgagtatcaa ttaaccggaa gtgcaagaac
     7021 aggcttcagt gagaatgatg aacagcttgg tggaccttta tatgataatg gcgaagttgt
     7081 ttttacagcg aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa atcattacac
     7141 cggttctgga tggatagaag atcaggacgc acccatccaa ccaatccaag aattccctgt
     7201 tgacatcacg agctaccaaa ccgttataga agacaaagaa aacattacat tgaccctaaa
     7261 aaaacaagtt ttcttacctt atccttatgg aaatactacc ttgactgatt tttcattagc
     7321 tagtggacaa acgctttact atttgcctga aagccagcga ttcattcctg tcccgaatga
     7381 atcgacacgt tctgcaactt tacaaacaga aaggttagtt tttacgagag aagaattaaa
     7441 aagcagtcag ttaaaagctg atgatttaga aaatcagcag acaaatcttc aattgccaaa
     7501 aagtttgcct aaacgtattg aggacttggc ggtcgaattg accaaaaatg aagcaacttt
     7561 aattcgtaaa gtcgaagctg tctaacagta tttaaaaaca agcggaaatt tccgctattc
     7621 caaaacggat accgggcata ctccaaaaga acacgattat gtggattatt tcctctttga
     7681 ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc ttcgaacact
     7741 tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag gcacagataa
     7801 tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc cagaggtata
     7861 ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca atccaatggg
     7921 ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac aaccaaatga
     7981 aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc catcagaaga
     8041 aacaacactc acaccagctt ataaaatcgt cttattcagc cttggagtgt gtttatccgt
     8101 cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt atcttatcta
     8161 tcttcatttt tttgcttcta ctaatacgat tgcatatcaa aaacttctta accctttgga
     8221 aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca
     8281 agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag cagaaatata
     8341 tggacgacaa gcagatccgg taaaattcga taaaaaaacc tatttagaaa taacgcatat
     8401 aataagaaaa atggtataca aaaaaattct gacaaaaggg agccgcttca aaaaataaga
     8461 taaaatttct ggagaatcta cttctggaat gccgagaacc agctgattga gtatcgatta
     8521 ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat tatctgattt
     8581 tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag acagcaccaa
     8641 ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc ggaattgcaa
     8701 gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc gtttgcttgg
     8761 ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg acatccgcag
     8821 aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca cgggctaaac
     8881 ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg tcaaggtatt
     8941 tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct tcgatcccta
     9001 aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg acaggagtga
     9061 tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact acgtcaccta
     9121 aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta tttccttgtt
     9181 cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca acttcttttt
     9241 cattaactat tttcacttgt tgttttttga ctgttgcctc tagcccgact cctgaaagat
     9301 tttttagatt ttcagcttga taaggcttga tttcatgttc tttcaaataa ttcataatgc
     9361 caacagccaa tggatgattg gcattcgctt ccaaggctcc tagatattga aggatttcat
     9421 ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt ccttctgtta
     9481 gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc agtgcattgc
     9541 ggtttttcaa aagtaatccg tttttagctg ctattgatgt ggaacgagca acaactaatg
     9601 ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc atacgttcta
     9661 atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata cccacaatta
     9721 atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc ttggattttt
     9781 ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt tcaccagttc
     9841 cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc actttgtcat
     9901 tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca acaattgttt
     9961 cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg atcaaacgat
    10021 caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc gtcaagcgtt
    10081 tcactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca ttcgacacag
    10141 cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc aattcccaga
    10201 agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa tagacactat
    10261 aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga cttttttgtt
    10321 tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat agtatagttg
    10381 ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga aattccattc
    10441 ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc caaaattttt
    10501 gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga tccatgtgca
    10561 tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc atgtgcatgg
    10621 aatgatccat ctgactatgg tcatgtttca tctccatctg tttttcatga gattctgtac
    10681 tttctttgtt tactacttct tttttatgct catgtttttt ctccatatcg ttcccattca
    10741 tttttactcc atcccttctc ttactttttc atgtgtcgat ttaagcacag ggagttcaac
    10801 aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt caaaaagcct
    10861 agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag attttgtttg
    10921 atttttctca atgtcaacct agacaactcg attgtttgtg caatggatgc caaactgcta
    10981 ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg ttccacttcc cattgccata
    11041 ccgacatcag ctaatgctaa ggccggtgca tcattgattc catcgcctgc cataccgact
    11101 tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg aagtacttca
    11161 gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc tgccagtttg
    11221 ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt gattgcttgt
    11281 ttgacttcta gtttgatttg atcagccacg gcaatcaacc cgatcacttg cttttcatca
    11341 gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc ttggtattca
    11401 tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg
    11461 ttaatcgtac cgctgattcc agcacctggg tgggctgtaa aatcaatcat atcgtatgtt
    11521 gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg ttcagaagct
    11581 tgttctaaag tataaaaaat agataggact tctttagaac cgactagatc tgtaactttg
    11641 ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa gtgagcagct
    11701 gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac acccgttcct
    11761 accataatag ctgttggagt cgctaaacct aatgcacatg gacacgcaat gactaataca
    11821 gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt agctatgagc
    11881 gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg atctgcgatt
    11941 ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat ttgcgctaat
    12001 actgtctgct tgccgacttg tgtaacttcc atgtgaagta gaccattcgt attgattgtc
    12061 ccgccaaaga gcgtgtcatc cggattcttt tctacaggaa gattttcacc agtaagcata
    12121 ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat ctgctcacca
    12181 ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt ttcttctttc
    12241 ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt gattgcatta
    12301 cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag cgtgatgatc
    12361 atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg
    12421 ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt tggtgctttc
    12481 gtcttgattg catggtacgc acctttataa aaacgagcac cgacataaaa ttggacagga
    12541 gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt
    12601 cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt actcaatata
    12661 agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg
    12721 gcttcatcgt acaaaattgc accatagccg atgttttcta cgctttggat taatttttca
    12781 gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac tgtggcattg
    12841 accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt tgcacaagtc
    12901 atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa atcacctctg
    12961 ctttataacc caatccattg attgcttgac aaatcttttc ggattgaact tttgtttcat
    13021 caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc acgccgtcaa
    13081 gtgcgcttac tgtttcttct actcttgctg cacaatgatt gcagctcata ccttcgattg
    13141 aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca cattgacctg
    13201 ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt ttcaaaatct
    13261 cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc gtctctccaa
    13321 tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact gtttccatct
    13381 ccgagacagc tggataataa atgaattttt ttccttcttg ttctgtccag atcacttctt
    13441 tttttaccaa acgacccagc aaagttttga tcgttgctgg tttccaatcc atcgaatcag
    13501 ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata acacgcatga
    13561 tctcccactc tgaatcactg atctttactg gcgttttgac tttttccatt tatttcacac
    13621 ctttcgttta caaatgtaat cattatctta tgaatacaga ttacacttgt aaacaaaaac
    13681 tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt ctctcgtctt
    13741 gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg ctgtattttc
    13801 ggttcattgt tttcttcaaa atgccagata aaagataaaa attctaagta ttccctgttt
    13861 agtttctccg aaaatgattc aggcatatct ggccgagatt ttttgtttat ctttcttttt
    13921 agagaacgcc aaactactcg gtagatggcg agataacgcg gcactttaag ccaaatgatc
    13981 ttatctgctt ctttaatcct ttcctctaat gtagaagtat aatttccttc aacgatccat
    14041 gacggattag ataccataaa caattgctgt tcttgcaaaa accacttttt tgcctgaata
    14101 ctgtagtctg ttgtatgcca taaaaggtct agcggcagta gcggaaagtc ggtgatttct
    14161 ttgatcttcc ttgcgagggt cgattttccc gacccacttt gtccaatcac gatgatcttc
    14221 attcttctcc ctcacttctc tttggaattt ggttcgatca taccacttat aacgccgcaa
    14281 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aa
//
LOCUS       DAZKAY010000069        14261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000069 DAZKAY010000000
VERSION     DAZKAY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.070"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..405)
                     /locus_tag="ACG8WP_001989"
     CDS             complement(205..405)
                     /locus_tag="ACG8WP_001989"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028279.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            528..1130
                     /locus_tag="ACG8WP_001990"
     CDS             528..1130
                     /locus_tag="ACG8WP_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028280.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1185..2351
                     /locus_tag="ACG8WP_001991"
     CDS             1185..2351
                     /locus_tag="ACG8WP_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028281.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            2768..5995
                     /gene="fss3"
                     /locus_tag="ACG8WP_001992"
     CDS             2768..5995
                     /gene="fss3"
                     /locus_tag="ACG8WP_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9028282.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6107..6421
                     /locus_tag="ACG8WP_001993"
     CDS             6107..6421
                     /locus_tag="ACG8WP_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9028283.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6434..6808
                     /locus_tag="ACG8WP_001994"
     CDS             6434..6808
                     /locus_tag="ACG8WP_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9028284.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            6818..7162
                     /locus_tag="ACG8WP_001995"
     CDS             6818..7162
                     /locus_tag="ACG8WP_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP9028285.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            7231..8247
                     /locus_tag="ACG8WP_001996"
     CDS             7231..8247
                     /locus_tag="ACG8WP_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9028286.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            8841..10715
                     /gene="ltrA"
                     /locus_tag="ACG8WP_001997"
     CDS             8841..10715
                     /gene="ltrA"
                     /locus_tag="ACG8WP_001997"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9028287.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            10817..11197
                     /locus_tag="ACG8WP_001998"
                     /pseudo
     CDS             10817..11197
                     /locus_tag="ACG8WP_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            11242..12384
                     /gene="dcm"
                     /locus_tag="ACG8WP_001999"
     CDS             11242..12384
                     /gene="dcm"
                     /locus_tag="ACG8WP_001999"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFP9028288.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12374..12663
                     /locus_tag="ACG8WP_002000"
                     /pseudo
     CDS             12374..12663
                     /locus_tag="ACG8WP_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12919..14103
                     /gene="mobT"
                     /locus_tag="ACG8WP_002001"
     CDS             12919..14103
                     /gene="mobT"
                     /locus_tag="ACG8WP_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9028289.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14100..14213
                     /locus_tag="ACG8WP_002002"
     CDS             14100..14213
                     /locus_tag="ACG8WP_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9028290.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4839 a         2426 c         2976 g         4020 t
ORIGIN      
        1 ccatctcttt tccttttaga aatggtcttt ctaatccggt atcaattctt gcttgatcaa
       61 tagcctcttt tgtcatttca aaaacataat ttttcaattg attcgtttgt tgtttatcta
      121 acattactgt aattcctgca tccattattt ttctcctctc attttctatt tcttcaatca
      181 gccaactcgc caagttgtat ttatttagat tttcaaatca ttaagtaacc aatttaccaa
      241 cttttgataa gtagatgctc taatactttc tttgctccca ttttcgattt tgacatatgt
      301 tttccgattt atacctatca attttgctgc atcgttttta gtaagtataa gatcggctct
      361 atgtcttctt aagcttctaa tgaattgatc attaagagtt atcattcagc gcctcctttc
      421 aaattattca ctttgtggtt tttatcttaa taagcacatt ttaaaccaca aaacgagtaa
      481 tatcaattaa aatattactc aatttgtggt tttcatgcta taatagtttg gaatttacta
      541 tagggggcgt tactttgaat agaataaaat tttatattga ttccagtaaa aagaccatta
      601 aacaaattag tacagaaact ggtattaact atacaactct tacgaactat taccgtggtg
      661 agcgaaatcc tagaaatact gaaacatgga atatccttgc tgattatttc catgttcctg
      721 taagttattt gatgggatta gacgaaacaa attccataga acaacctaat cttgatagaa
      781 taaaaactgc tcgtttagaa aaaaaggaca aaatagtcaa gaatattgcc agtcttgact
      841 cagattattt tactgaatta tctaatctat acttttccat gacttcttta cttgaagtca
      901 aacttcaaga agatgactcc gactttcttt ttttaaaaag tattctagag gactcctttg
      961 aatacacgat tctatttgcc aaacaaaaga agtttattaa taaagcttta aacttaaata
     1021 tcgatgaaag aaaagagtta gaatctgagt tatttggcaa cttaacccaa gtacaggtta
     1081 acaatctaaa aagagagata acagctcgat taatatcaaa acttcaataa aactaattat
     1141 tcaatcagcc aactcgccaa gttatcagat tggagagatt agttatgtca atacaaaagt
     1201 acacaacaaa aagcggtgca cgatatagtg tccgcttgta tgttcgtaaa gatgaaaatg
     1261 gaaagcaaga atactatgta aaaaaagggt ttaaaactga aaaagaagcc aagttacacg
     1321 aagcaagaaa aaaggtcgag attgaagata ccggctatca taagccttct aatgacttgt
     1381 ttataacagt gtatgaagaa tggttgcctt cttataggaa tcgtgtacaa gaaacgacat
     1441 atcaacgtac aaaagacctt ttccgcttgc atatactacc aaaattcggg aataagtcca
     1501 tttctaagat tactcctgtt cactgtcaga atgcagtcaa tgaatgggct ttaaaattca
     1561 agaatatcaa acagcttaag tcttacagct ctcaaatatt tgagtatgcc atttttgcag
     1621 agctattaca ccgaaatcct atggcaaata caaatctacc aaagcgggaa aagaaagaaa
     1681 atgaaaatta cttttcatta gaagaactaa aagctttttg ggatattctc aataaagaag
     1741 agcctttaaa acatatttta atcttccagc ttttgattac tactggtata cgtaaaggag
     1801 agctagctgc tcttcattgg tctgacattt attttgaaga acagctaatg tatgttagaa
     1861 agagctatgc aactattcga aatgaagaga agaatgcaag aagaaaaact gtcaggattc
     1921 aaaaaaatac aaaaaatata tcttccgaac gaatattacc aattgatgca caaactattg
     1981 aaatgttaaa aatctggaaa aaagaacaag ctcgagagct tttgcagttt ggcataaata
     2041 caaatacaaa aaatcaactt atctttacct atgtaaatgc agacggtgac attaatcaac
     2101 ctttgcatgc ggactattca aataatatat tgaaacgttt agagaagaaa tataaattta
     2161 agcacgttac tattcatgga ttccggcaca ctcacgctac tcttctacta gaaggtggag
     2221 caagtatcaa agaaacacaa gatcgattgg gtcataaaaa tgctgaaact acccttaaca
     2281 cttattctca tgttactgaa aaagctcaac gaaatgcgtt agaaaaattc attgcttaca
     2341 ctggtttata atattgtcat tacctttctc attaccagaa cgaaaaaaag acaccttctc
     2401 gcaatgagaa agtgtctgaa atcgttggta taacaacgat taacgttttg aaaactgtga
     2461 agctttacgt gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa
     2521 gccttgattt ataaaggttt ttccgtaaat atattttgtt atatttgatt aaatttgaaa
     2581 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttatattaaa
     2641 tttttaggct cattcattct caaatccatt ctatttagtc aataaatatt ttaaaaagac
     2701 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcctaatt tgaaaggaga
     2761 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
     2821 tgttcaatca attgtaggag caattgctcc tacgattgct tttgctgatg aaattaccca
     2881 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
     2941 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggcgt
     3001 taaacaagat attttctgtg ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
     3061 atatgagaag aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
     3121 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
     3181 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
     3241 agccattgag gcaaaaatca atcaagccat tgccgattat cagaagaaac caagcttcca
     3301 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
     3361 gaatttgtca gagttcgatg aggttgtgga aaataccgca aacattgatt atcgtgtgaa
     3421 tggcaatcag ttgataatca gcccaaatgc caattcaaaa gaaagtggtg tgttgacgct
     3481 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
     3541 aatggccggg gcgatcgata agccaaacac ttatacggtc aaaattgatg tggaaatgga
     3601 gggttctttg aagattaaaa aagttgataa agaatcgggt gatattatac caggaacggt
     3661 tttccattta gactttggaa aaactttacc cgcaaaagac gtgaccaccg ataaagaagg
     3721 cattgcgacc ttagatgaga ttcctcatgg tacaaaggta accattactg aaaaatcggt
     3781 gccggcgcct tatacgattg ataccacacc catgactgcc acaattaaag caggtgaaac
     3841 catttcagtg acttcaaaaa atacgcgaga aaaaggtcaa attattctgg ataaaaccgg
     3901 aattgaaaca gggagtgatc tttggaacga taattactct ttagcgggca atacgtttgc
     3961 cattcgcaag gatagcccaa ctggtgaaat cgtccaagaa atgaccacgg atgaaaaagg
     4021 tcatgcagaa acaccaaaag agattgccaa tgcgttggaa ttgggaacct actatgtgac
     4081 tgaaaccaag gctagtcatg gctttgtgaa tactttcaaa ccagtaaaag tcgaattgaa
     4141 ttatgtcaat caaacagtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat
     4201 tactggtgaa accactttga caaaagaaga caaagatacc ggtcatgaaa ctcaagggaa
     4261 agctgagttt aaaagagctg aatacacctt gtttactgtt aaggatggaa aagcggtcaa
     4321 atggaccgag aactttaaac cagaattgtt gaagggaacg aaagcttctg atgaaaccgt
     4381 aactttgact ttagatgaaa agaatcaagt tgccgttaaa cacttagcga ttaatgagta
     4441 ttactggcaa gaaaccaaag cgcctgaagg atataccttg gatgaaacaa agtatcctgt
     4501 atccatcaaa aaggtggatg atagcgaaaa aaatgctgtg attactcgag atgttgcggc
     4561 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat caactactgg
     4621 cactgccgaa actggcttca atgatctaac ctttaaagtg tcgccattgg aaggaaccaa
     4681 tgaaatcacc ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
     4741 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
     4801 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aagaaaataa
     4861 agaggattat accaagagtg attatatctt tactattacc gaaaaagggc aaaagcaacc
     4921 gattaaaacg gtaacggtcc cttatgggaa tctaaccaat gagaatttct cagtcagttt
     4981 gaaccgtttg atgctttatg atttgcctga ggaagaagat agcttaactt ctcttgcgac
     5041 ttggaaagaa ggagataaag aattgactac tcttgattct accgagctag tcgataaatt
     5101 gagctataat ctacatgaaa tcaaagaaga ctggtatatc gtagctcaag ccatcgatgt
     5161 agatgctaca aaagctgccc aagaaaaaga taaaaaagcc aaaccggtgg cgattgcgga
     5221 aacaaccgca actttagcga ataaagagaa aacaggaact tgggagattc tgcataaatt
     5281 aactgtcgaa caagttttag acaagaccat tgtcttattc aactatgtgt atgaaagcaa
     5341 agaagcctat gaagctggtg atgagccggt agcgaaggat gctagcttga acaatcaagc
     5401 gcaaactgtt aattgtatgg tggaacgtca tgtttccatc caaaccaaag cgcacttaga
     5461 agaaggttct cagaccttta ctcatggtga cgtggtagat atgtttgatg atgtatccat
     5521 tacccatgat gtactagatg gctcaaaaga agcttttgaa acaattctgt acgctttatt
     5581 tccagatggt acaagtaaag aaatttggaa atctggcaag attgattacg aagtgaatga
     5641 caaagaattt accaaaaccg tacttgcgga aaaagtggat acaagtaaat atccagaagg
     5701 tacaagtttt accttcaaag aaattaacta tgacaaagat gggaatatca atggaaaaca
     5761 caatgaagat ttgaaagaaa aatctcaaac tttaacgccc aaaaaagttc cgacaacacc
     5821 aagtacacca gaacaatctg aaatgccaac tgttccaagt ggctctcaag aatctagtcc
     5881 tacagtgaag gcattccctc aaaccgggga gaaaacttcc aatgtgctac tgttcattgg
     5941 ctttagcttg atctttgcga cggctggcta ttatttttgg aatcgtcgga actaaggtga
     6001 taagatgaaa catgtaaata gtagaaaaca atacgcgcga ccgcctccta aaaaagtggt
     6061 cgcgctattt ttgtattcaa aactaacaat gaaaagagga aaaaatatgg aattgaaatt
     6121 tgttgtacca gatatggtcg agacgttcgg aaaactaaca tacggtggtg aaggtgaagt
     6181 tctatcggaa gggtatggtc gaaatactac aattgttggt cgaagttacc atctgttttc
     6241 aaataaacaa cgggccgatg atattgaagt ggtggtagct gcggaagccg gcgaaaagga
     6301 ttttgaccaa gatcaaccac taaaagctgt aaatccccac ttaattgcca aaggctatga
     6361 aattgagaat cgtgggttta ccgattacgt gttgtatgtt gatgatttag tgaaagcata
     6421 ggaggaaaag aagatgagat taacagaagg tattgtcgta gattcaggat taacctttgg
     6481 caagttgcgt ttttccgcat tacgtcgaga agtacgcaag caaaatgagg acggaacggt
     6541 tagtaacgag gtaaaagaac ggacctataa tttgaaatcc tccgcgcaag gtcgaatgat
     6601 ccaagtcagt attccagcaa atgtaccctt acgcgagttt gcttatgatg cagaggtaga
     6661 gctggtcaat ccaatcgtgg atacggtcgc caattatgtt tttcgggaag gaaccaccgt
     6721 taattggttt atcaaggcag atgatttagt cttgaaacga caactgaatc aaggaaaacc
     6781 tacgaaccaa aatgaaggaa agaaataggt gaagaatatg gaaattaatg ttgaacatat
     6841 tttagattgt ctcgatcaat ttggcaaagg tgagctaacg gaggaacaac tcactaacgc
     6901 attaacctat gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg aagtaggtaa
     6961 cgatgccaaa gaagactttg atgtactgga ttggttaggg gaagaagagt ctttctttat
     7021 ggttgtcgag gtaaatgaaa gtttatgtcg gcaggcagaa tctgttttgg aagaaatcga
     7081 tgtggagatg ccggatgcca tcgaaagatt tttaaaacaa ttagtggaaa cgaaacaact
     7141 tcccgtagta gtaaatgact agcataactt tttgaaattg cccaatgaaa tcctattggg
     7201 ctttttcgac tggaggtgga aaccatcaaa atgtacaaag gacatcgcat acgcgcagga
     7261 gatcaacact tgatttatca ctttgttctt ggctggctaa tctttttgtt cattggttgg
     7321 atgagcgtct tttatttaca agaacttaga caatttgata tttctaaggt atcattttcc
     7381 actagtgaaa ttggctggtc tatgaaggat gtaatctgct tgctaggaag tctcgctctt
     7441 tcaggaagca tgatgttact ttatatacac ttttttcagg atcattggcg aagtctttgg
     7501 catcgacaaa agttagctcg gatgattcta gaaaaccatt ggtatgaagt aaagcagact
     7561 caaggtgaag gttttttcaa agatttgaat agtagttgga ccaaagagag tatcagttac
     7621 ttccccaaaa tttattatcg aatgaaagat ggcttacttt ctattcgcgt tcaaatttca
     7681 ctgggaaaat atcaggatca gctattgaag ttggaaaata agttagaaag cggactgtat
     7741 tgtgagttag tagaaaaaga actcaaagac tcctatgtgg aatacacctt gttatacgat
     7801 atgattgcca atcgaattgg gatagaagaa atagtggcag aaaatggggc gttgcgattg
     7861 atgaaaaatc aagtatgggc gtatgattcc ttgccgcata tgttaattgc tggcggcaca
     7921 ggtggtggga agacctattt cttactcacc attattcaag cactattgaa gtcagatgca
     7981 gaattgttta tcctcgatcc caaaaatgcg gacctcgccg atttaggtac gataatgcct
     8041 cacgtatact cccaaaaaga agaaatttct gaatgtgtgg aagattttta cgaacgcatg
     8101 atagctcgta gtaggtcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     8161 tatctcggac ttccgcccaa ctttttaatc tttgatgaat acgtggctta tatgggtgcg
     8221 aatcgttttc ctacaagcat cgaatagatg tgaaatggaa gcctattgag attatcaata
     8281 gtgacaacta atgttttgag agatggaatg aaagccttca agttgagctg aaacatcccc
     8341 gctaatactc ctacatgctc taatcaatga agtagattgg ttttggtatg aagctaggtg
     8401 aagtcgtctg aaatctgcct aagtgcttga gcataaggct gactataggg cgggcggctg
     8461 aaaaagataa ctatggtaag aatgtctgaa aatgactgac gaatctgcga atgtacgggt
     8521 ctaaaaggct gatcttattg aaagatagga acatacggac gtatggtgag ataccgacaa
     8581 gtaaggttcc atgatatgaa aatcgggata gctaacgaag aagctataat ttcacaggct
     8641 catagtagat acctaaggtt atacatgata gctaaaaaca ttggaacgat gtaagcagaa
     8701 aactatcaat taaatgacta cttctagata ggtgcatata agtaataacg aaattgcttt
     8761 gtttctgtga aagtaagggc acagtaccga tgaagcgtct aacaaatgtg gagggatagc
     8821 cctaagtcta tttaataaca atgaatgttg aaacagatga agttcaagga tctggtaaga
     8881 acgtgggaaa atccgttatg gaggtgccac gcttgaacac aaaaatacgt tactgggaat
     8941 attatgatct acaagaaaca ttcgatcagc tatttactca aagtagaaac gggaaaaagt
     9001 tttatcaatt atatgagtta atcatttctg aaaataatat tcttttagca taccgtacta
     9061 tcaaggcaaa caaggggtct tcgacaccag gaacggattc cttcacaatt gataactata
     9121 aggaaatgaa ccaagcagag tttatccatc ttattctcag tcatttagaa aattacaaac
     9181 caaaatccat aaagcgagta atgataccta aacccaatgg tgagaaacgc ccattaggaa
     9241 ttccatgcat gattgatcga atgatccaac agatgttcaa gcagatactg gagccaatat
     9301 gtgaagctaa gttttatgaa catagctacg gtttcagacc attgagaagt gcaaagcatg
     9361 ctctaggtcg cattatgtat ctcataaaca tcagtaaaat gcactatgca gtagacattg
     9421 atatcaaagg tttctttgat aatgtaaacc accgtttact gataaaacaa ttatggaata
     9481 taggaatttg tgacaaacaa gtcttagcta ttctttcaaa atctctgaaa tcacccattc
     9541 aaggagaagg tatcccgagc aaaggaacga tccaaggagg tatcatttct ccactattat
     9601 ctaatgttgt tctaaacgac ttagaccatt gggtctcaaa gcagtggcat acctttgaaa
     9661 caaagtatcc ctatactaaa ggttacaata aatttcgtgc tctaagagat acaaatctaa
     9721 agcaaggcta tattgtcagg tacgcagatg actttaaaat catgactaat gattatccga
     9781 cagcattaaa atggttccac gctgtcaagc tttacctaaa agacagactt aaattagata
     9841 tttccaatga gaagtctaaa atcgtcaatt tgcgtaaatg taagtcggaa tttttaggat
     9901 tcgctatttg cgtgaaacaa aaagggaaaa aatgggtttg taattcacgt atttctaaca
     9961 aaaaaaagga tcagattaaa gaagagatta aacaaagaat caaagatatt caaaagagtc
    10021 ctacagcaca aaatgctctc ctgtttaatg ctctcgtgct cggtgttcat aattatttca
    10081 ggtatgccac tcatattaat ttagatttga ataggatgaa ctacgttctt tcattcacta
    10141 cttacaaccg attatcaaat tgtagtacaa gatgttaccc caacaaagct tccccaactt
    10201 acaaaaagtt ttactcaaca acgatgaaaa cgtataaagt agctggagtc tatttgtacc
    10261 ctctttgcga cgtatctaca aaaacaattt acggatttaa tacggaggat acaccgttta
    10321 ctccgtttgg tcgtcaaaga ttggagcaca aaagcctaca gtctcttgtg ttccaagaat
    10381 taagaaaatt gatggaatcg aaaataccga atagaacggt tgagtattta gatagtagaa
    10441 tatctcgtta ctcaatgaaa tcaggcaagt gtgagattac aaaacaattt ttgccagcaa
    10501 aagccgttca ctgtcatcac tatttgccaa aaagtcttgg tggagacgac aaattcgata
    10561 acttgagaat tattcataag gatatccatc ttctgatcca tacaacaaat aaaatgataa
    10621 tcgatcacta cgtgcatgaa ctgaaactgg tgccagagca aatagcaaaa atcaaccttt
    10681 atcgaaagat gtgtaatctg caaaacattc aatagaaaat aaatagatgg aacgccgtat
    10741 gaggtgaaag tctcacgtac ggtgtggagc gggggaaaag atggagatta tatcaaattc
    10801 ttacctatcg ctatagatgt taacgacaaa agaaagtgcg gtgattttga ataagcttaa
    10861 acaaatcgtg atgttaggcc gtcaatctgg tttctttctg attctagctt gtcaaaggcc
    10921 ggatgcgaaa tatctaggag atgggattcg cgatcagttt aacttccgtg tggctttggg
    10981 tcgtatgagt gagcttgggt attccatgat gtttggagaa gtcgataaaa atttttttat
    11041 gaaacgcatc aaaggacgag gttatgtaga tacagggggc agtgtgatta gtgagtttta
    11101 tacgccactt gtaccaaaag ggtatgattt cttaagggaa attagcaaca ttgttggatt
    11161 aaccgttcat acggaaagag aaaataatag tgtgtagcct aggaatatca ataatcctgg
    11221 gcttttttat tgaggtgatt tatgacattt ttagatttat tcgccgggat tggtggcttt
    11281 cgcttaggta tggaacaagc cggtcaccag tgtatcggtt tttgtgaaat tgatgaattt
    11341 gctcggagga gttataaagc aatccatgat acaaggaagg aggtggaaat gcatgacatc
    11401 acaagtgtat cagacgaatt tgttcgatcc ctcggaccag tggacatcct ttgcggcggc
    11461 tttccgtgcc aagctttttc aattgcggga aagcggcaag gattttccga tactcgaggt
    11521 actttattct ttgaaatcgc tcggttcgcc actcttctcc aacctaagtt tctattcctt
    11581 gagaacgtcc ggggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga
    11641 acgttggatg gattggggta tgatgtggaa tggcaagtgc ttaactcaaa ggcctacgtt
    11701 ccccaaagcc gtgagcggat cttccttatc ggacattctc gagatgcatg taccgaacaa
    11761 gtatttccta tcattggatc gtcttcaaca tctgatcaaa acatcagaaa cttggtaaat
    11821 atcaatcctt ccaatcgagg aatgggtggc caagtctatg gttcagatag cgtagcacca
    11881 accttgaccg gtgatgaagg aattaaaatt gccttaccgg taaaggatgg gatttctgta
    11941 gcgggtatgt tgtcgggaaa ttttgaacaa ggcaatagga tttatgaggt aaccggaaca
    12001 gcccccactt tgtcaacgaa acaaggggga acgaagatta tgattcggaa aaatgaaaca
    12061 gaatatcaag aggtgaagcc aggggacagt gtgaacctgg cttttcctaa ttccacaagt
    12121 cgccgaggtc gtctgggaaa acaaagtgtg cataccttac taacaggaga ccaacaagcg
    12181 gtagtcacgg atcagtacca gattcgaaag ttaacaccta gggagtgctg gcggcttcag
    12241 ggctttccag attgggcttt tgatcgagct tctcaagtca attcagatag tcagttgtat
    12301 aaacaggccg gaaactcagt aacagttccg gtcatttttg atattgctag aagattaaag
    12361 ggggtaaaag catatgattt atgacgagtt gattggagaa atctactgga tgattggcaa
    12421 gattcaggca gatccgaaat tggaagaaga actgtaccgg ctcaattttg atattcgcaa
    12481 aaacggggtg aaagtccagg tgattcgtat gtagtggatg aggaaacgga tgctcgaatg
    12541 gagctcaatc aagtcatcgg tgaattggaa cgaattgcgg accttgccaa agaacctgat
    12601 atccgccaat acttgtttga gataaaagca gagttggaaa ttgagggtat caccgcggag
    12661 taatagggaa ataacccact gataggcact gaccgcgggg gatttgcgaa gcagaaatcc
    12721 tccgtggtgc tgtgcctgtt catggacggc ggcagccggc catgatgtct caccccccgt
    12781 atctaacagg ggggtacaaa aacacaaaaa acaagcaagt aaccataaaa aatgcttgtt
    12841 gtatcaagga tattaagtaa tttgtatagg tgttaactca tttggaaaag ttaacacctt
    12901 gaatttggag gtgaaaactt ggcgcaacga aatttagatt atcggttatt aaaagaacga
    12961 cgcaacgaat atggcgtttc acaaaataaa ttagctactg cttgcggact tagccgccca
    13021 tacctaaatc agatagaaaa cggtggcgta atcgcctcta caaagaccat gagaaaaatt
    13081 tttgatcaac tggaaagttt caatcccgat ttacctttaa cgttactatt tgactatgta
    13141 aggattcgct ttcccacaac ggatgtgcgg aaaattattc aagaaattct ccatttgaaa
    13201 ttcgagtata tgcttcatga agattacgcc ttttactcct atcaagaaca atatgtcatg
    13261 ggcgatattg tagtgatgtt atctcatgaa gaagataaag gtgtcctttt ggaattaaaa
    13321 ggtcgtggtt gccggcagtt tgaaactttt ttactcgccc aaaagcgtag ctggtacgac
    13381 ttttttgaag attgtctaaa agccggtggc gtgatgaaac gcttggattt agcaatcaat
    13441 gatcgagtgg ggctactgga tattcctgat ttaacgaaaa aatgtcagaa ggaagaatgt
    13501 atctccttgt ttcgtacctt caaaagttac cgttccgggg aactgttgaa agctgatgaa
    13561 aaggatggta tgggcaatac cttatatatt ggcagcctca aaagcgaggt ttatttttgt
    13621 ttgtacgaaa aagattatga acaatatatc aagttaggga ttccattgga ccagaccgaa
    13681 acgaaaaatc gttttgagat tcggttgaaa aatgaccgtg cttattatgc gattcaagat
    13741 ttgttaaaag gtcgtagcat tgaaagtacc acgttttcca ttatcaatcg gtatttgcga
    13801 tttgcggata aagtagatgg aaaacggcga accaattggc ccttgaatga acaatggggt
    13861 cgttttattg gacggaatcg gaaagagatt caattaactt ctgaaccgaa accgtatacc
    13921 attgaacgaa cattaaattg gctaggtaga caagtcgccc ctacgtggaa aatggctaag
    13981 gagttggatc gattgaatca aacaacgtat atccaggata tggtacagaa tgcgcgactc
    14041 tcggaccggc ataaaaagat tttggagcaa caaagtatgg caatcgaaaa cttgattgta
    14101 tgaaaagagg aattttgatg gaaattgtaa aagatatgtt attggtactt ggtggcagta
    14161 tgcttggtgt taccgtgatg tgcttgatga atgcaagtgc agcagcggat tgagcaatgg
    14221 aaaagaaagg aaagtgacaa aatgaatttc ggacaaaatt t
//
LOCUS       DAZKAY010000070        13998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000070 DAZKAY010000000
VERSION     DAZKAY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.071"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG8WP_002003"
     CDS             279..1127
                     /locus_tag="ACG8WP_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9028291.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG8WP_002004"
     CDS             complement(1298..1639)
                     /locus_tag="ACG8WP_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9028292.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG8WP_002005"
     CDS             1725..2432
                     /locus_tag="ACG8WP_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9028293.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG8WP_002006"
     CDS             2419..2742
                     /locus_tag="ACG8WP_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9028294.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG8WP_002007"
     CDS             complement(2831..3868)
                     /locus_tag="ACG8WP_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9028295.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG8WP_002008"
     CDS             complement(4315..5298)
                     /locus_tag="ACG8WP_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9028296.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG8WP_002009"
     CDS             complement(5714..6484)
                     /locus_tag="ACG8WP_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028297.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG8WP_002010"
     CDS             complement(6477..7412)
                     /locus_tag="ACG8WP_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028298.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG8WP_002011"
     CDS             complement(8006..9655)
                     /locus_tag="ACG8WP_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9028299.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG8WP_002012"
     CDS             complement(9850..10746)
                     /locus_tag="ACG8WP_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9028300.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG8WP_002013"
     CDS             complement(10739..11419)
                     /locus_tag="ACG8WP_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9028301.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG8WP_002014"
     CDS             complement(11420..12202)
                     /locus_tag="ACG8WP_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9028302.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG8WP_002015"
     CDS             complement(12195..13112)
                     /locus_tag="ACG8WP_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9028303.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG8WP_002016"
     CDS             complement(13115..13399)
                     /locus_tag="ACG8WP_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028304.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4146 a         3002 c         2445 g         4405 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggttaa acaaaactct
    13921 attttattgc attttacagg aatgtatttc atgcgaaatt aataagtctt tgtttataga
    13981 gttttactag ggtataaa
//
LOCUS       DAZKAY010000071        13897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000071 DAZKAY010000000
VERSION     DAZKAY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.072"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8WP_002017"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8WP_002017"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9028305.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8WP_002018"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8WP_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9028306.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8WP_002019"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8WP_002019"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9028307.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8WP_002020"
     CDS             3461..3679
                     /locus_tag="ACG8WP_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028308.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG8WP_002021"
     CDS             complement(3968..4480)
                     /locus_tag="ACG8WP_002021"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9028309.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8WP_002022"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8WP_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9028310.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG8WP_002023"
     CDS             complement(6933..7376)
                     /locus_tag="ACG8WP_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9028311.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG8WP_002024"
     CDS             complement(7377..8165)
                     /locus_tag="ACG8WP_002024"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9028312.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8WP_002025"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8WP_002025"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9028313.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG8WP_002026"
     CDS             complement(9280..9867)
                     /locus_tag="ACG8WP_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9028314.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8WP_002027"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8WP_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9028315.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG8WP_002028"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG8WP_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9028316.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG8WP_002029"
     CDS             complement(12784..13719)
                     /locus_tag="ACG8WP_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9028317.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3848 a         3106 c         2452 g         4491 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgta
//
LOCUS       DAZKAY010000072        13806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000072 DAZKAY010000000
VERSION     DAZKAY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.073"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..424
                     /gene="yaaA"
                     /locus_tag="ACG8WP_002030"
     CDS             182..424
                     /gene="yaaA"
                     /locus_tag="ACG8WP_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9028318.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            411..1535
                     /gene="recF"
                     /locus_tag="ACG8WP_002031"
     CDS             411..1535
                     /gene="recF"
                     /locus_tag="ACG8WP_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9028319.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1532..3478
                     /gene="gyrB"
                     /locus_tag="ACG8WP_002032"
     CDS             1532..3478
                     /gene="gyrB"
                     /locus_tag="ACG8WP_002032"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9028320.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3498..5969
                     /gene="gyrA"
                     /locus_tag="ACG8WP_002033"
     CDS             3498..5969
                     /gene="gyrA"
                     /locus_tag="ACG8WP_002033"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9028321.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6205..6504
                     /gene="rpsF"
                     /locus_tag="ACG8WP_002034"
     CDS             6205..6504
                     /gene="rpsF"
                     /locus_tag="ACG8WP_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9028322.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6554..7066
                     /gene="ssb"
                     /locus_tag="ACG8WP_002035"
     CDS             6554..7066
                     /gene="ssb"
                     /locus_tag="ACG8WP_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9028323.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7092..7328
                     /gene="rpsR"
                     /locus_tag="ACG8WP_002036"
     CDS             7092..7328
                     /gene="rpsR"
                     /locus_tag="ACG8WP_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9028324.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7471..9444
                     /locus_tag="ACG8WP_002037"
     CDS             7471..9444
                     /locus_tag="ACG8WP_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9028325.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9450..9902
                     /gene="rplI"
                     /locus_tag="ACG8WP_002038"
     CDS             9450..9902
                     /gene="rplI"
                     /locus_tag="ACG8WP_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9028326.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10176..11543
                     /gene="dnaB"
                     /locus_tag="ACG8WP_002039"
     CDS             10176..11543
                     /gene="dnaB"
                     /locus_tag="ACG8WP_002039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9028327.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12323..13615
                     /locus_tag="ACG8WP_002040"
     CDS             12323..13615
                     /locus_tag="ACG8WP_002040"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9028328.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4600 a         2486 c         3029 g         3691 t
ORIGIN      
        1 aaataaaatt ttaaacagaa ggccctaaaa tctcttttaa ggctttctgt ttatttttgt
       61 ttaaatcacc ttttttagat taaatcgtct tatatggaaa aatatatgga taaatttgtt
      121 ttattgcgaa aaaaaaggta taatagagta tacttttaat aatgaaaatg agggatgggc
      181 attgaaacaa caagtcattc taaatacgga attcatgacg ttgggccaaa tgttgaaaga
      241 agtcacagtt atcggtagtg gcggacaagc aaaatggtat ttggcagaaa acactgtgtt
      301 agtcgatggc gaaccagaaa atcgtcgtgg acgcaaactt tatcccggca tgatggttga
      361 agttccagaa gtaggaactt tttttatggt aaaaaaagga aacgacgata atgaggctga
      421 ataagctgta tttaaaaaat tatcgaaatt acgaagagct gaacatcgaa ttttccaaga
      481 acctcgtcat ctttttagga gaaaatgcgc aaggaaaaac gaatcttttg gaaagtatct
      541 acgtattagc tatgacgcga agtcatcgga caagtagtga gcaggaattg attggctgga
      601 ctgacgatca ggcgatgatt caaggagaga tcacaaaagg ttcatccact cttccattag
      661 aaatcttatt atctaaaaaa ggccgaaaaa ccaagatcaa ccatatcgaa caaaaaaagt
      721 taagtactta cgtgggacaa atgaatgtca ttttatttgc accagaagat ctttctttag
      781 taaaaggcag cccgcagatc aggcggaagt ttttagatat ggaattaggc cagatcaatc
      841 caatctatct ttatgatctt gtccaatacc aagcaatttt gaaacaaaga aaccagtatt
      901 taaagcaatt gaatgaaaaa aaacaaactg atttgcttta tttggatatt ttgtccgaac
      961 aactagcagc gtttggcagc aaagtattga aagcaagagc acagttcgtc aagcgattgg
     1021 agttttgggc taattctttg catcagcaaa tcacccatca aaaagagcag ctggaaatcg
     1081 agtatctcac cgcagtcgat tctttagaaa cacatacgca agagcaaatc caagaacaat
     1141 ttctcgcact tttgaaccaa aataaaaaga aagacctttt tcggggaaca acgacagttg
     1201 gtcctcatcg tgacgatctt tcctttttta ttaatcagaa aaatgtgcag acttatggat
     1261 cgcaaggaca gcaaagaaca actgcactaa gtgtcaaatt agcagaaatc gatttgatta
     1321 aagaagagac gggggaatat ccaatccttt tgttagatga tgtgatgagc gagctggatg
     1381 atagtcgtca actccatctg ctagaaacaa tcgaaggaaa agttcaaacg ttcttaacaa
     1441 caacgacact tgaccatgta aaaaataaaa tgactgttga acctgagatt ttttatgttc
     1501 aacaagggaa actaaaagga gaagaaactg aatgacagaa gaaagaagtt tagtagaacg
     1561 cgctaaagag tatgatgcca gtcagattca agtacttgaa ggacttgaag ccgttcgtaa
     1621 acgacctggc atgtatatcg gttcgactag ttctgagggt ctgcatcacc ttgtttggga
     1681 aattgtcgat aattcgatcg atgaagtatt agcaggtttt gcaacgaaaa tccatgtgat
     1741 tattgaaaaa gacaacagta tcaccgttat tgacgacggt cgggggatac ctgtagatat
     1801 ccaggcaaaa actggacgtc ctgcagttga aacggtcttt actgttttac atgctggagg
     1861 aaaattcggc ggtggtgggt acaaagtttc tggtggactt cacggagtag gatcttctgt
     1921 tgttaacgcg ctttctacac agcttgatgt caaagtatac aaagaaggaa atgtgtacta
     1981 tcaagaatat cgtcgtggag ctgtagtgga cgacttaaag attatcgaac aaacggatcg
     2041 ccatggaaca acggttcact ttacgccaga ccctgaaatt tttacagaga caactgagtt
     2101 tgatttttca aaattggcta cccggatccg tgaacttgct ttcttgaacc gcgggatgcg
     2161 tatatcgatt gaagacaaac gagaagaaga accagtcatc aatgaatacc attatgacgg
     2221 cgggattaaa agttatgtcg aatacttgaa tgcaaacaaa accgtgattt ttccagaacc
     2281 tgtatatttg gaaggtgaac agcaagatat cgcagttgaa gtctctatgc aatatacaga
     2341 cggctaccat tccaatatca tgagttttgc caacaatatc catacgtatg aaggcggaac
     2401 acatgaatca ggattcaaga ctgctttaac tcgtgtgata aatgattatg cccgaaaaca
     2461 aaagctgatg aaagaaaatg acgacaactt gaccggtgaa gacgtgcgag aaggattgac
     2521 agctgttatt tctatcaagc atcctgaccc tcaattcgaa ggacaaacaa aaacaaaatt
     2581 gggcaattcc gaagtacgga cagtcactga tcggttattt agcgaacatt tcatgaaatt
     2641 cttgctggaa aacccaagtg tgggccgtca aattgttgaa aaaggattac tagcttccag
     2701 agctcgtcta gcagcgaaac gtgcgcgtga agtaacgaga cgtaaaggtg ctttagagat
     2761 tagtaatctg ccaggaaaat tagcggactg ctctagcaac gatcctgaaa aatgcgaatt
     2821 gttcatcgtc gaaggagatt cggccggcgg atcagctaag caaggtcgta atcgagagtt
     2881 tcaagccatc ttgcctattc gtgggaaaat cttgaatgtt gaaaaagcaa gcatggacaa
     2941 gatcttggca aatgaagaga tccggtcttt atttactgct atgggtacag ggtttggaga
     3001 agattttgat gtatcgaaag ctcgctacca caaattagtt atcatgactg atgccgatgt
     3061 cgacggcgcg catatccgta cattgctgct gactcttttt tatcgttaca tgcgtcctat
     3121 tgttgaagct ggatacgtct atattgctca gccaccttta tacggggtaa aacaagggaa
     3181 gaatattacg tatgtacaac caggaaaaaa tgcagaagaa gagttgaaac aagtcgttgc
     3241 ttcacttcct gcctcaccaa aaccaagcgt tcagcgttat aaaggacttg gagaaatgga
     3301 tgatcatcaa ctatgggaaa caactatgga cccaagcaat cggatgatgg cccgcgtcag
     3361 tgtggacgat gctattgcag cagatcagat atttgaaatg ttgatggggg atcgagtaga
     3421 accccgtcgt gcattcatcg aggaaaatgc acactatgtg aagaacttag atatttaatc
     3481 atgaaggagg aaaaagaatg agtgaagaaa tcagagaaaa catccatgat gtcaatctaa
     3541 ccagcgaaat gaaagactcc ttcatcgatt atgcgatgag tgttattgtc gcacgtgcac
     3601 ttcctgatgt cagagatggt ctgaaacccg ttcatcgccg tattttatac ggcatgaacg
     3661 aattaggtgt aacaccggat aaagcccata aaaaatctgc cagaatcgtc ggagatgtca
     3721 tgggtaaata ccatcctcat ggagactatg cgatctatga gtcaatggta cggatggcac
     3781 agccattcag ctaccgttat atgctagtgg atggtcatgg aaactttggt tctgttgacg
     3841 gtgacggtgc cgccgctatg cgttataccg aagcacggat gagcaaaatc gctacagaaa
     3901 tgctgcgtga tatcaataag aacactgttg atttccaaag caactatgat gatacagaaa
     3961 aagaaccagt tgtactacct gctcgcttcc cgaatctttt agtgaacgga acaacgggga
     4021 tcgccgtagg tatggccacg aatatcccac ctcataactt gtctgaggta gtagcggcta
     4081 tcgatttgct gatggataat cctgatgtga cgaccaatga attgatggaa gtattacctg
     4141 gaccagattt tccaacaggt ggtttggtta tgggaaaatc aggaatccga agagcgtatg
     4201 aaacaggtaa gggttctatt accgtacgtg caaaagttga gattactgag atgccaaatg
     4261 gtaaagaacg gatcatcgtg acagaattgc cttatatggt caataaagca aaattgatcg
     4321 aacggatttc tgaactgcac agagacaagc gtatcgaagg gatcacagat ttgcgggacg
     4381 aatcttcccg tgaaggtatg cggatcgtca ttgacatccg tcgtgacgcc agcgcatctg
     4441 ttatcttgaa taacttatat aaattgacat ccttgcaaaa ttcatttgga ttcaatatgt
     4501 tggcgatcga aaaaggtgtg ccgaaagttt tgagcttgaa acaaattctt gaaaactata
     4561 tcgaacatca gcgcgaagtc atcacaagac ggacagtctt tgaaaaagaa aaagcagaag
     4621 caagggcaca cattttagaa ggtttgcgta ttgctttgga tcatatcgat gaaatcattg
     4681 cgattatccg cgggtctaaa tcagatgacg aagcaaaagc gacgatgatc gaacggttcg
     4741 atttatccga tcgccagtct caagctattt tagatatgcg tttacgccga ttgaccggtt
     4801 tggaacggga aaaaatcgaa aatgaatacc aagaactctt aaaactgatt gaagatttaa
     4861 ctgacattct ggcaagacca gaacgtgtta cagaaatcat caagaatgaa ttagcagact
     4921 tgaatcaacg atttggtgat aaacgacgca cagaattact agtcggtgag gtcttaagtc
     4981 tggaagatga agatttaatc gaagaagaag aaatcgttat tacattgaca aacaatggtt
     5041 acatcaaacg tttggcaaat aatgaattca gagctcaacg aaggggtgga cgtggtgtcc
     5101 aaggaatggg tatccacaat gacgacttcg tcaaaaatct ggtttcttgt tctacccatg
     5161 atacattgtt attctttacg aataacggaa aagtgtatcg ggcaaaagga tacgaaattc
     5221 cagaatacgg gcgcacagcc aaaggaatac caattatcaa tttactaggt atcgattcga
     5281 gtgaaagcat ccaggcaatc attgcggtga cgggagaagc agaagaaggc cactatctct
     5341 tctttacgac gcgtcaaggt acagtaaaac gtacttctgt cactgctttt gctaacatcc
     5401 gaagtaatgg tctgatcgca attggtttga aagaagacga tgagctcgtc aatgtgctac
     5461 tgacagatgg tcaatctaat atcattattg gaacacataa cggctactct gtcactttca
     5521 aagaagaagc agtccgcgat atgggacgta cagcttccgg ggttagagga atacgcctgc
     5581 gtgaggaaga ttatgtcgtt ggggcagcct taatgaagga agatcaagaa gtattagtga
     5641 tcacagaaaa aggttacgga aaacggacaa aagtatcgga atatccagtg aaaggccgcg
     5701 gaggtaaagg gatcaaaacg gcgaacatca ctgaaaaaaa tggtccacta gctggattga
     5761 ccactgtttc tggagatgaa gacatcatgc tgattacaga taaaggtgtc attatccgtt
     5821 tcaacgtatc gactgtttct caaactggac gttcaacatt aggcgtacgt ttgatgaaga
     5881 tggaagctga tacgaaagtt gtcacgatgg cagcagtcga accagaagct gcagaacaaa
     5941 cagttgaaga acaaggatca gaagaatagc catcaagact aattgtgtaa gctagcaaga
     6001 atgaccgatt aaaaaatctg gttattcctg ctggcttttt tgattagttg aatttctctt
     6061 aaaatattcg aaactgttca ttctgtattg cttttgacca aaatagagtg tataatcaga
     6121 gcttgtgagt agtctaatta attagataac tctccttgct cttaggaacc taagagccga
     6181 aagtccacag ggaggtgaat atcaatggaa aacacgaaat atgaaatcat gtacattatt
     6241 cgtccaaaca ttgatgaaga agcaaaaact gctttaatcg aacgtttcga tgcaatcttg
     6301 aaagataacg gagcagaagt tatcgaatca aaagattggg aaaaacgccg tttcgcttat
     6361 gaaatgaacg gttaccgtga aggtatctat cacatcgtta aagtatcttc tccatcatca
     6421 gcaaatgctg tcaatgaatt tgaccgtctt gctaaaatca acgacgatat cattcgtcac
     6481 atgattgtta aagaagaagc ttaattgttg tttcatgtga aacaatagaa aacgatgaga
     6541 ggagatgact attttgatta ataatgttgt attagttgga cgtctgacga aagatccaga
     6601 tttgcgttac acagcaagtg gtacggcagt cgcaacattt actttggctg tgaatcgtaa
     6661 ttttacaaac caaagcggca atcgcgaggc tgattttatc aactgtgtga tttggcgcaa
     6721 atcagcagaa actttagcaa actatgctcg taaaggaaca cttttaggtg ttactggacg
     6781 tatccaaact cgttcttatg ataatcagca aggacaacgt gtatacgtga cagaagtagt
     6841 agctgacaac ttccaattgc tggaaagtcg ttctgcatct gaaaatcgcc aacaaagcgg
     6901 cggattccaa agttctggtc aatcagctgg cggatttggc ggaaacaata actctaatca
     6961 aacatctcaa tcatccaacg gtatgcctga tttcgaccgt gatacttctg acccattcgg
     7021 ctcaagttca acgatcgaca tttcagacga tgatttacca ttctaaacga atttgatagg
     7081 agggaaatag aatggcacaa caaagaagag gcggacgtaa acgtcgtaaa gtagactaca
     7141 ttgctgctaa ccatattgaa tatatcgatt ataaagatat cgaattacta aaaagattta
     7201 tttcagaacg cggtaaaatc ttgccacgtc gtgtaactgg tacaggcgct aaaaaccaac
     7261 gtaaaattac tgttgcaatc aaacgcgctc gtatcatggg cttgctacct ttcgttagcg
     7321 aagactaata aatcctagga attgaatgac tgatcctttt gatcagctat tcaattcctt
     7381 tttttctgta aaaataacct ctattcgttt agtcgctcga atgacgaaag ttatggtaaa
     7441 atcaaattag tcatatatgg gaggaaaatt atggaaaata aaaacaatca tttatccccg
     7501 aaacctctct tccttatcct gtttatatta atcgaagtat tggtagtctt aattttacca
     7561 tttcgttttg ttagcgcttc ggtcattcta ttgatgaatt tatggggtgt tcgctattgg
     7621 ttgaaaatga aacgttggct tattttaaat aaacgtgaaa aaatccagca ggcttcgact
     7681 gtagctgaaa ccaatctagc ttatgtctca gaagtcatgc ctgtaggagt gattgcttat
     7741 cagccatcaa gccaaaaaat cgaatggctc aatccattag cattgaaaat caaagatcaa
     7801 agtggattaa cagaggaaga attgctagat acttttttta ctgcaaaaga aaaaaagaaa
     7861 aaccaagtct atctagcaga taaaacattt cagtttgata tggatacagt aaagggtgtt
     7921 gtttatttta atgatattac ccaaaaccag ttgttaaaac tccatcagac gaacagtcag
     7981 ccggtgattg gtattttatc tattgataac tatgcagatg ccattgataa attggatgac
     8041 aaagagattt cttatttgaa ctctttcgtc actacactag tttctgactg gatgaatgaa
     8101 taccatgtct ttttcaaaag aatcaacgca gaacggtttt tcttcgtagc acagatgatc
     8161 gatctgaaaa agatgatgga taaaaaattt gatttgctgg atcgctttcg agcagaagct
     8221 gaaaaacaga gctcgcctat cacccttagt atggggattg cttatggctc aagaaccctc
     8281 gatgaaatcg gtggagaagc tcagaataat ttagatgttg ctttagtccg tggaggcgac
     8341 caagttgtcg tgaaagaggt aaaagaagga gcaaaacctg tttattatgg ggggaaatct
     8401 gctaagaatg tcaaacgcac gcgtgttcgt tctcgagcga tgagtaccgc tttgcgtcgg
     8461 atttttgatg aaacaggcga tgtgtttatc atggggcaca agtttccgga tatggatgct
     8521 ttaggctctg cttttggtgt cagttgtctg gcacgcttca accagaaaaa agcttacatc
     8581 gttatcaacg agaacgagat cattccagat gtagaacggt gtcttgaaga aatccataag
     8641 catcctgaat tagaaatgca actaatctct ccagaacgtg ccatagagct gaaaaaagaa
     8701 gaggacctcc tcgtgatggt ggattaccat aaaccttctc tatcgatctc tcagggccta
     8761 tatgagcaat ttgataagat tgtgatcatt gatcaccatc gtcgtgggga agagtttcca
     8821 agtaaacctc tgctgactta tatcgaatct tcagcttctt cagcttcaga gctagtttct
     8881 gagctgatcc aataccaaag cagcaaaaca aatcgcttag atcgttttga ggccacatta
     8941 ttgcttgcag ggatggttgt agatacaaat agtttcaatg tccgtacaac tgcacgaact
     9001 tttgacgttg ccagctattt aaggtcttgt ggagcagacg catccttagt acaatattta
     9061 ttgagctcgg atttagcgac atacttggag atcagcaatt tgattgctgg aagcgactat
     9121 gtgacttctg atattgttgt ggcggcaggg acagacgaaa aagagtataa tagtgttata
     9181 gcagcaaaaa cagcagatac attgctttcg atgatcaata tcaatgcagc tttcgtcatt
     9241 acaaagcgga cagacggctt gattgggatc agtgccagaa gtaccggcac gatcaacgtc
     9301 caaatcatca tggaatcctt aggtggcgga ggacacttca caaacgcagc tactcaactg
     9361 gaaaatacga cagttgagga aacaaaacag aagctatttg ctgcaatcaa agaaaatatg
     9421 aatcaaatat atgataagga gtaatccaaa tgaaagtaat ttttttacaa gatgtaaaag
     9481 gaaaaggtaa aaaaggggac atcaaagaag ttccagcagg ttatgcacaa aattatctaa
     9541 tcaaaaataa cttggcaaaa gaagcaacga aaagcagtat cagtcagtta gcaggacaaa
     9601 agaaagcaca agaaaaacac gaagctgaaa tcttggaaga agctaaaaag ttaaaagaat
     9661 tcttggaaaa agaagatact gtagtcgaat tgaaggcgaa agcaggggaa gacagccgtt
     9721 tgtttggttc gatcccttca aaacaaattg ctgaagggtt aaacaaacaa tacaaaatca
     9781 aattagataa acgaaaaatc gaattagaaa atccgatccg ttcactaggc tacacaaacg
     9841 tacctgttaa gcttcatcat gaggtgacgg ctacgatcaa agttcatgtc gtacaagaat
     9901 aacaaatctt gatagcgatg ttcagctggt aagtataacc agaaggaaaa aagacaaacc
     9961 gaaatttatc tgagaaaaca gctgcagcaa tgaaagcaaa agctgcaaat cgatttcaga
    10021 ttgatttcgg tttctttttt gggaacagca aaatagcata aagaaaacga gtataataaa
    10081 agagaactat ttttttatta cactttcggt acgaatgatg acttttctct cattttcttg
    10141 taaaatgaag caattgaaga caaggagcga tacgtatgaa tgaagtgtgg caggatcgaa
    10201 tcccaccgca aaatattgaa gctgaacaag cggttttagg ctctgttttt cttgacgcag
    10261 aaacgatcat tgatgccatg gagtacatag agccaaaaga cttttatcgt cgtggccatc
    10321 aaattatttt tgagacaatg atcgagttaa atgatcgtaa tgaagcgatt gacgtggtta
    10381 ctgtcaaaga ccggctggaa caagccaatt tgttagaaga tgccggtggt ttaagttatc
    10441 tctcagactt ggcattagca gttccgacag cagcaaatat cgtctactat gcgaaaatcg
    10501 ttgaagaaaa atcattgctg cgtacattga tccagacagc aacaggaatc gttacgaaag
    10561 gattcgagca aggagaagaa gttcaaacaa ttttagatga tgccgaacgc agtatcttgg
    10621 aggtttcgga aaaaagaaat cgaagcggct ttttatctat tgctgaagta ctgaactcca
    10681 cgattgaaaa tatcgatcga ctagctcaaa acaatgaaga aatcacaggt ctaccaactg
    10741 gctatcaagg tctggataaa atgacagcag gtttccagcc cgaagaacta atcattcttg
    10801 ctgcacgtcc agcggtaggt aaaacagcgt ttgcattgaa tattgctcaa aatgtaggaa
    10861 caaaaacaga tcgttctgtt gcgatattta gtttggaaat gggggcagaa tctcttgtaa
    10921 atcggatgct ttgtgcagaa ggttcgattg aggccagcca tttacgtaca ggtcagcttt
    10981 cggaagaaga atggtcgaat ctgatcgttg cgatggggag tctttctcga gcaaatattt
    11041 ttatcgatga tacaccaggg atcaaaatct ctgaaatccg tgcgaaatgt cggaaactag
    11101 cgcaagaaaa aggcaatcta ggcttgatat tgatcgatta tttgcagttg attgaaggta
    11161 acggtaaaga gaaccgtcaa caagaagtat cagatatctc tcgccaactg aaaaaactgg
    11221 cgaaagagct gaaagtacct gtcattgctc tttcacagtt atctcgtgga gtagaacagc
    11281 gacaagacaa acgaccagtg ctaagtgata ttcgtgaatc cggatcaatt gaacaagatg
    11341 cggacatcgt agccttcttg tatcgagatg attattatga aagagaaggc ggagatgacg
    11401 atgatggaaa cgagccaaaa aatgacaacg tcatcgaagt catcatcgag aagaaccgaa
    11461 gcggtgcacg aggaacagtt gaactactgt ttatcaaaga atacaataag ttttcgtcgc
    11521 tttcgccaag agaagaattt taaagaagag gttgtgagcc ttgagcaatc ccgagagctc
    11581 tcgtaagaag atgcaaggag taaccgcagg aacgactaga gtacttaatt taaacagatc
    11641 tccaaaggga ttgtgacaaa agtcttgtca cagtcccttt ttccgaataa acggtgttca
    11701 atcagctgac ccttggtatt agctcagcaa acgtcaaaat atgaggaact attttgacgt
    11761 ttgtttctct aatactcagg tcaaaacgct gatcttacga cctcttttcc ttaaacggtg
    11821 ttctcctgcc aactcctcgg tcacaagtca caatatcatc aaacatgaga agcagttttt
    11881 tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctt tcttaaacgg
    11941 tgttcaaaag cttgcatctg cggtaatacg cccaatcaaa cgaaaaatat gagaagctat
    12001 ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacaacc tctgattcac
    12061 ggtgttccaa aagtagcttc ttcgaaaaat aagtcgaaat ttcacaaaaa tttgagaagc
    12121 aattttcgta cattccttct tatttcttga agctgaccac ttctgttaca acctcttttt
    12181 taataaccat tctaaaaaga taaagttcgt tttttaagcg tttttataaa ataaaaataa
    12241 aaatcattcg attttatttg aaatatgtat ctattattga tacaatggtt caggattaaa
    12301 taatatagaa cgaggtgttc acatgtcatc tgtagtagta gtaggtacgc aatggggcga
    12361 tgaagggaaa ggaaaaatca cagactttct cagtgaaaat gctgaagtga ttgcacggta
    12421 ccaaggtggc gacaatgcag ggcacacaat taagtttaac ggtgtgactt ataaactgca
    12481 tttgattcct tctgggattt tttataaaga caagatcagt gtgatcggaa acggcgttgt
    12541 agtcaatcct aaatcattgg ttaaagaatt agcttattta catgaaaaag gtgtagaaac
    12601 aagtaatttg cgtatttctg accgcgcgca cgtaatcttg ccttatcata tcaaattgga
    12661 ccaattacaa gaagatgcta agggcgaaaa taagattggt acaacgatta aaggaatcgg
    12721 acctgcctat atggataaag cagctcgtgt aggtatccgt attgcagact tattagataa
    12781 agaaattttt gaagaacgtc ttcgtttgaa tctagatgac aaaaaccgtc aatttgtaaa
    12841 aatgtttgat tcagaaccac ttgattttga cgatattttt gaagaatatt atgaatacgg
    12901 acaacaaatc aaacaatacg tcacagatac ttccgttatc ttgaatgatg ctttggatgc
    12961 aggcaaacgc gtgttgtttg aaggcgcaca aggtgtaatg ctggatatcg accagggaac
    13021 ttatcctttt gttacttctt ctaacccagt agccggtggg gtaacaatcg gaagcggtgt
    13081 agggccttca aaaatcaata aagttgtcgg cgtctgcaaa gcctatacgt cacgtgtcgg
    13141 tgatggacca tttccaactg aattatttga tgaaacagga gaacaaatcc gcgaagtagg
    13201 acgagaatac ggtacgacaa ctggtcgtcc gcgtcgtgta ggctggtttg attctgtagt
    13261 tatgcgccat tctaaacgag tttcaggtat taccaaccta tctttaaact cgatcgatgt
    13321 attaagtgga ttggaaacag taaaaatttg tacggcttat gaattagatg gcgaactaat
    13381 ctaccactat ccagcaagct tgaaagagtt gaaccgctgc aaaccaatct atgaagaatt
    13441 gccaggctgg tcagaagaca ttacaggatg caaaacactt gcagaactgc cagaaaacgc
    13501 acgtaactat gtacgtcgta tctcagagct tgttggtgta cgtatctcaa cattttctgt
    13561 cggacctgat agaacacaaa caaacatctt agaaagcgtc tgggcgcaaa tctaaggata
    13621 aaaacgacga ctgaatcagg acgttcttac cagagcgagt cgaagtgttt atctatacga
    13681 taataataac ttgaagaggt agtgaaagag acgtttagcc ttattgccga taggctgtct
    13741 cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac agtccctttt
    13801 tccgaa
//
LOCUS       DAZKAY010000073        13787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000073 DAZKAY010000000
VERSION     DAZKAY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.074"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1617
                     /locus_tag="ACG8WP_002041"
     CDS             190..1617
                     /locus_tag="ACG8WP_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028329.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1709..2074)
                     /locus_tag="ACG8WP_002042"
     CDS             complement(1709..2074)
                     /locus_tag="ACG8WP_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9028330.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2071..2436)
                     /locus_tag="ACG8WP_002043"
     CDS             complement(2071..2436)
                     /locus_tag="ACG8WP_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9028331.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2628..2876)
                     /locus_tag="ACG8WP_002044"
     CDS             complement(2628..2876)
                     /locus_tag="ACG8WP_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028332.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2875..3003
                     /locus_tag="ACG8WP_002045"
     CDS             2875..3003
                     /locus_tag="ACG8WP_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9028333.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3060..4754
                     /locus_tag="ACG8WP_002046"
     CDS             3060..4754
                     /locus_tag="ACG8WP_002046"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9028334.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4821..5150
                     /locus_tag="ACG8WP_002047"
     CDS             4821..5150
                     /locus_tag="ACG8WP_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028335.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5528..6661
                     /locus_tag="ACG8WP_002048"
     CDS             5528..6661
                     /locus_tag="ACG8WP_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9028336.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6692..7012
                     /locus_tag="ACG8WP_002049"
     CDS             6692..7012
                     /locus_tag="ACG8WP_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9028337.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(7041..7505)
                     /locus_tag="ACG8WP_002050"
     CDS             complement(7041..7505)
                     /locus_tag="ACG8WP_002050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028338.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7636..8889)
                     /locus_tag="ACG8WP_002051"
     CDS             complement(7636..8889)
                     /locus_tag="ACG8WP_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9028339.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8918..9235)
                     /locus_tag="ACG8WP_002052"
     CDS             complement(8918..9235)
                     /locus_tag="ACG8WP_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9028340.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9261..9581)
                     /locus_tag="ACG8WP_002053"
     CDS             complement(9261..9581)
                     /locus_tag="ACG8WP_002053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9028341.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(10045..11460)
                     /locus_tag="ACG8WP_002054"
     CDS             complement(10045..11460)
                     /locus_tag="ACG8WP_002054"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9028342.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11477..12793)
                     /locus_tag="ACG8WP_002055"
     CDS             complement(11477..12793)
                     /locus_tag="ACG8WP_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9028343.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12943..13641)
                     /locus_tag="ACG8WP_002056"
     CDS             complement(12943..13641)
                     /locus_tag="ACG8WP_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9028344.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4381 a         2791 c         2363 g         4252 t
ORIGIN      
        1 tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt tttccaaatt
       61 ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg
      121 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
      181 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      241 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      301 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      361 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      421 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      481 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      541 aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc
      601 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      661 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      721 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      781 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      841 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      901 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      961 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
     1021 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
     1081 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1141 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1201 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1261 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1321 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1381 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1441 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1501 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1561 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1621 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1681 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct
     1741 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1801 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1861 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt
     1921 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1981 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     2041 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     2101 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2161 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2221 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2281 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2341 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2401 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2461 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2521 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2581 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2641 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2701 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2761 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2821 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2881 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2941 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     3001 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     3061 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3121 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3181 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3241 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3301 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3361 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3421 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3481 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3541 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3601 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3661 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3721 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3781 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3841 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3901 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3961 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     4021 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     4081 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4141 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4201 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc
     4261 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4321 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4381 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4441 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4501 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4561 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4621 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4681 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4741 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4801 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4861 cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     4921 cacccaagct aaaactgctt cttatgtttt tgacttggtt ggacttatta ccgcagatgc
     4981 aagcttttga acaccattta ccaagaggtt atgaaaaagc tgccctgcat caagtaataa
     5041 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta ttaccgcaga
     5101 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag tttcgagtat
     5161 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
     5221 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
     5281 ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat acaactattt
     5341 caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca aatacataag
     5401 actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct
     5461 ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc
     5521 atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga
     5581 tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg
     5641 cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt
     5701 aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa
     5761 agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa
     5821 tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg
     5881 atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta
     5941 tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt
     6001 tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg
     6061 cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt
     6121 tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc
     6181 ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga
     6241 attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg
     6301 attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc
     6361 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
     6421 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
     6481 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa tgattgcaga
     6541 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
     6601 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
     6661 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
     6721 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt
     6781 ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat
     6841 ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt
     6901 ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat
     6961 ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagaattttt
     7021 caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc
     7081 ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta
     7141 tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc
     7201 gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa
     7261 agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc cgctcctccg
     7321 acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg
     7381 attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac
     7441 caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca gttatatgta
     7501 accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg
     7561 aaaattagca atgacaacaa tagtgatgta aaaaaaaaac ggaaaacaat cacttgttct
     7621 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7681 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7741 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7801 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7861 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7921 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7981 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     8041 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     8101 gctatgccca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     8161 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     8221 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8281 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8341 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8401 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8461 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8521 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8581 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8641 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8701 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8761 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8821 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8881 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8941 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     9001 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     9061 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     9121 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     9181 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9241 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9301 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9361 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9421 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9481 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9541 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9601 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9661 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9721 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9781 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9841 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9901 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9961 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
    10021 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
    10081 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
    10141 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
    10201 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10261 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10321 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10381 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10441 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10501 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10561 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10621 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10681 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10741 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10801 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10861 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10921 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10981 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    11041 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    11101 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    11161 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    11221 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11281 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11341 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11401 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11461 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11521 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11581 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11641 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11701 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11761 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11821 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11881 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11941 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    12001 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    12061 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    12121 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    12181 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12241 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12301 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12361 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12421 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12481 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12541 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12601 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12661 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12721 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12781 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12841 gcttacattt agatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12901 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12961 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    13021 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    13081 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    13141 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    13201 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13261 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13321 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13381 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13441 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13501 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13561 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13621 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13681 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13741 catagacata caaacgaaga agatatttac cataaaaaag actagga
//
LOCUS       DAZKAY010000074        13759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000074 DAZKAY010000000
VERSION     DAZKAY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.075"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8WP_002057"
     tRNA            2..74
                     /locus_tag="ACG8WP_002057"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8WP_002058"
     tRNA            78..150
                     /locus_tag="ACG8WP_002058"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8WP_002059"
     tRNA            155..225
                     /locus_tag="ACG8WP_002059"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8WP_002060"
     tRNA            238..311
                     /locus_tag="ACG8WP_002060"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8WP_002061"
     tRNA            323..394
                     /locus_tag="ACG8WP_002061"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8WP_002062"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8WP_002062"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9028345.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8WP_002063"
     CDS             1983..3455
                     /locus_tag="ACG8WP_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9028346.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8WP_002064"
     CDS             3538..4326
                     /locus_tag="ACG8WP_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9028347.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8WP_002065"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8WP_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9028348.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8WP_002066"
     CDS             5176..6888
                     /locus_tag="ACG8WP_002066"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028349.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACG8WP_002067"
     CDS             6891..8660
                     /locus_tag="ACG8WP_002067"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028350.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACG8WP_002068"
     CDS             complement(8865..9431)
                     /locus_tag="ACG8WP_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9028351.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACG8WP_002069"
     CDS             complement(9431..10387)
                     /locus_tag="ACG8WP_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9028352.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACG8WP_002070"
     CDS             complement(10387..11043)
                     /locus_tag="ACG8WP_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9028353.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACG8WP_002071"
     CDS             11413..13431
                     /locus_tag="ACG8WP_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9028354.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4260 a         2487 c         2930 g         4082 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaac
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaa
//
LOCUS       DAZKAY010000075        13502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000075 DAZKAY010000000
VERSION     DAZKAY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.076"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..322
                     /locus_tag="ACG8WP_002072"
     CDS             155..322
                     /locus_tag="ACG8WP_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9028355.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(384..2195)
                     /locus_tag="ACG8WP_002073"
     CDS             complement(384..2195)
                     /locus_tag="ACG8WP_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9028356.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2192..2557)
                     /locus_tag="ACG8WP_002074"
     CDS             complement(2192..2557)
                     /locus_tag="ACG8WP_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9028357.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2720..3115)
                     /locus_tag="ACG8WP_002075"
     CDS             complement(2720..3115)
                     /locus_tag="ACG8WP_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9028358.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3133..4095)
                     /locus_tag="ACG8WP_002076"
     CDS             complement(3133..4095)
                     /locus_tag="ACG8WP_002076"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9028359.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4095..4307)
                     /locus_tag="ACG8WP_002077"
     CDS             complement(4095..4307)
                     /locus_tag="ACG8WP_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9028360.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4328..5026)
                     /locus_tag="ACG8WP_002078"
     CDS             complement(4328..5026)
                     /locus_tag="ACG8WP_002078"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9028361.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5046..5588)
                     /locus_tag="ACG8WP_002079"
     CDS             complement(5046..5588)
                     /locus_tag="ACG8WP_002079"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9028362.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5720..6724)
                     /locus_tag="ACG8WP_002080"
     CDS             complement(5720..6724)
                     /locus_tag="ACG8WP_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9028363.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6721..7710)
                     /locus_tag="ACG8WP_002081"
     CDS             complement(6721..7710)
                     /locus_tag="ACG8WP_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9028364.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7707..8513)
                     /locus_tag="ACG8WP_002082"
     CDS             complement(7707..8513)
                     /locus_tag="ACG8WP_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028365.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8679..9635
                     /locus_tag="ACG8WP_002083"
     CDS             8679..9635
                     /locus_tag="ACG8WP_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9028366.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9712..10230)
                     /locus_tag="ACG8WP_002084"
     CDS             complement(9712..10230)
                     /locus_tag="ACG8WP_002084"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9028367.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10318..10467)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_002085"
     CDS             complement(10318..10467)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9028368.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10695..11141
                     /locus_tag="ACG8WP_002086"
     CDS             10695..11141
                     /locus_tag="ACG8WP_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9028369.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11336..13231)
                     /gene="asnB"
                     /locus_tag="ACG8WP_002087"
     CDS             complement(11336..13231)
                     /gene="asnB"
                     /locus_tag="ACG8WP_002087"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9028370.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4217 a         2848 c         2295 g         4142 t
ORIGIN      
        1 aaatttcgta aattcatatg gtaaatttat aataaaacat ttagtaaaca ttttcaaggg
       61 ttttttgcaa aggtttacaa acaaacgtaa aactagcctt tttttctctt ttgttccata
      121 atagatataa caaataataa atgaggtgga ttcaatgaaa aaatttgtat ctggaatatt
      181 agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac
      241 agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat
      301 gcgtaaacgc gtttctcgtt aataattatt taaaaatacg catgaagact ttctaaaaaa
      361 cagataagtc atcatgcgta tttttatttg tcagtcaata taagttgtgc tattttatct
      421 gcctgtctca gattccatgg atctgtataa ggtctagcta ctgctgcagt tagccaatcc
      481 acaccttcaa caggtattcc atagcaataa aagtcagact ggatttcccc tgaagcatca
      541 atcacgcgat tcgtacctcg ctcaacatct atagcttctg aatgataact tcctgtatta
      601 tctgaaaatg aaaaagggcg aacaagtttg ttttccttca aattttttat gacaggatta
      661 cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt
      721 tctttatctt gtagagaaat aacatagcgg ttgtgctttt gctgaatatc aaatggcggt
      781 tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta attctttcgt ccgaatgatt
      841 ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc atccccatag tatttctttg
      901 tattccttag cagaaaaaac gtgccattca atagcctgcc ggattggatc acgccagtct
      961 tttaatgcat caaatgttga agcaaatgct cctgtgcaat tgcctttttc cgcttctttt
     1021 atctgccctt caataaatat tcggctagct tctgaaaagg gctcattctg ataggcaaga
     1081 ggagtttcta aatacgacca atcccatcta taagggatta gtgctggatg ctttccgaga
     1141 cactcctctg aagagaggga gagaaaatca tgctcaaaat ttttctttgg aatcggcaag
     1201 tgatattctt cgatgatttt cttgtaataa aacaactcaa cgtctttttt cagatagtca
     1261 aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt gctccaggtt ttcttttgtt
     1321 agtaaccgtg gccatgccat tgctccgcct tgcttttgat ttcttcctcg agggtaataa
     1381 gggagccccc ttcttgatcc actgtataca atcggttcct tgcctgatgg atgatagacc
     1441 agttttccct tttcgttctc gaagcgccct cctcttgata ctgtaaacaa tccgacataa
     1501 tcaaaaaaac ttaagcctag acctcttaaa aagacatttt ctttaggcgg tatcttcgaa
     1561 agatctacat ctgcaggatt agaaggaagc tgatacagta aaacattttt ctttgcgtag
     1621 tctacccaca ctctttcttc ttctgtctca tcattttccc agtgaccgct tgctaaaaca
     1681 agctgatcga tagaatagct ttgctgatct gtctgtaaaa gataaccatc ttttcttttg
     1741 cttacttttt ttactaaaga ataaacaaac tgaatagaag ctggaaattc ttcttgcaac
     1801 tttgagaaaa accagcgttg atagagtccg tataaacatc gagtagattg ctgatctttt
     1861 tccagacgat tgatttcttc tataattagt tgcttatttt tggttaccgt ctgttcgata
     1921 taatctgtag caggtccttt actccattga aacaagttgg gtccctgtgt taccaatcct
     1981 ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga attcattaat
     2041 aactccggtg gctgattcaa ccgccaaact cgacctccag gaccattggg atcgaaaaga
     2101 aaaagttcaa gagaatgtct gtcctgatta ttatggagaa tcctttctgc tacacttaat
     2161 cctctaggtc cggcaccaat gatccctatt ttcatttgca tgcctccatt tcgataaatt
     2221 ttttcaataa aacagcatgg ttctgatgtt cattttttgc accataaaca aaagtaatga
     2281 ctggatgttc tttcataagt tgactgagtt taaaaatagc ttgcttcttt tcttcttcga
     2341 aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa
     2401 aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa ccacagatct aactgcgctt
     2461 tttcttttga aattcctcgt ggccagaatc tatccacgag tattctatag ccatcctctt
     2521 tttcagcatc aaggtaaaca cgttttaatt tgatcatagt tgtttccttc tttctacctt
     2581 ttcgcttagc ttatatatgt agataatagc acaaaataaa aaaaatgaac ggcacaacta
     2641 tccattgcgc cgttcatgtg atcttggtca aaatctaatg attgattcgg tttttaaagc
     2701 tgtcatacaa tcatctgttt tatacttttt tacttttgat ttttccagac catccgtcat
     2761 atccaccctt aagtaaataa atctcttgat aacctgcttt acgtaattta tttgctgtac
     2821 ggatgctcaa acttttcttt tgatcgtaaa tatagacagg ctggtctttt cgaatagatc
     2881 ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata tgcccagcat
     2941 caaacacatc tttttcgcga acatcgatta cttgtgcttt tctcattcct tctcgaaatt
     3001 cttcttctgt caaagtcgtt gctgaacgtt tggccattac tcgtagatag acttcccaaa
     3061 ggatgattgc aagcacaatc aatagcaaaa tgatattaat tacccataaa accatgtaat
     3121 gcctccgttt ttttaagcaa atttcaaggc taatgaggca gcaccaatga caccagcttc
     3181 attaccaagc tctgctaatt tgatttttgt tgattctctt acttgcggga aagtaaattc
     3241 tttaaaatat ttctctacac ggctacgtaa aaattctcct gctgccgata ctcctccacc
     3301 taacacgata cttgacggat ttaatgtatt ccccaagttt ccgcaagcta aacctaagta
     3361 gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa
     3421 gatatcttta cttgttactt cttctccgtt gtctagcatt gctttcagtt ttgaatcccc
     3481 tgcgtattct tctgccaaga aacgacctag gcgtaccaca cctgtagcgc tagatacagt
     3541 ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg
     3601 tccgatttct cctccagctc cgctcacgcc gtgaatcaaa tttccgccag caacgatacc
     3661 tccgccaaca cctgttccta gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc
     3721 tttccatcgt tcacctaaag cagcaacatt cgcgtcatta tcgatagcga atttgattcc
     3781 tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct gtccagttta agttatacgc
     3841 accaataacc gttccagctt cgctgtccac gcttcctggg gtacccatgc cgattccgat
     3901 aaagtcttcg gctttcatat tatataaatt caaacgatga ttgattgatt caatgatttc
     3961 aggaacgata tgcttaccat catccaaaat attggtatcg atgctccatt tttgctgaat
     4021 ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat caatcccaat
     4081 aatcttcttg tccattacaa atttcctttc tttcggcttt cttcaattcg gtgctctctt
     4141 tttaatatgc gccacgctgt catgtatgta tcatgatcaa tcaaacggct atcatacaga
     4201 ttacgcaatt cgatagtcat taattcaatg tcatagatgc gtgcacccac atagataaag
     4261 atgccaaatt ttttgaacag ctgctgtaca tcatataagc tctccatctt taagcacttc
     4321 cttcatatta tacaagtaaa ccatattttt ttaaacccag taaaatacat attacaaaaa
     4381 gaaatacaaa aactagtgta gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt
     4441 tcggtagacc caaaacattc cctaacacta atccgccaat tagcccaccg acatgtcccc
     4501 aaacatctac agaggtatcc agaagaccaa aaacaaatgt caacacaata aataaaagaa
     4561 aatgtctgac tagctgctgg atcgctgggt tatgtttaaa atgaaaccct aagataaaca
     4621 acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt
     4681 taaaagcaaa gcttgctgta tttcccatta tcccgctgaa catataaatc aagaaaaaac
     4741 gccaatggcc atagattgcc tcgatttgct gccccataaa atacagtatc agtgagttca
     4801 ttacgaaatg catcagtcca aaatggatga agattggtgt cactaaacgc caccattcat
     4861 tgaagtgaac aagataagga ccatacattc cggcataata agggatctca agtcgcggaa
     4921 ataattccat catgaggaag accgctgtct gtatccctaa aaaagcatag gtccataacg
     4981 gtcgtcttaa ccatgcacgc aattttattt gctgttgata attcatgaaa acctcccatt
     5041 tcattttagt caataaacaa ttgttggatc ttctgatcaa agctctccgg ttgccattgt
     5101 tcattcaatt gttcacgaaa aacaaggcta catgtttttt tatgaaaatt atttaaatac
     5161 cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg ctctattgta gataatacct
     5221 ggtacaatga ggagatcgat catctccggc aaatagagct tctctgattg tggctcaaga
     5281 acaccaaaac tcgaacgtac aagaacggac tgttcatcca tttcataaaa cgccatctga
     5341 cgtttcggca aagttttagg tacaactatt ttcttttgtt ctttttctgc tcgttggata
     5401 accggctgtg tatccagttc aaattcattc gataaagtcg tccctatcac ttttgctgac
     5461 ttccagtact cgctggcgaa tagctgttta tagattcgct gttctttttc ctttttcttg
     5521 ccacttttgg ccagattttt cagcttttcg attcctttcc ggcgtaatct tactttcaat
     5581 tggtccaaaa actcaccctt ccttctctca taaaatggta taggaaaaaa agaaaaatag
     5641 caatcgaact ttcaaataaa tgcataaata gaataaaaag aagacctaga aatgattggc
     5701 cttccttttt taatgaattt caactgttca gcgcgtgttt ttgtctgata aaaagtgcat
     5761 acagaaaata tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact tccccacttg
     5821 gtagaatcac tctagcaacc atatctgcac taagcagaaa aatggaaccg aacaaaccgc
     5881 tcagaacaaa ggtccagtta ttttctttac gtacgactag cttcgcagca tgaggagcta
     5941 gtagtcctac aaaagataaa ctacctgtca ccgctacact accgcttgcc aaagcaacag
     6001 ctaacaacaa aaggattttt tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt
     6061 cttgaccaaa tgatagcaat acaatcctct gtgcataaag tcctcctagc ggaattaaga
     6121 taaccgccca tggaagcaat gcccaaacat aaggccagct cgcaccccag attgatcccg
     6181 ctaaccatgc attcacaaat ccataatttt ccttagataa ttggatcgtg catagcaatg
     6241 ttaatgcaga aaggccggca ttaacggcaa taccggacaa taagattcga ttcataccca
     6301 aaaagccatt tcgatcatag gccgcaagat agacacagca agcagctaaa acaccaccta
     6361 gaaaggctac aactagcaat agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa
     6421 gcatcactaa aaatcctgct ccggcattaa tgcccaatat actagaatct gccaattcat
     6481 ttcttgttac tccttgcaag atataaccag aaacgccaag acaagctcct gctaataaac
     6541 tgatcagtaa tcgaggaact cgaaattctc taacaatcag cgccaaagaa gatgattctt
     6601 tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca atcattaaac
     6661 tacctataga caggatgaca agtacaaaaa gcaagaaata gaacgtccaa tatatttttt
     6721 tcaccgttgt tccctcctta cttgtatcag taaaaacgga acgccaatca atgcaatgac
     6781 taatccaaaa ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat ccgccaacat
     6841 aaccaatcca ccgccaacta aaaaagagac aggtagaaca taacgatagt ccgttccaac
     6901 cattccgcga acaactggcg gaaccaacag cccaataaat gaaacaggtc cgactaacga
     6961 aacggcaatg gctgctaaca ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg
     7021 atgacttcca agagctaatg catgtgtttc actcaaatga atcaaagata ttgcttttcc
     7081 taatgaaaaa gaaaccaaaa cagcaagtat atataaagga aacaatactg ctaattgttt
     7141 ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca agatcctgct gcagattaaa
     7201 gaattgggta agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa
     7261 aatcagctgt aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa
     7321 agttacagca aaagaacctg ccaatgacat tagaaacaca gaaaaatcag aagctttagg
     7381 ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc
     7441 tgaatctgct aaaggatttc ttgtcactcc ttgcatcaat gcaccgctca atgcaaaaca
     7501 actccctaca agaaatgctc ccgccacacg aggcaaacga atatttcgta tgatttggtg
     7561 actttgtttt gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt tttccacagt
     7621 cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa agaacaaaac agaccaaaag
     7681 caggagtcta tttttcttca tagtcctcat aattgatcct cctgctcttt tggcaacaaa
     7741 tcataggtta gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc
     7801 tcaaatattt ttttcataca ctcttttgtg atcacttctt caggtgtacc ttctgtgatc
     7861 aacttaccgt ttttcattgc taataaataa tctgaaaaac gtgaagcatg attaatgtca
     7921 tgaatcgaca tcaagatcgt tgtttgtttc atttgattga tttcagtcaa taactgtagg
     7981 atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt catccaagat caagatatct
     8041 gtttcctgag caagcgccat agcgatccag acacgctggg tttgtccacc agacagcgtt
     8101 gcgatatccc gcatacgaaa tccagctaga cctgtagcat ccaacgccca ctcaattgct
     8161 tcatgatctt gcgtgtccat gccactgaac agtttttgat aagggtatcg tccgtaagat
     8221 actacttctt ctactgtcat acctgtaggg tgttcactag tttgtgctaa taatgcaatt
     8281 ttttttgcaa gttcttttga actgtatgta gagatatcag agttattgac aatgatcttt
     8341 cctgatttca caggtagtat acgggtaaaa cttttcaata aagtagattt tccacttcca
     8401 tttggcccaa tcaaactagt gattttccct tcaggaatcg taacagacat gttttcaatg
     8461 atttttttgt tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt catttcaccc
     8521 atcctttcgc tgaaaatcat tctcaattat gacgctgttt caacatttcg tcaagcgcta
     8581 ctttcatatc tatagccaaa tgaagcggtt gacagcgttc gaaatatcag gaataataac
     8641 caatgagaaa gattctcaat tatgttagga ggaaagaaat gaaattaaaa acaaaagtat
     8701 tcacattatc tagcatattg ctgctggctg gagtactgag cgcttgctcc ccctcttcag
     8761 ataaagaaac agctacaact gccaattcga cgacttcatc caatagtact catacagtca
     8821 cagatacact tggacataaa gtagagattc caaacgcgcc caaacggatc atcggcagtt
     8881 acttagaaga ctatctgatt gcactagggg aaaaacctat cgcccagtgg actgttggca
     8941 gtggttccat tcaagattat ctgcaagatg atttaaagga tgtcccaacg atttcttatg
     9001 accttcctta tgagaaagtg ttaagctttg agcctgattt gctgttgatt tcttcttccg
     9061 ctactgtcga aggcgggaaa tacgaacaat atagcaaaat cgcgccaact tatgttgtaa
     9121 aaaacggaac agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa
     9181 aagaaaaaga ggcagaaaaa atcatttccg attatcaaaa atccatcaag aaaactcgag
     9241 aagaactagc agataaaatc gagaacaaga cagctgcagt gttgtgggtg accaacaact
     9301 cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac gatctggaat
     9361 ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc cacttctgac tggtctgctg
     9421 tttcctctga aaagctagct gaattagatg ctgactacat catccttgtg aacagtgaca
     9481 aagatgctgc catgttcaat gaaccaagct ggaagaatct aaaagcagtg acagacaatc
     9541 atgtactgca attcggacct gaatctagtt ggctatataa cggacctatt gccagtcagg
     9601 aaatggtcaa tgatatcaaa gaaaaactaa attgatttta actaaaaaaa gcatcttcac
     9661 gattagtcgt gagatgcttt ttttcttatt aacaaactag acgcatagat tttattcttc
     9721 ttttgaagca accctatttt ttactctttc ccatttttga aaacgatggg catatagatt
     9781 ttcttgatcc gttatcccct catactcctc tataagggcg aagtctgaaa aatcaatatt
     9841 cccaatatac gtatcgcctt caaacgtgct atcgatcaat gttcgccaga tccgaccagt
     9901 gtaaggaaga agttcttgaa aaatccgact accacccgaa acatagatat cttctgagat
     9961 cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt acttccgcat tttctttcgt
    10021 gcgaaaattt ggttgcgttg tcaacacgat gatatggcga taaggaagtg aaagatcccc
    10081 cattccttca taggtctttc ttcccatcac taaaattcga tccattgtat gttcgcgaaa
    10141 gaaacgcata tcatttggca atcgccatgg taacaaacca tttttgccga tcagaccatt
    10201 tttatcttgt gcccacatgg aaataaacat attatccgct ccttatgatt ttagatgttt
    10261 tgagataaaa aaagtctcaa ttctttgacc agcaaggaat tgagaacttt aaaaacatta
    10321 ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg caatattttt gtttttccaa
    10381 acgatcagga ttgttacgtt tgtttttgct tgttagatag ttacgttctt tacaagaagt
    10441 acactctaaa gtgatgttta cgcgcatgtt tttccctccc atttttacaa gaatcctcta
    10501 agatgactct cagacttgat tatcttacca tgattttttc tagattgcta gtgtttttca
    10561 cgattttgtt aagtaaaact acttgacgat aaatacaacg gttttacttc tcaaacaaaa
    10621 tctttttctt tttgtataaa tacccttaca cttgtttata tagattatgt tttttaataa
    10681 agggggatac taatatgcca tggaacatga aggactttcc tgcatcaatg aaaaacttag
    10741 ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat gaagggtacc
    10801 ctgataaccg tgcgatccca attgccattg atcaagcaaa aaaatggtat gaagatgcta
    10861 gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg
    10921 atacgaatcc tcgtgctgga aaactgcttg attcagatgt gatcgtagaa tatgaggaag
    10981 aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa
    11041 aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa agaatcaact ttgttcattt
    11101 ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg aacatatgta aaggggttgt
    11161 gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc
    11221 gaaccataag tcataatatc tataaaacgg gaagagctgt tttttgaaat tgtttcttat
    11281 cactcagtgt taaacggtag gagaaaacct cttttttatt gatatttgat tttcatcaaa
    11341 cagtcacata gtcgattgct tctgcttgcg gaatcgtttc atctacaaag tagactttat
    11401 accatgtcaa gaaactgaag attgtccaga tttttctttg ctcttcttta cgtccttcgt
    11461 gatggtcatc tagcaacttc atgatttttt cttgatcaaa aaattctttt gcaaaatctg
    11521 cttcgaataa tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg
    11581 gcacagggaa tcctaatttc acacgattcg accattcttc cggaagatgt ttgttcgcag
    11641 cttgtctgaa aatatctttt gtgttgtttt gattcaataa aaacttactt ggtatttgtt
    11701 gagccaattt catgacttcg atatccaata aaggtactcg gacttccaaa gaacttgcca
    11761 tagaaagttt atctgccttt aacaagatat cttttggcat ccattgatga agatcgacat
    11821 attgcatctt attgacttca tctttgattc cttctgtttt ctcgtaatga acagtcatga
    11881 tttcatccac agatggtgct gtttgatatt ttggtgttaa cacttctgat gcttctgatt
    11941 cttcgaacac tcgagcatga ccgataaaat attcttttgc aggagcaagt gattcatata
    12001 aatggatccg tccatggaag tttttcattt ttccgatttt acgtcctaaa ttatagcgtg
    12061 ttccttttgg taattttttc aatccttgag caatcacacg gatgaatttt gaattcgtat
    12121 ggaatccata cgtttggtat cccgcaaaca gttcatccgc cccttctcca gattggacta
    12181 cccgcacact ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa gaaggatttg
    12241 aatcaggttc atctaagtga tactggatca aagggaatgc tttgaacgac atgtctccgt
    12301 cgatcactgc tgctgtatta tctaagtcca atagttcggt tagttttctg gcttcaatcg
    12361 cttcattata ggttttatca tcaaacccga ttgagaaaga atgatctggt ttcaatacag
    12421 aggttacata actggaatca accccgctgg ataagaaaga accaacttct acatcactga
    12481 ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc
    12541 gggaatgcgt ttcttttgtt tcaaaatctg catcccaata ttgctgaatg tctatttttc
    12601 catctttata cgtataataa tggccttcag gcaaacgata gacatctttg aagaatgttt
    12661 cgccgtttaa tggagaatat tggaaagtca tataaggttt caacgcttct ttgttcaatt
    12721 ctttgttaaa atcaggatga ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc
    12781 cgttcatttc tgcataataa tatggtttga tcccaaagtg gtctcttgca ccgaataact
    12841 cattcttttc attatcccaa atagcaaaag caaacattcc gcggatcttt tgcaataatt
    12901 ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa
    12961 cgtggccagc tgcaatcaat tcttctctca aaggctggta attatagatt tcgccattaa
    13021 aaataatgat ctttgtccga tcttcattgt aaataggctg agtaccgccc tctaagtcaa
    13081 taatacttaa tcttctgaat cctaaagcaa catgtttatc gatatattct cctgaactat
    13141 taggtccacg gtgaacgatt cgatccatca ttgtattaat aattgttttt ttgttgtcct
    13201 tgtcgttaac aaaacctaca ataccgcaca tttaactttc acatcctaat ataaaattac
    13261 tgtttatttc attcatatgc atcttatcat agaataaata gcggacgaat attttctctc
    13321 ttacaatttc gtcacgtgaa aacaaaagat gaaagtttct gtttttacag aaaataaagt
    13381 cccaatatct aatttaccat gttttctttt ttgaggctac cttattctct tcgtgttaaa
    13441 aaaatattag tggatgctat ttccaaaaaa accagcaaaa aaagacacga cctaaagatc
    13501 gt
//
LOCUS       DAZKAY010000076        13347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000076 DAZKAY010000000
VERSION     DAZKAY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.077"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1365)
                     /locus_tag="ACG8WP_002088"
     CDS             complement(46..1365)
                     /locus_tag="ACG8WP_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028371.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="ACG8WP_002089"
     CDS             complement(1362..2015)
                     /locus_tag="ACG8WP_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9028372.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="ACG8WP_002090"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="ACG8WP_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2821..3540)
                     /locus_tag="ACG8WP_002091"
                     /pseudo
     CDS             complement(2821..3540)
                     /locus_tag="ACG8WP_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3565..3750)
                     /locus_tag="ACG8WP_002092"
     CDS             complement(3565..3750)
                     /locus_tag="ACG8WP_002092"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9028373.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3977..5250
                     /locus_tag="ACG8WP_002093"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="ACG8WP_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="ACG8WP_002094"
     CDS             complement(5357..6388)
                     /locus_tag="ACG8WP_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028374.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="ACG8WP_002095"
     CDS             complement(6395..7225)
                     /locus_tag="ACG8WP_002095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028375.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="ACG8WP_002096"
     CDS             complement(7222..8037)
                     /locus_tag="ACG8WP_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028376.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="ACG8WP_002097"
     CDS             complement(8034..8858)
                     /locus_tag="ACG8WP_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFP9028377.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="ACG8WP_002098"
     CDS             complement(8848..9948)
                     /locus_tag="ACG8WP_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9028378.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="ACG8WP_002099"
     CDS             10126..10911
                     /locus_tag="ACG8WP_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9028379.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="ACG8WP_002100"
     CDS             10944..11327
                     /locus_tag="ACG8WP_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9028380.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="ACG8WP_002101"
     CDS             11403..11534
                     /locus_tag="ACG8WP_002101"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9028381.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="ACG8WP_002102"
     CDS             11503..11739
                     /locus_tag="ACG8WP_002102"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9028382.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11751..11837
                     /locus_tag="ACG8WP_002103"
                     /pseudo
     CDS             11751..11837
                     /locus_tag="ACG8WP_002103"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11817..12788)
                     /locus_tag="ACG8WP_002104"
     CDS             complement(11817..12788)
                     /locus_tag="ACG8WP_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9028383.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4057 a         2912 c         2305 g         4073 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaaacaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttat
//
LOCUS       DAZKAY010000077        13250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000077 DAZKAY010000000
VERSION     DAZKAY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.078"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACG8WP_002105"
     CDS             complement(<1..451)
                     /locus_tag="ACG8WP_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028384.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKI"
     gene            complement(462..1130)
                     /locus_tag="ACG8WP_002106"
     CDS             complement(462..1130)
                     /locus_tag="ACG8WP_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9028385.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1261..1845)
                     /locus_tag="ACG8WP_002107"
     CDS             complement(1261..1845)
                     /locus_tag="ACG8WP_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9028386.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2180..2410)
                     /locus_tag="ACG8WP_002108"
     CDS             complement(2180..2410)
                     /locus_tag="ACG8WP_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9028387.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2425..3375)
                     /locus_tag="ACG8WP_002109"
     CDS             complement(2425..3375)
                     /locus_tag="ACG8WP_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP9028388.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3372..4214)
                     /locus_tag="ACG8WP_002110"
     CDS             complement(3372..4214)
                     /locus_tag="ACG8WP_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP9028389.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4347..5072)
                     /locus_tag="ACG8WP_002111"
     CDS             complement(4347..5072)
                     /locus_tag="ACG8WP_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9028390.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5533..5847)
                     /locus_tag="ACG8WP_002112"
     CDS             complement(5533..5847)
                     /locus_tag="ACG8WP_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9028391.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5863..7458)
                     /locus_tag="ACG8WP_002113"
     CDS             complement(5863..7458)
                     /locus_tag="ACG8WP_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9028392.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7481..8371)
                     /gene="iolE"
                     /locus_tag="ACG8WP_002114"
     CDS             complement(7481..8371)
                     /gene="iolE"
                     /locus_tag="ACG8WP_002114"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFP9028393.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8388..9413)
                     /gene="iolG"
                     /locus_tag="ACG8WP_002115"
     CDS             complement(8388..9413)
                     /gene="iolG"
                     /locus_tag="ACG8WP_002115"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFP9028394.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9437..10444)
                     /locus_tag="ACG8WP_002116"
     CDS             complement(9437..10444)
                     /locus_tag="ACG8WP_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9028395.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10557..11699)
                     /locus_tag="ACG8WP_002117"
     CDS             complement(10557..11699)
                     /locus_tag="ACG8WP_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFP9028396.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11793..13247)
                     /locus_tag="ACG8WP_002118"
     CDS             complement(11793..13247)
                     /locus_tag="ACG8WP_002118"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9028397.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3956 a         2872 c         2176 g         4246 t
ORIGIN      
        1 aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa
       61 gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa
      121 agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag
      181 gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg
      241 agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc
      301 cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa
      361 gactaccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat
      421 atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag
      481 aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag
      541 taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct
      601 tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga
      661 ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt
      721 tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc
      781 ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac
      841 aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcccaaaa
      901 aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact
      961 gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact
     1021 gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc
     1081 ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct
     1141 ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa
     1201 cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc ttttttttaa
     1261 ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag
     1321 catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga
     1381 gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat
     1441 agtctttaac ttatctagac aacagactgt cttttttgtc acaaatgatg taattttttc
     1501 aacaatatct tgatatttgg ttccagctga ttggaataaa accaaatcga atatacgttt
     1561 attctcaaaa atataattaa taaatggatc taattcattg aagttctctc tccaactttt
     1621 ctctatatct atattttcta agtttaagag gtatttggta aactcttcat tatacatatt
     1681 aataagcgca ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa
     1741 atgtttataa aaagcacccg tagttacgcc tgcggtcaag cacagttgcc ttatcttagt
     1801 ttgctgaaat cctacttttt caaaatggct tagtccactt tctattaatt tttggtgagt
     1861 tgtaatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat taactatatt
     1921 atcttacaca ggacttgaag tttcaatcgt taacgtctat tactgaactc attccttaga
     1981 gctaaactaa tgagaaagag caaaataact gatacaaaaa gcaatcctac aagaattaat
     2041 agactcatct aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat
     2101 atccagctat taggataagt cctatcataa tcactccaat agccattttc ttatcctccc
     2161 taaaaataaa gccttttact taaaaccttt ttttgtaatc gtcaataaat tttttccctt
     2221 tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta gtcagcatct
     2281 cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct
     2341 tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta gttgttttaa
     2401 tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt
     2461 ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt
     2521 aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc
     2581 gtccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa
     2641 gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga
     2701 aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt
     2761 tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt
     2821 ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc
     2881 taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg
     2941 gggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact
     3001 tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt tttactaatt ttgtaaacca
     3061 agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt
     3121 tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat
     3181 actgtgtaaa tcaattttat cttttgctat attttcagta atatctgcta aaatcgaatt
     3241 agtaactact acctttaatt tttcgttcga agccactgta tctttgtctt tactatttgt
     3301 tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt
     3361 ccattttctc atcataactt tctctccttc tttcttaaaa atttttgttt tggtgagatt
     3421 agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg
     3481 ttaaaactat aaccaatgaa caaacctagc aatgatgagc acgctccgac tgtcgccgaa
     3541 ataaatatca tagtttttaa actgtttgca tataaatatg ctgtagcagc tggtgtaatt
     3601 aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt
     3661 aatagaatca ttaatagata atgataaaaa ctgacattca tccccattgc ttttgccatt
     3721 aatggatcaa aagaagtgat taataattct ttaaaaaata gaacaactgt aaccaacact
     3781 aaaacagaaa cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca
     3841 aataagatat gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctaat
     3901 gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt
     3961 tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa
     4021 ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca
     4081 cccataagag acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct
     4141 ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata
     4201 aaattttgaa gcatattatt ccccttgtat attagagtta gggttttaac cttccggttt
     4261 tgattggata accctatgaa ccatcgttat tttaatttgg tattaactca tttcttttga
     4321 gataatatat ttattggatt gagaggctaa gccgttcccc cctctccaat aaatatagta
     4381 tctccatagg ctttttttaa attctcttca ttaaatacag attctacact tccgtgagca
     4441 attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt gcttaaatca
     4501 tgatgcacaa ttagcactgt ttttccctga tgtttcaatt cttttaacgt atccataatt
     4561 attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat gaaatccgca
     4621 ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga taattctcct
     4681 atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga aactctttgc
     4741 cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc catagataca
     4801 cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc tacatatgca
     4861 atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc ctgatgaggg
     4921 ataatattta acattgcttt aagcaatgta gattttccag caccatttgg accaataata
     4981 cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta
     5041 taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat gataatgatt
     5101 atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt tataatttag
     5161 atttttttct gttgttgaag attaggatgt caaaattatt tgaaactact cgtatagtaa
     5221 atagtaagta attttcaaaa cacgaagaaa aacaaaaaga aaaaaggaaa cgccgaagca
     5281 tttcctttca gtgatttaat gagaacaaca cgatttgact tagagtctca aaaagtcgaa
     5341 taggtacact catttaagtg tctccattcg actttttggg gatgagctgg ctattgaggt
     5401 ccgcttaccc tattgaataa atacaagatt gggtatttct aataagtccg cccttcatat
     5461 tggggtgatc ggggcgaaca tgccatcttt tcatatacca cctcctcgac actcaataat
     5521 caaagattct cgttaatctt cttcgatgct tgcaatgatg ttttcaatat tcatataacg
     5581 aaaatcttct tgtttgatat aaacgatctg agcggtacat ttttcctttt tgatgatttc
     5641 ttttactttt tcctcagaac gtgcgacctg cggcgttaaa agtaaaacgt catagtcttt
     5701 ataattcagt ttttcttcaa cgtttcggat cgttgcataa tctatctcat acgatagatt
     5761 tttggctctg atcgtttcaa gcatttttga tacgagcagc gacgcactga ttccggtctc
     5821 gcaaataatc aatagatcct tatgcataat accctcctgt ttttagcctg caaagccaat
     5881 tggtgaaaat gccacataag ttaaaataac aatgactacc aatgctatag cagtcacctt
     5941 ggtgtatttc caaggctcga tatcgacctt tgtatcgaat gagttcaaca caaattctcc
     6001 atcaggtttc aatttggaaa ccaccatcaa aatgataatg tccagaacga ataatgccgt
     6061 caaaacataa aggaaatgga tattttggaa aagcattttt gatagtgcat acaagacgat
     6121 atgcacggca atcgtcgttt ttgccccaaa agcagttgct ttttttgaat aaaaggcaaa
     6181 gagaatgatc acgagcagcg gcatgttgta tagtccattg aattcttgaa cgaactgata
     6241 caatccagcc ggtgcaaaag aaatgaatgg cgctacgata atggagataa tccccacaac
     6301 gaccgttact gttttaccag cacgagcgat ttgcttatca tttgctttct tattgataag
     6361 cggtttataa aaatccagtg aaaacaaggt cgatgtcgcg ttcaaagctc ccgcaaatga
     6421 tgataagatc gcgccaaaga taacagcggc gaaaatacca aagatcattt taggcaatac
     6481 ttcactgact aaaaaaggat acgcgttatc tggatttgac attaaactat cgccaaaaat
     6541 attgcgagcg acgatccctg gaaagacaag gaataacgca cctaagattt tgaagacgcc
     6601 gactaggaaa gcgccctttt gtgcctcagc taaattcttg ccggacaatg ctttttggac
     6661 gatcatttga ttggtacacc agaaatacag attgttaaac agcatccctg taaatagtgt
     6721 cggccatgga acaacagccg agtcgatcga tcccacagag ttaagcagcc atggcgtgtt
     6781 taccacaacg tgttcgatcc cgccaacaaa gctgccgtca ccaagcttca ttaagcctaa
     6841 aattgggacc agcagaccac aagccagcag accgactcca taaatggagt cactattggc
     6901 acttaacgac aagccgccga tcagcagata gaggatgccg acaataccaa ttgccatcgc
     6961 tacaattatg atcgcgacga tcggctggac gcccagcagc tcatcaatat ggaagatctt
     7021 attgaacacc agcgatccag aataaagaac cacgggtaag aaagaagtca tataggtcac
     7081 aataaataag atcgaaataa ttcgtttggt aaccgtatca taacgaatct cgatgaaatc
     7141 tgagattgta ttgatgccgt atttaaaata ttttggcaag aatatcacag ccaaagctac
     7201 gatcgcaatc gtagcagtta cttcccaggc cattacttcc atccctgcat gataactttg
     7261 accattttgt ccgactattt gttcggtgga caggttcgtc atgatgatcg tgccggcgat
     7321 tggcaaagca gttaagctcc gccctcccat gaagaaccct tctgatccag aagtatcgac
     7381 acttctggat ctagaatagg caaataacca tacacagatg acaatcacta ggaaactgat
     7441 tatagaaaaa agattcatcg tctagctcct tttcaataga ttaaagaccg gtgacctctc
     7501 gaatgtagct tctggctttg actgcatatt caaatggatt ggctttagcc gggtcctgtt
     7561 ctgcttcaac tacgatccag ccttcatagt cgctggcatg gattgctgtc cagattggag
     7621 tgaaatcaat catcccatca ccaggaaccg tgaagacgcc tttcttcacg ccgtttaaga
     7681 agctgtcttt ttccttttct acttcttcag ccgtttcttg acgaatatct ttgaaatgga
     7741 tgtgtttgat acgatcttga tgacggttgt aaaccgcgat tggattttca ccagaaaaga
     7801 ccaaatgtcc cgtatcgaac aataatttaa ctttgtctgg atcagtcatt tccattaaac
     7861 gatcgatttc ttccgtcgtt tgaacgccgg tacccatgtg atgatggtaa acgatctcca
     7921 tatccttttc gtgggccaaa tcccctaatt tttctaatcc ttccgccagt tttttccact
     7981 cttcatcagt gaaaactggt ttatcatcaa agactggcgc tgtcataatc ccttggatac
     8041 tatgaccctg ctcagctact acgatgactt tagcgcccat cgcatgtaag aaatcccgat
     8101 gtgctttgaa ggcttcggca gtttcttcaa aaggtttagt tgtcagaaac gcactgaacc
     8161 atgcactggc aacctttaag ccgcgaatat tcaaatattg ttccaacacc tctggatctt
     8221 ttggatattt gttgccaatc tctgttccgg tgaacccagc aagggccatt tcactgatac
     8281 attgttcaaa ggtattttca ctaccaagct cgggcatatc atcattggtc cacgcgattg
     8341 gtgcgatggc taattgaatt tgtttggtca tattctcaca ctcctgctta tttttttagt
     8401 tgtactaaac tcttcgtatc gaatgattct tgacaagcca acgcgatctt agttgattgc
     8461 aatccatctt ctgcattaac cgctggttga cgtttttctt gaatacatga gacaaattct
     8521 tgggcttcac gaatgaaggc ttctctgaat cgttctggga agttttgtga acatggacgg
     8581 atgatccctt gatcgttcat gatcgtcaca agatttttct caggcgcttg cgctacccgc
     8641 agcattcctt tggttcccat gatctctgtt tcgacatgat agccatgggc tgcgtttctg
     8701 ccggcaacta gtaaagctat tgttttatcg ctcaggctca tcattgcggc tccagtttca
     8761 agctcaccag cctgatccaa ttctggatac gccgcatttt tcccaatcgc ccacacttga
     8821 tcgacttctt ggccggtaaa ccagcgaacc aaatcaatat catggatcgc catatctaag
     8881 aacaagccgc cgctgtcgtt attcatggca aatttgacaa agctttccaa gccttcgctc
     8941 ggatcgatcc cataacaacg aatcagtgtg atttcgccga tttcgccatc ttctaccatt
     9001 ttcttcgcat actgatacga ctcatcatag cggcgcatga agcctaacat gaagacctgt
     9061 tcaggatgct cttcgatgac tttttgggtc tttttaatat cttcaacttc aaggcctaat
     9121 ggtttttcag taaagacatg cttgccattt tccatcgcca agcggacttg gtcacagtga
     9181 aagcctgatg gtgaaacaat aaagactgca tcgatcacgg aacttttgac catttcttca
     9241 tagctggaaa aagtatgctt gacgcctaat ttcttctgtg cgaatgctaa ttctgtttca
     9301 actaaactgc acgcagccgt aagctcacaa caactgacac ctgttgctaa attttgtgca
     9361 tgtacttttc ctaatcttcc taaaccgacg atgcctactc taatgttgtt catgaaatac
     9421 ctccgttttc tgagaattac tcattgtata gtgctggtcg ttcatccatt tgaatcatta
     9481 cttttgtttt tgtttcacga gactcatgac aagccatcat ggtgatcgct gctacatacc
     9541 catcccaagc ggtaggaccg acaaccgcat catttttgat cgaatcgatc cacagcttga
     9601 attcataatc ataggcatcc aaaaagcggg tctgccaatc tggtgaaaca ggtgtgtaat
     9661 ttttttcttc cgatttaatt cgtgtatagg ctgggtccgt cattcctact tctgccgttt
     9721 cgaaaaccac atcacattta atatcgtagc caaagcggca attcacaaag atttccaaat
     9781 caatcgttac accggatttt gtttgaaaga tcagcagctg cggatcatgc aaatcggcat
     9841 gggagttgct ggtttttttc ggcaagatca tttgaacaga ttcataatct tcatttagca
     9901 accagcgaag cacatcaatc tcatgaatcg cggtgttgct gatcgccatc ggtgttgtgt
     9961 agttttcatc tgcgctagga tttctatggg cacaatgcat cattaatgct tctccgtatt
    10021 gcttcgattc gattgcttct ttcagttcaa tgtagccgcg gtcatagcgg cgcatgaagc
    10081 caacctgaac caatttcttc ccataagcaa cttccgcatc aacgatccgg cggcagcctg
    10141 ctgcatccat cgctaatggc ttttcgcaga aaacatattt cccatgcttg attgcttcta
    10201 acacatactg ctcatgggtc gggtcccaag aagtcacaac aacgacatcc acatcttcag
    10261 aagcgatcaa tgcttctccg gtttcaaaga atttagcatc gacacgtttg gcaacctctt
    10321 tagcagcttt tacattgata tctgagactg ctgtcacata agccccttgt gttttctgat
    10381 tgatcctgtc gatatgctcg atgccgattg cacctgttcc gataacgcct acttttactc
    10441 tcaattactt atccccctgt tctgttgttt aagcacacat agtatgcgct tacaaaatta
    10501 ttcctgtgta gcttttattc ttaaaaggta gagccctctc tttgctccgc cttttactac
    10561 ccataagctt gtacgtataa ttccttaatt tgactatgac taggcacctt tttattattt
    10621 tgcggacttc cgctggtcaa cgcatccgcg gccattttgt ctaaggctgc cataaacgct
    10681 tgctgatcaa taccataatc tcgaatcgtt gggatcgaca gctgctcaca gagctcttgg
    10741 atttttttaa tcagataatc cgcagcctct tccagcgata aatcttcggc tgccggataa
    10801 ataaatctgg cgatcgtcgc taactcagtc tgaatagcat cctttgagaa ttcaaaaaag
    10861 accggcaata acatcgcatt tgatatccca tgaggaatgt gaaaaagtgc ccccaccggt
    10921 cgactcattc catgaatcaa tgtgactgac gcattcgaaa aggcaatccc cgcctccgtt
    10981 gaagctaagc tcatttgttc tctggcttca gtgtcgtcgc tattttcata tgcctgagaa
    11041 agcgtgttca aaattttctt catcgcttcc agtgaaaata gttgcgttac ttcattggct
    11101 ttgactgaaa cataagattc caaagcgtga catagcgcat cgactcccgt tgccgcggtt
    11161 acttttggtg gtacagtttt cgtaaaatca gagtcaacga ttgagatctg tggcatgaaa
    11221 acgagatcct tcatcatcat tttgactgct gtttttgtat tggtaataac tgccacatcg
    11281 gttacttccg aaccagttcc agcagttgtt gacactgcaa tcagcggaat gcccgcttgc
    11341 agctgatcaa aatttcttgc atgaatcgct tcgagactta attgattagc agccaaaacg
    11401 gctaccacct tggctgtatc cagacagctg ccgccaccaa aagcaatgat atgatcacat
    11461 tgatgcttct gaaaaagctt cagcgcatca taaacatttt cttgcgtcgg ttcggcttga
    11521 atgtctgtat actccgctaa ttgatcatct gtttttccca acagctcctt gatcttttga
    11581 agatgcccgg tctgttccat aaccgtatca ctaatgatta acggcttgtt gcctagttgt
    11641 tctgattctt ccataagaaa ttggattgtt ccgccgccaa ttcttaaatt ggcaggcatt
    11701 ttcagttgaa tattcacaga aaaaacctct ctctggctta tagcctataa cagaatcctc
    11761 gaagctcttt aacagagctt cgaagcaaat ctttaaatct cactttgcgc ataacgtgct
    11821 gtaacaactt tccgatgggt ataaaactca acactgtctt tgccattggc atgcaaatca
    11881 ccaaagaaag agtgcttcca gcctgaaaag gcaaaccaag ccatcggtgc aggcacccca
    11941 agattgaccc ccagcatccc agcatcaaca ttttctctga agtagcgaat cgctgatgcg
    12001 ttgttggtat acagacacga accattcgca aattcagatt cattcgtcag ctcaacggct
    12061 gccatcaaat cttttacccg cacgattgat gcgaccgggg caaaaatctc atcctgccag
    12121 atcttcatat ccatcgtgac attatcaaaa atcgttggtc caacaaaata gcctttttct
    12181 ggaatgtttt cgcgaccatc tagtaccagt ttcgctcctt catcgacccc tttttggata
    12241 tagtcaaagg tccgctgctg tgcatctttt cgaatcactg gtcctaaaaa gaccccttcg
    12301 tccaagccat tgccgatctt gatcgctttt gccgcagcct taaatttttc aacaaactga
    12361 tccgcgattt cctcttccaa aacaatgacg gaggctgcca tgcagcgttc accagcagaa
    12421 ccaaaagctc cgccaataag gtccttgact gcccgatcaa tattggcatc tttcagcaca
    12481 acaacatgat ttttcgctcc agtcaacgct tgtacccgtt ttagattttt tgttccttca
    12541 cggtaaacgt attcaccgac attttttgat cctacaaagc tgatgccttt taccagtgga
    12601 tcttgcagca atgtgttgac aacatcgtgg gcaccataga caacattaaa aacaccgcta
    12661 ggaaagcctg ctttttcaac cagctcaaca attttctcca tcaacaatgg tgttttttct
    12721 gaaggcttca gcaccaccgt atttcctgcc gccagagcca tcgggaagat ccagaagggc
    12781 accatcattg gaaaattaaa cggtgtgatc cctccgatta ctccgattgg gtaacgataa
    12841 ttcgtgatct caatatcagt cgccgctgtt gataaagaat ctcccatcat taaattggtg
    12901 attcccgctg cgtgctccac attttcgatt cctcgttgaa cttctcccag tgactcggac
    12961 aatgatttcc cattttccag cgtgatcaaa cgcgccaatt cacttttatg atctgttaaa
    13021 agctgatgca gcttgaacag ataacgtccg cgaacaggaa caggaacttt tttccaagtt
    13081 tgaaacgcag catcggcagc tttaactgct tcggctgtct ctgctttgct agacaacggt
    13141 acattcgcca gcgcttcttc tgtagccgga ttcattactt cttcaaactc tgttgagcga
    13201 ctagcgaccc aacgaccatc aatataattt cttaaaaatc ttgtcattta
//
LOCUS       DAZKAY010000078        12803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000078 DAZKAY010000000
VERSION     DAZKAY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.079"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2322)
                     /gene="esp"
                     /locus_tag="ACG8WP_002119"
     CDS             complement(<1..2322)
                     /gene="esp"
                     /locus_tag="ACG8WP_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP9028398.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
     gene            complement(2939..3292)
                     /locus_tag="ACG8WP_002120"
     CDS             complement(2939..3292)
                     /locus_tag="ACG8WP_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028399.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            complement(3389..4594)
                     /locus_tag="ACG8WP_002121"
     CDS             complement(3389..4594)
                     /locus_tag="ACG8WP_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9028400.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5288..5674
                     /locus_tag="ACG8WP_002122"
     CDS             5288..5674
                     /locus_tag="ACG8WP_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9028401.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5772..5963
                     /locus_tag="ACG8WP_002123"
     CDS             5772..5963
                     /locus_tag="ACG8WP_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028402.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6477..6809
                     /locus_tag="ACG8WP_002124"
     CDS             6477..6809
                     /locus_tag="ACG8WP_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9028403.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6719..6922
                     /locus_tag="ACG8WP_002125"
     CDS             6719..6922
                     /locus_tag="ACG8WP_002125"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9028404.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            6953..7099
                     /locus_tag="ACG8WP_002126"
     CDS             6953..7099
                     /locus_tag="ACG8WP_002126"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028405.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7106..7261
                     /locus_tag="ACG8WP_002127"
     CDS             7106..7261
                     /locus_tag="ACG8WP_002127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9028406.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7537..8877
                     /locus_tag="ACG8WP_002128"
     CDS             7537..8877
                     /locus_tag="ACG8WP_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9028407.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8913..10418)
                     /locus_tag="ACG8WP_002129"
     CDS             complement(8913..10418)
                     /locus_tag="ACG8WP_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028408.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIRLFVNECW"
     gene            complement(10415..11101)
                     /locus_tag="ACG8WP_002130"
     CDS             complement(10415..11101)
                     /locus_tag="ACG8WP_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9028409.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(11294..12715)
                     /gene="gndA"
                     /locus_tag="ACG8WP_002131"
     CDS             complement(11294..12715)
                     /gene="gndA"
                     /locus_tag="ACG8WP_002131"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9028410.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         4016 a         2473 c         2028 g         4286 t
ORIGIN      
        1 ggtgaaggtc actttgtggt agcctgcagg aatgttttct cctggatttt cataaatatc
       61 tttacttaca gttactgcta aatcggtcgt gcctacagaa ccatcttggt aaacaatttt
      121 aataggaatt gttccagtaa tcggctccga ttctcctaca cctttagctg gagtgaatgt
      181 aacaatacca gtatttgcat caacacttgc attaccgaag tccttattat agacatctaa
      241 aattgaaaag gcatctttag ctaaaggaac atctggttta tcaaatgtag gttttccact
      301 ttgaactgtc gtttctggag ttgttgttat aggtacgtat gttgcatcat tttccatagc
      361 ttgagaagct tttacagcaa actctacatc cacgtcgaaa gttcgatttc cgatcttaac
      421 agttacaata cctttcgtac tatcagtaac gttagaagta tcaggctctg ttttccatga
      481 atactctgca tcttcaggta agtttttatt attccgaatt gattctttag catctggttt
      541 aaatccaact accacggttt gtttatcaac cgcttttggt gattccttaa taacggttga
      601 accttcttct ggtttatcaa aacctggaga aacgatttgg atttttaatt catctttcgc
      661 gattaattta cttgaatcta cacccgtaaa ttcaaattct acagtatctg aaattggagc
      721 cccatctttt tcatttggag cgatagtttt ttctgctaat acagtacctt ctttatcaac
      781 aagttgggct ttgtgacctg ttcccgctaa ctcgtggatg aatactttcc ccttaactgt
      841 tgtgtcaaca tcatatactt cttctaattc aggtttcccg atatatggat tcgtgtctcc
      901 gctctcttct ttatcgtcag ggtttccatc accatcaata tcttgcaaat aataataacc
      961 tgttccataa gtgttctgga ttaatttttt attcgtatcc gacaaccaag aatcaaagat
     1021 gaaatcttca ccaaatggtt tattttcttc aacttgtgct gttgctttca tatcttctaa
     1081 gatttcatct aatggcttag tcaattgtaa aacatatcga ataacgactg gacgtggact
     1141 tgcattagca aaatcaataa gtttggtaag atcattatca gtaattgaac ctacaccata
     1201 attttcatga tctttgcgcc gttcaattgc taaatcagcc acttttttcc ctgattcaaa
     1261 agaaacatca tatgttactt tgtcagcctg aaccatgtgc atctgtatac ccgctacata
     1321 tggaactaat cgttcatcaa ttttgtaatt agcataccaa ggcgtgccct ttgcggaagc
     1381 tgaatatgag aaattataat ttttcgttaa cttatgatct aagattatag ctccattagc
     1441 acctgtggat cctgaaccat cttttgtttg agtattaggt tcctcatatt ttgcacttac
     1501 tgatccatgt ttaaactggt caggattatt ttcagataca tctaagctat tatagaaatc
     1561 atctgaatct gttaaaaagt agcccgtact atcaatacct ggtacaattt tatcatcgtc
     1621 ttgatgactt accaagtaga ttcgatacat taaacgatcg gtcttgagat tgtctatgcc
     1681 tatctcttta aaaatttcat ctaagctctt ttcaaacgta attaccccat taggagcagt
     1741 ttgtgtatca gttgtttcag ccccagcaaa taatccatca cttgcacgaa tataattgac
     1801 cttccaaata tttgtaggtc tacccagcga atcattaatt cttacaaagg tccgtctaga
     1861 agatccaatt cctttctttg gatcgacctg aattttttct acatatttag ctaatcgttc
     1921 atctaattga agacggattt cccagttttt agttgatgct gcaatattat gttttccgta
     1981 aacttcaaat ccaattgtat tgccttttag tgtgtctcca gttagttctt gaggactaaa
     2041 ttgtaaatcc gaaaagttat aatttttcgt ataaccagga ttctcctgaa aagtattccc
     2101 cgaagctact ttgtcaccta cttgtacgga gcttatcgct gtttcaagtg gggtgcttgg
     2161 agaaatttca tcttgtgctt gaactagttc agttgttccc aagacaagac caacaccaac
     2221 caaaacagat gcaaccccaa cacttaattt tttgatactg tacttcaata ctcgtttctt
     2281 tgttttttct agaaatactc tcttattatt cttgctaacc attattcaaa tcaactccct
     2341 taatagttat tttcttagta agaataaaag ataaatctga tacgtattat tcacagaata
     2401 ggaatcttac taaacttatg ttaactagtt tacttaaaga atttcatgca cattacaaca
     2461 aaaaaagtac atttcttcat gtttttttcg gatatttgtg ctttttaacc attcagcagc
     2521 tgaaaacata taaaaagata tgtatgtatt ctttctgaaa ttaagttcat ttctatgcga
     2581 tgaagcatgt tttcaaaata ttactctttc ttagattaaa cttcagagta ttcaaatagc
     2641 ggaataagga gccttatccc gcattactga atgagactgt gacataagta aaaaggtgac
     2701 ttcaaattcc ttcttcggaa tttgaaatca ccttctttat aattataaag tatcggtttc
     2761 acagcattcc tgaatgattc ctagaatctt aaacgaatga ttggagttat caacattttt
     2821 tcactcgttt ttagcgaaac aaaaaatgac atttgtcaca gcctcattaa attattgatt
     2881 ctttttcttt ataattgaat tgtccatatt taaagtgtct agtactctac atgaataatt
     2941 aattgctttc actcaaatac ttataatcta ttaacttgta gttgtttaac ttttctgaaa
     3001 aagttagacc cacgtaataa tcttcccagc tttctctttc aattaactga tccaattttt
     3061 gataatccat aaattcatca tgtattaaaa agaataaata acttgatatt ttagcatcta
     3121 gcttaatcag attaaataat gtttgcttaa aattgctacc attaatatga ctgatagatt
     3181 ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca tattcattta
     3241 acacactatt attaccatta cttagacgct cagattctac gaaacaactc aaatcagaaa
     3301 tttcccaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac tacgtaaatg
     3361 atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaatta tccactaaac
     3421 ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc gaaaagtgtg
     3481 aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt gatttcaaca
     3541 ttattttagc cgtagaaatt tttcgcttag agatataatt ataaatcgta atatgcattt
     3601 cattcttaaa gctttctctt aatttagttt cacttacaga aaaataatta gctatttcct
     3661 ttacagtaat tcttttatag atatttaagc cgatgaattg aacgacagaa gaaattaagg
     3721 gagaaagatg ctctatctta gtttttccga tttccttagt ataataaaca atcccagaat
     3781 ccctcacttt taagacctca ggtaacgata cagctaattc gttttcttgt atcaaagcat
     3841 ctctactttg atacgcttct attatatcca ttcctaatgt tatagacgtt tgtgctaact
     3901 tttcaaaaac aatgatagaa taattttttt cagaccgaat cgtatctcca ctaacacttg
     3961 gaattattcc attactcaga ttaaaaacca tgttttctaa atttcgaata tctccatttt
     4021 taacatattc cagtatttta ttttcataaa gaaacagata tatttctgga tcgtaatttt
     4081 gtgataacag attatctatt ttgatttgct cgatagtttt acttagattt cctgtatatt
     4141 caattaacgg ttcatggaat agatcttcta tttttccagt aaaaaaataa tgaactaaaa
     4201 tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa attttgcaat gaattataga
     4261 gtcgttctct atcagccatt gcaacattca aattgttcat ttttagttta aaaaaatctt
     4321 tatcaatatt atttgctcta aatggaccaa ataagtaata tctggtttta aacatgtata
     4381 gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa atctttagtt tttttaatat
     4441 tatcagttag taacttttta taatcataaa aaaaataagt agttctatct gagcgatact
     4501 cttttataat actgaattcg gaattaaaaa ccacgatagg taactttaaa caaacatgaa
     4561 gagactttaa taagacttct ttatctgaaa acacttttgt ttctctcttt cctttctaca
     4621 tcattaaata gtattttata cgttatctaa aatactattc ctctgtcaaa ataagctaac
     4681 ttacttcatt ataaaatatc cacttatcgc tttcatgtat attccaccgt aaaaagtaca
     4741 ttttatcacc gagaactcat catttttcat catgatgaat actatttaca tatatctagt
     4801 tcaaagacaa taattattat gatccgttat tatctaagca ctatacagat aataggaatt
     4861 tttcgtttct aaatactcca atttgggaat ccaataaaaa ttttcgttca aatacgtgtc
     4921 tattcgaact cttttctagt tgttaattat gaagggggaa gaaataaaaa atacaatcta
     4981 tacacgaata agaatattat ccaaaaggag gttttagaaa agatttatta tacgtaaaaa
     5041 gctcaagaca ttttcttagc cataaggaca tgtttgatat ttaagaagat atatgaattt
     5101 tcttttctaa gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa
     5161 acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat
     5221 tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt
     5281 gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa
     5341 ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga
     5401 acaacttgat caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc
     5461 gattaatgtt ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc
     5521 tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa tttatcttta ataaaactga
     5581 taaagaaatt ggaaaggttc tgggtattac aagacagggt gcgacaaatt taaaacatcg
     5641 actttacaaa aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat
     5701 gcttctttgc tcgtcctcct ccttccccaa aaaataaata gattataagt tcatgggaag
     5761 aaggtaaatt tatgaatact ttaaaagaag atttcatttt agcaaagaat ggaaatgaag
     5821 aagcagttgt ggccattctc aaaagattta gcgctttggt ccataagcaa tcctggagaa
     5881 ctggaaaata tgatcaagat tgttatcaag aatgtatgct tgcaatatat ctagctatct
     5941 cgaaattcga aattaaagaa taactcaact aagggagtct gtgacataag taaaaagata
     6001 gtttcaaatt tcgaacaata aatttgaagc catcttcttt ataattataa agtatcgatt
     6061 ttacagcgtt ctataatgat tcatagaatc taaaacgaat gattttaatt attaacgttt
     6121 tttaattcgt ttttagctta aaaataactt ttgtcacagt ctcactccta aaattacttt
     6181 atttacgtac gaagtgggca ggaggtcgaa tccagaactg ggccatggta cttcaacgag
     6241 gacttctcct ctagaattga aaagcatgcg atttaccttc cgtaacgacg gtagatttat
     6301 tcaactatgt gtccaatcaa aatttgtctg atttcaaaaa tagtcaagag aattttggtt
     6361 gttaaaaact cttatctatc aacttccaca gtttagttga caaaccctga tactaagttg
     6421 ggctatgata cacttttctt gactatcagt tacgttaccg gaaggaatga actctattga
     6481 aaaaaacata tgatcgtaaa tttaaactaa agatttcaca ggatatcctt gagaaaaaag
     6541 taactaccaa gaaaattgct gaagaataca atatctctcg tccaacaatt tcccgatggg
     6601 tttcagaata tcgtcgatac gggaaaaatg cttttgctgg acaaggaaaa agattacctg
     6661 ataaagcaga tttctacatt cttgaacagg aaaataaacg actaaaagag gaagacgagt
     6721 gttctacgaa cacaaagatc gatacggatc tccagggatc actcaaaaac tttacgatga
     6781 aggaatcgaa atcaataaac gtgttgtagc tgttcttatg cgtgaaatga atttatgcac
     6841 aagaggattt catcgacgca aatcttctca tggtagagaa aaaacaatca aagaaatggt
     6901 aaaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt gggtgacgga
     6961 tattacttac attccatgta atgacggtcg cctctatttg tccacctata ttgacttagc
     7021 aactcgtatt ctacgttact atatggtgga ttcacacatg aaaaaagaga ttgtcgtccg
     7081 tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata gaggtagcca
     7141 gcatcggtct tttatttatc aagagctgtt gaccgaccac cacatcacac attcaatgag
     7201 ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat caatcaaatg
     7261 agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt taatccaaga
     7321 ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga tgactccttg
     7381 acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga cgccttctgt
     7441 tgccaatact ttttcttttt tctaataaac gcaaacagac accttgaaat cctttagaat
     7501 aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga gtttaccaaa
     7561 gagttactag atattctaga cctaaatctt acctttcatg aagatgcctt tcgcaaagag
     7621 cgcattaatg acgaaacatg ttttgttttt gatggcacat tgacctatca accagaggaa
     7681 tgcttccatt gccactatca aaataagcaa acgatcatta aatggggctg gaaaaaagta
     7741 tccatcttat tgaatgatgt cagtaactac aagaccattg tgcggattaa caaacagcgc
     7801 ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag cgatcgtcgc
     7861 tgctccattg cccgcagagt caaacaagcc attcttgagc tattaagtga gcctatttct
     7921 atgtctttga ttgcacgtat gaagcacatt tctccgacca ctgtcattcg aatacttcgc
     7981 agccttcggc ctaaaaccgt ttctttgaac cagccgttac cagaagtggt gtgtttcgat
     8041 gaattcaaat cggtgaaaaa cgtctcggga gccatgagct ttgtcatgat ggacggcaag
     8101 acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg cgactatttc
     8161 ttgcgctacc ctagaaaagt ccgtgaacaa gtacgtctag tggtttcaga tttctataca
     8221 ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc cgatcgcttt
     8281 catattagcc aacacatcgg tcgggccttt accaaccacc gaattcaagt aatgaagacc
     8341 tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggagact acttcaaaag
     8401 aatgcgtggg aattgaatgg tcagcatcgc tactggcgtc cgtcatttcg agatcatctg
     8461 acagaagcgg agatagtcga tcggctatta tattatgatg actccttaaa gcgaggctac
     8521 gaagtctacc aggtttttct ttccgctatc agaagacaag atgttcctga attcgtcgcg
     8581 ctcctaaaag aagactacaa ggagttgcca gagcactatc aaccagtgtt cacgaccttc
     8641 aagaaatatc gaacagaaat caaacgagcg ttgcgagttc cctattccaa cggccctata
     8701 gaatgtttga acaaccatat caaagtacta aaacgaattg cctatggctt tagaaacttt
     8761 caaaactacc gagaacgaat ctttttatat cgaggaaaat atttcaagaa aactaagaac
     8821 actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc tgtctagccg
     8881 tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac gaagagccga
     8941 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
     9001 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
     9061 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
     9121 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
     9181 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
     9241 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
     9301 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
     9361 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
     9421 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
     9481 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
     9541 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
     9601 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
     9661 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
     9721 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     9781 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     9841 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     9901 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     9961 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
    10021 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
    10081 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
    10141 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
    10201 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
    10261 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
    10321 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
    10381 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
    10441 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
    10501 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
    10561 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
    10621 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
    10681 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
    10741 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
    10801 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
    10861 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
    10921 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
    10981 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
    11041 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
    11101 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
    11161 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
    11221 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
    11281 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
    11341 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
    11401 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
    11461 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
    11521 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
    11581 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
    11641 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
    11701 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
    11761 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
    11821 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
    11881 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
    11941 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
    12001 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
    12061 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
    12121 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
    12181 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
    12241 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
    12301 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
    12361 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
    12421 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
    12481 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
    12541 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
    12601 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
    12661 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
    12721 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
    12781 tgcaaactaa agaacaacat att
//
LOCUS       DAZKAY010000079        12738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000079 DAZKAY010000000
VERSION     DAZKAY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.080"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8WP_002132"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8WP_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9028411.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8WP_002133"
     CDS             complement(323..892)
                     /locus_tag="ACG8WP_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9028412.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8WP_002134"
     CDS             1078..1980
                     /locus_tag="ACG8WP_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9028413.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8WP_002135"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8WP_002135"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9028414.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8WP_002136"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8WP_002136"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9028415.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8WP_002137"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8WP_002137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9028416.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG8WP_002138"
     CDS             8548..8733
                     /locus_tag="ACG8WP_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9028417.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG8WP_002139"
     CDS             complement(8795..9991)
                     /locus_tag="ACG8WP_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9028418.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG8WP_002140"
     CDS             complement(10161..11597)
                     /locus_tag="ACG8WP_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9028419.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG8WP_002141"
     CDS             11890..12699
                     /locus_tag="ACG8WP_002141"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9028420.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2672 c         2250 g         4013 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtctaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DAZKAY010000080        12545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000080 DAZKAY010000000
VERSION     DAZKAY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.081"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1932
                     /locus_tag="ACG8WP_002142"
     CDS             49..1932
                     /locus_tag="ACG8WP_002142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9028421.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2007..2291)
                     /locus_tag="ACG8WP_002143"
     CDS             complement(2007..2291)
                     /locus_tag="ACG8WP_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9028422.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2281..3015)
                     /locus_tag="ACG8WP_002144"
     CDS             complement(2281..3015)
                     /locus_tag="ACG8WP_002144"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9028423.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3144..3767
                     /locus_tag="ACG8WP_002145"
     CDS             3144..3767
                     /locus_tag="ACG8WP_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9028424.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3959..5080
                     /locus_tag="ACG8WP_002146"
     CDS             3959..5080
                     /locus_tag="ACG8WP_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9028425.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5087..8215
                     /locus_tag="ACG8WP_002147"
     CDS             5087..8215
                     /locus_tag="ACG8WP_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028426.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8234..11428
                     /locus_tag="ACG8WP_002148"
     CDS             8234..11428
                     /locus_tag="ACG8WP_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9028427.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11574..12281)
                     /locus_tag="ACG8WP_002149"
     CDS             complement(11574..12281)
                     /locus_tag="ACG8WP_002149"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9028428.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4272 a         2211 c         2646 g         3416 t
ORIGIN      
        1 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
       61 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
      121 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
      181 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
      241 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
      301 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
      361 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
      421 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
      481 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
      541 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
      601 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
      661 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
      721 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
      781 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
      841 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
      901 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
      961 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
     1021 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
     1081 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
     1141 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
     1201 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
     1261 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
     1321 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
     1381 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
     1441 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
     1501 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
     1561 ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca
     1621 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
     1681 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
     1741 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
     1801 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
     1861 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
     1921 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
     1981 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
     2041 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
     2101 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
     2161 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
     2221 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
     2281 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
     2341 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
     2401 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
     2461 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
     2521 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
     2581 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
     2641 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
     2701 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
     2761 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
     2821 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
     2881 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
     2941 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
     3001 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
     3061 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
     3121 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
     3181 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
     3241 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
     3301 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
     3361 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
     3421 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
     3481 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
     3541 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
     3601 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
     3661 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
     3721 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
     3781 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
     3841 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
     3901 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
     3961 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
     4021 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
     4081 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
     4141 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
     4201 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
     4261 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
     4321 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
     4381 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
     4441 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
     4501 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
     4561 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
     4621 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
     4681 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
     4741 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
     4801 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
     4861 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
     4921 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
     4981 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
     5041 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
     5101 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
     5161 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
     5221 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
     5281 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
     5341 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
     5401 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
     5461 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
     5521 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
     5581 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
     5641 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
     5701 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
     5761 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
     5821 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
     5881 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
     5941 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
     6001 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
     6061 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
     6121 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
     6181 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
     6241 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
     6301 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
     6361 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
     6421 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
     6481 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
     6541 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
     6601 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
     6661 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
     6721 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
     6781 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
     6841 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
     6901 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
     6961 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
     7021 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
     7081 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
     7141 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
     7201 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
     7261 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
     7321 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
     7381 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
     7441 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
     7501 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
     7561 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
     7621 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
     7681 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
     7741 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
     7801 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
     7861 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
     7921 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
     7981 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
     8041 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
     8101 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
     8161 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
     8221 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
     8281 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
     8341 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
     8401 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
     8461 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
     8521 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
     8581 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
     8641 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
     8701 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
     8761 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
     8821 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
     8881 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
     8941 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
     9001 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
     9061 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
     9121 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
     9181 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
     9241 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
     9301 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
     9361 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
     9421 cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
     9481 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
     9541 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
     9601 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
     9661 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
     9721 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
     9781 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
     9841 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
     9901 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
     9961 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    10021 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    10081 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    10141 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    10201 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    10261 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    10321 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    10381 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    10441 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    10501 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    10561 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    10621 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    10681 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    10741 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    10801 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    10861 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    10921 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    10981 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    11041 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    11101 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    11161 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    11221 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    11281 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    11341 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    11401 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    11461 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    11521 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    11581 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    11641 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    11701 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    11761 atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    11821 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    11881 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    11941 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    12001 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    12061 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    12121 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    12181 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    12241 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    12301 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    12361 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    12421 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    12481 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga
    12541 tttat
//
LOCUS       DAZKAY010000081        12432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000081 DAZKAY010000000
VERSION     DAZKAY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.082"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACG8WP_002150"
     CDS             complement(<1..190)
                     /locus_tag="ACG8WP_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028429.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACG8WP_002151"
     CDS             complement(200..1015)
                     /locus_tag="ACG8WP_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9028430.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACG8WP_002152"
     CDS             complement(1277..1720)
                     /locus_tag="ACG8WP_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP9028431.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACG8WP_002153"
     CDS             complement(1854..2192)
                     /locus_tag="ACG8WP_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9028432.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACG8WP_002154"
     CDS             complement(2180..2557)
                     /locus_tag="ACG8WP_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9028433.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACG8WP_002155"
     CDS             2781..3974
                     /locus_tag="ACG8WP_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9028434.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACG8WP_002156"
     CDS             complement(4140..4562)
                     /locus_tag="ACG8WP_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028435.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="ACG8WP_002157"
     CDS             4957..5151
                     /locus_tag="ACG8WP_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028436.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="ACG8WP_002158"
     CDS             5148..5594
                     /locus_tag="ACG8WP_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028437.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="ACG8WP_002159"
     CDS             complement(5804..6976)
                     /locus_tag="ACG8WP_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9028438.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="ACG8WP_002160"
     CDS             complement(7182..7997)
                     /locus_tag="ACG8WP_002160"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9028439.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="ACG8WP_002161"
     CDS             complement(8147..8593)
                     /locus_tag="ACG8WP_002161"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9028440.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9105)
                     /locus_tag="ACG8WP_002162"
     CDS             complement(8590..9105)
                     /locus_tag="ACG8WP_002162"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9028441.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKQ
                     QQKGNNRREIK"
     gene            complement(9178..10092)
                     /locus_tag="ACG8WP_002163"
                     /pseudo
     CDS             complement(9178..10092)
                     /locus_tag="ACG8WP_002163"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10089..12416)
                     /locus_tag="ACG8WP_002164"
     CDS             complement(10089..12416)
                     /locus_tag="ACG8WP_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP9028442.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2273 g         4216 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttgtt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DAZKAY010000082        12291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000082 DAZKAY010000000
VERSION     DAZKAY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.083"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG8WP_002165"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG8WP_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9028443.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG8WP_002166"
     CDS             complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG8WP_002166"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9028444.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG8WP_002167"
     CDS             complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG8WP_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9028445.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG8WP_002168"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG8WP_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9028446.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="ACG8WP_002169"
     CDS             3621..4100
                     /locus_tag="ACG8WP_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9028447.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="ACG8WP_002170"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="ACG8WP_002170"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9028448.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="ACG8WP_002171"
     CDS             5401..5529
                     /locus_tag="ACG8WP_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9028449.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="ACG8WP_002172"
     CDS             5533..6078
                     /locus_tag="ACG8WP_002172"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9028450.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG8WP_002173"
     CDS             complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG8WP_002173"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9028451.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG8WP_002174"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG8WP_002174"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9028452.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG8WP_002175"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG8WP_002175"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9028453.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG8WP_002176"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG8WP_002176"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9028454.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3380 a         2534 c         2208 g         4169 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa atatttattc
    12241 attgaaaaaa tttttttgaa taaattaaaa aaataaatta tattatcgtt g
//
LOCUS       DAZKAY010000083        12160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000083 DAZKAY010000000
VERSION     DAZKAY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.084"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..730
                     /locus_tag="ACG8WP_002177"
     CDS             158..730
                     /locus_tag="ACG8WP_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9028455.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="ACG8WP_002178"
     CDS             875..1579
                     /locus_tag="ACG8WP_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9028456.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="ACG8WP_002179"
     CDS             1807..2145
                     /locus_tag="ACG8WP_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028457.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8WP_002180"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8WP_002180"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9028458.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="ACG8WP_002181"
     CDS             3131..3364
                     /locus_tag="ACG8WP_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9028459.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="ACG8WP_002182"
     CDS             3562..5466
                     /locus_tag="ACG8WP_002182"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9028460.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="ACG8WP_002183"
     CDS             5776..6138
                     /locus_tag="ACG8WP_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFP9028461.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="ACG8WP_002184"
     CDS             6155..6766
                     /locus_tag="ACG8WP_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFP9028462.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="ACG8WP_002185"
     CDS             complement(6868..7776)
                     /locus_tag="ACG8WP_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9028463.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="ACG8WP_002186"
     CDS             complement(7818..8132)
                     /locus_tag="ACG8WP_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9028464.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="ACG8WP_002187"
     CDS             8431..10362
                     /locus_tag="ACG8WP_002187"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9028465.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="ACG8WP_002188"
     CDS             10655..11206
                     /locus_tag="ACG8WP_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028466.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="ACG8WP_002189"
     CDS             11519..12061
                     /locus_tag="ACG8WP_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFP9028467.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3951 a         2034 c         2555 g         3620 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt
//
LOCUS       DAZKAY010000084        11787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000084 DAZKAY010000000
VERSION     DAZKAY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.085"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8WP_002190"
     CDS             complement(<1..182)
                     /locus_tag="ACG8WP_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028468.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISG"
     gene            complement(198..812)
                     /locus_tag="ACG8WP_002191"
     CDS             complement(198..812)
                     /locus_tag="ACG8WP_002191"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9028469.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(959..1642)
                     /locus_tag="ACG8WP_002192"
     CDS             complement(959..1642)
                     /locus_tag="ACG8WP_002192"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9028470.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1751..2209)
                     /locus_tag="ACG8WP_002193"
     CDS             complement(1751..2209)
                     /locus_tag="ACG8WP_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9028471.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2293..4056)
                     /locus_tag="ACG8WP_002194"
     CDS             complement(2293..4056)
                     /locus_tag="ACG8WP_002194"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9028472.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4277..5737)
                     /locus_tag="ACG8WP_002195"
     CDS             complement(4277..5737)
                     /locus_tag="ACG8WP_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028473.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5945..6871)
                     /gene="glsA"
                     /locus_tag="ACG8WP_002196"
     CDS             complement(5945..6871)
                     /gene="glsA"
                     /locus_tag="ACG8WP_002196"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9028474.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6894..8321)
                     /locus_tag="ACG8WP_002197"
     CDS             complement(6894..8321)
                     /locus_tag="ACG8WP_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9028475.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8352..9566)
                     /locus_tag="ACG8WP_002198"
     CDS             complement(8352..9566)
                     /locus_tag="ACG8WP_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9028476.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9652..9879)
                     /locus_tag="ACG8WP_002199"
     CDS             complement(9652..9879)
                     /locus_tag="ACG8WP_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028477.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9907..10149)
                     /locus_tag="ACG8WP_002200"
     CDS             complement(9907..10149)
                     /locus_tag="ACG8WP_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9028478.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10181..10738)
                     /locus_tag="ACG8WP_002201"
     CDS             complement(10181..10738)
                     /locus_tag="ACG8WP_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9028479.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10751..10939)
                     /locus_tag="ACG8WP_002202"
     CDS             complement(10751..10939)
                     /locus_tag="ACG8WP_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9028480.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10952..11518)
                     /gene="amaP"
                     /locus_tag="ACG8WP_002203"
     CDS             complement(10952..11518)
                     /gene="amaP"
                     /locus_tag="ACG8WP_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9028481.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3453 a         2446 c         1932 g         3956 t
ORIGIN      
        1 ccgctaatcg tttgatctgg catttctatt tccttaccat ctaaagtaac agtagtaacg
       61 acaaaatggt cttgcaatgg cggaacttct aagtactgcc cttgcagaaa atcattcact
      121 gtttcatatt cttttttcat ttcttttgaa tctgtcgatg gtttatagct tatttttaac
      181 ataactagtg ctccttttta ttcgggacgt gcaatcgtga aaatttctcc tattgctgca
      241 taatcatttc ctgccaaacg gcttacagca ttcaattttc ttggatcgat ccttcctgtc
      301 ttatcatcat agactgtttc atttaaatga tatttctgta cttctagcaa aaataaatca
      361 gctgttactt tatttttatt ccgtatctcg acatgttcat ataaggatgt ttcaaaacga
      421 acttttgctt ctgtcaatgc tggtactgaa atgattactg aatcagttaa agtcaagtca
      481 gtcaggtaca gttcgctttc ctcattaggc agattggctg ctgttttatt tacttcttct
      541 acgttgtcct catcaaggat atggataaca gcttctcttg tctcaagaat attctttgct
      601 gtgtctttta ctgcatcttg ttttctctgg atcgccaagg ataagatggg tcgattcgaa
      661 ctgacgatat tgaagtaact gaagggtgcg atattcacta tatcttttgc tgatttagtc
      721 gttactactg ctactggtct agggatgact gaaccaatca gcaacttgta attttcacgt
      781 tctgtcaatt ctgaaggact ccgttcaaac atggtttcgc cttctttcta taaaattcgt
      841 taaaaagcag atcttgaaga caaaatgaaa agtaatgctt ttttcatctt cttagaatct
      901 gctttaaact atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg cgtgtttttt
      961 agttatttgc tttacttgcg gcttcgacat atcttttagt gatcaattgc ggacgggtga
     1021 taatagatcc aactacgact gaaaaagcac ctaattcata tgctttctct acttgttcag
     1081 gagagtcagt atgtccttcc ataattactg ggatatcgac ttgttctatg atttgattca
     1141 ataaatcaaa atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct gctaaagtcg
     1201 tagacaaaag atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa gtcgaaatat
     1261 ctgccatagc taaccgaccg tgttcgtgaa tcatttggac aagttcagca gttgtttttt
     1321 ctgtcggacg tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc gtctccaaaa
     1381 gttcttccac ttctttttct gtcgcagtaa taaacacttc tgacccatcg taatctcttt
     1441 tgataatccc tattaccggt aaatcaacga ttcgcttgat ttcattgata tcttctgtcg
     1501 tatttgctcg aataccaact gctccgcctt cttttgcagc caaagccatt ctccccataa
     1561 taaaagaact gtgtagcggc tcgttttcca aagcttgaca agaaacgacg actccgcctt
     1621 tgatttgttc cagtgtatcc acaatatctc ctccagtaat gttatataaa aatacagctt
     1681 catataaaaa tgtaaccctt ttctattcag gagacaataa tgagctattt ctttcagcag
     1741 acctttctta ctaaaagaaa attacttcaa attcttcgta tacgaccagc ttctcttctg
     1801 taaattctat gttcaacgct tctttataag catgtttaaa aaactcagca tggactttcc
     1861 gccaataagc taatgtccga tccccttctc cttctttcca agcatgctct ttcgtgactt
     1921 cactaaaagg caagatggtc acatttatat ttttgatgat acaaactggt tcattttttc
     1981 cattcaggac cacgtctaat tgaccaactt ttggtaaagg ctcattctct tgtttataca
     2041 gatcgtaacc acttgatgta ccagtcttta tcttttttac aactagttcg ctcaactcgt
     2101 ctgccatttt ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat tgataactag
     2161 gatatttttc ttggaacttt ttgacatatt cttctacttg ctgatacata acatccaccc
     2221 ctttgctttt tataatgaaa aaaccatcca gatactagta taaacaatta tctggatggt
     2281 gagcataatg aattattgaa tattcaagac acgcagacta tcttcaacga tttgttttgg
     2341 tgttaaatga taacgttcat aaagctgccc tacaggaacg ctatccgtga attctttctc
     2401 tgcaccaaaa tttaatacgt tcattgaaga atcaccataa taacgactga tattttcgcc
     2461 aaatcctcct gacaaactac catcttctaa tgtgatgact aattcgtgtg tttcttttaa
     2521 tttatctaat gtttctacgt cattttctgt gatcgttcct ggattgatca acgttgcttc
     2581 gattcctttt tttctaaaag cttttactgt ttcttttcct aattcaagaa aaccgcctaa
     2641 agctaaaatt gctacttttc ctccattatt tactgtttga taggctggaa tactataatc
     2701 atttgcgata gctgtaccat gttctacacc atgtgtagga attcggattg ctacaggctg
     2761 cgctgtttgt tcgaacgccc aatctagcat agagatcata tcctcttttg ttgctggagc
     2821 aagatatttg atgttcggga tacttgataa gtaaggaatg tcaaatgttc cttggtgtgt
     2881 tcggtcacta gctgagatcg ttccgccacc gactaaaata atcactggat tctcgttgat
     2941 cgctaaatca tgtgataatt gatcatatgc ccgttgcaag aaagtacttg attggaagac
     3001 gactggacgt actccctgag aagccaaagc agaagcgaaa gtgatagagt gctgttcagc
     3061 gatccccacg tcaaaataat gatctggatg tttttcttgg aatgttttca gtccaaaggc
     3121 gcctggtatc gctgcattga ctgcggcaat aggagctgta cttgtttgca ttttcttatc
     3181 caaataatcc atgatgatca tattaaatgt ttctccagaa gttacttgct ttgcctcccc
     3241 ggtggacaga tcaaatggta catgccaatg atatttcatt ttattatcga ttgccggctg
     3301 ataaccgcga cctttttccg tatggacgtg aagtacaatc ggatgatcaa tatcttttac
     3361 ttcttcaaat aaacggatca ccgtttccag atcgtttcca ttatccacgt aacgataatc
     3421 aaatcccatt gctttaaata ggttattgct gcttttaccg ttggtttgac gaagctcagc
     3481 taagttttta tataaactgc catgattctc atcaatagac atctcattat cgtttactaa
     3541 tacgattaga ttggattgta aaacaccacc attgttcaat ccttcaaaag ctaagccacc
     3601 acttagggag ccgtcaccga taaatgctac cacatttcct gattctttct tcagatctct
     3661 cgctttagct aaacctgttg ctaagctgat agatgtagat gtgtgcccca ctgtaaaata
     3721 gtcatgttta ctttcatgtt ggttggaata tccgctcact tcacggtaat gtcctttttc
     3781 aaaaccattt tttcgaccag ttaaaagctt atgaccataa gactgatggg aaacatccca
     3841 aacgatttta tcctttggtg aatcaaatac ataatgaaac gcaatcgtta attctactac
     3901 acctagattc ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga ttttttggcg
     3961 catctcttct gccaactgtt tcatttcctc aacagataat tttttgacat ctgcggggct
     4021 attgattgca tccaatacat gtaatactgt actcataata cattctctcc tccgtactat
     4081 cttcttttaa tgaaaataga tttgttacac tttatctatt ctactactta gagttgactc
     4141 taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt agatcttcgc
     4201 acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg tagaaggtct
     4261 cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc tgagcaatat
     4321 gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc ttttcagttt
     4381 ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc gcacatagac
     4441 attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca gcgatgcctt
     4501 cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa ctttctgaaa
     4561 caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa gaaagacttt
     4621 tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt ttctttttcc
     4681 actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct gatagaagat
     4741 agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca aattgtaaaa
     4801 aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta ataatctgta
     4861 aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca acttcgaaat
     4921 cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac aaagaaaaaa
     4981 tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc aatccttgac
     5041 agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct ttcagtccaa
     5101 ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag gacttttcat
     5161 cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac tcgttacaag
     5221 cttcttctac atacggattt ttattttcat agccttccca attgacataa cgtactaaag
     5281 ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt ttcaaataat
     5341 cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg ctcaaagaaa
     5401 taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa taacttaaaa
     5461 actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat
     5521 gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta
     5581 gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa
     5641 tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt
     5701 ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt
     5761 tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa
     5821 aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat
     5881 ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga
     5941 atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac
     6001 cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat
     6061 ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga
     6121 cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg
     6181 tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca
     6241 gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat
     6301 aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag
     6361 caagagagcg attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg
     6421 tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat
     6481 ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat
     6541 tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg
     6601 ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata
     6661 acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct
     6721 caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt
     6781 cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc
     6841 caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt
     6901 tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata
     6961 agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca
     7021 ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg
     7081 attactttta caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta
     7141 aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca
     7201 gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg
     7261 gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt
     7321 gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct
     7381 tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc
     7441 gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac
     7501 gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc
     7561 aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga
     7621 taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata
     7681 tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc
     7741 aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct
     7801 aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc
     7861 aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat
     7921 aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat
     7981 aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg
     8041 aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca
     8101 ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     8161 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     8221 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac
     8281 gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat
     8341 ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt
     8401 tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc
     8461 agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac
     8521 cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta
     8581 cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc
     8641 tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg
     8701 gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat
     8761 cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa
     8821 gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca
     8881 tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta
     8941 ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt
     9001 tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct
     9061 ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg
     9121 caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa
     9181 ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt
     9241 tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt
     9301 gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt
     9361 ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga
     9421 aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa
     9481 gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct
     9541 cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa
     9601 aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag
     9661 acagacaatt aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag
     9721 acaaaaaata aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt
     9781 taaagatatt ttttcataag gaataaagaa tacttcatat aaaatatata aaaaagaacc
     9841 gcagcgagtg tcgctacggt tctcaaattt gttcttcatt tcaacataag tttttcttca
     9901 atttgcttat aatttacgtg caaagaatga aacgactaat actaaaatga ttgcaccgat
     9961 caatgatgga atgatagcca ttcctgcaat tgttggtccc caagttccta gtaaagcttg
    10021 tccaattgct gaacctacta aaccagcaat gatgtttgcg atgatcccca ttgatgatcc
    10081 tttattcgtg attgctcctg caattgctcc aatgataccg ccgacgatta atgcccaaat
    10141 aaatcccatt ttaaattcct cctaactgac ttcttacttc ttattgtaca cgtggttcgt
    10201 tattttcttt gactttttgt gcacctttgc ctacagcaga ttttgcttta tctgtttggc
    10261 gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac tttgtcttgt actgtttctt
    10321 gatctttttg gtattcttct tgtgttttga tgtctgcaac attcacattc acttcgataa
    10381 catctaaatg tgtcatattg tttacttcag tactgatcaa ttctttgatt tggttgtaaa
    10441 tatcttcgat gtctttaccg tattctacga tcacatctag atccacagct acttgtttct
    10501 tacctacttc tgtattgata cctgctgtta cgttatctgt gttgactaat ttttcagcca
    10561 tatttgagaa aaatccgcca tctaatgtca atagaccatc tacatctttc aatgcataac
    10621 cgatgatttt ttggatgact ttgtcttcat aagtcaattc accattcatc atgcggtttt
    10681 ctcctttttc atttgccaaa actgatccat ttgttgtatt catgtttttg ttttccatct
    10741 ttgtttcctc ctatgattta aaaaattgat caactattcc tgactgtttc aaataaaaac
    10801 cgattcctgc gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa aatccaatcg
    10861 ttaaccataa aacagctagc agtagtccaa gacccccaaa gataataggg agcttgtatg
    10921 ctgaaaatag ttctttcatt atttggccct cctattccac tcgagagtgc ttgtagtctc
    10981 ttgtatcttc ttgttcttca taaccgctgt atgtgactgc cactttgacc gtttcttttg
    11041 ttccgagtac tttagttact tcttcttcga tatcttgcat caatacacct gttttcccta
    11101 ctaatgaaga agttcgtttc aagtctccgt tgacatggat atggattcga tttttcgttg
    11161 aacgtatccg tactttaggt gaatgaatga actcttcttc gtgtaaatgg gaacgtacca
    11221 acccttcgat cgcttttttg tcgattgtta gtttgccatc ttcccgtttc ataacgaaag
    11281 ttcctcttga ctttggatag aaaagaacga ctagcaagat gataatagca agaacggcaa
    11341 atactacagc tgcccagaaa atatattgtt gcatgtagta attcgttaaa gtaaagaagc
    11401 gaaatcgttc caattgttca ggcataatat acggcgcatt gatcaacgca atcgctagaa
    11461 atactgaaag tagcagtaaa ctcatgataa caccgattat ttttaacccc ttattcattt
    11521 tccacctcct ataaacttat ttttaccctg taaataactc taccgacaat cacagtatag
    11581 atagcgaaca gttagcatag catcagtaaa taaagcgcta ccaaacattg ctggatcata
    11641 acggcgtttt attgccagtc tgatactgaa ataacaattg cttacttatt ctctatttac
    11701 ttttgattgc tggctatact taaaggatat tattcaagat taaaaattgc aaagattatg
    11761 ctcagttatt attacaagat tctattt
//
LOCUS       DAZKAY010000085        11694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000085 DAZKAY010000000
VERSION     DAZKAY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.086"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..586)
                     /locus_tag="ACG8WP_002204"
     CDS             complement(179..586)
                     /locus_tag="ACG8WP_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9028482.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1785)
                     /locus_tag="ACG8WP_002205"
     CDS             complement(775..1785)
                     /locus_tag="ACG8WP_002205"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9028483.1"
                     /translation="MPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVNVDG
                     VLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPI
                     PIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEE
                     QDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSK
                     ILNTNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLT
                     DKNFPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKN
                     PN"
     gene            1911..2129
                     /locus_tag="ACG8WP_002206"
     CDS             1911..2129
                     /locus_tag="ACG8WP_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028484.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2222..2785
                     /locus_tag="ACG8WP_002207"
     CDS             2222..2785
                     /locus_tag="ACG8WP_002207"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9028485.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="ACG8WP_002208"
     CDS             complement(2978..3217)
                     /locus_tag="ACG8WP_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028486.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="ACG8WP_002209"
     CDS             complement(3241..4764)
                     /locus_tag="ACG8WP_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9028487.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="ACG8WP_002210"
     CDS             complement(4782..4904)
                     /locus_tag="ACG8WP_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9028488.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="ACG8WP_002211"
     CDS             complement(4934..5917)
                     /locus_tag="ACG8WP_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9028489.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_002212"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="ACG8WP_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9028490.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="ACG8WP_002213"
     CDS             complement(6111..6488)
                     /locus_tag="ACG8WP_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028491.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="ACG8WP_002214"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="ACG8WP_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9028492.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="ACG8WP_002215"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="ACG8WP_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9028493.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9272)
                     /locus_tag="ACG8WP_002216"
     CDS             complement(7506..9272)
                     /locus_tag="ACG8WP_002216"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028494.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="ACG8WP_002217"
     CDS             complement(9232..10986)
                     /locus_tag="ACG8WP_002217"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028495.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="ACG8WP_002218"
     CDS             complement(11096..11665)
                     /locus_tag="ACG8WP_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP9028496.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3688 a         2256 c         1690 g         4060 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttat
//
LOCUS       DAZKAY010000086        11540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000086 DAZKAY010000000
VERSION     DAZKAY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.087"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..722)
                     /locus_tag="ACG8WP_002219"
     CDS             complement(237..722)
                     /locus_tag="ACG8WP_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9028497.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    812..1157
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1202..1843
                     /locus_tag="ACG8WP_002220"
     CDS             1202..1843
                     /locus_tag="ACG8WP_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9028498.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1862..2497
                     /locus_tag="ACG8WP_002221"
     CDS             1862..2497
                     /locus_tag="ACG8WP_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9028499.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2515..3330
                     /locus_tag="ACG8WP_002222"
     CDS             2515..3330
                     /locus_tag="ACG8WP_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9028500.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3649..5085
                     /locus_tag="ACG8WP_002223"
     CDS             3649..5085
                     /locus_tag="ACG8WP_002223"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9028501.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5274..7721
                     /locus_tag="ACG8WP_002224"
     CDS             5274..7721
                     /locus_tag="ACG8WP_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9028502.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7951..9018
                     /gene="rlmN"
                     /locus_tag="ACG8WP_002225"
     CDS             7951..9018
                     /gene="rlmN"
                     /locus_tag="ACG8WP_002225"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9028503.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9008..9214
                     /locus_tag="ACG8WP_002226"
     CDS             9008..9214
                     /locus_tag="ACG8WP_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028504.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9211..10314)
                     /locus_tag="ACG8WP_002227"
     CDS             complement(9211..10314)
                     /locus_tag="ACG8WP_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9028505.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10866..11414
                     /gene="aac(6')"
                     /locus_tag="ACG8WP_002228"
     CDS             10866..11414
                     /gene="aac(6')"
                     /locus_tag="ACG8WP_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9028506.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3836 a         1949 c         2486 g         3269 t
ORIGIN      
        1 atcaaaagaa taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct
       61 tttttcgtag aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt
      121 ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt
      181 tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac
      241 aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa
      301 tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca
      361 atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga
      421 gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat
      481 aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc
      541 aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc
      601 ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt
      661 tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc
      721 atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa
      781 ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt
      841 taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca
      901 tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc
      961 ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt
     1021 ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga
     1081 acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg
     1141 tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa
     1201 gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa
     1261 attattttta tttaccttga ttggctcatt accattaggg attgttttag cattaggatt
     1321 gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg
     1381 aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt
     1441 ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc
     1501 agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt
     1561 tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat
     1621 tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta
     1681 tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac
     1741 tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat
     1801 cgtaatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa
     1861 tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt
     1921 gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa
     1981 aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt
     2041 agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt
     2101 attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc
     2161 aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc
     2221 ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa
     2281 acagcgggta gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga
     2341 accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt
     2401 gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt
     2461 cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa
     2521 aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac
     2581 aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt
     2641 ttagacgata catttgttcc tatgggattt agagataaag acgggaagct cacaggattt
     2701 gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc
     2761 attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac
     2821 ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa
     2881 aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat
     2941 aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca
     3001 gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa
     3061 gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca
     3121 gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat
     3181 gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc
     3241 aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg
     3301 tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc
     3361 acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga
     3421 atggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc
     3481 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact
     3541 aagcgacttt ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg
     3601 ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa
     3661 gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta
     3721 gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata
     3781 cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat
     3841 aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct
     3901 tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat
     3961 gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat
     4021 tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt
     4081 gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac
     4141 tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat
     4201 ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca
     4261 aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc
     4321 tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa
     4381 aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg
     4441 attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat
     4501 gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg
     4561 acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa
     4621 aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt
     4681 gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt
     4741 cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat
     4801 gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc
     4861 cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc
     4921 tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta
     4981 tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat
     5041 tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa
     5101 caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag
     5161 aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt
     5221 agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac
     5281 aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc
     5341 agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg
     5401 gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag
     5461 gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta
     5521 agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt
     5581 tcggtatctt attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa
     5641 atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca
     5701 ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg
     5761 cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca
     5821 gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg
     5881 aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa
     5941 tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa
     6001 aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg
     6061 atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca
     6121 gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac
     6181 agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag
     6241 aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc
     6301 ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg
     6361 gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct
     6421 taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa
     6481 ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag
     6541 caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg
     6601 tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac
     6661 ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact
     6721 tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa
     6781 gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg
     6841 ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta
     6901 gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat
     6961 tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact
     7021 tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag
     7081 ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa
     7141 tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta
     7201 tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta
     7261 cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata
     7321 tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg
     7381 accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag
     7441 agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg
     7501 agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc
     7561 tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag
     7621 ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg
     7681 aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt
     7741 tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt
     7801 ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag
     7861 ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag
     7921 cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg
     7981 aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa
     8041 gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg
     8101 aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc
     8161 gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg
     8221 attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta
     8281 ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg
     8341 actgctggag aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat
     8401 cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac
     8461 gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct
     8521 cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac
     8581 ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg
     8641 atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat
     8701 ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat
     8761 agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat
     8821 gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa
     8881 gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt
     8941 aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa
     9001 aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt
     9061 tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat
     9121 aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac
     9181 agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct
     9241 ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt
     9301 gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc
     9361 taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt
     9421 cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag
     9481 tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt
     9541 accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac
     9601 caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc
     9661 gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt
     9721 tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt
     9781 agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc
     9841 ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag
     9901 tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt
     9961 cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac
    10021 agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc
    10081 atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc
    10141 gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc
    10201 agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt
    10261 ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat
    10321 ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc
    10381 tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca
    10441 ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca
    10501 ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat
    10561 ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa
    10621 ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct
    10681 ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt
    10741 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
    10801 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
    10861 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
    10921 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
    10981 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
    11041 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
    11101 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
    11161 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
    11221 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
    11281 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
    11341 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
    11401 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
    11461 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
    11521 acttttggaa caccgtgtac
//
LOCUS       DAZKAY010000087        11117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000087 DAZKAY010000000
VERSION     DAZKAY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.090"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..1338
                     /gene="lysA"
                     /locus_tag="ACG8WP_002229"
     CDS             79..1338
                     /gene="lysA"
                     /locus_tag="ACG8WP_002229"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9028507.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1325..2125
                     /gene="dapF"
                     /locus_tag="ACG8WP_002230"
     CDS             1325..2125
                     /gene="dapF"
                     /locus_tag="ACG8WP_002230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9028508.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2128..3009
                     /gene="dapA"
                     /locus_tag="ACG8WP_002231"
     CDS             2128..3009
                     /gene="dapA"
                     /locus_tag="ACG8WP_002231"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9028509.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3006..3722
                     /gene="dapB"
                     /locus_tag="ACG8WP_002232"
     CDS             3006..3722
                     /gene="dapB"
                     /locus_tag="ACG8WP_002232"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9028510.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3728..4423
                     /gene="dapD"
                     /locus_tag="ACG8WP_002233"
     CDS             3728..4423
                     /gene="dapD"
                     /locus_tag="ACG8WP_002233"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9028511.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4439..5440
                     /locus_tag="ACG8WP_002234"
     CDS             4439..5440
                     /locus_tag="ACG8WP_002234"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9028512.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5437..6798
                     /locus_tag="ACG8WP_002235"
     CDS             5437..6798
                     /locus_tag="ACG8WP_002235"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9028513.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6758..7909
                     /locus_tag="ACG8WP_002236"
     CDS             6758..7909
                     /locus_tag="ACG8WP_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9028514.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7884..9053
                     /locus_tag="ACG8WP_002237"
     CDS             7884..9053
                     /locus_tag="ACG8WP_002237"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9028515.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHIKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9228..10607
                     /locus_tag="ACG8WP_002238"
     CDS             9228..10607
                     /locus_tag="ACG8WP_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9028516.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10888..11117
                     /locus_tag="ACG8WP_002239"
                     /pseudo
     CDS             10888..11117
                     /locus_tag="ACG8WP_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3700 a         1945 c         2492 g         2980 t
ORIGIN      
        1 ttattccaat aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt
       61 gaaaagagga atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg
      121 gcagaaaagt ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg
      181 ctgtcattca agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct
      241 aaagcattcc aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat
      301 gttgtcagtg gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc
      361 tacttccatg gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg
      421 ttgcatattg ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc
      481 tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat
      541 gaatatatcg tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag
      601 tgccaacaag cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat
      661 gctcatatcg ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg
      721 acgaattact taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg
      781 attcgttata cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt
      841 gaatatacgg aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa
      901 ccgggtagaa gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa
      961 aagacaccac ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca
     1021 gctctgtatc aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca
     1081 gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct
     1141 tatttgccga aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga
     1201 tactcgatga gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat
     1261 ggccaatcaa gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt
     1321 tcctatgatc cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc
     1381 agaagacaca gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat
     1441 agacggattg atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg
     1501 cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca
     1561 gaacatgatg aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac
     1621 ggtgttggat ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc
     1681 ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg
     1741 tgaaaaagta acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga
     1801 aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt
     1861 caaagaaaaa accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg
     1921 tacctatgaa agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta
     1981 tgtggttgct aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca
     2041 aggagacttg atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga
     2101 atggacaaga atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg
     2161 ccgatgaatg aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat
     2221 ttagatgaac aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc
     2281 acacaatctg aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg
     2341 ccggtgattg caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat
     2401 tttgcttctt tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt
     2461 gatgcaggac tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt
     2521 ttgtataatg ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta
     2581 gcagaacatc ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg
     2641 gatgccgctc ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc
     2701 ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct
     2761 aaaatagtcc atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa
     2821 aagcaattaa atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct
     2881 gtcaaagcag ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt
     2941 tctctttcaa aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt
     3001 gaaaaatgaa aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg
     3061 cacaagaaag aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc
     3121 ttgcttttac ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga
     3181 aaatcctttc gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata
     3241 cacaatcaca gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg
     3301 ctaatttttc gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt
     3361 tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat
     3421 ctggtactgc aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat
     3481 acaactggca agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag
     3541 gttctttacc tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca
     3601 aacatgaatc tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt
     3661 tgaaagatca gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt
     3721 aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc
     3781 tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg
     3841 tgaaggaagc agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc
     3901 tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc
     3961 tttgctagat attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca
     4021 agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt
     4081 aatcggtgaa aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg
     4141 caaaaactgc catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc
     4201 caaaccggtt atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg
     4261 tgtccatatc ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc
     4321 agcagaaact gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac
     4381 aaaagaaaat acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat
     4441 gcgcgaaata gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa
     4501 agtgttagaa gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc
     4561 agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac
     4621 ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt
     4681 tgcgccaatc gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga
     4741 agattcggat attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg
     4801 gctgattgca aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca
     4861 agaagccttt ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg
     4921 gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc
     4981 ttataatatt agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac
     5041 aaaagaagaa cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc
     5101 agtatcagca acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt
     5161 tcaactgcaa aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg
     5221 gattcaatta gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg
     5281 aacagatatg gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt
     5341 gttctatacg actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc
     5401 tgcagcttta atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg
     5461 aaattcggcg gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt
     5521 ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat
     5581 gatgacaaag taacagactt actttatttg atttatgctt accttcagca tcatgttgat
     5641 tggacacccc tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc
     5701 ctgcagttac ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag
     5761 ataactgaag attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag
     5821 tatcttggtt accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa
     5881 atcgatcagg aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg
     5941 gttgttccag ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg
     6001 ggaagcgaca ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat
     6061 tggacggatg tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc
     6121 atcgaggaaa tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt
     6181 catgaagcag ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc
     6241 aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga
     6301 ctgacaggaa tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg
     6361 tccgatgaaa tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca
     6421 atcgaacata ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt
     6481 gccgataagt tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc
     6541 gtcgtgatcg aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt
     6601 ggattatctg ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta
     6661 tcacaaggag cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca
     6721 gtagtgaagg gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc
     6781 actccatcaa attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga
     6841 acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta
     6901 ttttgataat cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt
     6961 caaagaagaa acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg
     7021 tcatgacgga catatggcga tgcttttagg gttgactgac tttataacta aacacattga
     7081 ggatcttatt tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc
     7141 acaaagcgtt attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt
     7201 tcatctatgg cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc
     7261 acaaagcagt gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga
     7321 aaaaggaatc gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga
     7381 ccggcaatta ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac
     7441 gatccgaaat gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc
     7501 agatatgcaa aattatttga agaggggatt agaccattta gcagaacagt ttcactctac
     7561 agctactatt tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt
     7621 atatgcagca ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc
     7681 cgaagatttt ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg
     7741 tgatacccct gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg
     7801 tgtagaatgg tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag
     7861 ttcaaaagag gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt
     7921 ctagaataga agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg
     7981 tgatcaaact caccctggga gaaccggatt ttccaactcc tgaacatatc aagcaagcag
     8041 gaatagcagc gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac
     8101 tgcgggaagc ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa
     8161 cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt
     8221 tggaagaagg agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag
     8281 tggaactagc tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc
     8341 agccggaaca atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt
     8401 taaactatcc taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg
     8461 ctgaggtttt aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga
     8521 cttatagtgg aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa
     8581 gcgggttatc caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa
     8641 aagcccttat tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga
     8701 tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga
     8761 aagagcaata tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg
     8821 aagtgaccca gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa
     8881 ctgactcttg gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag
     8941 gaattgcatt tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg
     9001 aaaaattaca tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag
     9061 attttatcaa aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt
     9121 ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt
     9181 aaattgttta gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca
     9241 aagttgtcat tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac
     9301 gatacggtga aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt
     9361 catgtggtat ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt
     9421 tcagaccaga aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa
     9481 cgatcgatgc cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag
     9541 aatatcctta tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac
     9601 caggagcaga tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca
     9661 aagaaaaatt aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg
     9721 ggatcgaagc agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga
     9781 tcgatagacc tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc
     9841 taaatgaaaa cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg
     9901 gaaaagttga ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag
     9961 ctgccggtgt gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg
    10021 gctggatcca aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag
    10081 atgctacact tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg
    10141 ttgcacgtag agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac
    10201 cgttcggcgg agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa
    10261 gcggattgaa tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg
    10321 aagatacgtt acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc
    10381 aattattctc ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg
    10441 atgttacagg tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag
    10501 aactagcaga agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga
    10561 atctagccgc tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat
    10621 ttgaaaagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    10681 cagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    10741 cgtttatttt caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac
    10801 caaaaaatct aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa
    10861 attaaagatc gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat
    10921 caagagattg aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta
    10981 cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc
    11041 ttgatcaaga agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat
    11101 tcttatcgag gaaaagt
//
LOCUS       DAZKAY010000088        11055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000088 DAZKAY010000000
VERSION     DAZKAY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.091"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..851
                     /locus_tag="ACG8WP_002240"
     CDS             72..851
                     /locus_tag="ACG8WP_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028517.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            897..1841
                     /locus_tag="ACG8WP_002241"
     CDS             897..1841
                     /locus_tag="ACG8WP_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9028518.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1857..2636
                     /locus_tag="ACG8WP_002242"
     CDS             1857..2636
                     /locus_tag="ACG8WP_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9028519.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2648..3346
                     /locus_tag="ACG8WP_002243"
     CDS             2648..3346
                     /locus_tag="ACG8WP_002243"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9028520.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3374..4138
                     /locus_tag="ACG8WP_002244"
     CDS             3374..4138
                     /locus_tag="ACG8WP_002244"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9028521.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4182..5633
                     /locus_tag="ACG8WP_002245"
     CDS             4182..5633
                     /locus_tag="ACG8WP_002245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9028522.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5665..6801
                     /locus_tag="ACG8WP_002246"
     CDS             5665..6801
                     /locus_tag="ACG8WP_002246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9028523.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6801..7907
                     /locus_tag="ACG8WP_002247"
     CDS             6801..7907
                     /locus_tag="ACG8WP_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9028524.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7913..8935
                     /locus_tag="ACG8WP_002248"
     CDS             7913..8935
                     /locus_tag="ACG8WP_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFP9028525.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8957..10012
                     /locus_tag="ACG8WP_002249"
     CDS             8957..10012
                     /locus_tag="ACG8WP_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9028526.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10630..>11055
                     /locus_tag="ACG8WP_002250"
     CDS             10630..>11055
                     /locus_tag="ACG8WP_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028527.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVS"
BASE COUNT         4046 a         1523 c         2242 g         3244 t
ORIGIN      
        1 aaaaagctgt catttttatg taagtgaatt atatcttaat ttatctatca tcaatactta
       61 ggagtgaatc tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag
      121 gtgctattgc gatctgtttg tttggatacc atatcaataa tcagcggcaa catcagcaac
      181 ggatcaatta tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa
      241 aagaagtaga caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag
      301 aagaaacaat cacaagtttg tcaaataagc tatcctctgt caaactttca gcagatgatt
      361 acgacatcaa agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag
      421 caatcgtgac acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg
      481 atttatttac taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag
      541 aaaaggtgac aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata
      601 gtccttggaa aacggttgtt atgcaatatc taggattcgc tgatattcag atcgcgcaag
      661 tcacgcaact agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg
      721 ctacttacga tcaatatttg acggtgctca gtcaaataga gcagatccgg aatgaaaaaa
      781 taagcgcagc atacgcaaca aaagcacaaa ctgttgctga gcaaatgggc tatagcaata
      841 taagctattg acagttaaaa cgttgattca agcaaggtta gcaaacggag gaaactatgg
      901 caagacgaag aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta
      961 tcgtattagt attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt
     1021 ctattgagaa aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg
     1081 atctggacaa acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag
     1141 gtcgggtcga tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca
     1201 aacagacaac gatcgtcagc attcctcgtg atacgtatgt cgacatcgta ggtcgtgaca
     1261 caaaagacaa gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg
     1321 tacaaaaata tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa
     1381 aggaactcgt tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag
     1441 atggctatga ctttccaatc ggtaaaatta cattgaacgg ggatcaggca ttatcttatt
     1501 ctcgtatgcg ttatgaagac ccaaatggtg attacggtag acaagggcgc caaagaaaga
     1561 tcgtagaagg cgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg
     1621 ttttgaatgc tctagagtcg aatatgaaaa cagacatgaa ctttgatgaa atgaaaaaaa
     1681 ttgctttaga ttatcgggag gcatttggta cgattaagca agatcagctg caaggagaag
     1741 gcttcatgca agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtga
     1801 aaaaagaatt aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg
     1861 aagagacaat cagcttgaaa gaaatttttg aaacattacg caaacacttg gtaataatca
     1921 tcatctcaat gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac
     1981 caaaatacag ttcacaagca caattgatcg tgacactgcc acaatcagac actgcaaatg
     2041 tcaatgatgt caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg
     2101 atttagtgct caatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg
     2161 ctgaacaaat caaagatgcc atctcagtta atcaggcaga aaattcacaa atgttctcga
     2221 ttcaggcaat aagtacgaat gcagtggctg ctcaacagat cgctaacaca acttcacaag
     2281 ttttccaaga gaacgcaaaa gatgtgatga atgttgataa aatctcgatc atttcaggtg
     2341 cagttgccaa tacgacacct gtttcgccaa ataataaatt gaatttaatg actggcttaa
     2401 tgttaggaat gatggtaggt gtaggtctgg cattcttatt ggaattactc gatcggacag
     2461 tgaaagatga aaaatatgtg acagatacat taggattccc aatcttagga acagttccag
     2521 aaatgtcagc gaaagaattg aatgctggta tccgtaaaaa aggaccagct cagacacaac
     2581 caaaagctac aaacgataaa acagcgccat cacgcagaag ccgctcaaga gtctaggagg
     2641 aaagaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
     2701 cacactcacc aatccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
     2761 ccagtttgcc tccgcagctg gtcagcaaat caaaacgatt gttgtgactt catcaggacc
     2821 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
     2881 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
     2941 gaacaacgca agcggattaa gtactgttct tagttcatca ggaagtgtag cagatgagat
     3001 ccaacgaaca ccagtcgaca atttgtcgat tctgccaagt ggacctaaac caccaaatcc
     3061 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
     3121 tgatgtagtg atcttcgaca tgccgccagt cgttgccgta acagatgcac agatcatgtc
     3181 ttcaaaaacg gatggcacct tattggtcgt acgggaaaat acgtcacgaa aagaatcatt
     3241 gactaaagca aaagaactgt tagatatggt acaagcacga gtcttaggtg tcgtatacaa
     3301 cggagcagaa cacagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga
     3361 ggagacaaga gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacggggc
     3421 agaaaactta gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcattacgca
     3481 tattttatgc acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat
     3541 ttctctagtc gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt
     3601 ggaaggacaa gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat
     3661 tttatttaca gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc
     3721 gttatatgca gaacggcttt tttttgagtt gttagaacta ggaaagatac cagtgatcgt
     3781 ccatccagaa cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga
     3841 tatgggatgt ttggcccaac ttactgcgcc aagttatgtt ggtgtatttg gcaaagatat
     3901 ccagaaaaca gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc
     3961 acacggggtc tctaagcgca ccttttactt aaaagagtgc tatgagcaga ttgccaagga
     4021 gtttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
     4081 tgagatcaat tatccaatct atgaagaggt aaaaaagaag aagtttggtt tattttagtt
     4141 ctttattcgt agataaaaag gaaatgagga gagagtgaga gatgtataga aaaaactcaa
     4201 gtggttggtc taaacatatt gattttattt tattagactt attttgtgtc cagcttgctt
     4261 tttatatatc atatgtaatg cgacagggtg actggaatcc gtatgtgatt cctatttatc
     4321 gccacatggc aatttttata gaacttgaga atcttacgat tatattttta tttgaggcat
     4381 ataaaaatgt tttaaaaaga ggatattata aagaggctgt agcttctata aagcaatctt
     4441 ttatgctgtt acttgtgtgc agtttatacc ttattacaat gcaggatgga aatgattatt
     4501 ccagagtttc tttatttata atgggggtgg tgtatggatt attaacatat ataattcgta
     4561 tactttggaa agggttgttg catcaccgca tgaaaaacgg tgaagatgta tcgttagtta
     4621 ttgtaacatc tcaaaagatt gcaaaaaatg tagttcgtaa tcttaaagag aagaattatg
     4681 gcatgtataa acttagtggt gtgattgtag tagacagaga tatgtatgga actaccatcg
     4741 gaggtgtccc agtagttgca aatgctgata atgcagcgga atatctttta cagaattggg
     4801 tagatgaagt attcatcaat gttgacgaaa gtgttccata tcccaaagaa ttaatcgcca
     4861 gatgttcgga aatgggactt gctgtacata tgaatcttac aaaagtgtct gatagtacac
     4921 gtggaaaaca atttattggc cgagttggtg attatactgt tattacatct agtattaatg
     4981 ttatgacgat gaggcaagca tttataaaga gaacgattga cattcttgct ggaattattg
     5041 gatgtattgc aacaggtgtt atttttatct tcgtagcacc agttatttac atcaaatcac
     5101 cagggccgat tttctttgca caagaacgta ttggtcaaaa tggtaagaca tttaagatgt
     5161 ataagttccg tagtatgtat atggatgcag aagaacgcaa agcagagttg atgaaagaaa
     5221 ataagatgag taataatctc atgtttaaga tggattttga cccaagaatc attggcaata
     5281 aaatacttcc tgatggaaca aagaagacag gaattggaca atttattcga tctacatcat
     5341 tggatgagtt tccacaattt tataatgtcc taatgggaaa aatgagcctg gtggggttta
     5401 gaccttgctt gaagtcagaa tatgagaagt atgattttca tcatcgtgcg agaattgcga
     5461 tgaaacctgg gattactgga atgtggcagg tgagtggtag aagtgatatc acagacttcg
     5521 aagaggttgt aaaacttgat gcagaatata tcaggaattg gagtatggga ttagatttta
     5581 gaattttgtt taggacagtc aaaactgtgc ttcatagaaa cggttccatg taagataaag
     5641 atactataga aggagtaaaa cgtgatggca agaaaaaaat taaaaatctg tatgctaggt
     5701 cataaaagaa ttccatcaag agaaggtgga attgagatag tggtagaaga attggcaacg
     5761 cgaatggttg cacttgggca tgaagtgact tgctataatc gaagtggtca tcatgttagc
     5821 ggaaaggaat ttgaccagca taaaaataaa gaatataaag gtgtaaaatt aaagactatt
     5881 tttacattaa atataaaagg aattgcagca atgagttctt cagtatttgg tggaattaga
     5941 gcagcattcg gaaaatatga tattgttcat tttcatgcag aaggaccatg tgctatgttg
     6001 tggttgccaa aactttttgg gaaaagatgt gttgcaacaa ttcatggtct agatcatcaa
     6061 agagaaaaat ggaacaagct tgctagtaca tacatcatgt taggggaaaa gtgtgctgta
     6121 aaatttgcag atgaaataat tgtacttagt gaaagcgtcc aaaattattt tgaagatata
     6181 tatggaagaa aaacaaggtt tataccaaat ggcgttaaaa aaattgaaat aaaatcagca
     6241 ggattgatta cagaaaagta tggattaaca aaagattctt atattttgtt tttggggcga
     6301 ttggttcccg aaaaaggaat tcggtatcta attgaggcat ttaaagatgt tcagacagat
     6361 aaaaaactta taatagccgg aggaagctct gatacagatg aatttgcaaa tgaactaaag
     6421 gaattagcaa aaggtgatga gcgaattata tttactggat ttgtacaagg acaggaatta
     6481 gaagaattat atagtaatgc gtatatctat acacttccaa gtgatttaga aggaatgcca
     6541 ttaagtttgt tagaagcaat gagttatggg aactgttgta tagtttcaaa tatctcagag
     6601 tgtacggaag tagtagaaga caaggctatg atttttaaga aatcggatgt aagtgattta
     6661 aagataagat tagaagaggc atgcaatcaa tcggaaatgg ttaaagtttt gaagaatcaa
     6721 gctactgagt ttatttgtag taaatataat tgggataaaa tcgtacaaga aacattaaat
     6781 ttgtataggg gaaatatata atgattcgaa tattacagtg tgtgaatgat atgcatcgtg
     6841 cagggttgga gacgatgcta atgaactatt ataggaatat agatcgaact aaaattcagt
     6901 ttgatttttt gacacatcgt ccatataaaa gtgattatga tgaggaaata ataagtcttg
     6961 gaggaaaggt ctattatgca ccgagacttt atccacaaaa ttatcctaaa tattttaaat
     7021 ggatggaaca attttttaag gatcatccag aatatgagat tgttcattct catattgatg
     7081 cgatgagtta tttaccttta aaagctgcaa aaaaagctgg gattccgata cggatagcac
     7141 atagccataa tacatcgata gatagagatt ttaaatatct tttaaagcaa tattatcgat
     7201 gtaagttaac gtctgttttg acacatgaat tttcatgtgg aaaagtagca ggtgattttt
     7261 tgtttcgaaa tgataaattc actttgattc caaatgcagt ggatgctaaa aaattttatt
     7321 atgatgaaag tattagaaat gatgtacgaa tgaaattagg aatttcaagt cagacgtttg
     7381 taatgggaca tattggaaga atttcatatt ctaaaaatca tcgttttttg attgaaattt
     7441 ttaaagaatt tcacgctatt aagaataatt ccgtattatt aattattgga actggtgata
     7501 tggaagaaga aataaaaaat tatgcaaaga aaagtggcat agatgatgat attaaatttt
     7561 taggaaatag aaatgatata gaaaaatttt atcaagcatt tgacgtatta atgttgccat
     7621 cattatttga aggggttccg ttagtgggta ttgaagctca atttgcagat ttaccgtgtt
     7681 tcttttcgga gaaagttcct acagaagttg ctttcagtga taagaccaat ttcataagtt
     7741 taaatcaatc ggcaaaagaa tgggcgagag agattgcaaa tgtagatatc tctcatcgag
     7801 attcagaacg ttcgatttta ataaaggcag attataatat tcaaacagca tacaaaatct
     7861 tagaatcaaa atattatgag ttatttgaat taatacaaag aggttaaaga atatgacaca
     7921 gaaacattca agtataaaaa gaattataca tcgcatatcc gaagaattga atgcaacaag
     7981 acgaattgca aagataacat cttggaagga agcatttgcc acgttacaag ctaaaattga
     8041 tattcaagtc atgaatcgta atggatataa tgaaagtaaa aaacagaagg aacatttatt
     8101 aaaaaaacat gaggtaatgc tgaagtatta tgaaaagact ttttcagatt ttttgaaaac
     8161 atatgattat agtcaaaatc aaataattaa tcaatcaaaa tcaccatatt ctaactgcat
     8221 ttgggtttgt tggtggcagg gattggatca atcgccggaa attgtaaaag cttgtgtaaa
     8281 atcaataaaa cgaaatgcag ggaaacatac tgttataatt ttaacagaag ataattatag
     8341 acaatatgtt gatataccaa aatgggtgga agaaaagaaa aacaaaggaa tcattacgag
     8401 aacaaattat tctgatttat taagattatc tttgctagca aaacatggag gtttgtggtt
     8461 agacgccaca ttttattgta caaaaccaat accagatgag tattttgata tcgatatttg
     8521 gtctattagg aggcctgatt atgctcacgc atccgtagca tcaggatatt ttgcgggtta
     8581 ttcattagca tgtaatcaag agtctagatg gatgtttgct ataattagag actttttttt
     8641 aaattattgg aaaaataatg atatgatggt ggattatttg atgatagatt atatgattgt
     8701 tttagcacag agaataaatc atgaaatagc acagacattt gataaaatac ctagtaacaa
     8761 tccattatgt gacgaattga ttaaaaattt aaataatata tatgatgaaa aagtatggaa
     8821 cttattaaag aaagatactt tactgtttaa gttgacatgg aaacaatctt ttaaaaagca
     8881 aaaagatgat aaagttactt tttatgggat gttggtagaa aacaaattat tataaatatt
     8941 agaggtataa aaagttatga aacacaaggt aagtgtgata gtaccaattt acaatgttga
     9001 aaaatattta agaaaatgtg tggatagttt attgagtcag acattaaaag agatagaaat
     9061 cgtattagtt gatgatggtt cgccagatgc ttcgggggaa atagcagatg agtatcaaaa
     9121 aaaatattct aatgtaaaaa caatacatag agaaaatggg gggttaggac cggcaagaaa
     9181 tacaggaatt gagaatgcga caggagaata tgtggctttt cttgattcag atgattgggt
     9241 agaaagtgat atgtatgaaa aattatactt agttgcgtcc aaagaaaatg ctgatatagt
     9301 agttagtgga cattgtgatt ttgcgaatga caaagctatg gtgaaaaagc aacatccttt
     9361 gagtgggaat gtatatgata cgcctaatag tattaaaaat gtacgaaaaa atttatttgg
     9421 acatagtcca aaagatcaag aagtagaggc cttcccgatg tcagtatgta tgtctttgta
     9481 taataaaaat ctgctagata aatatagact aagatttgaa aaaattttat ctgaagatac
     9541 aatttttaac ttgaatgcat atgatgtcgc aaataaaatt gcttttacag aatatacaga
     9601 ttattgttat agaaaagaag aacaagagtc tattacaaaa agtttatctc caaataagca
     9661 gacggaattt aaagaatttt taataactct aaaagctata gcatcgaaag aagatttaga
     9721 atgtcaaatc agagcaaaac gtatggcaat tgattattgt aggctttatg tgggcttagt
     9781 tgatgatgca aatttgggaa ttaaagagaa aagaaagtat ataaaaaaat ttattgaaga
     9841 tgagggatta attcaatgtt ggaagggata tcctacaaat acgctaccat ttcaacaaaa
     9901 attttttcat aaaatgcttg aatgtaagtg ttatacaata ttgttgttgt taagtagaat
     9961 tagaaagata attaaaaata tgaccagaaa aaaattgttt ataatattat aaactaaaag
    10021 tatatcaaat aatagaaatt aacaagttac aaaaaaatat tataaagaat aatattttct
    10081 aaaaagtaaa ctatagtaat gtggattatc accttccaac agtatataaa atgagcgtaa
    10141 aaacggctat ttttgcctta acaacgattt ttttgtatac aaatacgata tgctcattaa
    10201 ttattggaat ttgtagtgct gtttaaatta atgaaggtgt aattatatga gtgatcattt
    10261 tgaaaaagtt tgttgagaag atgtgcttct agtcatgtga tgggagaaaa taatttaact
    10321 gttagagtga atagttaaat tattctggtt atatgaaaaa agtagtttct aaaataatct
    10381 tgcagggatt aacttaaaga aattgagaga atagtttctt tgaaatttct atatacaatg
    10441 agaagctgaa atttactttt atttgatgac tggaaataga aattaaatat attgtattgt
    10501 tacattaaag aaaacttgta tggaaaaatc agtacaatac cattatttaa tgttcttcac
    10561 ttaaagcgca aaaaatatca gtttattaaa ctcgtatata gaacaaatat atgtagacgg
    10621 ggcgaaacga tgagtgtaaa aaaaatgcta tttatggtaa gtacaataat tgtattaaca
    10681 tcccgatttt ggggaattaa tactagttta tctatgcgat attttattat ggcaatatgg
    10741 gatttatatt ttatgttaag ctatttttct tatcgtgaga aaacatataa aaaatacgtt
    10801 atcaagaata acttttggtt gactataatg ccgtttgtga tattttctat ctatactcta
    10861 ataatatggg gaattggttc aaatgcagaa tttgaaaatt ttataaagtt atgttccacg
    10921 ttgctttatt taattcttgc atatggttac gcatgtacag cattttactt atttaaggca
    10981 gaaggaattg attttatttt ttggtcaggt gttatttcat atatgagtgg ttcagttttt
    11041 tatttaattg tatct
//
LOCUS       DAZKAY010000089        10578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000089 DAZKAY010000000
VERSION     DAZKAY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.092"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8WP_002251"
     CDS             33..656
                     /locus_tag="ACG8WP_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028528.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8WP_002252"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8WP_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9028529.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8WP_002253"
     CDS             2152..3033
                     /locus_tag="ACG8WP_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028530.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8WP_002254"
     CDS             3036..3806
                     /locus_tag="ACG8WP_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028531.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8WP_002255"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8WP_002255"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9028532.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8WP_002256"
     CDS             5117..6280
                     /locus_tag="ACG8WP_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9028533.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8WP_002257"
     CDS             6397..6807
                     /locus_tag="ACG8WP_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9028534.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8WP_002258"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8WP_002258"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9028535.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8WP_002259"
     CDS             9380..9664
                     /locus_tag="ACG8WP_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028536.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8WP_002260"
     CDS             9824..10060
                     /locus_tag="ACG8WP_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9028537.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8WP_002261"
     CDS             10057..10455
                     /locus_tag="ACG8WP_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9028538.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3691 a         1770 c         2235 g         2882 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttg
//
LOCUS       DAZKAY010000090        10345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000090 DAZKAY010000000
VERSION     DAZKAY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.093"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1958)
                     /locus_tag="ACG8WP_002262"
     CDS             complement(372..1958)
                     /locus_tag="ACG8WP_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9028539.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            2209..2433
                     /locus_tag="ACG8WP_002263"
     CDS             2209..2433
                     /locus_tag="ACG8WP_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9028540.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2575..4326
                     /locus_tag="ACG8WP_002264"
     CDS             2575..4326
                     /locus_tag="ACG8WP_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9028541.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            4326..6110
                     /locus_tag="ACG8WP_002265"
     CDS             4326..6110
                     /locus_tag="ACG8WP_002265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028542.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6568..7419
                     /gene="licT"
                     /locus_tag="ACG8WP_002266"
     CDS             6568..7419
                     /gene="licT"
                     /locus_tag="ACG8WP_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9028543.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            7516..9402
                     /locus_tag="ACG8WP_002267"
     CDS             7516..9402
                     /locus_tag="ACG8WP_002267"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9028544.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9443..10345)
                     /locus_tag="ACG8WP_002268"
     CDS             complement(9443..10345)
                     /locus_tag="ACG8WP_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9028545.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         3263 a         1860 c         2184 g         3038 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc
     2041 taattttttt tcgtggcgtg tcttgctggt taatgaaaga aattttgttc ttctctaaaa
     2101 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     2161 taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat ggtatcaaca
     2221 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     2281 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     2341 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     2401 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact ttttgcgggc
     2461 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac tattcagcaa
     2521 aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag taaaatgtcg
     2581 atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt aattgggatt
     2641 ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat tgggatcatc
     2701 attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg gatcttatta
     2761 ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac gaatatttgg
     2821 ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca ttttacagaa
     2881 atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca tgcaacgaat
     2941 gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     3001 atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     3061 attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg gtcaaaactc
     3121 cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     3181 agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctcgcggat
     3241 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     3301 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     3361 ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc atttattagc
     3421 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     3481 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     3541 gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc tatttcctct
     3601 tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc gatccaagaa
     3661 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat catcaaatta
     3721 ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa
     3781 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga tcacttcttg
     3841 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     3901 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     3961 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     4021 tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga tgcactttct
     4081 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     4141 aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt
     4201 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcattaggc
     4261 ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga aggaggcgag
     4321 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     4381 agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     4441 ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
     4501 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     4561 ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc caatggtatt
     4621 tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta tttgaaaagc
     4681 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact gtttctcgag
     4741 tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg gtattgactg
     4801 ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga gaaattgcct
     4861 tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     4921 gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     4981 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     5041 aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc aaaacaaatt
     5101 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
     5161 ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc tatgcattta
     5221 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcacct
     5281 ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca gaagaatatg
     5341 caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc gagtttcgca
     5401 atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct tttgtcgcta
     5461 atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc tcgatcatca
     5521 acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat gatcatgaca
     5581 ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg caagatgcct
     5641 tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact atcacagacg
     5701 aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat gaattaccaa
     5761 aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga caacgacagc
     5821 tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg gatgaagcga
     5881 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca aaaatgcgac
     5941 aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat gcagaattga
     6001 ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca cttctagcgc
     6061 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg
     6121 tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga
     6181 tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt atattagaag
     6241 ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata aaacagagct
     6301 tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa aaaaatcacg
     6361 acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt gcttgctttc
     6421 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     6481 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     6541 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     6601 gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     6661 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     6721 aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     6781 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     6841 tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     6901 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     6961 atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     7021 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     7081 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     7141 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     7201 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     7261 taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     7321 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     7381 catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt
     7441 aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     7501 ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc
     7561 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     7621 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt tgtgacggta
     7681 atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa
     7741 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     7801 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctattcta
     7861 gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg cgcatttgac
     7921 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     7981 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     8041 ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     8101 gaagcaccac tgactaccat attttctggc actatttttg aagcacctat ttatcaaaca
     8161 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     8221 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     8281 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg ttttttactc
     8341 gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     8401 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctagtgatg
     8461 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     8521 caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttactgca
     8581 ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc cattgtttct
     8641 ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata
     8701 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt aatgaattta
     8761 acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat tagaccagat
     8821 ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat ggtggtatcc
     8881 tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga tagacagaaa
     8941 agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca
     9001 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     9061 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
     9121 gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact
     9181 gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa
     9241 ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa
     9301 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa tcaaggggag
     9361 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat
     9421 ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt
     9481 tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga
     9541 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
     9601 aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa
     9661 gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta
     9721 aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
     9781 ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct tcatagaacg
     9841 attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga
     9901 aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa
     9961 gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa
    10021 tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg
    10081 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    10141 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    10201 gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg
    10261 ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt
    10321 ctttgatttt ttcacgtaat aacat
//
LOCUS       DAZKAY010000091        10107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000091 DAZKAY010000000
VERSION     DAZKAY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.094"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..801
                     /locus_tag="ACG8WP_002269"
     CDS             322..801
                     /locus_tag="ACG8WP_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9028546.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            834..3062
                     /locus_tag="ACG8WP_002270"
     CDS             834..3062
                     /locus_tag="ACG8WP_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028547.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3191..4834
                     /locus_tag="ACG8WP_002271"
     CDS             3191..4834
                     /locus_tag="ACG8WP_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9028548.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4907..5452)
                     /locus_tag="ACG8WP_002272"
     CDS             complement(4907..5452)
                     /locus_tag="ACG8WP_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9028549.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5475..5960)
                     /locus_tag="ACG8WP_002273"
     CDS             complement(5475..5960)
                     /locus_tag="ACG8WP_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9028550.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5950..6762)
                     /locus_tag="ACG8WP_002274"
     CDS             complement(5950..6762)
                     /locus_tag="ACG8WP_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9028551.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6901..8433)
                     /locus_tag="ACG8WP_002275"
     CDS             complement(6901..8433)
                     /locus_tag="ACG8WP_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9028552.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8542..9573)
                     /locus_tag="ACG8WP_002276"
     CDS             complement(8542..9573)
                     /locus_tag="ACG8WP_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028553.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3370 a         1858 c         1940 g         2939 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagccaaaa
      121 tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat
      181 ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta
      241 taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt actaaaaata
      301 aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc
      361 gttgtagaag actatgataa agttgatgga agaaatgcat taaatatgga tgcaaaaggg
      421 ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta
      481 tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt
      541 ttagatttag cgatcttagc tgcgcaagga agtttatact tccaagatgc ttatcgttat
      601 gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga
      661 atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac
      721 gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt
      781 ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga
      841 atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga
      901 aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga
      961 attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg
     1021 ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga
     1081 cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt
     1141 ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag
     1201 ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat
     1261 tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt
     1321 acccttctga aatcgttcga gcgattgaaa aaggctattt attagagatt caagagccga
     1381 ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga
     1441 tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta
     1501 caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac
     1561 atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa
     1621 ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg
     1681 aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg
     1741 caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata
     1801 aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat
     1861 tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc
     1921 atacaaaaga aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg
     1981 ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg
     2041 gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag
     2101 aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc
     2161 aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata
     2221 aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc
     2281 gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa
     2341 tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa acagagacgt
     2401 accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt
     2461 tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac
     2521 ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag
     2581 cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata
     2641 cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg
     2701 gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg
     2761 gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt
     2821 tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa
     2881 aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg
     2941 cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag
     3001 atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
     3061 gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta
     3121 ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga
     3181 gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta
     3241 tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga
     3301 aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca
     3361 atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt
     3421 cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt
     3481 gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa
     3541 aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa
     3601 agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac
     3661 tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga
     3721 gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg
     3781 atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga
     3841 gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa
     3901 acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga
     3961 agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca
     4021 tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacatgc
     4081 attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa
     4141 ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat
     4201 gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca
     4261 aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt
     4321 agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt
     4381 ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga
     4441 acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac
     4501 gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat
     4561 gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca
     4621 tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat
     4681 ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg
     4741 gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc
     4801 ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt
     4861 ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc
     4921 taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg
     4981 ttttcaaacg aggtctgaca ggtacttgga catgcttgat atgcataccg attgatgtgt
     5041 caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact
     5101 ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa
     5161 tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt
     5221 cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga
     5281 tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc
     5341 ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt
     5401 cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa
     5461 accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata
     5521 ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact
     5581 ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag
     5641 cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca
     5701 acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa
     5761 atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa
     5821 agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt
     5881 acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc
     5941 gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag accagacact
     6001 tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact
     6061 ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga
     6121 aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc
     6181 ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc
     6241 tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc
     6301 tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt
     6361 aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat
     6421 cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt
     6481 tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt
     6541 gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa
     6601 agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc
     6661 gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc
     6721 agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa
     6781 taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat
     6841 ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag
     6901 ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc
     6961 tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg
     7021 gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg
     7081 ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc
     7141 taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg
     7201 atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca
     7261 atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc
     7321 tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc
     7381 tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt
     7441 tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata
     7501 tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg
     7561 gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg
     7621 ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg
     7681 ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat
     7741 aggaaatccc atggacatca aagaagagat accaatattg aatctggaat gcactggaga
     7801 gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt
     7861 cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc
     7921 agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa
     7981 agcaggttgt tccattaaat aaagaatagg aatctgtttt gattttgttc catttgaaat
     8041 ttctctcact cgcttttttg tctcatcatc aaataaaatg aaagggtctt ctaaacgatc
     8101 gataaatact tccattgttg ttgttggtag atgataagaa gtcatgacag gcactgcacc
     8161 aaggtaagac acagaaacag ctagaaggta tgtgtcaaaa gcggaactct tataaataat
     8221 aactttgtct ccggatttca ctccaagatg agccaattga taagcccgtt tcaacacact
     8281 ttcgcagcat tttttgtaag ttgtttcaag tcctaattct gggaaagctg aaagtggctc
     8341 atcaaagata atcggggtct cggaaaagtt agctgcagct tcttgaaaat tcgtaaataa
     8401 gttcaaaggg gaatacgtta tggtctccat cattcacact ccattatctt ttgttaagta
     8461 atatacaaat tcaagataat cgtaacataa tcctttatat aataacatag tttattgtga
     8521 aaaaaagatc atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg
     8581 ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg
     8641 attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg
     8701 atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat
     8761 ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt
     8821 ttcgtccgta ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt
     8881 tggtattggt actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt
     8941 caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag
     9001 aagaatggca gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct
     9061 gcttgtttga aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc
     9121 atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat
     9181 ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg
     9241 cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt
     9301 agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt
     9361 ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc
     9421 agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg
     9481 attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt
     9541 ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt
     9601 ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag
     9661 caaattttta actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat
     9721 tttattagtg cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg
     9781 gttacttcat gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt
     9841 tatttccgaa aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata
     9901 atggtttgtc taacgatatt gttgggataa ttcttaaaga agtattagaa atagttggta
     9961 cttcattact ttacatgaag atttattcta tggtaggagc aattatattg agaattaatc
    10021 cttttttata taagttgttt gcttattgat ggattggata aattaaacca tcacaacgag
    10081 tggaaattat atgaaatttt tcataaa
//
LOCUS       DAZKAY010000092        10083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000092 DAZKAY010000000
VERSION     DAZKAY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.095"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACG8WP_002277"
     CDS             complement(109..2229)
                     /locus_tag="ACG8WP_002277"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9028554.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACG8WP_002278"
     CDS             2459..3637
                     /locus_tag="ACG8WP_002278"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9028555.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8WP_002279"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8WP_002279"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9028556.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8WP_002280"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8WP_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9028557.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACG8WP_002281"
     CDS             complement(4990..6243)
                     /locus_tag="ACG8WP_002281"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9028558.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8WP_002282"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8WP_002282"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9028559.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACG8WP_002283"
     CDS             7821..9167
                     /locus_tag="ACG8WP_002283"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9028560.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACG8WP_002284"
     CDS             complement(9475..9861)
                     /locus_tag="ACG8WP_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9028561.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="ACG8WP_002285"
     CDS             complement(9910..>10083)
                     /locus_tag="ACG8WP_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9028562.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DAZKAY010000093         9869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000093 DAZKAY010000000
VERSION     DAZKAY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.096"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACG8WP_002286"
     CDS             complement(18..263)
                     /locus_tag="ACG8WP_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9028563.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="ACG8WP_002287"
     CDS             complement(377..955)
                     /locus_tag="ACG8WP_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9028564.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="ACG8WP_002288"
     CDS             complement(1032..1562)
                     /locus_tag="ACG8WP_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9028565.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG8WP_002289"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG8WP_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9028566.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="ACG8WP_002290"
     CDS             complement(2419..2814)
                     /locus_tag="ACG8WP_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9028567.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACG8WP_002291"
     CDS             complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACG8WP_002291"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9028568.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACG8WP_002292"
     CDS             complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACG8WP_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9028569.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4842..5081)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACG8WP_002293"
     CDS             complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACG8WP_002293"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9028570.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="ACG8WP_002294"
     CDS             complement(6708..7865)
                     /locus_tag="ACG8WP_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9028571.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACG8WP_002295"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACG8WP_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9028572.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="ACG8WP_002296"
     CDS             complement(9332..9859)
                     /locus_tag="ACG8WP_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9028573.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcct
//
LOCUS       DAZKAY010000094         9834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000094 DAZKAY010000000
VERSION     DAZKAY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.097"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1346)
                     /locus_tag="ACG8WP_002297"
     CDS             complement(204..1346)
                     /locus_tag="ACG8WP_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028574.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1351..1536)
                     /locus_tag="ACG8WP_002298"
     CDS             complement(1351..1536)
                     /locus_tag="ACG8WP_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9028575.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1890..2135)
                     /locus_tag="ACG8WP_002299"
     CDS             complement(1890..2135)
                     /locus_tag="ACG8WP_002299"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028576.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2129..2530)
                     /locus_tag="ACG8WP_002300"
     CDS             complement(2129..2530)
                     /locus_tag="ACG8WP_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9028577.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="ACG8WP_002301"
     CDS             complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="ACG8WP_002301"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9028578.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4548..6314)
                     /locus_tag="ACG8WP_002302"
     CDS             complement(4548..6314)
                     /locus_tag="ACG8WP_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9028579.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6327..7121)
                     /locus_tag="ACG8WP_002303"
     CDS             complement(6327..7121)
                     /locus_tag="ACG8WP_002303"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9028580.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(7131..8507)
                     /locus_tag="ACG8WP_002304"
     CDS             complement(7131..8507)
                     /locus_tag="ACG8WP_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9028581.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(8627..9274)
                     /locus_tag="ACG8WP_002305"
     CDS             complement(8627..9274)
                     /locus_tag="ACG8WP_002305"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFP9028582.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(9358..>9834)
                     /locus_tag="ACG8WP_002306"
     CDS             complement(9358..>9834)
                     /locus_tag="ACG8WP_002306"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9028583.1"
                     /translation="GGDSMGDQASLTQNVVALFDTAEKSGCLPKTILYSLNPTDWLPL
                     ATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTTMAQQSLLSNFTGMLTDSRSFLS
                     YPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT         3157 a         1943 c         1238 g         3496 t
ORIGIN      
        1 aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa catggtttat tttttacact
       61 gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc gataccgaca
      121 gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta tgacagcagc
      181 gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt tgcgagtgct
      241 tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat agaggcatga
      301 cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa ggcaacatgc
      361 gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc tagttttttt
      421 attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa aaccaattct
      481 atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc aacggtttgt
      541 ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa ttcataatct
      601 tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat taagtcaaag
      661 tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa aataaaacgc
      721 atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt atatactttc
      781 ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat tttacctata
      841 gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa aactactctc
      901 aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat gccctttttt
      961 acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg tcgaatcttt
     1021 acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc tgttttcata
     1081 tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg ataaaaatca
     1141 tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg ttttaactct
     1201 tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac ttgaattctt
     1261 tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct ttttctgtct
     1321 ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat ggagaacttc
     1381 ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa agtatccttt
     1441 ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact gagaaaaacc
     1501 tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct tacttttttt
     1561 catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct ctccccaagt
     1621 ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct ttttattgac
     1681 tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat catggcgcaa
     1741 ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt ttcaatgaac
     1801 aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga ttcttttaaa
     1861 cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa gatcgcttct
     1921 attaatttaa tttctagctg acttatcata tattcatcta cataggtaac gaattgacct
     1981 acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata atgtttgagt
     2041 atcatcttga tcgaaattgg atctccttct agagcagata agataatgga tagtggaact
     2101 aataggtact gccgcttttt agatttatct accataggtc ataccccatt ttcaattgtt
     2161 caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga attttaccga
     2221 tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt cttatctccg
     2281 ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc attccaaata
     2341 agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca tttgaatctg
     2401 ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac ttagcttcgt
     2461 ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg tcattgtaat
     2521 gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca atcagaaagc
     2581 cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc tttaaacaga
     2641 aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt ttttgaacat
     2701 tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac catcgtttct
     2761 gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct ttcttatttc
     2821 cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac acttgttcac
     2881 caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa gccacttgat
     2941 actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta tctgctccat
     3001 attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct agctcttttt
     3061 tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca taataacgat
     3121 ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt gattttagat
     3181 aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat tcaactaaag
     3241 gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca atagaccaca
     3301 ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc gttttatgag
     3361 aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca tctaataaag
     3421 tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact acaatacctt
     3481 ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt ctaaaagaat
     3541 tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca ttcaaaccac
     3601 gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa ggtttattat
     3661 taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct tctgtatagg
     3721 tatcgatcac tttattatca tcaattaaac taattttcat actatataaa taagcgtcta
     3781 gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct tcagcttcag
     3841 ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc cattctgtat
     3901 cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata gtaatatcac
     3961 ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct gcataattga
     4021 agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt actgtttcct
     4081 tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca actgtaattc
     4141 gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta aacccacctt
     4201 tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt gcagatccaa
     4261 aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct ttttcatacc
     4321 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagagg
     4381 gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt tcttcctgta
     4441 agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt acttgcctcg
     4501 tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta aataaatgta
     4561 tcctttaaac cacaataagc atctattgga cttactccct taaattcaga aagacccatt
     4621 agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt aatataagtc
     4681 tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa aattgttgga
     4741 atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca gacaattcgt
     4801 tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt tttagttaaa
     4861 tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt ttcttcttgc
     4921 gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa tccttttgct
     4981 gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac tacttcacca
     5041 aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt ttctggtaca
     5101 ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc cacctcttta
     5161 aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt aggtaataaa
     5221 atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa aattcttttt
     5281 aaggcatcta ataaccgatc atcagtgata attcctttac gatagccttc catcatataa
     5341 ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc tgcaatagaa
     5401 gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg actagcatca
     5461 gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat taattctttt
     5521 gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg ttttgaataa
     5581 gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag
     5641 tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat actaaaagat
     5701 agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt
     5761 ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt atcagggttt
     5821 tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat atctacaata
     5881 ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac tctccctaat
     5941 tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc aacttcagta
     6001 ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa tagaggaatt
     6061 ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt tcctggatta
     6121 tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt tttatcacga
     6181 ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat agaatcaatc
     6241 gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt tcgtaaatct
     6301 acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca aaaagtatct
     6361 atatattttt gttcaatatc tacataatct cccgataaac atttaaataa aacatcagtt
     6421 cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa gaaaacttta
     6481 tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat cttatccaaa
     6541 tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt atcttgacaa
     6601 cacagttcat caactaatga taataattca tgtctttccc attcttcttg aacagcttcc
     6661 ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt tatagtatct
     6721 ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg attgacttct
     6781 tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat ttccctcttc
     6841 aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg attaatcgat
     6901 gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat tccacgagtc
     6961 tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg atgaacatca
     7021 aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat cgtatccata
     7081 acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc ctatttatgt
     7141 ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg ataaaggaaa
     7201 aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga agcgttatat
     7261 aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc tataatagaa
     7321 agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc aaaagaatat
     7381 accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc atcaataaca
     7441 tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata gccaaccgaa
     7501 ctaagaatta agaatacata catattacta gcttttatca aaaataagat caaatatact
     7561 attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt tttacttaaa
     7621 aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa attcattaaa
     7681 actattccct gtgcatcatt atagtaatca ggaaatacat aattattcat actcataatt
     7741 aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa tgccctattt
     7801 ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg gtcaattctt
     7861 actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca tatcgaaaat
     7921 attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc tgaatcatct
     7981 cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa agtagctcca
     8041 acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat agctgatgcc
     8101 attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa tatatacgta
     8161 acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt ttgatacatt
     8221 aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt aaacttaccc
     8281 tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt aactgcatcg
     8341 ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc caatactttt
     8401 gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt tccaaaatct
     8461 ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca attctctccc
     8521 ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga agcgtaaaga
     8581 gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa taattcttta
     8641 tctcataaaa cttatctata tatttactag ctactcgact tacttcttca aaatcacctt
     8701 tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct acttcaaacc
     8761 aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg tgtggtaatg
     8821 gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat agtttaatga
     8881 tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat
     8941 aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat ggactaacga
     9001 tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa accgtaccgg
     9061 ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg attatttctt
     9121 cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca ataattgaat
     9181 ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg actccagccg
     9241 cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt tatggataca
     9301 tactttgtct agctgtacta acttacaaag aataaattta agcttttatt tcaagtatta
     9361 ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt
     9421 ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt taacccactc
     9481 gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc ttagaaaact
     9541 tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt
     9601 caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat
     9661 tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcagt
     9721 tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag cggtatcgaa
     9781 caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac cacc
//
LOCUS       DAZKAY010000095         9705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000095 DAZKAY010000000
VERSION     DAZKAY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.098"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..2524
                     /gene="leuS"
                     /locus_tag="ACG8WP_002307"
     CDS             110..2524
                     /gene="leuS"
                     /locus_tag="ACG8WP_002307"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9028584.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2872..4425
                     /locus_tag="ACG8WP_002308"
     CDS             2872..4425
                     /locus_tag="ACG8WP_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9028585.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4806..6590)
                     /locus_tag="ACG8WP_002309"
     CDS             complement(4806..6590)
                     /locus_tag="ACG8WP_002309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028586.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6605..8326)
                     /locus_tag="ACG8WP_002310"
     CDS             complement(6605..8326)
                     /locus_tag="ACG8WP_002310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028587.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8426..9187
                     /locus_tag="ACG8WP_002311"
     CDS             8426..9187
                     /locus_tag="ACG8WP_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9028588.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3157 a         1719 c         1942 g         2887 t
ORIGIN      
        1 aagtttgaga aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt
       61 tatatttata atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa
      121 tcataaagaa attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac
      181 ccatgatgat cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg
      241 tcaagggcta catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt
      301 gaaaagaagt caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc
      361 tgctgaacaa tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat
      421 cgaaacattc cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt
      481 gaatacaact gatccggagt attataaatg gacacaatgg atctttacga agctttatga
      541 aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt
      601 tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag
      661 aaaaccaatg cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga
      721 tctagaatta gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg
      781 ttcagtagga gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt
      841 tacgacacga ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga
      901 tcttgttaga gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga
      961 gacagctaaa aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt
     1021 tactggtgct tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga
     1081 ttatgtatta tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg
     1141 tgactttgaa ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga
     1201 tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg
     1261 catgaacaaa caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg
     1321 gaaaaaagaa gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg
     1381 tgaaccaatc ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga
     1441 gctaccatta cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc
     1501 attagcaaat atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg
     1561 tcgcgaaacg aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat
     1621 cgaccctcat aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt
     1681 tgacatctac attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg
     1741 gcataaattc ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata
     1801 caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt
     1861 agtcaatcct gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat
     1921 gttcatgggg ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg
     1981 taaattcttg gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg
     2041 tatcactaca ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt
     2101 gacagaagac tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat
     2161 caatgaagcc tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact
     2221 actcgcaccg attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg
     2281 tggtatttct tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt
     2341 agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt
     2401 aggaaaagat gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga
     2461 aggcaaaaca gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa
     2521 ttaattggct ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa
     2581 tgaacttcat tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa
     2641 aggttttgat tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt
     2701 aagagatctc tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa
     2761 actacctgaa attgagagta cataccaaat tcataaattt tttattagtg ggactagtga
     2821 ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa
     2881 gaactattta ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa
     2941 agagcgaaaa gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt
     3001 ttcggagctg tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg
     3061 atgcaaaaat taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt
     3121 gtgcttattg tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc
     3181 gatcaatggc ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat
     3241 gatgactatc tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt
     3301 gattgccagt tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa
     3361 tcaaaattcg gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa
     3421 aaaagccagt cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt
     3481 tttttcctca ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg
     3541 tcttctcata taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata
     3601 caaaagacaa atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa
     3661 aagataaatg atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt
     3721 ggtgctaatt gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc
     3781 ctgacaacgg ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc
     3841 gatttctcat ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat
     3901 aatggtaaaa catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga
     3961 aaagcggtat tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct
     4021 gcagaaactt tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga
     4081 agtacaatga tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt
     4141 gtttcgacac gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca
     4201 gttctgtcga ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat
     4261 gaagttactc aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga
     4321 ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca
     4381 tggcaattcg ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg
     4441 agcctgacgt tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg
     4501 ttgaatagtg taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta
     4561 tagcttctcg tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg
     4621 tttattcgta aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa
     4681 ggagctatcc aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta
     4741 catttaaaaa agctatcgat catggactaa tcatacatga tcgatagctt tttagataat
     4801 gtttgttacg ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg
     4861 agtagttcag aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg
     4921 tctgcatttt caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc
     4981 acagcttcca ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct
     5041 tcatctaaaa taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt
     5101 tgctgtcctt gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc
     5161 agtgttcgaa taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt
     5221 gtggcatcca ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta
     5281 ttttgtaaaa ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca
     5341 agaccgtcga attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact
     5401 aaagtcgttt ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact
     5461 gaaaaatcga cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg
     5521 aaagctattt ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca
     5581 tctgtttcat ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag
     5641 ttgatcacat aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa
     5701 gcttgtaaga agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca
     5761 cttatgatga aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata
     5821 aattgagcac gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct
     5881 gcatgagctt gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt
     5941 cgattattta aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc
     6001 gcaatcttag ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc
     6061 ttgctttcga tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa
     6121 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
     6181 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
     6241 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
     6301 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
     6361 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
     6421 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
     6481 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
     6541 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
     6601 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
     6661 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
     6721 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
     6781 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
     6841 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
     6901 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
     6961 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
     7021 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
     7081 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
     7141 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
     7201 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
     7261 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
     7321 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
     7381 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
     7441 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
     7501 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
     7561 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
     7621 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
     7681 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
     7741 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
     7801 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
     7861 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
     7921 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
     7981 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
     8041 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
     8101 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
     8161 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
     8221 ctaattttgg tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga
     8281 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
     8341 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
     8401 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
     8461 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
     8521 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
     8581 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
     8641 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
     8701 actggtggtg atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt
     8761 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
     8821 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
     8881 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
     8941 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
     9001 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
     9061 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt
     9121 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
     9181 aaaataggaa atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa
     9241 aaattttctg gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa
     9301 ttttagtttt ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata
     9361 tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt
     9421 ataattcatg gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt
     9481 gtatcagatg agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt
     9541 gttgcttaca ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac
     9601 atatttttta atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga
     9661 tagcattttt ttgcttataa aataattata agtatttaga gattt
//
LOCUS       DAZKAY010000096         9666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000096 DAZKAY010000000
VERSION     DAZKAY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.099"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG8WP_002312"
     CDS             complement(<1..632)
                     /locus_tag="ACG8WP_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9028589.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG8WP_002313"
     CDS             975..1391
                     /locus_tag="ACG8WP_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9028590.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG8WP_002314"
     CDS             1388..1858
                     /locus_tag="ACG8WP_002314"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9028591.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG8WP_002315"
     CDS             1872..2645
                     /locus_tag="ACG8WP_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9028592.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG8WP_002316"
     CDS             2659..3471
                     /locus_tag="ACG8WP_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9028593.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG8WP_002317"
     CDS             3525..4490
                     /locus_tag="ACG8WP_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9028594.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG8WP_002318"
     CDS             4606..7329
                     /locus_tag="ACG8WP_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9028595.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG8WP_002319"
     CDS             7342..7974
                     /locus_tag="ACG8WP_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9028596.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9666
                     /locus_tag="ACG8WP_002320"
     CDS             8873..>9666
                     /locus_tag="ACG8WP_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028597.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattt
//
LOCUS       DAZKAY010000097         9609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000097 DAZKAY010000000
VERSION     DAZKAY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.100"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG8WP_002321"
     CDS             complement(25..162)
                     /locus_tag="ACG8WP_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9028598.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8WP_002322"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8WP_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9028599.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG8WP_002323"
     CDS             complement(1599..1853)
                     /locus_tag="ACG8WP_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028600.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG8WP_002324"
     CDS             complement(1878..3020)
                     /locus_tag="ACG8WP_002324"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9028601.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG8WP_002325"
     CDS             complement(3080..4432)
                     /locus_tag="ACG8WP_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9028602.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG8WP_002326"
     CDS             complement(4503..4847)
                     /locus_tag="ACG8WP_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9028603.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG8WP_002327"
     CDS             complement(4857..5231)
                     /locus_tag="ACG8WP_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9028604.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG8WP_002328"
     CDS             complement(5244..5558)
                     /locus_tag="ACG8WP_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9028605.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="ACG8WP_002329"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="ACG8WP_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9028606.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..>9609)
                     /locus_tag="ACG8WP_002330"
     CDS             complement(8978..>9609)
                     /locus_tag="ACG8WP_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028607.1"
                     /translation="NQSELKLILTINEFIEKHGITSSRQYQKKLSENPNSAPSMWFIN
                     KKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQRMYEQKSVGKN
                     IPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQDDLSMTKFRKLV
                     QSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKKTK"
BASE COUNT         2566 a         2194 c         1643 g         3206 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcgac atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atctaaggtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgatttt
//
LOCUS       DAZKAY010000098         9446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000098 DAZKAY010000000
VERSION     DAZKAY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.101"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..844)
                     /locus_tag="ACG8WP_002331"
     CDS             complement(176..844)
                     /locus_tag="ACG8WP_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9028608.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            978..1265
                     /locus_tag="ACG8WP_002332"
     CDS             978..1265
                     /locus_tag="ACG8WP_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028609.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1681..3219
                     /locus_tag="ACG8WP_002333"
     CDS             1681..3219
                     /locus_tag="ACG8WP_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9028610.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3216..4181
                     /locus_tag="ACG8WP_002334"
     CDS             3216..4181
                     /locus_tag="ACG8WP_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028611.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4168..5490
                     /locus_tag="ACG8WP_002335"
     CDS             4168..5490
                     /locus_tag="ACG8WP_002335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9028612.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5553..5657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5731..6474
                     /locus_tag="ACG8WP_002336"
     CDS             5731..6474
                     /locus_tag="ACG8WP_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028613.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6489..7482
                     /locus_tag="ACG8WP_002337"
                     /pseudo
     CDS             6489..7482
                     /locus_tag="ACG8WP_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            7494..8231
                     /locus_tag="ACG8WP_002338"
     CDS             7494..8231
                     /locus_tag="ACG8WP_002338"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028614.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8221..8943
                     /locus_tag="ACG8WP_002339"
     CDS             8221..8943
                     /locus_tag="ACG8WP_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9028615.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9027..>9446)
                     /locus_tag="ACG8WP_002340"
     CDS             complement(9027..>9446)
                     /locus_tag="ACG8WP_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9028616.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3108 a         1618 c         1981 g         2739 t
ORIGIN      
        1 gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg
       61 cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga ataatatcat
      121 tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt
      181 gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca
      241 attggggcaa aacagatcca atttccagat acttcacttt attcaatgta taagatagaa
      301 tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa
      361 ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt
      421 caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac
      481 cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag
      541 caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga
      601 cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta
      661 aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag
      721 caataccagt aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta
      781 atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt
      841 tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat actgacttat
      901 actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt tttatttatc
      961 aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca
     1021 aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa
     1081 tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac
     1141 ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc
     1201 agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga
     1261 tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt
     1321 ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc
     1381 tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt
     1441 gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta
     1501 gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca
     1561 gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa
     1621 gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc
     1681 atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt
     1741 caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc tattccttta
     1801 gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg
     1861 ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga
     1921 actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg
     1981 tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg
     2041 tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca
     2101 ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga
     2161 gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca
     2221 ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg
     2281 ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt
     2341 ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga
     2401 aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac
     2461 acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca
     2521 ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg
     2581 gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa
     2641 gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa
     2701 aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt
     2761 tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat
     2821 cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa
     2881 agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat
     2941 gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga
     3001 gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat
     3061 cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga tcaaatgatt
     3121 gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc
     3181 ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac
     3241 atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa
     3301 aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa
     3361 tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa
     3421 atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt
     3481 tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg
     3541 aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg
     3601 atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga
     3661 tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat
     3721 tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg
     3781 gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta
     3841 ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga
     3901 atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc
     3961 gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc
     4021 aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa
     4081 gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg
     4141 cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca
     4201 ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg
     4261 gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc
     4321 ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac
     4381 gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca
     4441 gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc
     4501 gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac
     4561 ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg
     4621 aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt
     4681 ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc
     4741 attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc
     4801 gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc
     4861 atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg
     4921 attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat
     4981 atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa
     5041 atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact
     5101 tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat
     5161 ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa
     5221 agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat
     5281 catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg
     5341 attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc
     5401 atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg
     5461 gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt
     5521 tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga
     5581 catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga
     5641 gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt
     5701 tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt
     5761 atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt
     5821 attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt
     5881 tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt
     5941 tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg
     6001 ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg
     6061 tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc
     6121 gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa
     6181 acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc
     6241 ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga
     6301 gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac
     6361 cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat
     6421 ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag
     6481 gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg
     6541 gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa agtgacactt
     6601 gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc
     6661 tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt
     6721 attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact
     6781 tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat
     6841 aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa
     6901 ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg
     6961 gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca
     7021 gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa
     7081 gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga tgaacggtta
     7141 gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg
     7201 gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat
     7261 gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa
     7321 tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa
     7381 gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat
     7441 gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa
     7501 tccaaatcag ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt
     7561 tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa
     7621 cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg
     7681 atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac
     7741 ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa
     7801 tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt
     7861 ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca
     7921 tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca
     7981 gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac
     8041 ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg
     8101 cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat
     8161 ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag
     8221 atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag
     8281 gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga
     8341 cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat
     8401 agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata
     8461 gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga
     8521 cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa
     8581 aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct
     8641 tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat
     8701 ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt
     8761 attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa
     8821 gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca
     8881 gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa
     8941 taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag
     9001 taaactgttt gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc
     9061 gccgcttccc aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt
     9121 gcagcagagt agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt
     9181 ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag
     9241 cttgtgcttg ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca
     9301 acaggcgctg cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg
     9361 actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt
     9421 gggattgtta attgttgtcc tgagta
//
LOCUS       DAZKAY010000099         9443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000099 DAZKAY010000000
VERSION     DAZKAY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.102"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACG8WP_002341"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACG8WP_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACG8WP_002342"
     CDS             282..611
                     /locus_tag="ACG8WP_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9028617.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACG8WP_002343"
     CDS             601..888
                     /locus_tag="ACG8WP_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9028618.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACG8WP_002344"
     CDS             1174..2031
                     /locus_tag="ACG8WP_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9028619.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACG8WP_002345"
     CDS             2032..2580
                     /locus_tag="ACG8WP_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028620.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACG8WP_002346"
     CDS             2840..3301
                     /locus_tag="ACG8WP_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFP9028621.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACG8WP_002347"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACG8WP_002347"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACG8WP_002348"
     CDS             complement(3397..4467)
                     /locus_tag="ACG8WP_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9028622.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACG8WP_002349"
     CDS             5069..6913
                     /locus_tag="ACG8WP_002349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9028623.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACG8WP_002350"
     CDS             6969..8435
                     /locus_tag="ACG8WP_002350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9028624.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACG8WP_002351"
     CDS             8472..9314
                     /locus_tag="ACG8WP_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9028625.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1443 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggctcca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atc
//
LOCUS       DAZKAY010000100         9431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000100 DAZKAY010000000
VERSION     DAZKAY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.103"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG8WP_002352"
     CDS             <1..680
                     /locus_tag="ACG8WP_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9028626.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG8WP_002353"
     CDS             800..1000
                     /locus_tag="ACG8WP_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9028627.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG8WP_002354"
     CDS             1076..1273
                     /locus_tag="ACG8WP_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028628.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG8WP_002355"
     CDS             complement(1570..3117)
                     /locus_tag="ACG8WP_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9028629.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG8WP_002356"
     CDS             complement(3201..4004)
                     /locus_tag="ACG8WP_002356"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9028630.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8WP_002357"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8WP_002357"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9028631.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8WP_002358"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8WP_002358"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9028632.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8WP_002359"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8WP_002359"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9028633.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG8WP_002360"
     CDS             complement(6389..7318)
                     /locus_tag="ACG8WP_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9028634.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8195
                     /locus_tag="ACG8WP_002361"
                     /pseudo
     CDS             7513..8195
                     /locus_tag="ACG8WP_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            8356..9399
                     /locus_tag="ACG8WP_002362"
     CDS             8356..9399
                     /locus_tag="ACG8WP_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9028635.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2736 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttg
     7621 aaaaggaaga gcacctcatt caaacgggaa aaacctcaga ttatttgtat tttttagtgg
     7681 atgggacggt aatggtgtac tcgtattctt ctgatacaca aaatatttgc atcgattacg
     7741 caaagcccgc tacaccactc ggcgaagcct cttcattgtg ggaattatcg ccgaaaagca
     7801 gcgttaaagc agtgactaag tgcatctgta tcagcattcc gctaaatcaa tatcgaacac
     7861 agcttcaaaa agatgttcgc tttttgcaga atatttgcca gttactaagt tatcgtttaa
     7921 attccgggat aaatttggcg aactcactga cagaacctgt tgaaactcgt ttagcaaagt
     7981 ttattttgac ccatcacgaa aacgaacgtt tctcatttcg gttaaccacc tgcgcctcaa
     8041 tcttaaatgt cagttatcga catcttttac gaacaatcac gaattttcgt gaggagaata
     8101 ttatcgaaaa gaaaaagaat tattacttga ttcatgatat cgaagcttta gaggacttag
     8161 ctgagaatat aagtagtcca gtaaaagcag aataacagct ataaaaaaac agcagtcaga
     8221 agtgattgct ggttttttga tagtggagaa attgggaaac ggaaaatctt cgttgaagag
     8281 tagtgttcta tatattggta tgctatactt ttaaaagttc aatgtgtcaa gacacataaa
     8341 aaggggagaa aaattttgaa gaaaatactt gtttcattgt tgtttttggt gtcattagta
     8401 acgttagtcg gctgtagtca gtctcagggg gagaagcaaa gtgaaaaaga gaaaagtaaa
     8461 atcgctttgg ttcttggatt tggcggggtt gatgaccgct ccttcaatca agctggctgg
     8521 gaaggattag aggcttgggg aaaagagaaa aagctgacag caaaaaaaga ttttacgtat
     8581 atagaaacga acaatccaga ggataacagt accaatatcg atcaggcaat cagaaacggc
     8641 tttaccacca ttattagctt gagctacttg caagcagagg aactggaaaa tgctgctaaa
     8701 gataatccaa agttgaaatt tgccttggta gatggtgaga ttactgggaa aaagaatatc
     8761 tcatcagcaa cctttaaaga tcaagaaagt gcgtatttag caggactagc tgccgcatat
     8821 acgacaaaaa gtgatcaagt aggctttatt ggtggaatgg aagggcccgt gatccaacgt
     8881 ttccaaagcg gatttgaagc tggcgttaag gcaggggcca cagcactgaa caaggaaatt
     8941 actgttaacg tacaatatgc tgcatcctat gattcgctag ataaaggaaa agctattgct
     9001 gcttctatgt acaaagaggg agtcgatatt ctttatcagg cggcaggaag tactggagct
     9061 ggggtgttcc aagaagcaaa agcccgcaat gaaaacgact atagcagaaa agtatgggtg
     9121 attggtgtag atcgcgatca agcggatgaa ggggattata aaatggagaa cggcgagaca
     9181 gggaatttca cgttgacttc tacaaagaaa aatgtcggtg cagaagtcaa agaatttgca
     9241 gaaacttctc ataacgataa atttcctggc ggcaagcatt tgacctttgg tttgaaagaa
     9301 gaaggggtag atctgacaaa aggacaatta tcagaggaag cctatcaggc agtttcagac
     9361 gcaaaagaga aaatcatcag tggtgatttc agactgtaga caaagtccat tttatggatg
     9421 atgtctacag t
//
LOCUS       DAZKAY010000101         9401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000101 DAZKAY010000000
VERSION     DAZKAY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.104"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8WP_002363"
     CDS             168..362
                     /locus_tag="ACG8WP_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028636.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8WP_002364"
     CDS             631..930
                     /locus_tag="ACG8WP_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028637.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8WP_002365"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8WP_002365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028638.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8WP_002366"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8WP_002366"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9028639.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8WP_002367"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8WP_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9028640.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8WP_002368"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8WP_002368"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9028641.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8WP_002369"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8WP_002369"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9028642.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8WP_002370"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8WP_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9028643.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8WP_002371"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8WP_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9028644.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8WP_002372"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8WP_002372"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9028645.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8WP_002373"
     CDS             7521..8291
                     /locus_tag="ACG8WP_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9028646.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8WP_002374"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8WP_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028647.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2919 a         1924 c         1481 g         3077 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc c
//
LOCUS       DAZKAY010000102         8917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000102 DAZKAY010000000
VERSION     DAZKAY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.105"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..597)
                     /locus_tag="ACG8WP_002375"
     CDS             complement(127..597)
                     /locus_tag="ACG8WP_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028648.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            684..1382
                     /locus_tag="ACG8WP_002376"
     CDS             684..1382
                     /locus_tag="ACG8WP_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9028649.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1560..1901
                     /locus_tag="ACG8WP_002377"
     CDS             1560..1901
                     /locus_tag="ACG8WP_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028650.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1894..2565
                     /locus_tag="ACG8WP_002378"
     CDS             1894..2565
                     /locus_tag="ACG8WP_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9028651.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2571..3164
                     /locus_tag="ACG8WP_002379"
                     /pseudo
     CDS             2571..3164
                     /locus_tag="ACG8WP_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
     gene            3256..3786
                     /locus_tag="ACG8WP_002380"
     CDS             3256..3786
                     /locus_tag="ACG8WP_002380"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFP9028652.1"
                     /translation="MNELWMDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIM
                     KPSDNGKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVEN
                     LEWLSSDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRH
                     TVLNIASGKTFSFLED"
     gene            3891..4721
                     /locus_tag="ACG8WP_002381"
     CDS             3891..4721
                     /locus_tag="ACG8WP_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP9028653.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            4738..5589
                     /locus_tag="ACG8WP_002382"
     CDS             4738..5589
                     /locus_tag="ACG8WP_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028654.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            5586..5945
                     /locus_tag="ACG8WP_002383"
     CDS             5586..5945
                     /locus_tag="ACG8WP_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFP9028655.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            5960..6121
                     /locus_tag="ACG8WP_002384"
     CDS             5960..6121
                     /locus_tag="ACG8WP_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9028656.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            6118..6423
                     /locus_tag="ACG8WP_002385"
     CDS             6118..6423
                     /locus_tag="ACG8WP_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028657.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            6423..6740
                     /locus_tag="ACG8WP_002386"
     CDS             6423..6740
                     /locus_tag="ACG8WP_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9028658.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            6737..7132
                     /locus_tag="ACG8WP_002387"
     CDS             6737..7132
                     /locus_tag="ACG8WP_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9028659.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            7129..7467
                     /locus_tag="ACG8WP_002388"
     CDS             7129..7467
                     /locus_tag="ACG8WP_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028660.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            7464..7832
                     /locus_tag="ACG8WP_002389"
     CDS             7464..7832
                     /locus_tag="ACG8WP_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028661.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            7829..8134
                     /locus_tag="ACG8WP_002390"
     CDS             7829..8134
                     /locus_tag="ACG8WP_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028662.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            8158..8361
                     /locus_tag="ACG8WP_002391"
     CDS             8158..8361
                     /locus_tag="ACG8WP_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9028663.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            8358..8654
                     /locus_tag="ACG8WP_002392"
     CDS             8358..8654
                     /locus_tag="ACG8WP_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028664.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            8730..>8917
                     /locus_tag="ACG8WP_002393"
     CDS             8730..>8917
                     /locus_tag="ACG8WP_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9028665.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQM"
BASE COUNT         3330 a         1332 c         1930 g         2325 t
ORIGIN      
        1 gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa
       61 aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac
      121 tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc
      181 aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag
      241 gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt
      301 aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc
      361 tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga
      421 aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt
      481 ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat
      541 tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca
      601 ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta
      661 taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
      721 tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag
      781 atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg
      841 aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct
      901 tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag
      961 cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata
     1021 tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag
     1081 atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag
     1141 ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat
     1201 tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac
     1261 ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga
     1321 tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat
     1381 agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa
     1441 ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag
     1501 tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata
     1561 tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga
     1621 aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag
     1681 cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc
     1741 atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg
     1801 atgcgctaga agagtacgac agagataact tgattgatat ctcttattac ggaccaggta
     1861 agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac
     1921 tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat
     1981 tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta
     2041 actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt
     2101 gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg
     2161 gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt
     2221 gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg
     2281 gctctacacg gactgggatt atatgtattt caaggggaag atattccaac accacctaga
     2341 atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg
     2401 ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg
     2461 ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg
     2521 gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac
     2581 cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa
     2641 ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg
     2701 atcgagtcat tgaaattcgt tttattgatc ctagacgttt cactgtacag caacgaaact
     2761 tcatctatgc gctgataggc gatattttca tcgatacagg catgccaacg gacttctgga
     2821 aggaattctt ctacttccgt ttcgaaggtg tcacagggcg caaaataagc ctcaaagatg
     2881 aatcgagcac aaccgtaagt gatgccaata tcttagcgaa tatcatccta gatttcatct
     2941 ttgagcatga tattccgttt aaaaatggat atgaaatttt gccccaaaac gaacagtatt
     3001 tcttttacaa atgcctcacg aacagggtgt gctgtagttg cggaaagaaa aatgctgata
     3061 tacatcacgt agactctgtc ggcatgggaa ataaccgaaa aaaaataaac aattctggta
     3121 ggcgctttat ggctctgtgc agagaatgcc atacgaaaat tcatgtcgaa ggttttacta
     3181 catttacaac taaaagaaag cttacggcag ttgttctcaa agacagtgat ttaaaaagat
     3241 tggggttgaa tatcattgaa tgaattatgg atggatatcg aaggatacga gggagtatat
     3301 caagttagta gcttaggaag agttaaatct aatcgcccta actatgtaat taataaggga
     3361 tacgttaaca aagaaggatt tattatgaaa ccatctgaca atgggaaagg gtatcaaatt
     3421 atcttcttaa gcggcaaagg ttttagagat agaaggtatg tccatcggct agtagctttt
     3481 cactttttaa aagaagcgta tttcgaaggt gctgaagtca atcataagaa cggagataaa
     3541 tctaacaata ctgttgaaaa cctagaatgg ctcagttctg atgataataa aaagcatgct
     3601 agaaaaaatg gattaactaa cttgaaagga ccatcaaaac taactgattt gcaagcttta
     3661 gctatcaagc gtttatatac aaacaaattg atgagctccg gagaaataat gaaacttttt
     3721 aatgtcagca ggcataccgt gctgaatata gcttcaggga agacatttag tttcttggaa
     3781 gattaagaga aacacaaaat aggtgtgact aatttcaaaa ataaatatca aatcaaaggg
     3841 atcaagttaa accaggaaac aattaaaaag ttaaggatag gaggataaat ttggctgaga
     3901 taagttggat caaacttaaa actactatgt ttgacgatga aaaaatacga ttaatccaag
     3961 ctgttcctga gtcggatgcc atcatcgtta tatggattcg attactagtt ttagcaggaa
     4021 agactaacga cgatggtctg atatatatcc agaggaacat gccttatacc gaagaaatgc
     4081 ttgctacatt gtttggcaaa aacgtaaata cggttcgctt agcgctaact acattggcaa
     4141 atttcaacat gattgatcta agcagtgatg gactaattgc catcagtaat tgggaaaaac
     4201 atcaaaatat cgagggtatg gataaagtaa ggctaaaaaa tgctgaaaga aaccgtaaat
     4261 acagggaaag aaagagacag gaacgtctca aattggaaaa tgacgttagc gtgacgtcac
     4321 gtgacggtac agataaagat atagaagaag ataaagatat agataaagaa gaaaagaaag
     4381 gtaagtattc tgacgaacac ttacgccttg ctaaaaagtt gcaaagtaat ttaactgaag
     4441 attttccaaa agaaatgaac aaagtagata tcgaaaaatg ggcagacaca atcaggttga
     4501 tggaagaaag agataaagca tctatagaag cgattgagta tgtgatcaat tggctaccta
     4561 caaatgaatt ttggtttgga aatattagaa gtgctaagaa attgagagaa aaatttgaga
     4621 agctcaaatt cgaaatcaaa gcagacaaga agaatcataa aaagcaaagt caaaaactac
     4681 agtacagcaa tcctagtgaa tatgacgact tgccaattta aaaaggagat gcatcacatg
     4741 gaaagcctag caaatgctat ggagaaacta ataagaagag tattagtgca aagcggaaaa
     4801 tgtccagaat gtagcgaacc tttgtatagt tggcgagcta aaaataagga tggttcagaa
     4861 cgttgtaaac caacatgcat gagttgtggt tataaagcgt tacgtgtgaa agaggatata
     4921 cagaccgaac ggatatataa cgatagctta aaagcacgag cgttgagttt ttttcaaaat
     4981 ggttcggtat taacagataa aactttgttt aaatgcaaaa tggaaaatta tcacgtagtg
     5041 gatcaagaaa caaaaatcgc tttagaaaaa gcaaaaagct atacgaatga ggttctgctg
     5101 aaccatcctg cacatttcat tctatcaggg aaatcaggaa gcggaaaaag ccacttatca
     5161 atggccacag cttgggaaat acttgagcgc tcaaattatg acaagaaaat actttttata
     5221 agctatcaag aattattaga gcaaataaag ttttcttata acaatactga actgagaaaa
     5281 gaaattgaag gatcgcttat agccgatatt aaaacaactg atttggtggt ttttgacgat
     5341 attggagctg aattaggtag tggggtatca aatagtaggc agtttacaaa caacacgtta
     5401 aacacgctct tagaagccag acagaacaag gcaacgatca tcacaacaaa cttatctggt
     5461 cctgaactaa gagaagccta cggtgaaaga attgtttcta ggatatttaa gaattcagaa
     5521 ggttatgcgc tgaaattcca acaaacagca gacaagcgca taaaaccagt gaaaggtagt
     5581 atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag tttgattcta
     5641 tcgcagaagc agagtattac gatctagcct tgtggcaagc tgaagcaaat ggctggaaag
     5701 taaaacttca ggaaagattt gagctgatgc cgaaatttga actagacgga aagaagtatc
     5761 gcaagatcga gtatattccc gacttcacat tttataaaaa cggcaaactt gtcaaagtcg
     5821 tagatgtcaa aggaatgcag acaaaagact ttaagatcaa ggcaaagttg ttctgtcatc
     5881 aatatcaagc gccgttgata ttagctaaaa aatatcggaa tacgttcaag gaagagcgtt
     5941 tttaacgagg tggtccatca tgacaacaga agaagtgatt caaatgcgta ttcgaaacat
     6001 tcagcgtgaa attgacgatc tagaacgaac aaaggcagtg atggtcaatg aaacggcgag
     6061 aaaggcaatc gatttgcaca tagagaactt aagaagggaa attcgtagat tggaggaatg
     6121 agcgtggata agaaagcgac aatgaaacga attgctgaat taaccaagtc agaatcttgg
     6181 caggaagaca aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa
     6241 aagcctaaac ggaaaacgcc gagaaaaatt gcaatctggc atggtgatcg aattctagta
     6301 acaggtacag ctgaacagtt atctgaaatt actggattaa gcaaaaacat tatctgggat
     6361 agagctagga gcttatggat tgattcaaaa ggacgacagt ttaggtatgt ggaggagaga
     6421 taatggatct cattacacaa tacagtgaca tcatcctcaa gaaaatcatg atgaagattc
     6481 agaaagacaa aaaatcaaaa gaacgagcgg aattagttaa gttggaaatg gctgaaacag
     6541 gagcaggagt gcgaagtagc aggcattgga aagcagcagc aaacattgaa ttttattaca
     6601 acgaaattca aaaagggttc gatcagatgc gtgagctgga tcggcaaaca aattggagca
     6661 agaaacttca tcaagatcgt ttcaagtttg taaagaaata taaagagata ttagacaaat
     6721 acatggagga cagcaaatga tactgaagta cagggcatgg aataagaaaa ctcagtcatt
     6781 tattgattat ggtgatttgg ttttagattt gagaagcgga aaaatttacg ctggggacat
     6841 tgggttagtg gaaagcacca ttgatgtgac tgaccaaatt gaactcatgc aatccacagg
     6901 aatgaaagat aagaatggtg tggagatatt tgaaggggat gtagtaatta catcaaaagg
     6961 agcgattgga tatgttgcgt atcttcaaca agaagccggc tttgtagttg tactaaagaa
     7021 aagtgattat agattaggcc ataggaatac tggcgaaagt tatgatgtag caactgctca
     7081 tgaagtcatc ggaaatatat acgagaatag cgagttattg gaggtagagt gattatctta
     7141 gctactgact acatcgattc tttaaaagat gaaaatggga atgtcccagc attgctaaat
     7201 tgtggaatta aaagcaagaa tagaatcatt ttgttacgga tatcggctga tttagcaaaa
     7261 tatctttatc tgagcaagac agcagtatat tacactgcgg ctacacgtta tcagtacaag
     7321 ggaaaaacaa tcagtcagga ttattatgat cgtttaatca gtcctgacat gcatggaaaa
     7381 tcaaaatcag cgatcaagtt atttggcgca atagagatat atgccgatga ttttccaaat
     7441 ttatggttga aggaggaagc ggaatgaacg aggaaataaa aaaatctatt cgttacattg
     7501 atagagcgtt atcgcaactc gaacccttgc gaattgaaag tcataaagga atgagtgtct
     7561 acccaaatca gtatgacaaa atgaacgctg atttacgtaa tgcaaagctg gccttaatta
     7621 acattaaaaa taacaaaggc tgccagtatt gcaaacacag cgaaccgttg aatgatacca
     7681 gcaactctaa ctttgcaact gcaattgagt gtgaggaaaa tggatactac attgcaactg
     7741 agtatgacga tggcgattat gcagataatg gctattctgc ttatatccaa tattgcccta
     7801 tgtgcggtcg aaaattggag gtagcggaat gaatgttcaa gtttatttaa aaagaaattt
     7861 ggccaggttt cctcgaagct gggatacaaa ttcccaccca tccgaggcag aagaagagag
     7921 ctatctaatg tctgctaaca tgatagaaat tacaagagat gatgcggatg agtttgataa
     7981 aaaaagatca tggtactcgg tttcgaatcc aatgtatacg gctgtaatag aagattatca
     8041 tatatccgac agatttattc tggttgatct agaaagacct aagaaacgaa ttaaacagta
     8101 tagacgttgc gggttttcaa caaagaaatg gtgaaaacaa aacagaggtg actcagaatg
     8161 aacaacagac atcgcagaat aacaaaacta agaaaacagg aactgaatgt actaaagaca
     8221 aagtttgaaa aagaatacgg agtttctgca gaagaaacat ataaagtggc aagtcagtgt
     8281 gttgctgatg caagtgatgc tattcgtaag tttggaattt cgatattaag tgatgatcgt
     8341 aaatgggagg aaaaagaatg aaactaaaag acggatttta cgctagtagc cacggtatcg
     8401 gcggtttaat gctggatatg cagacaaaga accctaaaac acgtaagaaa ccaaaattca
     8461 aagtcggtga catggttcgc tgcgaagcag aagggttcat ctatccattt cgtggatatg
     8521 tagaacatgt ctataatcac tcagcgatca ttcgcattga aaacacgatg gaatgcgaca
     8581 agttattagc gaaaagcaaa gagaatttag cagtggtgag attggtggat attgaactaa
     8641 tcaatgacaa ataaaaaagc cggatcgctc cgactaacat aataaaacag acaagtttat
     8701 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
     8761 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cgtatggcag
     8821 gtcgctcttt gatagatatt aagtcgccga ttataacgga tatgccgaag gcgccgaagc
     8881 acggcaataa ggcagaggac gcgatcattc agatgat
//
LOCUS       DAZKAY010000103         8808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000103 DAZKAY010000000
VERSION     DAZKAY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.106"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..698)
                     /locus_tag="ACG8WP_002394"
     CDS             complement(36..698)
                     /locus_tag="ACG8WP_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028666.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            882..2528
                     /locus_tag="ACG8WP_002395"
     CDS             882..2528
                     /locus_tag="ACG8WP_002395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9028667.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2519..3601
                     /gene="uxuA"
                     /locus_tag="ACG8WP_002396"
     CDS             2519..3601
                     /gene="uxuA"
                     /locus_tag="ACG8WP_002396"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9028668.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3709..5262)
                     /locus_tag="ACG8WP_002397"
                     /pseudo
     CDS             complement(3709..5262)
                     /locus_tag="ACG8WP_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5410..6551
                     /locus_tag="ACG8WP_002398"
                     /pseudo
     CDS             5410..6551
                     /locus_tag="ACG8WP_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(6677..7756)
                     /locus_tag="ACG8WP_002399"
     CDS             complement(6677..7756)
                     /locus_tag="ACG8WP_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9028669.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7859..8299)
                     /locus_tag="ACG8WP_002400"
     CDS             complement(7859..8299)
                     /locus_tag="ACG8WP_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9028670.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(8356..8766)
                     /locus_tag="ACG8WP_002401"
     CDS             complement(8356..8766)
                     /locus_tag="ACG8WP_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9028671.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         3008 a         1568 c         1614 g         2618 t
ORIGIN      
        1 ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa
       61 cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat
      121 cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg
      181 atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg
      241 gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct
      301 tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt
      361 tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa
      421 cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa
      481 acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc
      541 tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc
      601 agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga
      661 atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt
      721 ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag
      781 taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc ataaaattca
      841 cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact
      901 ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg
      961 catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc
     1021 tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca
     1081 ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt
     1141 ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat
     1201 tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga
     1261 tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag
     1321 gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa
     1381 atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt tataaacgtt
     1441 ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca caaaacggta
     1501 gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac
     1561 aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga
     1621 ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct
     1681 tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata
     1741 cagaggacat ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg
     1801 aaaaagctgg ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg
     1861 tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt
     1921 ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg
     1981 ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc
     2041 tggaagaagt aattactgtg cgcttttcta atccaaatat tcctaatatg cctcagcgaa
     2101 tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata
     2161 tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt
     2221 ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc
     2281 cactattgac agaccttcag cataagcttt ccgatattca actgggtacg caagatacat
     2341 cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc
     2401 tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac
     2461 caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat
     2521 ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag
     2581 acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt
     2641 atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact
     2701 aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga
     2761 gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt
     2821 catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt
     2881 agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga
     2941 aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat gggaacctga
     3001 gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact
     3061 tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt
     3121 caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag ctcgaatcgt
     3181 aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt ataacggaat
     3241 cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc
     3301 agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc
     3361 taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag acatgtaccg
     3421 catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg atcatggaag
     3481 aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc
     3541 tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta
     3601 gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata
     3661 aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac
     3721 ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc
     3781 tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata
     3841 gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca
     3901 gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt
     3961 aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg
     4021 cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt
     4081 ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa
     4141 ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa gcatgaaagt
     4201 aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc
     4261 gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca
     4321 aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag
     4381 tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa
     4441 aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac
     4501 agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt
     4561 cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat
     4621 cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc
     4681 cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata
     4741 aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt
     4801 attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct ttttcatttt
     4861 cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa tcgaatttgt
     4921 ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc
     4981 aaatagacga taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg
     5041 tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc
     5101 tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt
     5161 agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt
     5221 tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg ctccttttta
     5281 cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga caaaaaaaca
     5341 gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt
     5401 gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg
     5461 cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg gacagaaacc
     5521 aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga
     5581 gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcggga
     5641 gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc cactttgtcg
     5701 attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc
     5761 gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga
     5821 ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca
     5881 gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat
     5941 aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct
     6001 ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa acctgaaata
     6061 acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg aaaccaaaca
     6121 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
     6181 gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga
     6241 ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa
     6301 tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt
     6361 gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctgtcctg
     6421 aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga
     6481 gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca
     6541 gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc
     6601 agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc
     6661 tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt
     6721 tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa
     6781 cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg
     6841 ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa
     6901 tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata
     6961 gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga
     7021 tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg
     7081 tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta ttgacaaact
     7141 tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc
     7201 ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca
     7261 tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc
     7321 cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa
     7381 caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg atcatgtcaa
     7441 atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga
     7501 tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga
     7561 tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca
     7621 tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt
     7681 tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa
     7741 taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa aagaaaaacc
     7801 gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc
     7861 agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg
     7921 tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg
     7981 cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt ctttcatcac
     8041 ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca
     8101 gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt
     8161 cccataaaac taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg
     8221 gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat acttgattaa
     8281 ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga
     8341 tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt
     8401 agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag
     8461 agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc
     8521 tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt
     8581 gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg
     8641 gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc
     8701 gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt
     8761 tttcataaaa ttaccacctt taattatatt ttatacaatt gaatttta
//
LOCUS       DAZKAY010000104         8774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000104 DAZKAY010000000
VERSION     DAZKAY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.107"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..2753)
                     /locus_tag="ACG8WP_002402"
     CDS             complement(372..2753)
                     /locus_tag="ACG8WP_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9028672.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ACG8WP_002403"
     CDS             complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ACG8WP_002403"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9028673.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3454..3993
                     /locus_tag="ACG8WP_002404"
     CDS             3454..3993
                     /locus_tag="ACG8WP_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9028674.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4083..4502
                     /gene="gpsB"
                     /locus_tag="ACG8WP_002405"
     CDS             4083..4502
                     /gene="gpsB"
                     /locus_tag="ACG8WP_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9028675.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4524..4891
                     /gene="rnpB"
                     /locus_tag="ACG8WP_002406"
     ncRNA           4524..4891
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8WP_002406"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5100..6266
                     /locus_tag="ACG8WP_002407"
     CDS             5100..6266
                     /locus_tag="ACG8WP_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9028676.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6312..7808
                     /locus_tag="ACG8WP_002408"
     CDS             6312..7808
                     /locus_tag="ACG8WP_002408"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9028677.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7889..8572
                     /locus_tag="ACG8WP_002409"
     CDS             7889..8572
                     /locus_tag="ACG8WP_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9028678.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8581..>8774
                     /locus_tag="ACG8WP_002410"
     CDS             8581..>8774
                     /locus_tag="ACG8WP_002410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028679.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2772 a         1585 c         1850 g         2567 t
ORIGIN      
        1 acaaattatg ataattaagc atactatcag ataactcaaa atgaagcctt ctttcaaaaa
       61 attattcaaa accatatatt gtatttttta tttgataata acaatgggat tattaagaag
      121 tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca agtaaatgga
      181 taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct aaatgttaga
      241 tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga
      301 tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg
      361 tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt
      421 gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac
      481 ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt
      541 gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag
      601 ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa
      661 gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag
      721 tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg
      781 ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct
      841 gaagtgacag gcgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc
      901 gtatagcccg caaataggat atctggatac accccagaag aaataccaag ttttgcatac
      961 tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc
     1021 gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct
     1081 gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg
     1141 aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca
     1201 taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta
     1261 ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag
     1321 aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg
     1381 gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct
     1441 acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa
     1501 tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa
     1561 tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt
     1621 ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc
     1681 atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt
     1741 tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct
     1801 gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg
     1861 tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca
     1921 tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt
     1981 ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca
     2041 gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca
     2101 gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc
     2161 aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc
     2221 ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc
     2281 tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg
     2341 atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg
     2401 atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg
     2461 cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct
     2521 aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct
     2581 gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct
     2641 tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt
     2701 ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct
     2761 ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg
     2821 cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga
     2881 tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata
     2941 aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag
     3001 gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt
     3061 gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc
     3121 tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata
     3181 gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga
     3241 aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa
     3301 ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata
     3361 aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag
     3421 caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg
     3481 tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt
     3541 gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta
     3601 cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa
     3661 gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac
     3721 tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa
     3781 gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata
     3841 cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag
     3901 tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc
     3961 agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt
     4021 ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa
     4081 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
     4141 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
     4201 aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
     4261 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
     4321 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
     4381 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
     4441 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
     4501 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
     4561 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
     4621 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
     4681 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
     4741 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
     4801 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
     4861 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
     4921 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
     4981 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
     5041 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     5101 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     5161 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     5221 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     5281 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     5341 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     5401 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     5461 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     5521 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     5581 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     5641 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     5701 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     5761 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     5821 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     5881 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     5941 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     6001 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     6061 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     6121 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     6181 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     6241 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     6301 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     6361 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     6421 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     6481 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     6541 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     6601 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     6661 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     6721 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     6781 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     6841 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     6901 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     6961 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     7021 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     7081 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     7141 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     7201 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     7261 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     7321 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     7381 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     7441 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     7501 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     7561 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     7621 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     7681 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     7741 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     7801 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     7861 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     7921 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     7981 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     8041 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     8101 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     8161 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     8221 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     8281 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     8341 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     8401 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     8461 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     8521 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     8581 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     8641 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     8701 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     8761 gaacaccgtt tatt
//
LOCUS       DAZKAY010000105         8739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000105 DAZKAY010000000
VERSION     DAZKAY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.108"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1106
                     /locus_tag="ACG8WP_002411"
     CDS             99..1106
                     /locus_tag="ACG8WP_002411"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP9028680.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1118..2122
                     /gene="neuB"
                     /locus_tag="ACG8WP_002412"
     CDS             1118..2122
                     /gene="neuB"
                     /locus_tag="ACG8WP_002412"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFP9028681.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2119..3267
                     /gene="neuC"
                     /locus_tag="ACG8WP_002413"
     CDS             2119..3267
                     /gene="neuC"
                     /locus_tag="ACG8WP_002413"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP9028682.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3240..3917
                     /locus_tag="ACG8WP_002414"
     CDS             3240..3917
                     /locus_tag="ACG8WP_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9028683.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3868..5049
                     /locus_tag="ACG8WP_002415"
     CDS             3868..5049
                     /locus_tag="ACG8WP_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9028684.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5062..6039
                     /locus_tag="ACG8WP_002416"
     CDS             5062..6039
                     /locus_tag="ACG8WP_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9028685.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6039..6947
                     /locus_tag="ACG8WP_002417"
     CDS             6039..6947
                     /locus_tag="ACG8WP_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFP9028686.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            6948..7700
                     /locus_tag="ACG8WP_002418"
     CDS             6948..7700
                     /locus_tag="ACG8WP_002418"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9028687.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            7705..8652
                     /locus_tag="ACG8WP_002419"
     CDS             7705..8652
                     /locus_tag="ACG8WP_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9028688.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3215 a         1103 c         1674 g         2747 t
ORIGIN      
        1 acgttttgac atcattattg ataattagtt tagttatata ttttttaaag attaagaatc
       61 gaattataga tgactataat tcataggagg agatataagt gtttaaagga aaaacaatat
      121 tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg
      181 aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat atgcaacaag
      241 agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac tctcaaagga
      301 ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg aaacacgttc
      361 cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga actcaaaatg
      421 taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt acagataaag
      481 tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa ttaataatag
      541 ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg tttggaaatg
      601 tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa aacaataaag
      661 atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg gatgctgcaa
      721 gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc ttaaaaatgc
      781 cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat gatacacatg
      841 atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa aaattatatg
      901 aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa atgtatatca
      961 ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa attaaaatca
     1021 ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt agaatcgcaa
     1081 aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag tatatgtaat
     1141 tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa gtattgataa
     1201 agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga ataagctagt
     1261 ttccaaaaag gctccaatgg caaaatatca gaaggataat cttaaagagg acagttctca
     1321 atttgaaatg ctaaaaaaat tagaattatc tcaatctgat tttattagaa taaaagatta
     1381 ttgtatacaa aataaaattg attttttgtc tactcctttt gatgaagaaa gtgcagactt
     1441 tctaaaacaa attggtgtac aaggatttaa aattggctca ggagatttaa caaatttacc
     1501 ttttcttaga aaaatagata tgtatgggtt gccagtttta ttatctacag gtatggctga
     1561 tattcatgag gtcagtgagg ctgtagattg tttcatagat agtccagtta ctattttaca
     1621 ttgtacttca aattatccgg ctagttctga agatattaat ttattggcga ttaacacatt
     1681 aaaagaaatg ttcgggatac ccgttgggta ctccgatcat tcattgggat atgatgttgg
     1741 tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa cattttacct tggataagga
     1801 actacctggt ccagatcata aagcttcttt agatcctcag gaatttgcag attttgtaaa
     1861 gcatattaga aacactgaaa tatttttagg tgatggtgtt aagcgagcaa tgccttctga
     1921 acttagtaca aaagaagtat caagaaaaag cctagtggtc actgagaatt taaatgtggg
     1981 agatattctt acagagaaat ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa
     2041 gtttttatat caatatattg gcaaaaaagt taaaagacca ataagtgcag atagtgttct
     2101 gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa gtggaacacg agctgattat
     2161 ggaatatata aacctgttgc tgagaattta ctacaagata attgggatgt atcatttgtt
     2221 gtttctggaa tgcatttatc ccataaatat ggatttacta aagaattgat cttagctgac
     2281 ggatttagaa ttagtggaga agttcaaagt ttgtttttag agaacaataa gggaaacatg
     2341 gctcggtctg tggcattaga gataaatggt ttcacaaata tatttgaaca aactagccct
     2401 gattttgtat tattactagg ggatagagga gaaatgttgg cagctgcgat agcttgtttg
     2461 tatcttggaa tcaaaacagc acatattcac ggaggcgaag tgtctggtac tgtagatgaa
     2521 tcaataagac atgccataac gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc
     2581 aaagagcgtt taataaaaat gggggaagat tcttggagag tttttcccgt cggagctcct
     2641 agaatagata ctattttaaa taatagatta cctagtttta gagatataaa agaaaaatat
     2701 catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa
     2761 ctagaaaata tagaaaaaca aattttagct ttgatcaatt caatggaaca acaatctcaa
     2821 ccaattattt gcattttacc taatagtgat gctggtagcg aaattatcag aaaaatgtac
     2881 cagaaatatt taaaaacgga tattatttat attacgaatt tagagccgga ggaatacctg
     2941 actatattaa aagagtgcaa atttatgatc ggaaattctt cttctgggat aattgaagct
     3001 gcatcattca aaataccagt aataaattta ggaaccagac aaaatggacg agaacaatct
     3061 aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg aaactgcttt gaataaaata
     3121 caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg tgacggcaac
     3181 agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga agatattaag
     3241 tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt attattgggg
     3301 ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag ttaatatatg
     3361 gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg aaaataaacg
     3421 aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta gctattggtg
     3481 actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag caatatggaa
     3541 tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat ggaactgtta
     3601 tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct ataataaaca
     3661 caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta tctccaggtg
     3721 tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt ggttctcaat
     3781 gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc actattgtta
     3841 aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag aaagaaggaa
     3901 ttaattttga attctagaat tttattatca agtcctcata tgggaggaac agaagaaaaa
     3961 tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa tgtggataaa
     4021 tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc gttaagttca
     4081 ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc
     4141 tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca aggggctgta
     4201 ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc actagagagc
     4261 gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt agtgaattta
     4321 tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt taatgttcca
     4381 gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc gtcaggctta
     4441 tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac ctcaggtgga
     4501 ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt agcaacacaa
     4561 gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta ccgtctaagc
     4621 aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag agtagctaaa
     4681 cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag aattgaaatg
     4741 atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac gatcaatgat
     4801 tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa aaacattgaa
     4861 acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt aaaaatgtat
     4921 taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt atgcttacct
     4981 tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata
     5041 ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc catggggaaa
     5101 agaagactta gactcattca agaacacttt aatgatattt tactatgtgg tgttgatact
     5161 aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta taatagtcta
     5221 acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc ccctataacc
     5281 catgaattta ttataaaaga ggctttaaat ttagattgta atatctttac ggaaatcaat
     5341 ttattaaatg aatattatga tgaaataatc gaagtagcta aaaagaaaga tagattactt
     5401 tatctatctt ctacattttt gaagagaaaa gaaattcaat atattaagtc taaagtgacg
     5461 cataataaaa aaatttctta tcaataccat gtaggacagt atttacccga ctggcatcct
     5521 tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata
     5581 ttcgctattg aattaccttg gattatagat acatttggga aagttaaatc gtttactgta
     5641 atgacccaga aattatccaa tcttgatatt gattattcgg acagtttcac tcttattatt
     5701 gagcatgaaa ctggtatagt tggaacttta agtgtagatg ttgtttcaag agtagctaaa
     5761 agaaatttaa ctataattgg tgaaaagatt ttaatagaat gggaaggtaa cccggattct
     5821 ctattttcat ggaatgaaga aacttctagt atgacagata ttcaattgta cgatgaggtt
     5881 atgcatgata ataattattc acatactatt attgaagatg catatttaga ggagattaaa
     5941 gaatttttca cgcttttgga aaagaaaatg gaaccccaat atacttttga aaaagataag
     6001 tatattatta acttaattaa cgacattgag gtaaattaat gatgaaagta attttttttg
     6061 ggttagggtc aattggaaag agacatctaa aaaatctaat aagctatgga aaaaagaatt
     6121 ctattaattt tgatataaca gcttatagta gcagtggaaa aaaagagttt gaagaaataa
     6181 cgtatattca aaatagagac gatttggata cacattatga tatagctttt attacaaatc
     6241 cgacttttaa gcatctggaa acactaaatg taattgttga aaaagttgat tacatatttt
     6301 tagaaaaacc tgctttttct gagagtattg atcttactca atataattat gattacaaaa
     6361 atatttatgt tgctgctcca ctgaggtata aaaatatcat ggaagttgtt cgtaatagta
     6421 ttcaaagtaa aaaagtatat tctgtaagag ctatttgttc tacttatctt cctgattgga
     6481 gaaaaggtac tgattataga aataattatt ctgcctttaa atctatgggg ggaggagtag
     6541 aattagactg tatacatgaa ttggattata ttataaacct atttggtttc cctaaatcag
     6601 taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa cagtaatgat ttagcaacat
     6661 atatattgag atacgatgat aaaattatag aaattcattt agattatttt ggaagagttc
     6721 ctacaagaaa atttcaattg tttactttag atgaatgtat caatgttgac ttattaaata
     6781 ataaaataag ttttgaagaa aacattgtta aagaaatgcc tgaatctgct aatgatatgt
     6841 atgagaagga gttgaaatat ttctttgaaa acgtagtaac aggcaaagaa aactggaatg
     6901 atttatatca tgcaaacgaa gtattgaaaa tagctgagga gaaataaatg gaaattttat
     6961 ttactatttg tggtagagct gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg
     7021 caggaaagcc cctagtccat ttcgctttag caatgataga tatctatcaa caaaaacatc
     7081 ctgaacataa ttgcacgact tgtttgaata cggacagtaa tgagttagta gaattgacga
     7141 ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc tgaattagct acagatactg
     7201 tagggaaaat tgaagtgata cgtgattcat tcttacaact agaaagagaa gataatatat
     7261 ttgatttaat tatagattta gatttgactt cacctttacg tgaagtttct aatcttgaag
     7321 aattaataga tgaacatcta aaaaatttag ataaagacgt ctacttttca gttgtttctg
     7381 caagacgtaa tccctatttt aatatggtaa aaagaattga aaatgaagtt actatagtta
     7441 ataaatcaag atacacatct aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa
     7501 tgtattcttt taaacctgaa tttctaaaaa aaagtaatta tatttttgat gggcaatgtg
     7561 ggataataaa aatgagagat tatctaattt tagatattga tagtgaagaa gattttaaat
     7621 ggttagaatt tttgtttcct aaatttttag aagattcttc ggagcttcaa gatatctata
     7681 attatttata tcggaggtaa aaggatgagc tgttatctta tcaccggtgg ggctggcttc
     7741 attggttcta ccttagccaa tcatttagga gaagaacatg tggtaatcgt cgtcgatgac
     7801 ttatcaatgg gaaaagtaga aaatcttgat atgacgaaaa atatcacttt tattgaggga
     7861 gatgtcgctg agaaatcctt gatggaagag atcatgaaag ctaataaatt tgattatatc
     7921 tttcatttag cagcagttgc tagtgtggca gattcagttg cccgaccagt agaaacacat
     7981 cgtgtgaatt ttgaaagtgt attaatgatt ttagaattga tccgcaagta tcaacctgat
     8041 ttaaaacgga tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc gactttacca
     8101 aaaaaagaag agtccgtgat tcgcccattg acgccttatg cggttgataa attcgctgca
     8161 gaacaatatg tgctagatta ttgtcattta tatgatgtac ccggtagcgc agttcgtttt
     8221 ttcaatgtct atggtccaaa tcaaaatccg aattctccat acagtggtgt gatttctatt
     8281 ctcgtagatc gctataagaa acaaatcgta ggagaagcaa cttcatttac attatttggt
     8341 gatggaagcc agtctagaga ctttgtttat atagatgatg taatccaagc attactttta
     8401 gttgcgaatg aggaagctgc actggggaaa caattcaatg ttggaacggg taaagcaacc
     8461 acacttttgg aattgattca tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa
     8521 tacaaagaag agcgttcggg tgatatccat gactctctag cagatattac taagatccag
     8581 tcaattggct atcaaccgaa atatggtatt ttaagtggaa tgactaatta tttgaaaact
     8641 gaaatcaatt aaaacatact aagataagat tagagggtaa attcaggctc tttgtcaata
     8701 aggactgatg agttttgtaa aatcaaatct gggatagaa
//
LOCUS       DAZKAY010000106         8602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000106 DAZKAY010000000
VERSION     DAZKAY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.109"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..751
                     /locus_tag="ACG8WP_002420"
     CDS             <1..751
                     /locus_tag="ACG8WP_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9028689.1"
                     /translation="FNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEHAVKLR
                     KEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQEWNAQI
                     QKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHVDYFEP
                     CFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGSSFVYP
                     RTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            738..2179
                     /locus_tag="ACG8WP_002421"
                     /pseudo
     CDS             738..2179
                     /locus_tag="ACG8WP_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            2190..3521
                     /locus_tag="ACG8WP_002422"
     CDS             2190..3521
                     /locus_tag="ACG8WP_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9028690.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            3534..3992
                     /locus_tag="ACG8WP_002423"
     CDS             3534..3992
                     /locus_tag="ACG8WP_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9028691.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            4284..5585
                     /locus_tag="ACG8WP_002424"
     CDS             4284..5585
                     /locus_tag="ACG8WP_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9028692.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            5612..5872
                     /locus_tag="ACG8WP_002425"
     CDS             5612..5872
                     /locus_tag="ACG8WP_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9028693.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(6871..8106)
                     /locus_tag="ACG8WP_002426"
     CDS             complement(6871..8106)
                     /locus_tag="ACG8WP_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9028694.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         2695 a         1310 c         1785 g         2812 t
ORIGIN      
        1 ttttaattcg gatgattttg gctatagcca tggtgtgaat tatgggatta tggatgctta
       61 tcagcgagga attttgacat caactacatt gatggcaaat atgccgggtt tcgagcatgc
      121 tgtgaaacta agaaaggaaa tgcctaggct aggtgtagga gtccatttaa cattgacgtg
      181 tggcaaacca ctcctcaaaa ctgtcgatac tcttatcgaa agcggagagt tcagatcctt
      241 atcttattat cagcatccat ttaacattga caaagatcaa ctgtatcagg aatggaatgc
      301 tcagattcaa aaaatttatc atgcggggat cataccaact catttagaca gtcatcatca
      361 tactcatact ttggatgaaa atcaggaggt agttgttaca ttagcaaaaa aatatgattt
      421 gccggttcgg agcaactttg aacgaaaaga tgaagttcgt catgtcgatt attttgagcc
      481 ttgtttcgat gtcgtaggag aaactgatga aaaaaaacga caaatcaata cgagtgtgga
      541 agaatatttg gcaagtttac tcatgatatt gagagaagat aaaacgatcg aagtcatgtg
      601 tcacgcagcg tatttggatc aggaattgtt aaatggttca agttttgtgt atcctcgtac
      661 ccaccaagtc gaatttttaa ttcattcagc atttgctcaa aaagtgaaaa atgatccttt
      721 gattgagtta gtaacatatg aggatattta atagaggagg aataaaaatg aatgttaaat
      781 caaatgtgca gaagcttgga agagcgatgc tacttccagt tgcagcgatg ccattagcgg
      841 gattgatcat gcgtttatca gctgacgata tgctgaacat cccagtaatt ggtgcagctg
      901 gaaatgccgt ttttggaaac ttggatatct tatttgctat aggagttgca atcggttttg
      961 tcaaaaataa ggacaaagga atacctgcac ttactggttt tttagcgatt gctaccttga
     1021 aaaaaggctt agaaattatg gacccagaag tgaatatggg gatttttggt ggaattattt
     1081 ccggtttagt agcggcatgg acgtataatc gctttaaaga acaaaaatta ccagtagttt
     1141 tttcttattt tgcgggcgaa aaattcccat tgactatggt gatgattcta caaacaatcg
     1201 cagctgtttt tttgggcttt atctggccgc tcatccaaat tgggattgat aattttgcaa
     1261 aactgctcgt tgatatggga gcattaggtg tggggatatt tatgtttttg aatcgtttgc
     1321 tgatcccctt tggactgcac catgtgttga atacgtatat ttattacgat ttaggaagct
     1381 atacatctcc tagcaaggaa gttttcagag gagagatgac acgctttatc aacggagatc
     1441 ctcaggctgg actgttttta tcagtttttt tgtagtaatg atgtttggta taccagctat
     1501 atgtctagct atctataaag cagcttataa agaaaataaa gagatggtca aaggaatcat
     1561 gggaagcggt gcaggaactt catttattgc aaatatcact gagcctgttg aatttagttt
     1621 catgtttctt tctcctatgt tgtacgtgat ccatgcactt tttgccggtt tagcagggat
     1681 tgtctgttac ttattcaata tacgaattgg ttttacattc ggagcatgta ttgttgatta
     1741 tctcatcaat tttcgaatcg ctaccaatgc tattttgatt cttccaatcg gaattttctt
     1801 ttttgccttg tattacgtta ctttttatta tttgatcaat aagagaaaca ttcagacatt
     1861 aggacgtgaa gctaaggcag agtttggcaa tgaagtaacc ttagaagaaa cagagttagg
     1921 gttagcaagt aaaaattatg aatatatggc aacaaaaatg ctacaagcgt ttggaggaaa
     1981 agccaatatc ctggatgtct atagttgtaa tacacgccta cgggtagagg tagtggatcc
     2041 caccatggtc gaggaacagc gaatcaagca attagggata agcgggatca ttaaaccaac
     2101 tgaaaaaaat tatcaaatta tcatcggctt agaagtgact tatgttatgg cagaattcaa
     2161 taaattatta gaagaataga aaggaaaaga tgatgatgaa aaaacagatt atcactatag
     2221 ctggaggcgg gagtacgtat acaccaggaa tcgttcaagc cattttgaac aatgaaaaac
     2281 ggttgccatt atctgaaata cgtttatatg atattgatga agaaagaaat atggatatgt
     2341 acttgattgt taaatttatg ctgaaaagaa aaggtttttc aaatattcgc attcgtgcaa
     2401 cggatgatcc aaagcttgct tttacaggat gcgattttgt tttctcacaa attcgtgttg
     2461 gaggattgga gatgcgagaa aaggacgaaa agattccact taaacatgga ttagtaggac
     2521 aagaaacgtg tggactcgga ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg
     2581 aaattgtcga tcatatacaa gactatgccc ccaaagcatg gattttgaat tatacaaatc
     2641 cagagtcgat cgtttctgaa gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg
     2701 cctgtgatat gactatttca attgaagaaa cgattgcagt gaattacggt tatgatcgta
     2761 agaattggat cgttacgtat tatggtttaa accactttgg ctggtataca tcgatttatg
     2821 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
     2881 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
     2941 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
     3001 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
     3061 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
     3121 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
     3181 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
     3241 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
     3301 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
     3361 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
     3421 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
     3481 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
     3541 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
     3601 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
     3661 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
     3721 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
     3781 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     3841 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     3901 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     3961 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     4021 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     4081 atcattttga tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt
     4141 attttcagga ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt
     4201 cttaattata taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat
     4261 tttattgaaa aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg
     4321 tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg
     4381 tttcaacatt gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt
     4441 ttcctggatg gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga
     4501 tgacgatggg attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat
     4561 catatggatt agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac
     4621 aagttcctgt aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga
     4681 gttctttggg agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag
     4741 tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg
     4801 ctgttgcccg tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc
     4861 tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt
     4921 taaccaatat tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt
     4981 tgttgtggtc atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt
     5041 ggctgatcat gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat
     5101 taccctatat cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag
     5161 caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg
     5221 ttggtcgttt ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag
     5281 ctcctatgat gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga
     5341 ctattatcag ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa
     5401 catggacatt accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg
     5461 ttatcttgat catgatcaac acattgatag catttattat ctattatcca tttgtaaaag
     5521 cttatgataa aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg
     5581 tctaagtttg gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt
     5641 tatcttttta ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca
     5701 aatcaatcga tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat
     5761 tgtaccgcat atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc
     5821 tatcaatttt atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta
     5881 aaagtggttg taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc
     5941 ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga
     6001 tgcaagcttt tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag
     6061 ccctttttta cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa
     6121 aatggaaatt ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag
     6181 tcaaatatag atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat
     6241 ggtaatatgt atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg
     6301 ctgggaatag caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata
     6361 gaacattctg acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat
     6421 gcatcggatt gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag
     6481 tggtaaatcc tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca
     6541 aagcgatgct tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat
     6601 ggtctttctt ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg
     6661 actaagaata aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt
     6721 taaggatgat tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca
     6781 ctatttactt tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt
     6841 ctctattaaa aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc
     6901 gatttcttga attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc
     6961 ctgctgacga atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt
     7021 cactggtttt gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct
     7081 ttgctcaaat tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac
     7141 ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga
     7201 cagatattct gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc
     7261 aataggtgca ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa
     7321 catacgcgga ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg
     7381 ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat
     7441 tggaccaacg attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt
     7501 tgtgatgaga tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat
     7561 ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt
     7621 ttttgtccag tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat
     7681 cagcgcatgc tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa
     7741 aggaatcttt ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag
     7801 tcgaggctca aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc
     7861 tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg
     7921 ttttttcgta aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg
     7981 agcaaaagtt ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc
     8041 aacctcaatc attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt
     8101 cttcatgaaa tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa
     8161 aaaacacagc gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt
     8221 tcacaacttc taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac
     8281 gcaacaattt tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt
     8341 aaaaataaac atgagaacat aaaaaaataa aataattata ttaattgata ggtgtgctgt
     8401 cgtcattata cacacgtaag aaagaaatag ttatgtttaa aaatattctt agactttctc
     8461 gccatgacaa ttttcactat gtgttataac tgtaaacgat aattattcag ataacacaaa
     8521 aagaaatcaa ggagcagata cgccagttgt ttaatttgaa tattatttta aaattaaaat
     8581 ttttattctg ttctaaacat tt
//
LOCUS       DAZKAY010000107         8409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000107 DAZKAY010000000
VERSION     DAZKAY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.110"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..1063)
                     /locus_tag="ACG8WP_002427"
     CDS             complement(95..1063)
                     /locus_tag="ACG8WP_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9028695.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1295..2128
                     /locus_tag="ACG8WP_002428"
     CDS             1295..2128
                     /locus_tag="ACG8WP_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9028696.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2162..3001
                     /locus_tag="ACG8WP_002429"
     CDS             2162..3001
                     /locus_tag="ACG8WP_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9028697.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3027..3587
                     /locus_tag="ACG8WP_002430"
     CDS             3027..3587
                     /locus_tag="ACG8WP_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9028698.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3786..5507
                     /locus_tag="ACG8WP_002431"
     CDS             3786..5507
                     /locus_tag="ACG8WP_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9028699.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5672..6814
                     /locus_tag="ACG8WP_002432"
     CDS             5672..6814
                     /locus_tag="ACG8WP_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9028700.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6830..8041
                     /gene="thiI"
                     /locus_tag="ACG8WP_002433"
     CDS             6830..8041
                     /gene="thiI"
                     /locus_tag="ACG8WP_002433"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9028701.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2885 a         1455 c         1743 g         2326 t
ORIGIN      
        1 tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc
       61 tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt
      121 cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat
      181 taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat
      241 cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt
      301 ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag
      361 cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca
      421 tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct
      481 ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac
      541 ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg
      601 cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac
      661 ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg
      721 aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa
      781 aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg
      841 cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg
      901 agggaagatt gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg
      961 catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca
     1021 tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac
     1081 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     1141 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     1201 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     1261 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     1321 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     1381 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     1441 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     1501 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     1561 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     1621 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     1681 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     1741 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     1801 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     1861 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     1921 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     1981 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     2041 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     2101 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     2161 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     2221 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     2281 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     2341 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     2401 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     2461 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     2521 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     2581 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     2641 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     2701 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     2761 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     2821 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     2881 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     2941 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     3001 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     3061 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     3121 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     3181 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     3241 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     3301 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     3361 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     3421 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     3481 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     3541 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     3601 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     3661 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     3721 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     3781 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     3841 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     3901 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     3961 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     4021 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     4081 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     4141 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     4201 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     4261 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     4321 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     4381 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     4441 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     4501 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     4561 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     4621 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     4681 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     4741 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     4801 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     4861 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     4921 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     4981 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     5041 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     5101 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     5161 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     5221 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     5281 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     5341 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     5401 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     5461 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     5521 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     5581 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     5641 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     5701 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     5761 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     5821 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     5881 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     5941 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     6001 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     6061 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     6121 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     6181 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
     6241 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
     6301 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
     6361 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
     6421 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
     6481 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
     6541 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
     6601 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
     6661 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
     6721 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
     6781 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
     6841 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
     6901 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
     6961 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
     7021 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
     7081 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
     7141 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
     7201 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
     7261 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
     7321 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
     7381 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
     7441 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
     7501 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
     7561 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
     7621 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
     7681 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
     7741 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
     7801 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
     7861 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
     7921 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
     7981 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
     8041 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
     8101 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
     8161 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
     8221 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
     8281 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
     8341 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
     8401 ttgcgaaaa
//
LOCUS       DAZKAY010000108         8252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000108 DAZKAY010000000
VERSION     DAZKAY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.111"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..1560
                     /locus_tag="ACG8WP_002434"
     CDS             235..1560
                     /locus_tag="ACG8WP_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9028702.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1553..1903
                     /locus_tag="ACG8WP_002435"
     CDS             1553..1903
                     /locus_tag="ACG8WP_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028703.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1900..2070
                     /locus_tag="ACG8WP_002436"
     CDS             1900..2070
                     /locus_tag="ACG8WP_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028704.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2974..3249)
                     /gene="prgO"
                     /locus_tag="ACG8WP_002437"
     CDS             complement(2974..3249)
                     /gene="prgO"
                     /locus_tag="ACG8WP_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9028705.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3221..4174)
                     /locus_tag="ACG8WP_002438"
     CDS             complement(3221..4174)
                     /locus_tag="ACG8WP_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028706.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4786..6279
                     /locus_tag="ACG8WP_002439"
     CDS             4786..6279
                     /locus_tag="ACG8WP_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9028707.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6414..6710
                     /locus_tag="ACG8WP_002440"
     CDS             6414..6710
                     /locus_tag="ACG8WP_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9028708.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6811..7662
                     /locus_tag="ACG8WP_002441"
     CDS             6811..7662
                     /locus_tag="ACG8WP_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028709.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7683..7895
                     /locus_tag="ACG8WP_002442"
     CDS             7683..7895
                     /locus_tag="ACG8WP_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028710.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2866 a         1283 c         1553 g         2550 t
ORIGIN      
        1 atttttttct aatataacta agcaaaactg gataatacga cacttatttt caaattttca
       61 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
      121 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      181 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      241 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      301 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      361 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      421 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      481 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      541 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      601 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      661 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      721 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      781 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      841 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      901 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      961 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
     1021 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1081 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1141 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1201 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1261 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1321 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1381 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1441 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1501 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1561 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1621 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1681 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1741 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1801 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1861 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1921 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1981 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     2041 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2101 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2161 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2221 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2281 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2341 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2401 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2461 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2521 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2581 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2641 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2701 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2761 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2821 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2881 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     2941 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     3001 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     3061 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     3121 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     3181 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     3241 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     3301 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     3361 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     3421 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     3481 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     3541 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     3601 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     3661 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     3721 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     3781 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     3841 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     3901 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     3961 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     4021 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     4081 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     4141 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     4201 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     4261 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     4321 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     4381 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     4441 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     4501 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     4561 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     4621 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
     4681 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     4741 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     4801 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     4861 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     4921 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     4981 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     5041 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     5101 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     5161 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     5221 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     5281 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     5341 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     5401 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     5461 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     5521 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     5581 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     5641 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     5701 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     5761 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     5821 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     5881 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     5941 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     6001 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     6061 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     6121 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     6181 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     6241 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     6301 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     6361 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     6421 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     6481 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     6541 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     6601 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     6661 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     6721 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     6781 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     6841 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     6901 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     6961 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     7021 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     7081 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     7141 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     7201 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     7261 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     7321 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     7381 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     7441 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     7501 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     7561 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     7621 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     7681 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     7741 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     7801 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     7861 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     7921 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     7981 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     8041 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     8101 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     8161 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     8221 tcgtaagaga aattgatcga agatgtgacg aa
//
LOCUS       DAZKAY010000109         8157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000109 DAZKAY010000000
VERSION     DAZKAY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.112"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..579
                     /locus_tag="ACG8WP_002443"
     CDS             <1..579
                     /locus_tag="ACG8WP_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028711.1"
                     /translation="AILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILAL
                     LMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIA
                     ASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYL
                     TDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
     gene            663..1751
                     /locus_tag="ACG8WP_002444"
     CDS             663..1751
                     /locus_tag="ACG8WP_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFP9028712.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1748..3169
                     /locus_tag="ACG8WP_002445"
     CDS             1748..3169
                     /locus_tag="ACG8WP_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9028713.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            3205..3921
                     /locus_tag="ACG8WP_002446"
     CDS             3205..3921
                     /locus_tag="ACG8WP_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9028714.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3940..5523
                     /locus_tag="ACG8WP_002447"
     CDS             3940..5523
                     /locus_tag="ACG8WP_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFP9028715.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            5652..6236
                     /locus_tag="ACG8WP_002448"
                     /pseudo
     CDS             5652..6236
                     /locus_tag="ACG8WP_002448"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            6723..7748
                     /locus_tag="ACG8WP_002449"
     CDS             6723..7748
                     /locus_tag="ACG8WP_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9028716.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            7997..>8157
                     /locus_tag="ACG8WP_002450"
     CDS             7997..>8157
                     /locus_tag="ACG8WP_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9028717.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2969 a          923 c         1535 g         2730 t
ORIGIN      
        1 gcaattctag tgtattatgt tttattaaag tggggaaaaa agattcaaat ccgtgcatta
       61 gtgtgtacta tttgtgcaac tgttatttcg ttagtgttta tttacattat agatacagga
      121 attttggcat tgcttatgtc aaatttgggc attacagatg acggtgcaag gctttcattt
      181 tatctatatt catctaaatt ttatgaactt ggtttaggat atgtaggaac aggatggaca
      241 tggttttcaa aatacttttt taatctttat ttatcgcact ttaggttaaa tggtggaaga
      301 attgcagcaa gcctacacag tgatatatta actatttata tagaagttgg atgtatagtt
      361 tttattatat ggatgttttg gttatttttt gtgaaaccga taagactaac taaaaaattt
      421 ggattgaaaa caggggagtg tgtattatta ttaactatat atatgtttat attatatttg
      481 acggataata cgctcactta tccagacact caaatgattt ttctcttagt acctatgata
      541 gtttcgtggg ttgataatca acaacgaata tcggaataga tagctaaagt atattttaat
      601 tatgaattta taattagttg taaaagttaa tatttaacgg acaggaagta gggtgaaaaa
      661 atttgaaaaa aattttagtg ttagcaaata catattatca aataatagta gcaatacaaa
      721 tgaaaaatac aattttttcc gatgagtatg ttgaaattat tatttctgat cattctaaaa
      781 acactaaaga aattgcgaaa aaaatagaag aaaaaaaagt gtttgatggc tgtcattatt
      841 tagaaagcaa atcttttgat gataatattg ggtattttca aaagattaag gactgtcttc
      901 agatttcttg tggtaaatca aatcgatatg cctattattt gaaaaactgt aaaagtgaat
      961 attttgatga atttatttgt ttttcaatgg gagaggtttg gttggaagga atatatgctt
     1021 gcttatatcg aaataataaa aatgtacagg tatcaatttt agaagaaggt attttaagct
     1081 atggagttga cattattcca acagcaagaa caaaagctat cgcagtactc aattacttaa
     1141 aaaaaaggac tccactatat aacaaaagct ataattttta ttgttttttt cctaatatat
     1201 atacaggaag agagaataca atacaaattc cattagttac agctgggtca agaaccgcat
     1261 tgcaactttc ggatattttt aaccctatga tagatattta taaagaaaaa aaatatatat
     1321 tttttacgtc tgtttatgat tttgagggcg gagaacctgt cggagaatat gaattagtat
     1381 gcaaaatagc agacttagta ggaaaagata atctaattgt taagacgcat cctagagatg
     1441 taagaacaat ttataaagat aatggattta tcgaggataa aaattcatca ataccttggg
     1501 aggcgattca attgtcaggc gattttaacg ataaaatatt tatgacaatt aattcaggaa
     1561 gtgttatttc aggtagtaca atgtctgcaa aacctgtacg cacgtattat atgtataaat
     1621 tgtgtaacat agaaggaaat ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc
     1681 ttaagcaaaa aacgatgaaa aacattttaa agaatgtgaa aatagcagaa agcttagatg
     1741 atatactatg agcaaaacta aatatttaat taaaaatatg ggaatattaa caataagtaa
     1801 tttttcttca aaaattttag tttttttgtt agtcccttta tatacaagtg tattatccac
     1861 aacagaatat ggcacatatg atttgacaat ttcaacagcc acattattgt ttccaatttt
     1921 aaccttaaac attgtagatg ctgtgatgcg ctttttaatg gataaagata aaaataaaaa
     1981 aaatatagca tctatagcga ttaaagttat atttttaagc gtgatagtct ttggatccgt
     2041 aatgtatttg ttaaatatta caaaattatg gcaagatgtt tatggattag aaaaatatat
     2101 ttttttgtat tatatatcct atgtatggaa tcaatttcta atacaatttg ctaaagggtt
     2161 agaaaaagtt aaagctatgg gcatagcagg tattatcagc acaattgtaa caataattgc
     2221 caatattttc ttcttgctta ttctgaaatg gggattaatt ggttttttca ttgcaaatat
     2281 tttatcacag attatttcag ctacttatct gtcagtatta gtgagaatat gggaatatat
     2341 cgattttgag gctaaagatc ctcagctaaa gagagaaatg ctaaattact cgattccatt
     2401 aattgcaact gttgttggat ggtggattaa tagtacagct gataggtatg tggtttcctt
     2461 tatagtaggt gtctcggcga atggcttatt gtctgtggca tataaaatac ctcaaataat
     2521 taatactttg cagggaatat ttatacaggc ctggcaaatt tctgcaatta aagaatatgg
     2581 agataatgag actgcaaaat tttatggaaa tacatttgtt atgattaatt tgttaatgtg
     2641 cataacctgt tcaagtctta tcatattaac tagaccgttt tcacatattc tttatgcgaa
     2701 agacttttat gaagcgtgga aatatgttcc atttttatta atagctaatg ttttaaattg
     2761 tgcatcaggt ctgttaggac ctatcttatc agcaaaaaaa gattctaaat ccatgatgtg
     2821 gtctgcaatc ataggggcat tagttaatgt gattatgagt gtagtccttg tttataagat
     2881 aggggttcaa ggtgccacaa tagccactgt tatatgttca tatgtaatat atgttgttcg
     2941 taaacgagct gtgggaaaag atatttttat aaagcattat catattgttg tggaatcttg
     3001 gggattattg tttgttcagg caatcataga aatatatttt aaaaattatg taatagaaat
     3061 tataattatg attattttaa ttattataaa tatcagttat attaagcagt tgtgttatag
     3121 aggaagaaac attatttcaa tgattaagag tcgagagcac aaacggtaga cgatgttatt
     3181 tttataaaaa gatataattt attaatggtc gataattatg aaaggaaaaa tattcttaaa
     3241 aaaaagagta taggatgtcg tatgaaaata gtcgctatag taccaatgaa gttaaataac
     3301 aaaagattgc cacaaaaaaa cacgaaagct tttaccaatg gaaaaccatt gtgttattat
     3361 atactttcaa cacttttaaa agtaaaacag attgataaag tgtatgtcta ttgtagtaac
     3421 ccagatatta aagaatttat accagaaggt gtaacatttt taaagagatc tagttcctta
     3481 gatcaggata caacgagtat tacacaggta ttagccggtt tcacaaaaga agtagaggct
     3541 gatgtatatg taatgacaca cacaacagca ccatttatta gcgaaaaatc tattgaaaaa
     3601 ggactagatg ccgtgctgac aggagaatat gattctgcat ttgctgcaaa aaaattgcaa
     3661 gatttcttat ggaaagacgg tgaaccattt aattatgaat tggacaatat tcctagaaca
     3721 caggatttgc cgcctttata cgaagaaact agtggatttt atatttataa tcgcaatgtg
     3781 atgaccgagt tgcatagaag aattgggaaa aaaccttgca tagttgaagt aggtgagatt
     3841 gagtcagttg atattgatga agccgaggat tttatgattg ctgatgcgat ttataatcat
     3901 cttattttac aaggtagtta agacaggggg aaggttagga tgaacaatgt acagatttta
     3961 gattgcacac ttagagatgg gggctattgt aacaattggg aatttggatt tgataataca
     4021 aaaaaaatta tttctggact tatagaatca ggaattgaga ttatagaatg tgggtttctc
     4081 acaaataaaa cagtatacaa taaaaatgtt acaaaatata cgaccttaga agaattagca
     4141 cagataattc ctgaaaaaag agaaggaaaa ttatttgtag caatgatgaa ctatggcgaa
     4201 tataatatag aggatcttcc agattatgat ggttcatcta ttgatggaat taggattgcg
     4261 tttcataaaa aaaattgcaa tgaagcgttg aaattatgtc gtgagataaa gaaaaaaggc
     4321 tacttagttt ttgttcaagc tatggtatct ctcaattata cagatgaaga gtttttagca
     4381 gtaataaaaa atgtgaatca gtttaaacca tacgcttttt atattgttga tagttttgga
     4441 atgatgaaag gaaaagattt aactcgtctt ttttacatga ttgagcataa tctagaaaaa
     4501 gatatacaga taggttttca ctcacataat aacatgcagc ttgcatattc taatgcacaa
     4561 aaacttataa ccgtacaaac aaatcgaagc ctgattattg attcttcggt gtacggaatg
     4621 ggaagaggag caggtaatct taacacagaa ctatttgtag aatatttgaa tgataatgca
     4681 ggaaaaaaat atcaattaaa accattatta tatataattg atgaaatact aaatagtttc
     4741 tatcagaaga gttattgggg atattcatta ccaaactata tttcagcagt tcataatgct
     4801 catcctaatt atgcaagcta tctgtcatat aagaatactt taacaattga agcaatgaat
     4861 gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt atgataaaca atatatagaa
     4921 gatttatact acaaatatat ggaaactggg aaaatacagg aggaatatat ggaagaactg
     4981 agaaaatttc tctcagacaa aaaaattcta ctgattgcac caggaaaaag ttctgtagat
     5041 gaggtagaaa gaataaaaga gtttgttacc aaagaagaag tcgtaataat tagtgtaaat
     5101 tttgaatatt ctcatataga tatagattac gtatttttaa gtaatttaag acgttttcat
     5161 gaattaccga aagaaagcag aactaaatgt attgttacat caaatataca agcatcggat
     5221 gtatatttac ggacgagtta taaaaagttg ttatctgaaa gggaagttgt aagagacaat
     5281 gcaggattaa tggcaattag atttttagcc gatatgaatg tggatgaaat attcttagct
     5341 ggatttgatg gatattcaca taatgaagat gagaattatg gtgagcaatc catggaaatt
     5401 ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa tgagagatga attgtcagaa
     5461 ttagctaaaa aagtaaaggt gacattttta acggatccac gttgggttcg aattgataag
     5521 tagtagtaat aatgttttta aaattttgat aaagagatag tgagagaata aaaatgggta
     5581 aagtcttaaa taaaaaagaa gaaaaatatt taagggccga tggggcgaga tgaaactcta
     5641 attttgaact tttaagaatc atttctatat tgtgtattgt tgcttaccat gctaatttgc
     5701 gtttaaatta taatgcagac ggaattatag tacatggttc attaactgct aaccagattt
     5761 tcttgaatta tattggtatt tggggtcagc taggagttaa ttgttttata tttatttcgg
     5821 catatttttt aagtgagaaa agaaacattc actttgaaaa tgttttggga attgtattag
     5881 aatgtatttt ttatagtgta ttagtttatg gaattctttg tgcatgtgga atagaaacat
     5941 tttcattgaa cagctttgta aaagaaatat tatcatttgc tctaggtaga tattggttta
     6001 ttacatccta tttagcattg tatattctaa gtccgtattt aaatatcatg cttaacaatc
     6061 tggaaaaact tcagttgcaa aaaataagta tgttattgtt tataggtgta tgctgttatc
     6121 gtatgttatt taaaacatct ttgtttgggg attgtgctgt tttaattgca ctatatgtca
     6181 tatgttatac actgaaaaaa acgataataa tatttttgag agacacagaa tcataggaac
     6241 aattgtatgt ataacagtta taccacttct catatccata ggaatacttt tcaaatttat
     6301 accaaatgca atggttaacc agatggttca tattggttct ccattgatgg ttatagcaag
     6361 tatattttta ttttatgtat ttaaaaattt ttcattttac agtaagtata taaataaact
     6421 tgcgagaacc agtttagggg tatatatttt acatgaaaat cattatttca ttgatggtat
     6481 gaagtctaga ggcttaatat gggatgggat atttggatta ggcaaaaggt ttttagatag
     6541 ggatttttgg atttggttta taattgcagt tgtagttgtg tgcataattt gcatggctat
     6601 cgatgttgta agagaaaaat ttgaaagaca gatttttaaa attaagagtg taaagaacct
     6661 gtgtaaactt atgaatcatg ttataaatat atacccataa ttaataaatg aggagaaaag
     6721 aagtgaaagt tatatcattc ggagaattat tattgagact tgcagcaccc ggttattcaa
     6781 agttatttca aagagatagt ttggagacta cattctgcgg aggggaagct aatgtagcgg
     6841 tatccttagc aatatttgga tatcaatcag aatttataac aaagcttccg gattcagatg
     6901 ttggatatgc ggcagctaat gctttgcgtt attttggagt aaatgttgat aatattatat
     6961 ttggagaggg acgcatggga ctgtattatt tagaaaaagg agcagctcag agaccatcta
     7021 aagtaattta tgacagaaaa ttttctgcaa tttcgttagc tgatagtgat gaatttaagt
     7081 ggaaggagat cctaaacgat gcaaattggt ttcattggac agggataaat ccagcattgt
     7141 ctgagaatct ctttgagacg tccttagatg catgtcaggc tgccaaaaaa agaggtataa
     7201 caatttcttg tgatttaaat tatcgatcca agctttggac atcagaggaa gcaaaagaaa
     7261 agatggaaca gatactacca ttggttgata tttgtatttg taatgaagaa gatgcagaaa
     7321 aagtgctagg aattaaagct gagcatacaa atattaatag tggagaatta aataaagatg
     7381 gctatgtgca ggtggctagg gagattcaca agaagtttgg atgtaggtat gtagctacaa
     7441 ctctgcgtag aagttattcg gcaagtagaa atggttggag tgcaatgtta tacgaagcag
     7501 aaaaagataa agcgtatttt tccacagaat acaacataca aattgttgat cgcgttggtg
     7561 gtggagacag cttttctgct ggtataatat atgcgttatg tgaaaatatg tccggacaaa
     7621 aggctattga atttgcgact gcatctagtt gtttaaaaca tactatagaa ggtgatttta
     7681 atcgaactac aattgaggat gtatataatt taatgaatag cggtggaaat ggtcgcgtgt
     7741 tgcgttagtc aacatataaa gataaatcac agtaattttt atggtgatta tctgtcttag
     7801 tagtgattag aataacgtga aaataaattg ttgagcgttt atactgtttt gaaaaataaa
     7861 tttaacatat tgaaaaatac aaatatatac gtatattata aagcgccgat tgtaaaatta
     7921 agctagacaa ctaaaacagt catttgtgct acactcaaaa tagttccgac caaagaatta
     7981 taaggagtga gtgcaaataa cctataccta tcttacaacg gatgaacttg taataataga
     8041 gtcttatttg aaaattaacc aatctgttgc taaaactgcg cattgcttga atcgttcaag
     8101 tcaaacgatc cataaagtat atctgttctt caagcaaaga aaatcagcct tagaata
//
LOCUS       DAZKAY010000110         7975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000110 DAZKAY010000000
VERSION     DAZKAY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.113"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1659
                     /locus_tag="ACG8WP_002451"
     CDS             22..1659
                     /locus_tag="ACG8WP_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028718.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1804..2544)
                     /gene="truA"
                     /locus_tag="ACG8WP_002452"
     CDS             complement(1804..2544)
                     /gene="truA"
                     /locus_tag="ACG8WP_002452"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9028719.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2735..3370)
                     /locus_tag="ACG8WP_002453"
     CDS             complement(2735..3370)
                     /locus_tag="ACG8WP_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9028720.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3517..4737)
                     /gene="sstT"
                     /locus_tag="ACG8WP_002454"
     CDS             complement(3517..4737)
                     /gene="sstT"
                     /locus_tag="ACG8WP_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9028721.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4956..5318
                     /locus_tag="ACG8WP_002455"
     CDS             4956..5318
                     /locus_tag="ACG8WP_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028722.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            5305..>7975
                     /locus_tag="ACG8WP_002456"
     CDS             5305..>7975
                     /locus_tag="ACG8WP_002456"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9028723.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2613 a         1493 c         1238 g         2631 t
ORIGIN      
        1 acttttaaat aggagaaaaa aatgaaaaaa aacaaaaacc aaataataga tatcattttt
       61 atgttatttc taagttttgc ttacgtcatc cctctattca tgtctatggg aatctatcat
      121 agtgtcaatc aggatactta ctttcattta tctcgaatca taggactaga taatgtatgg
      181 agttctccag taaacttcaa taacttcgat catcatggaa caatgatgaa tattttttat
      241 ccatggctta ctctttatcc agctttttta ttttataaaa tgatgggaaa tcttgtttta
      301 agttataata tctactattt ttttatcact tttttaacaa tggtcgtttc ttatttttct
      361 atgaaacaaa taaaaaacaa tcgttatatt tcgctgttat tttcgattat ttatacattt
      421 tccgcctatc gtgccattga catattccat cgtgcttcat tgggagaagc tgtcgcttta
      481 acatttttgc ctttgatact aatggggtgt tacgaaattt atattagaga ttaccaaaaa
      541 tggtactggt tgtctattgg catgacgcta gtagtgtata ctcatttact ttctgtcgct
      601 atggtttctg tatttattgg aggaacgttg tttctttctt tttatttttg ggaccaaaaa
      661 atcgctagac tcttaagtct tttaaaagct accgtcctca cctttttttt aagtgcggga
      721 ttcttaattc cttttattca acaatctaga gcacaagaat tgaaagtccc actgggaaaa
      781 gaattaagtg gtatggctcc tagtgatatg ctgacacaca ttttaaataa taactataac
      841 aactatacta ttggattatt cttatttctc ggattgattg gtgcttttat ttttataaaa
      901 aaattaactg ctgatgattt atttattttc tttcttggtg tatttgttct tttttgttca
      961 accaatttat ttccttggca actttttaac catacaccag ttaagagtct acaatttgtc
     1021 tggcggttaa atggtttttc ttctttattt atagcctata cgatgagtat agtgatctat
     1081 tatatttttt ctactaaaaa taattcatgg aaaataatgg tctttacgtt tcttgcacta
     1141 atattgcatc tctcaggagt aagcaattct atccatgcta acaaagattc attaacactt
     1201 atagcacctg aagacgctgt tgctatagct gaaaattaca atcatactga ttatgcaaac
     1261 aaagagtcaa tctatcatcc tgatatgatt aataatgatc aatatttact aggtaaccag
     1321 gaaataaatc caacaactca ttttatgagt aacaaactta caattgaatt agataattcg
     1381 aataataaaa atactgtctt gacgacacct atttatcgtt ataaagggca agtggcttct
     1441 attaatggca aactagtaca aacaaaatta tctaagtttg gcacaactga attaactatt
     1501 cctccaggta taaataaagt cgttattact tatcagtaca caaaactagc aattgcttca
     1561 agatatttat ctatcgttac tttgatttta tttttacttt atcgatttac tttttctaaa
     1621 caaaaacaac cgagaagaga agtacaacat tctcactaga tttttattta agaaagactt
     1681 tttagctgta cacataaaca ggctaaaaag tcttttttta tatttcttta atttgcttca
     1741 tacattattt aacatcgata tttatactgt gaaacatgcg tttcatccat tatcaaagta
     1801 tttttatcga taataaactt catccaaaaa cagaccttgt gcagggacgg tttctcctgc
     1861 atgttgccgc actttgcttt caaatatttc atcgattgtt tctatcggca tggcacctgt
     1921 gccgatttct agtaatgtcc ccatgataat ccgcaccatt ttatataaga atccgtctcc
     1981 gacaaaagta aaatgaagca tttctccctt tcgttcgata gataattcat caatcgtacg
     2041 cactgtggat ttttttgatt ttttcaatgc agaaaaacca atgaaatcat gtgtacccac
     2101 taatttttcg caagctgcat tcagcttgtc catatccaac tcttgaggga aataaaagct
     2161 gtggtttcgc tcaaatgctg aaggaatcac gttgttccaa acataatagc tgtatttttt
     2221 gcccaccgca ttatatcttg aatggaagcg ctcaggcatt tcttcgattc ttttgatgac
     2281 aatatcccgc ggaaggtaac gattcataaa gtcgatcatt tctgccagtc caatttctgt
     2341 gtttgtttta aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga
     2401 tccaataatt tctatctctt catttgtcat ctgatgcaat actttttcga tttttccttg
     2461 aatggtttta tcgtcatctc caagtctttg ccatcctgaa tatctttttc cgtcatattc
     2521 gattgtcaac ttgatgtttc tcatatttta gtggctcctc tgtcttccat ttggtcttct
     2581 ctctaccttt ttaagattgt agcacaaaat atagctcctt tcttctctaa aacagaagct
     2641 atacaagaaa actgtggaaa tatatatttc agaaaaacaa aaaacagcca ccggatgaag
     2701 tatccgctga ctgttcaatt tgacggctca agttttactg tttgagatgc aagatactca
     2761 accaggcaat gggcacataa ttaaagaaaa aactgattct ttggcaaaaa cctattttat
     2821 tcagtaatgg ccaagaattt atagaaggaa ttcttgccag tccataaatc gccgcaaaaa
     2881 gcaaactgat cacagacagt ccaaggtata agtggcttac gtccatttgc ccattttttc
     2941 gatagatcaa gaaaagaaga actggaaaca ataaaaaacc actgtaacct aaacctgatc
     3001 cgatattatg gacccatgtc gagacattcc acgaagattc atgggtattg atactgaaca
     3061 aacctgtaaa gatacaatca cctataccat agaacgcgat ggctgctgta actagggtag
     3121 ctaacacacg agatgtactt acaaatgcag catacaatgc aggcaatgac aggatgaaaa
     3181 agatccctga aaccacagac caaataagaa acgcttttct tacaggactg gaaacctctc
     3241 caaatacact gatcacgctc ttcatttgat ctaattcagg ataatacaat cctaatacgt
     3301 acggagtcaa aaaatcactc agcacgccta ataataaaaa gaaaaaacca aatttacgta
     3361 aaaaatccat gatacgcctc caataacaat tacctatgct tttaattgtt attataagcg
     3421 actttatctt gatttaacag aaacctctat ttacacgaac atctatataa ctcattaaaa
     3481 actcgaatca ttttaaaaat actaaatatc taacgttcaa cgttcactta atccatctaa
     3541 acaatctttc aatttgattt tttccccact tcttttacgc gcgcctagct ctcctattgc
     3601 agtaaacaac agatcacttg acgaatttaa ggcagtttcc attgaatcct ggataacacc
     3661 gataataaat ccaataccga cgatttgcat cgcgacatca ttagagatac caaacaaact
     3721 acatgctaat ggaatcaata acagtgaacc gcccgcaaca cctgaagctc cacaagcagc
     3781 taaagcagac aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga
     3841 atgtacagca gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt
     3901 agcacctaac gggatcgata tggaataaga ctcttttgtc aatttcaatc tttcagccaa
     3961 catcatgttt atcgggatat ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct
     4021 ttctttcaaa caaaagaaaa tcaatggata gggattttct ttaataaaca aaaacgtgag
     4081 gaatgggtac acaactaatg cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg
     4141 cagatactct gccaatccag ctattccagt cgtagcaatt gattgataaa ccaatcctaa
     4201 aatccctatc ggagcaaatg caataatcac ttgtacgact tgtgacaacg ctatagatat
     4261 ctgatccacc aactcttttg tcgattctga acgtgctcga aaagctagtc ccaacaatac
     4321 agcccagaat aagacgccaa ggtaattcgc atcaataaga gattgaacag gattttgtgt
     4381 gacattcgtc aacatagtag ataaaacact ttccaaactt tgcggtgctt tttcagatac
     4441 tgtttcttgt aaagtaatag gaacagtaaa aattgaactt acaataactg ctacaagtgc
     4501 agaaagcaat gttcccacta aatataagat caaaataggc ttcacgaatg tcttattccc
     4561 aattttatgc ttggcaattg acgccatcgt caagaaaaaa accagaatag gtgcgattgc
     4621 cttcaagcaa ctgacaaaca acgttcccaa tattgaaata aatgtccatg tggggaaaaa
     4681 gataccaaac aatattccga ctaaaatacc aacaacaata cgttgaacta acgacatatc
     4741 caaaaacttc tctaacaaat gaccaccctt tcacaatttt aacaatcatt gtatcatttt
     4801 atgcaaagaa actaaaggta taattttcta aaaattctaa caatacaaac aaccatttct
     4861 tgctaatttt gtactttttc tgtctacacg ttgttttttg agatattcta agttaggatg
     4921 ttattaatgg gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga
     4981 ggtgtttatt cttgaaaaaa ataaagaagg agagttatgg cttcctgaca atctttgtcg
     5041 cttgtataat gatggtttta cagcgtatat tgatttcagc agtcactatt cctacatatg
     5101 caacaaaccc tggtcgattc tacatctata catttttaca aacttccgtt gttattggtg
     5161 caaatttgat cccactttac atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt
     5221 tacattattt aagtcgtttt gcctttatat tcctaatagc cacactagtt tgcaatatct
     5281 tttttttacg tacaagctgg cagcttgaat attcgtgatt attggcttat cctaacacct
     5341 atcagtcaga attactttac ttatgctgtt tcttgtactt tgcttttttg tagtattcct
     5401 tatacagttg gatttttaaa taaattatcg aaaacaacaa ttaaatatct actattattt
     5461 ttaacaattg gattagttgg ctgctctact ctatttaata aagacccgtt tgcattacag
     5521 aatggacaga gtattttatg gattttttat ttatttttaa tgggttatgg tttgaaagaa
     5581 tggaactgga aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt
     5641 ttattcggtt tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat
     5701 acagcgctga ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg
     5761 tttttatttt tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt
     5821 gctgtatttt tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta
     5881 tctacattta tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg
     5941 cagtttttta tttgctattt attagcagtg tccctcctag ttatcttttt attttatata
     6001 caaaaaatcc gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa
     6061 ttaactcagc gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta
     6121 gttgcagcat ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa
     6181 ggttggaaag aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg
     6241 atcctgacaa tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt
     6301 ttttggtacg ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta
     6361 atcaaaatgg aaatgcggga agagcctgtc tttccttcag atttaaaaat actgaccgga
     6421 ttgtcagaac tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt
     6481 ctcctattat tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg
     6541 aagattcatt ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt
     6601 gtctttttca ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta
     6661 aacaagactt tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc
     6721 ctaaacaata tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt
     6781 aatggaataa tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac
     6841 tggttaaaca atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt
     6901 gtccctaatc tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa
     6961 ggaacaggag gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg
     7021 gaaggactga caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc
     7081 cagctagtgg agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata
     7141 gccatccatc cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt
     7201 tttgacaggt tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa
     7261 caaagcccac gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc
     7321 aatacagaaa cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat
     7381 gattactata gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga
     7441 aaagaattac aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc
     7501 atccaagaaa tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta
     7561 cccgcaatct atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat
     7621 tatttcattt ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt
     7681 gttgctccta ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct
     7741 tactatgcgt tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca
     7801 gaaagcttgt ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt
     7861 atccaagaaa aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata
     7921 caatatgatc ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaa
//
LOCUS       DAZKAY010000111         7905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000111 DAZKAY010000000
VERSION     DAZKAY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.115"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..863
                     /locus_tag="ACG8WP_002457"
     CDS             162..863
                     /locus_tag="ACG8WP_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9028724.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1472..2488)
                     /gene="tsaD"
                     /locus_tag="ACG8WP_002458"
     CDS             complement(1472..2488)
                     /gene="tsaD"
                     /locus_tag="ACG8WP_002458"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9028725.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2485..2949)
                     /gene="rimI"
                     /locus_tag="ACG8WP_002459"
     CDS             complement(2485..2949)
                     /gene="rimI"
                     /locus_tag="ACG8WP_002459"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9028726.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2956..3498)
                     /gene="rimI"
                     /locus_tag="ACG8WP_002460"
     CDS             complement(2956..3498)
                     /gene="rimI"
                     /locus_tag="ACG8WP_002460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9028727.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3482..4204)
                     /gene="tsaB"
                     /locus_tag="ACG8WP_002461"
     CDS             complement(3482..4204)
                     /gene="tsaB"
                     /locus_tag="ACG8WP_002461"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9028728.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4395..5375)
                     /locus_tag="ACG8WP_002462"
     CDS             complement(4395..5375)
                     /locus_tag="ACG8WP_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9028729.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5399..6883)
                     /gene="galT"
                     /locus_tag="ACG8WP_002463"
     CDS             complement(5399..6883)
                     /gene="galT"
                     /locus_tag="ACG8WP_002463"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9028730.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6895..7884)
                     /gene="galE"
                     /locus_tag="ACG8WP_002464"
     CDS             complement(6895..7884)
                     /gene="galE"
                     /locus_tag="ACG8WP_002464"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9028731.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2221 a         1702 c         1394 g         2588 t
ORIGIN      
        1 tcaaaacaaa ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta
       61 aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa
      121 gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg
      181 attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt
      241 tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg
      301 acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac
      361 gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc
      421 ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata
      481 ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac
      541 agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc
      601 aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag
      661 aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa
      721 aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac
      781 tacgtgattc cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta
      841 ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaatatca
      901 gggtcactgc ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg
      961 gacaagactc aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa
     1021 aatactgtta gattaactag atgaagcgtc ttgtcctcat ccgatctaga ttattcgata
     1081 ttcctcgaca ataaacgatt actcagtgag ggcaatcaga ggaacggata atgataaata
     1141 agctgtaaaa aatagcaaaa taacaataat gattaaaagt atgtaaaaaa tagggctttg
     1201 acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc
     1261 acaagtcata atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca
     1321 gagttaaacg acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg
     1381 gtaataagaa cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta
     1441 cttgatgcag ggcagctttt tcacaacctc tttattcaga taaaaccaag cttggatcag
     1501 cattcaactg atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa
     1561 tcattgcagc attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga
     1621 gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc
     1681 cagctataat cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca
     1741 atacatcgat cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc
     1801 cacgctgttg tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga
     1861 aatcataatt atcttcatgg atcatcgctc gcggaaaatg gtaattatct tttccctgat
     1921 gagctagctg atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt
     1981 tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat
     2041 cttcttgcat atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa
     2101 actggaatgg tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa
     2161 taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg
     2221 agccgacaag tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac
     2281 cagcttctac cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag
     2341 ctacttcagg tactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt
     2401 tagataagat ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt
     2461 caatcgccag aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat
     2521 aatgatcgca tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa
     2581 cttttctgat tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac
     2641 atgcagcgaa tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc
     2701 ttttccttgt tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt
     2761 cgagtatcca ataaagccac agatctggtt atcttcagct aaaagcagat aatcagagtg
     2821 aggctgttgg atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc
     2881 attttcgcta atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct
     2941 gcaaatcaac taatctcact cattttctaa aagttttatc atatccaacg catcttcttt
     3001 tgtttcagtg taatatcttt ttttgatcgt tgttgcttga aaccccaatt tccgataaag
     3061 tctttgtgca tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat
     3121 tatagcaaag ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc
     3181 cggtcgaacg gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa
     3241 accaatcatt cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa
     3301 ttctgatata aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac
     3361 atccagcagc tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat
     3421 ttgttcgact gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc
     3481 tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt
     3541 ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcaa
     3601 ctaaagcacc taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat
     3661 taatcatcgc actaggcaat atttccttta tttcagagaa gaatttgtga caatcactcc
     3721 cgacgaaata aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa
     3781 tatgtacatc ttcaagtaca gtttctaatc tattttcttt ccagcggtat gccccagcgt
     3841 atacattttt tcttcgagca tcaaacatgg gcacaataat attatccaca ccgatgcaat
     3901 tagccgcgat ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa
     3961 tcgtttttgc tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa
     4021 ccgcgattcg gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg
     4081 caggcatcag tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt
     4141 gttcgtcttc taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa
     4201 tcatatattt gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta
     4261 ttctgaggat gaaaatacca aaggcaaaac tagtacaata atacatgatt atctgctggc
     4321 gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg gtatcactcc attttcccac
     4381 agcttttcca tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc
     4441 tttcttgaaa ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct
     4501 cccatccatt cggtataaac ttttacagta gaaagcgagg gcgttacata tttcgctaca
     4561 ctgacatcat tgaagcccat cacagaaata tcttctggta ccactaaacc tttttcttga
     4621 atcgctttca gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca
     4681 tgcggattcg tttttaaata ttgcttcact gcttgatatc ctgactcgac agaaaaatct
     4741 gcttcgataa ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata
     4801 attctgggat cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata
     4861 gcgatatgtt gcgctttatg ggagacaaag tgttcaatga ctttttgaac acttgactta
     4921 aagtcgacaa ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag
     4981 gcattttggg atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc
     5041 gttccatctg ccggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt
     5101 gcttcttctg cctttttttc gattcctagg cgaatagata aataataaag atcttcaagc
     5161 tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt
     5221 ttacgatgtt tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct
     5281 aagccaacgg aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact
     5341 cctgccaact gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt
     5401 ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc
     5461 cagcgtcttc cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga
     5521 ccgtatcttt cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat
     5581 attcagccat ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt
     5641 tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt
     5701 tgatatgttg gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat
     5761 tgttgtctcg aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg
     5821 taatcgtatg gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg
     5881 aatatgcttt ccatttttcc aatactgagt tagctgcttc aataagcaag tttttctctg
     5941 ttgattgcaa acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat
     6001 aatcttctag tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc
     6061 ctgcctgata atgatcatga gaaagaatcg atcctccaac aattggtaag tcagcattcg
     6121 aaccaacaaa gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct
     6181 ttgtgatttt catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag
     6241 cataagggga atattggaat ccccagcttt caccgtctag attcattcga ataatccggt
     6301 gattggtgcg ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca
     6361 tgcaggtagg atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt
     6421 tttctggctt agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa
     6481 atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa
     6541 agaaatactc agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga
     6601 cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg
     6661 ctggcaattc tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt
     6721 ctacagattc ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca
     6781 tccctaaaag acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga
     6841 tcgcaagtgt ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc
     6901 ttcataacca tttggatggc tgacatgcca atcccatgct gttttgataa tcgctttgat
     6961 atctgtgtat tcaggtttcc agcccagaat ctctctcgct ttttcacttg aagcgactaa
     7021 tcggctagga tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac
     7081 ttctctagct gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa
     7141 gaagttgctt tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa
     7201 atcttctact tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc
     7261 ataaacagcc aaggcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg
     7321 cgtttctggt gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa
     7381 gtagcgcaaa gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc
     7441 catcatcaat ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat
     7501 tggagactct ttcggctctc cataagttgc tgcagtagaa gagaatacga ttttattcac
     7561 gttgaactcg tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata
     7621 catcaatggt ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc
     7681 ttcaatcggt tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc
     7741 ataaaaatga gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac
     7801 agctacttga tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc
     7861 tccgcctagt actaaaattg tcataaaaaa cactcctttg attag
//
LOCUS       DAZKAY010000112         7797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000112 DAZKAY010000000
VERSION     DAZKAY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.116"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8WP_002465"
     CDS             complement(105..629)
                     /locus_tag="ACG8WP_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9028732.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8WP_002466"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8WP_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9028733.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8WP_002467"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8WP_002467"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9028734.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8WP_002468"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8WP_002468"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9028735.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8WP_002469"
     CDS             3157..4026
                     /locus_tag="ACG8WP_002469"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9028736.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8WP_002470"
     CDS             4052..4567
                     /locus_tag="ACG8WP_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9028737.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8WP_002471"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8WP_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028738.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8WP_002472"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8WP_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028739.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8WP_002473"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8WP_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028740.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8WP_002474"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8WP_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9028741.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8WP_002475"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8WP_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9028742.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2232 a         1614 c         1382 g         2569 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaa
//
LOCUS       DAZKAY010000113         7710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000113 DAZKAY010000000
VERSION     DAZKAY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.117"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      4..170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            318..875
                     /locus_tag="ACG8WP_002476"
                     /pseudo
     CDS             318..875
                     /locus_tag="ACG8WP_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            933..2972
                     /locus_tag="ACG8WP_002477"
     CDS             933..2972
                     /locus_tag="ACG8WP_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9028743.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3081..4232)
                     /locus_tag="ACG8WP_002478"
     CDS             complement(3081..4232)
                     /locus_tag="ACG8WP_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9028744.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4386..4916)
                     /locus_tag="ACG8WP_002479"
     CDS             complement(4386..4916)
                     /locus_tag="ACG8WP_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9028745.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5015..5365)
                     /locus_tag="ACG8WP_002480"
     CDS             complement(5015..5365)
                     /locus_tag="ACG8WP_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9028746.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5522..7051)
                     /gene="ahpF"
                     /locus_tag="ACG8WP_002481"
     CDS             complement(5522..7051)
                     /gene="ahpF"
                     /locus_tag="ACG8WP_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9028747.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7061..7624)
                     /gene="ahpC"
                     /locus_tag="ACG8WP_002482"
     CDS             complement(7061..7624)
                     /gene="ahpC"
                     /locus_tag="ACG8WP_002482"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9028748.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2630 a         1479 c         1415 g         2186 t
ORIGIN      
        1 tagtaataac cttgttaggt gaggctccta tacaaacata ggctgttgcc cagaaacgtc
       61 gaaagtcgcc aatgggtaaa acagaaactg ttggattaag gctttttcca atagctaaga
      121 atgttttctt tacgttgtat agtgctaaaa ctcggacgat gagatgatgc taggtcagaa
      181 ttcttgttcg aaaaggtttc tgaccttttc tttatatttt tgtgactgac ttatttgatg
      241 agacaaactc gctcctcacc tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa
      301 aaagatcgat tggtggaggt cgctcggatt cacttctggt tgcacaatac aaccagaaaa
      361 cacaacaagc aaaactgatc tctatcatga gagattcata tgtcgatatt ccaggttacg
      421 gaatgaataa gataaatgca gcttactctt atgggggagt tgatttatta aatcaaacat
      481 taaaggaaaa tttcaaattt gaagctccct attatgcaag tatcacattt caagatttta
      541 tcgattgcgt taatgaactg tttcctggtg gagtcaagat cgatgcagaa aaatctctag
      601 atttagatgg cgtatatata aacaaaggag agcaagtgat ggatggtaat actttactcc
      661 agtatgctcg atttcgggaa gacaaagaag gagacttcgg gaggatcaga aggcagcagc
      721 aagtgatcaa agctatctct cagcagctga aagacgtaac ttcaatattc aagctaccca
      781 aagcggttgg gaaattactt ggtagtatcc aaacgaacct tccagaaagt gtgcttctgg
      841 attgtggtat ggatttcctc aaagacatat gttagaataa acaggtataa atagtgaaat
      901 aaaggaatga caagcaagag aaggaggaaa aaatgaaaag aagtgacaag cacggcaaaa
      961 atcgaacagg cgcttatatt gccggcgcag tgatcttaat agcagctgcg ggtggcggtt
     1021 atttttacta ccagcactat caagaaaccc aagcagtaga agctggagaa aagacagttg
     1081 agcaatttgt ccaagctttg aacaagggag attataacaa agctgcagaa atgacatcaa
     1141 aaaaggcggc aaataaaagt gcattatctg aaaaagagat cttagataaa taccagaata
     1201 tatacggtgc tgctgatgtc aaaggacttc agatatcgaa tctaaaagta gataaaaaag
     1261 acgattctac ttacagtttt tcatataaag caaagatgaa tacctcatta ggtgaattga
     1321 aagatctttc ttataaagga acattagaca gaaatgatgg tcaaaccacg atcaactggc
     1381 aacccaactt ggtttttcca gaaatggaag gaaatgacaa agtaagcctg accacacaag
     1441 aagcagcaag agggaatatt atagatcgaa atggtgaacc attagcaaca acaggcaaac
     1501 taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg aggcgaaaaa acagccaata
     1561 tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc tatcaatcag gcgatttcac
     1621 aaagctgggt acaacccgat tactttgtcc cattgaaaat cattgatgga gcaacgccag
     1681 aacttccagc tggagctacc attcaagaag tagacggcag atattatcct ttgggtgaag
     1741 cagctgctca gctgattggt tacgtgggag atatcacagc agaagatatt gataagaatc
     1801 cagaattaag cagtaatgga aaaatcggtc gctcaggttt ggagatggct tttgataagg
     1861 atcttcgtgg gacaacaggt gggaaattaa gcattacaga tgcagacggt gtagagaaaa
     1921 aagttctgat cgaacatgaa gttcaaaatg gcaaagatat caaattgaca atcgatgcga
     1981 aggcacaaaa aatagctttt gacagtctag gaggaaaagc tgcatcaact gttgcgacaa
     2041 cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc aagctatgat ccaaacaaaa
     2101 tgacaaacgg gatctcacaa gaagattaca aagcttatga agaaaatcct gaacatccat
     2161 tcatcagccg atttgcgaca ggttatgctc ctggatctac gtttaaaatg attacagcag
     2221 caatcggtct cgacaacggc actatcgatc caaatgaagt gttgacgatc aacgggctta
     2281 aatggcaaaa agacagttct tggggatctt ttaaaacaac gcgtgtcagt agtgatgtat
     2341 cacaagtaga cttaaaaact gctttgatct attccgataa tatatatgcg gcacaagaaa
     2401 cgttgaaaat gggtgagaaa aaatttcgta caggcttaga taaattcatt tttggtgaag
     2461 accttgattt gccaattagt atgaatccag cacaaatttc taatgaagat agctttaact
     2521 cagatatctt gctagctgat actggatatg gacagggcga acttctaatt actcctatcc
     2581 agcaagcagc aatgtattct gtttttatca acaatggcac acttgtctat cctaaattga
     2641 ttgcagataa agagacaaaa gataagaaga atgtcatcag cgaaacagca gtacaaacga
     2701 tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg tacagcacat tctctttctg
     2761 ctttagggat tccgttggca gcgaaaactg gtacagcgga aatcaaagaa aaacaggatg
     2821 taaaggggca agagaacagt ttcttgtttg ctttcaatct tgataaccaa ggttatatga
     2881 tggtcagcat gttggaaaat aaagaagatg atgattcagc aaccaaacga gcatcggaac
     2941 tattgcaata cctcaaccaa aattatcaat aaaaaattgc tttttaagta cagtaaaagg
     3001 actgtggcgt tatcgtcaca gtcctttttt caaataaacg gttttcacga tgtctctttc
     3061 acaacttctt tttgatttac tcacacttca taaacttttt ttgtaaaata tttgagaaca
     3121 agtggagaaa taagtgtact taataaaatc acgataacta aaggtgaata gtactgtggt
     3181 tcgatcaatt gactttgctg tccaatttgt aaaatgatca aagccatttc tccacgcgaa
     3241 atcataccag caccgaccat caatgaacta tgttgagaaa aaccggcaat ctgagcacca
     3301 aaataacctc caagcaattt ggtcaaaatc gctacaagcg tcaatataga aataaagagt
     3361 agttgttcac taaagtcaga aaaatcaact tctaatccta cactgacaaa aaatactggt
     3421 ataaatactg catatcctaa agcctctaca ttgtggtata cttcttcttt aacctttgtc
     3481 tgactgacag ctatcccagc aaaaaaagca ccaatgacag agctaagccc aacaagatcc
     3541 gctacgtaag acattcctaa acaaataact agcgatacga tgataataga agaatttgcg
     3601 tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc atttgactaa taagaaaata
     3661 aaaacaaaat aaattagctg ttcaatcaaa acaagcggta aagaagcatt gctagaagct
     3721 tcccccgtta gaaaagatag gctgaaactt agaactaaaa cgactaaaat gtcatctacc
     3781 acagaagcac ccaaaattgt tgatccttct tttgtattca ctacgtttag ttcttttaat
     3841 acttctactg aaatactgac agaagttgcc gcaagaataa tcccaaagaa aatcgcttca
     3901 tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa aaacaaccgg gaagatgatc
     3961 ccaagcaaag caacgaacat accaggtcgg aaatattttt tcaaaagaga taaatcactt
     4021 tccagaccag ctaaaaacat taataagatc acaccaatct ccgaaaagtc atggactaga
     4081 atatcaggtt tgacccaatt caatccagct tggcctagca aaacaccaac aagtaattgt
     4141 ccaatcactg ctgggatacc taatctccgc gagatatgtg agccaatcgt tgttgaaatc
     4201 aatatcaggc ataaaatacc aataaattcc attctcatca ttcctttgta taaaatatag
     4261 cccgaactta gcataacata cattattgaa aaaataggaa aatatactca caaaaaaacc
     4321 aacaatccaa aagatttttt tggattgttg gtttttatac ggtctatttt aataactaaa
     4381 aatgattaac gtatagtcat ttttcttatt ctgatgaaat tgataatctc ctggatgccc
     4441 atgaaaatca aaataaagcc gaatatttgg aatagaagca tcaaactttt gaatggattg
     4501 aacaataaga cgaatccgcc aatcaacaac agaatactac tataaacact ccaactttta
     4561 gcttttgtat tcatgctgaa gaacaactgg aacaagctgt tgatgataac agctaatcca
     4621 agaagaatcg gcaggataga aacgattgca ggtgcaaaag catagacgat cagagctaag
     4681 atcaggaaaa aaagaccact gaacagaccg atactccaag aacctgtttt ccttttaaaa
     4741 cggtaagcct caaaaagatt caacaaccca agcaaaacaa gataggcggt gatcagataa
     4801 ccgataaaat gaaaaacagc agaagggttt agcacaatcg caatcccaga aataatataa
     4861 acagcagctc ttaataaggc gtatttttga atattttgtt taatcgtctc aaacatataa
     4921 gaaaacctcc tttgacttat tttaacgtga actagtcatt ttaccaaatt aaatgttaca
     4981 aaatgagcga aggagaagaa tatctctgga aaaatcaact tctgaggatc ttttccattt
     5041 ttttgccttt tgctagctca tcgatcattt tatctaaata acgaatttct ttcatcaagt
     5101 tatcctcgat gtcttctact cgtacaccac aaacaacacc tgtaatcaag gaacgtgaag
     5161 gattggggac aggggcttcc gcaaaaaatt gttcgaaagt cacttgtttt tgtagttgat
     5221 attctagttc tgtttgggaa taacccgtca gccaacaaat gatttcatct acttcctctt
     5281 ttatacgatc ttttctctcc gcttttgcta catacatagg ataaacgctt gcaaaacttg
     5341 tcgtaaaaat acgattttgc gccataatca gtcacctttc tttttttcaa attatttact
     5401 acttatagta tataagaaaa agagaagaaa aagcgattat tcaaaaaaac aaaaaggatt
     5461 gtgacaaaat tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag
     5521 ctcaagtgcg catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg
     5581 aaacaatgat ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga
     5641 tattcgttgc gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc
     5701 tatctagcca atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta
     5761 gtgtatgcat ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac
     5821 cgctgatttc tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt
     5881 tatcctgtaa gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt
     5941 ttgctaaccc agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag
     6001 caatctcttt tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat
     6061 ttttaaactc tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga
     6121 tggcggtttt tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct
     6181 ctttatttaa ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat
     6241 gactttctac ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt
     6301 caatccctag tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga
     6361 ttcctttacg tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta
     6421 atacatcata aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt
     6481 caacaatttg ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag
     6541 ttggcactgc catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca
     6601 tcgtatgagt aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg
     6661 ggcaattatg acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt
     6721 tgattttaag actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag
     6781 ccagtaaaaa agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt
     6841 ctttgccaac tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag
     6901 agattttagg agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt
     6961 cactattatc caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt
     7021 cgagattttg tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat
     7081 caaaacttgg tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg
     7141 ggtgggtacg tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac
     7201 cgattccatc tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg
     7261 ttcctcgctg agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat
     7321 gagacggatc accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc
     7381 atgctttgtg agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt
     7441 tcaatgtact gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat
     7501 cagctggata gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta
     7561 aaaattctcc ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg
     7621 acatagtaaa acctccaata ataattattt atttataatt attgtaattt agttggagtt
     7681 gcaaagtcaa tggatatttt gttatttaat
//
LOCUS       DAZKAY010000114         7267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000114 DAZKAY010000000
VERSION     DAZKAY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.118"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..422
                     /locus_tag="ACG8WP_002483"
     CDS             <1..422
                     /locus_tag="ACG8WP_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9028749.1"
                     /translation="DLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASN
                     SDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRR
                     AKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            498..716
                     /locus_tag="ACG8WP_002484"
     CDS             498..716
                     /locus_tag="ACG8WP_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028750.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            703..3381
                     /locus_tag="ACG8WP_002485"
     CDS             703..3381
                     /locus_tag="ACG8WP_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9028751.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3372..3569
                     /locus_tag="ACG8WP_002486"
     CDS             3372..3569
                     /locus_tag="ACG8WP_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028752.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3573..4184
                     /locus_tag="ACG8WP_002487"
     CDS             3573..4184
                     /locus_tag="ACG8WP_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP9028753.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4169..5560
                     /locus_tag="ACG8WP_002488"
     CDS             4169..5560
                     /locus_tag="ACG8WP_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9028754.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5776..6090
                     /locus_tag="ACG8WP_002489"
     CDS             5776..6090
                     /locus_tag="ACG8WP_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028755.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6083..6577
                     /locus_tag="ACG8WP_002490"
     CDS             6083..6577
                     /locus_tag="ACG8WP_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028756.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6645..6959
                     /locus_tag="ACG8WP_002491"
     CDS             6645..6959
                     /locus_tag="ACG8WP_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028757.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7058..>7267
                     /locus_tag="ACG8WP_002492"
     CDS             7058..>7267
                     /locus_tag="ACG8WP_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9028758.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2776 a          986 c         1411 g         2094 t
ORIGIN      
        1 atgatcttct tgaatcactt tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa
       61 catttattga agaaaatctt cttaaacaaa tatggggaga aagtattgtg aattgcttaa
      121 aactggctag caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa ttcaaatatg
      181 cgattagatc tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat gaaatttgta
      241 ataacaagta ttttgtagat catgttagag aacagctatc aatgacttta cgtgacttga
      301 tacgaagagc aaaaacagat catcgtatca aacaaaaaga cgattatatc tttgcttctc
      361 taaaaaataa agcgttagaa ctaattgaat tgcaaatcag cgaaggaata gataagcaat
      421 aagaatagaa ttgagtcaaa tgaaatggag gggtatctcc acttcatttg agaactttga
      481 atataggagg ggttattttg attaaaaatc gtttaataga tcaagtttgg tgtctagatc
      541 aaaatacaga tagtctcgaa ttaatcgaga ctatttgctt caacactata gttctcgatt
      601 taagagctga aaatgataat tgtgtaactc atgtcataat taatcaaaaa atggctcaga
      661 agctctctga gagcctcaga aaatgggcta agggaggtaa cgatgaaaat aattgaaaaa
      721 aagattgcca ataaaaaaat aaattggttg gttgttaatt ttcttgttct gatagctatg
      781 attattttta caagtaatat tgctcatgcg agtattttca attggtttga tgacacaaaa
      841 agtcaaacag agtttttgaa taattctctt tatagacctt acctaaaaaa gtacaatgga
      901 gatttcctta gtcagttcgg gatttggctc ggatgggctg tggtaaaagg catgtttact
      961 gtgacagatt cgattcaaaa tatgattcca gatgtgctgg atttgttcaa tttcattgaa
     1021 agtacagggc taaataatgt ttatcaatca gccgtaaata caattgtcat tggattaatg
     1081 atcctttcat tgatgtttgt tggttacaaa atgattactg gaaaaggaac agtggattta
     1141 aaatcggttg gaatgaacgt agtgatgtct atagcattaa tacttttaat gccaaccatg
     1201 atttctagtg gtattcaatt tgctaaaaca ttttataagg actctactac gattacaaat
     1261 tcagatgacg gagtcgcttg gtcattgata aaacaaggag taacagattt agcatatatt
     1321 aataaaacag atcagtactc aagcattgat aaaacggagg atcgaaataa tttaacacga
     1381 aaaaattttc aacagacgga tctcacacaa gtgttaacgg ataaagtgat tgataaattg
     1441 gaaaaagaaa atccaattgc ggacaatctt cgttatgaat tagttgaaaa ttctaataat
     1501 gaatttgttg ctacaaagtt ttctgacaac ttcttatcaa cattttctga tagcttgaaa
     1561 tcaggctatt accgatacca agcaaatcta tgggggattt caattgggtt gacagcgtta
     1621 gcaatcgcct atgtgttcag cgcgtttgtg atcattacag caatactaga acttgcattt
     1681 aaacgtgtgt taggtgttct tgtttttgca acagatattg agacaggaca gcgttcaaaa
     1741 gttgttctat cagatattct acaatgttac ttaactgttg ggtttcaagg atttgggtta
     1801 tcaatgtttg ctatgtttat caattttctg aactcaggac aaggtatctc aacaaatatt
     1861 tttataaaaa caattgctta tatctgtgcc gtttttgttt taatcaaagg cagtggaaca
     1921 gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct
     1981 gcttttggaa tgggcgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa
     2041 ggaagcggag aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa
     2101 aaagctggca aaactggaag agctttggga tatgcccatg aacgtggatt atccggttta
     2161 gcttcagatg gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt
     2221 gatatagcta atgataccaa gaataagttt aaagaaggtt tagatgatgg tacagtgtcc
     2281 gctattaata aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa
     2341 tatgctgatt ccgtgccaac acgtctttca gatagaaaag atggtgctaa agttgaaaat
     2401 gccgatagaa ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa
     2461 tcgaataata atcctgcaag cgcaattcag caaaaagtcc aacaagatat tgaagaacgt
     2521 aaaaatgcta tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgtcat
     2581 gaagcgaaat acacgcctga agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta
     2641 gaagggcgga caggcgctaa tattgacgga aaagaagcgt tgaccaaaga aagaattcaa
     2701 gaagctaaaa attcaaatac aggattagaa aaattagtca aagaaaatcc acaaaattct
     2761 acaagtagtc atggtggccg atcagtagat gtccgagaaa atattcaagg ttcactgaat
     2821 ggtcatgttg gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatgctcaa
     2881 ggtactaaga caattgatgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa
     2941 acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg
     3001 gatgtccgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga
     3061 gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac
     3121 attcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac
     3181 agaaagtcag cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc
     3241 cgtgaaaatg ataagggatc aaaatcaaga cgaagattca cttatgaaga caatgagtta
     3301 ttcagagata cacttaatga tcctaatcca cttttcgata atttattaag aaataaaagg
     3361 gggggattgt catgcaatta attctaaaga ttagtacgca tgatttctta gaacttgacg
     3421 aattatgtga atcagtaaag aacgcaacaa atgaaaaaat cacggtcact aattatattg
     3481 aactgatgat tcaagaagaa ttaaagatga aaaatgaaat agagagcaat ttcaaatgtt
     3541 gctctctaga aaaaaatgtg aggggataat caatggatga taaacaaaaa atgtacgaaa
     3601 aaaatcgcaa acatagtgag gccttaaatt ttcgtgtcga taaaaagact tatcttgaac
     3661 tgacattgat tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt gagcggttaa
     3721 agggactcaa atatgaaccg ccgttgttag ctgaagatga tagggtagtt ttaaccaaac
     3781 agttaaatca aattggtaac aatttgaatc aagcggtaaa ggcattaaat atcattgcta
     3841 aatattttag agaagaatct gaagataaaa aagaaaagag cctacatttt agttttgatg
     3901 ctagccgtta caacattttt aaagatgaac ttgcgaatag catagcaaaa aagttgatga
     3961 ttagttatcc agatgacatt tttttggagt ccaatcggag tgccccctct ttttttaaat
     4021 tatcagaagt agaaaaagaa tatttcgaat attggataga aaagcaaact agaccgggta
     4081 cgaaagaaga gtattcccaa aaagttgatc ggaatttaat tcactcaatt cagattattg
     4141 caagtttaaa agatgaggtg aaacaacttt gggagctact ataacatcga aaggacgcaa
     4201 gaaaaattta tcctcgctat tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt
     4261 tgcagtctca ggaataaatt gtagtgatga tagtgaggaa gcactgcaag aaatgaattt
     4321 agtacgtacc aagatgaata aatctaaaga tatagcacaa gcgtttcatg ttattcattc
     4381 gtttgataag agtacttcta aagagttaag tattgataaa atgcacgaaa tagctgtcga
     4441 atttgcagag aaagcttttc caaattcgca aatcattgtt gcaagtcaca atgataaaga
     4501 tcattttcat agtcatcttg tcattaataa tattaacatg gaaacatgta aaaggttgcg
     4561 tattgaccct aaagatttag aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca
     4621 tggtcttgaa tcagtgaata atgagtacta tgaaaactta gaaaagagaa ccaatcttta
     4681 ttcaaacgat ccttctaaga tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa
     4741 tgcgatcgaa gcaacgttgg aaaaatgcga agtgaattct tttgaagatt tttcggatca
     4801 tttggcaaaa gaatataaca ttgaagttta tcaattttca aaaacaaata acaaacttgg
     4861 ctatgttctt tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt taattacttt
     4921 aggaagagat aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc
     4981 aagaaagtta gggaaaagat attcatttga atctgtttct gaacaacttg aaaagaattt
     5041 agaagaatca catataaaaa atttttttaa tatggaaaga gagcaagtta tgtcgctaat
     5101 tagtggagta aattacaaaa ctggtgagtt aaatgtaccg tttgtggaat tagattccat
     5161 cgcacaaaag caatttagcg accgtgaagg cgatgaaaaa ttcattaaat atgatgactt
     5221 cttgcgagag gtagacaagc attttgtaaa tacaatgtct ttagaagatg atcaagaata
     5281 cacgattaaa cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac
     5341 aatttctatt aaaaagactg atcagaaaga gttatttcta ccggcaaagg gttttgaaaa
     5401 ttgttctcat acaccttctg ctaaacagat tcactatcaa atcagtcgca taatgaatga
     5461 cgatgtaaaa atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa
     5521 tcagcaaaaa aggcaacaaa attttgaaat ggaattctga atgggcttgt tagtggactt
     5581 tccagttttt tgcctatgaa gagttgcttg gaaagaagca gtttttagga catacgccag
     5641 ctacccatct acttgccagt caatccgccc cgctcacgct aggctacttt ctggcaagtc
     5701 agatggcggg taaatctctg agggagcaag ctccctacag actccctccc aaaaaaagaa
     5761 aggtggaaga aaaaaatgaa ctatccatat tttaaagtct cggcttcaga agaaactaaa
     5821 gaaatattca acaattttta caaccaaaac aaaggaattt ttggttcaaa ggcaaatatg
     5881 tttcgtgtta tggtttcaaa tttaccagtg ttagctagtc catcaaataa taaatttaat
     5941 gatcctgaaa gcattaaatt tgaacaaaaa atttcagaac tagaatcaat gataagtaat
     6001 gaagtaattg aaaagctgga tgacattgat caaaagttat cgtattctct aaaaaacaaa
     6061 tacaaaacgg aggagaaaaa agatgtttaa agtttacttc ccgtttcagt ggctcgttaa
     6121 aggaattctt ttagtgctac taggattgat tctttttgga agttatctaa caattaaagc
     6181 cagacatttt ccagaagaaa agccaataaa aattgtaaca aaagatttaa aaataggtga
     6241 aacttatcag gcagatgggg ttgaactaac atttaaaaat attgaagtga ccgaaaaaag
     6301 aaatgaattt gcacaacatg atcgtgtatt acaagtgacc tatacactta taaatgatac
     6361 agaaaatccg atacgtccac aacaatggtc gattgaaaat aaggatggtg aagaacttta
     6421 tgagtatgat atgatattgg ataatccaga gtatattgaa agtggcaaag aagaaacagt
     6481 aaattactat tatgaattac cgattgaaag gcaggactac caaatcgtag tacgtagaga
     6541 gaatggttac atagttgggt tgccatttac tttataagtt ttattttgtt agtagttttt
     6601 aaggtaatat cagttttgtg atttttgaaa atggaactgg gagaatggct gaggaaaata
     6661 aagttgttcc gtttgaaaca ttagtgataa ataaggaaga gtttgagcaa ctttttcttg
     6721 atttttttat tcaatatgac atgatttatg attttagttc gtcactagat ttatttgaat
     6781 tagaagcaga aataatagaa aacagtcgag agaagaatgg taaaatcatt atttcagtag
     6841 aaaaattcaa tgaattattc tcacaaacat ttgaaataga tccagactat tatcaaccac
     6901 aagaagtttt tcgagagttt agaaataaga ttattggttt tagtcaaact acaaaatgaa
     6961 agcttaataa agcaagtgtt gatagaatta ttcctcaacg cttgctttat tttatgggca
     7021 cttcataata gaggtataga gaagagaggt attcattttg gatactatta ctccaacaaa
     7081 cttcaaagaa aatataggta aagtgttgca agaagtagtt cattcaaatt ctgaaataga
     7141 aattactagg gattctaaaa atggattgaa cgatggcgtc attatgattt ctaagagaaa
     7201 atgggagttg ttacaagaag aactttattt acaacgaacg ggtacatggg cttatgtatc
     7261 tgattta
//
LOCUS       DAZKAY010000115         7145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000115 DAZKAY010000000
VERSION     DAZKAY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.119"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACG8WP_002493"
     CDS             <1..613
                     /locus_tag="ACG8WP_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9028759.1"
                     /translation="GEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEE
                     EFGHLEGDTIVGIHHKSAIITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFANPGTPSQRGLNEHSNGLLRKNGLPKEMDF
                     NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(681..1454)
                     /locus_tag="ACG8WP_002494"
                     /pseudo
     CDS             complement(681..1454)
                     /locus_tag="ACG8WP_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1490..2677
                     /locus_tag="ACG8WP_002495"
     CDS             1490..2677
                     /locus_tag="ACG8WP_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFP9028760.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            3410..4378
                     /locus_tag="ACG8WP_002496"
     CDS             3410..4378
                     /locus_tag="ACG8WP_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9028761.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            4446..5204
                     /locus_tag="ACG8WP_002497"
     CDS             4446..5204
                     /locus_tag="ACG8WP_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9028762.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5272..5982
                     /locus_tag="ACG8WP_002498"
     CDS             5272..5982
                     /locus_tag="ACG8WP_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028763.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6018..>7145
                     /locus_tag="ACG8WP_002499"
     CDS             6018..>7145
                     /locus_tag="ACG8WP_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9028764.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2432 a         1115 c         1439 g         2159 t
ORIGIN      
        1 gggagaattc aatgttttac atttaccgat gcaaggaaaa cgaaaaccaa atggttataa
       61 ggaaaaacgt ggaaaacagg cattcaaaag aaatatttct gaacgtaaaa aagattatgt
      121 cgttttcgaa gaagaatttg gacatttaga gggtgatacg attgtcggca tccaccataa
      181 aagtgccatc atcacactcg ttgagcgact ttcaaaagcc attatcgcct tgaaaccaga
      241 aggccgtaag gcagttgata ttgaaaattc gattaatgaa tggcttcaat ccgtacccaa
      301 aaatcttttc aaatcaatta cctttgattg tggaaaagaa ttctctaatt ggaaaagtat
      361 tagtaatacg aatgatattg atatttattt cgcaaaccca ggaacgcctt cccaacgggg
      421 attaaatgaa cattcaaacg gactcctccg aaaaaatgga ctaccaaaag aaatggactt
      481 caaccaagtc aatcaaggat tcatctcatc cgttgcgtcc aaaagaaacc atatccctag
      541 aaaatcacta aattaccaaa caccattaga agtttttttg agttacgtaa atggaaagtt
      601 ttgtctcgct taatttgaca aataataagc caataaaact tagtccgata tgcggcgcgt
      661 tctgtgcgcc gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt
      721 gcccgaaagt tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg
      781 ttatacagcc cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc
      841 gattggtagc gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa
      901 attgttttaa gggtttcttg aaagcctacc gtatctcgtt taacgaaata atagcgtaat
      961 aattgacaaa aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcattaacg
     1021 atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtgcctcc
     1081 catgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc
     1141 tgattttcag agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag
     1201 ctgcgattga tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag
     1261 accgttttaa gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt
     1321 aaacgggcct ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct
     1381 tcaagggtcg taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca
     1441 gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt acaaaactat tgcaaaacac
     1501 agcgcataag aatccttaca aaacagtaga tgaagcaacc caattagatc ttgttatagt
     1561 tgttaatcag ctcttaacgg gttacgattc aaaatgggtc aacaccctat acttagataa
     1621 gtcacttgaa tatcagaaca taattcaggc tttttcacgc acaaatcgtc ttaatggtcc
     1681 tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca catacgatgg aacagaacat
     1741 tgaagaggct attgagttat actcaggcaa acaagcaaaa gggttatttg ttgaaaaatt
     1801 atataataac ttagaaaagt tgaacgatat attcgaagaa attaagtccg tatttaatat
     1861 ttctggcata gaaaacttca gtcatttacc ggaggttcca ggggaaatta agcaatttgt
     1921 aaatctttat aatcaactaa tccattgtta tgaatcagcg atgatacagg gattcacatg
     1981 ggcaaaatcg acttatgaaa tccaggatta tgaacctata acagtagtaa tcacagaata
     2041 tgaatttaat atcttggcac aacgttacag cgagatagag agagaaccta gagacggaaa
     2101 tgatagtata gcgtatgaca ttgattacca attacctgaa ggtagtcgta gtcgtattaa
     2161 ttatgagtat ttaaacacaa acttcaaaaa atacatgaat ataaaagaag aacgtgattc
     2221 gaatttaatt caatcattgt taaacgactt acatcgcaca tttgcgcaat taccagtgga
     2281 gttacagcca tatgctgaga tgattctaaa tgatgtaaat aatggggaat tagttatcaa
     2341 agaaggatgg gaatttactg attacctcaa tgaatatcag cttagtgaag aggacgattt
     2401 tatccaagat ttagtggatt caaccaatat taatgaggca ttattacgtg agatgttgga
     2461 tttacgctta accgaggtaa acattaatga atatagtcga tttgataaat taaaatcaag
     2521 tgttaacgta ggtcatttca gtggatatat cgagaaaaat ttaggtaaaa tggtaatacc
     2581 aatcaaggta aatatgctaa ttgacagatt attgcgtgca tttttattag aagatgtatt
     2641 cgatgttaca gagtacataa agaattactt taactagagt acacctacat cttatagtac
     2701 ataaaaaaat tcatcttttt tagtttaata attcaccaaa aaggtgagta atgccatttt
     2761 tgaaaattga gcttgtttta tgtctacatc tgtatccgta tataatccaa aggattataa
     2821 actcagaaga gtctttattt tttctgaata taactactgt atcaaaaaca atcgattatg
     2881 agacaaaaca aaatgacctc ttttatttaa gaaaggaggt cattttttgt atcataaatt
     2941 gtattaaaat caatgtatca ctttttaatc aattttaaga ccataaattg cgtactaaaa
     3001 gaaattctgc aattcaaaat aaagcatttt aagccatttt caaatttaag tgcacccatc
     3061 tacccgtcaa atcactttat tttccactcc agtagtttcc taattcaact gtgtgcaaaa
     3121 cgatttcttc taaacaaatc aagacaaact tataaacctt catcccagca ccataattat
     3181 ttaaatcata ggcatttttc gaaattagta tatgtactca agtttcactg cagattattt
     3241 atatttttta gagaaataaa tttttcctat tgacaaacac aaagaaagcg gatacaatac
     3301 aattatgtca catgacgtta tatgacgtta tgtaatatct ttacatttaa tcagttactt
     3361 ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga tggatgtagc
     3421 aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa tttatttggt
     3481 ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta aagccgaagc
     3541 atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa ccccaaagca
     3601 attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag cagaaacggt
     3661 aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga ctcataataa
     3721 aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg gagatgatgc
     3781 aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt tggtcaatgt
     3841 taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta tcgataccgt
     3901 aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa
     3961 agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg cttatggttt
     4021 ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc attctggaga
     4081 attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc tcttgatgag
     4141 cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata aatatgcaga
     4201 aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag atgaagtttc
     4261 tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt cagctttagc
     4321 agatgcacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg agtactagga
     4381 ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg
     4441 gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa aaactgggac
     4501 atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat gttttatcag
     4561 caataaaaaa aataggactg gacaacagtc actctcgctt tgaaaaaggc gagaatggct
     4621 gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat tcgggaggtg
     4681 ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta aaccagtttg
     4741 aattgattca tatgggatta tatagccatg tcaatcatct attagaagag ttacaaaatg
     4801 tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag gttcagcggg
     4861 caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat gaagaagtaa
     4921 agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca acccgaggag
     4981 aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc gtgctagaag
     5041 aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta ctttattttc
     5101 tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct gggaagtctt
     5161 gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt tttaaattcc
     5221 tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat
     5281 acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat
     5341 acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat agaaagttat
     5401 gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga agggtaccta
     5461 cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg taaaattgaa
     5521 tatgttatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac tagcattgtt
     5581 ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga tatcgctcaa
     5641 ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc cattatgtta
     5701 gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga gctgactgat
     5761 tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg ggaaactgca
     5821 cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc tgttgggacg
     5881 ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat tcatgtaggt
     5941 aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt tttgatttca
     6001 aggagtagat tttatttatg aagcaagtat tgcggtcaaa aatgattttt gatggagagc
     6061 agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag gtggaaaaca
     6121 actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat gatgatcaat
     6181 tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg gctatgaatg
     6241 ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt attaaagctg
     6301 taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat gctgccctgt
     6361 ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt ccaatcacag
     6421 cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag acttatggtt
     6481 ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa atgctagcaa
     6541 acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt tcaaaaggtg
     6601 ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat atacctcaga
     6661 ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag catgaacttc
     6721 tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga gtgaagatca
     6781 tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat cagtcaggag
     6841 tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag
     6901 tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa gctgcgtcaa
     6961 ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt agtgatttgg
     7021 aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat
     7081 atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca actgttataa
     7141 aaaat
//
LOCUS       DAZKAY010000116         6882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000116 DAZKAY010000000
VERSION     DAZKAY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.120"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8WP_002500"
     CDS             complement(<1..182)
                     /locus_tag="ACG8WP_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9028765.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8WP_002501"
     CDS             517..951
                     /locus_tag="ACG8WP_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9028766.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8WP_002502"
     CDS             974..1294
                     /locus_tag="ACG8WP_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028767.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8WP_002503"
     CDS             1435..1626
                     /locus_tag="ACG8WP_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028768.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8WP_002504"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8WP_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9028769.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8WP_002505"
     CDS             2112..2375
                     /locus_tag="ACG8WP_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028770.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8WP_002506"
     CDS             2399..2587
                     /locus_tag="ACG8WP_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028771.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8WP_002507"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8WP_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028772.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8WP_002508"
     CDS             3070..3441
                     /locus_tag="ACG8WP_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9028773.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8WP_002509"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8WP_002509"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8WP_002510"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8WP_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028774.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8WP_002511"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8WP_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9028775.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8WP_002512"
     CDS             5250..5447
                     /locus_tag="ACG8WP_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028776.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8WP_002513"
     CDS             5553..6035
                     /locus_tag="ACG8WP_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028777.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8WP_002514"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8WP_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9028778.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1058 c         1353 g         2140 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga at
//
LOCUS       DAZKAY010000117         6869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000117 DAZKAY010000000
VERSION     DAZKAY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.121"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8WP_002515"
     CDS             <1..227
                     /locus_tag="ACG8WP_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028779.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8WP_002516"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8WP_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8WP_002517"
     CDS             1635..1841
                     /locus_tag="ACG8WP_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028780.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8WP_002518"
     CDS             1862..2458
                     /locus_tag="ACG8WP_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFP9028781.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="ACG8WP_002519"
     CDS             3410..4009
                     /locus_tag="ACG8WP_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028782.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="ACG8WP_002520"
     CDS             4058..4774
                     /locus_tag="ACG8WP_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9028783.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="ACG8WP_002521"
     CDS             4788..5567
                     /locus_tag="ACG8WP_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9028784.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="ACG8WP_002522"
     CDS             5650..6006
                     /locus_tag="ACG8WP_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9028785.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="ACG8WP_002523"
     CDS             5996..6262
                     /locus_tag="ACG8WP_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028786.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="ACG8WP_002524"
     CDS             6421..6699
                     /locus_tag="ACG8WP_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028787.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="ACG8WP_002525"
     CDS             complement(6696..>6869)
                     /locus_tag="ACG8WP_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9028788.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DAZKAY010000118         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000118 DAZKAY010000000
VERSION     DAZKAY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.122"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8WP_002526"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8WP_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8WP_002527"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8WP_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9028789.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8WP_002528"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8WP_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8WP_002529"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8WP_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9028790.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8WP_002530"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8WP_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9028791.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8WP_002531"
     CDS             3582..4271
                     /locus_tag="ACG8WP_002531"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9028792.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8WP_002532"
     CDS             4285..5787
                     /locus_tag="ACG8WP_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9028793.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8WP_002533"
     CDS             5800..6276
                     /locus_tag="ACG8WP_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9028794.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8WP_002534"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8WP_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKAY010000119         6606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000119 DAZKAY010000000
VERSION     DAZKAY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.123"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..517
                     /locus_tag="ACG8WP_002535"
     CDS             80..517
                     /locus_tag="ACG8WP_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028795.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            612..1496
                     /locus_tag="ACG8WP_002536"
     CDS             612..1496
                     /locus_tag="ACG8WP_002536"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9028796.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1718..4291
                     /locus_tag="ACG8WP_002537"
     CDS             1718..4291
                     /locus_tag="ACG8WP_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9028797.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     K"
     gene            complement(4364..5911)
                     /locus_tag="ACG8WP_002538"
     CDS             complement(4364..5911)
                     /locus_tag="ACG8WP_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9028798.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(6013..6366)
                     /gene="tnpB"
                     /locus_tag="ACG8WP_002539"
     CDS             complement(6013..6366)
                     /gene="tnpB"
                     /locus_tag="ACG8WP_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9028799.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(6356..6550)
                     /locus_tag="ACG8WP_002540"
     CDS             complement(6356..6550)
                     /locus_tag="ACG8WP_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028800.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         2057 a         1207 c         1377 g         1965 t
ORIGIN      
        1 atccagccac cctagatatt tttctgtata atcttaaata aagaaaacga tattaaaaaa
       61 tgcacgtaga agtagataaa tggagtggtt ttgcaaattt cttttatttc tagaagttat
      121 tcacgtatac ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa tggatcattt
      181 gtatataaaa tatattcatc aattttccgt tttggtatta ggcacactaa ttatagcggg
      241 gattgttgca tttattattc aaggtcgtga catattaaca gtacatcaag agtttttcag
      301 ttatgcattt ggtattcctc tgatcgctat aaggtctgat aacaaaaagt ggaagaaacg
      361 ctattacatt ctcttgctat ttttgtgttt ttatctgcca cctaagattt ttactttatt
      421 aggtatacaa tgcctatcat tttcttttat ttcctatatc attgttttaa ttttcctcag
      481 tttcatagta tgggtatttt atcttgtcaa aaaataaatg aatgtttcat gaacagtact
      541 tcatcacagc ctaatttttt tctttatgct atcgttatat taaagaaatc aatcagaagg
      601 gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc cagttcaggc ggtgaacaag
      661 caaaggaatt tgagcaatta gcgatagcaa aattggaatc ggtgtttgat gaagtggtcg
      721 tcttgcatac aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg gctacggaag
      781 ggtatcatag tgtgtttgtc atgggcggag atggaacagt caacgaagga atcagtggaa
      841 tcgctgaaca agaacatcgg ccaaatttcg gatttttccc attgggtacg gtgaatgact
      901 tggctcgagc attagggata ccgctagaac ctgaggaagc gatcaatcat ttttcgattg
      961 aatcagtcaa gccattggat attggaaaaa taaatgacga ctattttatg aatgtcgtcg
     1021 caattggctc tatccctgaa gcaatcaatg atgtagatgc ggagaagaag acgaaatttg
     1081 gaaaactagc ttatttcatg tcaggaatca aacagttagc ttccacacaa tcctactcgt
     1141 ttcacgtaga agttgatgga aagaaggaag aaattgagag cagtatcttg ctgataggac
     1201 taactaactc agtaggcggt tttgaaacat tacttccaaa tgcaaaagtg gatgatggta
     1261 agctccattt ggtttattta aaagattctt ctttgatcga tacgcttaaa acattgccag
     1321 acctattaaa aggagtagat gaatctacga aaaatctagc ctatcttact tgtgagcaat
     1381 tagttgtgtc attagctgat gaggaagaat tgacgacgaa tgttgatgga gatgaagggg
     1441 ataaattacc tgtgacaatc aaagtgttac cctcacattt acaggtttat tgttaaaatg
     1501 gaggttgtga ccgaagagtt gtcaggagga catcggttgt tcggaataag ggactgtgac
     1561 aagacttttg tcacagcctt tttttgtaca aaaaagaaat tttcacctaa cagaaaaatt
     1621 tgccataaaa atacatattt taaatatgaa agtacaatta caaataaatt aaaacgttaa
     1681 aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatatg taaaaaagct
     1741 gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt ttgccgatgt
     1801 agagaaaaca agcgaatcaa cgcaatcaga gaatcaaagt tttgacaaga ctactttttc
     1861 agaagaatca attacttctg aaactacaga atcccaaagt gaggcgccta cttctaatac
     1921 aaggcaagaa gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag
     1981 cagtgaagag gtacaggtta gagttgtttc agcggatggg tacagcgaag ctgccacaac
     2041 agaagaactg acgcagcttc tcgcagacga atcagtagct aagattcgtt taactcagcc
     2101 tttgacttta gacagggaat tggagatcaa gcgagatatc gttatcgatt ttggcggact
     2161 tgctcataat ttcggcacac atcacatcta tatcaatgag acacctcagt cgatcgagtt
     2221 tcagaatttt aaaggaacgg cagctcatcc aggtgggttg ataccaaata tcgatggaaa
     2281 tgccattatc attgcttata tgtccgattt ttggggaaac agatatcatt ttacgggaga
     2341 aataaaattt accgggacac ttgacttata tgatggttct aaattaggcc tgatttacgc
     2401 ccctcgtgca actgtcacat tagatggtgt ttccggtgta ttggatgtcc aaccagtgaa
     2461 agaagggatg aatgccgctc cgggtaaagc ttatttttgt agaagctatc agctaaatgt
     2521 aatcaatggt agtcagcttt atggaccata tctaggaaaa ttctatggtt ttctagattc
     2581 aggtgatgag acgggcagtg gaaaaacagc accaggactc catatcttga gtggttcaaa
     2641 ggtctctctt gactatgatc gggcagatgg cgtcagcgat gaaggagagg ctgtcgatac
     2701 gctacccggg aatgtcacat ttaaagtcag tggctcagga agtgaatttt cggtaaatac
     2761 aaagatcaat attaccaacg acaacaatcg cggaattatc caaatgagag gctcaggatc
     2821 aagagtcgct gtgtcgaatg atgggaaaat cacaatcaat acagcgacta ctggcggatt
     2881 tcgcctgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa
     2941 aatggctgga gatggcgacc aacctggaaa caatgggatg cgttttgtag ggaaaggact
     3001 ttctttgatc gtagaaggag ccggatcagt catcgatatt gataagcaaa gtggtcgttc
     3061 tagcgcagtt cgttttgaga acggtacgca aaaactaaca gtaactaatg gaggctcatt
     3121 gaaggtaaga aatgcagggg atggcaaatc ttatgtgaac agactcaatc aaggaaacca
     3181 agcgatccaa ttttatgacg cttctggttt tctagaaagt ccagggtcag ctgattttac
     3241 ggttactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcta ccgtagatac
     3301 aactggagat attgatttga actttatggc tggcgaaaaa acttatttag tcatggtcgg
     3361 gaaaactggg gaagataaga ctggcatcat cagtggggat gtgttgaatg tagacttaga
     3421 atctcctacc tatttcgatt tccaaaacaa acggatcgga agcgagacta atggcatagg
     3481 tggctgggta tttcaaggaa atggcgattc ttctttgaag atcgctcatt cagaaattgc
     3541 tttatggcga aaggcaggag taacgttcga taactttgaa aaagatccgg attatttttc
     3601 tgatatgaca gacttatcga tcaatggaac cgacttaggt aattttgtat caagccctag
     3661 tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat
     3721 cagtgccaat aaccagcttc cgatgattga atccttaaga gtaccaacga atgcagataa
     3781 aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg
     3841 gacaggcgaa gtagaagtga ctttacgtat tacttatgca gatacgacga aatcacctgt
     3901 agaaattact gctctcacac aaggacaaac agatgaaaat gaagggacag gatataatcc
     3961 gtggggagaa ggcgaacaag gcggtttgtt tgaggttcaa gtacccaacg aagaaagact
     4021 aacaacgggg gatcaagtgc aaatagttgc tgctaaaaaa gtaagaggcg gccaagaagt
     4081 gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgagc ctgctaaatt
     4141 aagtaccaca ttgatcaatg cagcttctcg ctcaatcaca ggtacaggcg aagcaggagc
     4201 tttggtttca ttgaaaaaaa atcaagctgt tttgtccgaa acaactattg atgatacagg
     4261 taaatttgtt ctttctatcc cagaaaagta agcgccccat agaacagtac ctgtacgtca
     4321 aataacccga cctatagaat agaatcggga atgtttggcg tttttagtta cgagaaaaat
     4381 gttgttgtac atccttgttc caaggcaaca gttgatctaa agtttctgct gaaagtacgg
     4441 gaagatttgg tatcttatca aatagaaact ctagatactt tacaggattc aatccgttaa
     4501 gttctgctgt tttatataca ctcagaatga ttcctgaagt tcgtgctcct tcaagacttg
     4561 tggaatgcaa ccaatttttc cgtcctatca ccagctcttt gatatttcgc tccgcacgat
     4621 tatttgtgag ttctaaacga ccatcttcaa aaataggttc aaaaccactt cgtagtttac
     4681 acgcatattg aacggctgca tccaacttgg attttgcaac aggatctatc gttgccatcc
     4741 actcataaaa agcaataaat aatggacgca gttcttgttg ccgacagctt aatcgcttct
     4801 caggagaaaa gtctttccat ttcttttcta ggacaaacca tttatccaat tgtttgactg
     4861 cttgagctgc gggaatatcc gtgtttttct ttccttttgg aattgcttcg aaaaatttcc
     4921 gccgaacatg cgcgaaacaa tagacaagtc gtacactttc cacggcatta tacccgctat
     4981 agccgtcaca gtgtaaatac cctgtaaact ccttgagaaa agttttcgga acatctccag
     5041 cacgactttc gtcgtggtga taataaacga tgggatgctg actgtctttt cctgtacaaa
     5101 actgccagta ataggtatct gttttggcac tttcaatcac acgataactt gtttcatccc
     5161 cgtgtagaat ctcttcttga ttaagataga ctcttaatcg ttctcctaaa gggtctaacg
     5221 cgttttgcac tgctttaata tgccaattgg tgatattatc tctcgaaata tccaacccat
     5281 gtgtttgttt ccaatagttc tcttgccgat aggcaggaac tttctgacca aatttcatcc
     5341 ggatcgtttc cgccactaaa ctattggaac ctaagctatt agaaatcaaa ggtttaggaa
     5401 cgatcgcttt tttaaaagaa gaaagtcctt ctttttcgca gcttttacaa caataggtat
     5461 gttgataatg ggtgagacaa tccaacatag cctgatggaa acgaatttct tctcgtactt
     5521 ttgtccgtcc gatatctttc atctgtgaac cgcaatgttc acaaaattgt tcttccaaag
     5581 gaaggagatg ttctttctct gttataggta agtgtttgat tttgtctgct ttcgttccta
     5641 caactcgttg atgtcctttt actggaataa tggtggcagc tgcttctgga acacttacag
     5701 aaatttcttc atcactaaaa agattcaatt gcccattcgt ttcctcaaag agagtctctt
     5761 tagagcgtcc aaataatttg ttggttaaat aattgacctt ttcattcaat agttggattt
     5821 cagttgtaag attctgaatg gtttgttgtt gtgcttgatt ggttttatgg gcttcttgaa
     5881 gcaactgaag caacgtatct gtagtttcca aaatttttca ccaccttgaa tcgaactgat
     5941 tttgttacag aataccacaa gaaacgcaca agaaataggg gttcaacaca ttttttgtgt
     6001 taacccccca gcttaaaaaa ctcctttttt tcctggcaaa atagctcgag gttgatcgat
     6061 acttaatcct tctaacaacc atcgaacctg ttggttcgtt aaggttcgaa cttcgtcttt
     6121 cgtccgaggc cattttaact tgccgttttc tatccgctta taacagagaa gaaaaccatc
     6181 accgtcccaa tagagaagtt tgaaacggtc ttgacggttc ccgcaaaata aaaaaacggc
     6241 atcttcgtaa agctcgagtt gatactcttg ttgaataaga ctggcaagac catcaattcc
     6301 ttttcgcaaa tctgtttttc cgcacacaat ataaatatgt ttggtttgaa gtaaatcata
     6361 aagcattgga caacagttct ttcaaaatag cttgaagaat ataattgtta ataccgttat
     6421 agatcaaaac ttcagaacca ttctctcttt tgatgcgcat cgctaacttg gcagccgcgg
     6481 gtttaccttt ttctggtgca ctcttttcgt ttcctaattg gacaggaaca atctcattta
     6541 atttatacat agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt
     6601 gttagt
//
LOCUS       DAZKAY010000120         6347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000120 DAZKAY010000000
VERSION     DAZKAY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.124"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8WP_002541"
     CDS             290..1135
                     /locus_tag="ACG8WP_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9028801.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG8WP_002542"
     CDS             1416..2636
                     /locus_tag="ACG8WP_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9028802.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG8WP_002543"
     CDS             2633..5353
                     /locus_tag="ACG8WP_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028803.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG8WP_002544"
     CDS             5337..6281
                     /locus_tag="ACG8WP_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9028804.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAZKAY010000121         6306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000121 DAZKAY010000000
VERSION     DAZKAY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.125"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..1216)
                     /locus_tag="ACG8WP_002545"
     CDS             complement(86..1216)
                     /locus_tag="ACG8WP_002545"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9028805.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1276..1503)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1865..3067)
                     /locus_tag="ACG8WP_002546"
     CDS             complement(1865..3067)
                     /locus_tag="ACG8WP_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028806.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3127..3330)
                     /locus_tag="ACG8WP_002547"
     CDS             complement(3127..3330)
                     /locus_tag="ACG8WP_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9028807.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3472..4536)
                     /locus_tag="ACG8WP_002548"
     CDS             complement(3472..4536)
                     /locus_tag="ACG8WP_002548"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9028808.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4597..4704)
                     /locus_tag="ACG8WP_002549"
     CDS             complement(4597..4704)
                     /locus_tag="ACG8WP_002549"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028809.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4867..5679
                     /locus_tag="ACG8WP_002550"
     CDS             4867..5679
                     /locus_tag="ACG8WP_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028810.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6053..6244)
                     /locus_tag="ACG8WP_002551"
     CDS             complement(6053..6244)
                     /locus_tag="ACG8WP_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028811.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1868 a         1299 c          996 g         2143 t
ORIGIN      
        1 aaaaggtaaa taaaaaagag tggatttctc cactccatga aaagccgact ttttgggacg
       61 cttttttttg ccaagcctca catttttata gccaaacttc ttttgcgact tgctgcaata
      121 attggatttt cttccattgt tcttcttctg ttaaatggtt tccttcttct gttgaagcaa
      181 acccacattg aggactgata cttagatttt ccaatggtac aaattgttgt gcttctttta
      241 ttcgttcttt tattttctcc gtatcttcca aatctggaaa cttagaagta actaatccta
      301 aaacaacttt actttctttg ttactccaat gtcttaaagg agtaaaatct cctgctcggt
      361 cagaatcgta ttccaaaaag aaaccatcat aatttgttga aaacaactct tctgcaatgg
      421 gttcatatcc accggaaaac agccaagaag aagcgtgatt cccgcggcag atatgtgttg
      481 tgatcgtcaa atcttctggt ttgtcttcca gtgcgttatt gataatctct gtacaaatat
      541 gggccatttc tttcggatca attccttttt gaagaagtgc ttctctttta gtttgatcag
      601 ctagaaacgc ccaatttgta tcatcgaatt gcaggtaacg gcaacctttt tcataaaaag
      661 catgaatcgc tgctttgtaa gtagaggaca agtcctcata aaaatcagat agatcaggat
      721 aaatctcctt gatttttgaa gaaccatcat tagataggat ctcttgtcgt aaaatcatcg
      781 tgggactagg gatcgttgct tttgcaacaa aagtatcatc tgtttccact acttctttca
      841 gaaaagcaaa atcttttaaa aatgggtgat ttggattgaa agataattta tctactaccc
      901 gaatattata agctggtgct gcttttccgt ggaatgtttg attatatcca gtttctggaa
      961 catagccttc gataccattt agttcttcta aaaaatcaag atgccaaaat ccacgccgaa
     1021 attcaccatc tgtaattcct ttgatgccga ctgctttttg gtcttccact aattttttta
     1081 tttcctggtc ttcgattgca cgaagttctt cttttgaaag taagccttct tgaacttttt
     1141 ttcttgcttc tttgatcgat tgcgggcgta agaaacttcc gacatgatct actgtaaatg
     1201 gtctattttt gctcatttca ctcactcttt tctttgtttt ttgtaaaaaa aaatcctttt
     1261 actagcaaat actagtaaaa ggacgattat actcgtggta ccaccttttt tcagaaaaaa
     1321 cattctttct ctcagcctga caataaaatc agtggtcttg gtaacgaaag actgttcgtt
     1381 tttgcctacc tttcgacaaa aaatcctcta aggccatttt cgacagtcaa cttctagctt
     1441 tttacaccaa ccaaagcctc tcttgaacca ttttctgtct actttccttc tcatcggata
     1501 ctattagaat aaaattagaa tagcatgttt gaaagaaaag tcaataggga ataagaacaa
     1561 acaaaaacta atttgttatt cttagtaatt tgtttgtgga atacaataaa gcttttctaa
     1621 tatgataatt gttcagtcgt cctttcatct tagatcactt ccttaaaatg atcatacccg
     1681 tttcaaccac ataaaaaagg acttaatttt gaaaaatttc ccatcaaaac taagtccttt
     1741 ttattatagt tatgcaaatt tgcttatttt ctcgctattt ccgatacatc ttgaacagga
     1801 gcaattcgac tgaaaacact ggtgcaaaag catttatttg tcttatggta gaaccgtgga
     1861 aaactcagac tggtaataaa ttgtcatttt ttgaaagaat agcgccctct tgaacattat
     1921 caatcgcact taccacttgt tgatctgact ttgcatataa gtgaccgtaa gttcctaaag
     1981 tgatttgaat attcgcatgg cccaatcgtt cttgaataga tagcggttga atattctgct
     2041 caattagaaa tgcaacatga gaatgtcgta gcgcgtgaag cttaatcggt ttgacacccg
     2101 cttttttagc agcttctttt aaccagttcg caaaagaata aggtggcgta aatgttccgt
     2161 ccaactgagc aacatattca aaatttccaa tacgtttttg caagtcatac cactgtctga
     2221 gttttaagga aagcttcttc cccaacccaa tcgttcgata agcacattta gttttgggag
     2281 tcgaaaagta ccaatcatct tttgtattat aaatcaatgt cttctcaatc gttatcgtgt
     2341 ttttctcaaa atctacattc gaccaagaca aagcttcaag ttctccaatc cgaatgccag
     2401 tataaaagag catttcatac acaatcttcc gatggtgggt ttgaatctta tcatccttca
     2461 attcattcat cactttttga aactcactgt gttcccaaaa ctcaactttc ttattctcag
     2521 aaggaagcga agaaacatct tcaacgggat ttttagcaat caccccctct ttaacagccc
     2581 gatccaatac aatttttagg tgccctcgaa tccgattgat ataagatttt gataacgttt
     2641 cgccattaac ttttttccgt gaattacgtg actctgtctg gcttagttca aataaccaat
     2701 cctcaatatc gtgacttgta atatctcgaa cacgaaaatg atagaaaaac tgcatacgct
     2761 tttcgaaaat gaattgcgca ttctcatatg tttgactttt gacctgtctc tcgtaccatt
     2821 ttacaaacct atccttgtaa aactcttcga aagtcatttg attggtagaa cacagttgat
     2881 taggaattgt cttgttaaaa tcatgtgttt ccatgtactt gtcataagca attttggcat
     2941 ctttttgtga agcataacct cgtttagtct tctgaacctt tcttccattt tcatctatac
     3001 ctagatacgc tcggaagaac catttttttg tctttttatc ttggtaaata tttttaattt
     3061 ttggcatcgt tccattcctc cctgtacttg agaaaagcta ctttaatagt accgcgcttg
     3121 tactgtttat tccgcaactt gaatgtgaaa aagatgttca attactcggg atggcaccac
     3181 accaacttgg cgattattat aaaaatcaat accttcaatc cttgcaagat atgccttgct
     3241 ctctttaatc attcgtgcag cttgactagg tttaaagccc atctctatca aaattgcacg
     3301 atttaccatg aaatcacatt ttgtttccat tctctcacct cctgattctt tactcctttt
     3361 aaaataatcc tctttataat ctattgtttc tcattcgatg gttgtgttat actcgtcttg
     3421 tcgaggcgga gtacgttgca caacttgttg tgtaagcagt actttgcttt tttagtagct
     3481 tgttttctca tttgatatac tttgtaattt tcgttcataa tactcaacct cctcctgata
     3541 atactccggg tctgccttca tattttggag aagtttgatc tgtcttggat tcatttctcc
     3601 ttgttcaata atcaaaggca acaaggaaga atgaaacgtt tgatcgacct gtgcaataaa
     3661 aactaatgat ggtgaaacac catgaattaa ccaactcata gttttctcaa aactttgagg
     3721 aattgtatct aaagaaatgg tgattgcgcc ataattgcta ataaaggctg cccatttggc
     3781 atcaactttt gcacctagat catttggatc acggtcataa aaacaaattg ctccattaag
     3841 taactcaaag aacagtttgg ttaaatcatg ggtttttgaa ataatctttg ccaagctttg
     3901 cgctttctct tttcgatacc gtagttcaaa gcgattcttt acttttgcct cttctaaagg
     3961 aattcctcgt cgcttccgtt gttcatggtc cttctgataa aagcaaaaac gacactgact
     4021 cttctttgag cctaaataca aggtctcacc atttgattca ttgtttatga tatccgtacc
     4081 gccatgagat tcactcactt gaaaagtggt ccacacatgg cctagtatta ccttatgctt
     4141 aagttctgga atgctgagac tccccacaaa atcatctaaa gtaaaatcaa tccgtgtaaa
     4201 gttcccctga taatctaata ccttacgaaa aaactgttgc caatttgttt ttcgtgtttt
     4261 tagccatctt gctaagtgtt tacagccttg acctgaaaac tccatcacag ttcctttaac
     4321 cgcatcgtca atggaatacc gaatcgtgat tacttgattc cacacgtatt ggccaatata
     4381 gccctttggc gcataatcca gttcctcaaa acgttcaatg ggcatttcaa taatctcttc
     4441 aattagcttt aaatgttgat tcgagaaaaa gtgaatggtt agataatcaa tcaaaacacg
     4501 gttaactgga acgacctctt cttgcaaact atccaacaca agattcaatt tttcttcact
     4561 taattgtctc gttccttttt ctactttcga taataactat ctcgaaatcc ccactttttc
     4621 tgctaaatat cgttgtgtcc accccctttc ttctcgagaa actcgaatgg cttcaccgag
     4681 atcatgttga ttaacttggt tcatcgacaa acctccttct gctatttact tgtcaaagaa
     4741 cctattgaac ttattttaag tacattttta ttgtacgcct gtatttattt taagtcaata
     4801 tgttttgact ttttttaagt acacacgtat actaaattta agaacttatc ctgaggagga
     4861 gttttcatgg agatagacaa gcaagcagtc ggcaatcgaa ttcggcagat tcgccaagaa
     4921 ttaaagttga gtatggagaa atttggcaaa ctaattggtg atttaccgcg aagtacagtc
     4981 aataactggg aacgtgggat caaccttcca aaaaccgaaa cccttcacca aatcgcagag
     5041 ataggtcatg tcaccaatga atatcttttg tatggtgatc aagagaatca atacatttta
     5101 gaaatgctcc aaaaaaaggc aggtaagctt gatcctaaga ttgaaggtct catagtagac
     5161 gaaatcaaac acgctgacct tgttagtgaa aaagaaatgg atcgaatgat tgaatttttt
     5221 attacaaatc tcattccacc aactgaacaa gaccagttta ctttccaatg tatcgatgaa
     5281 aaaaagcagc tttatcttgg ttcaactaat tttggtaagg aagcccagct ctatttgcaa
     5341 catgataatc aaaatcatat tctgcacatg atgcccttta ccttctccaa ctttagtgtg
     5401 gatcgcatac tcgtctattt agcaaatcaa aaatcctatt cctattttgg gaaacattta
     5461 cccaaggaac taggtgagaa agccatcctt ctatactcaa ttaatcaatt gaccagcgat
     5521 gtgcgaattg ccccgttggt ctattcagaa gaaaccgctt cttatcagta cacagaggac
     5581 aatcaatatc tactagaaca gcaatatctt tatctaccat tcgtgatgga agtcgagaaa
     5641 gaacgcctgt taaatgcagc gtatccatca tcaaaatgag gtgacaaaat tgataagcac
     5701 gtgacaaaga aaatactgct aggacactcc ccaatgggta gttgtcacag tttcctgttc
     5761 tttgttagcc ccctgttaga accgggggct ttattcctgc agtccggcta cgccttcctg
     5821 caatagacaa aactccagat cgggtaattc gcttgtggcg aaacccgacc tggagtttgt
     5881 ctctgtattt tgaaccctaa aaattaatca aacagactga tattctcata atcagcgcga
     5941 aataagccat taagtacagt tttcctacct atttttgata acccgctggt tagttaatat
     6001 tccttttccg agagagataa atgattggtt aaatctatat acatctcaac ctttaaaaat
     6061 gttttcgaaa tacttcatca gtacatgtca tacaaattac cagtgcattc tcaaattcta
     6121 gttctttaat tgatttcagt tgaggagcag ttacttttgg cggaaatttc catttttgtc
     6181 caaacctctt cccccgatga aatttctttg ttgcaattat tgcaatgaaa aatttttgtt
     6241 tcaaaaatat acataattac ttcctccaat aaaaactatc tacctctcaa attatttttc
     6301 tctaat
//
LOCUS       DAZKAY010000122         6264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000122 DAZKAY010000000
VERSION     DAZKAY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.126"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..228)
                     /gene="rpmB"
                     /locus_tag="ACG8WP_002552"
     CDS             complement(40..228)
                     /gene="rpmB"
                     /locus_tag="ACG8WP_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9028812.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            498..860
                     /locus_tag="ACG8WP_002553"
     CDS             498..860
                     /locus_tag="ACG8WP_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9028813.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            912..2594
                     /locus_tag="ACG8WP_002554"
     CDS             912..2594
                     /locus_tag="ACG8WP_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9028814.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2711..4747
                     /gene="recG"
                     /locus_tag="ACG8WP_002555"
     CDS             2711..4747
                     /gene="recG"
                     /locus_tag="ACG8WP_002555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9028815.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4796..5797
                     /gene="plsX"
                     /locus_tag="ACG8WP_002556"
     CDS             4796..5797
                     /gene="plsX"
                     /locus_tag="ACG8WP_002556"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9028816.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5919..6161
                     /gene="acpP"
                     /locus_tag="ACG8WP_002557"
     CDS             5919..6161
                     /gene="acpP"
                     /locus_tag="ACG8WP_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9028817.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2078 a         1092 c         1429 g         1665 t
ORIGIN      
        1 aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc
       61 tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg
      121 aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt
      181 gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc
      241 ctttgccttg atttggagca atagaataat tcagctcata ccagactaac atatcatata
      301 gaatggaaat ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt
      361 aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc
      421 ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc
      481 gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta
      541 ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag
      601 gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt
      661 actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga
      721 ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat
      781 acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg
      841 ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga
      901 ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg
      961 caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg
     1021 tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag
     1081 tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc
     1141 tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta
     1201 agcaaattcc agatgttgtc tcattaaatg cttccgatct tgctgcagcc ttcactcatg
     1261 gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag
     1321 ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag
     1381 taatgacagc tgttgtaaaa ggggcaaagc gtgctttagc taaaactcca gacttgcttc
     1441 cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg
     1501 agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc
     1561 cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg
     1621 cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac
     1681 ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag
     1741 attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc
     1801 caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca
     1861 acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga
     1921 aaaaagaaac accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc
     1981 aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtgggcaa acaatgaatc
     2041 caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct
     2101 tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc
     2161 cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca
     2221 atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg
     2281 tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag
     2341 aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag
     2401 caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct
     2461 tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa
     2521 aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat
     2581 tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag
     2641 gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aagggaggga
     2701 ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa
     2761 aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta
     2821 tcctttccgc tacgaagata ttcaggaaag aaatctaaac gagatccaag atcaagaaaa
     2881 ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa
     2941 aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa
     3001 tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg
     3061 ggatgcaaag cgtaaagcat tgaacgggat gaagatttta ggttctcaaa caatggatga
     3121 cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat
     3181 caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt
     3241 ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga
     3301 ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca
     3361 aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct
     3421 ttatgatgtc caaaaattga aagaatttac tcaaaagcta ccttttgaac tgacaaatgc
     3481 acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg
     3541 acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc
     3601 taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca
     3661 acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgcttac
     3721 ggggtcaacg aaagcaaaag aacgtcgtga gctccttgaa aaactagagc aaggggaaat
     3781 taacatcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg
     3841 tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga
     3901 aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc
     3961 tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat
     4021 cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa
     4081 aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga
     4141 agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc
     4201 aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat
     4261 ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg
     4321 ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc
     4381 acagctgcat caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt
     4441 agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac
     4501 caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg
     4561 cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt
     4621 tttggaggta gccagagaag aggctgcagc cctttggcag ataaaggatt ggcagatgct
     4681 tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt
     4741 cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa
     4801 aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc
     4861 tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat
     4921 caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa
     4981 tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc
     5041 tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc
     5101 gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt
     5161 gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg
     5221 cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta
     5281 tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga
     5341 agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag
     5401 cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt
     5461 ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa
     5521 tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc
     5581 tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg
     5641 cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc
     5701 cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg
     5761 acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcaatcc
     5821 gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta
     5881 ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta
     5941 acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg
     6001 aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag
     6061 agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag
     6121 tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga
     6181 aggataaaaa gctgcataca gctttttatc cttctttttt taatcttttt gacacgtaaa
     6241 agtcattttt ctgataattc tggc
//
LOCUS       DAZKAY010000123         6230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000123 DAZKAY010000000
VERSION     DAZKAY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.127"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG8WP_002558"
     CDS             133..633
                     /locus_tag="ACG8WP_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9028818.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG8WP_002559"
     CDS             730..2577
                     /locus_tag="ACG8WP_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9028819.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG8WP_002560"
     CDS             2580..3476
                     /locus_tag="ACG8WP_002560"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028820.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG8WP_002561"
     CDS             3526..3915
                     /locus_tag="ACG8WP_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9028821.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG8WP_002562"
     CDS             3902..>6230
                     /locus_tag="ACG8WP_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028822.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZKAY010000124         6065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000124 DAZKAY010000000
VERSION     DAZKAY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.128"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8WP_002563"
     CDS             832..1566
                     /locus_tag="ACG8WP_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028823.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8WP_002564"
     CDS             1582..2751
                     /locus_tag="ACG8WP_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9028824.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8WP_002565"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8WP_002565"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9028825.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8WP_002566"
     CDS             3841..4767
                     /locus_tag="ACG8WP_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9028826.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8WP_002567"
     CDS             4786..6036
                     /locus_tag="ACG8WP_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9028827.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DAZKAY010000125         6020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000125 DAZKAY010000000
VERSION     DAZKAY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.129"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..362)
                     /locus_tag="ACG8WP_002568"
     CDS             complement(60..362)
                     /locus_tag="ACG8WP_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9028828.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8WP_002569"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8WP_002569"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="ACG8WP_002570"
     CDS             complement(1119..1892)
                     /locus_tag="ACG8WP_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9028829.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="ACG8WP_002571"
     CDS             2036..2386
                     /locus_tag="ACG8WP_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9028830.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="ACG8WP_002572"
     CDS             2367..3083
                     /locus_tag="ACG8WP_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9028831.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="ACG8WP_002573"
     CDS             3083..4531
                     /locus_tag="ACG8WP_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9028832.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="ACG8WP_002574"
     CDS             complement(4602..5111)
                     /locus_tag="ACG8WP_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028833.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5101..5825)
                     /locus_tag="ACG8WP_002575"
                     /pseudo
     CDS             complement(5101..5825)
                     /locus_tag="ACG8WP_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DAZKAY010000126         5999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000126 DAZKAY010000000
VERSION     DAZKAY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.130"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..477)
                     /gene="yajC"
                     /locus_tag="ACG8WP_002576"
     CDS             complement(97..477)
                     /gene="yajC"
                     /locus_tag="ACG8WP_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9028834.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(574..1719)
                     /gene="tgt"
                     /locus_tag="ACG8WP_002577"
     CDS             complement(574..1719)
                     /gene="tgt"
                     /locus_tag="ACG8WP_002577"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9028835.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1937..2635
                     /locus_tag="ACG8WP_002578"
     CDS             1937..2635
                     /locus_tag="ACG8WP_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9028836.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2803..5442)
                     /locus_tag="ACG8WP_002579"
     CDS             complement(2803..5442)
                     /locus_tag="ACG8WP_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9028837.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1685 a         1318 c         1103 g         1893 t
ORIGIN      
        1 gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct
       61 ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt
      121 ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt
      181 tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat
      241 tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc
      301 attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg
      361 tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat
      421 aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt
      481 tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac
      541 tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt
      601 gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc
      661 ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg
      721 aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt
      781 acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt
      841 tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg
      901 caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc
      961 accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa
     1021 tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg
     1081 gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc
     1141 ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc
     1201 tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg
     1261 tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc
     1321 tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc
     1381 ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa
     1441 aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc
     1501 accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt
     1561 caactcttct ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg
     1621 tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc
     1681 tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat
     1741 aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc
     1801 ggcttccttc tactttatct aatattcttt tttttatcct ttttttatga ataacaaaaa
     1861 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
     1921 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
     1981 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
     2041 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
     2101 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
     2161 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
     2221 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
     2281 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
     2341 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
     2401 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
     2461 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
     2521 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
     2581 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
     2641 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
     2701 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
     2761 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
     2821 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
     2881 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
     2941 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
     3001 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
     3061 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
     3121 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
     3181 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
     3241 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
     3301 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
     3361 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
     3421 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
     3481 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
     3541 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
     3601 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
     3661 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
     3721 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
     3781 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
     3841 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
     3901 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
     3961 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
     4021 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
     4081 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
     4141 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
     4201 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
     4261 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
     4321 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
     4381 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
     4441 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
     4501 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
     4561 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
     4621 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
     4681 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
     4741 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
     4801 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
     4861 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
     4921 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
     4981 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
     5041 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
     5101 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
     5161 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
     5221 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
     5281 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
     5341 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
     5401 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
     5461 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
     5521 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
     5581 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
     5641 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
     5701 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
     5761 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
     5821 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
     5881 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
     5941 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggcttttt
//
LOCUS       DAZKAY010000127         5966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000127 DAZKAY010000000
VERSION     DAZKAY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.131"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..911)
                     /locus_tag="ACG8WP_002580"
     CDS             complement(<1..911)
                     /locus_tag="ACG8WP_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9028838.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKT"
     gene            complement(913..2352)
                     /locus_tag="ACG8WP_002581"
     CDS             complement(913..2352)
                     /locus_tag="ACG8WP_002581"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9028839.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2537..4483
                     /locus_tag="ACG8WP_002582"
     CDS             2537..4483
                     /locus_tag="ACG8WP_002582"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9028840.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4747..5619
                     /gene="scrK"
                     /locus_tag="ACG8WP_002583"
     CDS             4747..5619
                     /gene="scrK"
                     /locus_tag="ACG8WP_002583"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9028841.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1892 a         1021 c         1071 g         1982 t
ORIGIN      
        1 gtcttttcgc ctttaatttt tttcagaaga atatcaacaa taagacttgc aatttcatca
       61 attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga ggtaccatcg
      121 tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc aattttcata
      181 gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg atttataata
      241 gttgacttta tttccatttc acgacggata gtagacagat tattaggaat tttgaaaatt
      301 tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg agaatccgtg
      361 ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa agtcttagct
      421 gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg agctaaattt
      481 ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc aagattatgg
      541 cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta ttctctctct
      601 ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt aaataactct
      661 atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg aaaaataaga
      721 ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg tttataacca
      781 agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata tcccttatta
      841 ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac atcagttaat
      901 tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc ctgatttgat
      961 ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt tttctccttt
     1021 attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg tttctttagt
     1081 atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc ctgctttgct
     1141 gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc catttccttt
     1201 actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta gttcaagctc
     1261 ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac gtaatgatgt
     1321 aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt cttttacaag
     1381 actcattgct ccctgataat catacttatc agttggataa tcgatatcag gtagtccaat
     1441 ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg catacgcttc
     1501 aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt tttctatgtc
     1561 aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta gctcagattt
     1621 atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata ctagatttgg
     1681 acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa cttcctccca
     1741 tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt tttctaaatt
     1801 ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg gatcacgaaa
     1861 atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg taatgttgta
     1921 gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt catttcgaac
     1981 gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac cggaataagc
     2041 gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat gaaaatgaac
     2101 taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc caaatggcca
     2161 attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag gatcgtttaa
     2221 caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt ttacattatc
     2281 cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc gtatttctcg
     2341 aggtaagttc atattttact ccttcaattt atctttttac aactagtatg gtaaacgttt
     2401 tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa cgtttgacat
     2461 atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac gtaaaaataa
     2521 aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac aagcggtcgg
     2581 aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg tactaaaaga
     2641 tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaaa gcacatttaa
     2701 aattgatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg tttatgacga
     2761 acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac aaattgctag
     2821 taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag atatttttgt
     2881 tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa actttttaac
     2941 agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga ttgaaggtat
     3001 ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa ttttagtagg
     3061 tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa ttggtatgat
     3121 catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg gttatcatgt
     3181 ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt ggatattatc
     3241 tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta cattcacgcc
     3301 attattatca gtaatgatta ctggattttt gactttcaca gttattggcc ctgtaatgtt
     3361 attgttgtct aatggaatta cagacattat tgtttggcta tataacacta ctggatttat
     3421 tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt tacatcaatc
     3481 atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg gacatggaga
     3541 ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa catttgccat
     3601 ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg gtgtctcagc
     3661 acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc gctttccatt
     3721 cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac ttaaagtagt
     3781 tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg caacatctat
     3841 tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa ttacttactt
     3901 ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg aacaaacacc
     3961 agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg aatcaacaca
     4021 aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga ttagtctttc
     4081 ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg ccattaaacc
     4141 aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt ttgaaactgg
     4201 acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata ttggtattga
     4261 tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc aaaaagttag
     4321 aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag gtcttgatga
     4381 tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata tagtagctga
     4441 aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt tccctgagta
     4501 aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt tgagaataac
     4561 attttattta atcttttcat atatcaattg tatgaaaaat tattagaatt ttaaaaaatg
     4621 ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catgttacta aataaaaaaa
     4681 taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa
     4741 atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc
     4801 gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca
     4861 atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt
     4921 ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg
     4981 accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg
     5041 atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc
     5101 agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt
     5161 gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc
     5221 gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta
     5281 gaagggatgg ccgctggacc cgccatcgaa aaccgcttag ggaaaaaagg acaagaggtt
     5341 gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat
     5401 acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc
     5461 catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca
     5521 cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt
     5581 tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca
     5641 tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg
     5701 cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg
     5761 aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacaaa
     5821 aaaaaggggg tgcggatatt tttttggacc tttttaacaa ataggttctg ttgcaaagtt
     5881 ttaaatctac tatcagataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     5941 agtcatttta aaggaaagca atttca
//
LOCUS       DAZKAY010000128         5812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000128 DAZKAY010000000
VERSION     DAZKAY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.132"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG8WP_002584"
     CDS             complement(<1..497)
                     /locus_tag="ACG8WP_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP9028842.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            567..1973
                     /locus_tag="ACG8WP_002585"
     CDS             567..1973
                     /locus_tag="ACG8WP_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028843.1"
                     /translation="MDFFIYTKYFILSSFYYGEKFFKKIDAAFLFSCLYTFATYRTVD
                     IYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSAL
                     IACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFK
                     TDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVS
                     TWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLII
                     GATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKE
                     RPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVV
                     NDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL
                     KDSRYVTKKEINKKNENK"
     gene            1960..3594
                     /locus_tag="ACG8WP_002586"
     CDS             1960..3594
                     /locus_tag="ACG8WP_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP9028844.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3769..4602
                     /gene="msrA"
                     /locus_tag="ACG8WP_002587"
     CDS             3769..4602
                     /gene="msrA"
                     /locus_tag="ACG8WP_002587"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9028845.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4682..5671
                     /locus_tag="ACG8WP_002588"
     CDS             4682..5671
                     /locus_tag="ACG8WP_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9028846.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1995 a          905 c         1057 g         1855 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctagttt ttattatgga gaaaagtttt ttaaaaagat agatgctgct tttttattta
      661 gttgtttata tacttttgca acttatcgaa cagttgatat ctattatcga tccgcaattg
      721 cagaagcaat cgctcttacg attatcccag ctgttttata ttatgcttat ggattgatat
      781 tcaatagaga aaaaaaagtc atcgctttat ctgtcagtat aagtctactt gtgtatactc
      841 atgtcttaac tacactattg tgtagtgcat tgattgcttg tatgattata gttcgtttgt
      901 ttttaagcaa gcaaataaaa aacgatataa aatttgtggt cgtgaattta gtaaaggctg
      961 tttttttgac ttgtgcatta actagctatt tttggtatcc tatgttgcag cagatgaatt
     1021 atcaagaaat acacagtccc ttcaaaacag atttgcagct agaagcactt tctatttcag
     1081 atagtatcat tcaagcgtta aataacgata ttacaacata cactatgggg ttattaggat
     1141 tggttgcgtt gatttttcct ttattcatac tgaaagaaat ggagaaaaaa gaaaaaatca
     1201 tctatgttgg cgtagtttct acatgggtat gcgtaactaa tttatttcca tggtttttgt
     1261 ttcaaaaaac accgataagc ttgctccagt ttccttggcg tatcttaggt atccaaatct
     1321 ttttttcttc aatgatcata acgcttgttt ttatgaaaaa gactatgaat aaaaaatatt
     1381 catggttaat cattggcgct actgtggtgt ttctactcgt cacgtctata gcagcaaaag
     1441 aaaattacgc aaaagttatc caatcaaaac acggtcatat tgttatcaat gaagaaacac
     1501 tcccacgtta tacaacatca gatatgggag gattatacga ttacgcccca acagaagcga
     1561 taaaagaaag gcctcactta gaaaaacatg aagtaaaagt aggagaatcg tgggaaaaag
     1621 ggaagtataa agctgcagat aataacatta cttacacagt ggcaagcaac aaaaagcaaa
     1681 aagtgacagt accagtatat gcttatttag gtacacaggt aaaagtaaac ggtaaagtcg
     1741 taaatgactc ttataaaaaa catggcttag tgaatgtgga tgtaaatgct ggagaaaata
     1801 agatagaaat tacaacaaaa tatactccaa ctgctctcat ggctttatgg atcagtattc
     1861 tgacttattg tttagtggtg tattcaagtt ttaaacgatt tgcacctaat aagttgaaag
     1921 attctcgata tgtgactaaa aaggagataa acaagaagaa tgaaaataaa taaaaaagta
     1981 gtaatattga gtggattaat tatagttagt agtatatatt ctttcatctt tttcaaaaat
     2041 acacagagta tttataccac tggagatctt tcttttcact tatctcgaat caaggggtta
     2101 tcgactattt ttaccagtcc gattaattat gagacattca attatactgg atatggtgta
     2161 aattattttt atccctttct gacttttttt ccagctgtaa tgttatattg gatgacaaaa
     2221 aatttgattg taagctatat tatttacgtt tggttattga acttgtgtac aacactcgta
     2281 gcttatcatt atggcgagcg gtttttaaaa caaaaaaaag cggcattttt atttagttgt
     2341 ctctatattt tctctgctta tcggacagta gacatctact atcgttccgc aattgccgaa
     2401 gctatcgcaa taacacttgt tatccctgta ttattttatg cttatcaaat catctctgga
     2461 aaagaagaaa agtatccgtc tgtaaagcta gcattgagta tgagtttgct agtgtacagc
     2521 catgtactat caacattaat gagtacagca ttaattatca tttttatttt tattcgtttg
     2581 gtttctaaag gtttcaagaa tgcagatttt attgctatat ttaaaaaatt atttagtgcg
     2641 gcaggaatga cattggttct gacgagtgct ttttggtatc cgatgtttga acaaatgctc
     2701 taccaaaaaa tcaacaaacc tagcgtgaca aatttatatg ctcatgcatc taatgtgttc
     2761 gatagtctga ctgaagctat gaacaacgac ttaactactt actcaatggg actagttggt
     2821 ctgttatctt tatgtattcc cctgatttta tttaaaaaat taacaaacat agaaaaaaag
     2881 atttattatg gtacgtgttt gacttggcta gctacaacta gtttggtgcc atggtatctt
     2941 ctgcaaaata cgccagccaa attgttacaa tttccttggc ggatcttaag cttgcaaata
     3001 atattctctt ctctgatatt aacgatgata tttttcaaaa atagaagata caataagaca
     3061 agagaactat tttatttatt tatgactgtt ttgttattaa tcactttaac tgtttctagt
     3121 aaaaaaaatt tcaatcaaaa aataatttct cagccgggac atattgttgt aaacaaagaa
     3181 acagtagagg cgtataccac taaaggaatg gattattttt tatatgatta ttctcctatc
     3241 gcttcattgg agaataagga gcatataata aaacacgaag caaagattgg cagtcaatgg
     3301 gtaaaccttc cttataaaac aactggtagt tctatcatat atagagtaac taatgagaag
     3361 agccaagtaa tcacacttcc tgtatatgct tatttaggaa cgaatgtaga aataaatcat
     3421 caacctgtaa aggagcaggt gaatgctaat ggattggtac aaataagcgg agtcaaagga
     3481 gttgctgata ttaaagtaag cacaagttat acagtaacga ctattatttc ttatcttgtt
     3541 acattaactg catatttatg tttatttttt aaaatagttt tccataaaag gtaaattaac
     3601 ttttcattta taaaataaat tgattaaaaa ggagaatctg tcgataagga ttctctcttt
     3661 tttatacgaa aaatctgtag ggggatgaag taatcgctta tttcctcact ttatgataaa
     3721 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
     3781 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
     3841 cgaaaagtat tgattcagac aaagaatgac ataagagaaa atatatccgt taaagaacag
     3901 ttatcaaaac tagaagcaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
     3961 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
     4021 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
     4081 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
     4141 tacacgggcg gacatgttga aaaaccaaat tatgatcaag taagtggtgg ttacacaggt
     4201 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
     4261 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
     4321 aagcaatatc gtccagtgat tttttatcaa ggcgaacgac aaaaagaatt agcggaacaa
     4381 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
     4441 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
     4501 aaacgttata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
     4561 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
     4621 tagtaaacta aaaatagaaa aaagaaagcg ttttaaaaag atttgatgag gaggagaaaa
     4681 attgtatatt gcagatagtg cacgttatga aaaaatggaa tatcgtaaat ccggtcattc
     4741 aggtttgaaa ttaccgattt tatctctcgg attatggcaa aattttggtg attatgatcc
     4801 tatccataat caaagagaga ttttaagagg cgcattcgac atgggaatta cccatttcga
     4861 ccttgcgaat aattatggag gccctgctgg agctgcagag aaaaactttg ggcgtatttt
     4921 tagagaagat tttcaagcct accgtgatga actgattatt tctagcaaag caggttatca
     4981 tatgtgggaa gggccatatg gggaatgggg ctctcgtaaa tccatcattt caagttgtga
     5041 ccaaagtctc caaagaatgg ggctagatta tgttgacatc ttctatcatc atcgcccaga
     5101 ccctgataca cctttggaag aaacagcaga agctttgatg caacttgtaa gacaaggaaa
     5161 agcattatat attggtatat ccaactataa tggagaagat acaaaaaaaa tgactgaaat
     5221 tttaaaaaga aaggaagcac cgttcattat tcatcagatg cgctacaata tgttttctcg
     5281 tgcactacta gaagacgatc tatctccagt attagaagaa gaagggctag gggcaatcac
     5341 gttcagtccg cttgctcaag ggttgctgac aaatcgttat ctacatggta ttcctgaaga
     5401 ttctagagca catcgtaagg aaataccatt cctttcagaa gaacaagttg gatcaactct
     5461 ggaaaaaatc aaagcactgc aaacaatcgc cgtatcacga ggacaatcat tagcgcaaat
     5521 ggcccttgca tggaatttgc gacaaaaatc tgttacaagt gtactggtcg gagcaagtcg
     5581 gttaagtcag ttacaagaaa gtgttcgaat gatggataat ttagatttct caccagaaga
     5641 agaattgaga atcgaccaaa ttctagaata gaaacgatgt aatcaaactt tttatccata
     5701 tattaaaaaa aacaaaagat gacatcaaga atcgaatgaa gatcgtctgt tattgaacat
     5761 catatttttt gtcgttcaaa ataaaaatga tcgaaggcta ggagtgaatt tt
//
LOCUS       DAZKAY010000129         5771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000129 DAZKAY010000000
VERSION     DAZKAY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.133"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..1167
                     /locus_tag="ACG8WP_002589"
     CDS             13..1167
                     /locus_tag="ACG8WP_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9028847.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1140..1583
                     /locus_tag="ACG8WP_002590"
     CDS             1140..1583
                     /locus_tag="ACG8WP_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028848.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2717..3685
                     /locus_tag="ACG8WP_002591"
     CDS             2717..3685
                     /locus_tag="ACG8WP_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9028849.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3695..4183
                     /locus_tag="ACG8WP_002592"
     CDS             3695..4183
                     /locus_tag="ACG8WP_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9028850.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4479..4760
                     /locus_tag="ACG8WP_002593"
     CDS             4479..4760
                     /locus_tag="ACG8WP_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028851.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4762..5178
                     /locus_tag="ACG8WP_002594"
     CDS             4762..5178
                     /locus_tag="ACG8WP_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028852.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2148 a          981 c          945 g         1697 t
ORIGIN      
        1 taacacaaag gcgtgattat tttgaaaccc aaaaagaaaa tttatctagg tctcctcttt
       61 tccttgatga ttggaatttt tagtatagga attacaaata cctatgcttc agaaaattct
      121 aatttcaata tcaactccca accaattgaa gaacaagcaa ttaattttgc gaaaactgat
      181 tttgaacact ccataacttc aattctttta atggaaaata acaccgaaac tgatattaac
      241 tatactcttg gttcgccatt taaaatgctc gatggagaaa atacggcaaa tagttctatt
      301 ttctttccag tattttcaga cgctgataat acgattaaat atgtttatac cgtcaaaaaa
      361 aatacaaatg gagaatattc aggaagtatt tcacaatttt tagcaaaaga acttcaaaat
      421 ctagtagata gtaatataac agatacaacc ttattttttc aagataatag catttattat
      481 tccgataatg gacaaattaa actactttgg aaatcacctt taagtacaga aaaaaatggt
      541 gtttcttccc ttacagaaaa aaatgatctt atatccgtaa atataaaaaa taaaattaaa
      601 aatttttcta aaaaacaaaa ttttcctgcg gttaaacgtg taaaaagtgt ctcctccgga
      661 gataacttta ttatgattaa ttggcgtata gcagaaaccc aaacaagttt tccgtggtgc
      721 gctgcttacg tagaagctgc tatcttatgt aacaaaacag atgtacgtgc tacttcagct
      781 agagccatta tgcattatac ctatcctaat ttaaacaaca gtcaaattga atcaaaaagt
      841 cttaatcaag atcaaataat aaaatatgca aattactgtg gtgtttatcc taaaaaaata
      901 gcacgaatac taaacctttc agaggtccaa acacaactaa gaaaaggaaa tgccatttat
      961 atgggaactg ctggttatgg aaattatagt aacagccgtc atgcacttgc attatttggg
     1021 tgggtcaatc aaggtggtta tcaaacttac tatgtgtgga atccttggta taactaccca
     1081 actgtaataa gtgcaaatgc aggaccagtt acaattcctg tacctggagg tggttttact
     1141 tgggacaata ccattacaaa ttggtaaata aactcattgc tttagttatg ggtctagtgt
     1201 tagttctttt cttaactagt tgttcaaaaa aacaagaaat acaaaagact gatctgattc
     1261 cttttctagg aacttggcaa actaaagatc attcagaacg tctagtaatc actaatttaa
     1321 atacttcaaa aaatatagaa caatatacca tcttagtaaa agagaacaat cataaaattc
     1381 aaatcattga taaaaattta aatataaatg aacaagtttt catttacaaa gcaacaaata
     1441 atttactaac actgcgctcg aataaaaaac aattagatta cacttttagt ttggattctc
     1501 caaaaaagct cgtatttatt ttgacaacga atgatgggac agaagggaca gctaaaccaa
     1561 ttgaattttt taaagctgat tgatgaatag ttgtttgata ttacaaaaca ataatttcac
     1621 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagc cttttttgta
     1681 ttgtggagcg tgggttttga gggggattca agggggagct tgcgcccctt tgacaagcgg
     1741 ttatttgcac caaatacgta ggtatttggc tagcaaatat agagcttgcc aagcccttta
     1801 gtgtgttttg tagaaaggga ccttatagtc actttcacat tacaaacgat acgtcctatg
     1861 gacgtatcaa aagggtggga cttatgagca aattaaagca aaatttggct agcaatcctt
     1921 ctaaaaaata cacctattga tcagagtcaa aataaatcag ttttcgagtg agcgaatcca
     1981 aatttgcaac tctgaagctt tgcaaatgag tgtgccggct tccgtaaaag tctaggtaac
     2041 attagtcgat atatacatag ggctctaatt tgcgttttaa ggcgttttaa acgttctggt
     2101 atgtaattat accaaagcga ctttaaaacg ccttaaaacg aattttagag gttctgtaga
     2161 acgttttagg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa aaacaagata
     2221 agggatttta tctagacttg tcttaaaacg ccgtagatga gcgctcagag cttctaaaag
     2281 gactccttac gaactgtttt taagcaaagg agcctttagg cgactgcgca gtttaatgtg
     2341 agagcgtagc gatcacacct ttaaagcgac cgtagggagc agattcccag tttaggaaat
     2401 ttcgcgaacc tgtttggaga aaaaaagtag gaaaatttta ggcgggaaat tttgcccccc
     2461 tcttttataa tctcttttat aacctctttt taaacctctt taccgtcggc tgtatcctta
     2521 ctctcccaag gtgtgcagaa tcttttaggg gtctttttgt ctgtttttag gggtcttttt
     2581 gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     2641 ctttaattag ggggttgacc cccattagaa agctctttat aattttgtta agtagttaat
     2701 cttgaaggag gattttatgg caaatgaaat tgtgaaatac caccatgaat taaatacgat
     2761 tcctttacgc aaatttacgt cagtagaaat gaatttattc ttctctataa tatctagaat
     2821 gcgtgatgta ggcgataaaa aagtacaatt tacattcgaa caattaaaag atttaagcaa
     2881 ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt atgacaaatt
     2941 aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac gatttgtttt
     3001 atttaatcaa tttaaaatcg atggaaaagc tgatattcct tttgctgaaa tacaagtcca
     3061 tgagaaagcc ttacccttac ttaataatct tgaagaatgg gttcgatatt ctctccaaca
     3121 attcaatgaa ttagaaagca gttattccaa aaccatgttt agattattaa aacaatacag
     3181 aaccaaagga attgccaatt tttcaaaaga agactttcat gaattacttg atatccctaa
     3241 aagttataga gaaagtgata taaataaaaa aattataaag cctattcgcc aagaactaac
     3301 cgcaatcttc aaaggtttga caatccaaaa gagatacgga aaaggacggg gaaagcctgt
     3361 cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact ttaacaaaca
     3421 tgctccaacc aaagaagaac aaaataaagc tttggaacta gctaatcaac aggttcaatc
     3481 tgaaaaagaa cctacttcat tacaagaaaa aatcaaagaa caaaaacgaa aattagaact
     3541 tttacaacag ttagcagaat tagaaagaaa acaaaatacc ccaagtaaga aaattaacaa
     3601 acaaatagca gataacaatt ccgatattac cccagaattt aaagaaaact tactagataa
     3661 tctccaaaac ttatttaaag aataaggggt ttttatgaca gataaaacta tcaaagaatt
     3721 agcagatatt ctaggtgttt caaaagataa agtgaagtat caactgagga aattacctag
     3781 taattttacc tacaaaaaag gaaatattac ctacctgaaa aaagaagcag taacaacaat
     3841 tacaaaggtt ttatcaggta atgaatcggg taacttacct agtgaattac ccgattcatt
     3901 acctaccgat tacctactca aggagattga aactaagaat aatcatattg aagaaaaaga
     3961 taggcaaatt gaaaaacttc aaatagcttt agaaacgcag caaaaattac tcgaccagca
     4021 acaacaactc accctacaag ccaatcaaca aatcaaaaaa cttcacgaac aactccagct
     4081 aacctatgca gaagaatctt cagaaaaaga acccctttct gttactgaag aaactcaaaa
     4141 aggacaaaac aaggagaaaa aatggtggca attctggcaa taacccaaca atttttattc
     4201 ataaaaattg ttgggttact ttcttttttt gcttttacaa gcatagcgac atgatctcac
     4261 gtcgctattt tttaaagagc ataaggaaac gcggtatcac tttcttacga aaggtgctag
     4321 gtaggctgtg cctaccacgt ttctaagaaa aaaatttgct atcacttttt cgtcattgtg
     4381 gatagcttat gtatagtgtt tcggcaaaaa acagctatac aaatactata tttgtatgat
     4441 atacataaat tatacacaaa tgcgaaagga attcttctat ggacatttta gttcggaata
     4501 ttgatccaaa agttatttca gaaatagata gacgttgcca agaactcaca acaaaaactg
     4561 gcaaaaaatg gagccgtaat gcgtacctaa aagttctaat cgaaaacgat tttgaccagg
     4621 cacttaggca ttacaaaaaa gatcaattcg atttattact cgaaaaattt atcgatatac
     4681 aagcaaaaaa tactgaaaca ttgaacgact atgttcatac aaacaaccaa ctcattgaaa
     4741 cacttatcgg aggaatataa catgagaaaa acttctgttc aaaaacacgt taccctttat
     4801 cccagtcaca ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     4861 tcggaagcca ttcgttttct atgtatgagt tatgaaccta atactaatac gacaactacc
     4921 agcaaccaag caaaactaaa tgcgatggga aaagaaattt ctattatgtc cgaaatcatt
     4981 gcagaatttg ctgatttata tctaaaagat actggtgttc ttatcggtac aaattctaaa
     5041 gtatatcaag aagtgaaaga aaaaattgaa aatcaactca atccctcccg acgaaatcaa
     5101 cccaaaaatc gtcaaaaaga aaccaatcaa acattcactt ctagaaattt tgacccaaca
     5161 aactttctaa aaagatgaaa aagaaaccga aaattttttc tatttctcag tttctttttt
     5221 agggggcagt cttccagcaa gatttgcaag ttaccggtaa cttgcacaaa aaaagaaaaa
     5281 tccgcttgtg cgaatccttc tttttgcatt ttggggcgct gccccaatct ccaaatatcg
     5341 gttatttaat agcgtggtaa ctcaaaaaaa tagaacgtac tctgaaataa aaaaagactg
     5401 ctctaacgag cagccaataa aaaataatga gcgtttattc ttgaaaaagc agcgcgctta
     5461 tgaagcgctg ccaaaaattt accatatttt atgaaaaata acaagtttct aaaaaggaat
     5521 cggttaagat tcacaaagaa tcgtaaacag acaatagcgg aaaatcaagc ttccgaacgt
     5581 accttttagc tagaccaatg aaaaatcaca aagtgagcgt ctctatccat tggtagaggg
     5641 gctacttggc atagtgtaca ttatgtctac tagctcgata aataacgatt atgttcccaa
     5701 aacaatattt tatgaactat aacaaaaatt tgttatcgca tattgtatat tttaattata
     5761 ggtgtatatc a
//
LOCUS       DAZKAY010000130         5569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000130 DAZKAY010000000
VERSION     DAZKAY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.135"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8WP_002595"
     CDS             complement(<1..300)
                     /locus_tag="ACG8WP_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9028853.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8WP_002596"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8WP_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8WP_002597"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8WP_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028854.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8WP_002598"
     CDS             1706..1942
                     /locus_tag="ACG8WP_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028855.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8WP_002599"
     CDS             1949..3901
                     /locus_tag="ACG8WP_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9028856.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8WP_002600"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8WP_002600"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9028857.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8WP_002601"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8WP_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9028858.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1812 a          971 c          902 g         1884 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattac
//
LOCUS       DAZKAY010000131         5513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000131 DAZKAY010000000
VERSION     DAZKAY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.136"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..948
                     /gene="purR"
                     /locus_tag="ACG8WP_002602"
     CDS             130..948
                     /gene="purR"
                     /locus_tag="ACG8WP_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9028859.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1451..2824
                     /gene="glmU"
                     /locus_tag="ACG8WP_002603"
     CDS             1451..2824
                     /gene="glmU"
                     /locus_tag="ACG8WP_002603"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9028860.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2918..3889
                     /locus_tag="ACG8WP_002604"
     CDS             2918..3889
                     /locus_tag="ACG8WP_002604"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9028861.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3895..3997
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4063..4632
                     /gene="thiT"
                     /locus_tag="ACG8WP_002605"
     CDS             4063..4632
                     /gene="thiT"
                     /locus_tag="ACG8WP_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9028862.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4713..5486)
                     /locus_tag="ACG8WP_002606"
     CDS             complement(4713..5486)
                     /locus_tag="ACG8WP_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028863.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1806 a          970 c         1164 g         1573 t
ORIGIN      
        1 ttttataggc ttctttttat tttaataaat accgaactat tttcttattt tttcataaaa
       61 tgctcgaatt tttgaagaaa aacttatata atgtaaaacg ataaaatttc ttaaaaggag
      121 ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg acatgacaca gtatttattg
      181 aatcacccgc atgaattgat ttccttaaca agctttgccg aacgttatga atctgctaaa
      241 tcttctatca gtgaagattt gggaatcatc aagaaaacat ttaaagaacg agggtatgga
      301 accttggaaa caatcccggg tgctgcaggt ggtgtattgt tcatccctga aatttcatat
      361 gaagaggcga aaaaatatat tgaatctcta gcagaacgtc tatcagaaca agaccgtctg
      421 cttccaggag gatatgttta tctctctgat ttgttaggag aacctgaact gctgcgtcaa
      481 gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa tcgatgctgt aatgactgta
      541 gccacaaaag gcgtacctat tgcccaagct gtgtcctatt atctgaatgc gccattcgtt
      601 attgtacgcc gggactcaaa aataacagaa ggatcaacag taagtgtcaa ctatgtgtct
      661 ggatcttctg aaagaattga aaagatggaa ctgtctaaga gaagtttgaa acgtggttca
      721 cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa cagtcaacgg aatgaagagc
      781 ttgattgaag aattcgaatc agaacttgtt ggcatcactg tatttgccga atcaaaattc
      841 aacggtcatc gcgccatcga tgattatacg tcactgcttt atgtcaaaga tgtagatact
      901 caaacgaaaa atattacggt tgtgccagga aattattttt cagaataaga ggttgtgaaa
      961 aagctgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acattttttc
     1021 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcta gaggttgtga
     1081 aatcagcgtc ctgtcctgag tattagctca ataaacggaa aaattgctcc tcatattttg
     1141 ccgtttattg agctaatacc gaaggtcagc tgattgaaaa acgtttaatg aagaggttgt
     1201 aagcctaccg tttaactcta agtcacaaga aacgatttca aaaaacagct tttcatgttt
     1261 tatgagtagt gtgacttgtg accgaggagt tggcaggaga gcaccggtta ccaaatagat
     1321 aggcccgtta tggtctatct atttttttat ccagaaaacg ctgggataaa agaaaatcac
     1381 ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa atcagcaaac tatatttagg
     1441 agtgacgcag ttggacgcac gttatgcaat tattttggcg gcagggaaag gtacccgcat
     1501 gaagtcaaaa ttatataaag ttttacatcc cgtttctggt aaacctatgg tagaacatat
     1561 cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt acgatcgttg gacacggagc
     1621 agaacaagtt aaagcacaac taggagaacg cagtaaatat gcgttacaag cagaacaatt
     1681 gggaacagga catgctgttt tgcaagctgc ttctttcttg gaaggaaaaa aaggaacaac
     1741 gttagttatc agtggcgata caccattatt gactactgaa acgctgaaca atctttttga
     1801 atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg gcacaagcag aaaacccaac
     1861 aggatatgga cgaatcatcc gtgaccatat tgggatcgta gaaaaaatcg tggaacaaaa
     1921 agatgcaaca ccagaagaag ctcttgtaca agaaatcaat acaggaactt actgttttga
     1981 caacgaagct ttgttcgatg cgttaagcaa agttggaaca aacaatgctc aaggtgaata
     2041 ctatctaaca gatatcatcg aaatcttaaa agaagaaggc catacagttg ctgcttatca
     2101 aacggatgat tttgaagaat caatgggagt caatgatcgg atcgcgttag ctaaagcaaa
     2161 tgagatcatg agaaagcgaa ttaaccagat gcatatggtc aatggcgtaa gctttgtcga
     2221 ttcagcaacg acatacatcg atgcaggcgt agaaatcggt ccagacacat tgatcgaagc
     2281 aggtgtacaa atccaaggaa atacagtcat tggttctgat tgtgtcatcg ggtcacattc
     2341 aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt gaaaactcag tcattgaaag
     2401 cagccatgtc aagaaacatg cagatgtggg accttatgca catttacgac caaaagctga
     2461 aattggcgaa aacgtccata tcggaaactt tgtggaagtg aaaaatgctc aaatcggcaa
     2521 aggaactaaa gtcggtcact tgacttatgt tggtgatgct acgctgggag aagaaatcaa
     2581 tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag aacaaacatc atacgactgt
     2641 cggcgaccac agctttattg gttctagcac gaatatcatc ggacctgtcg aggtagctaa
     2701 aaacagttcg attgctgccg ggtcaacaat tacagacaat attccagaat atgcgctggc
     2761 aattgcgaga gcaagacaag tcaataaaga aggatatgca aaaaaactcc cttatctaaa
     2821 ctaagacttt tgcaaaatca cttgatttat ttaaaaaata tgactactat ttattaggta
     2881 gaattctaga gttgtgaaaa agaaagtgga ggtccccatg tcaaaacatt actttgaccc
     2941 aagattaaaa atttttgcgt taaattccaa tcgcccatta gcagagaaaa ttgcagcagc
     3001 agtcggcgtc gagttaggaa aatcatctgt tactcaattt agtgatggag aaattcaagt
     3061 aaacattgaa gaaagtattc gtggatcgca tgtctatgtt attcaatcaa cgagcagccc
     3121 agtcaatgat aacttgatgg agttgctgat catgattgac gcactgaaac gagcaagtgc
     3181 aaaaacaatc aacgtagtca tgccttatta tggctatgcg cgtcaagacc gtaaagcacg
     3241 tgcacgggaa ccaatcacag cgaaattagt agcaaacatg attcaaaaag ctggtgcaac
     3301 aagaatgctg actttggacc ttcatgcagt tcaaatccaa ggtttctttg atattccagt
     3361 cgatcatttg atgggtgcgc cacttattgc caattatttc ttagaaaaag gaatcaaagg
     3421 cgatgatgtt gtcgttgttt caccagacca tggtggtgtc acacgggcac gtaaattagc
     3481 ggaatttttg aaatctccga ttgcgatcat agacaaacgc cgtccaaaag caaatgttgc
     3541 agaagtaatg aatatcattg gacaagtcaa aggcaaaact tgtgtactga tcgatgatat
     3601 gatcgataca gcaggtacga tcacattggc tgccaatgct ttgaaagaag ctggtgcaac
     3661 tagtgtgtat gcttcatgta cacatccagt attatccggc cctgctttac aaagaataga
     3721 agattcagct atcgagcgtc tagttgttac agactcgatc tatcttccag aagatcgtaa
     3781 aatcgacaaa atcgatgaag taagtgttgg cggactaatg ggagatgcaa tcaaacgtat
     3841 ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa aataaataat aaataaactc
     3901 acaaagggga gtccaattgg gctgagattg aatctatttc taaacccttc gtacctgtat
     3961 cggttatgcg agcgtaggaa ttgtgaataa acagctgttt gactattttc gaaagctgct
     4021 tcctcctttg tgactttttt attcaaagga ggaagttttt ttatgggaaa aaatagagtt
     4081 tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt tgtcgttgat tccgatccag
     4141 atcggcagca gtttttctat ttcattaggg cagattccat tgactctttt tgcgttaaga
     4201 agagggacaa aggcagggat gctggcaggt ttcatttggg gcatcctaca ttttccgttg
     4261 ggacaagttt attatttaag tgtcgttcaa gtgttgattg agtacccgat tgcctatact
     4321 tttgcaggcg cagcagggct catggcttca aatgtccaaa aaggctatgt agacgataat
     4381 acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga ttggcgcact ttccagatat
     4441 ttttggcatt ttattgcagg agtggttttc tggggagcgt atgcactatg ggggatgaat
     4501 ccttggctct tttctttcgt aatgaatggt gccagcgggt tgagtacagc tattgtaacg
     4561 atcatcgttt tattagggat ccaacaaacg actccttcga tttttgtgcc agataaacga
     4621 caggagctgt aaataaaaaa gaagcaagaa acttgagaca gaatcatgtc ctagtttctt
     4681 gtttcctttt tattttttga taagaaatag atctacttca atggcagata agaaagatcg
     4741 gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt agattaaagt agcgatgatt
     4801 tcctcttctc gataccgggt gccttttaat ttccaaatat aatcttcaat cacatagatt
     4861 gggatacgat atttaccatc gatcaaacca acttgatcaa aagaaaaaat gatcccttcc
     4921 tgcatcaaat tacgcatttc tttcaataat tccacactga ttttttcaac aggtgtttct
     4981 tttttgaaaa aagatttttt tacattgatc ttatctgaca agatcgaact taatttacta
     5041 gcaaaaatcc tataaaattc atctaaatag cgataataaa tccgattcag ctgatcatta
     5101 agatcgaagt atacaaaatt attctgtagt ttataaaaaa acggcgaatg aagatgtgtt
     5161 ttcatatgac caaaatataa taattctgaa atttctaaag gtgtcaattc tttcagcatc
     5221 gttaaatcag taaagtcgat ccacttcaat tcatcgaatc cacgcctgtt acgagaagag
     5281 gaaaagaaac gctgaatatt ttctgtacca cggattactt tcagccctgt atgggattca
     5341 tattctccag cagaagcaga tggatcaagc aagagcaagt tttgaggaaa atgcacgata
     5401 ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa cagcattact tgtggtatca
     5461 atatgtacat aaactgactc agtcatcatg ttcctcctct tatttcctct taa
//
LOCUS       DAZKAY010000132         5448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000132 DAZKAY010000000
VERSION     DAZKAY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.137"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8WP_002607"
     CDS             131..505
                     /locus_tag="ACG8WP_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9028864.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8WP_002608"
     CDS             complement(619..1248)
                     /locus_tag="ACG8WP_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9028865.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8WP_002609"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8WP_002609"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8WP_002610"
     tRNA            1668..1741
                     /locus_tag="ACG8WP_002610"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8WP_002611"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8WP_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028866.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8WP_002612"
     CDS             2338..3666
                     /locus_tag="ACG8WP_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9028867.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8WP_002613"
     CDS             3868..4701
                     /locus_tag="ACG8WP_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9028868.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..>5448
                     /locus_tag="ACG8WP_002614"
     CDS             4717..>5448
                     /locus_tag="ACG8WP_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9028869.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKN"
BASE COUNT         1837 a          839 c         1075 g         1697 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaat
//
LOCUS       DAZKAY010000133         5339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000133 DAZKAY010000000
VERSION     DAZKAY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.138"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..414)
                     /locus_tag="ACG8WP_002615"
     CDS             complement(19..414)
                     /locus_tag="ACG8WP_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028870.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="ACG8WP_002616"
     CDS             complement(404..616)
                     /locus_tag="ACG8WP_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028871.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="ACG8WP_002617"
     CDS             1688..2290
                     /locus_tag="ACG8WP_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFP9028872.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="ACG8WP_002618"
     CDS             2645..2881
                     /locus_tag="ACG8WP_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9028873.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="ACG8WP_002619"
     CDS             2878..3291
                     /locus_tag="ACG8WP_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9028874.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="ACG8WP_002620"
     CDS             3834..4760
                     /locus_tag="ACG8WP_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFP9028875.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="ACG8WP_002621"
     CDS             complement(4757..>5339)
                     /locus_tag="ACG8WP_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028876.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DAZKAY010000134         5249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000134 DAZKAY010000000
VERSION     DAZKAY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.139"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..299)
                     /locus_tag="ACG8WP_002622"
     CDS             complement(84..299)
                     /locus_tag="ACG8WP_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9028877.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            523..1323
                     /locus_tag="ACG8WP_002623"
     CDS             523..1323
                     /locus_tag="ACG8WP_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9028878.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1342..3210
                     /locus_tag="ACG8WP_002624"
     CDS             1342..3210
                     /locus_tag="ACG8WP_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9028879.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3203..3694
                     /locus_tag="ACG8WP_002625"
     CDS             3203..3694
                     /locus_tag="ACG8WP_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9028880.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3682..4236
                     /locus_tag="ACG8WP_002626"
     CDS             3682..4236
                     /locus_tag="ACG8WP_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9028881.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4254..5249
                     /locus_tag="ACG8WP_002627"
     CDS             4254..5249
                     /locus_tag="ACG8WP_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9028882.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1845 a          832 c         1074 g         1498 t
ORIGIN      
        1 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
       61 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
      121 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
      181 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
      241 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt
      301 agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat
      361 ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca
      421 ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct
      481 tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat
      541 attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc
      601 gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata
      661 cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa
      721 gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga
      781 ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca
      841 gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa
      901 gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa
      961 tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac
     1021 agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt
     1081 gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct
     1141 tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc
     1201 cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca
     1261 gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga
     1321 taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta
     1381 ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat
     1441 caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc
     1501 atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc
     1561 atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat
     1621 ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa
     1681 gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat
     1741 gattttgatg taaagattac aggcacacca aataaaggac taagaataat aggagatgag
     1801 ctagaattac gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca
     1861 gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt
     1921 aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt
     1981 aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa
     2041 ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa
     2101 aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa
     2161 gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat
     2221 ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg
     2281 ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa
     2341 aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg
     2401 caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt
     2461 cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa
     2521 ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata
     2581 acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt
     2641 acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt
     2701 tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg
     2761 tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat
     2821 ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa
     2881 aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct
     2941 cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa
     3001 ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg
     3061 acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa
     3121 atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc
     3181 aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc
     3241 agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata
     3301 tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc
     3361 aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat
     3421 gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat
     3481 ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc
     3541 tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca
     3601 accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa
     3661 agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg
     3721 ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc
     3781 ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg
     3841 aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta
     3901 tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac
     3961 tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc
     4021 ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc
     4081 ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac
     4141 tttattggtg gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc
     4201 gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg
     4261 gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga
     4321 caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag
     4381 ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg
     4441 atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc
     4501 ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac
     4561 aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa
     4621 tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat
     4681 atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa
     4741 ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag
     4801 atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga
     4861 tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg
     4921 cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg
     4981 gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag
     5041 gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat
     5101 gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag
     5161 gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt
     5221 gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKAY010000135         5087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000135 DAZKAY010000000
VERSION     DAZKAY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.140"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8WP_002628"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8WP_002628"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFP9028883.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8WP_002629"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8WP_002629"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFP9028884.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8WP_002630"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8WP_002630"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFP9028885.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8WP_002631"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8WP_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9028886.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DAZKAY010000136         5037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000136 DAZKAY010000000
VERSION     DAZKAY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.141"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG8WP_002632"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG8WP_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9028887.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACG8WP_002633"
     CDS             complement(2065..3063)
                     /locus_tag="ACG8WP_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9028888.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACG8WP_002634"
     CDS             complement(3289..3942)
                     /locus_tag="ACG8WP_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9028889.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG8WP_002635"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG8WP_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9028890.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1494 a         1036 c          766 g         1741 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa
     4921 aactgtacta ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg
     4981 ttttttattt ctttaatagt aatgttttct gtcattttaa ataatactgt tctttat
//
LOCUS       DAZKAY010000137         4978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000137 DAZKAY010000000
VERSION     DAZKAY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.142"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1125)
                     /locus_tag="ACG8WP_002636"
     CDS             complement(85..1125)
                     /locus_tag="ACG8WP_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9028891.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2291..2377
                     /locus_tag="ACG8WP_002637"
                     /pseudo
     CDS             2291..2377
                     /locus_tag="ACG8WP_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2931..3155
                     /locus_tag="ACG8WP_002638"
                     /pseudo
     CDS             2931..3155
                     /locus_tag="ACG8WP_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3415..3669
                     /locus_tag="ACG8WP_002639"
     CDS             3415..3669
                     /locus_tag="ACG8WP_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9028892.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            3670..3987
                     /locus_tag="ACG8WP_002640"
     CDS             3670..3987
                     /locus_tag="ACG8WP_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9028893.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            4546..>4978
                     /locus_tag="ACG8WP_002641"
     CDS             4546..>4978
                     /locus_tag="ACG8WP_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028894.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1745 a          708 c          772 g         1753 t
ORIGIN      
        1 acatttgtaa tataattaga ataataaaaa atttttaatt ttaatgctac tccatgaagc
       61 aacaatcaat ttgtcagtga aatttcactc taaccagtct ttcgtaatag tttttgggaa
      121 gctttcagaa ttcaaacgtt tttcacttga tactttagca gctattggtt tttcgataaa
      181 ccaatttttt acatagacaa aaatcaggtt ctggatattt tcaacttttt cggtttttaa
      241 tttttgatag gcttttaaca gtgtcgtgta taattcttgt tctgcattga cataataact
      301 atcgagttct tcaaaaatta ttgaaatacc actttctgtt tcggcttttt tctttgcatt
      361 atgaatagtc tggacagttt tttgagcttc actgtaactt gaagaaaatg tttttattaa
      421 atttaatact cgttcaggga taaacgtagc aatgctgtcg tttgtcataa ttgtctcaaa
      481 attgtctaac aacaattgat cttgtaggcg gtctttttca gtgtctatat tgtgtctatt
      541 agtgtctaat atattattat taagttctgg tatgattttc ataccctctg atttatcaac
      601 gttttggcac ggtatgattt tcataccctc ttgagcccca agggattcag cgtctgattt
      661 ttcgtcgagg gtgggtatga ttttcatacc ctctgattta tcaacgtttt ccctattctc
      721 tctatcaaat ttttcgagta agtagatatc attttctgag acttttaatt ctgctagata
      781 tagtctatta ggattatttt tccctaactg tttgttaaat cccatctgct tttgtaataa
      841 taaaccaaag gattccaact cttttttgat tgctatcgct ttttgttttt ggcagtttaa
      901 tacgctacat agttcatttg tcgtataagt aaaataaaca ttaccttctt catcgatttg
      961 ccgtttgctg attgcgatct ctaatcttgc ttttaaaagc atataggcaa tcttagttga
     1021 atctttcatt tttttgtatt cgtcagaaac aataaagact tttggaaatt gataaaataa
     1081 ttcgttgtaa actctatccg ccttaaaaaa attaaaatca gtcattgtag atcctctact
     1141 ttcacgttgt tttatttatg taaaattacc tactcttttc gaaaatagca aagataaata
     1201 aaaaatgcaa acgcattaaa actgtttttt aattaaatac tacctaaaat cagaaataaa
     1261 atgaattatg tactaccttt aaagtcatta agaactactt taaataaata ttatttataa
     1321 ttaaatacta ccagaagaac gtatgaataa aatttagttt taaaattaaa gataattaag
     1381 tactaccagt gtcaaaacaa aaaataaatt aagaactacc ttatgcatat aaaacatact
     1441 accattacta gtaatatata ctaccttatt tcagtagtat atattatatt cttcttataa
     1501 gacaaggctt taggctagaa aacaatagat tatatactac taaaaaatac aataagtact
     1561 accaatgtca gatatatata ataattatgt actaccatta aaattaactt atttaataag
     1621 atactactgt gttaatctga aaaaaattac tttaaagtag ttacatacta cctgttaaat
     1681 tagatactac cattaatact taaattagat actacctata tttatattaa gtactaccgt
     1741 tctaggaata agataataaa aaaaaactac caaattgcaa ttaaatacta cttaaaatgt
     1801 aaaaaataag aactacccaa attaggtagt tcttaataag agaatatcag ataaatttgt
     1861 acaagtcata aaaaaattaa tcttaaattt ttaaatatat atacctgtta attataaact
     1921 actttattta ggtagtacat aattaacaga taaaactaca tgcttgtagt ttgtctagct
     1981 aatgagttgc atatgattta tataaaggtg tgcagcataa cttagttgaa gttaagagaa
     2041 tagaataaat attttttgtt ttgctaataa ttaaacacta cctaaatatg gtagtgttta
     2101 attatataat attcattttg ttacaaataa tcgtattata aaagcataac aattgggata
     2161 actatttttt agattattta agtactactt aaattaggta gtacttaaat tttggtttaa
     2221 ggtagtaggt aattaagaaa taatatatat cttcttttat aatagaaggg gagaggccta
     2281 gttaaaaaca gcgacaatta ataacaatgt atttaatgct tcacaaaatc catttggtgg
     2341 acaatgcatt gttattagcg atgatctacc gttctaagta gttttttcta ttggttgcat
     2401 tcgtgactaa ttatggtata attatgagta acaaagaggt atttcattat atcatcaaac
     2461 cccatcagcg tgtaggctga tggggttttt tttacgtttc tatattatag aattaactgg
     2521 taggcttact gtcttttttg gcaattttgc aatcaagcca cttgcagatg tagtgtgcag
     2581 ttacctctgc aaatggcttg attgcaaaaa caaaatcaaa aaggtatttc attatatctc
     2641 ctcctcccct tgccagatta ggccggggta gcagacagca aaactattat aacataactg
     2701 aaatacctaa atgacatata cagatatgat aatttatgta ttgatttttc taacaaaagc
     2761 acatatttta atatatacta cctattttag gtagtatata tttttagata caatatcgat
     2821 cagttttgag gaaatgaaat tctagatatt ttttgctaca tagagttact ttcaatattt
     2881 tttaacgtaa ttaggcagaa gtcactcatc ctctataggt ttgtgatgaa attgctattg
     2941 atccatttaa gtaagagcaa aaaactggag gtttatcagg taattggtct aatggattta
     3001 agtatgcggt caccgtgtat agagttgctt atgagattca agaaaataaa gttattccag
     3061 tattgctata tggaacacat aacaattttt atgaattagc cgagaacact cgcaacttta
     3121 ttcgtgtaga tgaatcggct tcggttgagt tataacttta gctatatcaa tcaaccgacc
     3181 gacatatgtt aaatctatat ataataaaaa tacctactta aatggatgtt aacggaagtg
     3241 tatggtgagt agttcactcg tattttgata tcgtttgtat atgttttcag cgttctaagt
     3301 ggcttatttt gtccaataat aatatggagt ggaatccctt aagaaacacc gttggcaatt
     3361 gtcgttataa catgttataa tttatttatc aaaaactaaa agaagtgtga taacatggga
     3421 aaagaactaa aggttagaaa gatcggcaat agcgtgggag tgatccttcc aagctctctt
     3481 ggattaaaaa gcggagacac tatccaagcc aaacaagaag gcaacttatt catattagat
     3541 actactcaaa tagcaaaaga acacgatcgc aaattgattg aagaaagttt ccaagacttt
     3601 gagaaaggtt taacagtttc agaaattgaa atggtaaaag ctttcggaaa atacggctgg
     3661 agtgaataga tgaaagagta cacgctagtt tattctaaaa gtttccagac cagccttgaa
     3721 acgataatag aagaatggga aaatgagctt gctctttctc cagccagcat aaaaagattt
     3781 gtaggtgcga tccagaaggc tttagaacta accaaaaatt ttccaactat gcatgaggaa
     3841 gtttccgaaa tctatggaat ggatatccca acatatcgga tcgtgatcgg caaaagctat
     3901 gccttatttt atcggatcga ccatgaaaaa aaagagatac ttgtaggacg tattttccaa
     3961 tctaaacaaa tgaaactagg cttttaacaa taattaggca aaagtcgctc atcctctaca
     4021 ggtgggtgat taatgccgct cgatatggtt gttaaccgtc ggttactcga agattgatat
     4081 ataagactta gtaccttttt attttgctcc atgttatggc cgaaggtcta aagatacaat
     4141 cattagtcta gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg gtgaataaga
     4201 gacactgcta ttagtgaaaa aataagatat caagtacgct ctattcccaa aaatttcgtg
     4261 actttactca tgagaggttc aagtagtata tttttaatca aaattgacgt aacttttaat
     4321 aaatactacc taaataaggt agtatttatt tttgtgtttt aaaagaggaa gtccaatata
     4381 tctatgggaa acttcttaaa tattatgttg gtacatgaaa atatacatcc ctgctagact
     4441 atttacaaca ttttactttg tacgtattct tgttttaaaa ggaatgggat aaaatatagg
     4501 tacaataact atttgatgct aaatataatt aatggaggag agatagtgaa catacttaat
     4561 gaaaaaagat atgtatggtt tgctggtaaa aaatatatta ctactttcaa aagattatcc
     4621 gagttaatgc agcagactgt taaaagtgct atgcctgaag gatacaagtt taattttgtt
     4681 caatcttcag tatctactga aagtatctat ttttcaattc tttctgagag taggcttttt
     4741 cattttagaa taagttctca tgtaaagaaa actgatgttg atatatatac gttccgtaca
     4801 aataattata aaaatttaaa tgatttgagt agaagtatca gtgaatatct caaggggaat
     4861 agctatctca agattcgatt ttcacatata ttaattttac taccatattt gaatgagaat
     4921 ttcaccctaa ttcaagtaga taagtctttc attgcctcag gtaatcaagg aaatatga
//
LOCUS       DAZKAY010000138         4931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000138 DAZKAY010000000
VERSION     DAZKAY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.143"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..971
                     /locus_tag="ACG8WP_002642"
     CDS             84..971
                     /locus_tag="ACG8WP_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9028895.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="ACG8WP_002643"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="ACG8WP_002643"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9028896.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="ACG8WP_002644"
     CDS             2273..2719
                     /locus_tag="ACG8WP_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9028897.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="ACG8WP_002645"
     CDS             2992..4344
                     /locus_tag="ACG8WP_002645"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9028898.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="ACG8WP_002646"
     CDS             4515..4802
                     /locus_tag="ACG8WP_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9028899.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1394 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 accccttttt t
//
LOCUS       DAZKAY010000139         4833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000139 DAZKAY010000000
VERSION     DAZKAY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.144"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2410
                     /locus_tag="ACG8WP_002647"
     CDS             8..2410
                     /locus_tag="ACG8WP_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9028900.1"
                     /translation="MLSFSIILSYLIVDLKLCNRTVLSTQKWLNKKYSNVTGFDKVPE
                     NGRTGWPTIYGLIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQ
                     GAFWCKGINPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFV
                     LVPGGDSKIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATG
                     SYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKF
                     MVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYL
                     TGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAID
                     AAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCS
                     QIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSG
                     RDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYS
                     GLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQM
                     FLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQD
                     VDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLE
                     NDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK
                     LN"
     gene            2490..2885
                     /locus_tag="ACG8WP_002648"
     CDS             2490..2885
                     /locus_tag="ACG8WP_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028901.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3210..3827
                     /locus_tag="ACG8WP_002649"
     CDS             3210..3827
                     /locus_tag="ACG8WP_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028902.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3868..4167
                     /locus_tag="ACG8WP_002650"
     CDS             3868..4167
                     /locus_tag="ACG8WP_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9028903.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4201..4368
                     /locus_tag="ACG8WP_002651"
     CDS             4201..4368
                     /locus_tag="ACG8WP_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9028904.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4413..>4833)
                     /locus_tag="ACG8WP_002652"
     CDS             complement(4413..>4833)
                     /locus_tag="ACG8WP_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028905.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1647 a          675 c          956 g         1555 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gttttaagta ctcagaaatg gctaaacaag aaatacagta atgttacagg
      121 gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg gacttattga
      181 aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac ctaccactga
      241 aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta aaaatattgt
      301 atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct tcgatggggt
      361 atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag ggtttaatgg
      421 aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg atatgagtgc
      481 ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat ggttaaatgt
      541 aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa
      601 tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg aagcaacagg
      661 gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa
      721 ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag acggtacatt
      781 cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa aatttatggt
      841 attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat tgtctagcgc
      901 tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa ctagatctca
      961 aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta ctggtactgt
     1021 tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga ataatatttt
     1081 ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat tagcttattt
     1141 tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa
     1201 tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat
     1261 tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta
     1321 tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc
     1381 ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat
     1441 gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc
     1501 tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa
     1561 tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt
     1621 acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac
     1681 attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac
     1741 agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct
     1801 gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt
     1861 gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc
     1921 aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat
     1981 ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga
     2041 tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc
     2101 aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa
     2161 tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt
     2221 attagaaaat gacgaaacag ttgatatctt aggtgtaggg ataatttcat ttttcattga
     2281 taaaatagtt aataattcac ttgggacaga ttttacttat cttgatttta ttggagatga
     2341 ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa atagaagtaa
     2401 acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa atatatacga
     2461 taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata gaatggaata
     2521 atctgactca aaataataaa aataattctg gatgtggaat tatctttatg tttgttatag
     2581 ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt gcgaatactc
     2641 ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct tatgatccaa
     2701 gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag
     2761 ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat attgtaaagg
     2821 caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt gttgtaatag
     2881 aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa
     2941 aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa tggcagaccg
     3001 ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg ggagtgtaaa
     3061 cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt tatgtatggc
     3121 ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt tttgaaacaa
     3181 ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc cgggtcagta
     3241 gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga atccctgaag
     3301 aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa tatcaattaa
     3361 tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat agactaggca
     3421 gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag cagattgata
     3481 tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta gatgggttag
     3541 agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa gacgagcgga
     3601 aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat
     3661 tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct gactggaaag
     3721 caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga aatacctttt
     3781 atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag atataacagc
     3841 tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt cgtttttact
     3901 ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc tttaaacggc
     3961 cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca gtattctaag
     4021 tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg
     4081 ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat tagtcaacag
     4141 ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag gaaaattttt
     4201 atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa tcgaaatatt
     4261 gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac
     4321 ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa acgaaatagt
     4381 gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt ctttatttta
     4441 taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg aatgccttgc
     4501 tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt tgagttaata
     4561 gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa tactccttgc
     4621 cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag cagtgataaa
     4681 aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc taaaaactgt
     4741 gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt attttttata
     4801 tcttcataat ctcttcctaa acgatctagt gat
//
LOCUS       DAZKAY010000140         4811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000140 DAZKAY010000000
VERSION     DAZKAY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.145"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG8WP_002653"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG8WP_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9028906.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="ACG8WP_002654"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="ACG8WP_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="ACG8WP_002655"
     CDS             complement(3253..3771)
                     /locus_tag="ACG8WP_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9028907.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="ACG8WP_002656"
     CDS             4138..4386
                     /locus_tag="ACG8WP_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028908.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1581 a          786 c          730 g         1714 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta g
//
LOCUS       DAZKAY010000141         4785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000141 DAZKAY010000000
VERSION     DAZKAY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.147"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..908)
                     /locus_tag="ACG8WP_002657"
     CDS             complement(<1..908)
                     /locus_tag="ACG8WP_002657"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9028909.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNAL"
     gene            complement(982..2061)
                     /gene="uxuA"
                     /locus_tag="ACG8WP_002658"
     CDS             complement(982..2061)
                     /gene="uxuA"
                     /locus_tag="ACG8WP_002658"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9028910.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(2064..3695)
                     /locus_tag="ACG8WP_002659"
     CDS             complement(2064..3695)
                     /locus_tag="ACG8WP_002659"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9028911.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            3999..4670
                     /locus_tag="ACG8WP_002660"
     CDS             3999..4670
                     /locus_tag="ACG8WP_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9028912.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1540 a          929 c          668 g         1648 t
ORIGIN      
        1 cttaaagcat ttaaatgcat ttgcatcgtc caaccatatc ttgtatactc agacatcaaa
       61 tgtaattgaa tacctgtaac aaattgtgca atttctgata aatggaaatc cgtttttcct
      121 gctaaagctt ttattaacag attgtcgagt attttttcat ctgctatttc ataaaaataa
      181 gtattcaaac cgtgatctgc taatcgacag ccaatttgat gaaaatgatc cactcgttgg
      241 ctcaatgctt gcttcaaccc attaaagtct ttgattggta tagccgctgt ttgactaagt
      301 tttaaaatat agttcgaaaa gcctgatgat ttgatactcc agaccttatc tggacggaaa
      361 gtaggtaata ccttgaaaga attatcctgt ttagaaagct cagtatgata ccacaagtca
      421 tctgtaggat catccgttgt acagatggct ttcacatcca tcatctccaa caattctttc
      481 gggcgatagg aatccgtttg aattttttcg ttagcacgtc gccaaatttc tggtgcattc
      541 ttctcagaaa ttgtcaaatc aatatcaaag taacgttgta attccaaatg agaccattca
      601 aaaataggat tacctggcgc cttctccact gtttttacaa aagctaaaaa tttttcataa
      661 tcatcacctg gaccactgat caactcttct tctataccat tagctcgcat caatcgccat
      721 ttataatgat ctccagctcc tttatcattt aaccatacct gagtcaaatt cttgtattgc
      781 ttattttcta gcaaatatgc tgggtctaaa tgacaatgat agtcgataat tggttggtgt
      841 tttgcaaatt tatgaaaaag tcttttagct atttcattct ctaataaaaa gttcttatct
      901 aaaaacatat ttaaacactc ctttccttcc gttagttttt agaagagaaa atttttcctc
      961 taaataaact cccttatata attatttact cgcccccttt tcaagcgctt cccaaatacc
     1021 atttaaataa actgcaccta gagctcggtc atataatcca taaccaggtc taccggtttc
     1081 tccccaaatc atccgaccat gatctggacg aatatagcca ttaaatccat tatcgtacat
     1141 tgtttttaat atcttataca tatctagaga accatattct gatttatgcg gcgcttcgta
     1201 aaaatcgagt ccattgtcac ttaaatattt gatattacga acatgtgcaa aaggaatccg
     1261 atcccgttga caaaattcag atagaatttc atagacatca ttatctggtc gttctgcaat
     1321 acttccagta cataatgtaa taccattaga aggagaatcc actacattac acaaccaatc
     1381 taaatcgtca cgatttttaa caatacgagg taatccgaaa atggaatatg gtggatcatc
     1441 tgggtgaact gccattttaa tcccaacttc ttcacatgtc gggataattg ctttgagaaa
     1501 atatgcaaag ttctctcgta atttctcttc atcaacttct ttataggctt ctaataattg
     1561 cttaatttgt gataaccgat gaggttccca acccggcaat tcaaaattat tagtagaatt
     1621 ttctacttct ctaataattt cctccggatc agtagggata ttttttgaaa tataggctaa
     1681 cgtagaagac ccatcatcta aacgataatc caaatccgat tttacccaat caaatactgg
     1741 cataaaatta taacagataa cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt
     1801 ataattctca atgtattgat ctcgtgttgg taaaccaatt ttgatatcat catgaacatt
     1861 aacactctca ataactttca aggtaagtcc attggcttcg acttcatctt ttaaagattt
     1921 aattcgttct ctcggccaaa cctctccggc tggtatatcc attaatgttc caacaattcc
     1981 tttcattgct gggatctgac gaatttcttg tagtgagatt ttttcttcat ttgccccata
     2041 ccatctaaaa gtcatttcca tagttacttt cctccaactt ctgttatata tttattgatt
     2101 gttttacgaa ctgcttgagg acctactagc atttcgcaaa agattttttc agttttcttt
     2161 cctaccccaa cttgatataa atctaaacca aagatttctt tattttctaa aatttcctgt
     2221 accgcacaat gaatctttga aggatcttgt tcacctaatt tcaatactga taatttatcc
     2281 tgcaactcta ccaataacgg atcaggactt ggcacaaaat tttccccttg atcatcgact
     2341 gccagtaaat aacgtaacca agcagcaata gcgataggaa taaatgttag attttctgca
     2401 tttccgtctt tttcgacata ctttttaatt gtttcaccat agcgaatcgc tactttttga
     2461 gaagtatctg ttgcaattcg ttgtggtgta tctggaatat agggattagg caaacgttta
     2521 ttaattactt catcaataaa ttgttttgga tcaataatgc ctggattttc aactactggt
     2581 aaaccttcta aataacctat tcctttgatt aaaccaacca aatcatcatc ctgcatctct
     2641 gctgaaattg attgatatcc tagcagacaa cctgttactg ccaaagcagt atgaagtgga
     2701 ttcaagcttg tcgtaacctt catgatgtct gctttatcaa ccgtctctct atctgtcata
     2761 atcacaccag ccttttccaa tttaggccgg ccatttggaa aactatcttc aataactaaa
     2821 taatgaacta cctcagtatt agtgaaagca gcaatattgg tatgtttttt tgtgtgaatg
     2881 atatctaaat cctcgagccc catcatcaat agatcttcct ttacattctc tgaaggattt
     2941 ggcgtaattc gatcaatcat actccaagga aaagtcactt tttctttatc ctttaaataa
     3001 gcaataaatt tttcagaaac atatccttct ttttgccaag cttctgcaat cgtaaaaata
     3061 ctatcttgaa aacgttgacc attctgtgag aaattatcag tagaaaccat cgcaatcggc
     3121 aactctccag cattaaatct gctatataac aaggatgtta caatactcat cgtatgttga
     3181 ggattgctag gtccagattt gatatcagct ttgactaccg aaaagaattg ccctttagaa
     3241 tcttttaaac tgtaaccttt ttcagtaata gtaaacgttg taaactgtaa tttaggatct
     3301 tcaaaatatt taaccactct ttgatagtcc gctatattct tcggattgca atataaacta
     3361 tcagccgtta ctgccaaaag tctttttttg agttcaccat ctccactcat aacaatttcc
     3421 aaaatgtcat tgttatagtc atgataagct ttttccacta cttcttcatc aaatgtttca
     3481 cacacaacta ctcctgtttc taattcattt tgatctgcta agcattgagc aatttctgca
     3541 tgaaatcctc gataaagatt tcctccacca aaatgaagcc atactggatg ttgctggcct
     3601 tcttgcttaa cttctttgac atcaaaacac ggcagtagaa tctgcatttc attgaattct
     3661 tcgatagaat tttgccaatt tttaatcgaa accataatta acctcctcaa actgatatgt
     3721 tagttttgat acattttata ttttaaaacc cattgtaacc gttgtcaata taaaaaggga
     3781 ataaacttgc ttttttctac catatcagaa tgattgtatt ttttattcag aaaaataaaa
     3841 tactccttct ttttggactg attttatcga aacttatttt ttcttttttt catgtattct
     3901 tttattttta taactagtgt attacttcaa aatttgatat tatatggcat gaacagaatt
     3961 aaaatatcag ttaaaataaa agttaaggag cattgtgtat gacaattaaa actagtctcc
     4021 aaaatcaagc ttaccaatct attcgccgac aaattatcta tgctgactta gaacctggaa
     4081 aaaaagtatc agaaaaaaaa ttagaagaaa cactttctat tggtcgtaca ccaatacgtg
     4141 aagcactgat tcaactccga caacaaggtc ttgttgatac aattcctcaa agcggaaatt
     4201 atatctcatt aattgattta gattcggcca aaaatgctcg atttgtcaga gaacatttgg
     4261 aacgacaaat tatgttagag tgttgtgcca aaatgaataa ccaaaaaaaa cggattttag
     4321 aaactataat ttcagaacaa gaaaaagctg ttagtcaaaa aaatgctcgt agtttttttc
     4381 ataatgataa tttattccat aaagcttgtt ttgaaattgc agagcgagaa gaagtatggc
     4441 aatggttaga aaataacaat acacacttag aacgatttcg atggctacga gtaaaaacgg
     4501 aaggattaaa gtgggaaaaa attatggaac aacattatca actatttgag gcattaacaa
     4561 aacaagatcc agaagaagct aactttctaa catcgttgca tcttcattta atgcttaatg
     4621 agcaaaatat tgttttagct actcatccaa aatatttcaa aaatatataa cttttaactc
     4681 tttttaaatt aaatggttgt tctgatcaga aaaatatcgc ttgttttctt attatgtaat
     4741 atatttcata aactattctg cataacaact attgtttgta ctttt
//
LOCUS       DAZKAY010000142         4766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000142 DAZKAY010000000
VERSION     DAZKAY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.148"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8WP_002661"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8WP_002661"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9028913.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8WP_002662"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8WP_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028914.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8WP_002663"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8WP_002663"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9028915.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8WP_002664"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8WP_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028916.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8WP_002665"
     tRNA            3917..4000
                     /locus_tag="ACG8WP_002665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4766)
                     /locus_tag="ACG8WP_002666"
     CDS             complement(4240..>4766)
                     /locus_tag="ACG8WP_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9028917.1"
                     /translation="KCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWS
                     SSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMI
                     MGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLT
                     VLGICTFIITVINR"
BASE COUNT         1438 a         1001 c          768 g         1559 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcc
//
LOCUS       DAZKAY010000143         4731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000143 DAZKAY010000000
VERSION     DAZKAY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.149"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1013)
                     /locus_tag="ACG8WP_002667"
     CDS             complement(15..1013)
                     /locus_tag="ACG8WP_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9028918.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1088..1384)
                     /locus_tag="ACG8WP_002668"
     CDS             complement(1088..1384)
                     /locus_tag="ACG8WP_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028919.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1377..1670)
                     /locus_tag="ACG8WP_002669"
     CDS             complement(1377..1670)
                     /locus_tag="ACG8WP_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP9028920.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1816..2367)
                     /locus_tag="ACG8WP_002670"
     CDS             complement(1816..2367)
                     /locus_tag="ACG8WP_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP9028921.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2541..2840)
                     /locus_tag="ACG8WP_002671"
     CDS             complement(2541..2840)
                     /locus_tag="ACG8WP_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFP9028922.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2867..3142)
                     /locus_tag="ACG8WP_002672"
                     /pseudo
     CDS             complement(2867..3142)
                     /locus_tag="ACG8WP_002672"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            3480..4163
                     /locus_tag="ACG8WP_002673"
     CDS             3480..4163
                     /locus_tag="ACG8WP_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9028923.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4200..4697)
                     /locus_tag="ACG8WP_002674"
     CDS             complement(4200..4697)
                     /locus_tag="ACG8WP_002674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9028924.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1402 a          906 c          693 g         1730 t
ORIGIN      
        1 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
       61 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      121 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      181 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      241 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      301 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      361 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      421 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      481 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      541 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      601 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      661 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      721 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      781 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      841 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      901 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
      961 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1021 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1081 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1141 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1201 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1261 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1321 gcatatattg atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1381 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1441 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1501 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1561 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1621 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1681 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1741 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1801 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1861 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1921 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     1981 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2041 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2101 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2161 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2221 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2281 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2341 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2401 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2461 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2521 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2581 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2641 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2701 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2761 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2821 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2881 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     2941 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3001 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3061 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3121 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3181 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3241 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3301 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3361 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3421 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3481 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3541 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3601 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3661 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3721 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3781 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3841 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3901 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     3961 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4021 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4081 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4141 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4201 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4261 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4321 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4381 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4441 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4501 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4561 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4621 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4681 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DAZKAY010000144         4723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000144 DAZKAY010000000
VERSION     DAZKAY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.150"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8WP_002675"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8WP_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9028925.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8WP_002676"
     CDS             724..1878
                     /locus_tag="ACG8WP_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9028926.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8WP_002677"
     CDS             2093..3061
                     /locus_tag="ACG8WP_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9028927.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8WP_002678"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8WP_002678"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9028928.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8WP_002679"
     CDS             4091..4699
                     /locus_tag="ACG8WP_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9028929.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DAZKAY010000145         4695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000145 DAZKAY010000000
VERSION     DAZKAY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.151"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG8WP_002680"
     CDS             complement(12..482)
                     /locus_tag="ACG8WP_002680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9028930.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG8WP_002681"
     CDS             complement(641..1099)
                     /locus_tag="ACG8WP_002681"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9028931.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG8WP_002682"
     CDS             complement(1270..2415)
                     /locus_tag="ACG8WP_002682"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9028932.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG8WP_002683"
     CDS             complement(2428..3345)
                     /locus_tag="ACG8WP_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9028933.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG8WP_002684"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG8WP_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9028934.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACG8WP_002685"
     CDS             4437..4607
                     /locus_tag="ACG8WP_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028935.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1463 a          850 c          881 g         1501 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaa
//
LOCUS       DAZKAY010000146         4635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000146 DAZKAY010000000
VERSION     DAZKAY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.152"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG8WP_002686"
     CDS             174..518
                     /locus_tag="ACG8WP_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9028936.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG8WP_002687"
     CDS             519..938
                     /locus_tag="ACG8WP_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9028937.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG8WP_002688"
     CDS             complement(1030..1380)
                     /locus_tag="ACG8WP_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028938.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="ACG8WP_002689"
     CDS             complement(1346..2677)
                     /locus_tag="ACG8WP_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP9028939.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="ACG8WP_002690"
     CDS             2933..3499
                     /locus_tag="ACG8WP_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9028940.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACG8WP_002691"
     tRNA            3644..3731
                     /locus_tag="ACG8WP_002691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="ACG8WP_002692"
     CDS             4065..>4635
                     /locus_tag="ACG8WP_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9028941.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAZKAY010000147         4592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000147 DAZKAY010000000
VERSION     DAZKAY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.153"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..2857
                     /locus_tag="ACG8WP_002693"
     CDS             188..2857
                     /locus_tag="ACG8WP_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9028942.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2948..3562)
                     /locus_tag="ACG8WP_002694"
     CDS             complement(2948..3562)
                     /locus_tag="ACG8WP_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9028943.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3682..4221
                     /locus_tag="ACG8WP_002695"
     CDS             3682..4221
                     /locus_tag="ACG8WP_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028944.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4218..4556
                     /locus_tag="ACG8WP_002696"
     CDS             4218..4556
                     /locus_tag="ACG8WP_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFP9028945.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1422 a          790 c          860 g         1520 t
ORIGIN      
        1 aaatttttaa gggtgggact tttgattcct accctttttt tgcgtttatt tttgttatac
       61 gaacaaatta tgaagttatt tattctaaat tttatttttt tgtgaaaatt agactctttt
      121 taagcagaga aaaacaagga tatgataaac tgaatacaac ttcatagaaa tttggtggaa
      181 aataaatatg aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc
      241 tagtttcctg attccgtttt tgattttagc acttgtttat ctaagtatcg ggatctatcc
      301 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
      361 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
      421 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
      481 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
      541 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
      601 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
      661 catcatgtgg ctggatgcat ttgtttatct tccattagtg attttgggga ttcatcgtgt
      721 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
      781 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
      841 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
      901 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
      961 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
     1021 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
     1081 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
     1141 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagtttct atcttgttcc
     1201 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
     1261 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     1321 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     1381 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     1441 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     1501 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     1561 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     1621 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     1681 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     1741 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     1801 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctaatgga
     1861 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     1921 cttccgccaa gctggtcaaa gtggcgacta tacactgtat cagaatgtca atgcattgcc
     1981 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     2041 gaccagtcag acaaacttga tcaatacatt gtctggttta aatgaacagt attttacatt
     2101 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     2161 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     2221 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcacaat tagaaagttc
     2281 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     2341 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     2401 tggaacgaaa gaagtaagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     2461 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     2521 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     2581 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     2641 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     2701 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     2761 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaaa
     2821 accgcgaaat acacacagaa gaagacgaag aaaataaaga aacgatggaa tctgtgtcac
     2881 tcttctattt aaatggcaaa aatttcaatc tttaaatgac aaaaaccacc tgatttttgg
     2941 tggtttttta tatacctatg actaaactat ctcttgctgc ttcgacaact tctacagtga
     3001 tcttatccat ttgattgatt cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa
     3061 gcctaaaatt tccttttgtg atcttaatga tagttgtgat agcctcataa tcagaaaaat
     3121 cttctagctt gatatcaatt cctaaatctg cccatttgta ttccaaaata tgatgtgttt
     3181 cgtccttact aaggttatca aattcatgtg caaatcctat gcgagagtaa agctgcggat
     3241 aacgagacaa tcttttttca ataccaggca taccgataag aaccattgcc agatcatttc
     3301 tatcgtaaat atctcttagc tgttctaaat gctgaagctt taaacgatca atttcatcga
     3361 caataattaa gtcaattgct tcataggttt taggcacagg aggatcttct tgattgtatt
     3421 ttagtaccat atgcattgct ttagtcagac ctattctact agttattgtg ttaatatccg
     3481 aactgacacg agacatcttt tcagctggta cagtataaaa aactttattt acacttaata
     3541 tttcatctgt tgcatttatc aagaacaaga aaaagtctga atattcaaaa aaaataacgg
     3601 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc tcaaaattta
     3661 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
     3721 ggctttattg gtattgggac ttctggaaat atagttaaag ctagcacgat ggttgaacaa
     3781 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
     3841 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
     3901 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
     3961 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
     4021 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
     4081 tatggttatg gtggaggaat gtattttaca tttactggga aaagacaata ttacagtgta
     4141 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
     4201 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
     4261 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
     4321 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
     4381 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
     4441 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
     4501 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
     4561 aatatgcaca gttgagaacg gaatttgaca tt
//
LOCUS       DAZKAY010000148         4524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000148 DAZKAY010000000
VERSION     DAZKAY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.154"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..314
                     /locus_tag="ACG8WP_002697"
     CDS             <1..314
                     /locus_tag="ACG8WP_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028946.1"
                     /translation="LQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYSPISQVL
                     DLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIIIYKKNSFKYFKENI
                     L"
     gene            311..931
                     /locus_tag="ACG8WP_002698"
     CDS             311..931
                     /locus_tag="ACG8WP_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9028947.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1045..1182
                     /locus_tag="ACG8WP_002699"
     CDS             1045..1182
                     /locus_tag="ACG8WP_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028948.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            1624..2004
                     /gene="mobC"
                     /locus_tag="ACG8WP_002700"
     CDS             1624..2004
                     /gene="mobC"
                     /locus_tag="ACG8WP_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP9028949.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1986..2900
                     /locus_tag="ACG8WP_002701"
     CDS             1986..2900
                     /locus_tag="ACG8WP_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9028950.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2860..3138
                     /locus_tag="ACG8WP_002702"
     CDS             2860..3138
                     /locus_tag="ACG8WP_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028951.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            3140..3829
                     /locus_tag="ACG8WP_002703"
     CDS             3140..3829
                     /locus_tag="ACG8WP_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028952.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3950..4036)
                     /locus_tag="ACG8WP_002704"
     CDS             complement(3950..4036)
                     /locus_tag="ACG8WP_002704"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9028953.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         1692 a          749 c          798 g         1285 t
ORIGIN      
        1 atctacaata tcttttaata ttaggattat ttataataat taattttcaa atgatttttg
       61 ttagttttac tataaattca aaaaaaatag ctttaaaaaa aatatttgga tattcaccaa
      121 taagtcaagt tttagattta tgtttactcc cttttacaat agagttattt gctactatgt
      181 gttttcaatt gatattttca aaaaacactt acttgtattt tataattttt atgatctttt
      241 taataaatct aattacttct attattattt ataaaaaaaa tagttttaaa tattttaagg
      301 agaatatttt atgatattat cagttgagaa tttcaccctg tcaattaata aaaaaaagat
      361 caaacaaaat tggaactttt caatatcaga aaaagatttt attatagtaa caggaaaaag
      421 tggtagtgga aaaactactc tgttaaataa tttaagccta atagataaac aatataccgg
      481 taacttattc tattttgaaa agaaagtttc taaacatgat atacaacgtc tacgaagaaa
      541 tcatattagc tatttatttc aaaactatgc tcttcttgaa aatcaaacag tacaatacaa
      601 tttcgaatta gcaaaaaagt ttaacagtaa tatttccaat gatcatattt ataatttatt
      661 aacagatttt tctttgccaa aagatatctt aaaagaaaaa atctttctct taagcggtgg
      721 agaacaacaa agaatagcat taattagaaa tatgttaaaa ccatttgata tattatttgc
      781 agatgaacct acaggaaatt tagataaaga aaactcaact tttataatga actttctgaa
      841 aaatttaaca gaaaaagaaa aaaaagccgt tgtactagta acacatgact taaatgttct
      901 aaactatgga actaatatta tagaattata atttttaccc gtgatgctag ctgatttaaa
      961 actagcacca cgggtattta ttttatattc catctctcct atcccaaaat ttaaataatc
     1021 acttaataaa aaggaggcac cctagtgatt ttattgaaaa aagagtatga aaaattttat
     1081 gatagactta tttattcttt gcaattgcca aaaaaattaa accgtcaatt acaaaagact
     1141 tatgaaacta aatcaactga agaatgggag agttattgct agatttttgg tagaagtaac
     1201 aaaaagtgga catattatca taatgactcg ccgaaagtct gataaaaaat tggctaaaaa
     1261 tattgctgaa tgtttaataa aactatcagt tttagctaaa tagttagtag tttacacaaa
     1321 aaagcattta tgttttttgt tgcaaagtaa caatttcatc ttcggtgaaa taagcgaaac
     1381 tttacaaaaa gtggctagca tttgctagct ttttttgtat tgtggagcgt ggcttttgag
     1441 ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcact aaatacgtag
     1501 gtatttggct agcaaatata gagcttgcca aaccctttta gtgagcttta ctcattgtac
     1561 ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga
     1621 cttatgagcg aacaaaatca aaatttggct agcaattctt ctaaaaaata tacctatcga
     1681 tccgagccaa aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa gctgaagcaa
     1741 tcggctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg
     1801 cgtctggtga caccgaaagt ggcgccagac attgcccagg caatcgcgcg cgatttagcc
     1861 aaagctggag ggaatatcaa tcaaattgct aagtggtgca atacacacca acatgacgtt
     1921 gcttctggtg acgctcaacg tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa
     1981 aagatatggc aacaattaag gtaagccgaa ccacttcttg tagtcgtgca attaattacg
     2041 cagaaccacg agcaaccatt aaaacaggaa taaactgtga cgtcgattat gccaaaagtg
     2101 aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg tgtacaggca cacttattga
     2161 ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc taatcaacta ggggcagagt
     2221 atgcaaaaaa aatagccccc gatcaccaaa cagctattta cacacataca gacaaagacc
     2281 atattcataa tcacattgtc attaactccg ttaatctgga gactggaaaa aaatttcaag
     2341 ctcatggtca agcatttcta aacaaatgtt atgacctaaa tgacgagatt tgtcttgcac
     2401 ataacttacc aatcactgag cgaggaaaga aaccagaaaa acgaaccatg tctgaaataa
     2461 aattaaaaga aaaaaatgaa cctgtttgga aagacgaaat tcggattgcg atcgatcaaa
     2521 caatgaaaaa caaaaaaacc agaacctatg accaattttg taattccctc aaaacattgg
     2581 ggattcattg cttcaatcgc gggaaaaatt tcacttacga attgatccac aaaaagaaaa
     2641 aagtacgatc caacagacta ggaaaggact acgaaaagga gacgatttta catgagctgg
     2701 acagacgaga acgaacctat aaccaacagc gaaccaacga gctacgaaga gctcgagcta
     2761 gcattcgcga aactgcagaa gaagtacgaa gagaacaacg aactcttacg cctgaacaac
     2821 cggaaattag accggctcga agaaaccatg aacaacacgt tgaaagagga acagaccacc
     2881 aacaagaatt atcgctctaa aacgttaaaa gacatagaaa acgaaataaa gaaacaacgt
     2941 gcttttctcg ttccttatac gcaagaaagg atacaaacta cgattgattt acaagttaaa
     3001 aaactccatc aagagctatt agaccagcga aataaggaac agaaaaaggt gcttattcaa
     3061 cgatcattgg attttcttgc ccccttatgt tgcttagtcc tgacactgtt cgcattttgg
     3121 ttagtatggg gaggctaaaa tggcatttta tttaccctat ctactcattt ttgttagtat
     3181 ttcaggaagt atttggctca tctataaaat ttttcaaaca agatattcat taaaaggctc
     3241 taaaattcga tttaagcgat ttttctttct tggctgtata ttttccttga taatcgtttc
     3301 aagcggctta ttcggcgttt tagagggaaa taagcgcgtt tctagaagta ttttattagg
     3361 aaatgtcact caaaagtatg aatcagcgcg tgataaaaag aaaaaagagc aagcattagc
     3421 acaaaaaatg gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca
     3481 agaaaaacgt ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc
     3541 agaaaaacag caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata
     3601 tgtaaaagat acaaagatag aagaagcctg tagtgatcta tttggagaca caaacccttg
     3661 gtttgccagt gaagaagaaa aaaaagaaaa acaacaatct gtcacatatg aacgctatga
     3721 aatcttactt caacaagcaa ctaacatgca aaatccgaca aaaaaagaaa ccgccttaaa
     3781 ctatctaaaa agtgtcaagg aatggttaga tcagcaacaa caaaattgaa tttgatttaa
     3841 aatcatgtta aactgaatgt gcaaatgatc aaacattaga tacacgaaag ccccagagag
     3901 gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt
     3961 ttcggttatt caaccaataa ctgaataaag agacacaaac tcctacaaga agggggcaaa
     4021 tgaacagttc aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
     4081 agttattata tcttaaaaaa ttaaatttgt gtggtgtttt taagtatcac ttgttttagg
     4141 gttgttaggg cttgccctga ccgcctgtaa ggcgcttctc gactagatta agggaaatta
     4201 atcaagaatt gtcttagaac ggcttagaca agctttcaga gtctcacacc tcctctttga
     4261 acaacggttt tagcaaagga gccggaggcg actgcgcagt ataatatgag agcatagcga
     4321 tcacaccttt ttagcgaccg tagggagcaa gttttccagt ttagaaaaat tcgcgaacct
     4381 ttttggagga aaaagtagga gttttttagg cgggaaattc aatgtgctct taagatatac
     4441 tgttagatat actgttagat atactgttaa taataactgt tagggattag caaccttatg
     4501 ggagagtaag tattgacata ggta
//
LOCUS       DAZKAY010000149         4461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000149 DAZKAY010000000
VERSION     DAZKAY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.155"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACG8WP_002705"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACG8WP_002705"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFP9028954.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACG8WP_002706"
     CDS             2298..3221
                     /locus_tag="ACG8WP_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9028955.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACG8WP_002707"
     CDS             3237..3776
                     /locus_tag="ACG8WP_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9028956.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAZKAY010000150         4450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000150 DAZKAY010000000
VERSION     DAZKAY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.156"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8WP_002708"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8WP_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9028957.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKRAQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG8WP_002709"
     CDS             complement(2017..2919)
                     /locus_tag="ACG8WP_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9028958.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG8WP_002710"
     CDS             complement(2916..3608)
                     /locus_tag="ACG8WP_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9028959.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG8WP_002711"
     CDS             complement(3651..4292)
                     /locus_tag="ACG8WP_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9028960.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          949 c          768 g         1357 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtgc acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc
//
LOCUS       DAZKAY010000151         4188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000151 DAZKAY010000000
VERSION     DAZKAY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.158"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG8WP_002712"
     CDS             complement(<1..506)
                     /locus_tag="ACG8WP_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9028961.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            complement(914..1618)
                     /locus_tag="ACG8WP_002713"
     CDS             complement(914..1618)
                     /locus_tag="ACG8WP_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028962.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1805..2050)
                     /locus_tag="ACG8WP_002714"
     CDS             complement(1805..2050)
                     /locus_tag="ACG8WP_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028963.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(2067..2918)
                     /locus_tag="ACG8WP_002715"
     CDS             complement(2067..2918)
                     /locus_tag="ACG8WP_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9028964.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            3084..4088
                     /locus_tag="ACG8WP_002716"
     CDS             3084..4088
                     /locus_tag="ACG8WP_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9028965.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1282 a          735 c          810 g         1361 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgctt ttttctctat
      661 cagaaaatat gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca
      721 ggcaactcca agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt
      781 gatttgttgg acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac
      841 ttgctgtaga aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg
      901 acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa
      961 tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt
     1021 cagtagtcat agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa
     1081 ctacttcatg ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa
     1141 aatcagcaaa acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg
     1201 cttttggttt ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg
     1261 caacagcttg cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga
     1321 tttcaataag aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc
     1381 tctttaatac agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg
     1441 ttttcccagc taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta
     1501 acggcaaacc ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta
     1561 caccatccaa tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat
     1621 gttctctcct tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag
     1681 taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt
     1741 tttttgaaag aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt
     1801 ctttctattt tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat
     1861 gttcgtgcca gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga
     1921 tttgatcatg tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa
     1981 agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga
     2041 ttggatccat aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg
     2101 ccgactacac catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca
     2161 aataattcag tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat
     2221 tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg
     2281 atttgtttca tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg
     2341 aggatcatat catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca
     2401 aggaaaactt gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg
     2461 tggggtaaga aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca
     2521 aatgctcgcc atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt
     2581 ggccagtgga tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca
     2641 aaagaagaca tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat
     2701 atctcttctc taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg
     2761 tatccttggg cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa
     2821 acagcgacat ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa
     2881 ccatttgata gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc
     2941 gttcaactat aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg
     3001 aaatttttgt ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa
     3061 ctaagacaga agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta
     3121 ttgacagaga aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat
     3181 atattcgtga ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg
     3241 gacaactagc agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg
     3301 tggaagcaag tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag
     3361 cacctagtgc agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt
     3421 tattgaaagt caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga
     3481 tcgacaaccg cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg
     3541 aagcattcgc tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt
     3601 tttctggcaa aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt
     3661 ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg
     3721 agacagaaga atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag
     3781 aaaaaggtcg gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg
     3841 caaaaatggc ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg
     3901 taaaagaagt acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa
     3961 acgggatcga actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg
     4021 tgaataatcc ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc
     4081 gtttataaaa atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg
     4141 tgtcaagaga aggatcggaa aagacgaata taacaaaaaa agagcgtg
//
LOCUS       DAZKAY010000152         4116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000152 DAZKAY010000000
VERSION     DAZKAY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.159"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..316
                     /locus_tag="ACG8WP_002717"
     CDS             155..316
                     /locus_tag="ACG8WP_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9028966.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="ACG8WP_002718"
     CDS             335..1612
                     /locus_tag="ACG8WP_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028967.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="ACG8WP_002719"
     CDS             1622..2278
                     /locus_tag="ACG8WP_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9028968.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="ACG8WP_002720"
     CDS             2278..3654
                     /locus_tag="ACG8WP_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9028969.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="ACG8WP_002721"
     CDS             3784..>4116
                     /locus_tag="ACG8WP_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9028970.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAZKAY010000153         4027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000153 DAZKAY010000000
VERSION     DAZKAY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.160"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..382)
                     /locus_tag="ACG8WP_002722"
     CDS             complement(233..382)
                     /locus_tag="ACG8WP_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028971.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            844..1056
                     /locus_tag="ACG8WP_002723"
     CDS             844..1056
                     /locus_tag="ACG8WP_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028972.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1334..3133)
                     /locus_tag="ACG8WP_002724"
     CDS             complement(1334..3133)
                     /locus_tag="ACG8WP_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9028973.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3257..3853)
                     /locus_tag="ACG8WP_002725"
     CDS             complement(3257..3853)
                     /locus_tag="ACG8WP_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9028974.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1444 a          711 c          689 g         1183 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa tataaatcat
      601 atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg aacaacatag
      661 gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat agttatctaa
      721 aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt ttcaaataca
      781 aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa aagagggaat
      841 gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg agtatctcat
      901 gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta tgttgaaagt
      961 tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct cgtaaaattc
     1021 tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag aattcgcaag
     1081 aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca aaaagaaaaa
     1141 gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct attttagcta
     1201 ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc gtcaaacaaa
     1261 aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga cttttttcat
     1321 taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct gaataagtga
     1381 tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg gttatgatac
     1441 catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt gtccatacat
     1501 atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc gtactatatt
     1561 ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa ggtaagatgt
     1621 agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg tacgtcaaac
     1681 tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca aatcgttcga
     1741 caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt tggtttagct
     1801 tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact ccgttttcag
     1861 taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca tttacccctg
     1921 ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt gtttcctgaa
     1981 catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt gcttctaaag
     2041 tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact ggttttgtct
     2101 gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg aaaaaaactg
     2161 caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg gcttgtggga
     2221 taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca acaatgatat
     2281 aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt aaaagcgtca
     2341 tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc atactttgtg
     2401 ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg ccaatcacaa
     2461 taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt tcccgaatgg
     2521 ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc agtcgaatcc
     2581 caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc caacgattag
     2641 caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga tcagaattaa
     2701 aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac aaaaaagtaa
     2761 tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc aaagagattg
     2821 gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg aaataaacta
     2881 acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga tgttgagaaa
     2941 aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt aaaataaaac
     3001 gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc atcagcaaga
     3061 catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct aagctacgtt
     3121 tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa tgaaaaagga
     3181 aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat caatgactaa
     3241 ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca taaatcaagg
     3301 ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt gtatatcgtt
     3361 tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga gcaactggaa
     3421 gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc cagccaatat
     3481 atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca taacgacgcc
     3541 ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag ataaatccag
     3601 cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga atgattggaa
     3661 taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg atggaatcac
     3721 ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata aaataaatcg
     3781 tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt tttcgagagg
     3841 cagcaatatt cattattcat caactcctta gaggttcttt gttaactata ctttactaga
     3901 aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt gtaagttcat
     3961 cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt taataacttg
     4021 ttttttt
//
LOCUS       DAZKAY010000154         3946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000154 DAZKAY010000000
VERSION     DAZKAY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.161"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACG8WP_002726"
     CDS             complement(93..635)
                     /locus_tag="ACG8WP_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9028975.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="ACG8WP_002727"
     CDS             770..1159
                     /locus_tag="ACG8WP_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028976.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="ACG8WP_002728"
     CDS             1163..1927
                     /locus_tag="ACG8WP_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9028977.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="ACG8WP_002729"
     CDS             2056..3003
                     /locus_tag="ACG8WP_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9028978.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="ACG8WP_002730"
     CDS             3133..3873
                     /locus_tag="ACG8WP_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028979.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1308 a          672 c          802 g         1164 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatc
//
LOCUS       DAZKAY010000155         3945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000155 DAZKAY010000000
VERSION     DAZKAY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.162"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(595..1152)
                     /locus_tag="ACG8WP_002731"
     CDS             complement(595..1152)
                     /locus_tag="ACG8WP_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028980.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1331..1942)
                     /locus_tag="ACG8WP_002732"
     CDS             complement(1331..1942)
                     /locus_tag="ACG8WP_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028981.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2055..2312)
                     /locus_tag="ACG8WP_002733"
     CDS             complement(2055..2312)
                     /locus_tag="ACG8WP_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028982.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2909..3187)
                     /locus_tag="ACG8WP_002734"
     CDS             complement(2909..3187)
                     /locus_tag="ACG8WP_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028983.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3221..3472)
                     /locus_tag="ACG8WP_002735"
     CDS             complement(3221..3472)
                     /locus_tag="ACG8WP_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028984.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1198 a          809 c          523 g         1415 t
ORIGIN      
        1 aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc
       61 cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat aatttattca
      121 tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaatttc
      181 ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca ctttttaagt
      241 tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta
      301 gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc
      361 tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac
      421 tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg ctctcttttt
      481 tattcagatt catttttgct gatcacagcc tagtatttca atggttttga acactaaacc
      541 aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt tcgattattc
      601 aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc caaacctatc
      661 gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt tattgcgtgg
      721 atattctatt gttacgccat taaatggata ccaattagga ttaggctctc tttttttctg
      781 tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc gaccatcctc
      841 taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta gacgccacgt
      901 gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg caacaattgt
      961 atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat caaactcccc
     1021 tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta aaaccgcatt
     1081 cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt ttctatttga
     1141 taaatttctc attattcgca catcacctct acattctcac atattagttc gtaatactcc
     1201 tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct actactcgta
     1261 taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt ctatttatcg
     1321 taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc cagttgatta
     1381 aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt acagttctta
     1441 tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc tcgagatttc
     1501 taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata taatattgcg
     1561 cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct tctctaccac
     1621 tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg aactcacacc
     1681 cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct agttcgtatt
     1741 caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta ggtgagatta
     1801 atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct atgatcacaa
     1861 ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg tattccattt
     1921 tattccccct attactattc atatccgcct atctatatcg atcttccaat gcagagataa
     1981 ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt ctccactaag
     2041 gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac tcctccaaaa
     2101 acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt atatgtgttt
     2161 gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat cgcattcgta
     2221 tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat cattgtgatt
     2281 tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta tagaaatcgc
     2341 ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg tctccatttt
     2401 tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca caacacgtta
     2461 ccttttaaat cattatactg tatctcatac attattatca ataaactatt ataaaaatgc
     2521 aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa tcataccctg
     2581 tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg caagacagac
     2641 actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt acccactttt
     2701 gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac cccaagaaat
     2761 taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca acatttaaat
     2821 tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata tcaagatgtc
     2881 aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta ttaagactaa
     2941 tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat gatcctgcaa
     3001 gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc tttttcccat
     3061 taggatcttt tattttatga attttctgca ggtaatctct cctaatggca gctattttta
     3121 cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt atgttgtgtt
     3181 tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa agttgaatga
     3241 gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca tatcctcccg
     3301 caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa gagaaaagag
     3361 tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag ataacaactc
     3421 caaacattga atttttatac aatgaccatt tatttatagg cttttctttc attttacttc
     3481 cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc tctttgaaca
     3541 tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt tataggcgag
     3601 ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact gaaattaaag
     3661 cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac atgtctttac
     3721 ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg taatactttt
     3781 tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata atatctaatc
     3841 taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa ataaatagaa
     3901 actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DAZKAY010000156         3909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000156 DAZKAY010000000
VERSION     DAZKAY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.163"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACG8WP_002736"
     CDS             complement(<1..242)
                     /locus_tag="ACG8WP_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028985.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTA"
     gene            375..749
                     /locus_tag="ACG8WP_002737"
     CDS             375..749
                     /locus_tag="ACG8WP_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HFP9028986.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            803..2689
                     /locus_tag="ACG8WP_002738"
     CDS             803..2689
                     /locus_tag="ACG8WP_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028987.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQVKSRLLCKMLYNVFTIS
                     TYQN"
     gene            complement(2673..3845)
                     /locus_tag="ACG8WP_002739"
     CDS             complement(2673..3845)
                     /locus_tag="ACG8WP_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFP9028988.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1345 a          600 c          553 g         1411 t
ORIGIN      
        1 atagctgtca ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata
       61 gaatttatgt tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt
      121 gttttgttat tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca
      181 tattcttgag tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc
      241 attttttctc ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg
      301 ccaaaaaagt tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat
      361 aggagaaata agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa
      421 ctagtggagg aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata
      481 ccgccattta cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga
      541 gatatgctgc tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa
      601 attactacca tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag
      661 tacgatcagt tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg
      721 gaaatactgg ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta
      781 catttgaaag gaaattcaaa atatgttaaa aaaaataaac gctgttttat cactttttgt
      841 ctgcattaca atactatttt tttctactaa tgttataaat ggtataaaga cttctgaagc
      901 gctatataaa aataccgctg aaattcatat gcaatataaa gatgatatgg actttaacag
      961 ttatataaac gctttgtatg aaatatctac acaagaaaat attaatattt ctcaatatag
     1021 ttttactgct gttaaccaac taactattct tgctacaaac cctaacgcta acaaacactg
     1081 gaaattttct aaaaaaaata tattagcaag aaacgattct aaagtccatt atactaataa
     1141 caaaaattct aagtaccaat taaaacttcc aaataatttt ttaaatataa atatttatcc
     1201 tttttctcat gtggaaaata ttggattaag tgaaatatta tatgtacaag gaaactcaaa
     1261 caaattaatt cctgtactga aaaaatatgg aacacctact atttccaata tcaatcaatc
     1321 agatactttt caaattaata ctaatattgt tcttgttatc tgctatctta gtatatttat
     1381 tgtaattact acaattatat ttgctttttc aaaaatgaaa gaaatcactc taaaaaaaat
     1441 gttaggctat aactcttttg atactataaa agattctatt aaagagttaa acctcttatt
     1501 ttgttctttg attataactt tcattttatc tatttttttg agcatatatt acagtaacga
     1561 aaattttttc ctatcatttc ttctatgtag tatttttcat tttataatta gttttttatt
     1621 tatcatagtt actctaagta tctatcattt atctagtata aaaaattatc taaaaaattc
     1681 acgaccactt aagttaaatc tttgtatatt aaatatctgt ctattcttaa gcatgatcct
     1741 tctgttaata gcttctacaa aagtaataaa tacccataat gaagccgaaa ataattctct
     1801 taaatattgg gaaagaacta ctaatttata caaaacaaat attactaatc aattaaatag
     1861 aaacaacacc gtagaagaaa ataactactt gaaaaaagct agtaaatttg tttataaaat
     1921 acagaagaat tataaaactt ttattatcgc gccatacaat tacgcaacta tccaagagaa
     1981 taataaagaa ttttttattg gacaaaaagt ttatccaaat tttgaagact atgttagtca
     2041 acctgctggt gcaggaatat gtgttgattt aaactatcta aaatattaca atcctatttc
     2101 ttttgaagaa tctactgact taaatttaat aaactctgat ccactaacta cttacattct
     2161 agtacctaaa aaatataaag aatatgcaaa aatgattgaa aaaaattatt tagaagacat
     2221 acagtttaga cttgaggact ctcctcctca agctccttat aaaaatccta atttgaaaaa
     2281 tttaaaaata aaactaatat ttgttaataa taaccaaaag tattttagtt ttaatacctt
     2341 atatggaaag gcaaaagata actatacaat aactgatccc atagttagag ttattaatcc
     2401 agaaataaca gaatcattat tttggggaaa tatattgact agtgatggtg gattattttt
     2461 tgatcagaaa catacttcaa accaaaactt ttttaatcaa attaatccgt atataaaaga
     2521 atttaaccta gaaaatatca taaatttttc ttattctgtt tttcaagaaa ctggtgaatt
     2581 attatctcaa aaaaaagtaa ataaattaaa tgcatatcta caagtcaagt ccagactcct
     2641 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     2701 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     2761 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     2821 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     2881 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttgatcg
     2941 atataatcat gaatcaatcg atttttagcc tcacgcgcta agttaatatc tgtgaactta
     3001 aaaattcctt taacagcttc tctgaaagat tttgaatttt ttttaggaat ggtggtaaag
     3061 atatttctta ggaagtgaac ttggcatctt tgccaactta cgttggtgaa ggattttcta
     3121 atggcagaga ctaatccttt gtgcgcatca gaaataacga gttccgtacc ttgtaaaccg
     3181 cgttctttta ggtattcaaa aaatgttgtc caggtctctt cgctttcgcc actttgaatc
     3241 atgaagccga taatttcacg gtcgccatct ttggttattc caatcgctat atgacagctt
     3301 tttgagagta ctcgattttc ttctcgtact tttatataga gtacatcggt cattaagtaa
     3361 ggataatttt tttctgataa taaacgattc tgccactcgt taaccatagg ttctagctgt
     3421 tctgttaagc tagaaacgaa ggacttagag acggatttac cacaaagttc ttccacaatt
     3481 tttgatactt tacgagttga aacgcctgat acatacattt ccaacattga agccatgagg
     3541 gctttttcgt ttcgttgata acgttcaaac actgtgggtg aaaaatggcc atcacgtgtt
     3601 ctgggtactt ttaattctag cgtgcctaca cgtgtcgtaa agctgcgctc ataatagcca
     3661 tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat atattctgtt
     3721 cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga aacgtcatcc
     3781 tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa atgtacttgg
     3841 gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac cgtaaaagga
     3901 ctgttatat
//
LOCUS       DAZKAY010000157         3896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000157 DAZKAY010000000
VERSION     DAZKAY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.164"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG8WP_002740"
     CDS             289..1104
                     /locus_tag="ACG8WP_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9028989.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG8WP_002741"
     CDS             1230..1487
                     /locus_tag="ACG8WP_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028990.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG8WP_002742"
     CDS             complement(1739..2311)
                     /locus_tag="ACG8WP_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9028991.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8WP_002743"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8WP_002743"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9028992.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3333..>3896)
                     /locus_tag="ACG8WP_002744"
     CDS             complement(3333..>3896)
                     /locus_tag="ACG8WP_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9028993.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1294 a          715 c          582 g         1305 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa
     3121 catggcatac tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat
     3181 ttcctttatt tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat
     3241 ctctatacta caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa
     3301 tctagaacgt aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt
     3361 tttgcagcct ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca
     3421 accgctagta aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca
     3481 ctataagcaa gagccattcc taataaatag ccaattaacc ctcctgaaag agttaaaatc
     3541 aaaccttcta aaaggaactg cagcataatc gattttcgag tagctcctag ggctcttctt
     3601 ataccgatct cttttgttcg ttcagataca gatatataca tcatgttcat tacacctacc
     3661 cctgcgataa ataaagatat acctgctaca gctgttataa aataagtgat cgtactgagg
     3721 atttgaccaa ttcctttcgt taacatagcc gtatctaaca cttcatattc ccccaaatgt
     3781 ttcaaactac ctttttcttt caattggttt atcactttag aggtcacctt gtcaggttca
     3841 actccttcct ctaaagttaa cgttagagac gatgtatcct tttcagactt aaaata
//
LOCUS       DAZKAY010000158         3856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000158 DAZKAY010000000
VERSION     DAZKAY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.165"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..301)
                     /locus_tag="ACG8WP_002745"
     CDS             complement(50..301)
                     /locus_tag="ACG8WP_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFP9028994.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(315..506)
                     /locus_tag="ACG8WP_002746"
     CDS             complement(315..506)
                     /locus_tag="ACG8WP_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9028995.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            799..1119
                     /locus_tag="ACG8WP_002747"
     CDS             799..1119
                     /locus_tag="ACG8WP_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9028996.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1137..1559
                     /locus_tag="ACG8WP_002748"
     CDS             1137..1559
                     /locus_tag="ACG8WP_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9028997.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1642..2277
                     /locus_tag="ACG8WP_002749"
     CDS             1642..2277
                     /locus_tag="ACG8WP_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9028998.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2395..3606
                     /locus_tag="ACG8WP_002750"
     CDS             2395..3606
                     /locus_tag="ACG8WP_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9028999.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1417 a          618 c          682 g         1139 t
ORIGIN      
        1 ctatttcccc aaagccctaa agcgtagatg attagcggtg cgctgaatac tagtgctctg
       61 ttgaatttgc ccatgttttt cctcctttta gatactgata caaagcgata ctactaaacc
      121 tccactctct gccaactttc gctgctggaa tctttccaga ttcggcatct ttagttagcg
      181 tgcgtgtcgt ggttttcaaa tattccgcag cttgctttgt atcccacact tcatttgcaa
      241 tctcagattc tgccaaagaa gctttgaggg ctgagaggtt aactaaagct agttgttcca
      301 ttgattaatt cctcctatcg aattctgtaa tcacggatga tttctaagat agttttatta
      361 gcttttggtc cactccaatg gccatcaata atttgctgca ttcgaacacg tgtgtatcca
      421 tatgcagtag ccaaatcttc catagttact ccgttttctc gcataaactt cttgattgca
      481 gcacggccgt tatctagatt tgacatctat tcactcccct tacatataga tttgtaagtc
      541 aaaatgatag aaaaaacgta taaactattg actaatagta tacaatcgta tactatataa
      601 acatagttaa ataagcctac aacaaaccct ttattatgca atcggtcgcc aaacttaatg
      661 ctataaggtg tatttttagt ttgctttttt tctatcaaat taacttacaa acaaataata
      721 atacaaactt atacttatgt caacagtata atttacattt tgtgtacttt tatttgttta
      781 ggattggaga acattattat gacactgttt gaaaggataa aatcattagc tagccaaaga
      841 gataaaagca tgaaagaagt cgctttagaa ttaggattta gcgaaaatct tttctatcga
      901 tggaaaacaa cagaacccaa agcaagagat ttgcaaaagg tagctgacta tttcaatgtc
      961 tctgtagact atcttctagg tagagaagaa aaagaaacgc ctaaacatgt ggatttatca
     1021 gaagatgata ctgtattttc ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa
     1081 gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg ggttgtatgt
     1141 atttaaagtt gaaagaaatg ctgagtgagt ataatttaaa gttaatctat atggaaatgg
     1201 aagaaccagg tttttattat ccaaaaccaa gaatagtatt tttgaatgaa aaactacacg
     1261 aagacagttc tgaagctttt catttagccc atgagctcgg tcatttcatt gcttcacatt
     1321 ttgaatattc agtactgtac gataactcta caacttttca ttcaaaattc gaagctgaag
     1381 ctgatagaat cgcaattatg attttactaa atatctttat tgagaacgaa ttgactgatg
     1441 aatctcagtt caaattagaa aatttcatgg aattctactc tatcaataat aagttaagaa
     1501 cagaatgttt taatgtttgc cagtcatatt tcaagaaaaa atactcttat gcacagtaaa
     1561 aaaagcccgt gctgcaacac ggactcatac ctcatttctg agatcacaaa tatattataa
     1621 caagaagtga gggatattta aatggcaaaa aaagttatgg gtcaagatgg gaaaatgtat
     1681 aaggttaaaa aaccgtttta taaacgggta tggttttggg tattaatagt acttttgttt
     1741 tttggtattg gtggtgctct aagtggcggt gaaaacaaag attcagctac tgcaacatca
     1801 acaagtacta ataaagaaac tacaaaagaa actacaacaa aagaagaaaa gacttataaa
     1861 atcggtgatg acgttgctgt agggaaaatg gaatacaaag taaactccgt tgaagtagta
     1921 aaacaagttg gcccatctgt acttcctact aacgcaaaag acactttctt agtagttgac
     1981 ttatctgtaa aaaatgcagg tgataaggct gtgactgttg atagctcatt cttcaaatta
     2041 aaagcagatg gaaaaacttt cgaagctgat tcggctgcat ctatgtcggc aaatcaagat
     2101 gaaaatggta atatcactaa ctctttcttt atggaaagct taaatcctga tatgcaacaa
     2161 actggtaaaa tagtttttga tatctctgaa gcccaagcaa atgcacaaaa taatgtattg
     2221 caagcacaaa caggttactt tggaacagag acagtttcaa ttgctcttca taactaatta
     2281 gtataaaaaa cacgccccac cgaccaaagc gagcgtgttc taagaaaaaa caaacctaca
     2341 caataggctt ctttatagta cctattgtat cagagaaaga gagctgattc aattatgtca
     2401 gaagtaacag gatacttaga acaagtagac aatgaaacat atagactaag agctgtatta
     2461 gggtacaaac ctgatggctc ggcaaaaaga aaaagcaaaa ctataaaagc gaatagtaaa
     2521 agagcggcat ataaagaatt gaacatctgg ttagagcaat tcgaaggaat gaccgatgat
     2581 tcgttggatt tattcaatat aacttttggt gaattttata gaaaaatatg gctttcagag
     2641 gccgaaaaaa atttagagcc aaagtcttat cataactata agcgaatgat cgagaataga
     2701 tttcttgata aatttgattt tatccctcta atcgatataa aaccatacat gataaagaaa
     2761 attgttgtta atgctcaaag aatcaataca aaagatcctg gaagaaactc tgataaacct
     2821 ttatcaagaa atacaaagct tcgtatgtta tacgccgtta acaacttatt tttgatggct
     2881 aaaaatgagt acggagcaat taaagaaaat cctgtggaaa atgtaaaaat accaaaagaa
     2941 aaaggcgtaa aaaagaatat cgaagaacct tattcggaag aagaaattca tgcaatgcta
     3001 aaagcagcat ttgaggaaag catagaaatt aaaactttaa ttgttctagc atttattaca
     3061 ggagcaagac aaggagaaat tgcagcttta gaagaaaaag atattgactt tgacaaacaa
     3121 gaaataagat ttcatcagcg gatttcagaa gtggatggaa aatcagatat tcggttgctg
     3181 ccaggtttga aaaatgatga tgatgaaaaa atagtaaccg gtcctgctta tctatttgat
     3241 atgttagatg aattgatcaa agagaataaa aaaattcgtt ggaaattaaa tataaagaaa
     3301 ttaagacact attttatatt tgatacaaaa caagacggaa ctttaccacg tggaagttat
     3361 ctgtataaga agttcaaacg attcaccaaa cgtcacaatt tgcgtcacat tcgttttcac
     3421 gacattagac atacatcagc tacctatttg cttagcgatc ctaatatgac tccaaaagag
     3481 cttcaaaaac gattaggaca ccgagatttt aatactacaa tgaatgttta tggccatgtc
     3541 ttacgcaaag aaaaagatac agcaactaca gcgtttgaaa aactattaaa aaaagatata
     3601 aaataacact aaaaaattat attttcgggt ataaactcgg gtaggatata taaaaacttg
     3661 ggataatttg cggtatataa gcatttaaaa atgctgtaaa ttcgcttatc ttcatttaaa
     3721 ataccattaa ataacgaact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
     3781 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
     3841 cgctgttctt ttttta
//
LOCUS       DAZKAY010000159         3827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000159 DAZKAY010000000
VERSION     DAZKAY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.166"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1435
                     /locus_tag="ACG8WP_002751"
     CDS             86..1435
                     /locus_tag="ACG8WP_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9029000.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ACG8WP_002752"
     CDS             complement(1563..2153)
                     /locus_tag="ACG8WP_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9029001.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG8WP_002753"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG8WP_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9029002.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1086 a          706 c          752 g         1283 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgt
//
LOCUS       DAZKAY010000160         3774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000160 DAZKAY010000000
VERSION     DAZKAY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.167"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACG8WP_002754"
     CDS             <1..271
                     /locus_tag="ACG8WP_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029003.1"
                     /translation="TFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPL
                     DRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(397..1074)
                     /gene="nth"
                     /locus_tag="ACG8WP_002755"
     CDS             complement(397..1074)
                     /gene="nth"
                     /locus_tag="ACG8WP_002755"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9029004.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1081..1779)
                     /locus_tag="ACG8WP_002756"
     CDS             complement(1081..1779)
                     /locus_tag="ACG8WP_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9029005.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2047..3231
                     /locus_tag="ACG8WP_002757"
     CDS             2047..3231
                     /locus_tag="ACG8WP_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9029006.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3237..3680)
                     /locus_tag="ACG8WP_002758"
     CDS             complement(3237..3680)
                     /locus_tag="ACG8WP_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9029007.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          974 a          749 c          625 g         1426 t
ORIGIN      
        1 cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc
       61 caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac ttatcatttt
      121 tattacacct ttagatagat acgtcactta tttatataat cctttggaat actggactta
      181 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
      241 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      301 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      361 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      421 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      481 tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg
      541 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      601 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      661 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      721 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      781 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      841 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
      901 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
      961 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
     1021 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
     1081 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
     1141 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1201 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1261 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1321 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1381 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1441 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1501 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1561 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1621 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1681 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1741 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1801 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1861 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     1921 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     1981 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     2041 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     2101 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     2161 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2221 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2281 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2341 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2401 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2461 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2521 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2581 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2641 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2701 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2761 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2821 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     2881 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     2941 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     3001 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     3061 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     3121 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3181 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3241 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3301 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3361 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3421 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3481 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3541 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3601 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3661 agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt
     3721 ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa ccaa
//
LOCUS       DAZKAY010000161         3393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000161 DAZKAY010000000
VERSION     DAZKAY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.168"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8WP_002759"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8WP_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9029008.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8WP_002760"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8WP_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          953 a          747 c          538 g         1155 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaa
//
LOCUS       DAZKAY010000162         3391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000162 DAZKAY010000000
VERSION     DAZKAY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.169"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1831
                     /locus_tag="ACG8WP_002761"
     CDS             131..1831
                     /locus_tag="ACG8WP_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9029009.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1801..2019
                     /locus_tag="ACG8WP_002762"
     CDS             1801..2019
                     /locus_tag="ACG8WP_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9029010.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2039..2929
                     /locus_tag="ACG8WP_002763"
     CDS             2039..2929
                     /locus_tag="ACG8WP_002763"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9029011.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2947..3231
                     /locus_tag="ACG8WP_002764"
     CDS             2947..3231
                     /locus_tag="ACG8WP_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029012.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1197 a          551 c          721 g          922 t
ORIGIN      
        1 tgtgattttt gtattttttt atttaaagga ggttttatga attgttttct tatggaatct
       61 acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact tgaagaaaaa
      121 ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc
      181 tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa gtagagacgc
      241 tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt
      301 ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt tgattgaagg
      361 tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc gtttttttat
      421 taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt tgcgtatttt
      481 tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct atcatacagc
      541 acttgtttta ggtggaggag gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct
      601 gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg cgttgaacgg
      661 cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac
      721 aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta
      781 tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt cttctgctcc
      841 attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata atcccaaaat
      901 acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga ctgatatcca
      961 acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat tttatcccgc
     1021 tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat
     1081 acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag atgtgcaagg
     1141 gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga aatgtcaaac
     1201 attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat taaatttaca
     1261 attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact ggtatacatt
     1321 tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact taacaaaaga
     1381 aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt
     1441 atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac ttttagcaaa
     1501 aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc aaatgatctg
     1561 ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc aattaagtac
     1621 tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac
     1681 tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc atgaattgct
     1741 taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg aggaacaacc
     1801 atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac
     1861 aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc aaaattcgat
     1921 cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa
     1981 gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac cctgtcgaat
     2041 gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc agttagatat
     2101 tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc ctaaaatcct
     2161 gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc gcggtcgaat
     2221 cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg tcatcaactg
     2281 ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac gatacggtag
     2341 tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gcgctggacg aaaacttcgt
     2401 aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag ctctagatac
     2461 attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc aagctttagc
     2521 tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt
     2581 tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg aggaatttct
     2641 tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt tggaacgcgg
     2701 agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag atgaacttat
     2761 cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa tgaatcggga
     2821 aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc
     2881 taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac gaaagaggta
     2941 gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa
     3001 cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg tgcagactat
     3061 aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat gaatcggaaa
     3121 tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc acgttctcga
     3181 aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta gcaaggatgc
     3241 tgaacaaaga aatacaataa ataatcagaa ttggatttct tcgcttagct tctttgctct
     3301 aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa
     3361 gtgatgttca aaaaccattt tctcggtatt a
//
LOCUS       DAZKAY010000163         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000163 DAZKAY010000000
VERSION     DAZKAY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.170"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8WP_002765"
     CDS             301..585
                     /locus_tag="ACG8WP_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029013.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8WP_002766"
     CDS             588..1004
                     /locus_tag="ACG8WP_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029014.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8WP_002767"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8WP_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9029015.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8WP_002768"
     CDS             2544..2630
                     /locus_tag="ACG8WP_002768"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9029016.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8WP_002769"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8WP_002769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029017.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZKAY010000164         3040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000164 DAZKAY010000000
VERSION     DAZKAY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.171"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8WP_002770"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8WP_002770"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9029018.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8WP_002771"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8WP_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9029019.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8WP_002772"
     CDS             2069..2821
                     /locus_tag="ACG8WP_002772"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9029020.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1046 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat
//
LOCUS       DAZKAY010000165         2977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000165 DAZKAY010000000
VERSION     DAZKAY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.172"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACG8WP_002773"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACG8WP_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACG8WP_002774"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACG8WP_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACG8WP_002775"
     CDS             1941..2351
                     /locus_tag="ACG8WP_002775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029021.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..>2977
                     /locus_tag="ACG8WP_002776"
     CDS             2432..>2977
                     /locus_tag="ACG8WP_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029022.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCH"
BASE COUNT          985 a          448 c          547 g          997 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcat
//
LOCUS       DAZKAY010000166         2971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000166 DAZKAY010000000
VERSION     DAZKAY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.173"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..296
                     /locus_tag="ACG8WP_002777"
     CDS             <1..296
                     /locus_tag="ACG8WP_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029023.1"
                     /translation="DCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(382..1476)
                     /locus_tag="ACG8WP_002778"
     CDS             complement(382..1476)
                     /locus_tag="ACG8WP_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9029024.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1665..2426
                     /locus_tag="ACG8WP_002779"
     CDS             1665..2426
                     /locus_tag="ACG8WP_002779"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9029025.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2541..>2971)
                     /gene="fabZ"
                     /locus_tag="ACG8WP_002780"
     CDS             complement(2541..>2971)
                     /gene="fabZ"
                     /locus_tag="ACG8WP_002780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9029026.1"
                     /translation="LSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTINEDFF
                     QGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVPGDVL
                     KCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          953 a          588 c          508 g          922 t
ORIGIN      
        1 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
       61 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
      121 ttctgcgtcg taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta
      181 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
      241 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
      301 tcataaccta taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt
      361 atttctgcaa taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg
      421 tttcaaaaag tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg
      481 tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc
      541 ttcattgtca atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc
      601 tttatctttg tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc
      661 tcgataacta tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc
      721 cgtatctact ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc
      781 ttcttcactt gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa
      841 atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc
      901 ttgcgtaggc caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa
      961 attatatttt ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta
     1021 tcgatgagga taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat
     1081 gatgttgtca acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg
     1141 attgcgcgtc atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta
     1201 ggcacccatc ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa
     1261 cgctggattg atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc
     1321 tttaaattct gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac
     1381 ccgtttatac ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga
     1441 ccgttctgga taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt
     1501 tcgtttttgt atccacttac attatagcaa acacaaagta aatggtctac accttttctt
     1561 tttcttttaa aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg
     1621 ttatcattaa agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag
     1681 ttattttaag tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca
     1741 acacgacttc ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca
     1801 cgactgccag tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg
     1861 ccgaaaatga agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat
     1921 ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact
     1981 acaacggact gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta
     2041 aaggcgatgg cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca
     2101 accaccttta taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta
     2161 acggcagcca gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag
     2221 acgactatcc tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg
     2281 gccattatac tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa
     2341 atatcgaaac agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata
     2401 tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac
     2461 gtatgctagg attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt
     2521 tgctatatca ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca
     2581 cagacttttt tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt
     2641 ttggtgattt caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg
     2701 gccttattga tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata
     2761 agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg
     2821 tcaggaaagt gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc
     2881 ttttttcctg gttctaaagt atctacataa tcaatataac aaataggata acgattagga
     2941 atcaattcca ttacttcagt agcagataat a
//
LOCUS       DAZKAY010000167         2954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000167 DAZKAY010000000
VERSION     DAZKAY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.174"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACG8WP_002781"
     CDS             89..406
                     /locus_tag="ACG8WP_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9029027.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACG8WP_002782"
     CDS             complement(497..862)
                     /locus_tag="ACG8WP_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029028.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACG8WP_002783"
     CDS             1070..1552
                     /locus_tag="ACG8WP_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029029.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="ACG8WP_002784"
     CDS             1749..2639
                     /locus_tag="ACG8WP_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9029030.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1009 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tgta
//
LOCUS       DAZKAY010000168         2490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000168 DAZKAY010000000
VERSION     DAZKAY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.176"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG8WP_002785"
     CDS             complement(365..1657)
                     /locus_tag="ACG8WP_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9029031.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG8WP_002786"
     CDS             complement(1931..2191)
                     /locus_tag="ACG8WP_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029032.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          381 g          800 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaaggcgct tctgtagaat
     2401 aaagttaacg acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag
     2461 aaattgtgca aactctagtc actaaaggcg
//
LOCUS       DAZKAY010000169         2456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000169 DAZKAY010000000
VERSION     DAZKAY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.177"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..2306
                     /locus_tag="ACG8WP_002787"
     CDS             153..2306
                     /locus_tag="ACG8WP_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9029033.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          839 a          428 c          543 g          646 t
ORIGIN      
        1 gatatgtaac tttaagtgat tgctgtgaaa caaaaagttg attttatctt gaaattaggt
       61 aaagaatttc ttgctccgcc cattttttct tttttattaa gaagtttttg ggtacaatga
      121 agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg aaaaaaatat
      181 taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag aacaaaacgt
      241 ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata tttacgttat
      301 ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa gagatggagg
      361 cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc ggaatgatct
      421 acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc acatatacga
      481 gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg aactcgctga
      541 tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt tggctggctg
      601 atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag caattaggga
      661 acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt aatttaaacg
      721 aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat gaattaaaca
      781 ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg gcagaacgcg
      841 cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat aatgcagcaa
      901 gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg
      961 aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga caaagttatt
     1021 tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg
     1081 ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt
     1141 taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg aaaaggaatt
     1201 atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat gcagtggcta
     1261 tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa caaggttcca
     1321 aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca gggtctgtag
     1381 tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga aatcaagttt
     1441 tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa
     1501 gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat acgtatatga
     1561 tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga ctacctagtt
     1621 tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag tttggtcttg
     1681 gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt tctgttgatt
     1741 atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt aactttaccg
     1801 atttggcatt tggacagttt gacacatata caccaatcca attagctcaa tatgcttcaa
     1861 cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc tatggcaatg
     1921 acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta gaaaatacgg
     1981 tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa gttgtccatg
     2041 gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac ctttccgcaa
     2101 aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca gtcaatagta
     2161 atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt gtcttgccga
     2221 acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata atggacgcgt
     2281 attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag
     2341 tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt gacttcatac
     2401 cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAZKAY010000170         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000170 DAZKAY010000000
VERSION     DAZKAY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.178"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8WP_002788"
     CDS             207..2069
                     /locus_tag="ACG8WP_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9029034.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8WP_002789"
     CDS             2069..>2398
                     /locus_tag="ACG8WP_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9029035.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKAY010000171         2307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000171 DAZKAY010000000
VERSION     DAZKAY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.179"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG8WP_002790"
     CDS             complement(136..426)
                     /locus_tag="ACG8WP_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029036.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG8WP_002791"
     CDS             complement(544..804)
                     /locus_tag="ACG8WP_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029037.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG8WP_002792"
     CDS             complement(941..1354)
                     /locus_tag="ACG8WP_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9029038.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG8WP_002793"
     CDS             complement(1810..2295)
                     /locus_tag="ACG8WP_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029039.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          843 a          376 c          366 g          722 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcc
//
LOCUS       DAZKAY010000172         2302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000172 DAZKAY010000000
VERSION     DAZKAY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.181"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1753
                     /locus_tag="ACG8WP_002794"
     CDS             140..1753
                     /locus_tag="ACG8WP_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9029040.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          721 a          407 c          447 g          727 t
ORIGIN      
        1 aaaaaatttt taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata
       61 aacaataatt ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg
      121 tagaaaagga gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc
      181 atttgccact cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg
      241 agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga
      301 ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt
      361 ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga
      421 cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc
      481 tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg
      541 tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt
      601 cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg
      661 cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat
      721 tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag
      781 agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc
      841 tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac
      901 cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct
      961 gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc
     1021 gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt
     1081 aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga
     1141 tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa
     1201 ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag
     1261 attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt
     1321 tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt
     1381 gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg
     1441 tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac
     1501 gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg
     1561 tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga
     1621 gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag
     1681 aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt
     1741 gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt
     1801 gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga
     1861 taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt
     1921 ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga
     1981 ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga
     2041 ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt
     2101 cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg
     2161 tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc
     2221 atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta
     2281 atttttttgt tcctatttat tt
//
LOCUS       DAZKAY010000173         2254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000173 DAZKAY010000000
VERSION     DAZKAY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.182"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            582..1541
                     /locus_tag="ACG8WP_002795"
     CDS             582..1541
                     /locus_tag="ACG8WP_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029041.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1528..2133)
                     /locus_tag="ACG8WP_002796"
     CDS             complement(1528..2133)
                     /locus_tag="ACG8WP_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029042.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          768 a          363 c          334 g          789 t
ORIGIN      
        1 taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat tagatatgaa
       61 aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag atgagagtca
      121 ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg atataggaag
      181 tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat ttttttgtta
      241 tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta tatcttctaa
      301 attagaagat atttgtatat caatatggaa tatattacct tttttgtatt tttttatgtt
      361 atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag ctggatttag
      421 tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt tttttatttt
      481 tttaccttcg ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact
      541 caaaatagtt ccgtcgaaag aattataagg agtgagtaca aatgacctat acccatctta
      601 caccaactga actcgtaatg atagaagctt atttcaatca atcacactct gtttcaaaag
      661 tagcccatct ggtacaacgt tctcgacaaa ccatttataa tgtctatcgt tttcttaaat
      721 ctggtggaag cgcaataagc tactatgaac aatataagaa aaacaaacgt aactgcggaa
      781 gacgtccgat tgtgttacca gatgggcaac aagaatacat tcaaaaaaag gtggctcaag
      841 ggtggactcc agatgtcctt attggtcgag cagagtttcc tattgcttgt tcggtacgta
      901 cactatatcg gatgtttaaa gataaaacct ttgaccctca cgatttacca atgaaaggga
      961 aaagaaaacc aaacggacat aaagaaaaaa gaggaaaaca agcttttcgc cgttcgattc
     1021 aggatagaac aactgattat atacagttca atcatgagtt tggtcaccta gaaggtgaca
     1081 ccattgtcgg tggaaaacat aaaagtgcgg tgatcacgtt agttgagcga ttatctaaag
     1141 tgatcatcac cttgaaaccc gctggtaggc aagctataga tattgagaat agactgaatc
     1201 aatggtttca atctgtaccg aaacacttgt ttaaatccat cacatttgat tgtggaaaag
     1261 agttctcgaa ttggaaaaag attagtaata ccaatgatat ttcgatttat ttcgctgatc
     1321 caggaacacc ttctcaacga ggactaaatg aacattcgaa tggcttatta cgtaaagatg
     1381 gcttatataa agctatggat tttaataagg tatccgaaac ctttatccaa tcggtggcat
     1441 caaaacgaaa tcatattcct agaaaatcat tacactatcg cacaccattg gaagtatttt
     1501 tgagttacgt aggtaaagat gatttgtcta gcttaatttg acaaacgaaa ctttgtaaaa
     1561 atattcgatt gaatattcta tttggcacga ctcacttggc agtatatgta tgtcaatatt
     1621 tttcatctgt ttcataggta tttcaaaatc taaactatta aaaacatata attccctgta
     1681 attatttaca tctaactcta ctaaatttat ctttggaaca atagatcctc cacccattcc
     1741 accataataa aaaaaatctt gttctctatc aaaatagcac atattaagct cagtttgttt
     1801 atcatcctca ataattttat aaatcaaacc tatataatca tgaatgctgg caaaaatttt
     1861 ttttaagtca agttctaaat cttcttcgaa aacacattta atttctccta ctgataattg
     1921 atactctata ttacatgact taattagttt tttagatccc ccctccagat aggcatatgt
     1981 ttcaataatt atgttctttt tattgcttgg caaatttccg ttatttattg cataaattaa
     2041 aaatgactgt ctatttagcc cataatctaa tacaacttct tcatactcat atttctttaa
     2101 atatttaata tgaaaatatg ctgaattaat caaaactcga gtgttactgt tgttatttat
     2161 tgttgttcta ttgtgtataa aattgtatag gttgtcatat tttcttggtt tagtgaacta
     2221 ctcaccactt agctaaacct aacggtttct tacg
//
LOCUS       DAZKAY010000174         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000174 DAZKAY010000000
VERSION     DAZKAY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.183"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8WP_002797"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8WP_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACG8WP_002798"
     CDS             complement(987..1952)
                     /locus_tag="ACG8WP_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9029043.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKAY010000175         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000175 DAZKAY010000000
VERSION     DAZKAY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.184"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8WP_002799"
     CDS             <1..159
                     /locus_tag="ACG8WP_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9029044.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8WP_002800"
     CDS             242..859
                     /locus_tag="ACG8WP_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029045.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG8WP_002801"
     CDS             1023..1355
                     /locus_tag="ACG8WP_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029046.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG8WP_002802"
     CDS             1679..1840
                     /locus_tag="ACG8WP_002802"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFP9029047.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG8WP_002803"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG8WP_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZKAY010000176         2074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000176 DAZKAY010000000
VERSION     DAZKAY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.185"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..800
                     /locus_tag="ACG8WP_002804"
     CDS             63..800
                     /locus_tag="ACG8WP_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9029048.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            828..1367
                     /locus_tag="ACG8WP_002805"
     CDS             828..1367
                     /locus_tag="ACG8WP_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9029049.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            1383..1553
                     /locus_tag="ACG8WP_002806"
     CDS             1383..1553
                     /locus_tag="ACG8WP_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029050.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(1865..>2074)
                     /locus_tag="ACG8WP_002807"
     CDS             complement(1865..>2074)
                     /locus_tag="ACG8WP_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9029051.1"
                     /translation="IFVTILGLFELIQMNFFSFTHGIFTWFALSGLIVIDILNYFYIY
                     YSYLKNEKFFYTMFFPLVILIILLS"
BASE COUNT          871 a          285 c          313 g          605 t
ORIGIN      
        1 gataaattgt gttttacatc ctcttatgtc tatgataaca taatttattg aggtgatttt
       61 tattgagtaa cgatatagtt cgatataata atggattaaa ttctgtccct ttaagaaaat
      121 ttacccctat agatatggat ttattttggg gaatatgttc taaaatgaaa agaaaaggta
      181 caaaagaaga aatttttaca tttaaacaaa taaaagaatt aattgattac aatataaatg
      241 cacgtggcga ccgttttgct aatgatttaa taaaaatgtc aaaaaaaatc attactttgt
      301 cttttattta tgaagacgaa aaaacatatg aacaattaaa tctgtttcaa aaatttaaaa
      361 tcgataaagt gaatgaaaca ttattaataa aggtttctga agaatttgaa tatatactta
      421 attcaatcgg aactaatttt actcgtttcg aattagaaaa tatgacaaac ttaaagagta
      481 gctacgttaa agagttatat cgccaactta tggctcataa agacagaaga tctaaaaaag
      541 gtcactggtt tgtaaaaata gaagatttta gagctctatt agcaatacca gaaagttatc
      601 gcatgagaga tattgataaa caaatttttg cccaagcgaa aaaagaattt ttaaacgtta
      661 attcaactca ccctgctatt tttaaaactt ttgaaatcaa aaaaattaaa gcctacaaag
      721 gaaataagat aggatctctg caaatttatt ttgaagaaaa tataccttca attaccttag
      781 ataattggtt agagaaataa tacctaacaa tttaggaggc aattatcatg caacccaaag
      841 aaacctttac aataaaggaa ttatctgaat tatttgaaat aagtaggcaa gcaatctcta
      901 aacatgttga taaattagac ccgtccttat tagctaaaaa tgagcgtggt tataaggttg
      961 tattacgttc tggagtattg caacttgcta gcaacttagg taaccaaagg ttactatata
     1021 ggttgcatga gtatgaacaa gaaaacgata aaaacgttta tatatcaagc aaaaacgtga
     1081 ttgtaaacga gttgctagat aagttgcgtg aagaaaatga atttttacga gaacaacttt
     1141 ccctcaaaga gcaaaataat tctaatatgc aaaaattact cgaccagcaa caacagctca
     1201 ccctacaggc aaatcaacag atcgaaaaac tgcaagaaca gttacaatta gcttatacgg
     1261 aagaaactcc agaagatgaa tcaactacta ttctttctga agaaacagaa tcgatagaaa
     1321 aacacacaaa aaagaagaag caaaaatggt ggcaactttg gaaataactg gagggaacat
     1381 tcatgacaag aaaaaatctt ctattcggtt ctattttact aggaattcta ggcttcagtc
     1441 cgatgttatt gtcagtaatc aataaaggga ataaacatgg tgaattgaaa ttgaatccaa
     1501 attattacaa cagtcctaac gaagaattgt ttgtaaaaga gacggataaa taataaaaaa
     1561 gactgctctt ccgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcacgc
     1621 gcttatgaag cgctaccaaa aatttaccat attttacaaa aaataacaag tcttaaaaaa
     1681 atgacagata aaaatgcaca aagaattaga tatagataac cgcgaaaaat caagctttag
     1741 aaagttgctt ttagctagac caattaaaaa tcacaaagtg agcgtctcta tccattggta
     1801 gaggggctac ttggcatagt gtacattatg tcaactatgc aattaaataa ctaactactt
     1861 catattagct taataaaata atcaaaataa ctagggggaa aaacattgta taaaaaaatt
     1921 tttcattttt taaataagaa tagtaaatat aaaaataatt taagatatct attactatta
     1981 atccagatag agcaaaccaa gtaaatattc catgtgtaaa gctaaaaaaa ttcatttgaa
     2041 tcaattcaaa taaacctaat atagtaacaa atat
//
LOCUS       DAZKAY010000177         2056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000177 DAZKAY010000000
VERSION     DAZKAY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.186"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACG8WP_002808"
     CDS             493..714
                     /locus_tag="ACG8WP_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029052.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACG8WP_002809"
     CDS             715..972
                     /locus_tag="ACG8WP_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029053.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACG8WP_002810"
     CDS             complement(1108..1710)
                     /locus_tag="ACG8WP_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9029054.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACG8WP_002811"
     CDS             complement(1777..1932)
                     /locus_tag="ACG8WP_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9029055.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAZKAY010000178         2004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000178 DAZKAY010000000
VERSION     DAZKAY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.187"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8WP_002812"
     CDS             <1..378
                     /locus_tag="ACG8WP_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9029056.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8WP_002813"
     CDS             426..1826
                     /locus_tag="ACG8WP_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9029057.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAZKAY010000179         1951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000179 DAZKAY010000000
VERSION     DAZKAY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.188"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8WP_002814"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8WP_002814"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9029058.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKL"
     gene            complement(1262..>1951)
                     /locus_tag="ACG8WP_002815"
     CDS             complement(1262..>1951)
                     /locus_tag="ACG8WP_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9029059.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          574 a          366 c          244 g          767 t
ORIGIN      
        1 taattttaca cgactatttt ttctcaaaaa aacaggtcta atgaaatctt ctatttctat
       61 ataatctata gactcactta ataatcgtgc tgtttttaaa ttcaaacctg ctgcgtaaaa
      121 attgtaatta aatatagaaa agccatttgt aaattgggaa ccagaaggtg taaaatcttg
      181 attgtcaata tgttgagttg caatataagc tgttcttaga gcattagaaa tagaaaaatc
      241 aacactactt ctattaatgg acgtaaaaaa agttgtagca caatccccaa tagcatatat
      301 atcttttata tttgtttgct ggtattcatt agttagatat gctccactaa catctctcaa
      361 gagttccttt ttgccaattg gactattaga tagaaatcct atagcttgta cgaccatatc
      421 catagagtat tgggtaccac ttgtagtgac tattttttca atcttttttc ttcctaaata
      481 ttttttagga ctttctgata gacaaagatg tattccattt tttgatagta tttcttcaac
      541 tttatctgaa aaactacggt cgtaataagt cgacaacact ctatctgcaa catcgaaaag
      601 atatacttct ttccctcttt ttcttatggc ttcagcaatt tcaactccaa tatatcctgc
      661 tccaataatt cctacttttt taatagtatt atcatctaaa gctttaaaaa tctctttagc
      721 atttggaaaa ttctttatcg aaaaaatatt agctaaatta ttccccggta catctaacat
      781 aatttgcttt gaacctgtag ctagtataag tttgtcatat tcttgggaaa atcttgtgcc
      841 atcttttttt acacaaataa tctttttatt ttgaatatca attttgtcaa ctgctgtctc
      901 caaaaacaac ttttttatct tagaatttag ttctcttcta ttttgataaa aaagatcttc
      961 taaattgcta acttctcttt ttatgaacaa cgatgtccca cattctaagt aactaatttc
     1021 tgatttttta tctatcagaa taacttcata atttgaatca cataagttta gaaatcgagc
     1081 tgcagctagc cccgaatggt ttgatccaat aattactatt tttttcactt aatcacattc
     1141 ttttcccttc tatgaaaagt atcttcattt ttaactaaca acctatctcc caaaataaaa
     1201 aacaaaaaga ggaagaaaaa gattcttcct cttttcagag aagatttaag aaattgattt
     1261 tctattcatt ctcttttttt cgatttttat accctaataa ccctaaagtt cctaacagga
     1321 ttaaaccacc actaatagta aagatattac tatcaatctc tcccgtctta ggataagctt
     1381 ttgaggactg cttttgagga ctgcttttgc ctctattttt gttttgcttg ctaccttgat
     1441 tgtcagtctt attatcttta ctaacggctt tttcactgtc tttttcgttt gaagaaacaa
     1501 ctacattagt aggttcacta gtattaccag attcatcagt tgcagttact atcacttttg
     1561 ttcctgcctc taatttagga actttaactg taaaattacc atttttatca gctgtcccat
     1621 ggtattgact cccgtttggt aatgtaactg tcacctctgc atttggttca gttttaccag
     1681 taacaatttg gtcaccctct gtaataggat caacttgagg tttattcggt gcttttttat
     1741 cttctacaga aatagtaacc tctacttctt ctttagatcc atctggatat gttactacta
     1801 caatcccttt tttgtttcca gctgtactaa catctacttt ctctttccaa gtataagtag
     1861 ttccttttgg tagatcttct ttgttcttaa ttccctcaga tgcgtctggc tctttattta
     1921 agtctgtagt cactttttgt ccttcaggat t
//
LOCUS       DAZKAY010000180         1904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000180 DAZKAY010000000
VERSION     DAZKAY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.189"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..685
                     /locus_tag="ACG8WP_002816"
     CDS             <1..685
                     /locus_tag="ACG8WP_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029060.1"
                     /translation="KSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKN
                     IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSI
                     FSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPS
                     MMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAEL
                     RKLVEYGII"
     gene            672..>1904
                     /locus_tag="ACG8WP_002817"
     CDS             672..>1904
                     /locus_tag="ACG8WP_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9029061.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKIT"
BASE COUNT          643 a          242 c          296 g          723 t
ORIGIN      
        1 gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat taaataaaaa
       61 tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat tgttacttta
      121 tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa
      181 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
      241 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      301 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      361 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      421 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      481 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      541 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      601 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      661 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      721 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      781 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      841 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      901 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
      961 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
     1021 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
     1081 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
     1141 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
     1201 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
     1261 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
     1321 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
     1381 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
     1441 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
     1501 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
     1561 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
     1621 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
     1681 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
     1741 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
     1801 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
     1861 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacg
//
LOCUS       DAZKAY010000181         1859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000181 DAZKAY010000000
VERSION     DAZKAY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.190"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8WP_002818"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACG8WP_002818"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9029062.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAZKAY010000182         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000182 DAZKAY010000000
VERSION     DAZKAY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.191"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8WP_002819"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8WP_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFP9029063.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8WP_002820"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8WP_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9029064.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZKAY010000183         1853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000183 DAZKAY010000000
VERSION     DAZKAY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.192"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..473)
                     /locus_tag="ACG8WP_002821"
     CDS             complement(75..473)
                     /locus_tag="ACG8WP_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9029065.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(470..1243)
                     /locus_tag="ACG8WP_002822"
     CDS             complement(470..1243)
                     /locus_tag="ACG8WP_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9029066.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1240..>1853)
                     /locus_tag="ACG8WP_002823"
     CDS             complement(1240..>1853)
                     /locus_tag="ACG8WP_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9029067.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          578 a          469 c          288 g          518 t
ORIGIN      
        1 aaatacaaac aaaaaagcgg aaataacgct catccgcctt cttgtttgtg tttatttcct
       61 cttcaaacgc ttgtctatga ttcagacttt tctctatcgt tattttcatc taaaagttct
      121 tgcattccga taatttctcg atattgatga atcttagtga taccaatgac tttgtctaca
      181 tcaatactaa aacaaatacc attacctgga atatcgatgt tgatgccttg accaatcgca
      241 tccattactt tattcgtcaa actatcgggt actaatgtca acacaatatc tttttctggc
      301 gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac cagaacctct tccatgtaag
      361 atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga taacatcttc gcccattcct
      421 ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta caagatcttt catttgcttt
      481 cccccaattt ttgcaatctt tctttttcga ttctgtcttt ttcatctttt cgtttataaa
      541 tatatcctag tactaaagtg gataatattg gtgtcaatgc aatcattgca accacaccaa
      601 acccgtcaat gatcggatct cttccttcaa tttgtgcagc gattgccacg ctcattgata
      661 aaataaatgt cgaacacatc gggcccgttg ctactccacc gttatcaaaa gccattgcta
      721 aaaagatctt gtccactttt cgtcctaaaa taaatgctag cccgtatcct gggatcaaga
      781 aataataaag agagaatccg acgagcaaac gccacataga caatcctact gctgcgccga
      841 tcccaattga aattaccatc agcatgattt tcgatcggat tctcccctcg ctcaattctt
      901 caacctgttt gatcattaca tgaatcgccg gctcagcaaa ccctactaag aaccccatta
      961 aaaagccaag gggaaccagt atataattgt aaggcaatcc cgcaatagca ctgcctaaat
     1021 gctgtcctac cggtacataa gccatattga ctccgtgcag gaaaaggatc aacccaatag
     1081 tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt tttgcgcata cgaaaactaa
     1141 tgatattcat taaaacaaat aggattacga taggtgccac tgccaaaatg acttccatgg
     1201 taacgatttc gattccatct atcacttgct gccagaaact cattggaaga tcacccccaa
     1261 aatcatcacg gcaagaatcg gccctaacga agcaataccg accattccaa aactgtcatt
     1321 ttcatttttc ttttgtttga tcatactagt catccctccg gcaagggcca agataaatgg
     1381 aacagttact ggaccagtag tcactccccc cgaatcaaaa gcaatcggca taaattcgga
     1441 actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct atagccatca attgataata
     1501 ggatagcttg aatacgaccc gaagcagcgc aaaggctaga aatactccag tgccgacgct
     1561 gacgatccca atcagcaaca ctctgccaat ctctccgtca gatatatcat atacttgttg
     1621 tcccaaaact tgcacggaag gttcagcaat cgtgatcacg atcccgatag caaatcccaa
     1681 actgaccaaa acggtcaaac ttttcttttt gatcatatat ttccctacta gatctccgac
     1741 ttccatcata gaataatctg ctccaaacaa aaagagcgtc attccgatca tcatgattgc
     1801 cgcaccaacc agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa caa
//
LOCUS       DAZKAY010000184         1847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000184 DAZKAY010000000
VERSION     DAZKAY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.193"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG8WP_002824"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG8WP_002824"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9029068.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAZKAY010000185         1752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000185 DAZKAY010000000
VERSION     DAZKAY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.194"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8WP_002825"
     CDS             134..307
                     /locus_tag="ACG8WP_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029069.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG8WP_002826"
     CDS             947..1420
                     /locus_tag="ACG8WP_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9029070.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG8WP_002827"
     CDS             1429..1656
                     /locus_tag="ACG8WP_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9029071.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          588 a          272 c          260 g          632 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tt
//
LOCUS       DAZKAY010000186         1739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000186 DAZKAY010000000
VERSION     DAZKAY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.195"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..656
                     /locus_tag="ACG8WP_002828"
                     /pseudo
     CDS             1..656
                     /locus_tag="ACG8WP_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
     gene            640..1032
                     /locus_tag="ACG8WP_002829"
     CDS             640..1032
                     /locus_tag="ACG8WP_002829"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9029072.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1033..>1739
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8WP_002830"
     CDS             1033..>1739
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8WP_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFP9029073.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          717 a          133 c          298 g          591 t
ORIGIN      
        1 atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa
       61 ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa
      121 ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt
      181 tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata
      241 ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg
      301 aaactattta taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa
      361 gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt
      421 gtaatcatct attgttagat ggcaataata gattaactgg aataattgat tttggagatt
      481 ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa
      541 taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag
      601 caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt gtttattgat
      661 gataatatgg tttttgatat tgataattta aaaggatttc ttaatgatac cagttcattt
      721 gggtttatag ctaaagaaaa taataaaatt ataggatttg catattgcta tacactttta
      781 agacctgatg gaaaaacaat gttttattta cactcaatag gaatgttacc taactatcaa
      841 gacaaaggtt atggttcaaa attattatct tttattaagg aatattctaa agagattggt
      901 tgttctgaaa tgtttttaat aactgataaa ggtaatccta gagcttgcca tgtatatgaa
      961 aaattaggtg gtaaaaatga ttataaagat gaaatagtat atgtatatga ttatgaaaaa
     1021 ggtgataaat aaatgaatat agttgaaaat gaaatatgta taagaacttt aatagatgat
     1081 gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt ttatgatggt
     1141 agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc ttgggaagat
     1201 gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg acaaatatat
     1261 aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga gatagtctat
     1321 ggtatggatc aatttatagg agagccaaat tattggagta aaggaattgg tacaagatat
     1381 attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac
     1441 cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt
     1501 gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa
     1561 tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac
     1621 tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca
     1681 tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaagg
//
LOCUS       DAZKAY010000187         1717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000187 DAZKAY010000000
VERSION     DAZKAY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.196"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            549..881
                     /gene="traE"
                     /locus_tag="ACG8WP_002831"
     CDS             549..881
                     /gene="traE"
                     /locus_tag="ACG8WP_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9029074.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1500..>1717
                     /locus_tag="ACG8WP_002832"
     CDS             1500..>1717
                     /locus_tag="ACG8WP_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9029075.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          583 a          248 c          359 g          527 t
ORIGIN      
        1 cttcttttca ggcaatacga atattattcg cttacatttt tttgtataaa tttagttgtt
       61 ttaaatttaa ttgtctgcta cgcttgtgat gaattaattt tcgttctgca cttttggacg
      121 ataaatttgg acaattgaaa atacaaaaaa aagaaaggcc ctgtgagact cggaaggctg
      181 gtaaccttcg tcgacgtcca caggtggcat taagacaacc tttaatccct taatgcgttt
      241 acctttcagt gttacggtat tacccgcaac ggatttagta taacagatat tcaaaagtga
      301 taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg aatctatgcc
      361 taagcataat gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt
      421 agagggaacg catgtttgga tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat
      481 tctctatttt tgaaaaataa caagaaaaac ttattaaaaa tatttaaagt aagtgggtgg
      541 attattttat gtcatataca ggaattttga gtctcgaaga tatttgtcac tacggtaaac
      601 ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg
      661 tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg atttattacg
      721 ctggtaaaca aaagcaaata attttaaaag atcctatccc tttaaaagaa ctatatccaa
      781 caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa aatggagtct
      841 tgcgttgtac cgcaaaaggg atggggcgtt taatcagctg aattgatcat tctcataact
      901 tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa agaacttaga
      961 attactagaa aaataaggag tagacttgat ctatcaacaa ttcctttcgg ggggtgacgg
     1021 ggtaggtttt tacgattggt aacgtaaaaa attggtcaat cgagtaaatg cccttgtttg
     1081 gatagtggtg aaatgaattt gtgtaaataa tatgcacaga gtaaacaaga gggaatttag
     1141 aagttagaca agttagaata gcgcctagat taatcatgag cagctactaa gggatctagg
     1201 ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag gacgcacaag
     1261 aaattgtgtc gtgtgctgca gtacatgtag atacttgcga ggacgttggt cattggatca
     1321 atcgagcatt taccgatttc tactagggtt tctcgtttcg gacgccataa acgagtcgat
     1381 attttttatg tatttttaag taaacgaaaa acgataaaat atgaacgagt cctgaataaa
     1441 gtgaaaaaat tgatataaaa catagagaaa atgaaattta cgtaatggag gaatgacaaa
     1501 tgaaaaatta tgatccgaat atacgattgg gcacacatac tattaaggtt tctttccaac
     1561 gttgggatta caaaggattt gtgacgttta gaagaggcgg aaattgtaaa ggattagatg
     1621 ttttagcatt agacgaggat gatttatact atcaaaaatt gactgataat ccaattggat
     1681 tcggcttgct acctgaagat gatgaaggta atgaatg
//
LOCUS       DAZKAY010000188         1576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000188 DAZKAY010000000
VERSION     DAZKAY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.197"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1292
                     /locus_tag="ACG8WP_002833"
     CDS             123..1292
                     /locus_tag="ACG8WP_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9029076.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          560 a          255 c          258 g          503 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taataa
//
LOCUS       DAZKAY010000189         1562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000189 DAZKAY010000000
VERSION     DAZKAY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.198"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8WP_002834"
     CDS             144..542
                     /locus_tag="ACG8WP_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9029077.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACG8WP_002835"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACG8WP_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          592 a          228 c          302 g          440 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 ag
//
LOCUS       DAZKAY010000190         1539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000190 DAZKAY010000000
VERSION     DAZKAY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.199"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8WP_002836"
     CDS             complement(52..1518)
                     /locus_tag="ACG8WP_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9029078.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DAZKAY010000191         1525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000191 DAZKAY010000000
VERSION     DAZKAY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.200"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..995)
                     /locus_tag="ACG8WP_002837"
     CDS             complement(120..995)
                     /locus_tag="ACG8WP_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9029079.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1250..1334)
                     /locus_tag="ACG8WP_002838"
     tRNA            complement(1250..1334)
                     /locus_tag="ACG8WP_002838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Leu,seq:caa)
     gene            complement(1352..1423)
                     /locus_tag="ACG8WP_002839"
     tRNA            complement(1352..1423)
                     /locus_tag="ACG8WP_002839"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Gln,seq:ttg)
     gene            complement(1433..1505)
                     /locus_tag="ACG8WP_002840"
     tRNA            complement(1433..1505)
                     /locus_tag="ACG8WP_002840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1470..1472),aa:His,seq:gtg)
BASE COUNT          472 a          314 c          236 g          503 t
ORIGIN      
        1 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
       61 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
      121 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
      181 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
      241 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
      301 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
      361 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
      421 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
      481 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
      541 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
      601 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
      661 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
      721 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
      781 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
      841 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
      901 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
      961 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     1021 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     1081 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     1141 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     1201 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     1261 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     1321 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     1381 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
     1441 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
     1501 ttcgccatca taaaagatgt tattt
//
LOCUS       DAZKAY010000192         1507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000192 DAZKAY010000000
VERSION     DAZKAY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.201"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(478..1092)
                     /locus_tag="ACG8WP_002841"
     CDS             complement(478..1092)
                     /locus_tag="ACG8WP_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9029080.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          492 a          286 c          244 g          485 t
ORIGIN      
        1 aataaacact tataaatatc cattatctat ccttcaattt ttgtgtattt tatttctatt
       61 agaaaaaggt atcctgttgg acaattttgt tatatcaggt aaacttcatt gcaataatga
      121 taacaaatgt actataatta ctttgtaaag agttttgggg aagactcttt catcaacgtg
      181 tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt
      241 tatttttctg acactacata ttcttataac tcatttctca taactgtgaa acaacttata
      301 aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac
      361 taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac
      421 gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta
      481 ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa
      541 aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa
      601 gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg
      661 caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat
      721 taatggatga ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc
      781 ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc
      841 ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact
      901 tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct
      961 agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg
     1021 aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata
     1081 acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct
     1141 aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt
     1201 ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac
     1261 aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac
     1321 aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg
     1381 tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt
     1441 cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat
     1501 acaaaaa
//
LOCUS       DAZKAY010000193         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000193 DAZKAY010000000
VERSION     DAZKAY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.202"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8WP_002842"
     CDS             complement(150..1337)
                     /locus_tag="ACG8WP_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9029081.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKAY010000194         1408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000194 DAZKAY010000000
VERSION     DAZKAY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.203"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..902
                     /locus_tag="ACG8WP_002843"
     CDS             360..902
                     /locus_tag="ACG8WP_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029082.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1058..>1408
                     /locus_tag="ACG8WP_002844"
     CDS             1058..>1408
                     /locus_tag="ACG8WP_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9029083.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          551 a          171 c          270 g          416 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagatcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tctggatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZKAY010000195         1365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000195 DAZKAY010000000
VERSION     DAZKAY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.204"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /gene="spx"
                     /locus_tag="ACG8WP_002845"
     CDS             <1..390
                     /gene="spx"
                     /locus_tag="ACG8WP_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFP9029084.1"
                     /translation="PSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEG
                     GVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDE
                     IRSFVPREIRCVARNLIYLQNNNILNS"
     gene            404..760
                     /locus_tag="ACG8WP_002846"
     CDS             404..760
                     /locus_tag="ACG8WP_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9029085.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          497 a          206 c          255 g          407 t
ORIGIN      
        1 cctagctgta cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt
       61 gaagagaaga atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca
      121 gcgactgaag gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg
      181 ggcatagatt ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct
      241 tcattattga gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa
      301 gatgagatcc ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat
      361 ttacaaaaca ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta
      421 tggatcagcg cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt
      481 gccttatcaa tttgttgact tagaagaacg cgatttaagt acagaagaag cagaagaatt
      541 atgcagtttt gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca
      601 caacattgcg attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca
      661 acataccaat ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata
      721 caatgagcag ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa
      781 gtcactgagc atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg
      841 ttcttttttt gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt
      901 caaatctagg tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat
      961 attcatttct gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa
     1021 atagttgttg acatgttaga aatgacatga taagataact gagttgttaa aaaagaacca
     1081 cttgtgaagc ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg
     1141 ataagttatg ataaatgagt tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta
     1201 aaaaagagtt gacaattact tgtctctctg atatgatata aaagttgcta cggcagccaa
     1261 aaacgaatcg gggaacactc ctgatgaagt agacctttga aaactgaaca aagtaagaca
     1321 aaccaaatgt gtagggcgtc ttgattcaat tcaagacaac aaaca
//
LOCUS       DAZKAY010000196         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000196 DAZKAY010000000
VERSION     DAZKAY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.205"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG8WP_002847"
     CDS             82..>1362
                     /locus_tag="ACG8WP_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9029086.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZKAY010000197         1337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000197 DAZKAY010000000
VERSION     DAZKAY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.206"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG8WP_002848"
     rRNA            <1..1337
                     /locus_tag="ACG8WP_002848"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZKAY010000198         1334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000198 DAZKAY010000000
VERSION     DAZKAY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.207"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1165
                     /locus_tag="ACG8WP_002849"
     CDS             86..1165
                     /locus_tag="ACG8WP_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029087.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAZKAY010000199         1327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000199 DAZKAY010000000
VERSION     DAZKAY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.208"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..996)
                     /locus_tag="ACG8WP_002850"
     CDS             complement(127..996)
                     /locus_tag="ACG8WP_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9029088.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(1136..>1327)
                     /locus_tag="ACG8WP_002851"
     CDS             complement(1136..>1327)
                     /locus_tag="ACG8WP_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029089.1"
                     /translation="KPFSQNFSYYSKLSDFFTTSSLNGVQKLASAVISPTKRKIGGAI
                     FVWLFLLLDAEQLFHNLFL"
BASE COUNT          383 a          267 c          213 g          464 t
ORIGIN      
        1 aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt tctatctcta
       61 gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc gttaagaaag
      121 aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct cactgacgaa
      181 ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt tgtctaagaa
      241 gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt cagtatcgat
      301 catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc
      361 gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga tgccctcttc
      421 ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt ttgttaagtt
      481 agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac tgaatttttc
      541 ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc tttggtcgtt
      601 cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga gcaaaatccc
      661 catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac ggatacgaca
      721 aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt tcagcttttt
      781 gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca tttcacttcc
      841 agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat caaagccaac
      901 aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg ctacactttt
      961 taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc tctacgtcaa
     1021 gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt tgttaaaaaa
     1081 aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa aactcttata
     1141 aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag
     1201 ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     1261 ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     1321 atggctt
//
LOCUS       DAZKAY010000200         1311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000200 DAZKAY010000000
VERSION     DAZKAY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.209"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..>1311
                     /locus_tag="ACG8WP_002852"
     CDS             121..>1311
                     /locus_tag="ACG8WP_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029090.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMN"
BASE COUNT          445 a          166 c          169 g          531 t
ORIGIN      
        1 gaaacactac ttttgtaata taaaaaatat tttattaata ataaaattaa tcacatttta
       61 tatagtgact aatctagtgt ttaaaaaaaa taaatgatac tatatgtaat ataaaaaaac
      121 atgaggtggt atatgaattt gaaaaaaatt ttttctgtaa aaatatttat agtttatgca
      181 tttgttagtt tattatattt atttccagca tttaaaaaca tggaaatatt tggcttttat
      241 gatacggcat ttcacattaa tcgtgcgtta agtttagaat ctatttttag ctcccctatt
      301 aattttgaaa cgtttagatc atatggcatg caagtaaata atttttaccc atggttaact
      361 ctatatcctt tatttttatt gatcaagttt actaatttag caataggcta taaccttttt
      421 ctttatattg ttacgctcat tactctattt atatgtcatt atgtaatgta tgaaattacc
      481 aaaaaacatg ttactagctc ctttttcgct attatatata caacatcttc ttttaggagt
      541 gtagaaatat ttcttcgagg ggcaatgggg gaattactcg caatgtcaat acttcctctt
      601 attttactcg ggtttataaa gctttacgac tcaaaaaaag aatcttgggt tatgttggcg
      661 attagcatga ctttattaat ttacactcat gttctttctg tagctatgac tatggtaatg
      721 attataattg ctttaattat tcaattttat cggaaaaaga aaatagaaat cttcttaatt
      781 attaaattaa taaaagctgc tatagttact ttacttcttt cactagcgtt tataggtcct
      841 atgattgagc agacattgta tcaagatctg aatagacctt atgtggatat attacaaaaa
      901 agagcaattt atctgggtaa agagtttttg attggaagta tagattcaga attactatca
      961 tttggtattg gcttaacttt gcttatttgt ttaattatac taacatttaa ctttaaaaaa
     1021 ttaggaatct tatctaaaat cacttatata atgggtataa tatctattct actttgtaca
     1081 aaaatatttc catggtttgc cttacaaaat actcctttaa atattattca agctccttgg
     1141 cgttttatga tattttcaac attgttcata tctttttcag tatctctatt tatttctcca
     1201 cagttggaaa aattttctta tacaaggaga agaaatttta tactattatt aatattagct
     1261 atttcaattt taaattattc atcagtagtt aacatggtga aagaaatgaa t
//
LOCUS       DAZKAY010000201         1266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000201 DAZKAY010000000
VERSION     DAZKAY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.210"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8WP_002853"
     CDS             complement(31..1203)
                     /locus_tag="ACG8WP_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9029091.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          344 a          263 c          243 g          416 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacat
//
LOCUS       DAZKAY010000202         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000202 DAZKAY010000000
VERSION     DAZKAY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.212"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8WP_002854"
     CDS             414..947
                     /locus_tag="ACG8WP_002854"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9029092.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKAY010000203         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000203 DAZKAY010000000
VERSION     DAZKAY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.213"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8WP_002855"
     CDS             <1..1076
                     /locus_tag="ACG8WP_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9029093.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZKAY010000204         1152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000204 DAZKAY010000000
VERSION     DAZKAY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.214"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1077)
                     /locus_tag="ACG8WP_002856"
     CDS             complement(<1..1077)
                     /locus_tag="ACG8WP_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029094.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENP"
BASE COUNT          306 a          257 c          185 g          404 t
ORIGIN      
        1 gggattttca ccaaattctt cggttattct ttcttcccag gattttggtc ttaaggcctc
       61 attcagattg ataaacagct tttttactcg tttctgatac tccttcatga cctttttcat
      121 taatgttttg attctacgac tatagattcc atagcgacga atcgttttga aattcttatt
      181 ttgaatatgg cgaatcagtg cagctatgaa ttcgtctata ctcatggtca tcgtctcttt
      241 agtattcgtt cgtttgtcca tgtagttgaa catgacagtg tcgccatcat acattaagat
      301 tcggcttaat gcgatcgggc cacgcttcat ataacgactg atgtattgga gtagtgactt
      361 tagatttgtc ctgctccttt taggagcatt tacgtaaaac ccttctccat tgttcttgta
      421 cgcagcttgt aatcttgatt gaactctttg cttgtcccac tcacctaacg ttcgtctaat
      481 tagtttcaat acagcatttt tccagtattt tcttaacata gaaaaaggta aataatcgta
      541 ttcttcccaa cttccatctc ttgttaagcc acccatagta acaaccatat gaacatgcgg
      601 attaaattct agttttgatc caaaggtgtg gattgtagct actatacctg ccttcacctt
      661 tcttttctca aaataggcaa gaataatttt tgcggcttca tccattagtc ctttcagtaa
      721 ggaatctcga tagttttcca tcataaagat ttcccgtaac ccctcatcga tcgtaaagat
      781 tacgtgccga tgtgtcactt taaacatatc attcgccaca accgctgacc acctttggct
      841 ttcacctgtt gcacacgttg gacagaactt tccacgacat ttcaagggaa caaactttgt
      901 ttgatgacat ccctcgcaga caaacagtcg gtatcctttt tgaatatctc cacaatatct
      961 aaacttgttt acttcccgtt tgacgactag ccgaatcttt cgcccgtatt tctcaacaaa
     1021 gttatcccaa ttatggtgtt cgtcaaaaaa gattgcttcg agaatattcg ctttcatagt
     1081 aatattttac ttgttcaaaa acaaaatgaa aagggctaaa agactggtag tgcagccttt
     1141 ttagccctta ac
//
LOCUS       DAZKAY010000205         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000205 DAZKAY010000000
VERSION     DAZKAY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.216"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8WP_002857"
     CDS             <1..1087
                     /locus_tag="ACG8WP_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9029095.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAZKAY010000206         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000206 DAZKAY010000000
VERSION     DAZKAY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.217"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>1063)
                     /locus_tag="ACG8WP_002858"
     CDS             complement(38..>1063)
                     /locus_tag="ACG8WP_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP9029096.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DAZKAY010000207         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000207 DAZKAY010000000
VERSION     DAZKAY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.218"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..127
                     /locus_tag="ACG8WP_002859"
     tRNA            38..127
                     /locus_tag="ACG8WP_002859"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="ACG8WP_002860"
     tRNA            142..213
                     /locus_tag="ACG8WP_002860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="ACG8WP_002861"
     tRNA            229..301
                     /locus_tag="ACG8WP_002861"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="ACG8WP_002862"
     tRNA            311..383
                     /locus_tag="ACG8WP_002862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="ACG8WP_002863"
     tRNA            419..494
                     /locus_tag="ACG8WP_002863"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
     gene            496..576
                     /locus_tag="ACG8WP_002864"
     tRNA            496..576
                     /locus_tag="ACG8WP_002864"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Tyr,seq:gta)
     gene            583..656
                     /locus_tag="ACG8WP_002865"
     tRNA            583..656
                     /locus_tag="ACG8WP_002865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
     gene            659..731
                     /locus_tag="ACG8WP_002866"
     tRNA            659..731
                     /locus_tag="ACG8WP_002866"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:His,seq:gtg)
     gene            741..812
                     /locus_tag="ACG8WP_002867"
     tRNA            741..812
                     /locus_tag="ACG8WP_002867"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:773..775,aa:Gln,seq:ttg)
     gene            858..928
                     /locus_tag="ACG8WP_002868"
     tRNA            858..928
                     /locus_tag="ACG8WP_002868"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:890..892,aa:Cys,seq:gca)
     gene            949..1033
                     /locus_tag="ACG8WP_002869"
     tRNA            949..1033
                     /locus_tag="ACG8WP_002869"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:983..985,aa:Leu,seq:caa)
BASE COUNT          239 a          247 c          281 g          292 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc accatggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
      541 ccttagggtt cagtggttcg aatccactct cctccatttt ataggggcat agtttaaagg
      601 tagaacagcg gtctccaaaa ccgttagtgt gggttcaatt cctgctgccc ctgccatggc
      661 gaatgtggcg aagtggttaa cgcaccggat tgtggctccg gcattcgtgg gttcgattcc
      721 catcattcgc cctttattct tggggtatag ccaagcggta aggcaaggga ctttgactcc
      781 ctcatgcgtt ggttcgaatc cagctacccc agtttctata atttattctt tattttatag
      841 attgataata gtataatggc ggtatagcca agtggtaagg cagaggtctg caaaaccttc
      901 atcaccggtt caaatccggt taccgcctta gtactaataa atcaacttgc cggcgtggcg
      961 gaattggcag acgcgctgga ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc
     1021 ccggccgccg gtattgacta agatgtcttc atcttagtt
//
LOCUS       DAZKAY010000208         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000208 DAZKAY010000000
VERSION     DAZKAY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.219"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8WP_002870"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8WP_002870"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9029097.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZKAY010000209         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000209 DAZKAY010000000
VERSION     DAZKAY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.220"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>1003
                     /locus_tag="ACG8WP_002871"
     CDS             15..>1003
                     /locus_tag="ACG8WP_002871"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9029098.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLHT"
BASE COUNT          301 a          210 c          190 g          302 t
ORIGIN      
        1 gcaagaagat ctctatgatt ctcctgaatc gtagcggtaa taaacggacc tatcttcggt
       61 tgaagaaaca gcgatacctc tgccgagcat gtaaaaaata ttttaccgct cggacgtatc
      121 tcgttacccc attctgcttt atttctaagc agattcatta caagatctta gaagaattga
      181 cagagcgtca atctataaaa gcaattggta aacattgcga tgtttctgtg acaactgttc
      241 agcggacatt gtccgccttg tcatcagata tccgtcagaa tcttgagtat ttacctcggt
      301 gtctattatt cgatgagttt cgttctcttt ctacatggca cggtaaattt tctttttctt
      361 gtatggacgg tgagaccggg aaactgtttg agatcttacc tagccgccgt aaaaaagatc
      421 tcgttgcttt tttcatgcgt ttttctctca aagcgaggtt gggtgtacat tacatcgtaa
      481 cggatatgaa cgctccttat ttctcactag ttaaagagtg ttttccaaat gcacaagtga
      541 tcgtcgatcg gtttcacatt gttcaacatc tgaaccgctt cttcgatgcg gttcgaaaac
      601 gtgtgatgaa aacacttgat caaaaagacc caatacaagc taaacagtat cgccaattga
      661 aatccttatt caaattactt ttaaaatcag aggatcgttt agattacaat acactaacga
      721 aaagacgcaa tttcaattgg agactattga ctgaaacaga agtagttgac cgccttctaa
      781 agatttctcc tgaacttaaa acagcttatc gttactacca agacctgctt actgcgtatc
      841 aggaaaaaga tccagatact tttttccagc tgcttcgagc aatgcctgga gaagttccat
      901 tggaactgca ccatatcaaa aaagcctttt tcaaatacga gaaagggatt cggctggcat
      961 tactgaagaa gtattcgaat gcacgactgg aaaatctcca tac
//
LOCUS       DAZKAY010000210         1000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000210 DAZKAY010000000
VERSION     DAZKAY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.221"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACG8WP_002872"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACG8WP_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAZKAY010000211          998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000211 DAZKAY010000000
VERSION     DAZKAY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.222"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..634)
                     /locus_tag="ACG8WP_002873"
     CDS             complement(80..634)
                     /locus_tag="ACG8WP_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9029099.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DAZKAY010000212          991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000212 DAZKAY010000000
VERSION     DAZKAY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.223"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG8WP_002874"
     tRNA            complement(21..94)
                     /locus_tag="ACG8WP_002874"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG8WP_002875"
     tRNA            complement(108..181)
                     /locus_tag="ACG8WP_002875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG8WP_002876"
     tRNA            complement(209..281)
                     /locus_tag="ACG8WP_002876"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG8WP_002877"
     tRNA            complement(301..374)
                     /locus_tag="ACG8WP_002877"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACG8WP_002878"
     tRNA            complement(384..457)
                     /locus_tag="ACG8WP_002878"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACG8WP_002879"
     tRNA            complement(468..553)
                     /locus_tag="ACG8WP_002879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACG8WP_002880"
     tRNA            complement(570..641)
                     /locus_tag="ACG8WP_002880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACG8WP_002881"
     tRNA            complement(653..725)
                     /locus_tag="ACG8WP_002881"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACG8WP_002882"
     tRNA            complement(743..824)
                     /locus_tag="ACG8WP_002882"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACG8WP_002883"
     tRNA            complement(832..904)
                     /locus_tag="ACG8WP_002883"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACG8WP_002884"
     tRNA            complement(908..980)
                     /locus_tag="ACG8WP_002884"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAZKAY010000213          956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000213 DAZKAY010000000
VERSION     DAZKAY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.224"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          365 a          109 c          181 g          301 t
ORIGIN      
        1 ttcttggcta cttttttatt tttatgtatc tacaaaagtt tccaggggca gaacctgtga
       61 ttattaatgg aagaacctca agtattgtct taaatcattg ttatgaaaat ggattaaaag
      121 agttcatcaa ggtgtgatta acaagttact tttcattaca tcgaattttt cagaagttgc
      181 ttatattggt gatgatatta atgatttatc atgtatgaca tctgtgaaga agcgggagga
      241 atagtagaat gtccaaaaaa tgccacaaga aatgagattg aattatctga ttttattgta
      301 actcacagtg gtggtgacga atctgtgata gagtttattg agtggatatt ggaatcgata
      361 tagtgtagta gtacagaaaa attatcatac aattaatact aggcataaga atattggcat
      421 attctagagt taaaagtaac tattcgaaaa ggataaggtt agaatttttt gttttgccag
      481 tggaagaata taaaaagggc attggaaatt aatatattag aatgctaagc ggagaaaaaa
      541 atatatgaaa agtacgtcct tgtgatggga tggaaaaata cacttatttc aaacaacgat
      601 aatataatta tgtgaaccga agtatactcg cttctaattc aatcctcaaa tcccgacagt
      661 cgcgacttca aaaaagcgta tagttttaat gtatccccat atgatccgaa aaatggtaat
      721 ttacaattaa cagtttgatg atgttgaaga tgtggaaatg ctgaaattac catgatgaga
      781 aacacattaa ttaaagggaa ttaattaact agaatataat ctgattcaat ttgaattaag
      841 caaaaaaagg ctgagtttta aataatcgcg aattgtaaaa ttaagttaga aaaataaaaa
      901 ggcatttatg gtacactcaa attgtatttc cgacgaaaga aaacaaagga gtgtaa
//
LOCUS       DAZKAY010000214          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000214 DAZKAY010000000
VERSION     DAZKAY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.225"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8WP_002885"
     rRNA            <1..>943
                     /locus_tag="ACG8WP_002885"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKAY010000215          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000215 DAZKAY010000000
VERSION     DAZKAY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.226"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            625..855
                     /locus_tag="ACG8WP_002886"
     CDS             625..855
                     /locus_tag="ACG8WP_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029100.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          338 a          145 c          148 g          307 t
ORIGIN      
        1 tgcttcacga taaaaactcc taatagttat ttagatagaa gcctatcaca tcaacttttt
       61 agtagaaaag tacatgtttt gtcttttttg ctaatagctg accctttgag aaacttgggt
      121 tcaaaagttg ctaaattcgg taaatgtttt ggtatttttc gtattatatt tgtcgtcaag
      181 ctgactacta aattttgccc tattttgacg acactgatag tcaatgtcgg acaatctagg
      241 acaattttta agggaatatg attgaaaaat ggtccaaaac agccaaaaat ggcttaaaac
      301 tgtgatagag aaaaaagaaa aagcccttga tatacaagga cttttcacaa atttcagatt
      361 tccaaggaag ttttccgtgt ttatcttctg atttctttga tacgagcagc ttttccgtgt
      421 aatgcacgta aacaaaacca ctatattcta ttatgtatct aataccctca aaacagctat
      481 aaatcaacaa taccgacata taataaacac tatgttcaat ctgagatata tcaaagataa
      541 acgtattttt gccccctttt tgcccctcaa atatcgcttt acaacgagaa cgtaagttcg
      601 tataatcgtt ttgaggtgat ttttatgatg gaagacttta ttagaaaaaa tatcagtgat
      661 gaatatgcag acttttatga gcaaagcagc aaaaaagata aattccagat ggatgtttca
      721 attttagcta tattagcttt ttccgaaaat aaacaacctg taactgcaaa aaaagaaaca
      781 gtattctctg aaggcaaaat aaaaactcga tatatattag aggtagaaac taattttaaa
      841 aacagatcgg agtaatggtt atgcttttta atgagacaca attatggttt aaattcgatc
      901 ctacgaatag atttatcaaa gatttttata aagtatgg
//
LOCUS       DAZKAY010000216          906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000216 DAZKAY010000000
VERSION     DAZKAY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.227"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACG8WP_002887"
     CDS             <1..720
                     /locus_tag="ACG8WP_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9029101.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DAZKAY010000217          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000217 DAZKAY010000000
VERSION     DAZKAY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.228"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..>877)
                     /locus_tag="ACG8WP_002888"
     CDS             complement(135..>877)
                     /locus_tag="ACG8WP_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9029102.1"
                     /translation="QVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLV
                     YFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRF
                     TIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQ
                     VLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHV
                     LFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          334 a          152 c          103 g          288 t
ORIGIN      
        1 cactatatct tctagcgttt tcaaattcag aataaataaa taaatctcct tttgattctc
       61 ggtaaaaatt ttgataaaca tttaatatat taatcaaatg taaaggagtc caagcaatga
      121 aagctgtctt catattaatt ctcctttttt aaatacaagt aagaaatgct tcccaaataa
      181 taccagaaaa caattgaaat gacatcgcgc ttattgaaaa gtacatgagc ctccaccatt
      241 tcatctataa aaatagatag tataaaaatt aaaacaatac caagaaacgc ataattcact
      301 ttacttctaa aaaatgtttt gagtaaacta aagatattta acacatagaa agcatagata
      361 catataaaag caattatacc acttgataca agtacttgaa gaaatccatt atgcatgttt
      421 ccatgtgctc tttttagttc attcacatct gtagtatcaa gatctacttt tttcaaaaat
      481 tttgcatcgc tattcccata gaaatcggtc tctccataac caaataacaa agttggtctt
      541 ataactttaa gcgcagcttt ccaaatcgta aaacgaccag ctgaaatttc ggagttatca
      601 ctagaatgct gtaatattac ttctttttct ggaagtggag ttttatctga ttcatttgac
      661 tctataactt ctacttttct atcaatcgat acaggaattg ttgctaaaaa atccttagca
      721 taattagtac caaaagaaaa atataccaat agaactaata atatgcttac catccatttg
      781 atttttacag gtagtacttc tctaatgctc tttctaaatt ttgaattggc aaacaacaaa
      841 agaaataata ctaaaaaaga acaaattact acctggg
//
LOCUS       DAZKAY010000218          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000218 DAZKAY010000000
VERSION     DAZKAY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.229"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..877
                     /locus_tag="ACG8WP_002889"
                     /pseudo
     CDS             1..877
                     /locus_tag="ACG8WP_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
BASE COUNT          303 a          165 c          160 g          249 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc atgcggtatg
      301 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
      361 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
      421 atccatgatc gacctgataa ttatcctcat ttcaattctg agtttggtca ccttgaaggt
      481 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
      541 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
      601 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgct attacgtttg
      661 actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat
      721 attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct aacgggattc
      781 tgcgtcgtaa tggactgccg aaatcaatgg attttagaga agtgaatcag acatttattt
      841 ccagtgtcag caatcaacgt aatcatattc caagaaa
//
LOCUS       DAZKAY010000219          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000219 DAZKAY010000000
VERSION     DAZKAY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.230"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>822
                     /locus_tag="ACG8WP_002890"
     CDS             56..>822
                     /locus_tag="ACG8WP_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9029103.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIG"
BASE COUNT          234 a          164 c          161 g          263 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc ag
//
LOCUS       DAZKAY010000220          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000220 DAZKAY010000000
VERSION     DAZKAY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.231"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8WP_002891"
     CDS             <1..375
                     /locus_tag="ACG8WP_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029104.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
     gene            389..790
                     /locus_tag="ACG8WP_002892"
     CDS             389..790
                     /locus_tag="ACG8WP_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029105.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          296 a          108 c          150 g          267 t
ORIGIN      
        1 gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa tccagctctt
       61 tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc tgtggtgtct
      121 gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa caagaatgaa
      181 aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt taacgatata
      241 aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca ttatgctgtt
      301 gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt caacaaacca
      361 gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa atacacatcg
      421 gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa ttctagcatc
      481 tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc aacttgtaat
      541 tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt ttgatgtgat
      601 aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag atttaaactt
      661 gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg atgttttgga
      721 tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag tagcaacttt
      781 gggggaataa aatgtttgac tttaatgaat atttatctat t
//
LOCUS       DAZKAY010000221          791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000221 DAZKAY010000000
VERSION     DAZKAY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.232"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8WP_002893"
     CDS             <1..716
                     /locus_tag="ACG8WP_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9029106.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAZKAY010000222          780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000222 DAZKAY010000000
VERSION     DAZKAY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.233"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>780
                     /locus_tag="ACG8WP_002894"
     CDS             <1..>780
                     /locus_tag="ACG8WP_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9029107.1"
                     /translation="VLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVS
                     ADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIE
                     NKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPF
                     DLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATH
                     DPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDI"
BASE COUNT          318 a           86 c          140 g          236 t
ORIGIN      
        1 aagttctatt tgttaaaaat agaactgtaa aaactacaac tattcagttg ttaaaaactg
       61 aaaacaataa cgcattaagg aaatttgaca tttttaatag ttcgattgaa gttccctata
      121 tcgaaaaggt aagtgcagat gtattaaaag ttgaagaaat tacttatgaa aatattctaa
      181 aagacataaa ttttgatgtc aaagttggag atgttattgg tttggtaggg aaaaacggtg
      241 tggggaaaac aaccttacta agaatactca tgaagattat tcgccctacc aaaggaaaga
      301 taatcgaaaa taaaaaaaat ttgtcatcgc cgtttttagt aatgcaagaa atggattacc
      361 aatttttcac agaatcagtg aggagcgagt tagccctggg gaatgaaata gttagtaaag
      421 atcagcaaga aaaaatttta aaaaaaatgg aactttataa aatgaaagat caattaccgt
      481 ttgatttatc cggtggagaa aaacaaagat tattggtttc tatagcttca ttgtcaaaaa
      541 ctaatttatt tctatttgat gaacctacaa gtgggttgga ttatattaat atggaaagga
      601 ttgctgacct tattaaagac ttaaaagaaa agggtgcagt agttatagcc actcatgatc
      661 cggaatttct ttataaaaca tgcaatagaa ttatttattt aaaaaatggt ggaataaaaa
      721 aagatataaa acttagagta gaagattcaa ctttgataga acagattttt aatgacataa
//
LOCUS       DAZKAY010000223          691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000223 DAZKAY010000000
VERSION     DAZKAY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.234"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          265 a           86 c           80 g          260 t
ORIGIN      
        1 aaaatatttt tattaatatt ttataacttt acgcaagatc ataattttgt cgtgtagata
       61 tttcattata tcactaaaga taataagatt taatgacctc attaatattg tttgctaggt
      121 ccaaactctt attaaacttt ctttgctata aaacccaaac atagtaataa aagaataact
      181 tattaccttt aaattatact accaacaaat ggtagtatat tattaattta tataatatga
      241 aacctaaaat atagtagtat gttaatttat actaaattaa ataaagtact acctttaata
      301 ggtagtactt tattatcaag ccattattcc ttcttctact agttcttttt gtcacataat
      361 tttgcaatca ataaatgttg cgcaataagt ataagaattc cattttctaa atttgaaagg
      421 tgatccataa tttcatgttc aagtagttta ttaattgtcc acttgtcgct tcatctaaaa
      481 attttatagg ttttaggaat gctattcgct tattttagga agattttaac tgtttgctgt
      541 agatatatat catactagta tcgtaataaa agtactctaa aagaagaatt ttcaaatacg
      601 caaaattaag tacgaaagag ttgaaacaaa tttggagaaa aatgagcata gaatgtctaa
      661 tgagttaaat agacctaaaa attatctaaa a
//
LOCUS       DAZKAY010000224          653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000224 DAZKAY010000000
VERSION     DAZKAY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.235"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..326
                     /locus_tag="ACG8WP_002895"
     CDS             123..326
                     /locus_tag="ACG8WP_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFP9029108.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            326..>653
                     /locus_tag="ACG8WP_002896"
     CDS             326..>653
                     /locus_tag="ACG8WP_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9029109.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSY"
BASE COUNT          270 a           63 c          111 g          209 t
ORIGIN      
        1 caatcattgt tatataataa aatgttatat aataaaatta ctaatgatat ttaatattac
       61 aagcaatctt aagaaattcg aaaaggaaaa aatgattatg aaaaagaaag aaaaagaaag
      121 tattgaaaca ttgtgttatt ctaggaatat taggaacttg tctagctggc attggtacag
      181 gaatagacgt tgacgcagct acttactatg gaaatggtct ttattgtaac aaagaaaaat
      241 gttgggtaaa ttggggacaa tcatggtcag aagggttaaa acgttgggga gataatcttt
      301 ttggtagttt tagtggagga cgataatgac ttcaataaac attgatcaca caaaaaaagt
      361 catacatgaa ttatacaata gtttaatgac acgacctaat cagacgaaag cattattaga
      421 tattactgat gttttatttc aggtatataa aaaaattgat acagcaaaat atcctgaaat
      481 tcttgtaaat aaattagtta attatattta catcgtgggt tttgataata aaatacgctt
      541 tattgataat gatgaaaaat tattaataga attaggggat atttctaaaa aagctggaat
      601 taatagtaaa tacaaagcaa actttacaga taaatcgcaa ttttatagtt att
//
LOCUS       DAZKAY010000225          649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000225 DAZKAY010000000
VERSION     DAZKAY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.236"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACG8WP_002897"
     CDS             <1..>649
                     /locus_tag="ACG8WP_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029110.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAZKAY010000226          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000226 DAZKAY010000000
VERSION     DAZKAY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.237"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          229 a           71 c          139 g          182 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc t
//
LOCUS       DAZKAY010000227          615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000227 DAZKAY010000000
VERSION     DAZKAY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.238"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..>615)
                     /locus_tag="ACG8WP_002898"
     CDS             complement(70..>615)
                     /locus_tag="ACG8WP_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9029111.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          176 a          116 c          108 g          215 t
ORIGIN      
        1 acccccaaaa gttagacttt tttggagtgg agaaatccac tccttttttc atgctctaaa
       61 atgatatttt tacacaactg ttacggttgt tttacgaaat tggacgggac tttgccagtt
      121 taatttctgt tttatccgtt cattattgta gtaatagata tagctatcga tggctgattt
      181 taattcctcg aaacagttat aaactttccc gtgaaaaatc tcttgtttta ataagccaaa
      241 aaaattctcc ataagtgaat tatctaagca gttccctttc cgagacatgc tttgaaagat
      301 tttgtactcc ttcaatttgt ttctataagc tttcatttga tacgcccaac cttgatctga
      361 gtgaaacgta cgacgatagg gacaatcgtt agtggtctgg atagcttcct ccaaaccttc
      421 cattaccgct aatgcattgg gcttttcaga gatacgatag gataaaatct cactattgaa
      481 catatccata aaaggatcta gatacaattt cttttgacgg ataatgcctt tggcatccac
      541 ttcatggtat ttaaattcag ttgtatccgt cgtgattttc tgatggcaga tactggtgta
      601 gaaacgacga tggat
//
LOCUS       DAZKAY010000228          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000228 DAZKAY010000000
VERSION     DAZKAY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.239"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          185 a           81 c           81 g          226 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttt
//
LOCUS       DAZKAY010000229          550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000229 DAZKAY010000000
VERSION     DAZKAY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.240"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>550
                     /locus_tag="ACG8WP_002899"
     CDS             <1..>550
                     /locus_tag="ACG8WP_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9029112.1"
                     /translation="TISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILST
                     GQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKV
                     MNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKA
                     DWYGRKIIKVDKWFPSSQICSN"
BASE COUNT          203 a           87 c           94 g          166 t
ORIGIN      
        1 ctacaatatc acgtcattct agcggtaaat attatatttc tttgttatgc aaagaagaag
       61 ttcgcgaact acctaaatct aattctgctg ttggtattga cctaggtatt atagactttg
      121 caattctttc taccggtcaa aaatttgata ataataaatt tacgtccaaa atggaaaaga
      181 aactcaaacg tgaacaacgt aagttgtcaa gacgtgcctt attagctaaa caaaaaggta
      241 tcaatctgtt tgaagctaga aattatcaaa cacaaaaacg taaagtagca cgattgcatg
      301 aaaaagtaat gaatcaacgt actgattttc tgaataagtt gagtacagaa attatcaaaa
      361 atcacgatat catctgtatt gaagacttaa acacaaaagg catgttgcgt aatcataaac
      421 tagcaaaaag tatttcggat gtttcttggt ctagttttgt gtctaaatta caatacaagg
      481 ctgactggta cggtcggaaa atcatcaaag tagataagtg gtttccatct agccaaatct
      541 gttcgaattg
//
LOCUS       DAZKAY010000230          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000230 DAZKAY010000000
VERSION     DAZKAY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.241"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>537
                     /locus_tag="ACG8WP_002900"
     CDS             <1..>537
                     /locus_tag="ACG8WP_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9029113.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTT"
BASE COUNT          185 a           96 c          104 g          152 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccac
//
LOCUS       DAZKAY010000231          536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000231 DAZKAY010000000
VERSION     DAZKAY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.242"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..536
                     /locus_tag="ACG8WP_002901"
                     /pseudo
     CDS             1..536
                     /locus_tag="ACG8WP_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          233 a           71 c           93 g          139 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
      241 ttagaaacct tgattcaaga acaagatttg agcaagaaga aatcaaggtt aaaacaatcc
      301 ttcagctcaa aaaagaattt aaattgaatc tattactttc aattgcacaa ttagcgaagt
      361 caacttatta ttactgggta aaaaaattag ataagccaga taaatatagc aaggtaaagc
      421 aagaaattac agcgattgta aaagaatcaa gaaactctta tggttatcgt agagtcactt
      481 tagcgttaaa gatgaaagga tatacaatca atcataaaac agttagaaaa ttaatg
//
LOCUS       DAZKAY010000232          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000232 DAZKAY010000000
VERSION     DAZKAY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.243"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>526
                     /locus_tag="ACG8WP_002902"
     CDS             <1..>526
                     /locus_tag="ACG8WP_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9029114.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLK"
BASE COUNT          229 a           63 c           86 g          148 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagtt
//
LOCUS       DAZKAY010000233          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000233 DAZKAY010000000
VERSION     DAZKAY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.244"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>513)
                     /locus_tag="ACG8WP_002903"
     CDS             complement(107..>513)
                     /locus_tag="ACG8WP_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9029115.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DAZKAY010000234          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000234 DAZKAY010000000
VERSION     DAZKAY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.245"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..512)
                     /locus_tag="ACG8WP_002904"
                     /pseudo
     CDS             complement(1..512)
                     /locus_tag="ACG8WP_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          197 a           80 c           64 g          171 t
ORIGIN      
        1 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
       61 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
      121 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
      181 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
      241 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
      301 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
      361 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
      421 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
      481 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZKAY010000235          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000235 DAZKAY010000000
VERSION     DAZKAY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.246"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>497
                     /locus_tag="ACG8WP_002905"
     rRNA            <1..>497
                     /locus_tag="ACG8WP_002905"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          120 c          147 g          111 t
ORIGIN      
        1 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct
       61 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct
      121 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
      181 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
      241 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc
      301 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc
      361 cgcaacgagc gcaaccctta ttgttagttg ccatcattca gttgggcact ctagcaagac
      421 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
      481 tgggctacac acgtgct
//
LOCUS       DAZKAY010000236          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000236 DAZKAY010000000
VERSION     DAZKAY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.247"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>495)
                     /locus_tag="ACG8WP_002906"
     rRNA            complement(<1..>495)
                     /locus_tag="ACG8WP_002906"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           99 a          162 c          106 g          128 t
ORIGIN      
        1 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
       61 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      121 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      181 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      241 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      301 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      361 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      421 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
      481 cccgaaagcg ccttt
//
LOCUS       DAZKAY010000237          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000237 DAZKAY010000000
VERSION     DAZKAY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.248"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>493)
                     /locus_tag="ACG8WP_002907"
     CDS             complement(54..>493)
                     /locus_tag="ACG8WP_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029116.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DAZKAY010000238          492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000238 DAZKAY010000000
VERSION     DAZKAY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.249"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /locus_tag="ACG8WP_002908"
     CDS             <1..325
                     /locus_tag="ACG8WP_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9029117.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          196 a           66 c           90 g          140 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta ta
//
LOCUS       DAZKAY010000239          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000239 DAZKAY010000000
VERSION     DAZKAY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.251"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8WP_002909"
     tRNA            complement(20..91)
                     /locus_tag="ACG8WP_002909"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8WP_002910"
     tRNA            complement(113..200)
                     /locus_tag="ACG8WP_002910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8WP_002911"
     tRNA            complement(225..298)
                     /locus_tag="ACG8WP_002911"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8WP_002912"
     tRNA            complement(312..382)
                     /locus_tag="ACG8WP_002912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8WP_002913"
     tRNA            complement(384..459)
                     /locus_tag="ACG8WP_002913"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKAY010000240          447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000240 DAZKAY010000000
VERSION     DAZKAY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.252"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..256
                     /locus_tag="ACG8WP_002914"
     CDS             164..256
                     /locus_tag="ACG8WP_002914"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029118.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT          173 a           63 c           92 g          119 t
ORIGIN      
        1 atctcttgtt gaattctagt aatgaagcga ccctaaaact tagaccaaaa aatctaactt
       61 ttgggtggag ttgtcaagaa aaaccgggca cttagttaaa aaataagttg gacgggttat
      121 actaaaattg acaccaaatt aagagaaagc gaagatctgg attatgccaa gacgttcatt
      181 tgatgaaata tttaaaatta ccgctgtaaa actggtaacg gaagaagggt tttctgttaa
      241 agaagttagt ctatagctag aagctcgctc taatagcgtg tattgatgag ttcaagaagt
      301 tgaaaaatat ggataacgta cttttctcgg tagaggaagt catgaaatcc attcgcagaa
      361 atgaaattac acgcttagaa aaagagaata aacggttaaa agaataggta gaactcctaa
      421 aaattaccga agtcttcctg aaagaaa
//
LOCUS       DAZKAY010000241          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000241 DAZKAY010000000
VERSION     DAZKAY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.255"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>423
                     /locus_tag="ACG8WP_002915"
     CDS             <1..>423
                     /locus_tag="ACG8WP_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9029119.1"
                     /translation="KNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQL
                     TFPELEQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQK
                     ADQIIHYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT          149 a           99 c           68 g          107 t
ORIGIN      
        1 aaaaatcaac gtcagccaaa agaagtacaa agcaattttt acttagagat gcgcgattta
       61 gcccgttttt atcaagaaat tgagaagaaa atcactcgtc tgcgcatgga tttacacaac
      121 tgccttcaat tgacttttcc agagcttgaa cagttctttt caaacaggct gaccccttac
      181 gcattgacat tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca
      241 aaaataaaaa ataaactgat caacgaaaca cggaaaaaaa tctccgcgaa tcgtgccgag
      301 caaaaggcag atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac
      361 agtatccact gtcaaaaaac gatctattac gccgaacttc tccaagattt actcgaacaa
      421 aaa
//
LOCUS       DAZKAY010000242          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000242 DAZKAY010000000
VERSION     DAZKAY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.256"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /locus_tag="ACG8WP_002916"
     CDS             <1..216
                     /locus_tag="ACG8WP_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP9029120.1"
                     /translation="YEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQKEFRQQY
                     EEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            233..385
                     /locus_tag="ACG8WP_002917"
     CDS             233..385
                     /locus_tag="ACG8WP_002917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029121.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
BASE COUNT          164 a           56 c           95 g          105 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
//
LOCUS       DAZKAY010000243          408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000243 DAZKAY010000000
VERSION     DAZKAY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.257"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8WP_002918"
     CDS             <1..390
                     /locus_tag="ACG8WP_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029122.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DAZKAY010000244          408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000244 DAZKAY010000000
VERSION     DAZKAY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.258"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>408
                     /locus_tag="ACG8WP_002919"
     CDS             <1..>408
                     /locus_tag="ACG8WP_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029123.1"
                     /translation="KLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDIL
                     YAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNN
                     HIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          139 a           84 c           71 g          114 t
ORIGIN      
        1 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
       61 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
      121 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
      181 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
      241 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
      301 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
      361 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaat
//
LOCUS       DAZKAY010000245          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000245 DAZKAY010000000
VERSION     DAZKAY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.261"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..207
                     /locus_tag="ACG8WP_002920"
     CDS             <1..207
                     /locus_tag="ACG8WP_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9029124.1"
                     /translation="AILAALMALSLISRQILYYSFCDVNKHSNYEIGQLIRGYGEKNV
                     EKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT          128 a           49 c           95 g          118 t
ORIGIN      
        1 gcgattttag cggctttgat ggcgcttagt ctgattagcc gtcagatact ctactacagc
       61 ttctgtgacg taaacaagca ctctaattat gaaatagggc aattgatacg aggatacgga
      121 gagaagaatg tagagaagct gaaatccatc gcattgatcg aatttgctga agcatacaaa
      181 aaaggcgtgt tagttcagta tcgttaattt tgtagggttt ttgtagggat agtgtagggt
      241 ttttgagcgg tttaacgtga tattatgata gtgtcgaaag attagtgata ggtctaagac
      301 aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct tcgatagaca
      361 gcagcggaaa tattaagaat aaggatgtga
//
LOCUS       DAZKAY010000246          388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000246 DAZKAY010000000
VERSION     DAZKAY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.262"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          128 a           59 c           49 g          152 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctattta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttagg
//
LOCUS       DAZKAY010000247          385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000247 DAZKAY010000000
VERSION     DAZKAY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.263"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>385
                     /locus_tag="ACG8WP_002921"
     CDS             <1..>385
                     /locus_tag="ACG8WP_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP9029125.1"
                     /translation="ELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGL
                     KIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALK
                     GITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          144 a           73 c           64 g          104 t
ORIGIN      
        1 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
       61 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
      121 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
      181 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
      241 cttcccaata tcctagggga caaaggatat attagccata aaatccatga aaagcttgcc
      301 cttaagggca ttactatatc cgttccgcca cgtaaaaaca tggataaatc agaaaaacta
      361 gaccacagct tacttggaaa acaaa
//
LOCUS       DAZKAY010000248          375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000248 DAZKAY010000000
VERSION     DAZKAY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.265"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8WP_002922"
     CDS             complement(<1..132)
                     /locus_tag="ACG8WP_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9029126.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAZKAY010000249          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000249 DAZKAY010000000
VERSION     DAZKAY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.266"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>371
                     /locus_tag="ACG8WP_002923"
     CDS             <1..>371
                     /locus_tag="ACG8WP_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9029127.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTP"
BASE COUNT          150 a           58 c           84 g           79 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct g
//
LOCUS       DAZKAY010000250          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000250 DAZKAY010000000
VERSION     DAZKAY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.267"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACG8WP_002924"
     CDS             <1..>367
                     /locus_tag="ACG8WP_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP9029128.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DAZKAY010000251          359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000251 DAZKAY010000000
VERSION     DAZKAY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.268"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..>359)
                     /locus_tag="ACG8WP_002925"
     CDS             complement(48..>359)
                     /locus_tag="ACG8WP_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029129.1"
                     /translation="NSKESYRRTAVSRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQ
                     TVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKL
                     T"
BASE COUNT          111 a           65 c           42 g          141 t
ORIGIN      
        1 tacaaagatt aaataaaaaa ataacaaaca aactcgcttg aaattcatta ggtgagtttg
       61 tttattattt tttctgcttc tcgattcatc agcgccatat ctgttttcgt aatggtttca
      121 ttaggaatat agtcagcaca ttttcttttc tctaacattc tatacccact attatgtatt
      181 cctaaggtat tcaattcctt aactgttgaa aacaaattcc aaacggtttg gtggcttcct
      241 tttccgctac ttccattata aataatatca taattttctt tcaaatactc atcagatttt
      301 ttaaaagcac tgtagtatgc tcttgaaaca gcagttcttc tataagattc tttagaatt
//
LOCUS       DAZKAY010000252          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000252 DAZKAY010000000
VERSION     DAZKAY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.269"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>352
                     /locus_tag="ACG8WP_002926"
     CDS             82..>352
                     /locus_tag="ACG8WP_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9029130.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRY"
BASE COUNT          125 a           55 c           73 g           99 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta ta
//
LOCUS       DAZKAY010000253          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000253 DAZKAY010000000
VERSION     DAZKAY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.271"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          124 a           54 c           59 g           96 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaa
//
LOCUS       DAZKAY010000254          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000254 DAZKAY010000000
VERSION     DAZKAY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.272"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8WP_002927"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8WP_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029131.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKAY010000255          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000255 DAZKAY010000000
VERSION     DAZKAY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.273"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          107 a           33 c           47 g          114 t
ORIGIN      
        1 aggactacct gtattagtcc tcttttttga ccagattttg accagtctat tttacattct
       61 aatatattca tatgagctaa ttttatgatt aaatggtaag tttttccttg tttgttttag
      121 tttaaactag tgtgtaattc aaaaatctat atggaaggat ctgtggggta aatcattata
      181 caaaagtaaa attttactta ttaacaggct gtagaaatat aaaaaagaac taaaatttaa
      241 ctaaattatg tgctattctt aaggaaagcc aagtgtatga gaaagtttct gagaatataa
      301 t
//
LOCUS       DAZKAY010000256          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000256 DAZKAY010000000
VERSION     DAZKAY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.274"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           51 c           43 g           96 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaa
//
LOCUS       DAZKAY010000257          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000257 DAZKAY010000000
VERSION     DAZKAY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.275"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8WP_002928"
     CDS             <1..>291
                     /locus_tag="ACG8WP_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029132.1"
                     /translation="AIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVK
                     KAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT           93 a           47 c           59 g           92 t
ORIGIN      
        1 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
       61 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      121 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      181 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      241 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt a
//
LOCUS       DAZKAY010000258          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000258 DAZKAY010000000
VERSION     DAZKAY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.276"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>281
                     /locus_tag="ACG8WP_002929"
     CDS             28..>281
                     /locus_tag="ACG8WP_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029133.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DAZKAY010000259          274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000259 DAZKAY010000000
VERSION     DAZKAY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.278"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>274)
                     /locus_tag="ACG8WP_002930"
     CDS             complement(<1..>274)
                     /locus_tag="ACG8WP_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFP9029134.1"
                     /translation="TLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFI
                     FLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILS"
BASE COUNT          111 a           43 c           36 g           84 t
ORIGIN      
        1 aaacttaaaa taatagaagt atatccaaac atagctccaa catttggact tgtatagaca
       61 ccaaataacc gatgttcaat aaaaccttgt cttgcaagta atcctggcgt tcctgttggg
      121 acatgataag aaatcaaaaa aataaattga tagatagata ttagcgcagc taaaaaaatt
      181 atgataatga aaacattatt aaaatacacc atttttttct ttttttcctc ttcactttgt
      241 gaaaagtctg caggaaaaat tactatcaaa gtaa
//
LOCUS       DAZKAY010000260          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000260 DAZKAY010000000
VERSION     DAZKAY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.279"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG8WP_002931"
     CDS             complement(34..>266)
                     /locus_tag="ACG8WP_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9029135.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZKAY010000261          261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000261 DAZKAY010000000
VERSION     DAZKAY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.280"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..>261
                     /locus_tag="ACG8WP_002932"
     CDS             101..>261
                     /locus_tag="ACG8WP_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029136.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          100 a           48 c           39 g           74 t
ORIGIN      
        1 tttattacgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
       61 aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata cccatcttac
      121 aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg ttgctaaaac
      181 tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt tcttcaagca
      241 aggaaaatca gccttagaat a
//
LOCUS       DAZKAY010000262          241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000262 DAZKAY010000000
VERSION     DAZKAY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.282"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8WP_002933"
     CDS             complement(<1..220)
                     /locus_tag="ACG8WP_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9029137.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAZKAY010000263          233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000263 DAZKAY010000000
VERSION     DAZKAY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.283"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZKAY010000264          227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000264 DAZKAY010000000
VERSION     DAZKAY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.284"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..227
                     /locus_tag="ACG8WP_002934"
                     /pseudo
     CDS             1..227
                     /locus_tag="ACG8WP_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAZKAY010000265          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000265 DAZKAY010000000
VERSION     DAZKAY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.denovo.285"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8WP_002935"
     CDS             <1..>224
                     /locus_tag="ACG8WP_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9029138.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKAY010000266        14891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.067, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000266 DAZKAY010000000
VERSION     DAZKAY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.067"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8WP_002936"
     CDS             260..1171
                     /locus_tag="ACG8WP_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9029139.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8WP_002937"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8WP_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9029140.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8WP_002938"
     CDS             2328..2612
                     /locus_tag="ACG8WP_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9029141.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="ACG8WP_002939"
     CDS             2978..3277
                     /locus_tag="ACG8WP_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029142.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="ACG8WP_002940"
     CDS             3320..4810
                     /locus_tag="ACG8WP_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9029143.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5158..5328
                     /locus_tag="ACG8WP_002941"
     CDS             5158..5328
                     /locus_tag="ACG8WP_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029144.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5342..5959
                     /locus_tag="ACG8WP_002942"
     CDS             5342..5959
                     /locus_tag="ACG8WP_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9029145.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5959..6636
                     /locus_tag="ACG8WP_002943"
                     /pseudo
     CDS             5959..6636
                     /locus_tag="ACG8WP_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            6947..7444
                     /locus_tag="ACG8WP_002944"
     CDS             6947..7444
                     /locus_tag="ACG8WP_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFP9029146.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            7445..7861
                     /locus_tag="ACG8WP_002945"
     CDS             7445..7861
                     /locus_tag="ACG8WP_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFP9029147.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            7881..9425
                     /locus_tag="ACG8WP_002946"
     CDS             7881..9425
                     /locus_tag="ACG8WP_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9029148.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            9568..9792
                     /locus_tag="ACG8WP_002947"
     CDS             9568..9792
                     /locus_tag="ACG8WP_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9029149.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            9807..10676
                     /locus_tag="ACG8WP_002948"
     CDS             9807..10676
                     /locus_tag="ACG8WP_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9029150.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            10657..11391
                     /locus_tag="ACG8WP_002949"
     CDS             10657..11391
                     /locus_tag="ACG8WP_002949"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9029151.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            11424..12332
                     /locus_tag="ACG8WP_002950"
     CDS             11424..12332
                     /locus_tag="ACG8WP_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9029152.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            12329..12871
                     /gene="sat4"
                     /locus_tag="ACG8WP_002951"
     CDS             12329..12871
                     /gene="sat4"
                     /locus_tag="ACG8WP_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFP9029153.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            12964..13758
                     /locus_tag="ACG8WP_002952"
                     /note="aph(3')-IIIa"
     CDS             12964..13758
                     /locus_tag="ACG8WP_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9029154.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            14034..14165
                     /locus_tag="ACG8WP_002953"
                     /pseudo
     CDS             14034..14165
                     /locus_tag="ACG8WP_002953"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            14241..14734
                     /locus_tag="ACG8WP_002954"
                     /pseudo
     CDS             14241..14734
                     /locus_tag="ACG8WP_002954"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         5324 a         2277 c         3088 g         4202 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa
     4921 ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa
     4981 gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt
     5041 ttaattttct atcttttata ggtcattaga gtatacctat ttgtcctaat atgattttag
     5101 cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag gagtaaaatg
     5161 aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt taatgagcaa
     5221 caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca atttgaaatt
     5281 gaaaaagtag atattgctga ttggttattg caaccgagag aattttagag gttggttgaa
     5341 aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag atcggcaatt
     5401 acaagcgtta cagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga
     5461 acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatattg ttattgttac
     5521 tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga atcaaattca
     5581 aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta ttgaagatga
     5641 aaatttaagg cgtttgatta atagccttgt cattgaattg tacaagtatc aagcagaatc
     5701 agaacgaaaa aaaattaagg aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg
     5761 caaattcaaa ggtcgtcagc ataaatttaa agaaaatgat ccacgtttaa agcatgcttt
     5821 cgatttgttt ttgaatggtt tatccgataa agaagttgaa gaacaaactg gaatcaatcg
     5881 ccgaacgttt agaaggtatc gagcaagata taacgtgaca gtcgatcaaa gaaaaaacaa
     5941 tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag ccaggcatta
     6001 gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac
     6061 ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca
     6121 gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt
     6181 cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgct
     6241 gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag agaaggtgaa
     6301 aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa aacgtataaa
     6361 agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa
     6421 ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg
     6481 ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa aggcgtacaa
     6541 ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta tcaacgccag
     6601 gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata gttttactaa
     6661 tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc acccattagg
     6721 tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta atctcagaag
     6781 atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa ctaaagtagt
     6841 tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata cttaccttat
     6901 ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt tgtttttgcg
     6961 agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt tcagtcataa
     7021 gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa gcgactgtga
     7081 aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca atacccttat
     7141 tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac gaaatgtttt
     7201 agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga actctgcgag
     7261 tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag cagataattt
     7321 taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca ttgtaaattc
     7381 cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa aggtggtgag
     7441 ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta ctgtcgatga
     7501 aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac tatcccttac
     7561 agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc gcattttgaa
     7621 aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag agatatttga
     7681 taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg tagagcttgc
     7741 cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag ataataagat
     7801 gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa gcgaggaata
     7861 aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga agtacagccg
     7921 caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct acagaaacag
     7981 aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt ataaagaaaa
     8041 ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt acgaacacta
     8101 aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac atcgacatgg
     8161 ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt caatacatta
     8221 gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc aacaccacgg
     8281 atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag gaaagccaga
     8341 gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga aaggtgcagg
     8401 tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta actattgtcc
     8461 ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt cagagtctag
     8521 tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga aaaccaagat
     8581 ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga gatgcccttc
     8641 tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat gaagggcatg
     8701 ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag ttatttttgc
     8761 aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc aagaacaaaa
     8821 gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat tgtggcgata
     8881 tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg cgatgcgtga
     8941 ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa gatgaacttt
     9001 atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat atgataaaga
     9061 cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa atttcagaga
     9121 ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac aagggtcaag
     9181 actatgacca cttagcagat gagattgatg agctgagaga caagcgacag atccttttag
     9241 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
     9301 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
     9361 gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc gaaatggaaa
     9421 tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt tttcttttgc
     9481 ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg gtttataata
     9541 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
     9601 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
     9661 ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
     9721 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga aataaaaata
     9781 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
     9841 aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt gcaagaggca
     9901 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
     9961 tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac aaccttgttg
    10021 tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt ataaacgggt
    10081 gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc aaagatacgg
    10141 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcta
    10201 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
    10261 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
    10321 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gcagaggata
    10381 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
    10441 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
    10501 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
    10561 ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat gaaattgtat
    10621 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
    10681 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
    10741 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
    10801 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
    10861 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
    10921 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
    10981 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
    11041 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
    11101 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
    11161 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
    11221 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
    11281 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
    11341 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
    11401 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
    11461 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
    11521 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
    11581 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
    11641 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
    11701 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
    11761 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
    11821 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
    11881 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
    11941 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
    12001 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
    12061 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
    12121 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
    12181 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
    12241 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
    12301 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
    12361 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
    12421 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
    12481 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
    12541 taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa
    12601 aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca
    12661 aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca
    12721 tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg
    12781 tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
    12841 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
    12901 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
    12961 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
    13021 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
    13081 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
    13141 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
    13201 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
    13261 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
    13321 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
    13381 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
    13441 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
    13501 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
    13561 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
    13621 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
    13681 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
    13741 ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt
    13801 ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat
    13861 cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag
    13921 aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc
    13981 ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca
    14041 ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca
    14101 aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat atccggacac
    14161 ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg
    14221 gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag
    14281 ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt
    14341 ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca
    14401 gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag
    14461 caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag
    14521 ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat gaacagaact
    14581 ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg tggatcggat
    14641 taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt tggaacaaat
    14701 attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt gtaaaaattg
    14761 gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag ccctttaaag
    14821 agggctttta tatattaatc acaaatcact tatcacaaat cacaagtgat taatcacaaa
    14881 tcacttgtga t
//
LOCUS       DAZKAY010000267        11252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.088, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000267 DAZKAY010000000
VERSION     DAZKAY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.088"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..946)
                     /locus_tag="ACG8WP_002955"
     CDS             complement(<1..946)
                     /locus_tag="ACG8WP_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9029155.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLK"
     gene            1215..2111
                     /locus_tag="ACG8WP_002956"
     CDS             1215..2111
                     /locus_tag="ACG8WP_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9029156.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2261..3202)
                     /locus_tag="ACG8WP_002957"
     CDS             complement(2261..3202)
                     /locus_tag="ACG8WP_002957"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9029157.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3328..4449
                     /locus_tag="ACG8WP_002958"
     CDS             3328..4449
                     /locus_tag="ACG8WP_002958"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9029158.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            4707..6209
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_002959"
     CDS             4707..6209
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9029159.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6276..6803
                     /locus_tag="ACG8WP_002960"
     CDS             6276..6803
                     /locus_tag="ACG8WP_002960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9029160.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7014..8075
                     /locus_tag="ACG8WP_002961"
     CDS             7014..8075
                     /locus_tag="ACG8WP_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9029161.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8078..8974
                     /gene="murQ"
                     /locus_tag="ACG8WP_002962"
     CDS             8078..8974
                     /gene="murQ"
                     /locus_tag="ACG8WP_002962"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9029162.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            8988..10970
                     /locus_tag="ACG8WP_002963"
     CDS             8988..10970
                     /locus_tag="ACG8WP_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9029163.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11035..>11252)
                     /locus_tag="ACG8WP_002964"
     CDS             complement(11035..>11252)
                     /locus_tag="ACG8WP_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9029164.1"
                     /translation="ETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGF
                     PFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3735 a         2027 c         2400 g         3090 t
ORIGIN      
        1 ccttcaaacg gaagaacaaa attcttgaag atgcatacac acccgaattg atagatgaga
       61 acaaagcaag gacaatcaca aaattgacga tatccgctgc aaatgggata ttcatttttt
      121 caaataccat cacaaaagga ctgacattcg tgcctgctaa agtattccaa ggaaaaagaa
      181 tcaaaagcaa aaacaaagga atgatgtaaa aggaaatgat tcttcccatg acacctttga
      241 ttgctttagg aatcgcacgt ttaggatcat ctgcttcgct taccgtgata gcaatcagtt
      301 cagaaccgcc gtaagaatag ataactacaa gcaaagaact gatgattccc ttcataccat
      361 gaggagtaaa cccaccatgg tcagtcaatt gctgaagggt agggaaaacg cctgtgccca
      421 acatttgctg accgacaaga taagtagcaa aaatgatcag taagataatg acactgattt
      481 tagcaaaagc cagccaatat tctgtttctg cgaataaccg taccgaataa agattgatga
      541 tcgtagttaa aacagccaac ataaaaacaa atacccaggc tggaatgtgc gggaaccaca
      601 actgcaaaaa ggaagcagct gcaacagctt cagcgatgat attgataatc cacatagacc
      661 agtagaccca atcagtaaaa tcagcagaat gagcacctag ataagggcgt atcaaaccag
      721 ataatccgtg atgcgtgtcg ccaccgctta aaacgatttt tcccagacct ttcatcacga
      781 tatataaaat cagcccacca atcaaaaaag caatcaatac agaagggccg gcgatcccaa
      841 tcgcttcgcc acttccttta aatagacctg caccgattgc tccacctaaa gctaacatgg
      901 tgatatgtct ggagctaagt tgttttttca attttttatc ttccataaat gacctctttc
      961 ttttaaattc acaatcaaaa tttatattaa gaataaatag caatatttaa caatgactaa
     1021 tgataacaaa ttttcagaca attacaacta ttcagaaaac ttttaggcga attttaatag
     1081 aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac aaatatagga
     1141 aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa
     1201 gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt
     1261 attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg
     1321 aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt tttttagatt
     1381 atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt
     1441 tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag gcacaagatg
     1501 aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa cttgggctga
     1561 aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg
     1621 atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc tataagcagg
     1681 aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga gggaatgaac
     1741 aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc
     1801 tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa tccatgtatt
     1861 ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt tatcgtcctg
     1921 aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt ctgcatacac
     1981 catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat tgggcaaaac
     2041 gatttatcac taacgattcc actatttatg agcaatacga atatgacctc tcctctgttg
     2101 aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac aagcacaatt
     2161 cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt aaaaaaagag
     2221 aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg ccaaggattc
     2281 cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt
     2341 tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt
     2401 tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag
     2461 aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt gctgcgatag
     2521 aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc gcgcctagtt
     2581 cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt acacaagctt
     2641 ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt gcttcttgaa
     2701 agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga
     2761 tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct gtccacatcg
     2821 tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct tcatgaccta
     2881 atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg atcgaacaaa
     2941 acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg tgttcgttgc
     3001 tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct ttaccgttaa
     3061 agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca
     3121 agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc attgctccgg
     3181 ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt
     3241 atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct
     3301 tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa
     3361 ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa
     3421 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
     3481 tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga
     3541 aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca
     3601 caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc
     3661 agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt
     3721 gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt
     3781 gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt
     3841 gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat
     3901 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat
     3961 acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc
     4021 tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat tggtggagcc
     4081 ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga
     4141 ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag
     4201 caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa
     4261 ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc
     4321 ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg
     4381 caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc
     4441 ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag
     4501 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
     4561 ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat
     4621 gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact
     4681 ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg
     4741 gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat
     4801 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac
     4861 aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc
     4921 aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg
     4981 agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga
     5041 gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg
     5101 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta
     5161 gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg
     5221 accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg
     5281 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
     5341 aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg
     5401 aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca
     5461 tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga
     5521 tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac
     5581 tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata
     5641 agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa
     5701 ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa
     5761 agtcatccat tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat
     5821 accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa
     5881 cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc
     5941 gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc
     6001 aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg
     6061 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga
     6121 acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta
     6181 ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg
     6241 ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag
     6301 aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa
     6361 aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca
     6421 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc
     6481 gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg
     6541 atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat
     6601 tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat
     6661 gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga
     6721 tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata
     6781 cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg
     6841 cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac
     6901 tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta
     6961 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     7021 gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa
     7081 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt
     7141 cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat
     7201 tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa
     7261 cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc
     7321 agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta
     7381 aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata
     7441 attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat
     7501 ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag
     7561 gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag
     7621 gcgctctaga tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt
     7681 cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     7741 cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag
     7801 acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc
     7861 cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt
     7921 tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga
     7981 atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta
     8041 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     8101 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca
     8161 agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact
     8221 gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct
     8281 gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt
     8341 accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc
     8401 aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat
     8461 caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     8521 atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc
     8581 ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg
     8641 tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt
     8701 gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt
     8761 cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc
     8821 gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa
     8881 gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc
     8941 tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg
     9001 aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg
     9061 tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg
     9121 taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc
     9181 ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg
     9241 caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag
     9301 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
     9361 aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa
     9421 tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact
     9481 tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca
     9541 tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag
     9601 tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga
     9661 tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag
     9721 gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc
     9781 ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca
     9841 tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg
     9901 tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca
     9961 cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc
    10021 aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag
    10081 gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag
    10141 aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg
    10201 gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg
    10261 gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg
    10321 ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag
    10381 cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    10441 aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc
    10501 aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt
    10561 tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag
    10621 aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa
    10681 cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa
    10741 tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag
    10801 tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag
    10861 aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    10921 aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa
    10981 acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt
    11041 attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc
    11101 ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata
    11161 ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc
    11221 ttcatgttcc agatattcac gcatcgtttc gg
//
LOCUS       DAZKAY010000268        11252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.089, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000268 DAZKAY010000000
VERSION     DAZKAY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.089"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..946)
                     /locus_tag="ACG8WP_002965"
     CDS             complement(<1..946)
                     /locus_tag="ACG8WP_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9029165.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLK"
     gene            1215..2111
                     /locus_tag="ACG8WP_002966"
     CDS             1215..2111
                     /locus_tag="ACG8WP_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9029166.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2261..3202)
                     /locus_tag="ACG8WP_002967"
     CDS             complement(2261..3202)
                     /locus_tag="ACG8WP_002967"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9029167.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3328..4449
                     /locus_tag="ACG8WP_002968"
     CDS             3328..4449
                     /locus_tag="ACG8WP_002968"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9029168.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            4707..6209
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_002969"
     CDS             4707..6209
                     /gene="eat(A)"
                     /locus_tag="ACG8WP_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9029169.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLYISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6276..6803
                     /locus_tag="ACG8WP_002970"
     CDS             6276..6803
                     /locus_tag="ACG8WP_002970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9029170.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7014..8075
                     /locus_tag="ACG8WP_002971"
     CDS             7014..8075
                     /locus_tag="ACG8WP_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9029171.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8078..8974
                     /gene="murQ"
                     /locus_tag="ACG8WP_002972"
     CDS             8078..8974
                     /gene="murQ"
                     /locus_tag="ACG8WP_002972"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9029172.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            8988..10970
                     /locus_tag="ACG8WP_002973"
     CDS             8988..10970
                     /locus_tag="ACG8WP_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9029173.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11035..>11252)
                     /locus_tag="ACG8WP_002974"
     CDS             complement(11035..>11252)
                     /locus_tag="ACG8WP_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9029174.1"
                     /translation="ETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGF
                     PFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3735 a         2027 c         2399 g         3091 t
ORIGIN      
        1 ccttcaaacg gaagaacaaa attcttgaag atgcatacac acccgaattg atagatgaga
       61 acaaagcaag gacaatcaca aaattgacga tatccgctgc aaatgggata ttcatttttt
      121 caaataccat cacaaaagga ctgacattcg tgcctgctaa agtattccaa ggaaaaagaa
      181 tcaaaagcaa aaacaaagga atgatgtaaa aggaaatgat tcttcccatg acacctttga
      241 ttgctttagg aatcgcacgt ttaggatcat ctgcttcgct taccgtgata gcaatcagtt
      301 cagaaccgcc gtaagaatag ataactacaa gcaaagaact gatgattccc ttcataccat
      361 gaggagtaaa cccaccatgg tcagtcaatt gctgaagggt agggaaaacg cctgtgccca
      421 acatttgctg accgacaaga taagtagcaa aaatgatcag taagataatg acactgattt
      481 tagcaaaagc cagccaatat tctgtttctg cgaataaccg taccgaataa agattgatga
      541 tcgtagttaa aacagccaac ataaaaacaa atacccaggc tggaatgtgc gggaaccaca
      601 actgcaaaaa ggaagcagct gcaacagctt cagcgatgat attgataatc cacatagacc
      661 agtagaccca atcagtaaaa tcagcagaat gagcacctag ataagggcgt atcaaaccag
      721 ataatccgtg atgcgtgtcg ccaccgctta aaacgatttt tcccagacct ttcatcacga
      781 tatataaaat cagcccacca atcaaaaaag caatcaatac agaagggccg gcgatcccaa
      841 tcgcttcgcc acttccttta aatagacctg caccgattgc tccacctaaa gctaacatgg
      901 tgatatgtct ggagctaagt tgttttttca attttttatc ttccataaat gacctctttc
      961 ttttaaattc acaatcaaaa tttatattaa gaataaatag caatatttaa caatgactaa
     1021 tgataacaaa ttttcagaca attacaacta ttcagaaaac ttttaggcga attttaatag
     1081 aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac aaatatagga
     1141 aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa
     1201 gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt
     1261 attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg
     1321 aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt tttttagatt
     1381 atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt
     1441 tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag gcacaagatg
     1501 aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa cttgggctga
     1561 aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg
     1621 atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc tataagcagg
     1681 aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga gggaatgaac
     1741 aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc
     1801 tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa tccatgtatt
     1861 ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt tatcgtcctg
     1921 aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt ctgcatacac
     1981 catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat tgggcaaaac
     2041 gatttatcac taacgattcc actatttatg agcaatacga atatgacctc tcctctgttg
     2101 aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac aagcacaatt
     2161 cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt aaaaaaagag
     2221 aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg ccaaggattc
     2281 cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt
     2341 tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt
     2401 tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag
     2461 aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt gctgcgatag
     2521 aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc gcgcctagtt
     2581 cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt acacaagctt
     2641 ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt gcttcttgaa
     2701 agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga
     2761 tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct gtccacatcg
     2821 tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct tcatgaccta
     2881 atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg atcgaacaaa
     2941 acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg tgttcgttgc
     3001 tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct ttaccgttaa
     3061 agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca
     3121 agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc attgctccgg
     3181 ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt
     3241 atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct
     3301 tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa
     3361 ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa
     3421 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
     3481 tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga
     3541 aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca
     3601 caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc
     3661 agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt
     3721 gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt
     3781 gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt
     3841 gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat
     3901 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat
     3961 acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc
     4021 tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat tggtggagcc
     4081 ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga
     4141 ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag
     4201 caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa
     4261 ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc
     4321 ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg
     4381 caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc
     4441 ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag
     4501 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
     4561 ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat
     4621 gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact
     4681 ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg
     4741 gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat
     4801 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac
     4861 aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc
     4921 aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg
     4981 agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga
     5041 gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg
     5101 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttgtat atctctggta
     5161 gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg
     5221 accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg
     5281 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
     5341 aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg
     5401 aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca
     5461 tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga
     5521 tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac
     5581 tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata
     5641 agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa
     5701 ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa
     5761 agtcatccat tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat
     5821 accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa
     5881 cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc
     5941 gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc
     6001 aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg
     6061 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga
     6121 acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta
     6181 ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg
     6241 ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag
     6301 aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa
     6361 aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca
     6421 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc
     6481 gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg
     6541 atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat
     6601 tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat
     6661 gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga
     6721 tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata
     6781 cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg
     6841 cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac
     6901 tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta
     6961 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     7021 gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa
     7081 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt
     7141 cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat
     7201 tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa
     7261 cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc
     7321 agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta
     7381 aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata
     7441 attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat
     7501 ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag
     7561 gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag
     7621 gcgctctaga tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt
     7681 cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     7741 cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag
     7801 acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc
     7861 cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt
     7921 tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga
     7981 atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta
     8041 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     8101 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca
     8161 agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact
     8221 gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct
     8281 gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt
     8341 accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc
     8401 aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat
     8461 caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     8521 atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc
     8581 ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg
     8641 tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt
     8701 gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt
     8761 cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc
     8821 gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa
     8881 gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc
     8941 tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg
     9001 aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg
     9061 tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg
     9121 taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc
     9181 ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg
     9241 caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag
     9301 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
     9361 aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa
     9421 tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact
     9481 tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca
     9541 tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag
     9601 tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga
     9661 tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag
     9721 gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc
     9781 ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca
     9841 tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg
     9901 tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca
     9961 cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc
    10021 aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag
    10081 gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag
    10141 aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg
    10201 gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg
    10261 gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg
    10321 ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag
    10381 cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    10441 aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc
    10501 aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt
    10561 tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag
    10621 aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa
    10681 cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa
    10741 tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag
    10801 tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag
    10861 aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    10921 aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa
    10981 acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt
    11041 attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc
    11101 ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata
    11161 ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc
    11221 ttcatgttcc agatattcac gcatcgtttc gg
//
LOCUS       DAZKAY010000269         7958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.114, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000269 DAZKAY010000000
VERSION     DAZKAY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.114"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8WP_002975"
     CDS             128..1564
                     /locus_tag="ACG8WP_002975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9029175.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8WP_002976"
     CDS             1753..4200
                     /locus_tag="ACG8WP_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9029176.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8WP_002977"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8WP_002977"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9029177.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8WP_002978"
     CDS             5487..5693
                     /locus_tag="ACG8WP_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029178.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8WP_002979"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8WP_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9029179.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8WP_002980"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8WP_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9029180.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZKAY010000270         5641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000270 DAZKAY010000000
VERSION     DAZKAY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.134"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..804
                     /locus_tag="ACG8WP_002981"
     CDS             112..804
                     /locus_tag="ACG8WP_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9029181.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1131..1628
                     /gene="dfrG"
                     /locus_tag="ACG8WP_002982"
     CDS             1131..1628
                     /gene="dfrG"
                     /locus_tag="ACG8WP_002982"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9029182.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1700..3652)
                     /locus_tag="ACG8WP_002983"
     CDS             complement(1700..3652)
                     /locus_tag="ACG8WP_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9029183.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3659..3895)
                     /locus_tag="ACG8WP_002984"
     CDS             complement(3659..3895)
                     /locus_tag="ACG8WP_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029184.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4297..4587
                     /locus_tag="ACG8WP_002985"
     CDS             4297..4587
                     /locus_tag="ACG8WP_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9029185.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4859..5208)
                     /locus_tag="ACG8WP_002986"
                     /pseudo
     CDS             complement(4859..5208)
                     /locus_tag="ACG8WP_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5301..5603
                     /locus_tag="ACG8WP_002987"
                     /pseudo
     CDS             5301..5603
                     /locus_tag="ACG8WP_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1915 a          905 c          980 g         1841 t
ORIGIN      
        1 atttgatagt atttatatta aaattacata tgtaatttaa aaacgcttga cattgtgttt
       61 gagatcactt acttttagca tgtaaaagta ttggattaag gagctatgca aatgaaaaag
      121 aaatttatta ctggtgggat tgtcgttgca tttctatgtc taattggcta cacaggcacg
      181 cggtacgcaa atgaaattat tttgtggggt ggagagacag atattgatac aatcaatgag
      241 aatctgacac agcttgatca tacattgaca gataaagagc aagcgattac agatcttacc
      301 gctcgactag cacaaaaaga acagacattg caagattcaa aggcagatgt ccaagaatac
      361 caaacaaaag taaatgaatt agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac
      421 ttagaaagtc aattagtcag caaggatgca caactacaaa gcaaacaaaa tgaaatcaat
      481 gtcaaactag atgagatcaa ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg
      541 acagcaagac tacaagaagc acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt
      601 ttgaatcaac aaatcgcaac actgacacaa gaaaaaacaa atgtagaaac tcagttaaca
      661 gatacacaaa agaaattaga cgaagcaact gctgaaaact caagcttgaa acaatatatt
      721 caaaagttag aaaaagcaaa agatgaagtg gaagatacag ctgataaatc tcagcaaatt
      781 gttgaagaac atttgcgtaa ataatctttc ttaacaagta aacctaaatt agtaaagaag
      841 caacagtaat ctatactaag tagttcagtt ttggagtaca aaatgacaaa atgtacttca
      901 aaatggacta cttttttcat tttaacttac ataccaaatt acatacaaaa tgatttataa
      961 aatgcaattg aaaatgataa ttgaatgaaa atttgtgatt ttgtggtatt atgataatat
     1021 aacttataga aaaaattgct tattcaacta acgggcagga taatgagaaa aggttttttg
     1081 atagaaataa gaaattagat ggtaaattag ttaaaaaggc tgtgttataa atgaaagttt
     1141 ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac attccttgga
     1201 ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg ataatattag
     1261 gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat attattctga
     1321 cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata gaagatgttt
     1381 ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag atttataatt
     1441 tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa ttcgaaggag
     1501 atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc caaaaaggga
     1561 taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga aaaaacttat
     1621 tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa gagcttggga
     1681 ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca aattgtttat
     1741 ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat ttaaaagggc
     1801 gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa ttgagcatac ttcggattga
     1861 agttagcgta aatatagctc ttcttatcac cacgagctgt tgttaatggt ctttcaagag
     1921 atgatatata acgtcttatc tgttgaataa agctattggt cgaatggtca ttaacattta
     1981 atagcatctt tgcgatttcg tttggttcca gagcagataa atccgttcta caatcaactg
     2041 aataaaatcc tttatcctta gtggctaatg gatgtttgat agggttatcg gcatatttgc
     2101 gatatctttc tccatcttta ctaatagcct cttcgtggaa ttgatgactt gttagtaatt
     2161 cagttagata atgagatgcc aaagtccgta gaaactttgt tttgaatcct ctgatatctc
     2221 cccaatcgag cagttctgct tttgcttgtc cgaattcttt taagcattgt tttctgcttt
     2281 tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct caaccttaat tctttcgtga
     2341 atactctata aaaggcgttt ctaatagaga agtcatcatc actaatcatt cgccattcgt
     2401 cggcattaac catttgttta attaaccaat agtgagcagt tgtagtataa ggagcatcaa
     2461 catttaaacc atcgacaaat ctactcctat tagtaatttt ccctaattcg tgaagataag
     2521 cattcctatt ttcgctatcg ttagcggatg gttcttgtgg ataataagac caatctaacc
     2581 tatcattttt acgactaaaa gtattgagat ggtcttcttt gaattttctt gtatcttcat
     2641 ttaattcatc cattgaaata ttccaatcat aggcaacatc agaacggaac acatatcttg
     2701 ataagacctc agcagataca actatatacg tttgcatatt aaggtcttca aaccccgtat
     2761 atttctttga accttgtcct ttcttacgga cgttatttag gtagtagtgc atcttgtccg
     2821 tattaagcca catctcgtta aatttctttg tttgaagggg ttgtgtctca tatcgttcta
     2881 aaaactccaa acaacgccta tacagccact ccagcttatg atagtaggtt ccaactccaa
     2941 ttcctaaaat atcacaagta cgactgactg aaacccttcc aacaaccatc ttagcgaaca
     3001 ttggaagaat ggtgttcttt tgttggtgat aagtagtggt ttcttctctt tttggtagaa
     3061 cattagtgaa cttcttacac gccttacatt gatagcgttg agatttgccc ctacttttcc
     3121 cccttttgta aaattgcttc ggctcattga atggggttga ttcttcttcc gagcagcctt
     3181 ctttatgaaa ttgataatct ggctcaacat cttggatgct attgatttct ataaggcgtt
     3241 taatttcttc aacaacagac caattagata aaggggttac tgtacagttt tgcgatacac
     3301 ctctgccaat aggattgtca ttgcagtaca tacctttatg acctttatct ttggaactac
     3361 cagtcatttt atatcgactt ggtttccctt ttacatcttt gaatttgtgc tgttctttcc
     3421 caaaattaca acaaaacgga ttgatgcagt aattatattg aatgggatat tcagaaccgt
     3481 tccatttaaa agacgttggc aagaacaaca ttttgttata ccttgttgca aatcgttttg
     3541 gagttaataa atcatattgt ttagccctat cttttaattc ttcgtttgaa atcggaattt
     3601 taacatctac aatttcatct tccttcgtgg ctaatctttt aagcttggtc attgtctatc
     3661 aacccactct atctttgata cccatttttt caacaatccg cttattactt cgcttgttgg
     3721 caatccattc aataaacttc ttatcgtcta aaatgttcaa taagaattca tccactgctt
     3781 cgctttcagt acgctctgca tattcagcgt attgcttaac tatcaccctt acttgctctg
     3841 atatttccca atccacagac ttagcgttct tattttttgg ctcaagaaat tgcataggaa
     3901 aatgcacccc tttttgctta gtaaattgta accattttaa cacaccaatt tgcattccta
     3961 caataattat ttcacgttcg catttttata tacattttaa cttacatttt gtggtgtaaa
     4021 ttaacaacaa acgttgattt aacaataaaa aacgagtatc aaattcagtt gcaatttgat
     4081 actcatttag gtttacattt tcactttttg tactccaaaa ctgaactact taaatctata
     4141 ctgctgcctc ttttttttag tacatgtcct tcatcttcgc aggaagtgcc aatatgattt
     4201 taagaaaggt aatagacaaa aagtcaaaaa gataaaacag agtttcttct ttacaaaggg
     4261 aatgtatgtt cgtataatgg tattgaggtg aaggttatgg agaaaaaata tggaacagta
     4321 gataagatcc gtactttgca agttagtgcc agaccgttga tccgtttcag tttaaatggg
     4381 gtcaattgtt tgattgcttc ccatagtttg aacttcctag ctgaagtaga cgaagggatg
     4441 aagttagtcg ttacaggtta ttataataat agaaagcaat ttgttgtgag atcctatcat
     4501 gtgttaggga aacccaaaat tgtggtagaa tatgagaaaa gtctataccc aagaaaaaag
     4561 gtccgacaag atgaaagaat aaactagaaa acaagcggct gagacaaaaa tgtcctagcc
     4621 gcttgttttg gaaagatgga tgcagacgat gggatttgaa cccacacgag catacgctca
     4681 caagaacctc aatctatata taactacatg tatttaagta tttgtgatga atcactaaac
     4741 tttccaaact catttgaggg atattgctta aagattgaac agatcaattc gaagccctat
     4801 tagtattcgc tcaatggggg gatcagaggc cataaaaaga gactagtcat tactagtctc
     4861 aggtagtgga attgtaggat tttaaacgtt cctggagagt accggtatgt aactcgaaga
     4921 ggtgattatc atagtcgtaa aagtaaattg aagaagcctc gcctttcaca cgagaacgtc
     4981 ctggttcgac agttaaacct aacatttgga tttttgagac aaacgaatct atatcttgtt
     5041 cgtcaatttt aaaagcaata tgattgtatg tctttggcag tttattcgac gaattctgca
     5101 ttactgcaat ccataggtcc ttaactaaga aaaatttttc tggatataat gaaaattttt
     5161 ttgttgacta tcgtagattt cttttgcatc aaataaggta tttaacaatt aagtatttaa
     5221 aagataactt ttaagttatc tttaagtaat gcttaaagct atgttatact ctattaggaa
     5281 atataagaag gagtataaaa atgaacaaac ataatagaaa aataacgaat aacaaagaac
     5341 tgatcttgtt actaataggg atttggggta tctttttact ctttaatatg tatatgccga
     5401 ctatgagagc ggatgacgta gtttatgcaa gtagattaga tgaacttggc tatttgggtg
     5461 cttcgataga acattataag acatggagtt caagaattat tatagaacta ttcttaatgt
     5521 tcttttctaa acactttatg ctatggaaat tattaaattc tacgattatg ttaggaattg
     5581 tagtattact ttgcaagtat gttgagagta taaaatattt tgtgtaaatg aaaaaatcca
     5641 t
//
LOCUS       DAZKAY010000271         4800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.146, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000271 DAZKAY010000000
VERSION     DAZKAY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.146"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..784
                     /gene="vanR-A"
                     /locus_tag="ACG8WP_002988"
     CDS             89..784
                     /gene="vanR-A"
                     /locus_tag="ACG8WP_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9029186.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            762..1916
                     /locus_tag="ACG8WP_002989"
     CDS             762..1916
                     /locus_tag="ACG8WP_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9029187.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2131..3099
                     /locus_tag="ACG8WP_002990"
     CDS             2131..3099
                     /locus_tag="ACG8WP_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9029188.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3092..4123
                     /gene="vanA"
                     /locus_tag="ACG8WP_002991"
     CDS             3092..4123
                     /gene="vanA"
                     /locus_tag="ACG8WP_002991"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9029189.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4129..4737
                     /locus_tag="ACG8WP_002992"
     CDS             4129..4737
                     /locus_tag="ACG8WP_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9029190.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1279 t
ORIGIN      
        1 ggccttttat cttttacaca attatatgga ctttatccta taatctgata aattattaag
       61 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      121 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      181 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      241 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      301 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      361 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      421 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      481 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      541 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      601 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      661 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      721 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      781 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
      841 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
      901 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
      961 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1021 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1081 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1141 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1201 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1261 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1321 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1381 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1441 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1501 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1561 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1621 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1681 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1741 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1801 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     1861 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     1921 tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag gaaattaaca
     1981 atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta agaacgagcc
     2041 gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg
     2101 tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga
     2161 tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca
     2221 acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt
     2281 atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc
     2341 ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct
     2401 aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat
     2461 actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa
     2521 aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt
     2581 gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt
     2641 aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag
     2701 ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat
     2761 attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg
     2821 cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc
     2881 ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa
     2941 aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca
     3001 ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa
     3061 aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt
     3121 tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa
     3181 cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa
     3241 aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc
     3301 gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca
     3361 tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg
     3421 tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg
     3481 tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt
     3541 ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt
     3601 taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt
     3661 ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt
     3721 ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga
     3781 ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga
     3841 aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg
     3901 acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg
     3961 tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc
     4021 cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc
     4081 cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga
     4141 tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg
     4201 gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac
     4261 gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg
     4321 cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca
     4381 cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg
     4441 atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt
     4501 acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg
     4561 gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga
     4621 cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac
     4681 tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt taaataaact
     4741 tttaaccgtt gcacggacaa acggttctgt tgcaaagttt taaataaaga ataaaatccc
//
LOCUS       DAZKAY010000272         4324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.157, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000272 DAZKAY010000000
VERSION     DAZKAY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.157"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..1752
                     /gene="msr(C)"
                     /locus_tag="ACG8WP_002993"
     CDS             274..1752
                     /gene="msr(C)"
                     /locus_tag="ACG8WP_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9029191.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1799..2245
                     /locus_tag="ACG8WP_002994"
     CDS             1799..2245
                     /locus_tag="ACG8WP_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9029192.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2444..2959)
                     /locus_tag="ACG8WP_002995"
     CDS             complement(2444..2959)
                     /locus_tag="ACG8WP_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9029193.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3076..4278
                     /locus_tag="ACG8WP_002996"
     CDS             3076..4278
                     /locus_tag="ACG8WP_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9029194.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1298 a          728 c          934 g         1364 t
ORIGIN      
        1 atgactgcat cgatgaaatt acgtttcgaa cttttgaata acaactaaat aaattcaaaa
       61 gacatgtata aacaacaatt tcatggatgg agtatgtcca ttttttcgaa cgacagtaga
      121 ataggtgaaa gctatgacag atttggtgat ttagtcatag ttatttcgtt cagagtagga
      181 aaagatgtga tgcagttttt ttgcaaacag atttttctgc tcttttttag tatcgataat
      241 tgttgtttta catgaaatag gagtgtaaaa catatggaaa atttagcagt aaatataaca
      301 aatctacaag tcagctttgg caaccagcta gaactatcta ttgattctct tcgtgtctat
      361 cagcaagatc gtatagggat cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa
      421 ctaatagccg gtgaactttt tcccgatcat gggaaaatcc aaacagagat caccttcaac
      481 tacctgcctc aattaaccta tctcgctgaa gcaaaggacc taaatttgga attagccagt
      541 catttccagt taagactgga agaaacttca gagcggaaat ggagcggagg ggaagaacga
      601 aagatcgagt tgatacgtct tctttcttct tatgaacaag ggatgcttct agacgagcca
      661 acaacccatc tagatagaaa aagtattgat cgactgattg aagagcttcg ttattattat
      721 ggtacgctgg tttttgttag tcatgatcgc tattttctag atgagctagc atcgaaaatc
      781 tgggaagtaa aagacggaga aatccgagag ttttcgggga attatagtgc ctatctcact
      841 caaaaggaat tggagaaaaa gactcagcta cgagaagcag agtcgatcat gaaagagaaa
      901 aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg
      961 tccagtaaaa agaaaaagca acaaatcaga ccggatcggt tgtcttcctc taaacaaaaa
     1021 gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat tagagagaag actccaaaaa
     1081 ataggagaaa caaccaagcc gcagcagatg aaacaaatcc gttttccagt accaaaatct
     1141 cttgaactcc acagccgcta tcccatcatg ggacaaaatg tccaattgga aagaagtggg
     1201 agaacattac tggtaaatgg tgattttcag ttttctttag gtaaaaaaat cgcgattgtc
     1261 ggtgaaaatg gttcaggtaa gacaacctta ttagaacata tccgcaaaca aggagaagga
     1321 atccttctct ctccgaaagt aagctttcaa gtatatcagc aaaagggtta tcaaatgaca
     1381 tctgaagaat ccatcattcg ttttgtcatg agacaaacag agttttcaga atcgcttgtc
     1441 cgtagtttgc tgaatcactt agggtttgct caggaaactt tgacgaaacc gttatgtaca
     1501 ttaagtgggg gagaagcgac ccgtctgacg attgctttgc tttttactaa gccaagtaat
     1561 gtgttgctgt tagatgaacc gactaatttt attgatatgg caacgatcga agctttagag
     1621 aagctaatgc aaatatatcc gggaacgatt ttgtttactt ctcatgattc ttactttgtc
     1681 gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa agtacttacg
     1741 agaaattttt aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt
     1801 gcctacaaat aaaaaagaag gaataatttt tacaacaatg atgtgttttt tgatggtctt
     1861 ggggatgagc gcgtataatt tatggctgca tcaagatctt catttatcaa gtttgttgat
     1921 tggtctagtg cctggattcg tggtggcttt cattttggat gtcttcatca tcggtgtgct
     1981 ggcgaaaaaa attgccttta aactaccagt aaatccagaa agcaaggtcc aaatgatctt
     2041 aactatttca tgcttgatgg ttttaggaat ggtgacatgt atgtctttat tcggtgtgat
     2101 catggaacaa ggaatacctg atcaattatt ctctgcttat atgaatgctt ggaaaatgaa
     2161 tgtgattgcc gctttaccgt tgcagttgat catcgttggc ccgctttcaa gaagtgtgct
     2221 aaaagcaatt caaagtaatg aatgatggaa cactgttatt tgaaaaaagg ccttggcatg
     2281 ctttttgtca tagtctcttg aatgcagttc tagaaaaata gtgatccgaa cgataggttt
     2341 tggcctgtcg ttcggatcgc tttttctttt ccaactgtat tgctctttta ttgaagataa
     2401 tttgctcttc aatagatatt tctagaaagg ttctgtttat cccttactca aaaaactgct
     2461 gcatttgttc tcttacttgc tgacaccatt ggagttcctg ctgacaaagc ataattctgc
     2521 gctggatcac aacataacgt ccaaattctt gtttcttgtc tagcgagagg tcattcaatt
     2581 ttttttgatt tttttgcatg attttgttca gctgttgttc tctagtgaat agcagtccag
     2641 tagctgttgt tttatcgagc gtcgaaaaag catagatttt tgctagaaat tcatcttttt
     2701 gcgtaggagc gggagtttcc tctttgatcc attcctctac aagagcacgt ccttcatcag
     2761 tcaagtggta gatctttttt tgagagtgct cgtctttgtc caaaacggtc acataaccct
     2821 gctggtctaa cttggcaagc gttgtataga tctgactgtg atgggcttcc caaaaaattg
     2881 tgatcagctg cttcaattca tacccagaac gtggtttgga taaaagcacg cataataata
     2941 tataggctaa agtattcata aaaatcctac ctctcgtctt attttcttta ttatatcaca
     3001 gtaaagtttc cacgttttac atgtgcatat ttacatgtaa aataaatcat gtatgatgct
     3061 aatagagaga aagggatgga aatattggag aatcaaatag atatacaaga acaaacaaat
     3121 gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag ctacctgtat gatcgctttt
     3181 atgggagtgg gtctagtaga cccaatctta aaaacgattg ctgcacagtt aaacgctacg
     3241 ccagctgaaa cgaccctgtt atttactagt tacatgttag tcactggtgt ggtgatgcta
     3301 tttacaggtt tcttatctag tcggatcggt ttgaaaaaaa cattgatgac aggattgttt
     3361 atcatcgttg tattcgctgg tcttggcgga ttatctggga atatcggaat gctgattggg
     3421 ttacgtgccg gctggggtgt cgggaatgct ttatttgtct ctactgcttt agcaacgatc
     3481 gtcagtattt tagttggtaa cacagaaaaa gcaatcatga tgtatgaagc agcacttggc
     3541 tgcggaatgg ctgttggccc tctagtcggt ggggtcttgg gcagtggttc atggcgttat
     3601 ccattttttg gggtagcatt tttgatgttt tgcggtttta tagccctagc ggtactttta
     3661 cctgaaactg agaaaccaaa gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc
     3721 aatcataaac ttcgttcagt tggactaatc gcattgttat ataatttcgg attctttact
     3781 ttacttgcgt atgcgccatt tttattgatt ggctattctg aattagaagt cggttttgta
     3841 ttttttggct ggggtgtgct tctggctatt gcttcgattt tcgttgcacc ggctcttgag
     3901 cggaagttta cgacttatcg gacaatttta gcagcatttg tcttattcat tatttgtttg
     3961 ctgttattag gagctggaac ttctatgcca gtccttgtac cgatcagcat tgtgttagca
     4021 ggatttttcc aaggaattat caatacactg ttgacgacga ttgctatgga aataccaggt
     4081 ctgcaacgaa acgttgcttc gtccagctat agttttgtcc gctttttcgg tggagcttta
     4141 gcaccattta ttgcaggtgt gattggtgaa catattggtg aaagctatgc gttttatttt
     4201 gcaggtgcca ttgttcttat cagtgtatgt tgtatcattt atcaccgtaa ttattttgct
     4261 acaaatgaag aagaataaga aatcagaggt tgtgaaaaag ctgtcctgca tcaagtcata
     4321 agaa
//
LOCUS       DAZKAY010000273         2772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.175, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000273 DAZKAY010000000
VERSION     DAZKAY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.175"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8WP_002997"
     CDS             111..194
                     /locus_tag="ACG8WP_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9029195.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8WP_002998"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8WP_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9029196.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG8WP_002999"
     CDS             complement(1319..2182)
                     /locus_tag="ACG8WP_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9029197.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG8WP_003000"
     CDS             complement(2184..2456)
                     /locus_tag="ACG8WP_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9029198.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG8WP_003001"
     CDS             complement(2473..2682)
                     /locus_tag="ACG8WP_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9029199.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZKAY010000274         2305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000274 DAZKAY010000000
VERSION     DAZKAY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.180"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1253
                     /gene="mef(H)"
                     /locus_tag="ACG8WP_003002"
                     /gene_synonym="mefH"
     CDS             15..1253
                     /gene="mef(H)"
                     /locus_tag="ACG8WP_003002"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFP9029200.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1480..1899
                     /locus_tag="ACG8WP_003003"
     CDS             1480..1899
                     /locus_tag="ACG8WP_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9029201.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1904..2143
                     /locus_tag="ACG8WP_003004"
     CDS             1904..2143
                     /locus_tag="ACG8WP_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9029202.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          723 a          318 c          434 g          830 t
ORIGIN      
        1 aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg
       61 ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact
      121 atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt
      181 tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa
      241 acattatggt catactagat tttgtaacag caatcataat tttagtattt tatttgtcat
      301 tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt
      361 ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac
      421 ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag
      481 ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta
      541 tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac
      601 ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt
      661 tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg
      721 ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc
      781 cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta
      841 ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta
      901 tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag
      961 tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg
     1021 ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa
     1081 tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta
     1141 ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt
     1201 caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg
     1261 ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa
     1321 ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt
     1381 tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa acggtattcc
     1441 gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag
     1501 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
     1561 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
     1621 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
     1681 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
     1741 ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
     1801 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
     1861 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gaaatgagta aagattatgg
     1921 ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga
     1981 gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc
     2041 tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga
     2101 catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa
     2161 tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc
     2221 attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa
     2281 tgtatagtgt ttatgcggat aattg
//
LOCUS       DAZKAY010000275         1229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000275 DAZKAY010000000
VERSION     DAZKAY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.211"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACG8WP_003005"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACG8WP_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFP9029203.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          529 a           95 c          201 g          404 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttacca
//
LOCUS       DAZKAY010000276         1124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4490
            SAMEA115707157-rid22580103.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAZKAY010000276 DAZKAY010000000
VERSION     DAZKAY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707157
            Sequence Read Archive: ERR13196888
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287905.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287905.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:40:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,947
            Genes (coding)                    :: 2,859
            CDSs (with protein)               :: 2,859
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707157-rid22580103.guided.215"
                     /isolate="VRE4490"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG8WP_003006"
     CDS             24..416
                     /locus_tag="ACG8WP_003006"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9029204.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8WP_003007"
     CDS             417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8WP_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFP9029205.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          456 a           88 c          195 g          385 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggt
//
